Chueh-Yuan Kuo, Yue-Jin Wu, Yin-Cheng Hsieh, Hong-Hsiang Guan, Huei-Ju Tsai, Yi-Hung Lin, Yen-Chieh Huang, Ming-Yih Liu, Yaw-Kuen Li, Chun-Jung Chen
Chitinases (EC 3.2.1.14) are found in a broad range of organisms, including bacteria, fungi and higher plants, and play different roles depending on their origin. A chitinase from Bacillus cereus NCTU2 (ChiNCTU2) capable of hydrolyzing chitin as a carbon and nitrogen nutrient has been identified as a member of the family 18 glycoside hydrolases. ChiNCTU2 of molecular weight 36 kDa has been crystallized using the hanging-drop vapour-diffusion method. According to the diffraction of chitinase crystals at 1.10 A resolution, the crystal belongs to space group P2(1), with unit-cell parameters a = 50...
September 1, 2006: Acta Crystallographica. Section F, Structural Biology and Crystallization Communications