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Lactobacillus gasseri

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https://www.readbyqxmd.com/read/28315382/characterization-of-vaginal-lactobacillus-species-by-rplk-based-multiplex-qpcr-in-russian-women
#1
Vladimir V Demkin, Stanislav I Koshechkin
We describe a multiplex qPCR assay for identification and quantitative assessment of a set of vaginal Lactobacillus species, including L. acidophilus, L. crispatus, L. gasseri, L. helveticus, L. iners, and L. jensenii. The assay extends the previously developed qPCR method for Lactobacillus detection and total quantification based on targeting the rplK gene. Both assays use only single pair of primers and a set of probes combined in three reactions, comprising a vaginal Lactobacillus diagnostic assay panel...
March 14, 2017: Anaerobe
https://www.readbyqxmd.com/read/28282902/lactobacillus-gasseri-pa-3-uses-the-purines-imp-inosine-and-hypoxanthine-and-reduces-their-absorption-in-rats
#2
Naruomi Yamada, Chizuru Saito-Iwamoto, Marie Nakamura, Misato Soeda, Yoshika Chiba, Hiroshi Kano, Yukio Asami
Excessive intake of purine-rich foods elevates serum levels of uric acid. Animal and fish meats contain high amounts of inosine and its related purines, and the reduction of taking those purines is crucial for the improvement of serum uric acid levels. We previously showed that Lactobacillus gasseri PA-3 (PA-3) incorporates adenosine and its related purines and that oral treatment with PA-3 reduced adenosine absorption in rats. This study investigated whether PA-3 also incorporates IMP (inosine 5'-monophosphate), inosine, and hypoxanthine, and whether it reduces their absorption in rats...
March 8, 2017: Microorganisms
https://www.readbyqxmd.com/read/28228426/probiotics-lactobacillus-gasseri-ks-13-bifidobacterium-bifidum-g9-1-and-bifidobacterium-longum-mm-2-improve-rhinoconjunctivitis-specific-quality-of-life-in-individuals-with-seasonal-allergies-a-double-blind-placebo-controlled-randomized-trial
#3
Jennifer C Dennis-Wall, Tyler Culpepper, Carmelo Nieves, Cassie C Rowe, Alyssa M Burns, Carley T Rusch, Ashton Federico, Maria Ukhanova, Sheldon Waugh, Volker Mai, Mary C Christman, Bobbi Langkamp-Henken
Background: Rhinoconjunctivitis-specific quality of life is often reduced during seasonal allergies. The Mini Rhinoconjunctivitis Quality of Life Questionnaire (MRQLQ) is a validated tool used to measure quality of life in people experiencing allergies (0 = not troubled to 6 = extremely troubled). Probiotics may improve quality of life during allergy season by increasing the percentage of regulatory T cells (Tregs) and inducing tolerance.Objective: The objective of this study was to determine whether consuming Lactobacillus gasseri KS-13, Bifidobacterium bifidum G9-1, and B...
March 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28223974/determination-of-antibacterial-and-technological-properties-of-vaginal-lactobacilli-for-their-potential-application-in-dairy-products
#4
Lorenzo Siroli, Francesca Patrignani, Diana I Serrazanetti, Carola Parolin, Rogers A Ñahui Palomino, Beatrice Vitali, Rosalba Lanciotti
Functional foods could differently affect human health in relation to the gender. Recent studies have highlighted the anti-Candida and anti-Chlamydia activities of some Lactobacillus strains isolated from the vagina of healthy women. Considering these important beneficial activities on women's health, the preparation of functional food containing active vaginal lactobacilli can represent a great scientific challenge for the female gender. In this context, the aim of this work was to study some functional and technological properties of 17 vaginal strains belonging to the species Lactobacillus crispatus, Lactobacillus gasseri, and Lactobacillus vaginalis in the perspective to include them in dairy products...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28103868/probiotics-for-vaginal-health-in-south-africa-what-is-on-retailers-shelves
#5
Anna-Ursula Happel, Shameem Z Jaumdally, Tanya Pidwell, Tracy Cornelius, Heather B Jaspan, Remy Froissart, Shaun L Barnabas, Jo-Ann S Passmore
BACKGROUND: Probiotics are widely used to improve gastrointestinal (GI) health, but they may also be useful to prevent or treat gynaecological disorders, including bacterial vaginosis (BV) and candidiasis. BV prevalence is high in South Africa and is associated with increased HIV risk and pregnancy complications. We aimed to assess the availability of probiotics for vaginal health in retail stores (pharmacies, supermarkets and health stores) in two major cities in South Africa. METHODS: A two-stage cluster sampling strategy was used in the Durban and Cape Town metropoles...
January 19, 2017: BMC Women's Health
https://www.readbyqxmd.com/read/28052291/yogurt-containing-lactobacillus-gasseri-mitigates-aspirin-induced-small-bowel-injuries-a-prospective-randomized-double-blind-placebo-controlled-trial
#6
RANDOMIZED CONTROLLED TRIAL
Takayoshi Suzuki, Aya Masui, Jun Nakamura, Hirokazu Shiozawa, Jun Aoki, Hirohiko Nakae, Shingo Tsuda, Jin Imai, Ozawa Hideki, Masashi Matsushima, Tetsuya Mine, Akira Tamura, Toshihiro Ohtsu, Yukio Asami, Atsushi Takagi
BACKGROUND: Although there is evidence about the beneficial effects of probiotics, their effects on aspirin-induced small bowel injuries have not been well examined. We evaluated the effects of the probiotic Lactobacillus gasseri OLL2716 (LG) on aspirin-induced small intestinal lesions, such as ulcers, erosions, reddened lesions, and bleeding. SUMMARY: This study enrolled 64 patients who received aspirin for more than 1 month and provided written informed consent to be part of the study...
2017: Digestion
https://www.readbyqxmd.com/read/28018325/sensitive-quantitative-analysis-of-the-meconium-bacterial-microbiota-in-healthy-term-infants-born-vaginally-or-by-cesarean-section
#7
Ravinder Nagpal, Hirokazu Tsuji, Takuya Takahashi, Kazunari Kawashima, Satoru Nagata, Koji Nomoto, Yuichiro Yamashiro
For decades, babies were thought to be born germ-free, but recent evidences suggest that they are already exposed to various bacteria in utero. However, the data on population levels of such pioneer gut bacteria, particularly in context to birth mode, is sparse. We herein aimed to quantify such bacteria from the meconium of 151 healthy term Japanese infants born vaginally or by C-section. Neonatal first meconium was obtained within 24-48 h of delivery; RNA was extracted and subjected to reverse-transcription-quantitative PCR using specific primers for Clostridium coccoides group, C...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27999938/characterization-of-cinnamoyl-esterases-from-different-lactobacilli-and-bifidobacteria
#8
Caroline Fritsch, André Jänsch, Matthias A Ehrmann, Simone Toelstede, Rudi F Vogel
A high variety of plants that are used for food production contain esterified hydroxycinnamic acids. As their free forms display several benefits, like an enhanced absorption in human intestinal tract, anti-oxidative and anti-carcinogenic effects, an improved protein solubility and reduced discoloration, the microbial ability to cleave the ester bond is highly desired. In order to examine potential fermentation strains for this purpose, six different lactic acid bacteria and one bifidobacterial strain were screened for their ability to degrade esterified hydroxycinnamic acids because these strains are commonly used for fermentation of plant-based foods...
February 2017: Current Microbiology
https://www.readbyqxmd.com/read/27990138/purification-of-antilisterial-peptide-subtilosin-a-from-novel-bacillus-tequilensis-fr9-and-demonstrate-their-pathogen-invasion-protection-ability-using-human-carcinoma-cell-line
#9
Rizwana Parveen Rani, Marimuthu Anandharaj, Subramani Hema, Ramasamy Deepika, Abraham David Ravindran
This study focuses on isolation, screening, and characterization of novel probiotics from gastrointestinal tract of free-range chicken (Gallus gallus domesticus). Fifty seven colonies were isolated and three isolates (FR4, FR9, and FR12) were selected and identified as Lactobacillus gasseri FR4, Bacillus tequilensis FR9, and L. animalis FR12 by 16S rRNA sequencing. Three strains were able to survive in stimulated acidic and bile conditions and inhibit the growth of pathogens. Especially, FR9 exhibited maximum inhibition against Listeria monocytogenes and none of them exhibited hemolytic activity...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27965594/lack-of-adrenomedullin-results-in-microbiota-changes-and-aggravates-azoxymethane-and-dextran-sulfate-sodium-induced-colitis-in-mice
#10
Sonia Martínez-Herrero, Ignacio M Larrayoz, Judit Narro-Íñiguez, María J Villanueva-Millán, Emma Recio-Fernández, Patricia Pérez-Matute, José A Oteo, Alfredo Martínez
The link between intestinal inflammation, microbiota, and colorectal cancer is intriguing and the potential underlying mechanisms remain unknown. Here we evaluate the influence of adrenomedullin (AM) in microbiota composition and its impact on colitis with an inducible knockout (KO) mouse model for AM. Microbiota composition was analyzed in KO and wild type (WT) mice by massive sequencing. Colitis was induced in mice by administration of azoxymethane (AOM) followed by dextran sulfate sodium (DSS) in the drinking water...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27906630/evaluation-of-purine-utilization-by-lactobacillus-gasseri-strains-with-potential-to-decrease-the-absorption-of-food-derived-purines-in-the-human-intestine
#11
N Yamada, C Iwamoto, H Kano, N Yamaoka, T Fukuuchi, K Kaneko, Y Asami
It is well accepted that frequent and heavy intake of purine-rich foods causes elevation of serum uric acid levels, which is a risk factor of hyperuricemia. Reducing intestinal absorption of dietary purines may attenuate the elevation of serum uric acid levels and exacerbation of hyperuricemia. This reduction may be achieved by the ingestion of lactic acid bacteria that take up purines in the intestine. In this study, we investigated the degree of uptake and utilization of purines of three lactobacilli strains...
December 2016: Nucleosides, Nucleotides & Nucleic Acids
https://www.readbyqxmd.com/read/27904074/prophylactic-effect-of-lactobacillus-pentosus-strain-s-pt84-on-candida-infection-and-gastric-inflammation-in-a-murine-gastrointestinal-candidiasis-model-errata
#12
Toshihiro Maekawa, A Sanae Ishijima, Masayuki Ida, Takayuki Izumo, Yoshiko Ono, Hiroshi Shibata, Shigeru Abe
We previously showed a prophylactic effect of Lactobacillus pentosus strain S-PT84 against oral candidiasis in mice. In the present study, we evaluated the protective effect of S-PT84 against Candida infection of the gastrointestinal tract. As the first step, we used an in vitro assay to compare the inhibitory effects of several lactobacilli (S-PT84 and Lactobacillus pentosus type strain JCM1558(T), Lactobacillus gasseri type strain JCM1131(T) and Lactobacillus casei type strain JCM1134(T)) on mycelial growth of Candida albicans...
2016: Medical Mycology Journal
https://www.readbyqxmd.com/read/27904056/prophylactic-effect-of-lactobacillus-pentosus-strain-s-pt84-on-candida-infection-and-gastric-inflammation-in-a-murine-gastrointestinal-candidiasis-model
#13
Toshihiro Maekawa, A Sanae Ishijima, Masayuki Ida, Takayuki Izumo, Yoshiko Ono, Hiroshi Shibata, Shigeru Abe
We previously showed a prophylactic effect of Lactobacillus pentosus strain S-PT84 against oral candidiasis in mice. In the present study, we evaluated the protective effect of S-PT84 against Candida infection of the gastrointestinal tract. As the first step, we used an in vitro assay to compare the inhibitory effects of several lactobacilli (S-PT84 and Lactobacillus pentosus type strain JCM1558(T), Lactobacillus gasseri type strain JCM1131(T) and Lactobacillus casei type strain JCM1134(T)) on mycelial growth of Candida albicans...
2016: Medical Mycology Journal
https://www.readbyqxmd.com/read/27902241/description-of-lactobacillus-colini-sp-nov-isolated-from-northern-bobwhite-colinus-virginianus
#14
Michael Z Zhang, Ming Yang, Hongwen Su, Dale Rollins, Shuping Zhang
Biochemical and molecular studies were performed on five unknown bacterial strains isolated from the intestinal contents of Northern Bobwhites (Colinus virginianus) collected from western Texas, USA. The strains were Gram-stain-positive, catalase-negative, non-spore-forming rods arranged in single cells, pairs or short chains. Colonies on Columbia blood agar are circular, flat, entire, approximately 0.5 - 1.5 mm in diameter and surrounded with a zone of alpha hemolysis at 48 h incubation at 37 °C. Colonies on MRS agar are umbonate with irregular edge, opaque and approximately 1 - 1...
October 28, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27890610/a-novel-real-time-pcr-assay-for-highly-specific-detection-and-quantification-of-vaginal-lactobacilli
#15
Vladimir V Demkin, Stanislav I Koshechkin, Alexei Slesarev
PCR detection and quantification of vaginal lactobacilli remains problematic because of the high level of genetic heterogeneity and taxonomic complexity within the genus Lactobacillus. The aim of the present study was to identify conserved sequences among the genomes of major species of vaginal lactobacilli that could be used for the development of a PCR-based method for quantitative determination of vaginal microbiota-specific lactobacilli. Comparative analysis of the genomes of several species of vaginal lactobacilli allowed us to identify conserved regions in the rplK gene, which encodes ribosomal protein L11, and to design group-specific PCR primers and a probe for selected species from the L...
November 25, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27881419/oral-administration-of-a-select-mixture-of-bacillus-probiotics-affects-the-gut-microbiota-and-goblet-cell-function-following-escherichia-coli-challenge-in-newly-weaned-pigs-of-genotype-muc4-that-are-supposed-to-be-enterotoxigenic-e-coli-f4ab-ac-receptor-negative
#16
Wei Zhang, Yao-Hong Zhu, Dong Zhou, Qiong Wu, Dan Song, Johan Dicksved, Jiu-Feng Wang
Structural disruption of the gut microbiota and impaired goblet cell function are collateral etiologic factors in enteric diseases. Low, moderate, or high doses of a Bacillus licheniformis-B. subtilis mixture (BLS mix) were orally administered to piglets of genotype MUC4 that are supposed to be F4-expressing enterotoxigenic Escherichia coli strain (F4(+) ETEC) F4ab/ac receptor negative (i.e., MUC4-resistant piglets) for 1 week before F4(+) ETEC challenge. The luminal contents were collected from the mucosa of the colon on day 8 after F4(+) ETEC challenge...
February 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27867804/dfast-and-daga-web-based-integrated-genome-annotation-tools-and-resources
#17
Yasuhiro Tanizawa, Takatomo Fujisawa, Eli Kaminuma, Yasukazu Nakamura, Masanori Arita
Quality assurance and correct taxonomic affiliation of data submitted to public sequence databases have been an everlasting problem. The DDBJ Fast Annotation and Submission Tool (DFAST) is a newly developed genome annotation pipeline with quality and taxonomy assessment tools. To enable annotation of ready-to-submit quality, we also constructed curated reference protein databases tailored for lactic acid bacteria. DFAST was developed so that all the procedures required for DDBJ submission could be done seamlessly online...
2016: Bioscience of Microbiota, Food and Health
https://www.readbyqxmd.com/read/27824276/identification-of-a-new-small-bioactive-peptide-from-lactobacillus-gasseri-supernatant
#18
A Zanfardino, G Criscuolo, B Di Luccia, E Pizzo, M L Ciavatta, E Notomista, A Carpentieri, A Pezzella, M Varcamonti
Ten lactic acid bacteria (LAB) strains, previously isolated from human ileal biopsy of healthy volunteers, were screened for production and secretion of molecules having anti-bacterial and anti-biofilm activities. Because many recent reports indicate that LAB secreted molecules may exert immune-modulatory action, we also tested the effect on human intestinal HCT116 cells challenged with bacterial lipopolysaccharides. One of the Lactobacillus gasseri strains, SF1109, strongly inhibited: (1) Pseudomonas aeruginosa growth; (2) Escherichia coli biofilm production; (3) LPS induction of P-ERK1/2 in HCT116 cells, and was selected for further characterisation of the secreted active molecule...
November 8, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27796437/the-growth-kinetics-and-metabolic-and-antioxidant-activities-of-the-functional-synbiotic-combination-of-lactobacillus-gasseri-505-and-cudrania-tricuspidata-leaf-extract
#19
Nam Su Oh, Ji Young Lee, Younghoon Kim
In a previous study, the synbiotic combination of selected Lactobacillus gasseri strains and Cudrania tricuspidata leaf extract (CT) was shown to significantly improve the functionality of fermented milk, and the greatest synbiotic effect was exhibited with L. gasseri 505. The aim of the present study was to investigate the growth kinetics and fermentation metabolism of this specific synbiotic combination. Fermentation was carried out in synthetic media and milk with or without CT supplementation using L. gasseri 505...
December 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27776911/genomic-characterization-of-a-fructophilic-bee-symbiont-lactobacillus-kunkeei-reveals-its-niche-specific-adaptation
#20
Shintaro Maeno, Yasuhiro Tanizawa, Yu Kanesaki, Eri Kubota, Himanshu Kumar, Leon Dicks, Seppo Salminen, Junichi Nakagawa, Masanori Arita, Akihito Endo
Lactobacillus kunkeei is classified as a sole obligate fructophilic lactic acid bacterium that is found in fructose-rich niches, including the guts of honeybees. The species is differentiated from other lactobacilli based on its poor growth with glucose, enhanced growth in the presence of oxygen and other electron acceptors, and production of high concentrations of acetate from the metabolism of glucose. These characteristics are similar to phylogenetically distant Fructobacillus spp. In the present study, the genomic structure of L...
December 2016: Systematic and Applied Microbiology
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