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Lactobacillus gasseri

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https://www.readbyqxmd.com/read/29340028/high-resolution-microbiome-profiling-uncovers-fusobacterium-nucleatum-lactobacillus-gasseri-johnsonii-and-lactobacillus-vaginalis-associated-to-oral-and-oropharyngeal-cancer-in-saliva-from-hpv-positive-and-hpv-negative-patients-treated-with-surgery-and-chemo
#1
Rafael Guerrero-Preston, James Robert White, Filipa Godoy-Vitorino, Arnold Rodríguez-Hilario, Kelvin Navarro, Herminio González, Christina Michailidi, Anne Jedlicka, Sierra Canapp, Jessica Bondy, Amanda Dziedzic, Barbara Mora-Lagos, Gustavo Rivera-Alvarez, Carmen Ili-Gangas, Priscilla Brebi-Mieville, William Westra, Wayne Koch, Hyunseok Kang, Luigi Marchionni, Young Kim, David Sidransky
Microbiome studies show altered microbiota in head and neck squamous cell carcinoma (HNSCC), both in terms of taxonomic composition and metabolic capacity. These studies utilized a traditional bioinformatics methodology, which allows for accurate taxonomic assignment down to the genus level, but cannot accurately resolve species level membership. We applied Resphera Insight, a high-resolution methodology for 16S rRNA taxonomic assignment that is able to provide species-level context in its assignments of 16S rRNA next generation sequencing (NGS) data...
December 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29316868/ileal-mucosal-response-to-the-same-probiotic-lactobacillus-strains-is-markedly-different-between-suckling-and-adult-mice
#2
Ryo Inoue, Shigeru Fujiwara, Takamitsu Tsukahara
While evidence shows that probiotic supplementation exerts beneficial effects on developing children and animals, it is unclear whether it would exert equal or similar effects on adult human and animals. In this study, response to probiotic lactobacilli in ileal mucosa of suckling and adult mice was compared by evaluating gene expression profiles using DNA microarray. Two probiotic strains, Lactobacillus gasseri CP2305s and Lactobacillus plantarum CPA305C were used. Supplementation of probiotics for 7 days affected completely different genes in suckling and adult mice, regardless of the probiotic strain...
January 10, 2018: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29231915/lactobacillus-gasseri-endocarditis-on-the-aortic-valve-bioprosthesis-a-case-report
#3
Waldemar Elikowski, Małgorzata Małek-Elikowska, Monika Lisiecka, Anna Bodora, Katarzyna Wiśniewska, Zofia Oko-Sarnowska
Lactobacillus is a genus of Gram-positive, facultative anaerobic/ microaerophilic, rod-shaped bacteria. Lactobacilli constitute a significant component of the human microbiota in the oral cavity, gastrointestinal tract and female urogenital tract. They are also widely used as probiotics. Rarely, especially in patients with impaired immunity or with structural heart disease, do Lactobacilli become the pathogen responsible for serious infections, e.g. infective endocarditis (IE). CASE REPORT: The authors describe a case of an 80-year-old female with a past history of aortic valve replacement, mitral annuloplasty and pacemaker implantation admitted to hospital due to weakening, subfebrile state and chills...
November 23, 2017: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/29169948/biosynthesis-of-inulin-from-sucrose-using-inulosucrase-from-lactobacillus-gasseri-dsm-20604
#4
Dawei Ni, Yingying Zhu, Wei Xu, Yuxiang Bai, Tao Zhang, Wanmeng Mu
Inulin is composed of fructose residues connected by β-(2, 1) glycosidic linkages with many promising physiochemical and physiological properties. In this study, an inulin-producing inulosucrase gene from Lactobacillus gasseri DSM 20604 was cloned, expressed and purified. SDS-PAGE and gel filtration found that the recombinant inulosucrase is a monomeric protein with a molecular weight of 63 KDa. The optimal pH for its sucrose hydrolysis and transfructosylation activities was pH 5.5. The optimal temperatures were measured to be 45, 25, and 35°C for sucrose hydrolysis, transfructosylation, and total activity, respectively...
November 20, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29147594/lactobacillus-gasseri-causing-bilateral-empyema
#5
Angela Esquibel, Ala S Dababneh, Bharath Raj Palraj
Lactobacilli are common commensal bacteria found in the gastrointestinal and genitourinary tract. Although they are usually thought to be nonpathogenic, there have been several cases that demonstrate severe infections caused by these microorganisms. This is a case of a 49-year-old male with previously undiagnosed type two diabetes mellitus who presented with a 3-month history of cough and was found to have right sided Lactobacillus gasseri empyema for which he underwent video-assisted thoracoscopic surgery (VATS) with chest tube placement...
2017: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/29138104/novel-mixed-vesicles-containing-lactobacilli-biosurfactant-for-vaginal-delivery-of-an-anti-candida-agent
#6
Angela Abruzzo, Barbara Giordani, Carola Parolin, Beatrice Vitali, Michele Protti, Laura Mercolini, Martina Cappelletti, Stefano Fedi, Federica Bigucci, Teresa Cerchiara, Barbara Luppi
The purpose of this work was to prepare and characterize an innovative formulation for vaginal delivery of econazole nitrate, commonly used for the treatment of Candida infections. A novel biosurfactant isolated from a vaginal Lactobacillus strain was used to prepare phosphatidylcholine based mixed vesicles. Biosurfactant was produced by Lactobacillus gasseri BC9, isolated from the vagina of a healthy premenopausal woman, and was chemically characterized by FT-IR and ESI-MS. Mixed vesicles, obtained through film rehydration and extrusion method, were characterized in terms of size, zeta potential, encapsulation efficiency, mucoadhesion properties and econazole release...
November 11, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29133090/characterization-of-a-novel-exopolysaccharide-produced-by-lactobacillus-gasseri-fr4-and-demonstration-of-its-in-vitro-biological-properties
#7
Rizwana Parveen Rani, Marimuthu Anandharaj, Abraham David Ravindran
In this study, hetero-exopolysaccharide of Lactobacillus gasseri FR4 isolated from native chicken was produced and purified. The molecular weight of LgEPS was found to be 1.86×10(5)Da. The gas chromatographic analysis revealed that the LgEPS was majorly composed of glucose (65.31%), mannose (16.51%), galactose (8.45%), rhamnose (6.55%) and a small fraction of fucose (3.18%). The functional groups of LgEPS were confirmed by FT-IR analysis. The 1D ((1)H and (13)C) and 2D NMR (COSY and HSQC) analysis showed the presence of 1,6 linked-α-d-Glcp, 1,4 linked-α-d-Galp, 1,3,4 linked-α-d-Manp, 1,3 linked-α-l-Rhap, 1,4 linked-α-l-Fucp, 1,4 linked-β-d-Glcp, and β-d-Galp-1 residues...
November 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29071554/inhibitory-effect-of-vaginal-lactobacillus-supernatants-on-cervical-cancer-cells
#8
Ke-Di Wang, Dong-Jiang Xu, Bao-Ya Wang, Dong-Hui Yan, Zhi Lv, Jian-Rong Su
Lactobacilli have been shown to inhibit the proliferation of several types of cancer cells, but the effects of vaginal Lactobacilli on cervical cancer cells have seldom been reported. We incubated Caski cells with supernatants of predominant strains in the vagina and investigated their effects on cell growth and the possible mechanisms. Cell-free supernatants of Lactobacillus crispatus, L. jensenii, and L. gasseri were prepared and purified. Caski cells were treated with various concentrations of Lactobacillus supernatants (LS)...
October 25, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29065710/effect-of-a-yoghurt-drink-containing-lactobacillus-strains-on-bacterial-vaginosis-in-women-a-double-blind-randomised-controlled-clinical-pilot-trial
#9
C Laue, E Papazova, A Liesegang, A Pannenbeckers, P Arendarski, B Linnerth, K J Domig, W Kneifel, L Petricevic, J Schrezenmeir
Bacterial vaginosis (BV) is characterised by a depletion of lactobacilli in favour of an overgrowth of anaerobic bacteria. It is associated with increased risk for urogenital infections and abortion. In this study we assessed the effect of a yoghurt drink containing Lactobacillus strains on BV. The strains had been isolated from healthy pregnant women and selected for acidification capacity, production of H2O2, glycogen utilisation, bile salt tolerance and inhibition of pathogens. Using Amsel criteria BV was diagnosed in 36 women aged ≥18 years with stable menstrual cycle or menopause...
October 25, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/29038772/revealing-the-genomic-differences-between-two-subgroups-in-lactobacillus-gasseri
#10
Ipputa Tada, Yasuhiro Tanizawa, Akihito Endo, Masanori Tohno, Masanori Arita
Being an autochthonous species in humans, Lactobacillus gasseri is widely used as a probiotic for fermented products. We thoroughly compared the gene contents of 75 L. gasseri genomes and identified two intraspecific groups by the average nucleotide identity (ANI) threshold of 94%. Group I, with 48 strains, possessed 53 group-specific genes including the gassericin T cluster (9 genes) and N-acyl homoserine lactone lactonase. Group II, with 27 strains, including the type strain ATCC 33323, possessed group-specific genes with plasmid- or phage-related annotations...
2017: Bioscience of Microbiota, Food and Health
https://www.readbyqxmd.com/read/29032406/lactobacillus-gasseri-attenuates-allergic-airway-inflammation-through-ppar%C3%AE-activation-in-dendritic-cells
#11
Miao-Hsi Hsieh, Ren-Long Jan, Lawrence Shih-Hsin Wu, Pei-Chi Chen, Hui-Fang Kao, Wen-Shuo Kuo, Jiu-Yao Wang
Lactobacilli prevent the early development of allergic diseases in children and experimental asthma in mice. However, the detailed mechanism underlying this action remains unknown. We aimed to explore the activation pathway in the host by Lactobacillus and identify its immunomodulation mechanism in allergic asthma. Continuous administration of 10(7) cfu, but not 10(9) cfu, of L. gasseri for 4 weeks prevented Dermatophagoides pteronyssinus (Der p)-induced airway hypersensitivity and inflammation in a mouse model of allergic asthma...
October 14, 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/29031236/inhibitory-effect-of-probiotic-lactobacilli-supernatants-on-single-and-mixed-non-albicans-candida-species-biofilm
#12
Yulong Tan, Matthias Leonhard, Doris Moser, Su Ma, Berit Schneider-Stickler
OBJECTIVES: Oral candidiasis is one of the most common human fungal infections. While most cases of the Candida species isolated from the oral cavity are Candida albicans, a large number of candidiasis is attributed to non-albicans Candida species. In this study, we aim to evaluate the in vitro inhibition of supernatants of Lactobacillus gasseri and Lactobacillus rhamnosus on the single and mixed species biofilm of non-albicans Candida species, including Candida tropicalis, Candida krusei and Candida parapsilosis...
October 6, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28975455/potential-prebiotic-oligosaccharide-mixtures-from-acidic-hydrolysis-of-rice-bran-and-cassava-pulp
#13
Chanida Hansawasdi, Peter Kurdi
Two agricultural wastes, rice bran and cassava pulp were subjected to acidic hydrolysis by 2 M sulfuric acid which resulted in hemicellulosic oligosaccharide mixtures. Monosaccharide component analysis of these mixtures revealed that the oligosaccharides of rice bran acid hydrolysate (RAHF) composed of glucose and arabinose while cassava pulp acid hydrolysate (CAHF) was found to be comprised of glucose, galactose and arabinose. Both RAHF and CAHF were able to fuel all of the tested three Lactobacillus, five Bifidobacterium and three Bacteroides strains indicating the prebiotic potential of these oligosaccharide mixtures...
October 3, 2017: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/28948675/para-psychobiotic-lactobacillus-gasseri-cp2305-ameliorates-stress-related-symptoms-and-sleep-quality
#14
K Nishida, D Sawada, T Kawai, Y Kuwano, S Fujiwara, K Rokutan
AIMS: To confirm the stress-relieving effects of heat-inactivated, enteric-colonizing Lactobacillus gasseri CP2305 (paraprobiotic CP2305) in medical students taking a cadaver dissection course. METHODS AND RESULTS: Healthy students (21 males and 11 females) took paraprobiotic CP2305 daily for 5 weeks during a cadaver dissection course. The General Health Questionnaire and the Pittsburgh Sleep Quality Index were employed to assess stress-related somatic symptoms and sleep quality respectively...
September 26, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28945188/characterization-of-vaginal-lactobacilli-from-hiv-negative-and-hiv-positive-indian-women-and-their-association-with-genital-hiv-1-shedding
#15
Arati Mane, Mansa Angadi, Pallavi Vidhate, Shilpa Bembalkar, Ishrat Khan, Shubhangi Bichare, Manisha Ghate, Madhuri Thakar
One of the crucial determinants for successful administration of lactobacilli to the vaginal niche is the use of appropriate Lactobacillus species. In this cross-sectional study 54 human immunodeficiency virus (HIV)-negative and 76 HIV-positive antiretroviral treatment-naïve women were evaluated for culturable vaginal lactobacilli and their association with genital HIV-1 shedding. Lactobacillus species were identified by 16S rDNA sequencing while cervical and plasma HIV-1 viral load was determined by Abbott real-time PCR...
October 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28942850/characterization-and-complete-genome-sequences-of-l-rhamnosus-dsm-14870-and-l-gasseri-dsm-14869-contained-in-the-ecovag-%C3%A2-probiotic-vaginal-capsules
#16
Harold Marcotte, Kasper Krogh Andersen, Yin Lin, Fanglei Zuo, Zhu Zeng, Per Göran Larsson, Erik Brandsborg, Gunnar Brønstad, Lennart Hammarström
Lactobacillus rhamnosus DSM 14870 and Lactobacillus gasseri DSM 14869 were previously isolated from the vaginal epithelial cells (VEC) of healthy women and selected for the development of the vaginal EcoVag(®) probiotic capsules. EcoVag(®) was subsequently shown to provide long-term cure and reduce relapse of bacterial vaginosis (BV) as an adjunct to antibiotic therapy. To identify genes potentially involved in probiotic activity, we performed genome sequencing and characterization of the two strains. The complete genome analysis of both strains revealed the presence of genes encoding functions related to adhesion, exopolysaccharide (EPS) biosynthesis, antimicrobial activity, and CRISPR adaptive immunity but absence of antibiotic resistance genes...
December 2017: Microbiological Research
https://www.readbyqxmd.com/read/28927409/biological-and-physicochemical-properties-of-biosurfactants-produced-by-lactobacillus-jensenii-p6a-and-lactobacillus-gasseri-p65
#17
I M C Morais, A L Cordeiro, G S Teixeira, V S Domingues, R M D Nardi, A S Monteiro, R J Alves, E P Siqueira, V L Santos
BACKGROUND: Lactobacillus species produce biosurfactants that can contribute to the bacteria's ability to prevent microbial infections associated with urogenital and gastrointestinal tracts and the skin. Here, we described the biological and physicochemical properties of biosurfactants produced by Lactobacillus jensenii P6A and Lactobacillus gasseri P65. RESULTS: The biosurfactants produced by L. jensenii P6A and L. gasseri P65 reduced the water surface tension from 72 to 43...
September 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28911639/designing-primers-and-evaluation-of-the-efficiency-of-propidium-monoazide-quantitative-polymerase-chain-reaction-for-counting-the-viable-cells-of-lactobacillus-gasseri-and-lactobacillus-salivarius
#18
Chieh-Hsien Lai, Sih-Rong Wu, Jen-Chieh Pang, Latha Ramireddy, Yu-Cheng Chiang, Chien-Ku Lin, Hau-Yang Tsen
The purpose of this study is to evaluate the efficiency of using propidium monoazide (PMA) real-time quantitative polymerase chain reaction (qPCR) to count the viable cells of Lactobacillus gasseri and Lactobacillus salivarius in probiotic products. Based on the internal transcription spacer and 23S rRNA genes, two primer sets specific for these two Lactobacillus species were designed. For a probiotic product, the total deMan Rogosa Sharpe plate count was 8.65±0.69 log CFU/g, while for qPCR, the cell counts of L...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28878691/spontaneous-preterm-birth-is-associated-with-differential-expression-of-vaginal-metabolites-by-lactobacilli-dominated-microflora
#19
Graham P Stafford, Jennifer L Parker, Emmanuel Amabebe, James Kistler, Steven Reynolds, Victoria Stern, Martyn Paley, Dilly O C Anumba
A major challenge in preventing preterm birth (PTB) is identifying women at greatest risk. This pilot study prospectively examined the differences in vaginal microbiota and metabolite profiles of women who delivered prematurely compared to their term counterparts in a cohort of asymptomatic (studied at 20-22, n = 80; and 26-28 weeks, n = 41) and symptomatic women (studied at 24-36 weeks, n = 37). Using 16S rRNA sequencing, the vaginal microbiota from cervicovaginal fluid samples was characterized into five Community State Types (CST) dominated by Lactobacillus spp...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28798730/characterization-of-the-culturable-subpopulations-of-lactobacillus-in-the-chicken-intestinal-tract-as-a-resource-for-probiotic-development
#20
Bishnu Adhikari, Young M Kwon
To gain better understanding of the distributions of the culturable Lactobacillus species in the chicken intestinal tract, we collected ceca, and distal ileum from 10 3-weeks-old broiler chickens. Lactobacillus strains from cecal lumen contents (M-CL), and those associated with mucosa of ceca (M-CM) and ileum (M-IM) were recovered on de Man, Rogosa and Sharpe (MRS) agar plates, and used for microbiota analysis. The total cecal content (T-CL) was also used directly for microbiota analysis. We purposefully focused on MRS-recovered populations to gain understanding of the culturable subpopulations of Lactobacillus, since the culturability is an important phenotype in order to exploit the chicken gut microbiota as a resource for development of probiotics...
2017: Frontiers in Microbiology
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