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https://www.readbyqxmd.com/read/27933650/up-regulation-of-microrna-133a-inhibits-the-mek-erk-signaling-pathway-to-promote-cell-apoptosis-and-enhance-radio-sensitivity-by-targeting-egfr-in-esophageal-cancer-in-vivo-and-in-vitro
#1
Qing-Shan Yang, Li-Peng Jiang, Chun-Yan He, Yu-Na Tong, Yuan-Yuan Liu
This study aims to explore how microRNA-133a (miR-133a) affects cell apoptosis and radio-sensitivity by targeting EGFR via regulating MEK/ERK pathway in esophageal cancer (EC). A total of 358 EC patients were selected and assigned into the resistant and sensitive groups. Human EC KYSE 150 cell line was assigned into the blank, negative control (NC), miR-133a mimic, miR-133a inhibitors, si-EGFR, miR-133a inhibitors + si-EGFR groups after transfection. MiR-133a and EGFR mRNA expressions were detected by qRT-PCR and EGFR, MEK/ERK pathway-related protein expressions were detected by Western blotting...
December 8, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27933576/-medical-certificates-and-examinations-in-family-doctor-s-office-indications-barriers-and-relevance-of-standardization
#2
Livia Ertl, Linda Sanftenberg, Jörg Schelling
BACKGROUND: In view of medical care being required more and more, the doctors' tight time should be reserved for tasks which correspond to their qualification. A standardization of certificates might save time and improve quality. OBJECTIVE: This paper overviews the role of certificates and the required examinations in family doctor's everyday practice. Current practices, difficulties and suggestions for improvements were worked out in order to illuminate the potential for standardization...
December 2016: MMW Fortschritte der Medizin
https://www.readbyqxmd.com/read/27933500/climate-change-mitigation-the-potential-of-agriculture-as-a-renewable-energy-source-in-nigeria
#3
Z A Elum, D M Modise, G Nhamo
Energy is pivotal to the economic development of every nation. However, its production and utilization leads to undesirable carbon emissions that aggravate global warming which results in climate change. The agriculture sector is a significant user of energy. However, it has the potential to be a major contributor to Nigeria's energy supply mix in meeting its energy deficit. More so, in the light of current and impending adverse effects of climate change, there is a need to contain GHG's emissions. This paper focuses on bioenergy utilization as a climate change mitigation strategy and one that can, through effective waste management, enhance sustainable economic development in Nigeria...
December 9, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27933336/shp-1-activation-inhibits-vascular-smooth-muscle-cell-proliferation-and-intimal-hyperplasia-in-a-rodent-model-of-insulin-resistance-and-diabetes
#4
Weier Qi, Qian Li, Chong Wee Liew, Christian Rask-Madsen, Samuel M Lockhart, Lars Melholt Rasmussen, Yu Xia, Xuanchun Wang, Mogher Khamaisi, Kevin Croce, George L King
AIMS/HYPOTHESIS: Accelerated migration and proliferation of vascular smooth muscle cells (VSMCs) enhances arterial restenosis after angioplasty in insulin resistance and diabetes. Elevation of Src homology 2-containing protein tyrosine phosphatase 1 (SHP-1) induces apoptosis in the microvasculature. However, the role of SHP-1 in intimal hyperplasia and restenosis has not been clarified in insulin resistance and diabetes. METHODS: We used a femoral artery wire injury mouse model, rodent models with insulin resistance and diabetes, and patients with type 2 diabetes...
December 9, 2016: Diabetologia
https://www.readbyqxmd.com/read/27933114/instantaneous-formation-of-siox-nanocomposite-for-high-capacity-lithium-ion-batteries-by-enhanced-disproportionation-reaction-during-plasma-spray-physical-vapor-deposition
#5
Tohru Tashiro, Masashi Dougakiuchi, Makoto Kambara
Nanocomposite SiOx particles have been produced by a single step plasma spray physical vapor deposition (PS-PVD) through rapid condensation of SiO vapors and the subsequent disproportionation reaction. Core-shell nanoparticles, in which 15 nm crystalline Si is embedded within the amorphous SiOx matrix, form under typical PS-PVD conditions, while 10 nm amorphous particles are formed when processed with an increased degree of non-equilibrium effect. Addition of CH4 promotes reduction in the oxygen content x of SiO x , and thereby increases the Si volume in a nanocomposite particle...
2016: Science and Technology of Advanced Materials
https://www.readbyqxmd.com/read/27933078/citrus-leprosis-virus-c-infection-results-in-hypersensitive-like-response-suppression-of-the-ja-et-plant-defense-pathway-and-promotion-of-the-colonization-of-its-mite-vector
#6
Gabriella D Arena, Pedro L Ramos-González, Maria A Nunes, Marcelo Ribeiro-Alves, Luis E A Camargo, Elliot W Kitajima, Marcos A Machado, Juliana Freitas-Astúa
Leprosis is a serious disease of citrus caused by Citrus leprosis virus C (CiLV-C, genus Cilevirus) whose transmission is mediated by false spider mites of the genus Brevipalpus. CiLV-C infection does not systemically spread in any of its known host plants, thus remaining restricted to local lesions around the feeding sites of viruliferous mites. To get insight into this unusual pathosystem, we evaluated the expression profiles of genes involved in defense mechanisms of Arabidopsis thaliana and Citrus sinensis upon infestation with non-viruliferous and viruliferous mites by using reverse-transcription qPCR...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27933075/a-non-specific-setaria-italica-lipid-transfer-protein-gene-plays-a-critical-role-under-abiotic-stress
#7
Yanlin Pan, Jianrui Li, Licong Jiao, Cong Li, Dengyun Zhu, Jingjuan Yu
Lipid transfer proteins (LTPs) are a class of cysteine-rich soluble proteins having small molecular weights. LTPs participate in flower and seed development, cuticular wax deposition, also play important roles in pathogen and abiotic stress responses. A non-specific LTP gene (SiLTP) was isolated from a foxtail millet (Setaria italica) suppression subtractive hybridization library enriched for differentially expressed genes after abiotic stress treatments. A semi-quantitative reverse transcriptase PCR analysis showed that SiLTP was expressed in all foxtail millet tissues...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27932980/tnf-%C3%AE-induced-cpla2-expression-via-nadph-oxidase-reactive-oxygen-species-dependent-nf-%C3%AE%C2%BAb-cascade-on-human-pulmonary-alveolar-epithelial-cells
#8
Chih-Chung Lin, Wei-Ning Lin, Rou-Ling Cho, Chen-Yu Wang, Li-Der Hsiao, Chuen-Mao Yang
Tumor necrosis factor-α (TNF-α) triggers activation of cytosolic phospholipase A2 (cPLA2) and then enhancing the synthesis of prostaglandin (PG) in inflammatory diseases. However, the detailed mechanisms of TNF-α induced cPLA2 expression were not fully defined in human pulmonary alveolar epithelial cells (HPAEpiCs). We found that TNF-α-stimulated increases in cPLA2 mRNA (5.2 folds) and protein (3.9 folds) expression, promoter activity (4.3 folds), and PGE2 secretion (4.7 folds) in HPAEpiCs, determined by Western blot, real-time PCR, promoter activity assay and PGE2 ELISA kit...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27932942/loss-of-fractalkine-signaling-exacerbates-axon-transport-dysfunction-in-a-chronic-model-of-glaucoma
#9
Kevin T Breen, Sarah R Anderson, Michael R Steele, David J Calkins, Alejandra Bosco, Monica L Vetter
Neurodegeneration in glaucoma results in decline and loss of retinal ganglion cells (RGCs), and is associated with activation of myeloid cells such as microglia and macrophages. The chemokine fractalkine (FKN or Cx3cl1) mediates communication from neurons to myeloid cells. Signaling through its receptor Cx3cr1 has been implicated in multiple neurodegenerative diseases, but the effects on neuronal pathology are variable. Since it is unknown how FKN-mediated crosstalk influences RGC degeneration in glaucoma, we assessed this in a chronic mouse model, DBA/2J...
2016: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/27932879/study-of-a-new-bone-targeting-titanium-implant-bone-interface
#10
Xiangning Liu, Ye Zhang, Shaobing Li, Yayu Wang, Ting Sun, Zejian Li, Lizhao Cai, Xiaogang Wang, Lei Zhou, Renfa Lai
New strategies involving bone-targeting titanium (Ti) implant-bone interface are required to enhance bone regeneration and osseointegration for orthopedic and dental implants, especially in osteoporotic subjects. In this study, a new dual-controlled, local, bone-targeting delivery system was successfully constructed by loading tetracycline-grafted simvastatin (SV)-loaded polymeric micelles in titania nanotube (TNT) arrays, and a bone-targeting Ti implant-bone interface was also successfully constructed by implanting the delivery system in vivo...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27932789/the-maintenance-ability-and-ca-2-availability-of-skeletal-muscle-are-enhanced-by-sildenafil
#11
Mei Huang, Keon Jin Lee, Kyung-Jin Kim, Mi Kyoung Ahn, Chung-Hyun Cho, Do Han Kim, Eun Hui Lee
Sildenafil relaxes vascular smooth muscle cells and is used to treat pulmonary artery hypertension as well as erectile dysfunction. However, the effectiveness of sildenafil on skeletal muscle and the benefit of its clinical use have been controversial, and most studies focus primarily on tissues and organs from disease models without cellular examination. Here, the effects of sildenafil on skeletal muscle at the cellular level were examined using mouse primary skeletal myoblasts (the proliferative form of skeletal muscle stem cells) and myotubes, along with single-cell Ca(2+) imaging experiments and cellular and biochemical studies...
December 9, 2016: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/27932760/-satb1-promotes-the-malignant-of-human-non-hodgkin-lymphoma-by-activating-the-ribonucleotide-reductase-%C3%A2-small-subunit-m2
#12
Dayong Yan, Wei Wang
To explore the role of the special AT rich sequence binding protein-1 (SATB1) and ribonucleotide reductase M2 (RRM2) in enhancing malignant progression of non-Hodgkin lymphoma (NHL). 
 Methods: A total of 42 NHL and 42 chronic lymphadenitis patients were recruited. The protein expressions of SATB1 and RRM2 in cervical lymph nodes were determined by Western blot. After overexpression of SATB1, siSATB1 or siRRM2, the mRNA levels of SATB1 and RRM2 in cells were analyzed via RT-PCR, the cell proliferation was evaluated via MTT and EdU assays, while the migration and invasion of cells were assessed by transwell assays...
November 28, 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/27932702/incorporation-of-cerium-oxide-into-hydroxyapatite-coating-regulates-osteogenic-activity-of-mesenchymal-stem-cell-and-macrophage-polarization
#13
Kai Li, Qingyi Shen, Youtao Xie, Mingyu You, Liping Huang, Xuebin Zheng
Biomedical coatings for orthopedic implants should facilitate osseointegration and mitigate implant-induced inflammatory reactions. Cerium oxide (CeO2) ceramics possess anti-oxidative properties and can be used to decrease mediators of inflammation, which makes them attractive for biomedical applications. In our work, two kinds of CeO2 incorporated hydroxyapatite coatings (HA-10Ce and HA-30Ce) were prepared via plasma spraying technique and the effects of CeO2 addition on the responses of bone mesenchymal stem cells (BMSCs) and RAW264...
December 8, 2016: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/27932520/myeloid-derived-nf-%C3%AE%C2%BAb-negative-regulation-of-pu-1-and-c-ebp-%C3%AE-driven-pro-inflammatory-cytokine-production-restrains-lps-induced-shock
#14
Simone Vanoni, Yi-Ting Tsai, Amanda Waddell, Lisa Waggoner, Jared Klarquist, Senad Divanovic, Kasper Hoebe, Kris A Steinbrecher, Simon P Hogan
Sepsis is a life-threatening event predominantly caused by Gram-negative bacteria. Bacterial infection causes a pronounced macrophage (MΦ) and dendritic cell activation that leads to excessive pro-inflammatory cytokine IL-1β, IL-6 and TNF-α production (cytokine storm), resulting in endotoxic shock. Previous experimental studies have revealed that inhibiting NF-κB signaling ameliorates disease symptoms; however, the contribution of myeloid p65 in endotoxic shock remains elusive. In this study, we demonstrate increased mortality in mice lacking p65 in the myeloid lineage (p65(Δmye)) compared with wild type mice upon ultra-pure LPS challenge...
December 8, 2016: Innate Immunity
https://www.readbyqxmd.com/read/27932455/single-cell-profiling-reveals-that-erna-accumulation-at-enhancer-promoter-loops-is-not-required-to-sustain-transcription
#15
Samir Rahman, Cornelia E Zorca, Tatiana Traboulsi, Emmanuel Noutahi, Matthew R Krause, Sylvie Mader, Daniel Zenklusen
Enhancers are intergenic DNA elements that regulate the transcription of target genes in response to signaling pathways by interacting with promoters over large genomic distances. Recent studies have revealed that enhancers are bi-directionally transcribed into enhancer RNAs (eRNAs). Using single-molecule fluorescence in situ hybridization (smFISH), we investigated the eRNA-mediated regulation of transcription during estrogen induction in MCF-7 cells. We demonstrate that eRNAs are localized exclusively in the nucleus and are induced with similar kinetics as target mRNAs...
December 8, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27932441/trophoblast-cells-inhibit-neutrophil-extracellular-trap-formation-and-enhance-apoptosis-through-vasoactive-intestinal-peptide-mediated-pathways
#16
Guillermina Calo, Florencia Sabbione, Daiana Vota, Daniel Paparini, Rosanna Ramhorst, Analía Trevani, Claudia Pérez Leirós
STUDY QUESTION: Do human trophoblast cells modulate neutrophil extracellular trap (NET) formation, reactive oxygen species (ROS) synthesis and neutrophil apoptosis through mechanisms involving vasoactive intestinal peptide (VIP)? SUMMARY ANSWER: Trophoblast cells inhibited NET formation and ROS synthesis and enhanced neutrophil apoptosis through VIP-mediated pathways in a model of maternal-placental interaction. WHAT IS KNOWN ALREADY: Immune homeostasis maintenance at the maternal-placental interface is mostly coordinated by trophoblast cells...
December 7, 2016: Human Reproduction
https://www.readbyqxmd.com/read/27932253/regulation-of-cyclin-d1-by-arsenic-and-microrna-inhibits-adipogenesis
#17
Kevin Beezhold, Linda R Klei, Aaron Barchowsky
Low-dose chronic exposure to arsenic in drinking water represents a global public health concern with established risks for metabolic and cardiovascular disease, as well as cancer. While the linkage between arsenic and disease is strong, further understanding of the molecular mechanisms of its pathogenicity is required. Previous reports demonstrated the ability of arsenic to interfere with adipogenesis, which may mediate its effects in promoting metabolic disease. We hypothesized that microRNA are important regulators of most if not all mesenchymal stem cell processes that are dysregulated by arsenic exposure to impair lipogenesis...
December 5, 2016: Toxicology Letters
https://www.readbyqxmd.com/read/27932034/oncogenic-retinoic-acid-receptor-%C3%AE-promotes-human-colorectal-cancer-growth-through-simultaneously-regulating-p21-transcription-and-gsk3%C3%AE-%C3%AE-catenin-signaling
#18
Gui-Li Huang, Wei Zhang, Hong-Yue Ren, Pan Zhou, Yun Chen, Qing-Xi Chen, Dong-Yan Shen
Retinoic acid receptor α (RARα) plays important roles in the progression of several cancers such as leukemia, breast cancer, and lung cancer. In this study, we demonstrated that RARα protein was frequently overexpressed in human CRC specimens and CRC cell lines. RARα knockdown decreased cell survival, proliferation, and colony formation in vitro and tumorigenic potential in nude mice. Specifically, RARα knockdown inhibited cell cycle progression at G1 phase through upregulation of cell cycle inhibitor p21, and downregulation of cyclinD1...
December 5, 2016: Cancer Letters
https://www.readbyqxmd.com/read/27931518/vitamin-c-enhances-the-expression-of-il17-in-a-jmjd2-dependent-manner
#19
Mi Hye Song, Varun Sasidharan Nair, Kwon Ik Oh
Previously, we reported that vitamin C facilitates the CpG demethylation of Foxp3 enhancer in CD4+Foxp3+ regulatory T cells (Tregs) by enhancing the activity of a DNA demethylase ten-eleven-translocation (Tet). However, it is not clear whether vitamin C affects other helper T cell lineages like T helper type 17 (Th17) cells which are related with Tregs. Here, we show that the expression of interleukin-17A (IL17) increases with the treatment of vitamin C but not with other antioxidants. Interestingly, the upregulation of IL17 was not accompanied by DNA demethylation in Il17 promoter and was independent of Tet enzymes...
December 9, 2016: BMB Reports
https://www.readbyqxmd.com/read/27931313/behavioral-effects-of-an-enhanced-enrichment-program-for-group-housed-sooty-mangabeys-cercocebus-atys
#20
Jessica Crast, Mollie A Bloomsmith, Trina J Jonesteller
Evaluating the behavioral effects of enrichment on animals housed in biomedical facilities is necessary to effectively support their care and wellbeing. We tested the cumulative effects of an enhanced enrichment program on sooty mangabey behavior: locomotion, feeding and foraging, manipulating items in the enclosure, social affiliation, aggression, and abnormal behavior. The enhanced enrichment program included the addition of a substrate (timothy hay), widely distributing small pieces of produce and a forage mixture in the hay, adding structures and perching, and increasing the variety of food items, foraging devices, and other manipulable items...
November 2016: Journal of the American Association for Laboratory Animal Science: JAALAS
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