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https://www.readbyqxmd.com/read/28741612/an-information-theoretic-framework-reveals-a-tunable-allosteric-network-in-group-ii-chaperonins
#1
Tom Lopez, Kevin Dalton, Anthony Tomlinson, Vijay Pande, Judith Frydman
ATP-dependent allosteric regulation of the ring-shaped group II chaperonins remains ill defined, in part because their complex oligomeric topology has limited the success of structural techniques in suggesting allosteric determinants. Further, their high sequence conservation has hindered the prediction of allosteric networks using mathematical covariation approaches. Here, we develop an information theoretic strategy that is robust to residue conservation and apply it to group II chaperonins. We identify a contiguous network of covarying residues that connects all nucleotide-binding pockets within each chaperonin ring...
July 24, 2017: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/28741319/key-genes-of-the-sex-pheromone-biosynthesis-pathway-in-female-moths-are-required-for-pheromone-quality-and-possibly-mediate-olfactory-plasticity-in-conspecific-male-moths-in-spodoptera-litura
#2
X Lin, B Wang, Y Du
Ninety sex pheromone biosynthesis genes in Spodoptera litura were identified in transcriptome data and were investigated and classified into the following five groups: fatty acid synthase, beta oxidase, fatty acyl-coenzyme A (CoA) reductase, desaturase and acetyl-CoA acetyltransferase. Fourteen female-specific genes were identified through semi-quantitative PCR, and 15 additional genes had expression levels that were 3- to 10-fold higher in females than in males. The majority of the genes had higher expression levels in the sex pheromone glands...
July 24, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28740767/the-effect-of-the-timing-of-radiotherapy-on-clinical-and-patient-reported-outcomes-after-latissimus-dorsi-breast-reconstruction-a-10-year-study
#3
Lashan J Peiris, Nicola C Dawson, Siobhan A M Laws, Richard M Rainsbury
BACKGROUND: Breast reconstruction (BR) is considered to be adversely affected by radiotherapy (RT), particularly when an implant is used. The aim of this study was to compare clinical and patient-reported outcomes after expander-assisted latissimus dorsi breast reconstruction depending on the timing of RT. METHODS: Patients undergoing BR over a 10-year period (follow-up mean, 56 [14-134] months) were divided into 3 groups. Group 1, RT after mastectomy and BR, Group 2, RT before mastectomy and BR, and Group 3, RT after mastectomy but before BR...
June 2017: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/28739375/oxidative-stress-and-inhibition-of-nitric-oxide-generation-underlie-methotrexate-induced-senescence-in-human-colon-cancer-cells
#4
Magdalena Dabrowska, Lukasz Uram, Zbigniew Zielinski, Wojciech Rode, Ewa Sikora
The response of human colon cancer C85 cells to methotrexate takes the form of reversible growth arrest of the type of stress-induced senescence. In the present study it is shown that during C85 cell progression into methotrexate-induced senescence, dihydrofolate reductase, the primary intracellular target for the drug, is stabilized at the protein level and its enzymatic activity, assayed in crude cellular extracts, decreases by 2-fold. Dihydrofolate reductase inhibition results in an increase in dihydrobiopterin level and an ultimate decrease in the tetrahydrobiopterin: dihydrobiopterin ratio in senescent cells...
July 21, 2017: Mechanisms of Ageing and Development
https://www.readbyqxmd.com/read/28738303/bio-accumulation-of-lanthanum-from-lanthanum-modified-bentonite-treatments-in-lake-restoration
#5
G Waajen, F van Oosterhout, M Lürling
Lanthanum (La) modified bentonite (LMB) is one of the available mitigating agents used for the reduction of the phosphorus (P) recycling in eutrophic lakes. The potential toxicity of the La from LMB to aquatic organisms is a matter of concern. In this study the accumulation of La was investigated in the macrophyte Elodea nuttallii, in chironomid larvae and in several fish species during periods up to five years following in situ LMB applications. The application of LMB increased the La concentration of exposed plants and animals...
July 21, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28733999/retinyl-propionate-and-climbazole-combination-demonstrates-clinical-improvement-to-the-appearance-of-hyperpigmentation-and-deep-wrinkling-with-minimal-irritation
#6
Stacy Hawkins, Jean Adamus, Chung-Yi Chiang, Edwin Covell, Jaime O'Leary, Jian-Ming Lee
OBJECTIVE: To evaluate a combination of retinyl propionate and climbazole (RPC) compared to 0.1% retinol for its efficacy, tolerance and aging appearance. METHOD: Forty-five healthy Caucasian females, ages 40-70, with moderately photodamaged facial skin, were recruited for a 16 week randomized, double-blind, IRB approved facial study. The efficacy of RPC treatment was compared to 0.1% retinol, in the same product base formulation, with twice daily, split-face product application...
July 22, 2017: International Journal of Cosmetic Science
https://www.readbyqxmd.com/read/28731469/novel-chaperonins-are-prevalent-in-the-virioplankton-and-demonstrate-links-to-viral-biology-and-ecology
#7
Rachel L Marine, Daniel J Nasko, Jeffrey Wray, Shawn W Polson, K Eric Wommack
Chaperonins are protein-folding machinery found in all cellular life. Chaperonin genes have been documented within a few viruses, yet, surprisingly, analysis of metagenome sequence data indicated that chaperonin-carrying viruses are common and geographically widespread in marine ecosystems. Also unexpected was the discovery of viral chaperonin sequences related to thermosome proteins of archaea, indicating the presence of virioplankton populations infecting marine archaeal hosts. Virioplankton large subunit chaperonin sequences (GroELs) were divergent from bacterial sequences, indicating that viruses have carried this gene over long evolutionary time...
July 21, 2017: ISME Journal
https://www.readbyqxmd.com/read/28731332/tuning-the-cavity-size-and-chirality-of-self-assembling-3d-dna-crystals
#8
Chad R Simmons, Fei Zhang, Tara MacCulloch, Nour Eddine Fahmi, Nicholas Stephanopoulos, Yan Liu, Nadrian C Seeman, Hao Yan
The foundational goal of structural DNA nanotechnology-the field that uses oligonucleotides as a molecular building block for the programmable self-assembly of nanostructured systems-was to use DNA to construct three-dimensional (3D) lattices for solving macromolecular structures. The programmable nature of DNA makes it an ideal system for rationally constructing self-assembled crystals, and immobilizing guest molecules in a repeating 3D array through their specific stereospatial interactions with the scaffold...
July 21, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28728415/-177-lu-labelled-cyclic-minigastrin-analogues-with-therapeutic-activity-in-cck2r-expressing-tumours-preclinical-evaluation-of-a-kit-formulation
#9
Christine Rangger, Maximilian Klingler, Lajos Balogh, Zita Pöstényi, Andras Polyak, Dariusz Pawlak, Renata Mikołajczak, Elisabeth von Guggenberg
Minigastrin (MG) analogues specifically target cholecystokinin-2 receptors (CCK2R) expressed in different tumours and enable targeted radiotherapy of advanced and disseminated disease when radiolabelled with a beta emitter such as (177)Lu. Especially truncated MG analogues missing the penta-Glu sequence are associated with low kidney retention and seem therefore most promising for therapeutic use. Based on [D-Glu1,desGlu2-6]MG (MG11) we have designed the two cyclic MG analogues cyclo1,9[γ-D-Glu1,desGlu2-6,D-Lys9]MG (cyclo-MG1) and cyclo1,9[γ-D-Glu1,desGlu2-6,D-Lys9,Nle11]MG (cyclo-MG2)...
July 20, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28727189/inversion-of-cpadh5-enantiopreference-and-altered-chain-length-specificity-for-methyl-3-hydroxyalkanoates
#10
Yunus Ensari, Gaurao V Dhoke, Mehdi D Davari, Marco Bocola, Anna Joelle Ruff, Ulrich Schwaneberg
Expanding the substrate scope of enzymes opens up new routes for synthesis of valuable chemicals. Ketone-functionalized fatty acid derivatives and corresponding chiral alcohols are valuable building blocks for the synthesis of a variety of chemicals including pharmaceuticals. The alcohol dehydrogenase from Candida parapsilosis (cpADH5) catalyzes the reversible oxidations of chiral alcohols and has a broad substrate range; a challenge for cpADH5 is to convert alcohols with small substituents (methyl or ethyl) next to the oxidized alcohol moiety...
July 20, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28723105/electroosmotic-effects-on-sample-concentration-at-the-interface-of-a-micro-nanochannel
#11
Honggu Chun
Electroosmotic effect on electrokinetic trapping of analytes was investigated at the micro/nanochannel interface for a series of 1-D glass nanochannels with depths of 72, 54, 29, and 9 nm. The electric double layer approaches overlap conditions as the nanochannel depth decreases suppressing the electroosmotic flow. The nanochannels' electroosmotic flow (μeo(nano) ) were determined and compared to the analyte's (fluorescein) electrophoretic mobility (μep). For the instances where μeo(nano) > μep, the analytes were concentrated on the anodic side of the nanochannel (72, 54, and 29 nm deep channels) whereas when μeo(nano) < μep, the analyte was concentrated on the cathodic side of the nanochannel (9 nm deep channel)...
July 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28722509/adaptation-of-mrna-structure-to-control-protein-folding
#12
Guilhem Faure, Aleksey Y Ogurtsov, Svetlana A Shabalina, Eugene V Koonin
Comparison of mRNA and protein structures shows that highly structured mRNAs typically encode compact protein domains suggesting that mRNA structure controls protein folding. This function is apparently performed by distinct structural elements in the mRNA, which implies 'fine tuning' of mRNA structure under selection for optimal protein folding. We find that, during evolution, changes in the mRNA folding energy follow amino acid replacements, reinforcing the notion of an intimate connection between the structures of a mRNA and the protein it encodes, and the double encoding of protein sequence and folding in the mRNA...
July 19, 2017: RNA Biology
https://www.readbyqxmd.com/read/28722398/fractionation-and-concentration-of-high-salinity-textile-wastewater-using-an-ultra-permeable-sulfonated-thin-film-composite
#13
Meng Li, Yujian Yao, Wen Zhang, Junfeng Zheng, Xuan Zhang, Lianjun Wang
A sulfonated thin-film composite (TFC) nanofiltration membrane was fabricated using 2,2'-benzidinedisulfonic acid (BDSA) and trimesoyl chloride (TMC) on a polyether sulfone substrate by conventional interfacial polymerization. Due to a nascent barrier layer with a loose architecture, the obtained TFC-BDSA-0.2 membrane showed an ultra-high pure water permeability of 48.1 ± 2.1 L-1 m-2 h-1 bar-1, and a considerably low NaCl retention ability of <1.8% over a concentration range of 10-100 g L-1. The membrane, which possesses a negatively charged surface, displayed an excellent rejection of over 99% towards Congo red (CR), and allowed the fast fractionation of high-salinity textile wastewater...
July 19, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28721137/beneficial-effects-of-n-3-polyunsaturated-fatty-acids-on-adiponectin-levels-and-adipor-gene-expression-in-patients-with-type-2-diabetes-mellitus-a-randomized-placebo-controlled-double-blind-clinical-trial
#14
Maryam Mazaherioun, Ahmad Saedisomeolia, Mohammad Hassan Javanbakht, Fariba Koohdani, Mohammad Reza Eshraghian, Mahmoud Djalali
INTRODUCTION: There is evidence that n-3 polyunsaturated fatty acids (n-3 PUFAs) exert beneficial effects to improve type 2 diabetes mellitus (T2DM), but its complications remain poorly understood. Hypoadiponectinemia is one of the important mechanisms responsible for T2DM which necessitates developing novel therapeutic strategies. We aimed to determine the effect of n-3 PUFA supplementation on circulating adiponectin and mRNA expression of adiponectin receptors (AdipoR1, AdipoR2) and Sirt-1 in T2DM patients...
June 2017: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/28719630/oligo-and-dsdna-mediated-genome-editing-using-a-teta-dual-selection-system-in-escherichia-coli
#15
Young Shin Ryu, Sathesh-Prabu Chandran, Kyungchul Kim, Sung Kuk Lee
The ability to precisely and seamlessly modify a target genome is needed for metabolic engineering and synthetic biology techniques aimed at creating potent biosystems. Herein, we report on a promising method in Escherichia coli that relies on the insertion of an optimized tetA dual selection cassette followed by replacement of the same cassette with short, single-stranded DNA (oligos) or long, double-stranded DNA and the isolation of recombinant strains by negative selection using NiCl2. This method could be rapidly and successfully used for genome engineering, including deletions, insertions, replacements, and point mutations, without inactivation of the methyl-directed mismatch repair (MMR) system and plasmid cloning...
2017: PloS One
https://www.readbyqxmd.com/read/28715715/platinum-ii-and-palladium-ii-complexes-of-tridentate-hydrazone-based-ligands-as-selective-guanine-quadruplex-binders
#16
Alexander Schmidt, Rweetuparna Guha, Alexander Hepp, Jens Müller
Several tridentate hydrazone-based ligands, synthesized by a condensation reaction of either 2-(1-methylhydrazinyl)pyridine or 2-(1-methylhydrazinyl)quinoline with an aldehyde (picolinaldehyde, 1H-pyrrole-2-carbaldehyde, 2-mercaptobenzaldehyde, 2-aminobenzaldehyde) have been reacted with palladium(II) and platinum(II) salts and precursor complexes to yield nine new metal complexes. These planar complexes were investigated with respect to their capability to interact with guanine quadruplex DNA. Two sequences (H-telo, derived from the human telomeric sequence, and c-myc, representing an excerpt of the promoter region of the c-Myc oncogene) were investigated...
July 5, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28713155/hypoxia-induces-universal-but-differential-drug-resistance-and-impairs-anticancer-mechanisms-of-5-fluorouracil-in-hepatoma-cells
#17
Jing-Qiu Li, Xian Wu, Lu Gan, Xiang-Liang Yang, Ze-Hong Miao
Hepatocellular carcinoma (HCC) is one of the most refractory cancers. The mechanisms by which hypoxia further aggravates therapeutic responses of advanced HCC to anticancer drugs remain to be clarified. Here, we report that hypoxia (1% O2) caused 2.55-489.7-fold resistance to 6 anticancer drugs (sorafenib, 5-fluorouracil [5-FU], gemcitabine, cisplatin, adriamycin and 6-thioguanine) in 3 HCC cell lines (BEL-7402, HepG2 and SMMC-7721). Among the 6 drugs, sorafenib, the sole one approved for HCC therapy, inhibited proliferation with little influence from hypoxia and displayed the smallest variation among the 3 HCC cell lines tested...
July 17, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28711067/pa-6-inhibits-inward-rectifier-currents-carried-by-v93i-and-d172n-gain-of-function-kir2-1-channels-but-increases-channel-protein-expression
#18
Yuan Ji, Marlieke G Veldhuis, Jantien Zandvoort, Fee L Romunde, Marien J C Houtman, Karen Duran, Gijs van Haaften, Eva-Maria Zangerl-Plessl, Hiroki Takanari, Anna Stary-Weinzinger, Marcel A G van der Heyden
BACKGROUND: The inward rectifier potassium current IK1 contributes to a stable resting membrane potential and phase 3 repolarization of the cardiac action potential. KCNJ2 gain-of-function mutations V93I and D172N associate with increased IK1, short QT syndrome type 3 and congenital atrial fibrillation. Pentamidine-Analogue 6 (PA-6) is an efficient (IC50 = 14 nM with inside-out patch clamp methodology) and specific IK1 inhibitor that interacts with the cytoplasmic pore region of the KIR2...
July 15, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28708860/charged-amino-acid-variability-related-to-n-glyco-sylation-and-epitopes-in-a-h3n2-influenza-hem-agglutinin-and-neuraminidase
#19
Zhong-Zhou Huang, Liang Yu, Ping Huang, Li-Jun Liang, Qing Guo
BACKGROUND: The A/H3N2 influenza viruses circulated in humans have been shown to undergo antigenic drift, a process in which amino acid mutations result from nucleotide substitutions. There are few reports regarding the charged amino acid mutations. The purpose of this paper is to explore the relations between charged amino acids, N-glycosylation and epitopes in hemagglutinin (HA) and neuraminidase (NA). METHODS: A total of 700 HA genes (691 NA genes) of A/H3N2 viruses were chronologically analyzed for the mutational variants in amino acid features, N-glycosylation sites and epitopes since its emergence in 1968...
2017: PloS One
https://www.readbyqxmd.com/read/28705810/the-butyrophilin-3a1-intracellular-domain-undergoes-a-conformational-change-involving-the-juxtamembrane-region
#20
Khiem Nguyen, Jin Li, Robbins Puthenveetil, Xiaochen Lin, Michael M Poe, Chia-Hung Christine Hsiao, Olga Vinogradova, Andrew J Wiemer
Small isoprenoid diphosphates, such as (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate (HMBPP), are ligands of the internal domain of BTN3A1. Ligand binding in target cells promotes activation of Vγ9Vδ2 T cells. We demonstrate by small-angle X-ray scattering (SAXS) that HMBPP binding to the internal domain of BTN3A1 induces a conformational change in the position of the B30.2 domain relative to the juxtamembrane (JM) region. To better understand the molecular details of this conformational rearrangement, NMR spectroscopy was used to discover that the JM region interacts with HMBPP, specifically at the diphosphate...
July 13, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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