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https://www.readbyqxmd.com/read/27843711/iron-overload-induced-death-of-osteoblasts-in-vitro-involvement-of-the-mitochondrial-apoptotic-pathway
#1
Qing Tian, Shilei Wu, Zhipeng Dai, Jingjing Yang, Jin Zheng, Qixin Zheng, Yong Liu
BACKGROUND: Iron overload is recognized as a new pathogenfor osteoporosis. Various studies demonstrated that iron overload could induce apoptosis in osteoblasts and osteoporosis in vivo. However, the exact molecular mechanisms involved in the iron overload-mediated induction of apoptosis in osteoblasts has not been explored. PURPOSE: In this study, we attempted to determine whether the mitochondrial apoptotic pathway is involved in iron-induced osteoblastic cell death and to investigate the beneficial effect of N-acetyl-cysteine (NAC) in iron-induced cytotoxicity...
2016: PeerJ
https://www.readbyqxmd.com/read/27522712/-the-effects-of-cysplatin-on-human-adipose-tissue-derived-mesenchymal-stromal-cells-under-different-oxygen-levels
#2
V Rylova, L B Buravkova, B D Zhivotovky
OBJECTIVE: To evaluate the damaging effects of cisplatin on MMSCs from adipose tissue in a phase of active proliferation and the state of the monolayer, this was exposed at standard (20%) and reduced to 1% and 5% level of oxygen. METHODS: The effect of cisplatin was detected on monolayer cultures in the active growth phase after 2 passages. Profile surface markers of MMSC was determined by flow cytometry. MMSCs viability after incubation with cisplatin was detected by the number of apoptotic and necrotic cells using ANNEXIN V-FITC--PI Kit (Immunotech, France)...
2016: Vestnik Rossiĭskoĭ Akademii Meditsinskikh Nauk
https://www.readbyqxmd.com/read/27503846/stemcell-keep%C3%A2-is-effective-for-cryopreservation-of-human-embryonic-stem-cells-by-vitrification
#3
Akemi Ota, Kazuaki Matsumura, Jun-Jae Lee, Shoichiro Sumi, Soung Hyu Hyon
Safe and stable cryopreservation is critical for research involving human embryonic stem (hES) cells. Dimethyl sulfoxide (DMSO) is a popular cryoprotective agent; however, its cytotoxicity cannot be ignored. Thus, there is a need for an alternate cryoprotectant. We reported previously that a novel cryopreservation reagent, StemCell Keep™ (SCK) was effective for cryopreserving human induced pluripotent stem (hiPS) cells by vitrification. Because hES and hiPS cells are not identical, the current study examined the use of SCK on hES cells...
August 5, 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27404861/neural-stem-cell-trailblazer-stemcells-folds
#4
Eric Smalley
No abstract text is available yet for this article.
July 12, 2016: Nature Biotechnology
https://www.readbyqxmd.com/read/27392222/10-questions-approaches-to-research-funding
#5
(no author information available yet)
We asked four stem cell scientists who recently started their labs or expanded their research programs to share their insights and approaches to obtaining funding. We present highlights from their interview responses here.
July 7, 2016: Cell Stem Cell
https://www.readbyqxmd.com/read/27223437/establishment-of-a-multimarker-qpcr-panel-for-the-molecular-characterization-of-circulating-tumor-cells-in-blood-samples-of-metastatic-breast-cancer-patients-during-the-course-of-palliative-treatment
#6
Maren Bredemeier, Philippos Edimiris, Mitra Tewes, Pawel Mach, Bahriye Aktas, Doreen Schellbach, Jenny Wagner, Rainer Kimmig, Sabine Kasimir-Bauer
BACKGROUND: Circulating tumor cells (CTC) are discussed to be an ideal surrogate marker for individualized treatment in metastatic breast cancer (MBC) since metastatic tissue is often difficult to obtain for repeated analysis. We established a nine gene qPCR panel to characterize the heterogeneous CTC population in MBC patients including epithelial CTC, their receptors (EPCAM, ERBB2, ERBB3, EGFR) CTC in Epithelial-Mesenchymal-Transition [(EMT); PIK3CA, AKT2), stem cell-like CTC (ALDH1) as well as resistant CTC (ERCC1, AURKA] to identify individual therapeutic targets...
July 5, 2016: Oncotarget
https://www.readbyqxmd.com/read/27179111/changes-in-expression-of-genes-representing-key-biologic-processes-after-neoadjuvant-chemotherapy-in-breast-cancer-and-prognostic-implications-in-residual-disease
#7
Marie Klintman, Richard Buus, Maggie Chon U Cheang, Amna Sheri, Ian E Smith, Mitch Dowsett
PURPOSE: The primary aim was to derive evidence for or against the clinical importance of several biologic processes in patients treated with neoadjuvant chemotherapy (NAC) by assessing expression of selected genes with prior implications in prognosis or treatment resistance. The secondary aim was to determine the prognostic impact in residual disease of the genes' expression. EXPERIMENTAL DESIGN: Expression levels of 24 genes were quantified by NanoString nCounter on formalin-fixed paraffin-embedded residual tumors from 126 patients treated with NAC and 56 paired presurgical biopsies...
May 15, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/27075977/multicompartmental-hollow-fiber-based-bioreactors-for-dynamic-three-dimensional-perfusion-culture
#8
Eva Schmelzer, Jörg C Gerlach
The creation of larger-scale three-dimensional tissue constructs depends on proper medium mass and gas exchange, as well as removal of metabolites, which cannot be achieved in conventional static two-dimensional petri dish culture. In cultures of tissue-density this problem can be addressed by decentral perfusion through artificial micro-capillaries. While the static medium exchange in petri dishes leads to metabolite peaks, perfusion culture provides a dynamic medium supply, thereby preventing non-physiological peaks...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/26949089/zinc-finger-protein-521-overexpression-increased-transcript-levels-of-fndc5-in-mouse-embryonic-stem-cells
#9
Motahere-Sadat Hashemi, Abbas Kiani Esfahani, Maryam Peymani, Alireza Shoaraye Nejati, Kamran Ghaedi, Mohammad Hossein Nasr-Esfahani, Hossein Baharvand
Zinc finger protein 521 is highly expressed in brain, neural stem cells and early progenitors of the human hematopoietic cells. Zfp521 triggers the cascade of neurogenesis in mouse embryonic stem cells through inducing expression of the early neuroectodermal genes Sox1, Sox3 and Pax6. Fndc5, a precursor of Irisin has inducing effects on the expression level of brain derived neurotrophic factor in hippocampus. Therefore, it is most likely that Fndc5 may play an important role in neural differentiation. To exhibit whether the expression of this protein is under regulation with Zfp521, we overexpressed Zfp521 in a stable transformants of mESCs expressing EGFP under control of Fndc5 promoter...
March 2016: Journal of Biosciences
https://www.readbyqxmd.com/read/26849303/10-questions-clinical-outlook-for-ipscs
#10
(no author information available yet)
In an interview, we recently asked four leaders in the field to share their insights with us on the clinical outlook for hiPSCs. We present highlights from their responses here.
February 4, 2016: Cell Stem Cell
https://www.readbyqxmd.com/read/26240275/autocrine-signaling-by-wnt-5a-deregulates-chemotaxis-of-leukemic-cells-and-predicts-clinical-outcome-in-chronic-lymphocytic-leukemia
#11
Pavlina Janovska, Lucie Poppova, Karla Plevova, Hana Plesingerova, Martin Behal, Marketa Kaucka, Petra Ovesna, Michaela Hlozkova, Marek Borsky, Olga Stehlikova, Yvona Brychtova, Michael Doubek, Michaela Machalova, Sivasubramanian Baskar, Alois Kozubik, Sarka Pospisilova, Sarka Pavlova, Vitezslav Bryja
PURPOSE: ROR1, a receptor in the noncanonical Wnt/planar cell polarity (PCP) pathway, is upregulated in malignant B cells of chronic lymphocytic leukemia (CLL) patients. It has been shown that the Wnt/PCP pathway drives pathogenesis of CLL, but which factors activate the ROR1 and PCP pathway in CLL cells remains unclear. EXPERIMENTAL DESIGN: B lymphocytes from the peripheral blood of CLL patients were negatively separated using RosetteSep (StemCell) and gradient density centrifugation...
January 15, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/25629291/correlation-of-nadh-fluorescence-lifetime-and-oxidative-phosphorylation-metabolism-in-the-osteogenic-differentiation-of-human-mesenchymal-stem-cell
#12
Han-Wen Guo, Jia-Sin Yu, Shu-Han Hsu, Yau-Huei Wei, Oscar K Lee, Chen-Yuan Dong, Hsing-Wen Wang
Reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime has been broadly used as a metabolic indicator for stem cell imaging. However, the direct relationship between NADH fluorescence lifetime and metabolic pathway and activity remains to be clarified. In this study, we measured the NADH fluorescence lifetime of human mesenchymal stem cells (hMSCs) as well as the metabolic indictors, such as adenosine triphosphate (ATP) level, oxygen consumption, and lactate release, up to 4 weeks under normal osteogenic differentiation and oxidative phosphorylation-attenuated/inhibited differentiation by oligomycin A (OA) treatment...
January 2015: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/25341333/-construction-of-beta-catenin-mirna-expressing-vectors-and-test-of-silencing-effect-in-adipose-derived-stem-cells-from-sprague-dawley-rats
#13
Hai-Sen Huang, Shuang Liu, Mei Yu, Xiao-Xin Luo, Wei-Hua Guo, Wei-Dong Tian
UNLABELLED: OBEJECTIVE: To construct miRNA-expressing plasmid vector for interfering the expression of beta-catenin in adipose-derived stem cells from Sprague-Dawley rats. METHODS: The double strands oligonucleotides of miR-expressing cassette were ligated into plasmid pSWH to generate pSWH-miR. Then the PCR product of enhanced green fluorescence protein (EGFP) open reading frame (ORF) fragment was inserted into BamH I and Xbo I digested pSWH-miR to generate pSWH-EGFP-miR...
September 2014: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/25270877/codex-a-next-generation-sequencing-experiment-database-for-the-haematopoietic-and-embryonic-stem-cell-communities
#14
Manuel Sánchez-Castillo, David Ruau, Adam C Wilkinson, Felicia S L Ng, Rebecca Hannah, Evangelia Diamanti, Patrick Lombard, Nicola K Wilson, Berthold Gottgens
CODEX (http://codex.stemcells.cam.ac.uk/) is a user-friendly database for the direct access and interrogation of publicly available next-generation sequencing (NGS) data, specifically aimed at experimental biologists. In an era of multi-centre genomic dataset generation, CODEX provides a single database where these samples are collected, uniformly processed and vetted. The main drive of CODEX is to provide the wider scientific community with instant access to high-quality NGS data, which, irrespective of the publishing laboratory, is directly comparable...
January 2015: Nucleic Acids Research
https://www.readbyqxmd.com/read/25098365/tissue-engineering-for-rotator-cuff-repair-an-evidence-based-systematic-review
#15
REVIEW
Nicola Maffulli, Umile Giuseppe Longo, Mattia Loppini, Alessandra Berton, Filippo Spiezia, Vincenzo Denaro
The purpose of this systematic review was to address the treatment of rotator cuff tears by applying tissue engineering approaches to improve tendon healing, specifically platelet rich plasma (PRP) augmentation, stem cells, and scaffolds. Our systematic search was performed using the combination of the following terms: "rotator cuff", "shoulder", "PRP", "platelet rich plasma", "stemcells", "scaffold", "growth factors", and "tissue engineering". No level I or II studies were found on the use of scaffolds and stem cells for rotator cuff repair...
2012: Stem Cells International
https://www.readbyqxmd.com/read/24974357/effect-of-stem-cell-application-on-asherman-syndrome-an-experimental-rat-model
#16
Sevtap Kilic, Beril Yuksel, F Pinarli, A Albayrak, B Boztok, T Delibasi
PURPOSE: We evaluate the effect of stem cells to induce endometrial proliferation and angiogenesis on Asherman Syndrome (AS). METHODS: The experimental study was performed in stemcell research laboratory. Forty Wistar-Albino rats were divided according to groups. In group1 (n = 10) to establish the model; trichloroacetic acid was injected to right uterine horn. Two weeks later, intrauterine synechia was confirmed. In group2 (n = 10), 2 weeks later, 2 × 106 mesenchymal stem cells (MSC) were injected into right uterine horn followed by three intraperitoneal injections of MSCs...
August 2014: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/24886724/low-dose-insulin-like-growth-factor-binding-proteins-1-and-2-and-angiopoietin-like-protein-3-coordinately-stimulate-ex-vivo-expansion-of-human-umbilical-cord-blood-hematopoietic-stem-cells-as-assayed-in-nod-scid-gamma-null-mice
#17
Xiubo Fan, Florence Ph Gay, Francesca Wi Lim, Justina Ml Ang, Pat Py Chu, Sudipto Bari, William Yk Hwang
INTRODUCTION: Insulin-like growth factors (IGFs), IGF binding proteins (IGFBPs) and angiopoietin-like proteins (ANGPTLs) can enhance the ex vivo expansion of hematopoietic stem cells (HSCs) when used with a standard cytokine cocktail of stem cell factor (SCF), thrombopoietin (TPO) and FLT3 ligand (FL). In order to determine the optimal dose and combination of IGFs, IGFBPs and ANGPTLs, serial dilution and full permutation of IGFBP1, IGFBP2, IGF2 and ANGPTL3 were applied on a cryopreserved umbilical cord blood mononuclear cell (UCB-MNC) ex vivo expansion system...
May 30, 2014: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/24510488/production-of-monoclonal-antibodies
#18
Wayne M Yokoyama, Michelle Christensen, Gary Dos Santos, Diane Miller, Jason Ho, Tao Wu, Michael Dziegelewski, Francisca A Neethling
This unit describes the production of monoclonal antibodies beginning with immunization, cell fusion, and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell-HY from StemCell Technologies)...
2013: Current Protocols in Immunology
https://www.readbyqxmd.com/read/24219905/pre-conditioning-mesenchymal-stromal-cell-spheroids-for-immunomodulatory-paracrine-factor-secretion
#19
Joshua A Zimmermann, Todd C McDevitt
BACKGROUND AIMS: Mesenchymal stromal cells (MSCs) exhibit the inherent potential to regulate multiple signaling pathways and cell types that contribute to the pathogenesis of inflammatory and immune diseases. However, more recent studies have suggested that the secretion of immunomodulatory factors by MSCs can be enhanced by three-dimensional aggregation or pro-inflammatory cytokine treatment. METHODS: Human MSC spheroids were formed by forced aggregation into agarose micro-wells and subsequently cultured in either minimal essential medium alpha supplemented with fetal bovine serum or serum-free, defined MesenCult-XF medium (STEMCELL Technologies, Vancouver, Canada)...
March 2014: Cytotherapy
https://www.readbyqxmd.com/read/24106724/myogenic-differentiation-of-mesenchymal-stem-cells-in-a-newly-developed-neurotised-av-loop-model
#20
Franz F Bitto, Dorothee Klumpp, Claudia Lange, Anja M Boos, Andreas Arkudas, Oliver Bleiziffer, Raymund E Horch, Ulrich Kneser, Justus P Beier
Generation of axially vascularized muscle tissue constitutes a promising new approach to restoration of damaged muscle tissue. Mesenchymal stemcells (MSC), with their ability to be expanded to large cell numbers without losing their differentiation capacity into the myogenic lineage, could offer a promising cell source to generate neomuscle tissue. In vitro experiments showed that cocultures of primary myoblasts and MSC undergo myogenic differentiation by stimulation with bFGF and dexamethasone. A newly developed AV-Loop model with neurotization was established in this study...
2013: BioMed Research International
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