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Connective tissue pelvic organ prolapse

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https://www.readbyqxmd.com/read/27855713/microrna-29-facilitates-transplantation-of-bone-marrow-derived-mesenchymal-stem-cells-to-alleviate-pelvic-floor-dysfunction-by-repressing-elastin
#1
Minfei Jin, Yuelin Wu, Jun Wang, Weiping Ye, Lei Wang, Peipei Yin, Wei Liu, Chenhao Pan, Xiaolin Hua
BACKGROUND: Pelvic floor dysfunction (PFD) is a condition affecting many women worldwide, with symptoms including stress urinary incontinence (SUI) and pelvic organ prolapse (POP). We have previously demonstrated stable elastin-expressing bone marrow-derived mesenchymal stem cells (BMSCs) attenuated PFD in rats, and aim to further study the effect of microRNA-29a-3p regulation on elastin expression and efficacy of BMSC transplantation therapy. METHODS: We inhibited endogenous microRNA-29a-3p in BMSCs and investigated its effect on elastin expression by RT-PCR and Western blot...
November 17, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27636223/transcriptional-regulation-of-connective-tissue-metabolism-genes-in-women-with-pelvic-organ-prolapse
#2
Ali Borazjani, Nathan Kow, Samantha Harris, Beri Ridgeway, Margot S Damaser
OBJECTIVE: The aim of this study was to compare differences in expressions and relationships between key genes involved in extracellular matrix metabolism and tissue cellularity in women with and without pelvic organ prolapse (POP). METHODS: A total of 80 biopsies (anterior cuff, posterior cuff, and/or leading edge) were obtained from 30 women: n = 10 premenopausal without POP (controls), n = 10 premenopausal with POP, and n = 10 postmenopausal with POP. Quantitative reverse-transcriptase polymerase chain reaction was used to assess gene expression of bone morphogenetic protein 1 (BMP1), collagen types I (COL1) and III (COL3), relaxin family peptide receptor 1 (RXFP1), matrix metallopeptidase 2, and TIMP metallopeptidase inhibitors 2 and 3...
September 15, 2016: Female Pelvic Medicine & Reconstructive Surgery
https://www.readbyqxmd.com/read/27632406/risk-for-exfoliation-syndrome-in-women-with-pelvic-organ-prolapse-a-utah-project-on-exfoliation-syndrome-upexs-study
#3
Barbara M Wirostko, Karen Curtin, Robert Ritch, Samuel Thomas, Kristina Allen-Brady, Ken R Smith, Gregory S Hageman, R Rand Allingham
Importance: Exfoliation syndrome, an inherited systemic disorder of elastin and extracellular matrix (ECM), is associated with the LOXL1 gene locus. Pelvic organ prolapse is a common connective tissue disorder that affects women. The presence of an association between exfoliation syndrome (exfoliation of the lens capsule) and pelvic organ prolapse was investigated as part of the Utah Project on Exfoliation Syndrome (UPEXS). Objective: To examine the association between exfoliation syndrome and pelvic organ prolapse using the Utah Population Database, a comprehensive resource linked to medical records...
September 15, 2016: JAMA Ophthalmology
https://www.readbyqxmd.com/read/27588175/matrix-metalloproteinase-3-gene-promoter-polymorphisms-a-potential-risk-factor-for-pelvic-organ-prolapse
#4
Charalampos Karachalios, Panagiotis Bakas, Georgios Kaparos, Styliani Demeridou, Ilias Liapis, Charalampos Grigoriadis, Aggelos Liapis
Pelvic organ prolapse (POP) is a common multifactorial condition. Matrix metalloproteinases (MMPs) are enzymes capable of breaking down various connective tissue elements. Single-nucleotide polymorphisms (SNPs) in regulatory areas of MMP-encoding genes can alter their transcription rate, and therefore the possible effect on pelvic floor supporting structures. The insertion of an adenine (A) base in the promoter of the MMP-3 gene at position -1612/-1617 produces a sequence of six adenines (6A), whereas the other allele has five (5A)...
September 2016: Biomedical Reports
https://www.readbyqxmd.com/read/27517338/what-s-new-in-the-functional-anatomy-of-pelvic-organ-prolapse
#5
John O L DeLancey
PURPOSE OF REVIEW: Provide an evidence-based review of pelvic floor functional anatomy related to pelvic organ prolapse. RECENT FINDINGS: Pelvic organ support depends on interactions between the levator ani muscle and pelvic connective tissues. Muscle failure exposes the vaginal wall to a pressure differential producing abnormal tension on the attachments of the pelvic organs to the pelvic sidewall. Birth-induced injury to the pubococcygeal portion of the levator ani muscle is seen in 55% of women with prolapse and 16% of women with normal support...
October 2016: Current Opinion in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/27124299/pelvic-organ-support-in-animals-with-partial-loss-of-fibulin-5-in-the-vaginal-wall
#6
Kathleen Chin, Cecilia Wieslander, Haolin Shi, Sunil Balgobin, T Ignacio Montoya, Hiromi Yanagisawa, R Ann Word
Compromise of elastic fiber integrity in connective tissues of the pelvic floor is most likely acquired through aging, childbirth-associated injury, and genetic susceptibility. Mouse models of pelvic organ prolapse demonstrate systemic deficiencies in proteins that affect elastogenesis. Prolapse, however, does not occur until several months after birth and is thereby acquired with age or after parturition. To determine the impact of compromised levels of fibulin-5 (Fbln5) during adulthood on pelvic organ support after parturition and elastase-induced injury, tissue-specific conditional knockout (cKO) mice were generated in which doxycycline (dox) treatment results in deletion of Fbln5 in cells that utilize the smooth muscle α actin promoter-driven reverse tetracycline transactivator and tetracycline responsive element-Cre recombinase (i...
2016: PloS One
https://www.readbyqxmd.com/read/27091643/comparative-histology-of-mouse-rat-and-human-pelvic-ligaments
#7
Ritsuko Iwanaga, David J Orlicky, Jameson Arnett, Marsha K Guess, K Joseph Hurt, Kathleen A Connell
INTRODUCTION AND HYPOTHESIS: The uterosacral (USL) and cardinal ligaments (CL) provide support to the uterus and pelvic organs, and the round ligaments (RL) maintain their position in the pelvis. In women with pelvic organ prolapse (POP), the connective tissue, smooth muscle, vasculature, and innervation of the pelvic support structures are altered. Rodents are commonly used animal models for POP research. However, the pelvic ligaments have not been defined in these animals. In this study, we hypothesized that the gross anatomy and histological composition of pelvic ligaments in rodents and humans are similar...
April 18, 2016: International Urogynecology Journal
https://www.readbyqxmd.com/read/27048404/transplantation-of-bone-marrow-derived-mesenchymal-stem-cells-expressing-elastin-alleviates-pelvic-floor-dysfunction
#8
Minfei Jin, Ying Chen, Yun Zhou, Yan Mei, Wei Liu, Chenhao Pan, Xiaolin Hua
BACKGROUND: Pelvic floor dysfunction (PFD) is a group of clinical conditions including stress urinary incontinence (SUI) and pelvic organ prolapse (POP). The abnormality of collagen and elastin metabolism in pelvic connective tissues is implicated in SUI and POP. METHODS: To reconstitute the connective tissues with normal distribution of collagen and elastin, we transduced elastin to bone marrow-derived mesenchymal stem cells (BMSC). Elastin-expressing BMSCs were then differentiated to fibroblasts using bFGF, which produced collagen and elastin...
2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/26820994/nanofibrous-biomimetic-mesh-can-be-used-for-pelvic-reconstructive-surgery-a-randomized-study
#9
Jing Ding, Mou Deng, Xiao-Chen Song, Chun Chen, Kui-Lin Lai, Guo-Shuai Wang, Yu-Yu Yuan, Tao Xu, Lan Zhu
BACKGROUND: Implantation of nonabsorbable polypropylene (PP) mesh in the vagina is the main surgical treatment for pelvic organ prolapse (POP); however, clinical outcomes remain controversial and far from satisfactory. In particular, reducing the exposure or erosion of vaginal implants to obtain improved functional reconstruction is challenging. There is an urgent need for the development of new materials and/or products for POP treatment. A nanofibrous biomimetic mesh was recently developed to address this issue...
August 2016: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/26732347/a-biofidelic-computational-model-of-the-female-pelvic-system-to-understand-effect-of-bladder-fill-and-progressive-vaginal-tissue-stiffening-due-to-prolapse-on-anterior-vaginal-wall
#10
Arnab Chanda, Vinu Unnikrishnan, Holly E Richter, Mark E Lockhart
Treatment of Anterior Vaginal Prolapse (AVP), suffered by over 500,000 women in the U.S, is a challenge in urogynecology due to the poorly understood mechanics of AVP. Recently, computational modeling combined with finite element method (FEM) have been used to model AVP through the study of pelvic floor muscle and connective tissue impairments on the AVW. Also the effects of pelvic organ displacements on the AVW were studied numerically. In our current work, an MRI-based full scale biofidelic computational model of the female pelvic system comprised of the urinary bladder, vaginal canal, and the uterus was developed and a novel FEM framework was employed to simulate vaginal tissue stiffening and also bladder filling due to expansion for the first time...
January 5, 2016: International Journal for Numerical Methods in Biomedical Engineering
https://www.readbyqxmd.com/read/26656625/identifying-distinct-nanoscopic-features-of-native-collagen-fibrils-towards-early-diagnosis-of-pelvic-organ-prolapse
#11
Taeyoung Kim, Indumathi Sridharan, Yin Ma, Bofan Zhu, Naiwei Chi, William Kobak, Jacob Rotmensch, Jay D Schieber, Rong Wang
UNLABELLED: Pelvic organ prolapse (POP) is characterized by weakening of the connective tissues and loss of support for the pelvic organs. Collagen is the predominant, load-bearing protein within pelvic floor connective tissues. In this study, we examined the nanoscopic structures and biomechanics of native collagen fibrils in surgical, vaginal wall connective tissues from healthy women and POP patients. Compared to controls, collagen fibrils in POP samples were bulkier, more uneven in width and stiffer with aberrant D-period...
April 2016: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/26448995/-management-of-vaginal-cones-in-the-treatment-of-pelvic-floor-rehabilitation
#12
Maialen Onandia Garate, Ana María Luces Lago, Lucía Mosquera Pan, Eva Tizón Bouza
The pelvic floor (PF) is a sheet of muscles and other tissues that support the pelvic organs in their physiological positions. Throughout women's lives, these structures can become weak or be injured by events such as pregnancy, childbirth, surgery, overweight or constipation. PF dysfunction includes a group of disorders causing urinary incontinence, as well as genital prolapse or pelvic pain, and can significantly deteriorate women's quality of life. Vaginal cones (VC) represent a non-pharmacological, economical, safe and non-invasive method for the treatment of PF dysfunction; they allow the patient to increase the physiological consciousness of the musculature of the PF while promoting an increase in the muscle tone...
July 2015: Revista de Enfermería
https://www.readbyqxmd.com/read/26337427/cystocele-and-functional-anatomy-of-the-pelvic-floor-review-and-update-of-the-various-theories
#13
Géry Lamblin, Emmanuel Delorme, Michel Cosson, Chrystèle Rubod
INTRODUCTION AND HYPOTHESIS: We updated anatomic theories of pelvic organ support to determine pathophysiology in various forms of cystocele. METHODS: PubMed/MEDLINE, ScienceDirect, Cochrane Library, and Web of Science databases were searched using the terms pelvic floor, cystocele, anatomy, connective tissue, endopelvic fascia, and pelvic mobility. We retrieved 612 articles, of which 61 matched our topic and thus were selected. Anatomic structures of bladder support and their roles in cystocele onset were determined on the international anatomic classification; the various anatomic theories of pelvic organ support were reviewed and a synthesis was made of theories of cystocele pathophysiology...
September 2016: International Urogynecology Journal
https://www.readbyqxmd.com/read/26205621/muscle-fragments-on-a-scaffold-in-rats-a-potential-regenerative-strategy-in-urogynecology
#14
Hanna Jangö, Søren Gräs, Lise Christensen, Gunnar Lose
INTRODUCTION AND HYPOTHESIS: The use of permanent synthetic meshes to improve the outcome of pelvic organ prolapse (POP) repair causes frequent and serious complications. The use of the synthetic, biodegradable scaffold methoxypolyethyleneglycol-polylactic-co-glycolic acid (MPEG-PLGA) seeded with autologous muscle fiber fragments (MFF), as an adjunct to native tissue POP repair, is a potential new alternative. METHODS: A rat abdominal wall model of native repair was used with six animals in each of three groups: native repair, native repair + MPEG-PLGA, and native repair + MPEG-PLGA + MFF...
December 2015: International Urogynecology Journal
https://www.readbyqxmd.com/read/25930307/are-demographic-and-clinical-characteristics-of-women-presenting-with-advanced-pelvic-organ-prolapse-different-compared-with-women-presenting-in-earlier-stages-374
#15
Alain A Mukwege, Sherif A El-Nashar, Deborah Rhodes, Sean C Dowdy, Emanuel C Trabuco
OBJECTIVE: To compare demographic and clinical characteristics between women who had surgery for pelvic organ prolapse at an advanced compared with early stage. METHODS: This is an observational study including women who underwent surgical treatment for stage 4 and stage 2 apical pelvic organ prolapse at Mayo Clinic Rochester between January 1, 2002, and June 19, 2012. The associations between clinical and demographic characteristics and surgery for advanced compared with early pelvic organ prolapse were evaluated...
May 2015: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/25891043/expression-of-fibulin-5-in-the-skin-of-patients-with-rectal-prolapse
#16
H M Joshi, M P Gosselink, E A Smyth, R Hompes, C Cunningham, I Lindsey, J Urban, O M Jones
AIM: Components of connective tissue other than collagen have been found to be involved in patients with rectal prolapse. The organization of elastic fibres differs between controls and subsets of patients with rectal prolapse, and their importance for maintaining the structural and functional integrity of the pelvic floor has been demonstrated in transgenic mice, with animals which have a null mutation in fibulin-5 (Fbln5(i/i)) developing prolapse. This study aimed to compare fibulin-5 expression in the skin of patients with and without rectal prolapse...
November 2015: Colorectal Disease: the Official Journal of the Association of Coloproctology of Great Britain and Ireland
https://www.readbyqxmd.com/read/25740571/collagen-structure-deterioration-in-the-skin-of-patients-with-pelvic-organ-prolapse-determined-by-atomic-force-microscopy
#17
Svetlana L Kotova, Peter S Timashev, Anna E Guller, Anatoly B Shekhter, Pavel I Misurkin, Victor N Bagratashvili, Anna B Solovieva
We used atomic force microscopy (AFM) to diagnose pathological changes in the extracellular matrix (ECM) of skin connective tissue in patients with pelvic organ prolapse (POP). POP is a common condition affecting women that considerably decreases the patients' quality of life. Deviations from normal morphology of the skin ECM from patients with POP occur including packing and arrangement of individual collagen fibers and arrangement of collagen fibrils. The nanoindentation study revealed significant deterioration of the mechanical properties of collagen fibril bundles in the skin of POP patients as compared with the skin of healthy subjects...
April 2015: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/25630974/microrna-30d-and-microrna-181a-regulate-hoxa11-expression-in-the-uterosacral-ligaments-and-are-overexpressed-in-pelvic-organ-prolapse
#18
Myung Jae Jeon, Eun Jae Kim, Maria Lee, Hoguen Kim, Jong Rak Choi, Hee Dong Chae, Yeo Jung Moon, Sei Kwang Kim, Sang Wook Bai
The balanced turnover of collagen is necessary to maintain the mechanical strength of pelvic supportive connective tissues. Homeobox (HOX) A11 is a key transcriptional factor that controls collagen metabolism and homoeostasis in the uterosacral ligaments (USLs), and the deficient HOXA11 signalling may contribute to alterations in the biochemical strength of the USLs, leading to pelvic organ prolapse (POP). However, it is unknown how HOXA11 transcripts are regulated in the USLs. In this study, we found that microRNA (miRNA)-30d and 181a were overexpressed in women with POP, and their expression was inversely correlated with HOXA11 mRNA levels...
February 2015: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/25608269/histological-study-on-the-effects-of-microablative-fractional-co2-laser-on-atrophic-vaginal-tissue-an-ex-vivo-study
#19
Stefano Salvatore, Umberto Leone Roberti Maggiore, Stavros Athanasiou, Massimo Origoni, Massimo Candiani, Alberto Calligaro, Nicola Zerbinati
OBJECTIVE: Microablative fractional CO2 laser has been proven to determine tissue remodeling with neoformation of collagen and elastic fibers on atrophic skin. The aim of our study is to evaluate the effects of microablative fractional CO2 laser on postmenopausal women with vulvovaginal atrophy using an ex vivo model. METHODS: This is a prospective ex vivo cohort trial. Consecutive postmenopausal women with vulvovaginal atrophy managed with pelvic organ prolapse surgical operation were enrolled...
August 2015: Menopause: the Journal of the North American Menopause Society
https://www.readbyqxmd.com/read/25421935/label-free-three-dimensional-multiphoton-microscopy-of-the-connective-tissue-in-the-anterior-vaginal-wall
#20
COMPARATIVE STUDY
Michal Sikora, David Scheiner, Cornelia Betschart, Daniele Perucchini, José María Mateos, Anthony di Natale, Daniel Fink, Caroline Maake
INTRODUCTION AND HYPOTHESIS: Multiphoton microscopy (MPM) is a nonlinear, high-resolution laser scanning technique and a powerful approach for analyzing the spatial architecture within tissues. To demonstrate the potential of this technique for studying the extracellular matrix of the pelvic organs, we aimed to establish protocols for the detection of collagen and elastin in the vagina and to compare the MPM density of these fibers to fibers detected using standard histological methods...
May 2015: International Urogynecology Journal
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