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Il 6

Keizo Kohno, Satomi Koya-Miyata, Akira Harashima, Toshio Ariyasu, Shimpei Ushio
NK-4 is the main component of the antiallergic drug Lumin, which has been in popular usage since the early 1950s. In this study, we examined whether NK-4 exerts a regulatory effect on the activation and effector function of Th2 cells. NK-4 inhibited IL-4 production by anti-CD3ε mAb-stimulated BALB/c mouse spleen cells, whereas NK-4 had little effect on IFN-γ production. IL-4 and IL-5 secretion by anti-CD3ε mAb- or antigen-stimulated Th2 cells (D10.G4.1) was abrogated by NK-4 without affecting cell numbers, whereas IFN-γ secretion by activated Th1 cells was unchanged...
2018: PloS One
Qianli Huang, Xuezhong Li, Tarek A Elkhooly, Xujie Liu, Ranran Zhang, Hong Wu, Qingling Feng, Yong Liu
The implant materials with both osteogenic and anti-bacterial properties are promising for orthopedic and dental applications. Moreover, the inflammatory response induced by biomaterials has been recently recognized as one of the critical factors in determining implantation fate. A new generation of implant materials should have modulatory effects on the local inflammatory environment such that it favors osteogenesis and osteointegration instead of being bio-inert. In this study, the micro-arc oxidation (MAO) technique was employed to fabricate Cu-containing ceramic coatings on titanium substrates...
June 19, 2018: Colloids and Surfaces. B, Biointerfaces
Shuangjiao Deng, Hongfei Wang, Heng Fan, Lijuan Zhang, Jianli Hu, Qing Tang, Zhexing Shou, Xingxing Liu, Dongmei Zuo, Jia Yang, Meng Xu, Qianyun Chen, Yalan Dong, Zhen Nan, Hui Wu, Yujin Liu
Our study was to explore the potential role of miRNA-200b in modulating tumorigenesis in the model of ulcerative colitis-related colorectal cancer (UCRCC) and, further, to decipher the underlying mechanisms associated with this effect. In this study, we examined a greater number of polyps or adenomas, a higher grade of epithelial dysplasia accompanied with a decrease in survival ratio in azoxymethane (AOM)/dextran sulfate sodium (DSS) model mice compared to mice treated with over-expressed miRNA-200b. Surprisingly, enforced miRNA-200b expression significantly suppressed AOM/DSS-induced up-regulation of oncologic markers including β-catenin and CD133...
June 19, 2018: International Immunopharmacology
Zhiwen Wang, Yu Huang, Yuan Li, Bei Wang, Yishan Lu, Liqun Xia, Jufen Tang, JiChang Jian
Nile tilapia (Oreochromis niloticus) is a pivotal economic fish that has been plagued by Streptococcus agalactiae infections for many years. Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a crucial adaptor molecule of the interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily, which can trigger downstream signaling cascades involved in innate immunity. In this study, the full-length cDNA of TRAF6 was cloned from O. niloticus (named On-TRAF6), which has an open reading frame of 1716 bp, and encode a polypeptide of 571 amino acids...
June 19, 2018: Fish & Shellfish Immunology
Monica M Gaudier-Diaz, Adam H Haines, Ning Zhang, A Courtney DeVries
Social isolation presents a risk factor and worsens outcome to cerebrovascular diseases; however, the underlying mechanisms remain underspecified. This study examines the effect of social environment on microglial reactivity after global cerebral ischemia, to test the hypothesis that social isolation leads to greater microglial responses. Adult female and male mice were pair-housed or socially isolated for one week prior to cardiac arrest/cardiopulmonary resuscitation (CA/CPR) or the sham procedure, and following either 2 or 24 h of reperfusion, microglia samples were enriched and analyzed for gene expression...
June 19, 2018: Physiology & Behavior
Dan-Dan Zhu, Yun-Zhi Wang, Chen Zou, Xin-Ping She, Zhi Zheng
OBJECTIVE: Uric acid has been proposed as an independent risk factor of diabetic retinopathy. Although Notch signaling was reported to be affected in the presence of high concentrations of uric acid or glucose, the underlying mechanisms of hyperuricemia through the Notch signaling pathway to promote the development of diabetic retinopathy remain unknown. METHODS: We incubated human retinal endothelial cells (HRECs) with high glucose, high uric acid and high glucose plus high glucose respectively and evaluated the apoptosis rate in different treated cells by Tunel staining...
June 19, 2018: Biochemical and Biophysical Research Communications
Samara L Potter, Norma M Quintanilla, Danielle K Johnston, Bindi Naik-Mathuria, Rajkumar Venkatramani
Angiomatoid fibrous histiocytoma (AFH) is a rare soft tissue tumor that has been associated with EWSR1-CREB1 gene fusion. Outcome in patients with unresectable distant metastases is generally fatal. Interleukin-6 (IL-6) secretion has been described in tumors with EWSR1-CREB1 fusion, and may promote tumor growth due to autocrine stimulation. Tocilizumab is an IL-6 receptor antibody that has potential benefit as a targeted therapy in refractory neoplasms with IL-6 secretion. We describe a child with metastatic AFH with EWSR1-CREB1 fusion and elevated IL-6 whose disease progressed during treatment with traditional chemotherapeutic agents, but improved after targeted therapy with tocilizumab...
June 22, 2018: Pediatric Blood & Cancer
Yang Liu, YingNan Zhang, Qingfeng Liang, Chao Yan, Li Wang, Jing Zhang, ZhiQiang Pan
BACKGROUND: In current non-human primate models, full-thickness porcine grafts could not achieve long-term survival without using potent systemic immunosuppressors. Moreover, the thickness disparity in xeno-PKP proved to be hard to manipulate and may cause several complications which also could prevent the grafts from long survival. Considering the advantages of Descemet's stripping automated endothelial keratoplasty (DSAEK) derived from its ultrathin graft and lower rejection rate, we hypothesize xeno-DSAEK may overcome these imperfections in xeno-PKP...
June 22, 2018: Xenotransplantation
Hanchao Gao, Qing Zhang, Jicheng Chen, David K C Cooper, Hidetaka Hara, Pengfei Chen, Ling Wei, Yanli Zhao, Jia Xu, Zesong Li, Zhiming Cai, Shaodong Luan, Lisha Mou
Whether porcine cytokines are induced after pig-to-primate xenotransplantation and activate human cells remains unknown. First, we investigated the regulation of porcine IL-6, IFN-γ, IL-1β, and TNF-α in xenotransplantation using an in vitro model in which porcine aortic endothelial cells (PAECs) and porcine peripheral blood mononuclear cells (PBMCs) were stimulated with human serum. Downstream cytokines/chemokines were monitored. Pro-inflammatory cytokines (IL-6, IFN-γ, and IL-1β) and chemokines (IL-8, MCP-1, and CXCL2) were upregulated in the both cell types...
June 22, 2018: Xenotransplantation
Jizhou Zhang, Qing Li, Qiqi Shao, Jingling Song, Bin Zhou, Peng Shu
Panax notoginseng saponin (PNS) constitutes the major effective components of Panax notoginseng, which is widely used to treat microcirculatory disturbance associated diseases. In this study, we designed to investigate the effect of PNS on the treatment of pulmonary fibrosis (PF) and further explored its mechanism. A total of 40 healthy Japanese White rabbits were randomly divided into five groups (control group; PF model group; PNS prevention group; PNS treatment group; and western medicine [prednisone acetate] treatment group)...
June 22, 2018: Journal of Cellular Biochemistry
Dinesh Thummuri, Lalita Guntuku, Veerabhadra Swamy Challa, Ravinder Naik Ramavat, Vegi Ganga Modi Naidu
Osteoporosis is a major debilitating cause of fractures and decreases the quality of life in elderly patients. Bone homeostasis is maintained by bone forming osteoblasts and bone resorpting osteoclasts. Substantial evidences have shown that targeting osteoclasts using natural products is a promising strategy for the treatment of osteoporosis. In the current study, we investigated the osteoprotective effect of Abietic acid (AA) in in vitro and in vivo models of osteolysis. In vitro experiments demonstrated that, AA suppressed receptor activator of nuclear factor-kappa B ligand (RANKL)-induced osteoclastogenesis and F-actin ring formation in a concentration dependent manner...
June 22, 2018: Journal of Cellular Physiology
Takehiro Sugawara, Sakura Onoue, Hiroaki Takimoto, Kazuyoshi Kawahara
Lauroyltransferase gene (lpxL), Myristoyltransferase gene (lpxM) and palmitoyltransferase gene (crcA) of Escherichia coli BL21 were independently disrupted by the insertional mutations. The knockout mutant of two transferase genes (lpxL and crcA) produced lipid A with no lauric and palmitic acids and only little amount of myristic acid. The mutant was susceptible to polymyxin B, but showed comparable growth with wild-type strain at 30°C. The palmitoyltransferase gene from E. coli (crcA) or Salmonella (pagP) was amplified by PCR, cloned in pUC119, and transferred into the double-knockout mutant by transformation...
June 22, 2018: Microbiology and Immunology
Ju-Hye Yang, Esther Lee, BoHyoung Lee, Won-Kyung Cho, Jin Yeul Ma, Kwang-Il Park
Artemisia apiacea Hance is a traditional herbal medicine used for treating eczema and jaundice in Eastern Asia including China, Korea, and Japan. However, the biological and pharmacological actions of Artemisia apiacea Hance in atopic dermatitis (AD) are not fully understood. An ethanolic extract of Artemisia apiacea Hance (EAH) was tested in vitro and in vivo to investigate its anti-inflammatory activity and anti-atopic dermatitis effects. The results showed that EAH dose-dependence inhibited production of regulated on activation, normal T-cell expressed and secreted (RANTES), interleukin (IL)-6, IL-8, and thymus and activation-regulated chemokine (TARC)...
June 22, 2018: Nutrients
Sun-Hee Ahn, Jin-Kyung Lee, Nam Doo Kim, Seok-Ho Kim, Sunwoo Lee, Seunggon Jung, Kee-Oh Chay, Tae-Hoon Lee
Interleukin-1β (IL-1β) is a prominent pro-inflammatory cytokine that is implicated in a variety of autoimmune diseases and plays an important role in host defense against infections. IL-1β activity increases with its increasing binding capacity to IL-1 receptors (IL-1Rs). Thus, numerous studies have targeted the discovery of molecules modulating the interactions between IL-1β and IL-1R1. We have conducted an IL-1R1 structure-based virtual screening to identify small molecules that could alter IL-1β activity, using in silico computational analysis...
June 22, 2018: International Journal of Molecular Sciences
Swati Bagde, Vinod Singh
BACKGROUND: The Cellular immunity plays an important role in amoebic infections, whereas humoral immune responses have little influence on the outcome of the disease. Chronic infestation with E. histolytica, a common protozoan parasite, has been associated with autoimmune phenomena, including the appearance of antibodies to colonic epithelial cells. The mechanism whereby the protozoan parasite E. histolytica causes suppression of the immune response to allow it to survive in humans is unclear...
June 21, 2018: Recent Patents on Inflammation & Allergy Drug Discovery
Sha Zhang, Jie Li, Pei Xie, Ting Zang, Haibin Shen, Guochun Cao, Ying Zhu, Zhenggang Yue, Zhe Li
Chronic inflammation is a major driving factor for the development of colitis-associated cancer (CAC). It is extensively acknowledged that patients who have long-standing inflammation bowel disease (IBD) are at high risk for developing CAC. However, the metabolic alteration by which chronic intestinal inflammation promotes colorectal cancer is unclear. In the present study, we constructed dextran sulfate sodium (DSS)-induced chronic colitis mouse model to uncover possible alterations in the metabolism indexes...
June 22, 2018: Rejuvenation Research
Yvette N Lamb, Emma D Deeks
Sarilumab (Kevzara® ), a monoclonal antibody against the interleukin-6 (IL-6) receptor, is approved in various countries, including the USA, those of the EU, and Japan, as a subcutaneous treatment administered every 2 weeks for moderately to severely active rheumatoid arthritis (RA) in adults who have responded inadequately to, or are intolerant of, one or more DMARDs. In placebo-controlled trials, sarilumab improved the signs and symptoms of RA, as well as physical function and health-related quality-of-life (HR-QOL), when administered in combination with conventional synthetic DMARD (csDMARD) therapy in patients with an inadequate response to methotrexate or an inadequate response to, or intolerance of, at least one tumour necrosis factor (TNF) inhibitor; benefits were sustained over ≤ 3 years' therapy in an open-label extension...
June 2018: Drugs
Ninad Varkhede, Nita Patel, William Chang, Kenneth Ruterbories, M Laird Forrest
PURPOSE: To investigate influence of inflammation on metabolism and pharmacokinetics (PK) of midazolam (MDZ) and construct a semi-physiologically based pharmacokinetic (PBPK) model to predict PK in mice with inflammatory disease. METHODS: Glucose-6-phosphate isomerase (GPI)-mediated inflammation was used as a preclinical model of arthritis in DBA/1 mice. CYP3A substrate MDZ was selected to study changes in metabolism and PK during the inflammation. The semi-PBPK model was constructed using mouse physiological parameters, liver microsome metabolism, and healthy animal PK data...
June 21, 2018: Pharmaceutical Research
Verena Liebers, Benjamin Kendzia, Heike Stubel, Gerda Borowitzki, Vitali Gering, Christian Monsé, Olaf Hagemeyer, Rolf Merget, Thomas Brüning, Monika Raulf
The whole-blood assay (WBA) with human fresh blood may provide insight into the features of an individual's innate immunity. To assess this, ex vivo cytokine release is measured after stimulation of whole blood with various stimuli, for instance, endotoxin in vitro. The aim of the present study was to evaluate WBA reproducibility with fresh blood using different calculation models. The blood was collected from 16 healthy volunteers on 6 different days. Ex vivo stimulation was performed in each individual's blood sample for 22 h, using different endotoxin concentrations...
June 22, 2018: Advances in Experimental Medicine and Biology
Satomi Kasashima, Atsuhiro Kawashima, Fuminori Kasashima, Masamitsu Endo, Yasushi Matsumoto, Kengo Kawakami
Immunoglobulin (IgG) 4-related diseases (IgG4-RDs) are fibro-inflammatory conditions characterized by tumorous swelling and serum IgG4 levels. Intrapelvic IgG4-RD has been subclassified according to the localization site and aortic shape as IgG4-related aortic aneurysms (IgG4-AAs), periaortitis (IgG4-PA), and retroperitoneal fibrosis (IgG4-RF). The IgG4-AA pathogenesis would involve interleukin (IL)-6 upregulation, and Th2-predominant and Treg-activated immune conditions. We characterized the features of intrapelvic IgG4-RD lesions, including presence of vascular lesions...
June 21, 2018: Heart and Vessels
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