keyword
MENU ▼
Read by QxMD icon Read
search

Optogenetic

keyword
https://www.readbyqxmd.com/read/28728024/origins-of-cell-type-specific-olfactory-processing-in-the-drosophila-mushroom-body-circuit
#1
Kengo Inada, Yoshiko Tsuchimoto, Hokto Kazama
How cell-type-specific physiological properties shape neuronal functions in a circuit remains poorly understood. We addressed this issue in the Drosophila mushroom body (MB), a higher olfactory circuit, where neurons belonging to distinct glomeruli in the antennal lobe feed excitation to three types of intrinsic neurons, α/β, α'/β', and γ Kenyon cells (KCs). Two-photon optogenetics and intracellular recording revealed that whereas glomerular inputs add similarly in all KCs, spikes were generated most readily in α'/β' KCs...
July 19, 2017: Neuron
https://www.readbyqxmd.com/read/28726577/will-optogenetics-be-used-to-treat-chronic-pain-patients
#2
Hélène Beaudry, Ihab Daou, Alfredo Ribeiro-da-Silva, Philippe Séguéla
Chronic pain affects a third of the population and current treatments produce limited relief and severe side effects. An alternative strategy to decrease pain would be to directly modulate somatosensory pathways using optogenetics. Optogenetics involves the use of genetically encoded and optically active proteins, namely opsins, to control neuronal circuits. In preclinical animal models, optical silencing of peripheral nociceptors has been shown to alleviate both inflammatory and neuropathic pain. An opsin-based gene therapy to treat chronic pain patients is not ready yet, but encouraging advances have been made in optical and viral technology...
July 20, 2017: Pain Management
https://www.readbyqxmd.com/read/28721358/utah-optrode-array-customization-using-stereotactic-brain-atlases-and-3-d-cad-modeling-for-optogenetic-neocortical-interrogation-in-small-rodents-and-nonhuman-primates
#3
Ronald W Boutte, Sam Merlin, Guy Yona, Brandon Griffiths, Alessandra Angelucci, Itamar Kahn, Shy Shoham, Steve Blair
As the optogenetic field expands, the need for precise targeting of neocortical circuits only grows more crucial. This work demonstrates a technique for using Solidworks(®) computer-aided design (CAD) and readily available stereotactic brain atlases to create a three-dimensional (3-D) model of the dorsal region of area visual cortex 4 (V4D) of the macaque monkey (Macaca fascicularis) visual cortex. The 3-D CAD model of the brain was used to customize an [Formula: see text] Utah optrode array (UOA) after it was determined that a high-density ([Formula: see text]) UOA caused extensive damage to marmoset (Callithrix jacchus) primary visual cortex as assessed by electrophysiological recording of spiking activity through a 1...
October 2017: Neurophotonics
https://www.readbyqxmd.com/read/28720792/optical-read-out-of-neural-activity-in-mammalian-peripheral-axons-calcium-signaling-at-nodes-of-ranvier
#4
Arjun K Fontaine, Emily A Gibson, John H Caldwell, Richard F Weir
Current neural interface technologies have serious limitations for advanced prosthetic and therapeutic applications due primarily to their lack of specificity in neural communication. An optogenetic approach has the potential to provide single cell/axon resolution in a minimally invasive manner by optical interrogation of light-sensitive reporters and actuators. Given the aim of reading neural activity in the peripheral nervous system, this work has investigated an activity-dependent signaling mechanism in the peripheral nerve...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28720664/dynein-efficiently-navigates-the-dendritic-cytoskeleton-to-drive-the-retrograde-trafficking-of-bdnf-trkb-signaling-endosomes
#5
Swathi Ayloo, Pedro Guedes-Dias, Amy E Ghiretti, Erika L F Holzbaur
The efficient transport of cargoes within axons and dendrites is critical for neuronal function. While we have a basic understanding of axonal transport, much less is known about transport in dendrites. We employed an optogenetic approach to recruit motor proteins to cargo in real time within axons or dendrites in hippocampal neurons. Kinesin-1, a robust axonal motor, moves cargo less efficiently in dendrites. In contrast, cytoplasmic dynein efficiently navigates both axons and dendrites; in both compartments, dynamic microtubule plus-ends enhance dynein-dependent transport...
July 18, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28720520/neural-systems-mediating-the-inhibition-of-cocaine-seeking-behaviors
#6
REVIEW
Victória A Ewald, Ryan T LaLumiere
Over the past decades, research has targeted the neurobiology regulating cocaine-seeking behaviors, largely in the hopes of identifying potential targets for the treatment of cocaine addiction. Although much of this work has focused on those systems driving cocaine seeking, recently, studies examining the inhibition of cocaine-related behaviors have made significant progress in uncovering the neural systems that attenuate cocaine seeking. Such systems include the infralimbic cortex, nucleus accumbens shell, and hypothalamus...
July 15, 2017: Pharmacology, Biochemistry, and Behavior
https://www.readbyqxmd.com/read/28719603/the-%C3%A2-100-lab-a-3d-printable-open-source-platform-for-fluorescence-microscopy-optogenetics-and-accurate-temperature-control-during-behaviour-of-zebrafish-drosophila-and-caenorhabditis-elegans
#7
Andre Maia Chagas, Lucia L Prieto-Godino, Aristides B Arrenberg, Tom Baden
Small, genetically tractable species such as larval zebrafish, Drosophila, or Caenorhabditis elegans have become key model organisms in modern neuroscience. In addition to their low maintenance costs and easy sharing of strains across labs, one key appeal is the possibility to monitor single or groups of animals in a behavioural arena while controlling the activity of select neurons using optogenetic or thermogenetic tools. However, the purchase of a commercial solution for these types of experiments, including an appropriate camera system as well as a controlled behavioural arena, can be costly...
July 2017: PLoS Biology
https://www.readbyqxmd.com/read/28715701/synthetic-biological-approaches-to-optogenetically-control-cell-signaling
#8
REVIEW
Katja Kolar, Wilfried Weber
Precise spatial and temporal control of cellular processes is in life sciences a highly sought-after capability. In the recent years, this goal has become progressively achievable through the field of optogenetics, which utilizes light as a non-invasive means to control genetically encoded light-responsive proteins. The latest optogenetic systems, such as those for control of subcellular localization or cellular decision-making and tissue morphogenesis provide us with insights to gain a deeper understanding of the cellular inner workings...
July 14, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28714952/a-circuit-based-mechanism-underlying-familiarity-signaling-and-the-preference-for-novelty
#9
Susanna Molas, Rubing Zhao-Shea, Liwang Liu, Steven R DeGroot, Paul D Gardner, Andrew R Tapper
Novelty preference (NP) is an evolutionarily conserved, essential survival mechanism often dysregulated in neuropsychiatric disorders. NP is mediated by a motivational dopamine signal that increases in response to novel stimuli, thereby driving exploration. However, the mechanism by which once-novel stimuli transition to familiar stimuli is unknown. Here we describe a neuroanatomical substrate for familiarity signaling, the interpeduncular nucleus (IPN) of the midbrain, which is activated as novel stimuli become familiar with multiple exposures...
July 17, 2017: Nature Neuroscience
https://www.readbyqxmd.com/read/28712566/left-habenular-activity-attenuates-fear-responses-in-larval-zebrafish
#10
Erik R Duboué, Elim Hong, Kiara C Eldred, Marnie E Halpern
Fear responses are defensive states that ensure survival of an organism in the presence of a threat. Perception of an aversive cue causes changes in behavior and physiology, such as freezing and elevated cortisol, followed by a return to the baseline state when the threat is evaded [1]. Neural systems that elicit fear behaviors include the amygdala, hippocampus, and medial prefrontal cortex. However, aside from a few examples, little is known about brain regions that promote recovery from an aversive event [2]...
July 8, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28708877/median-raphe-region-stimulation-alone-generates-remote-but-not-recent-fear-memory-traces
#11
Diána G Balázsfi, Dóra Zelena, Lívia Farkas, Kornél Demeter, István Barna, Csaba Cserép, Virág T Takács, Gábor Nyíri, Flóra Gölöncsér, Beáta Sperlágh, Tamás F Freund, József Haller
The median raphe region (MRR) is believed to control the fear circuitry indirectly, by influencing the encoding and retrieval of fear memories by amygdala, hippocampus and prefrontal cortex. Here we show that in addition to this established role, MRR stimulation may alone elicit the emergence of remote but not recent fear memories. We substituted electric shocks with optic stimulation of MRR in C57BL/6N male mice in an optogenetic conditioning paradigm and found that stimulations produced agitation, but not fear, during the conditioning trial...
2017: PloS One
https://www.readbyqxmd.com/read/28708765/distinct-behavioral-responses-evoked-by-selective-optogenetic-stimulation-of-the-major-trpv1-and-mrgd-subsets-of-c-fibers
#12
Hélène Beaudry, Ihab Daou, Ariel R Ase, Alfredo Ribeiro-da-Silva, Philippe Séguéla
Primary C-fiber nociceptors are broadly divided into peptidergic and non-peptidergic afferents. TRPV1 is a thermosensitive cation channel mainly localized in peptidergic nociceptors whereas MrgD is a sensory G protein-coupled receptor expressed in most non-peptidergic nociceptive afferents. TRPV1 and MrgD fibers have been reported to be primarily involved in thermal and mechanical nociception, respectively. Yet, their functional assessment in somatosensory transmission relied on ablation strategies that do not account for compensatory mechanisms...
July 12, 2017: Pain
https://www.readbyqxmd.com/read/28708260/effect-of-optogenetic-manipulation-of-accumbal-medium-spiny-neurons-expressing-dopamine-d2-receptors-in-cocaine-induced-behavioral-sensitization
#13
Byeong Jun Kang, Shelly Sooyun Song, Lei Wen, Ki-Pyo Hong, George J Augustine, Ja-Hyun Baik
Repetitive exposure to addictive drugs causes synaptic modification in the mesocorticolimbic dopamine (DA) system. Dopamine D1 receptors (D1R) or D2 receptors (D2R) expressed in the medium spiny neurons (MSNs) of the nucleus accumbens (NAc) play critical roles in the control of addictive behaviors. Optogenetic activation of D2R-expressing MSNs (D2R-MSNs) in the NAc previously demonstrated that these neurons play a key role in withdrawal-induced plasticity. Here, we examined the effect of optogenetic inhibition of D2R-MSNs in the NAc on cocaine-induced behavioral sensitization...
July 14, 2017: European Journal of Neuroscience
https://www.readbyqxmd.com/read/28708061/locus-coeruleus-to-basolateral-amygdala-noradrenergic-projections-promote-anxiety-like-behavior
#14
Jordan G McCall, Edward R Siuda, Dionnet L Bhatti, Lamley A Lawson, Zoe A McElligott, Garret D Stuber, Michael R Bruchas
Increased tonic activity of locus coeruleus noradrenergic (LC-NE) neurons induces anxiety-like and aversive behavior. While some information is known about the afferent circuitry that endogenously drives this neural activity and behavior, the downstream receptors and anatomical projections that mediate these acute risk aversive behavioral states via the LC-NE system remain unresolved. Here we use a combination of retrograde tracing, fast-scan cyclic voltammetry, electrophysiology, and in vivo optogenetics with localized pharmacology to identify neural substrates downstream of increased tonic LC-NE activity in mice...
July 14, 2017: ELife
https://www.readbyqxmd.com/read/28706064/history-of-winning-remodels-thalamo-pfc-circuit-to-reinforce-social-dominance
#15
Tingting Zhou, Hong Zhu, Zhengxiao Fan, Fei Wang, Yang Chen, Hexing Liang, Zhongfei Yang, Lu Zhang, Longnian Lin, Yang Zhan, Zheng Wang, Hailan Hu
Mental strength and history of winning play an important role in the determination of social dominance. However, the neural circuits mediating these intrinsic and extrinsic factors have remained unclear. Working in mice, we identified a dorsomedial prefrontal cortex (dmPFC) neural population showing "effort"-related firing during moment-to-moment competition in the dominance tube test. Activation or inhibition of the dmPFC induces instant winning or losing, respectively. In vivo optogenetic-based long-term potentiation and depression experiments establish that the mediodorsal thalamic input to the dmPFC mediates long-lasting changes in the social dominance status that are affected by history of winning...
July 14, 2017: Science
https://www.readbyqxmd.com/read/28701538/differential-expression-of-long-term-potentiation-among-identified-inhibitory-inputs-to-dopamine-neurons
#16
DeNard V Simmons, Alyssa K Petko, Carlos Antonio Paladini
The in vivo firing pattern of ventral tegmental area (VTA) dopamine neurons is controlled by GABA afferents originating primarily from the nucleus accumbens (NAc), rostromedial tegmental nucleus (RMTg), and from local GABA neurons within the VTA. Although different forms of plasticity have been observed from GABA inputs to VTA dopamine neurons - one dependent on cyclic GMP synthesis, the other dependent on adenylyl cyclase activation - it is unknown whether plasticity is differentially expressed in each. Using an optogenetic strategy, we show that identified inhibitory postsynaptic currents (IPSCs) from local VTA GABA neurons and NAc afferents exhibit a cyclic GMP-dependent long-term potentiation (LTP) that is capable of inhibiting the firing activity of dopamine neurons...
July 12, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28700627/network-analysis-of-exploratory-behaviors-of-mice-in-a-spatial-learning-and-memory-task
#17
Yusuke Suzuki, Itaru Imayoshi
The Barnes maze is one of the main behavioral tasks used to study spatial learning and memory. The Barnes maze is a task conducted on "dry land" in which animals try to escape from a brightly lit exposed circular open arena to a small dark escape box located under one of several holes at the periphery of the arena. In comparison with another classical spatial learning and memory task, the Morris water maze, the negative reinforcements that motivate animals in the Barnes maze are less severe and less stressful...
2017: PloS One
https://www.readbyqxmd.com/read/28700561/recent-patents-in-optogenetics-and-optochemistry
#18
(no author information available yet)
No abstract text is available yet for this article.
July 12, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28700536/fully-implantable-battery-free-wireless-optoelectronic-devices-for-spinal-optogenetics
#19
Vijay K Samineni, Jangyeol Yoon, Kaitlyn E Crawford, Yu Ra Jeong, Kajanna C McKenzie, Gunchul Shin, Zhaoqian Xie, Saranya S Sundaram, Yuhang Li, Min Young Yang, Jeonghyun Kim, Di Wu, Yeguang Xue, Xue Feng, Yonggang Huang, Aaron D Mickle, Anthony Banks, Jeong Sook Ha, Judith P Golden, John A Rogers, Robert W Gereau
The advent of optogenetic tools has allowed unprecedented insights into the organization of neuronal networks. Although recently developed technologies have enabled implementation of optogenetics for studies of brain function in freely moving, untethered animals, wireless powering and device durability pose challenges in studies of spinal cord circuits where dynamic, multidimensional motions against hard and soft surrounding tissues can lead to device degradation. We demonstrate here a fully implantable optoelectronic device powered by near-field wireless communication (NFC) technology, with a thin and flexible open-architecture that provides excellent mechanical durability, robust sealing against biofluid penetration and fidelity in wireless activation, thereby allowing for long-term optical stimulation of the spinal cord without constraint on the natural behaviors of the animals...
June 8, 2017: Pain
https://www.readbyqxmd.com/read/28698677/precise-spatio-temporal-control-of-rapid-optogenetic-cell-ablation-with-mem-killerred-in-zebrafish
#20
C Buckley, M T Carvalho, L K Young, S A Rider, C McFadden, C Berlage, R F Verdon, J M Taylor, J M Girkin, J J Mullins
The ability to kill individual or groups of cells in vivo is important for studying cellular processes and their physiological function. Cell-specific genetically encoded photosensitizing proteins, such as KillerRed, permit spatiotemporal optogenetic ablation with low-power laser light. We report dramatically improved resolution and speed of cell targeting in the zebrafish kidney through the use of a selective plane illumination microscope (SPIM). Furthermore, through the novel incorporation of a Bessel beam into the SPIM imaging arm, we were able to improve on targeting speed and precision...
July 11, 2017: Scientific Reports
keyword
keyword
5572
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"