keyword
MENU ▼
Read by QxMD icon Read
search

Optogenetic

keyword
https://www.readbyqxmd.com/read/28319307/green-light-induced-inactivation-of-receptor-signaling-using-cobalamin-binding-domains
#1
Stephanie Kainrath, Manuela Stadler, Eva Reichhart, Martin Distel, Harald Janovjak
Optogenetics and photopharmacology provide spatiotemporally precise control over protein interactions and protein function in cells and animals. Optogenetic methods that are sensitive to green light and can be used to break protein complexes are not broadly available but would enable multichromatic experiments with previously inaccessible biological targets. Herein, we repurposed cobalamin (vitamin B12) binding domains of bacterial CarH transcription factors for green-light-induced receptor dissociation. In cultured cells, we observed oligomerization-induced cell signaling for the fibroblast growth factor receptor 1 fused to cobalamin-binding domains in the dark that was rapidly eliminated upon illumination...
March 20, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28318784/kinetics-of-endogenous-camkii-required-for-synaptic-plasticity-revealed-by-optogenetic-kinase-inhibitor
#2
Hideji Murakoshi, Myung Eun Shin, Paula Parra-Bueno, Erzsebet M Szatmari, Akihiro C E Shibata, Ryohei Yasuda
Elucidating temporal windows of signaling activity required for synaptic and behavioral plasticity is crucial for understanding molecular mechanisms underlying these phenomena. Here, we developed photoactivatable autocamtide inhibitory peptide 2 (paAIP2), a genetically encoded, light-inducible inhibitor of CaMKII activity. The photoactivation of paAIP2 in neurons for 1-2 min during the induction of LTP and structural LTP (sLTP) of dendritic spines inhibited these forms of plasticity in hippocampal slices of rodents...
March 8, 2017: Neuron
https://www.readbyqxmd.com/read/28303092/synaptic-ensemble-underlying-the-selection-and-consolidation-of-neuronal-circuits-during-learning
#3
REVIEW
Yoshio Hoshiba, Takeyoshi Wada, Akiko Hayashi-Takagi
Memories are crucial to the cognitive essence of who we are as human beings. Accumulating evidence has suggested that memories are stored as a subset of neurons that probably fire together in the same ensemble. Such formation of cell ensembles must meet contradictory requirements of being plastic and responsive during learning, but also stable in order to maintain the memory. Although synaptic potentiation is presumed to be the cellular substrate for this process, the link between the two remains correlational...
2017: Frontiers in Neural Circuits
https://www.readbyqxmd.com/read/28303019/integration-of-optogenetics-with-complementary-methodologies-in-systems-neuroscience
#4
REVIEW
Christina K Kim, Avishek Adhikari, Karl Deisseroth
Modern optogenetics can be tuned to evoke activity that corresponds to naturally occurring local or global activity in timing, magnitude or individual-cell patterning. This outcome has been facilitated not only by the development of core features of optogenetics over the past 10 years (microbial-opsin variants, opsin-targeting strategies and light-targeting devices) but also by the recent integration of optogenetics with complementary technologies, spanning electrophysiology, activity imaging and anatomical methods for structural and molecular analysis...
March 17, 2017: Nature Reviews. Neuroscience
https://www.readbyqxmd.com/read/28302720/adjacent-channelrhodopsin-2-residues-within-transmembranes-2-and-7-regulate-cation-selectivity-and-distribution-of-the-two-open-states
#5
Ryan Richards, Robert E Dempski
Channelrhodopsin-2 (ChR2) is a light-activated channel that can conduct cations of multiple valencies down the electrochemical gradient. Under continuous light exposure, ChR2 transitions from a high conducting open state (O1) to a low conducting open state (O2) with differing ion selectivity. The molecular basis for the O1 to O2 transition and how ChR2 modulates selectivity between states is currently unresolved. To this end, we used steered molecular dynamics, electrophysiology, and kinetic modeling to identify residues that contribute to gating and selectivity in discrete open states...
March 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28302718/a-unique-choanoflagellate-enzyme-rhodopsin-with-cyclic-nucleotide-phosphodiesterase-activity
#6
Kazuho Yoshida, Satoshi P Tsunoda, Leonid S Brown, Hideki Kandori
Photoactivated adenylyl cyclase (PAC) and guanylyl cyclase rhodopsin (Rh-GC) increase the concentrations of intracellular cyclic nucleotides upon illumination, serving as promising second-generation tools in optogenetics. To broaden arsenal of such tools, it is desirable to have light-activatable enzymes which can decrease cyclic nucleotide concentrations in cells. Here, we report on an unusual microbial rhodopsin which may be able to meet the demand. It is found in the choanoflagellate Salpingoeca rosetta and contains a C-terminal cyclic nucleotide phosphodiesterase (PDE) domain...
March 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28301770/imaging-and-optically-manipulating-neuronal-ensembles
#7
Luis Carrillo-Reid, Weijian Yang, Jae-Eun Kang Miller, Darcy S Peterka, Rafael Yuste
The neural code that relates the firing of neurons to the generation of behavior and mental states must be implemented by spatiotemporal patterns of activity across neuronal populations. These patterns engage selective groups of neurons, called neuronal ensembles, which are emergent building blocks of neural circuits. We review optical and computational methods, based on two-photon calcium imaging and two-photon optogenetics, to detect, characterize, and manipulate neuronal ensembles in three dimensions. We review data using these methods in the mammalian cortex that demonstrate the existence of neuronal ensembles in the spontaneous and evoked cortical activity in vitro and in vivo...
March 15, 2017: Annual Review of Biophysics
https://www.readbyqxmd.com/read/28301742/microbial-rhodopsins-diversity-mechanisms-and-optogenetic-applications
#8
Elena G Govorunova, Oleg A Sineshchekov, Hai Li, John L Spudich
Microbial rhodopsins are a family of photoactive retinylidene proteins widespread throughout the microbial world. They are notable for their diversity of function, using variations of a shared seven-transmembrane helix design and similar photochemical reactions to carry out distinctly different light-driven energy and sensory transduction processes. Their study has contributed to our understanding of how evolution modifies protein scaffolds to create new protein chemistry, and their use as tools to control membrane potential with light is fundamental to optogenetics for research and clinical applications...
March 9, 2017: Annual Review of Biochemistry
https://www.readbyqxmd.com/read/28298301/in-vivo-optogenetic-activation-of-nav1-8-cutaneous-nociceptors-and-their-responses-to-natural-stimuli
#9
Megan L Uhelski, Daniel J Bruce, Philippe Seguela, George L Wilcox, Donald A Simone
Optogenetic methods utilizing expression of the light-sensitive protein channelrhodopsin-2 (ChR2) in neurons have enabled selective activation of specific subtypes or groups of neurons to determine their functions. Using a transgenic mouse model in which neurons natively expressing Nav1.8, a tetrodotoxin-resistant voltage-gated sodium channel, also express the light-gated channel ChR2, we have been able to determine the functional properties of Nav1.8-expressing cutaneous nociceptors of the glabrous skin in vivo...
March 15, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28297674/nanoscale-structural-plasticity-of-the-active-zone-matrix-modulates-presynaptic-function
#10
Oleg O Glebov, Rachel E Jackson, Christian M Winterflood, Dylan M Owen, Ellen A Barker, Patrick Doherty, Helge Ewers, Juan Burrone
The active zone (AZ) matrix of presynaptic terminals coordinates the recruitment of voltage-gated calcium channels (VGCCs) and synaptic vesicles to orchestrate neurotransmitter release. However, the spatial organization of the AZ and how it controls vesicle fusion remain poorly understood. Here, we employ super-resolution microscopy and ratiometric imaging to visualize the AZ structure on the nanoscale, revealing segregation between the AZ matrix, VGCCs, and putative release sites. Long-term blockade of neuronal activity leads to reversible AZ matrix unclustering and presynaptic actin depolymerization, allowing for enrichment of AZ machinery...
March 14, 2017: Cell Reports
https://www.readbyqxmd.com/read/28297663/disrupting-glutamate-co-transmission-does-not-affect-acquisition-of-conditioned-behavior-reinforced-by-dopamine-neuron-activation
#11
Dong V Wang, Thomas Viereckel, Vivien Zell, Åsa Konradsson-Geuken, Carl J Broker, Aleksandr Talishinsky, Ji Hoon Yoo, Melissa H Galinato, Emma Arvidsson, Andrew J Kesner, Thomas S Hnasko, Åsa Wallén-Mackenzie, Satoshi Ikemoto
Dopamine neurons in the ventral tegmental area (VTA) were previously found to express vesicular glutamate transporter 2 (VGLUT2) and to co-transmit glutamate in the ventral striatum (VStr). This capacity may play an important role in reinforcement learning. Although it is known that activation of the VTA-VStr dopamine system readily reinforces behavior, little is known about the role of glutamate co-transmission in such reinforcement. By combining electrode recording and optogenetics, we found that stimulation of VTA dopamine neurons in vivo evoked fast excitatory responses in many VStr neurons of adult mice...
March 14, 2017: Cell Reports
https://www.readbyqxmd.com/read/28297653/atomistic-study-of-intramolecular-interactions-in-the-closed-state-channelrhodopsin-chimera-c1c2
#12
Monika R VanGordon, Gaurav Gyawali, Steven W Rick, Susan B Rempe
Channelrhodopsins (ChR1 and ChR2) are light-activated ion channels that enable photomobility of microalgae from the genus Chlamydomonas. Despite common use of ChR2 in optogenetics for selective control and monitoring of individual neurons in living tissue, the protein structures remain unresolved. Instead, a crystal structure of the ChR chimera (C1C2), an engineered combination of helices I-V from ChR1, without its C-terminus, and helices VI-VII from ChR2, is used as a template for ChR2 structure prediction...
March 14, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28293892/light-regulated-protein-kinases-based-on-the-cry2-cib1-system
#13
Wignand W D Mühlhäuser, Maximilian Hörner, Wilfried Weber, Gerald Radziwill
Optogenetic approaches enable the control of biological processes in a time- and space-resolved manner. These light-based methods are noninvasive and by using light as sole activator minimize side effects in contrast to chemical inducers. Here, we provide a protocol for the targeted control of the activity of protein kinases in mammalian cells based on the photoreceptor cryptochrome 2 (CRY2) of Arabidopsis thaliana and its interaction partner CIB1. Blue light (450 nm)-induced binding of CRY2 to CIB1 allows the recruitment of a chimeric cytosolic protein kinase AKT1 to the plasma membrane accompanied with stimulation of its kinase activity...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28289214/photocyclic-behavior-of-rhodopsin-induced-by-an-atypical-isomerization-mechanism
#14
Sahil Gulati, Beata Jastrzebska, Surajit Banerjee, Ángel L Placeres, Przemyslaw Miszta, Songqi Gao, Karl Gunderson, Gregory P Tochtrop, Sławomir Filipek, Kota Katayama, Philip D Kiser, Muneto Mogi, Phoebe L Stewart, Krzysztof Palczewski
Vertebrate rhodopsin (Rh) contains 11-cis-retinal as a chromophore to convert light energy into visual signals. On absorption of light, 11-cis-retinal is isomerized to all-trans-retinal, constituting a one-way reaction that activates transducin (Gt) followed by chromophore release. Here we report that bovine Rh, regenerated instead with a six-carbon-ring retinal chromophore featuring a C(11)=C(12) double bond locked in its cis conformation (Rh6mr), employs an atypical isomerization mechanism by converting 11-cis to an 11,13-dicis configuration for prolonged Gt activation...
March 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28288335/how-to-control-cyclic-nucleotide-signaling-by-light
#15
REVIEW
Vera Jansen, Jan F Jikeli, Dagmar Wachten
Optogenetics allows to non-invasively manipulate cellular functions with spatio-temporal precision by combining genetic engineering with the control of protein function by light. Since the discovery of channelrhodopsin has pioneered the field, the optogenetic toolkit has been ever expanding and allows now not only to control neuronal activity by light, but rather a multitude of other cellular functions. One important application that has been established in recent years is the light-dependent control of second messenger signaling...
March 10, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28288126/manipulating-fear-associations-via-optogenetic-modulation-of-amygdala-inputs-to-prefrontal-cortex
#16
Oded Klavir, Matthias Prigge, Ayelet Sarel, Rony Paz, Ofer Yizhar
Fear-related disorders are thought to reflect strong and persistent fear memories. The basolateral amygdala (BLA) and the medial prefrontal cortex (mPFC) form strong reciprocal synaptic connections that play a key role in acquisition and extinction of fear memories. While synaptic contacts of BLA cells onto mPFC neurons are likely to play a crucial role in this process, the BLA connects with several additional nuclei within the fear circuit that could relay fear-associated information to the mPFC, and the contribution of direct monosynaptic BLA-mPFC inputs is not yet clear...
March 13, 2017: Nature Neuroscience
https://www.readbyqxmd.com/read/28288124/c1-neurons-mediate-a-stress-induced-anti-inflammatory-reflex-in-mice
#17
Chikara Abe, Tsuyoshi Inoue, Mabel A Inglis, Kenneth E Viar, Liping Huang, Hong Ye, Diane L Rosin, Ruth L Stornetta, Mark D Okusa, Patrice G Guyenet
C1 neurons, located in the medulla oblongata, mediate adaptive autonomic responses to physical stressors (for example, hypotension, hemorrhage and presence of lipopolysaccharides). We describe here a powerful anti-inflammatory effect of restraint stress, mediated by C1 neurons: protection against renal ischemia-reperfusion injury. Restraint stress or optogenetic C1 neuron (C1) stimulation (10 min) protected mice from ischemia-reperfusion injury (IRI). The protection was reproduced by injecting splenic T cells that had been preincubated with noradrenaline or splenocytes harvested from stressed mice...
March 13, 2017: Nature Neuroscience
https://www.readbyqxmd.com/read/28288123/optogenetic-control-with-a-photocleavable-protein-phocl
#18
Wei Zhang, Alexander W Lohman, Yevgeniya Zhuravlova, Xiaocen Lu, Matthew D Wiens, Hiofan Hoi, Sine Yaganoglu, Manuel A Mohr, Elena N Kitova, John S Klassen, Periklis Pantazis, Roger J Thompson, Robert E Campbell
To expand the range of experiments that are accessible with optogenetics, we developed a photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after violet-light-induced cleavage of a specific bond in the protein backbone. We demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase, Gal4 transcription factor, and a viral protease that in turn was used to activate opening of the large-pore ion channel Pannexin-1.
March 13, 2017: Nature Methods
https://www.readbyqxmd.com/read/28287570/investigating-motor-skill-learning-processes-with-a-robotic-manipulandum
#19
Susan Leemburg, Maiko Iijima, Olivier Lambercy, Lauriane Nallet-Khosrofian, Roger Gassert, Andreas Luft
Skilled reaching tasks are commonly used in studies of motor skill learning and motor function under healthy and pathological conditions, but can be time-intensive and ambiguous to quantify beyond simple success rates. Here, we describe the training procedure for reach-and-pull tasks with ETH Pattus, a robotic platform for automated forelimb reaching training that records pulling and hand rotation movements in rats. Kinematic quantification of the performed pulling attempts reveals the presence of distinct temporal profiles of movement parameters such as pulling velocity, spatial variability of the pulling trajectory, deviation from midline, as well as pulling success...
February 12, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28287505/design-and-implementation-of-an-automated-illuminating-culturing-and-sampling-system-for-microbial-optogenetic-applications
#20
Cameron J Stewart, Megan N McClean
Optogenetic systems utilize genetically-encoded proteins that change conformation in response to specific wavelengths of light to alter cellular processes. There is a need for culturing and measuring systems that incorporate programmed illumination and stimulation of optogenetic systems. We present a protocol for building and using a continuous culturing apparatus to illuminate microbial cells with programmed doses of light, and automatically acquire and analyze images of cells in the effluent. The operation of this apparatus as a chemostat allows the growth rate and the cellular environment to be tightly controlled...
February 19, 2017: Journal of Visualized Experiments: JoVE
keyword
keyword
5572
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"