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https://www.readbyqxmd.com/read/28805368/pyrophosphate-recognition-and-sensing-in-water-using-bis-zinc-ii-dipicolylamino-functionalized-peptides
#1
Katrina A Jolliffe
Phosphate oxoanions and phosphorylated biomolecules (such as nucleotides, lipids, and proteins) play key roles in a wide range of biological processes. The ability to selectively detect these ions in the presence of each other has numerous applications in biochemistry and biomedicine. However, receptors and sensors that can discriminate between polyphosphate species with high selectivity and in biologically relevant conditions are rare. In this Account, we show how peptides (both cyclic and linear) can be used to position two zinc(II)dipicolylamine [Zn(II)DPA] binding sites in an appropriate arrangement to provide selective binding of pyrophosphate (PPi) in the presence of other polyphosphate species, including ATP, and in complex media such as cell growth buffer...
August 14, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28804798/probing-biological-redox-chemistry-with-large-amplitude-fourier-transformed-ac-voltammetry
#2
Hope Adamson, Alan M Bond, Alison Parkin
Biological electron-exchange reactions are fundamental to life on earth. Redox reactions underpin respiration, photosynthesis, molecular biosynthesis, cell signalling and protein folding. Chemical, biomedical and future energy technology developments are also inspired by these natural electron transfer processes. Further developments in techniques and data analysis are required to gain a deeper understanding of the redox biochemistry processes that power Nature. This review outlines the new insights gained from developing Fourier transformed ac voltammetry as a tool for protein film electrochemistry...
August 14, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28803962/post-analytical-stability-of-23-common-chemistry-and-immunochemistry-analytes-in-incurred-samples
#3
Betina Klint Nielsen, Tina Frederiksen, Lennart Friis-Hansen, Pia Bükmann Larsen
BACKGROUND: Storage of blood samples after centrifugation, decapping and initial sampling allows ordering of additional blood tests. The pre-analytic stability of biochemistry and immunochemistry analytes has been studied in detail, but little is known about the post-analytical stability in incurred samples. METHODS: We examined the stability of 23 routine analytes on the Dimension Vista® (Siemens Healthineers, Denmark): 42-60 routine samples in lithium-heparin gel tubes (Vacutainer, BD, USA) were centrifuged at 3000×g for 10min...
August 10, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28803474/bioinspired-indole-prenylation-reactions-in-water
#4
Satomi Tanaka, Shinya Shiomi, Hayato Ishikawa
Isoprene units derived from dimethylallyl diphosphate (DMAPP) are an important motif in many natural products including terpenoids, carotenoids, steroids, and natural rubber. Understanding the chemical characteristics of DMAPP is an important topic in natural products chemistry, organic chemistry, and biochemistry. We have developed a direct bioinspired indole prenylation reaction using DMAPP or its equivalents as the electrophile in homogeneous aqueous acidic media in the absence of enzyme to provide prenylated indole products...
August 13, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28802861/contrasting-effects-of-vortioxetine-and-paroxetine-on-pineal-gland-biochemistry-in-a-tryptophan-depletion-model-of-depression-in-female-rats
#5
M Franklin, N Hlavacova, Y Li, I Bermudez, A Csanova, C Sanchez, D Jezova
We studied the effects of the multi-modal antidepressant, vortioxetine and the SSRI, paroxetine on pineal melatonin and monoamine synthesis in a sub-chronic tryptophan (TRP) depletion model of depression based on a low TRP diet. Female Sprague-Dawley rats were randomised to groups a) control, b) low TRP diet, c) low TRP diet+paroxetine and d) low TRP diet+vortioxetine. Vortioxetine was administered via the diet (0.76mg/kg of food weight) and paroxetine via drinking water (10mg/kg/day) for 14days. Both drugs resulted in SERT occupancies >90%...
August 9, 2017: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/28802807/acute-phase-response-in-babesia-canis-and-dirofilaria-immitis-co-infections-in-dogs
#6
Zorana Milanović, Anja Ilić, Jelena Francuski Andrić, Vladimir Radonjić, Anđelo Beletić, Milica Kovačević Filipović
Babesia canis and Dirofilaria immitis are emerging and geographically overlapping vector-borne pathogens in dogs. Infection with B. canis leads to acute-phase response (APR) that can be mild to severe and results in either non-complicated or complicated forms of the disease. The aim of this study was to determine whether acute B. canis infection is more severe in dogs with underlying asymptomatic D. immitis infection. Dogs of both sexes, different ages and breeds, with naturally occurring mono-infections with B...
August 2, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28802559/absence-of-subchronic-oral-toxicity-and-genotoxicity-of-rice-koji-with-aspergillus-terreus
#7
Jun-Won Yun, Seung-Hyun Kim, Yun-Soon Kim, Ji-Ran You, Eun-Young Cho, Jung-Hee Yoon, Euna Kwon, Sang Ju Lee, Seong Pil Kim, Jae Hoon Seo, Jae Pyung In, Jae Hun Ahn, Ja-June Jang, Jin-Sung Park, Jeong-Hwan Che, Byeong-Cheol Kang
Koji products have been considered as an effective fermented food consumed in East Asia with many health benefits. Particularly, rice koji with Aspergillus terreus (RAT) has been reported to be able to prevent hyperlipidemia and hepatic steatosis through regulating cholesterol synthesis. Despite its biological activities, there is a lack of comprehensive information to give an assurance of its safety. Therefore, the objective of this study was to perform a series of toxicological studies (repeated dose oral toxicity and genotoxicity) according to test guidelines published by the Organization for Economic Cooperation and Development...
August 9, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/28800423/relationship-of-executive-functioning-deficits-to-n-acetyl-aspartate-naa-and-gamma-aminobutyric-acid-gaba-in-youth-with-bipolar-disorder
#8
Rebekah S Huber, Douglas G Kondo, Xian-Feng Shi, Andrew P Prescot, Elaine Clark, Perry F Renshaw, Deborah A Yurgelun-Todd
BACKGROUND: Although cognitive deficits in bipolar disorder (BD) have been repeatedly observed, our understanding of these impairments at a mechanistic level remains limited. Few studies that investigated cognitive impairments in bipolar illness have examined the association with brain biochemistry. This pilot study utilized proton magnetic resonance spectroscopy ((1)H-MRS) to evaluate the relationship between neurocognitive performance and brain metabolites in youth with BD. METHODS: Thirty participants, twenty depressed BD participants and ten healthy comparison participants, ages 13-21, completed mood and executive function measures...
August 1, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/28798910/changes-in-acylglycerols-composition-quality-characteristics-and-in-vivo-effects-of-dietary-pumpkin-seed-oil-upon-thermal-oxidation
#9
Alam Zeb, Sultan Ahmad
This study was aimed to determine the acylglycerols composition, quality characteristics, and protective role of dietary pumpkin seed oil (PSO) in rabbits. PSO was thermally oxidized and analyzed for quality characteristics and acylglycerols composition using reversed phase high performance liquid chromatography with diode array detection (HPLC-DAD). Oxidized and un-oxidized oil samples were fed to the rabbits in different doses for 2 weeks. The changes in the serum biochemistry, hematology, and liver histology were studied...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/28798763/maturation-of-plastid-c-type-cytochromes
#10
REVIEW
Stéphane T Gabilly, Patrice P Hamel
Cytochromes c are hemoproteins, with the prosthetic group covalently linked to the apoprotein, which function as electron carriers. A class of cytochromes c is defined by a CXXCH heme-binding motif where the cysteines form thioether bonds with the vinyl groups of heme. Plastids are known to contain up to three cytochromes c. The membrane-bound cytochrome f and soluble cytochrome c6 operate in photosynthesis while the activity of soluble cytochrome c6A remains unknown. Conversion of apo- to holocytochrome c occurs in the thylakoid lumen and requires the independent transport of apocytochrome and heme across the thylakoid membrane followed by the stereospecific attachment of ferroheme via thioether linkages...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28797290/role-and-regulation-of-mkp-1-in-airway-inflammation
#11
REVIEW
Seyed M Moosavi, Pavan Prabhala, Alaina J Ammit
Mitogen-activated protein kinase (MAPK) phosphatase 1 (MKP-1) is a protein with anti-inflammatory properties and the archetypal member of the dual-specificity phosphatases (DUSPs) family that have emerged over the past decade as playing an instrumental role in the regulation of airway inflammation. Not only does MKP-1 serve a critical role as a negative feedback effector, controlling the extent and duration of pro-inflammatory MAPK signalling in airway cells, upregulation of this endogenous phosphatase has also emerged as being one of the key cellular mechanism responsible for the beneficial actions of clinically-used respiratory medicines, including β2-agonists, phosphodiesterase inhibitors and corticosteroids...
August 10, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28795915/where-teachers-are-few-documenting-available-faculty-in-five-tanzanian-medical-schools
#12
Charles A Mkony, Ephata E Kaaya, Alex J Goodell, Sarah B Macfarlane
Background Faced with one of the lowest physician-to-population ratios in the world, the Government of Tanzania is urging its medical schools to train more physicians. The annual number of medical students admitted across the country rose from 55 in the 1990s to 1,680 approved places for the 2015/16 academic year. These escalating numbers strain existing faculty. Objective To describe the availability of faculty in medical schools in Tanzania. Design We identified faculty lists published on the Internet by five Tanzanian medical schools for the 2011/12 academic year and analyzed the appointment status, rank, discipline, and qualifications of faculty members...
December 2016: Global Health Action
https://www.readbyqxmd.com/read/28795724/chronic-exposure-to-inorganic-mercury-induces-biochemical-and-morphological-changes-in-the-salivary-glands-of-rats
#13
W A B Aragão, N M M da Costa, N C F Fagundes, M C F Silva, S M Alves-Junior, J J V Pinheiro, L L Amado, M E Crespo-López, C S F Maia, R R Lima
Mercury exposure is considered to be a public health problem due to the generation of toxic effects on human health as a result of environmental and occupational conditions. The inorganic form of mercury (HgCl2) can cause several biological changes in cells and tissues through its cumulative toxic potential, but little has been experimentally proven about the effects of inorganic mercury on salivary glands, an important modulator organ of oral health. This study analyzes the effects of prolonged low dose exposure to HgCl2 on the salivary glands of rats...
August 10, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28795015/laboratory-reference-intervals-for-systolic-blood-pressure-rectal-temperature-haematology-biochemistry-and-venous-blood-gas-and-electrolytes-in-healthy-pet-rabbits
#14
Miguel Gallego
Prospective data from 86 healthy pet rabbits were evaluated to establish reference intervals for hematology, biochemistry, urinalysis, venous blood gas and electrolytes, rectal temperature and systolic blood pressure. Reference intervals for rectal temperature (37.4-39.6 ºC) and systolic blood pressure (75-134 mm/Hg) were previously unreported in pet rabbits. Differences by more than 30% with reference intervals present in the bibliography were observed in the blood biochemistry and urinalysis, being attributed to the variability in methodological factors with the present study...
2017: Open veterinary journal
https://www.readbyqxmd.com/read/28794850/dual-control-of-pituitary-thyroid-stimulating-hormone-secretion-by-thyroxine-and-triiodothyronine-in-athyreotic-patients
#15
Rudolf Hoermann, John E M Midgley, Johannes W Dietrich, Rolf Larisch
BACKGROUND: Patient responses to levothyroxine (LT4) monotherapy vary considerably. We sought to differentiate contributions of FT4 and FT3 in controlling pituitary thyroid stimulating hormone (TSH) secretion. METHODS: We retrospectively assessed the relationships between TSH and thyroid hormones in 319 patients with thyroid carcinoma through 2914 visits on various LT4 doses during follow-up for 5.5 years (median, IQR 4.2, 6.9). We also associated patient complaints with the relationships...
June 2017: Therapeutic Advances in Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28793853/equine-skeletal-muscle-adaptations-to-exercise-and-training-evidence-of-differential-regulation-of-autophagosomal-and-mitochondrial-components
#16
Kenneth Bryan, Beatrice A McGivney, Gabriella Farries, Paul A McGettigan, Charlotte L McGivney, Katie F Gough, David E MacHugh, Lisa M Katz, Emmeline W Hill
BACKGROUND: A single bout of exercise induces changes in gene expression in skeletal muscle. Regular exercise results in an adaptive response involving changes in muscle architecture and biochemistry, and is an effective way to manage and prevent common human diseases such as obesity, cardiovascular disorders and type II diabetes. However, the biomolecular mechanisms underlying such responses still need to be fully elucidated. Here we performed a transcriptome-wide analysis of skeletal muscle tissue in a large cohort of untrained Thoroughbred horses (n = 51) before and after a bout of high-intensity exercise and again after an extended period of training...
August 9, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28792798/cholecystopexy-and-pericardial-pseudocyst-removal-in-a-dog-with-a-congenital-peritoneopericardial-diaphragmatic-hernia
#17
Quentin Cabon, Eric Norman Carmel, Julien Cabassu
A 4 mo old spayed female golden retriever was presented with a peritoneopericardial diaphragmatic hernia (PPDH) that was diagnosed during neutering. Echocardiography revealed a fluid-filled structure and parts of the liver in the pericardial cavity. Computed tomography confirmed the existence of the PPDH and the herniation of the right medial liver lobe and the gallbladder. Cystic masses were observed in the pericardial and the peritoneal cavities, possibly communicating through the PPDH. A median laparotomy revealed a single lobulated cystic lesion extending into both the pericardial and peritoneal cavities through the PPDH...
August 9, 2017: Journal of the American Animal Hospital Association
https://www.readbyqxmd.com/read/28791640/study-of-egfr-signaling-endocytosis-by-site-directed-mutagenesis
#18
Qian Wang, Zhixiang Wang
As an essential tool in molecular biology and biochemistry, in vitro mutagenesis is important for mechanistic and functional studies of protein at the cellular level. Through site-directed substitution, insertion, or deletion of potential function residues, we may evaluate the specific biological role of proteins and understand the underlying mechanism. EGF ligand binding will activate EGFR and initiate signaling transduction for cell proliferation, differentiation, and survival; meanwhile, it will also induce the EGFR endocytosis for degradation to maintain cellular homeostasis...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28790918/accumulation-of-arsenic-speciation-and-in-vivo-toxicity-following-oral-administration-of-a-chinese-patent-medicine-xiao-er-zhi-bao-wan-in-rats
#19
Jiaoyang Luo, Xu Han, Xiaowen Dou, Lei Zhang, Shihai Yang, Meihua Yang
Realgar-containing traditional Chinese medicines such as Xiao-Er-Zhi-Bao-Wan (XEZBW), have been widely used for thousands of years. However, events associated with arsenic-induced ailments have increasingly become a public concern. To address the toxicity of XEZBW, we studied the histopathology and blood biochemistry of rats exposed to XEZBW using technology like high-performance liquid chromatography-inductively coupled mass spectrometry to determine arsenic speciation. Our results demonstrated that dimethylarsinic acid (DMA) increased from 18...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28782575/toxicological-evaluation-of-neoagarooligosaccharides-prepared-by-enzymatic-hydrolysis-of-agar
#20
Sun Joo Hong, Je-Hyeon Lee, Eun Joo Kim, Hea Jung Yang, Jae-Seon Park, Soon-Kwang Hong
Agar, a heterogeneous polymer of galactose, is the main component of the cell wall of marine red algae. It is well established as a safe, non-digestible carbohydrate in Oriental countries. Although neoagarooligosaccharides (NAOs) prepared by the hydrolysis of agar by β-agarase have been reported to exert various biological activities, the safety of these compounds has not been reported to date. For safety evaluation, NAOs containing mainly neoagarotetraose and neoagarohexaose were prepared from agar by enzymatic hydrolysis using β-agarase DagA from Streptomyces coelicolor...
August 3, 2017: Regulatory Toxicology and Pharmacology: RTP
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