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Giulia Cattaneo, Daniela Ubiali, Enrica Calleri, Marco Rabuffetti, Georg C Höfner, Klaus T Wanner, Marcela C De Moraes, Leonardo K B Martinelli, Diógenes Santiago Santos, Giovanna Speranza, Gabriella Massolini
Mycobacterium tuberculosis (Mtb) purine nucleoside phosphorylase (PNP, EC has been identified as a target for the development of specific inhibitors with potential antimycobacterial activity. We hereby described the development and validation of a new 96-well LC-ESI-MS/MS method to assess the inhibition activity of nucleoside analogues towards MtbPNP and the human PNP (HsPNP). Enzyme activity was determined by monitoring the phosphorolysis of inosine (Ino) to hypoxanthine (Hpx). The enzymatic assay (v = 0...
November 2, 2016: Analytica Chimica Acta
A L S Guimarães, S A Pereira, M N Diógenes, M A N Dode
The aim of this study was to evaluate the effect of adding a combination of insulin, transferrin and selenium (ITS) and l-ascorbic acid (AA) during in vitro maturation (IVM) and in vitro culture (IVC) on in vitro embryo production. To verify the effect of the supplements, cleavage and blastocyst rates, embryo size and total cell number were performed. Embryonic development data, embryo size categorization and kinetics of maturation were analyzed by chi-squared test, while the total cell number was analyzed by a Kruskal-Wallis test (P < 0...
October 17, 2016: Zygote: the Biology of Gametes and Early Embryos
Michael Ermisch, Anna Bucsics, Patricia Vella Bonanno, Francis Arickx, Alexander Bybau, Tomasz Bochenek, Marc van de Casteele, Eduardo Diogene, Jurij Fürst, Kristina Garuolienė, Martin van der Graaff, Jolanta Gulbinovič, Alan Haycox, Jan Jones, Roberta Joppi, Ott Laius, Irene Langner, Antony P Martin, Vanda Markovic-Pekovic, Laura McCullagh, Einar Magnusson, Ellen Nilsen, Gisbert Selke, Catherine Sermet, Steven Simoens, Robert Sauermann, Ad Schuurman, Ricardo Ramos, Vera Vlahovic-Palcevski, Corinne Zara, Brian Godman
Payers are a major stakeholder in any considerations and initiatives concerning adaptive licensing of new medicinal products, also referred to as Medicines Adaptive Pathways to patients (MAPPs). Firstly, the scope and necessity of MAPPs need further scrutiny, especially with regard to the definition of unmet need. Conditional approval pathways already exist for new medicines for seriously debilitating or life-threatening diseases and only a limited number of new medicines are innovative. Secondly, MAPPs will result in new medicines on the market with limited evidence about their effectiveness and safety...
2016: Frontiers in Pharmacology
Kamila L N Bandeira, Felipe Medeiros Simbras, Elaine Batista Machado, Diogenes de Almeida Campos, Gustavo R Oliveira, Alexander W A Kellner
Titanosaurian dinosaurs include some of the largest land-living animals that ever existed, and most were discovered in Cretaceous deposits of Argentina. Here we describe the first Brazilian gigantic titanosaur, Austroposeidon magnificus gen. et sp. nov., from the Late Cretaceous Presidente Prudente Formation (Bauru Group, Paraná Basin), São Paulo State, southeast Brazil. The size of this animal is estimated around 25 meters. It consists of a partial vertebral column composed by the last two cervical and the first dorsal vertebrae, all fairly complete and incomplete portions of at least one sacral and seven dorsal elements...
2016: PloS One
Aiswarya Viswanath, Julie Vanacker, Loïc Germain, Julian G Leprince, Anibal Diogenes, Kevin Shakesheff, Lisa J White, Anne des Rieux
Decellularised mammalian extracellular matrices (ECM) have been widely accepted as an ideal substrate for repair and remodelling of numerous tissues in clinical and pre-clinical studies. Recent studies have demonstrated the ability of ECM scaffolds derived from site-specific homologous tissues to direct cell differentiation. The present study investigated the suitability of hydrogels derived from different source tissues: bone, spinal cord and dentine, as suitable carriers to deliver human apical papilla derived mesenchymal stem cells (SCAP) for spinal cord regeneration...
September 16, 2016: Journal of Biomedical Materials Research. Part A
Jose Luis Rosário, Maria Suely Bezerra Diógenes, Rita Mattei, José Roberto Leite
Postural abnormalities can affect the emotions and vice-versa. The aim of the present study was to investigate the existence of a relationship between subjective anger and body posture in 28 women, aged between 20 and 39 years, with a normal body mass index (or underweight) and an absence of neurological, psychiatric or musculoskeletal disorders. The postural parameters photographed were the inclination of the shoulders, protrusion of the head, hyperextension of the knees and shoulder elevation. The degree of anger was rated by analogue scales representing current and usual anger...
July 2016: Journal of Bodywork and Movement Therapies
Bishoy Safwat Estefan, Kariem Mostafa El Batouty, Mohamed Mokhtar Nagy, Anibal Diogenes
INTRODUCTION: Treatment of immature permanent teeth with necrotic pulp and apical pathosis constitutes a challenge for endodontists. The present study was done to evaluate the effect of age and apical diameter on the regenerative potential of young permanent immature teeth with necrotic pulps. METHODS: Immature necrotic permanent maxillary incisors (n = 40) of patients 9-18 years old were divided into 2 groups according to the treatment protocol: group Y (younger age group), 9-13 years and group O (older age group), 14-18 years...
September 9, 2016: Journal of Endodontics
Nathalie Honorio-Felício, Marta S P Carepo, Tércio de F Paulo, Luiz Gonzaga de França Lopes, Eduardo H S Sousa, Izaura C N Diógenes, Paul V Bernhardt
Conformational changes associated to sensing mechanisms of heme-based protein sensors are a key molecular event that seems to modulate not only the protein activity but also the potential of the Fe(III/II) redox couple of the heme domain. In this work, midpoint potentials (Em) assigned to the Fe(III/II) redox couple of the heme domain of FixL from Rhizobium etli (ReFixL) in the unliganded and liganded states were determined by spectroelectrochemistry in the presence of inorganic mediators. In comparison to the unliganded ReFixL protein (+19mV), the binding to ligands that switch off the kinase activity induces a negative shift, i...
August 26, 2016: Journal of Inorganic Biochemistry
Obadah N Austah, Nikita B Ruparel, Michael A Henry, Roberto J Fajardo, James E Schmitz, Anibal Diogenes
INTRODUCTION: Nociceptive neurons play a critical role in the detection of stimuli evoking actual or potential tissue injury. In addition, they are involved in neurogenic inflammation by the peripheral release of neuropeptides such as calcitonin gene-related peptide (CGRP). The dental pulp and periradicular tissues are innervated by capsaicin-sensitive neurons known to release CGRP. However, the role of these capsaicin-sensitive neurons in the development of apical periodontitis is largely unknown...
October 2016: Journal of Endodontics
Changyong Yuan, Penglai Wang, Shaoyue Zhu, Ting Zou, Shuai Wang, Jianguang Xu, Boon Chin Heng, Anibal Diogenes, Chengfei Zhang
INTRODUCTION: This study aimed to investigate the roles of ephrinB2 in stabilizing vascularlike structures generated by stem cells from apical papilla (SCAPs) and human umbilical vein endothelial cells (HUVECs). METHODS: HUVECs were seeded alone or with SCAPs concurrently or 12 hours later. Angiogenesis and ephrinB2 phosphorylation were assayed at different time points. Additionally, ephrinB2 expression in SCAPs and HUVECs was silenced with small interfering RNA, and vascularlike structure formation within coculture was assessed; 1 × 10(5) HUVECs were seeded in transwell inserts, and 6 × 10(5) SCAPs were plated in lower wells with or without ephrinB2-Fc...
September 2016: Journal of Endodontics
Marcos A S de Oliveira, Diógenes S Moura, Adriana Fontes, Renato E de Araujo
No abstract text is available yet for this article.
July 1, 2016: Journal of Biomedical Optics
Sheila Lima Diógenes Santos, Luciano Brito Santos, Viriato Campelo, Ana Roberta Vilarouca da Silva
Purpose Identify factors associated with infant mortality by a hierarchical model based on socioeconomic, health care, obstetric and biological determinants in a northeastern Brazilian capital. Methods Observational, retrospective cohort study based on secondary data of births and deaths of infants of mothers living in the city of Teresina. Results Based on the distal level of determination of infant mortality, the characteristics that remained statistically significant were maternal age, maternal education and maternal occupation (p < 0...
July 18, 2016: Revista Brasileira de Ginecologia e Obstetrícia
Francisco Andrade Neto, Manuel Joaquim Diógenes Teixeira, Leonardo Heráclio do Carmo Araújo, Carlos Eduardo Barbosa Ponte
The knee is a common site for bone tumors, whether clinically painful or not. Conventional radiology has been established as the first line of investigation in patients with knee pain and can reveal lesions that often generate questions not only for the generalist physician but also for the radiologist or general orthopedist. History, image examination, and histopathological analysis compose the essential tripod of the diagnosis of bone tumors, and conventional radiology is an essential diagnostic tool in patients with knee pain...
May 2016: Radiologia Brasileira
Ivan Marin
The holotype of Clibanarius sachalinicus Kobjakova, 1955 and Clibanarius hirsutimanus Kobjakova, 1971 in the collection of Zoological Institute of Russian Academy of Sciences (Saint-Petersburg, Russia) are examined. As a result, Clibanarius hirsutimanus Kobjakova, 1971 is found to be a species of Areopaguristes nigroapiculus (Komai, 2009) while Clibanarius sachalinicus Kobjakova, 1955 is a species of Clibanarius virescens (Krauss, 1843). Remarks on a single record of Diogenes penicillatus Stimpson, 1858 from Shamora Bay (Usury Bay) of the Sea of Japan as well as current faunal list of hermit crabs from the Russian coasts of the Sea of Japan are also presented in the paper...
2016: Zootaxa
Diógenes Aparício Garcia Cortez, Érica Simionato Machado, Sonia Cristina Soares Dias Vermelho, Jorge Juarez Vieira Teixeira, Lucia Elaine Ranieri Cortez
The scope of this research was to establish the level of awareness of beauticians regarding the importance of the application of sunscreen and to identify whether their patients had been properly instructed by these professionals. It involved a descriptive and exploratory study with interviews applying qualitative methodology among 30 beauticians. Data were gathered using the semi-structured interview technique in Maringá, in the southern state of Paraná. The data were analyzed using Atlas.ti software after applying quantitative analysis and response classification...
June 2016: Ciência & Saúde Coletiva
Valnês S Rodrigues-Junior, Anne D Villela, Raoni S B Gonçalves, Bruno Lopes Abbadi, Rogério Valim Trindade, Alexandre López-Gavín, Griselda Tudó, Julian González-Martín, Luiz Augusto Basso, Marcus V N de Souza, Maria Martha Campos, Diógenes Santiago Santos
Repurposing of drugs to treat tuberculosis (TB) has been considered an alternative to overcome the global TB epidemic, especially to combat drug-resistant forms of the disease. Mefloquine has been reported as a potent drug to kill drug-resistant strains of Mycobacterium tuberculosis. In addition, mefloquine-derived molecules have been synthesised and their effectiveness against mycobacteria has been assessed. In this work, we demonstrate for the first time the activities of mefloquine and its oxazolidine derivative compound 1E in a murine model of TB infection following administration of both drugs by the oral route...
August 2016: International Journal of Antimicrobial Agents
Adolfo I B Romo, Dieric S Abreu, Tércio de F Paulo, Marta S P Carepo, Eduardo H S Sousa, Luis Lemus, Carolina Aliaga, Alzir A Batista, Otaciro R Nascimento, Héctor D Abruña, Izaura C N Diógenes
Coordination compounds of copper have been invoked as major actors in processes involving the reduction of molecular oxygen, mostly with the generation of radical species the assignment for which has, so far, not been fully addressed. In the present work, we have carried out studies in solution and on surfaces to gain insights into the nature of the radical oxygen species (ROS) generated by a copper(II) coordination compound containing a thioether clip-phen derivative, 1,3-bis(1,10-phenanthrolin-2-yloxy)-N-(4-(methylthio)benzylidene)propan-2-amine (2CP-Bz-SMe), enabling its adsorption/immobilization to gold surfaces...
July 11, 2016: Chemistry: a European Journal
Isabel Clarisse Albuquerque Gonzaga, Sheila Lima Diogenes Santos, Ana Roberta Vilarouca da Silva, Viriato Campelo
The main determinants of the risk of mortality in the neonatal period are low birth weight and premature birth. The study sought to analyze the adequacy of prenatal care and risk factors associated with premature birth and low birth weight in a northeastern Brazilian capital. This is a case-control study. A model for adequacy of prenatal conditions composed of four indicators was created. Descriptive statistics for univariate analysis were used; as well as Wald linear trend tests, Student's t and chi-square test for bivariate analysis and multiple logistic regression for multivariate analysis with p <0...
June 2016: Ciência & Saúde Coletiva
Rogério V Trindade, Antônio F M Pinto, Diógenes S Santos, Cristiano V Bizarro
In recent years, phenotypic screening has assumed a leading role in drug discovery efforts. However, development of new drugs from bioactive compounds obtained in screening campaigns requires identification of the cellular targets responsible for their biological activities. A new energetics-based method for target identification is presented: pulse proteolysis and precipitation for target identification (PePTID). In this method, proteins incubated with or without a ligand and submitted to a brief proteolytic pulse are directly analyzed and compared using a label-free semiquantitative mass spectrometry strategy, dispensing the SDS-PAGE readout and greatly improving the throughput...
July 1, 2016: Journal of Proteome Research
Sondes Melliti Ben Garali, Inès Sahraoui, Pablo de la Iglesia, Mohamed Chalghaf, Jorge Diogène, Jamel Ksouri, Asma Sakka Hlaili
The effects of inorganic and organic nitrogen supply on the growth and domoic acid (DA) production of Pseudo-nitzschia cf. seriata and Pseudo-nitzschia calliantha from Bizerte Lagoon (SW Mediterranean Sea) were studied during field and laboratory experiments. Nitrogen enrichments (40 µM NO3 (-); 10 µM NH4 (+); 20 µM CH4N2O) and a control, with no added N, were carried out in separate carboys with seawater collected from Bizerte Lagoon. In the field experiments, all N-enrichments resulted in significant increases in chlorophyll a concentration, and maintained exponential growth until the end of the experiment...
August 2016: Ecotoxicology
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