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https://www.readbyqxmd.com/read/29707740/biotechnology-applied-to-fish-reproduction-tools-for-conservation
#1
Diógenes Henrique de Siqueira-Silva, Taiju Saito, Amanda Pereira Dos Santos-Silva, Raphael da Silva Costa, Martin Psenicka, George Shigueki Yasui
This review discusses the new biotechnological tools that are arising and promising for conservation and enhancement of fish production, mainly regarding the endangered and the most economically important species. Two main techniques, in particular, are available to avoid extinction of endangered fish species and to improve the production of commercial species. Germ cell transplantation technology includes a number of approaches that have been studied, such as the transplantation of embryo-to-embryo blastomere, embryo-to-embryo differentiated PGC, larvae to larvae and embryo differentiated PGC, transplantation of spermatogonia from adult to larvae or between adults, and oogonia transplantation...
April 29, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29695962/inhibition-of-nmda-receptors-prevents-the-loss-of-bdnf-function-induced-by-amyloid-%C3%AE
#2
Sara R Tanqueiro, Rita M Ramalho, Tiago M Rodrigues, Luísa V Lopes, Ana M Sebastião, Maria J Diógenes
Brain-derived neurotrophic factor (BDNF) plays important functions in cell survival and differentiation, neuronal outgrowth and plasticity. In Alzheimer's disease (AD), BDNF signaling is known to be impaired, partially because amyloid β (Aβ) induces truncation of BDNF main receptor, TrkB-full length (TrkB-FL). We have previously shown that such truncation is mediated by calpains, results in the formation of an intracellular domain (ICD) fragment and causes BDNF loss of function. Since calpains are Ca2+ -dependent proteases, we hypothesized that excessive intracellular Ca2+ build-up could be due to dysfunctional N-methyl-d-aspartate receptors (NMDARs) activation...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29689988/a-new-species-of-the-hermit-crab-genus-diogenes-crustacea-decapoda-diogenidae-from-the-coast-of-kwazulu-natal-south-africa
#3
Jannes Landschoff, Dwi Listyo Rahayu
A new diogenid hermit crab with bright-white chelae, Diogenes albimanus n. sp., is described and illustrated, based on a single ovigerous female collected from KwaZulu-Natal, South Africa. The new species is compared to morphologically similar species, in particular to D. holthuisi and D. dorotheae, from which it is distinguishable by the shape and armature of the telson; and also to the superficially-similar South African D. extricatus, from which it differs by having the unarmed dorsal margins of the propodi and meri of the pereopods, as opposed to being armed with rows of strong spines...
February 13, 2018: Zootaxa
https://www.readbyqxmd.com/read/29689295/behavioral-and-biochemical-effects-of-ethanol-withdrawal-in-zebrafish
#4
Suianny Nayara da Silva Chaves, Gabriel Felício Rocha, Bruna Patrícia Dutra Costa, Witallo Etevaldo Araújo de Oliveira, Monica Gomes Lima, Diógenes Henrique de Siqueira Silva, Caio Maximino
Chronic alcohol use induces adaptations and toxicity that can induce symptoms of anxiety, autonomic hyperarousal, and epileptic seizures when alcohol is removed (withdrawal syndrome). Zebrafish has recently gained wide attention as a behavioral model to study the neurobehavioral effects of acute and chronic alcohol use, including withdrawal. The literature, however, is very contradictory on findings regarding withdrawal effects, with some studies reporting increased anxiety, while others report no effect. A meta-analytic approach was taken to find the sources of this heterogeneity, and ethanol concentration during exposure and exposure duration were found to be the main sources of variation...
April 21, 2018: Pharmacology, Biochemistry, and Behavior
https://www.readbyqxmd.com/read/29668793/new-crocodyliform-specimens-from-rec%C3%A3-ncavo-tucano-basin-early-cretaceous-of-bahia-brazil
#5
Rafael G DE Souza, Diogenes A Campos
In 1940, L.I. Price and A. Oliveira recovered four crocodyliform specimens from the Early Cretaceous Bahia Supergroup (Recôncavo-Tucano Basin). In the present work, we describe four different fossil specimens: an osteoderm, a fibula, a tibia, and some autopodial bones. No further identification besides Mesoeucrocodylia was made due to their fragmentary nature and the reduced number of recognized synapomorphies for more inclusive clades. With exception of the fibula, all other specimens have at least one particular feature, which with new specimens could represent new species...
April 16, 2018: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29652533/facial-and-nasolabial-aesthetics-of-complete-uclp-submitted-to-2-stage-palate-repair-with-vomer-flap
#6
Terumi Okada Ozawa, Luciana Lais Savero Reis, Renata Mayumi Kato, Diógenes Laercio Rocha, Renata Sathler, Daniela Gamba Garib
OBJECTIVE: To evaluate the aesthetics of nasolabial appearance and facial profile of children with unilateral cleft lip and palate (UCLP) submitted to 2-stage palate repair with vomerine flap. DESIGN: Retrospective. SETTING: Single center. PATIENTS: Forty patients with UCLP, mean age of 7.81 years of both sexes, rehabilitated at a single center by 1 plastic surgeon. INTERVENTIONS: Lip and anterior palate repair with nasal alar repositioning was performed at 3 to 6 months of age by Millard technique and vomer flap, respectively...
January 1, 2018: Cleft Palate-craniofacial Journal
https://www.readbyqxmd.com/read/29619113/analysis-of-circulating-angiopoietin-like-protein-3-and-genetic-variants-in-lipid-metabolism-and-liver-health-the-diogenes-study
#7
Anne Lundby Hess, Jérôme Carayol, Trine Blædel, Jörg Hager, Alessandro Di Cara, Arne Astrup, Wim H M Saris, Lesli Hingstrup Larsen, Armand Valsesia
Background: Angiopoietin-like protein 3 (ANGPTL3), a liver-derived protein, plays an important role in the lipid and lipoprotein metabolism. Using data available from the DiOGenes study, we assessed the link with clinical improvements (weight, plasma lipid, and insulin levels) and changes in liver markers, alanine aminotransferase, aspartate aminotransferase (AST), adiponectin, fetuin A and B, and cytokeratin 18 (CK-18), upon low-calorie diet (LCD) intervention. We also examined the role of genetic variation in determining the level of circulating ANGPTL3 and the relation between the identified genetic markers and markers of hepatic steatosis...
2018: Genes & Nutrition
https://www.readbyqxmd.com/read/29608932/assessing-the-role-of-deod-gene-in-mycobacterium-tuberculosis-in-vitro-growth-and-macrophage-infection
#8
Pedro Ferrari Dalberto, Valnês Rodrigues-Junior, Virginia Carla Almeida Falcão, Antônio Frederico Michel Pinto, Bruno Lopes Abbadi, Cristiano Valim Bizarro, Luiz Augusto Basso, Anne Drumond Villela, Diógenes Santiago Santos
Purine nucleoside phosphorylase from Mycobacterium tuberculosis (MtPNP), encoded by deoD gene (Rv3307), is an enzyme from the purine salvage pathway, which has been widely studied as a molecular target for the development of inhibitors with potential antimycobacterial activity. However, the role of MtPNP in tuberculosis pathogenesis and dormancy is still unknown. The present work aims to construct a deoD knockout strain from M. tuberculosis, to evaluate the role of MtPNP in the growth of M. tuberculosis under oxygenated condition and in a dormancy model, and to assess whether deoD gene is important for M...
March 30, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29589574/synchronizing-developmental-stages-in-neotropical-catfishes-for-application-in-germ-cell-transplantation
#9
Dilberto Ribeiro Arashiro, George Shigueki Yasui, Leonardo Luiz Calado, Nivaldo Ferreira do Nascimento, Matheus Pereira Dos Santos, Silvio Carlos Alves do Santos, Nycolas Levy-Pereira, Paulo Sérgio Monzani, Diógenes Henrique Siqueira-Silva, José Augusto Senhorini
SummaryThe aim of this study was to describe the effect of temperature on the fertilization, early developmental stages, and survival rate of two Neotropical catfishes Pimelodus maculatus and Pseudopimelodus mangurus. After fertilization, the eggs were incubated at 22°C, 26°C, and 30°C, which resulted in fertilization rates of 96.95 ± 1.79%, 98.74 ± 0.76%, and 98.44 ± 0.19% for P. maculatus and 96.10 ± 1.58%, 98.00 ± 0.63%, and 94.60 ± 2.09% for P. mangurus, respectively. For P. maculatus, hatching occurred after 22 h 30 min post-fertilization at 22°C, 16 h 30 min at 26°C, and 11 h 20 min at 30°C, and the hatching rates were 43...
March 28, 2018: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29588162/aminochrome-decreases-ngf-gdnf-and-induces-neuroinflammation-in-organotypic-midbrain-slice-cultures
#10
Fillipe M de Araújo, Rafael S Ferreira, Cleide S Souza, Cleonice Creusa Dos Santos, Tácio L R S Rodrigues, Juliana Helena C E Silva, Juciano Gasparotto, Daniel Pens Gelain, Ramon S El-Bachá, Maria de Fátima D Costa, José Claudio M Fonseca, Juan Segura-Aguilar, Silvia L Costa, Victor Diogenes A Silva
Recent evidence shows that aminochrome induces glial activation related to neuroinflammation. This dopamine derived molecule induces formation and stabilization of alpha-synuclein oligomers, mitochondria dysfunction, oxidative stress, dysfunction of proteasomal and lysosomal systems, endoplasmic reticulum stress and disruption of the microtubule network, but until now there has been no evidence of effects on production of cytokines and neurotrophic factors, that are mechanisms involved in neuronal loss in Parkinson's disease (PD)...
March 24, 2018: Neurotoxicology
https://www.readbyqxmd.com/read/29573994/vectors-and-reservoir-hosts-of-covert-mortality-nodavirus-cmnv-in-shrimp-ponds
#11
Shuang Liu, Xiuhua Wang, TingTing Xu, Xiaoping Li, Lichun Du, Qingli Zhang
Covert mortality nodavirus (CMNV) is the pathogen that has been identified as the cause of viral covert mortality disease (VCMD) in marine and brackish water shrimp. Recent outbreaks of this disease have resulted, and continue to result, in substantial production and economic losses to shrimp aquaculture producers in China and elsewhere. To explore potential vectors and reservoir hosts of CMNV, we collected fifteen species of invertebrates from shrimp ponds affected by VCMD. Samples were tested through the use of: reverse transcription loop-mediated isothermal amplification (RT-LAMP), reverse transcription nested PCR (RT-nPCR) followed by gene sequencing, histopathology, and in situ RNA hybridization (ISH)...
March 21, 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29569030/author-correction-stem-cells-from-human-apical-papilla-decrease-neuro%C3%A2-inflammation-and-stimulate-oligodendrocyte-progenitor-differentiation-via-activin%C3%A2-a-secretion
#12
Pauline De Berdt, Pauline Bottemanne, John Bianco, Mireille Alhouayek, Anibal Diogenes, Amy Lloyd, Jose Gerardo-Nava, Gary A Brook, Véronique Miron, Giulio G Muccioli, Anne des Rieux
In the original publication, sixth author's surname was incorrectly published as "Llyod" instead of "Lloyd". The correct name should read as "Amy Lloyd".
March 22, 2018: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29559160/a-multivariate-approach-to-the-diagnosis-of-gastrointestinal-infection-in-ewes
#13
Josiel Borges Ferreira, Renato Diógenes Macedo Paiva, Ana Carla Diógenes Suassuna Bezerra, José Ernandes Rufino de Sousa, Débora Andréa Evangelista Façanha
Diagnosing the effects of helminth infections is an efficient way to manage the problem in ruminant production systems. Selection of animals in a flock that are infected with Haemonchus has traditionally involved differential packed cell volume measurements (PCV), and in recent years, the FAMACHA© system has been used. However, few small analytical changes have been made. The objective of this study was to examine the influence of different diagnoses linked to packed cell volume (PCV) and FAMACHA© system scores in native ewes, using a multivariate approach...
March 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29559112/effect-of-the-bradykinin-1-receptor-antagonist-ssr240612-after-oral-administration-in-mycobacterium-tuberculosis-infected-mice
#14
Valnês S Rodrigues-Junior, Priscilla B Pail, Anne D Villela, Virgínia C A Falcão, Adílio S Dadda, Bruno L Abbadi, João B Pesquero, Diógenes S Santos, Luiz A Basso, Maria M Campos
The role, if any, played by the kinin system in tuberculosis infection models, either in vivo or in vitro, was investigated. The effects of Mycobacterium tuberculosis infection on C57BL/6 wild type, B1 R-/-, B2 R-/- and double B1 R/B2 R knockout mice were evaluated. Immunohistochemistry analysis was carried out to assess B1 R and B2 R expression in spleens and lungs of M. tuberculosis-infected mice. In addition, in vitro experiments with M. tuberculosis-infected macrophages were performed. The in vivo effects of HOE-140 and SSR240612 on the mice model of infection were also evaluated...
March 2018: Tuberculosis
https://www.readbyqxmd.com/read/29471068/weight-loss-decreases-self-reported-appetite-and-alters-food-preferences-in-overweight-and-obese-adults-observational-data-from-the-diogenes-study
#15
Charlotte Andriessen, Pia Christensen, Lone Vestergaard Nielsen, Christian Ritz, Arne Astrup, Thomas Meinert Larsen, J Alfredo Martinez, Wim H M Saris, Marleen A van Baak, Angeliki Papadaki, Marie Kunesova, Susan Jebb, John Blundell, Clare Lawton, Anne Raben
People with obesity often struggle to maintain their weight loss after a weight loss period. Furthermore, the effect of weight loss on appetite and food preferences remains unclear. Hence this study investigated the effect of weight loss on subjective appetite and food preferences in healthy, overweight and obese volunteers. A subgroup of adult participants (n = 123) from the Diet Obesity and Genes (DiOGenes) study (subgroup A) was recruited from across six European countries. Participants lost ≥8% of initial body weight during an 8-week low calorie diet (LCD)...
June 1, 2018: Appetite
https://www.readbyqxmd.com/read/29465419/assessment-of-pcxmc-for-patients-with-different-body-size-in-chest-and-abdominal-x-ray-examinations-a-monte-carlo-simulation-study
#16
David Borrego, Erin M Lowe, Cari M Kitahara, Choonsik Lee
A PC Program for x ray Monte Carlo (PCXMC) has been used to calculate organ doses in patient dosimetry and for the exposure assessment in epidemiological studies of radiogenic health related risks. This study compared the dosimetry from using the built-in stylized phantoms in the PCXMC to that of a newer hybrid phantom library with improved anatomical realism. We simulated chest and abdominal x ray projections for 146 unique body size computational phantoms, 77 males and 69 females, with different combinations of height (125-180 cm) and weight (20-140 kg) using the built-in stylized phantoms in the PCXMC version 2...
March 21, 2018: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29458351/cemitool-a-bioconductor-package-for-performing-comprehensive-modular-co-expression-analyses
#17
Pedro S T Russo, Gustavo R Ferreira, Lucas E Cardozo, Matheus C Bürger, Raul Arias-Carrasco, Sandra R Maruyama, Thiago D C Hirata, Diógenes S Lima, Fernando M Passos, Kiyoshi F Fukutani, Melissa Lever, João S Silva, Vinicius Maracaja-Coutinho, Helder I Nakaya
BACKGROUND: The analysis of modular gene co-expression networks is a well-established method commonly used for discovering the systems-level functionality of genes. In addition, these studies provide a basis for the discovery of clinically relevant molecular pathways underlying different diseases and conditions. RESULTS: In this paper, we present a fast and easy-to-use Bioconductor package named CEMiTool that unifies the discovery and the analysis of co-expression modules...
February 20, 2018: BMC Bioinformatics
https://www.readbyqxmd.com/read/29426646/effect-of-3-bioceramic-materials-on-stem-cells-of-the-apical-papilla-proliferation-and-differentiation-using-a-dentin-disk-model
#18
Amber A Miller, Koyo Takimoto, James Wealleans, Anibal Diogenes
INTRODUCTION: There is a complex interaction between biomaterials placed as a coronal barrier with stem cells and dentin in regenerative procedures. In this study, the effect of Biodentine (BD; Septodont, Saint-Maurdes-Fossés, France), Endosequence BC Root Repair Material-Putty (ES; Brasseler, Savannah, GA), Endosequence BC Root Repair Material-Putty Fast set (ES-fast, Brasseler), and ProRoot (Dentsply Tulsa Dental Specialties, Johnson City, TN) mineral trioxide aggregate (MTA) on the viability and differentiation of stem cells of the apical papilla (SCAP) was evaluated using an ex vivo dentin disk model...
April 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29425760/agathisflavone-a-flavonoid-derived-from-poincianella-pyramidalis-tul-enhances-neuronal-population-and-protects-against-glutamate-excitotoxicity
#19
Cleide Dos Santos Souza, Maria Socorro Grangeiro, Erica Patricia Lima Pereira, Cleonice Creusa Dos Santos, Alessandra Bispo da Silva, Geraldo Pedral Sampaio, Daiana Dias Ribeiro Figueiredo, Jorge Mauricio David, Juceni Pereira David, Victor Diogenes Amaral da Silva, Arthur Morgan Butt, Silvia Lima Costa
Flavonoids are bioactive compounds that are known to be neuroprotective against glutamate-mediated excitotoxicity, one of the major causes of neurodegeneration. The mechanisms underlying these effects are unresolved, but recent evidence indicates flavonoids may modulate estrogen signaling, which can delay the onset and ameliorate the severity of neurodegenerative disorders. Furthermore, the roles played by glial cells in the neuroprotective effects of flavonoids are poorly understood. The aim of this study was to investigate the effects of the flavonoid agathisflavone (FAB) in primary neuron-glial co-cultures from postnatal rat cerebral cortex...
March 2018: Neurotoxicology
https://www.readbyqxmd.com/read/29417177/stem-cells-from-human-apical-papilla-decrease-neuro-inflammation-and-stimulate-oligodendrocyte-progenitor-differentiation-via-activin-a-secretion
#20
Pauline De Berdt, Pauline Bottemanne, John Bianco, Mireille Alhouayek, Anibal Diogenes, Amy Llyod, Jose Gerardo-Nava, Gary A Brook, Véronique Miron, Giulio G Muccioli, Anne des Rieux
Secondary damage following spinal cord injury leads to non-reversible lesions and hampering of the reparative process. The local production of pro-inflammatory cytokines such as TNF-α can exacerbate these events. Oligodendrocyte death also occurs, followed by progressive demyelination leading to significant tissue degeneration. Dental stem cells from human apical papilla (SCAP) can be easily obtained at the removal of an adult immature tooth. This offers a minimally invasive approach to re-use this tissue as a source of stem cells, as compared to biopsying neural tissue from a patient with a spinal cord injury...
February 7, 2018: Cellular and Molecular Life Sciences: CMLS
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