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https://www.readbyqxmd.com/read/27900567/clinical-potential-of-human-induced-pluripotent-stem-cells-perspectives-of-induced-pluripotent-stem-cells
#1
REVIEW
Dharmendra Kumar, Taruna Anand, Wilfried A Kues
The recent establishment of induced pluripotent stem (iPS) cells promises the development of autologous cell therapies for degenerative diseases, without the ethical concerns associated with human embryonic stem (ES) cells. Initially, iPS cells were generated by retroviral transduction of somatic cells with core reprogramming genes. To avoid potential genotoxic effects associated with retroviral transfection, more recently, alternative non-viral gene transfer approaches were developed. Before a potential clinical application of iPS cell-derived therapies can be planned, it must be ensured that the reprogramming to pluripotency is not associated with genome mutagenesis or epigenetic aberrations...
November 29, 2016: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/27856246/reprogramming-of-retinoblastoma-cancer-cells-into-cancer-stem-cells
#2
Fengming Yue, Kanji Hirashima, Daihachiro Tomotsune, Sakiko Takizawa-Shirasawa, Tadayuki Yokoyama, Katsunori Sasaki
Retinoblastoma is the most common intraocular malignancy in pediatric patients. It develops rapidly in the retina and can be fatal if not treated promptly. It has been proposed that a small population of cancer cells, termed cancer stem cells (CSCs), initiate tumorigenesis from immature tissue stem cells or progenitor cells. Reprogramming technology, which can convert mature cells into pluripotent stem cells (iPS), provides the possibility of transducing malignant cancer cells back to CSCs, a type of early stage of cancer...
November 14, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27826858/analysis-of-protein-protein-interactions-between-men-components
#3
Rami Rahal
The isolation of protein complexes is a powerful method to elucidate how signaling networks are regulated. Co-immunoprecipitation (co-IP) is a widely used procedure to detect and characterize the physical association of pathway components that has frequently been used to reveal mechanisms of pathway regulation. This chapter outlines the roles of protein-protein interactions in signal transduction and the regulatory functions such assemblies impart to cells. The principles behind co-IPs are discussed and best practices for conducting and interpreting co-IPs experiments are outlined...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27788138/towards-a-safer-more-randomized-lentiviral-vector-integration-profile-exploring-artificial-ledgf-chimeras
#4
Lenard S Vranckx, Jonas Demeulemeester, Zeger Debyser, Rik Gijsbers
The capacity to integrate transgenes into the host cell genome makes retroviral vectors an interesting tool for gene therapy. Although stable insertion resulted in successful correction of several monogenic disorders, it also accounts for insertional mutagenesis, a major setback in otherwise successful clinical gene therapy trials due to leukemia development in a subset of treated patients. Despite improvements in vector design, their use is still not risk-free. Lentiviral vector (LV) integration is directed into active transcription units by LEDGF/p75, a host-cell protein co-opted by the viral integrase...
2016: PloS One
https://www.readbyqxmd.com/read/27572712/induced-pluripotent-stem-ips-cells-in-dentistry-a-review
#5
REVIEW
Neeraj Malhotra
iPS cells are derived from somatic cells via transduction and expression of selective transcription factors. Both viral-integrating (like retroviral) and non-integrating (like, mRNA or protein-based) techniques are available for the production of iPS cells. In the field of dentistry, iPS cells have been derived from stem cells of apical papilla, dental pulp stem cells, and stem cells from exfoliated deciduous teeth, gingival and periodontal ligament fibroblasts, and buccal mucosa fibroblasts. iPS cells have the potential to differentiate into all derivatives of the 3 primary germ layers i...
November 30, 2016: International Journal of Stem Cells
https://www.readbyqxmd.com/read/27456042/potential-role-of-d-myo-inositol-3-phosphate-synthase-and-14-3-3-genes-in-the-crosstalk-between-zea-mays-and-rhizophagus-intraradices-under-drought-stress
#6
Tao Li, Yuqing Sun, Yuan Ruan, Lijiiao Xu, Yajun Hu, Zhipeng Hao, Xin Zhang, Hong Li, Youshan Wang, Liguo Yang, Baodong Chen
Arbuscular mycorrhizal (AM) symbiosis is known to stimulate plant drought tolerance. However, the mechanisms underlying the synergistic responses of the symbiotic partners to drought stress are largely unknown. A split-root experiment was designed to investigate the molecular interactions between a host plant and an AM fungus (AMF) under drought stress. In the two-compartment cultivation system, an entire or only a half root system of a maize plant was inoculated with an AMF, Rhizophagus intraradices, in the presence of localized or systemic drought treatment...
November 2016: Mycorrhiza
https://www.readbyqxmd.com/read/27345971/peripheral-blood-mononuclear-cell-converted-induced-pluripotent-stem-cells-ipscs-from-an-early-onset-alzheimer-s-patient
#7
Han-Kyu Lee, Peter Morin, Weiming Xia
Improvement in transduction efficiency makes it possible to convert blood cells into induced pluripotent stem cells (iPSC). In this study, we generated an iPSC line from peripheral blood mononuclear cells (PBMC) donated by a patient who exhibited memory deficit at age 59; outcome of positron emission tomography scan is consistent with a diagnosis of Alzheimer's disease. Integration-free CytoTune-iPS Sendai Reprogramming factors which include Sendai virus particles of the four Yamanaka factors Oct4, Sox2, Klf4, and c-Myc were introduced to PBMC to convert them to iPSCs without retention of virus...
March 2016: Stem Cell Research
https://www.readbyqxmd.com/read/27193760/a-rapid-and-efficient-polyethylenimine-based-transfection-method-to-prepare-lentiviral-or-retroviral-vectors-useful-for-making-ips-cells-and-transduction-of-primary-cells
#8
Shaozhe Yang, Haijun Shi, Xinran Chu, Xiaoling Zhou, Pingnan Sun
OBJECTIVES: To improve the efficiency, reproducibility and consistency of the PEI-based transfection method that is often used in preparation of recombinant lentiviral or retroviral vectors. RESULTS: The contributions to transfection efficiency of multi-factors including concentration of PEI or DNA, dilution buffer for PEI/DNA, manner to prepare PEI/DNA complexes, influence of serum, incubation time for PEI/DNA complexes, and transfection time were studied. Gentle mixing during the preparation of PEI/DNA transfection complexes is critical for a high transfection efficiency...
September 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/26987925/generation-of-ipsc-lines-from-a-nijmegen-breakage-syndrome-patient
#9
Barbara Mlody, James Adjaye
Human dermal fibroblasts from a Nijmegen Breakage Syndrome (NBS) patient bearing the 657del5 mutation within the DNA repair gene NIBRIN were used to generate two iPSC-lines (vNBS8-iPS-c1, vNBS8-iPS-c2) by retroviral transduction of OCT4, SOX2, c-MYC and KLF4. Pluripotency was confirmed both in vivo and in vitro.
November 2015: Stem Cell Research
https://www.readbyqxmd.com/read/26911482/analysis-of-weighted-co-regulatory-networks-in-maize-provides-insights-into-new-genes-and-regulatory-mechanisms-related-to-inositol-phosphate-metabolism
#10
Shaojun Zhang, Wenzhu Yang, Qianqian Zhao, Xiaojin Zhou, Ling Jiang, Shuai Ma, Xiaoqing Liu, Ye Li, Chunyi Zhang, Yunliu Fan, Rumei Chen
BACKGROUND: D-myo-inositol phosphates (IPs) are a series of phosphate esters. Myo-inositol hexakisphosphate (phytic acid, IP6) is the most abundant IP and has negative effects on animal and human nutrition. IPs play important roles in plant development, stress responses, and signal transduction. However, the metabolic pathways and possible regulatory mechanisms of IPs in maize are unclear. In this study, the B73 (high in phytic acid) and Qi319 (low in phytic acid) lines were selected for RNA-Seq analysis from 427 inbred lines based on a screening of IP levels...
2016: BMC Genomics
https://www.readbyqxmd.com/read/26849918/emerging-landscape-of-cell-penetrating-peptide-in-reprogramming-and-gene-editing
#11
REVIEW
Huiting Liu, Fanhui Zeng, Ming Zhang, Fajun Huang, Jiajun Wang, Jingjing Guo, Changbai Liu, Hu Wang
The plasma membrane remains a major barrier for intracellular drug delivery, to overcome this issue, a variety of approaches have been developed and used to deliver therapeutic cargos. Among these approaches, cell penetrating peptide (CPP) is promising and affords widely used vector for efficient intracellular delivery of cargos. Moreover, the latter findings including iPS reprogramming and direct transdifferentiation as well as gene editing have gradually become hot research topic; because their application in tissue engineering and disease modeling have great potential to advance innovation in precision medicine...
March 28, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/26679606/hepatic-maturation-of-human-ips-cell-derived-hepatocyte-like-cells-by-atf5-c-ebp%C3%AE-and-prox1-transduction
#12
Daiki Nakamori, Kazuo Takayama, Yasuhito Nagamoto, Seiji Mitani, Fuminori Sakurai, Masashi Tachibana, Hiroyuki Mizuguchi
Hepatocyte-like cells differentiated from human iPS cells (human iPS-HLCs) are expected to be utilized in drug development and research. However, recent hepatic characterization of human iPS-HLCs showed that these cells resemble fetal hepatocytes rather than adult hepatocytes. Therefore, in this study, we aimed to develop a method to enhance the hepatic function of human iPS-HLCs. Because the gene expression levels of the hepatic transcription factors (activating transcription factor 5 (ATF5), CCAAT/enhancer-binding protein alpha (c/EBPα), and prospero homeobox protein 1 (PROX1)) in adult liver were significantly higher than those in human iPS-HLCs and fetal liver, we expected that the hepatic functions of human iPS-HLCs could be enhanced by adenovirus (Ad) vector-mediated ATF5, c/EBPα, and PROX1 transduction...
January 15, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/26561805/cell-penetrating-peptide-as-a-means-of-directing-the-differentiation-of-induced-pluripotent-stem-cells
#13
REVIEW
Taku Kaitsuka, Kazuhito Tomizawa
Protein transduction using cell-penetrating peptides (CPPs) is useful for the delivery of large protein molecules, including some transcription factors. This method is safer than gene transfection methods with a viral vector because there is no risk of genomic integration of the exogenous DNA. Recently, this method was reported as a means for the induction of induced pluripotent stem (iPS) cells, directing the differentiation into specific cell types and supporting gene editing/correction. Furthermore, we developed a direct differentiation method to obtain a pancreatic lineage from mouse and human pluripotent stem cells via the protein transduction of three transcription factors, Pdx1, NeuroD, and MafA...
2015: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/26450367/the-effect-of-fetal-bovine-serum-fbs-on-efficacy-of-cellular-reprogramming-for-induced-pluripotent-stem-cell-ipsc-generation
#14
Daekee Kwon, Jin-Su Kim, Byung-Hyun Cha, Kwang-Sook Park, Inbo Han, Kyung-Soon Park, Hojae Bae, Myung-Kwan Han, Kwang-Soo Kim, Soo-Hong Lee
Induced pluripotent stem cells (iPSCs) are pivotal to the advancement of regenerative medicine. However, the low efficacy of iPSC generation and insufficient knowledge about the reprogramming mechanisms involved in somatic cell/adult stem cell reversion to a pluripotent phenotype remain critical hurdles to the therapeutic application of iPSCs. The present study investigated whether the concentration of fetal bovine serum (FBS), a widely employed cell culture additive, can influence the cellular reprogramming efficacy (RE) of human adipose-derived stem cells (hADSCs) to generate iPSCs...
2016: Cell Transplantation
https://www.readbyqxmd.com/read/26437794/proteolysis-of-mda5-and-ips-1-is-not-required-for-inhibition-of-the-type-i-ifn-response-by-poliovirus
#15
Swathi Kotla, Kurt E Gustin
BACKGROUND: The type I interferon (IFN) response is a critical component of the innate immune response to infection by RNA viruses and is initiated via recognition of viral nucleic acids by RIG-like receptors (RLR). Engagement of these receptors in the cytoplasm initiates a signal transduction pathway leading to activation of the transcription factors NF-κB, ATF-2 and IRF-3 that coordinately upregulate transcription of type I IFN genes, such as that encoding IFN-β. In this study the impact of poliovirus infection on the type I interferon response has been examined...
October 6, 2015: Virology Journal
https://www.readbyqxmd.com/read/26301418/transposon-based-reprogramming-to-induced-pluripotency
#16
REVIEW
Dharmendra Kumar, Thirumala R Talluri, Taruna Anand, Wilfried A Kues
Induced pluripotent stem (iPS) cells represent a recent innovation in the field of stem cells. Commonly, iPS cells are generated by viral transduction of core reprogramming genes, such as Oct4, Sox2, Klf4, c-Myc, Nanog and Lin28. However, integrating viruses, like retro- and lentiviral vectors, may cause insertional mutagenesis and may increase the risk of tumor formation. Therefore, alternative methods which avoid these safety concerns are intensively investigated. Here, we review the current status of transposon-based methods to induce pluripotency...
December 2015: Histology and Histopathology
https://www.readbyqxmd.com/read/26135030/modeling-of-hematologic-malignancies-by-ips-technology
#17
REVIEW
Shunya Arai, Masashi Miyauchi, Mineo Kurokawa
Induced pluripotent stem cells (iPSCs) can be generated from various types of cells with transduction of defined transcription factors. Patient-derived iPSCs are becoming commonly utilized for understanding the molecular pathways involved in disease and for the development of novel targeted therapies. With the use of patient-derived iPSCs differentiated to specific-lineage cells, the potency and toxicity of drug candidates can be evaluated. In the past, patient-derived iPSCs were mainly established from patients of inherited hematologic diseases, followed by the expansion of target to acquired diseases like myeloproliferative neoplasms...
August 2015: Experimental Hematology
https://www.readbyqxmd.com/read/25956855/role-of-zscan4-in-secondary-murine-ipsc-derivation-mediated-by-protein-extracts-of-esc-or-ipsc
#18
Yoo-Wook Kwon, Jae-Seung Paek, Hyun-Jai Cho, Choon-Soo Lee, Ho-Jae Lee, In-Hyun Park, Tae-Young Roh, Chang-Mo Kang, Han-Mo Yang, Young-Bae Park, Hyo-Soo Kim
Previously, we found that the delivery of mouse ES (mES) cell-derived proteins to adult fibroblasts enables the full reprogramming of these cells, converting them to mouse pluripotent stem cells (protein-iPS cells) without transduction of defined factors. During reprogramming, global gene expression and epigenetic status such as DNA methylation and histone modifications convert from somatic to ES-equivalent status. mES cell extract-derived iPS cells are biologically and functionally indistinguishable from mES cells in its potential in differentiation both in vitro and in vivo...
August 2015: Biomaterials
https://www.readbyqxmd.com/read/25948233/-research-and-application-of-ips-cells-in-blood-system
#19
REVIEW
Li-Xia Zhou, Jie-Yu Ye, Qi-Zhou Lian, Mo Yang
Induced pluripotent stem cells (iPS cells) were first constructed by Takahshi and et al in 2006. They converted the mouse fibroblasts into ES-like cells via viral transduction with four transcription factors (Oct4, Sox2, Klf4 and c-Myc). Since, the significant progress has been made and many researchers have succeeded in inducing iPS cells from other human somatic cells by some novel approaches, such as combining transcriptional factors and small chemicals. IPS cells have significant prospect in clinical application...
April 2015: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/25907774/gene-expression-of-oct4-sox2-klf4-and-myc-oskm-induced-pluripotent-stem-cells-identification-for-potential-mechanisms
#20
Yanning Cai, Xianhua Dai, Qianhua Zhang, Zhiming Dai
BACKGROUND: Somatic cells could be reprogrammed to induced pluripotent stem cells (iPS) by ectopic expression of OCT4, SOX2, KLF4 and MYC (OSKM). We aimed to gain insights into the early mechanisms underlying the induction of pluripotency. METHODS: GSE28688 containing 14 gene expression profiles were downloaded from GEO, including untreated human neonatal foreskin fibroblasts (HFF1) as control, OSKM-induced HFF1 (at 24, 48, 72 h post-transduction of OSKM encoding viruses), two iPS cell lines, and two embryonic stem (ES) cell lines...
2015: Diagnostic Pathology
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