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IPS TRANSDUCTION

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https://www.readbyqxmd.com/read/29805109/generation-of-tcr-expressing-innate-lymphoid-like-helper-cells-that-induce-cytotoxic-t-cell-mediated-anti-leukemic-cell-response
#1
Norihiro Ueda, Yasushi Uemura, Rong Zhang, Shuichi Kitayama, Shoichi Iriguchi, Yohei Kawai, Yutaka Yasui, Minako Tatsumi, Tatsuki Ueda, Tian-Yi Liu, Yasutaka Mizoro, Chihiro Okada, Akira Watanabe, Mahito Nakanishi, Satoru Senju, Yasuharu Nishimura, Kiyotaka Kuzushima, Hitoshi Kiyoi, Tomoki Naoe, Shin Kaneko
CD4+ T helper (Th) cell activation is essential for inducing cytotoxic T lymphocyte (CTL) responses against malignancy. We reprogrammed a Th clone specific for chronic myelogenous leukemia (CML)-derived b3a2 peptide to pluripotency and re-differentiated the cells into original TCR-expressing T-lineage cells (iPS-T cells) with gene expression patterns resembling those of group 1 innate lymphoid cells. CD4 gene transduction into iPS-T cells enhanced b3a2 peptide-specific responses via b3a2 peptide-specific TCR...
June 5, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29721022/reprogramming-of-mouse-calvarial-osteoblasts-into-induced-pluripotent-stem-cells
#2
Yinxiang Wang, Jessica Aijia Liu, Keith K H Leung, Mai Har Sham, Danny Chan, Kathryn S E Cheah, Martin Cheung
Previous studies have demonstrated the ability of reprogramming endochondral bone into induced pluripotent stem (iPS) cells, but whether similar phenomenon occurs in intramembranous bone remains to be determined. Here we adopted fluorescence-activated cell sorting-based strategy to isolate homogenous population of intramembranous calvarial osteoblasts from newborn transgenic mice carrying both Osx1-GFP::Cre and Oct4-EGFP transgenes. Following retroviral transduction of Yamanaka factors (Oct4, Sox2, Klf4, and c-Myc), enriched population of osteoblasts underwent silencing of Osx1-GFP::Cre expression at early stage of reprogramming followed by late activation of Oct4-EGFP expression in the resulting iPS cells...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29321585/site-specific-randomization-of-the-endogenous-genome-by-a-regulatable-crispr-cas9-piggybac-system-in-human-cells
#3
Kentaro Ishida, Huaigeng Xu, Noriko Sasakawa, Mandy Siu Yu Lung, Julia Alexandra Kudryashev, Peter Gee, Akitsu Hotta
Randomized mutagenesis at an endogenous chromosomal locus is a promising approach for protein engineering, functional assessment of regulatory elements, and modeling genetic variations. In mammalian cells, however, it is challenging to perform site-specific single-nucleotide substitution with single-stranded oligodeoxynucleotide (ssODN) donor templates due to insufficient homologous recombination and the infeasibility of positive selection. Here, we developed a DNA transposon based CRISPR-Cas9 regulated transcription and nuclear shuttling (CRONUS) system that enables the stable transduction of CRISPR-Cas9/sgRNA in broad cell types, but avoids undesired genome cleavage in the absence two chemical inducing molecules...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29260008/functional-validation-and-expression-analysis-of-myotubes-converted-from-skin-fibroblasts-using-a-simple-direct-reprogramming-strategy
#4
Fukuko Horio, Hidetoshi Sakurai, Yutaka Ohsawa, Shiho Nakano, Makoto Matsukura, Isao Fujii
Previously, we reported that MyoD, a master gene for myogenic cells, could efficiently convert primary skin fibroblasts into myoblasts and myotubes, thereby effecting direct reprogramming. In this study, we further demonstrated that MyoD-expressing primary fibroblasts displayed rapid movement in culture, with a movement velocity that was significantly faster, almost four times, than mouse primary myoblasts. MyoD-transduced cells obtained the characteristics of Ca2 + release and electrically-stimulated contraction, which was comparable to C2C12 myotubes, suggesting that the essential features of muscle were observed in the transduced cells...
March 2017: ENeurologicalSci
https://www.readbyqxmd.com/read/29228630/transplantation-of-ips-cell-derived-neural-progenitors-overexpressing-sdf-1%C3%AE-increases-regeneration-and-functional-recovery-after-ischemic-stroke
#5
Monica Chau, Todd C Deveau, Mingke Song, Zheng Z Wei, Xiaohuan Gu, Shan Ping Yu, Ling Wei
Ischemic stroke is a leading cause of human death and disability while clinical treatments are limited. The adult brain possesses endogenous regenerative activities that may benefit tissue repair after stroke. Trophic factors such as stromal cell-derived factor 1 alpha (SDF-1α) are upregulated in the ischemic brain, which promote endogenous regeneration. The regenerative response, however, is normally insufficient. Transplantation of exogenous cells has been explored as regenerative therapies. One promising cell type for transplantation is induced pluripotent stem (iPS) cells which are cells genetically reprogrammed from adult somatic cells...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29212797/-in-vitro-and-in-vivo-differentiation-of-induced-pluripotent-stem-cells-generated-from-urine-derived-cells-into-cardiomyocytes
#6
Yu-Feng Jiang, Min Chen, Nan-Nan Zhang, Hua-Jia Yang, Qing Rui, Ya-Feng Zhou
Generation of human cardiomyocytes from cells derived from various sources, including skin biopsy, has been made possible by breakthrough advances in stem cell research. However, it is attractive to build up a negligibly invasive way to create induced pluripotent stem (iPS) cells. In this study, we created iPS cells from human urine-derived epithelial cells by gene transduction using lentiviral vectors in a totally noninvasive manner. Then, we induced the differentiation of iPS cells into functional cardiomyocytes both in vitro and in vivo Action potentials were recorded in putative cardiomyocytes and spontaneous beating cells were observed...
January 8, 2018: Biology Open
https://www.readbyqxmd.com/read/29192290/direct-conversion-of-human-fibroblasts-into-hepatocyte-like-cells-by-atf5-prox1-foxa2-foxa3-and-hnf4a-transduction
#7
Daiki Nakamori, Hiroki Akamine, Kazuo Takayama, Fuminori Sakurai, Hiroyuki Mizuguchi
Recently, it has been reported that human hepatocyte-like cells can be generated from fibroblasts by direct reprogramming technology. However, the conversion efficiency of human induced hepatocyte-like cells (hiHeps) is not high enough. In addition, comparative analysis with the existing models of hepatocytes, such as human iPS cell-derived hepatocyte-like cells and primary human hepatocytes, has not been sufficiently carried out. In this study, we screened hepatic transcription factors for efficient direct hepatic reprogramming and compared hepatic functions between hiHeps and other existing hepatocyte models...
November 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29118915/a-novel-culture-system-robustly-maintained-pluripotency-of-embryonic-stem-cells-and-accelerated-somatic-reprogramming-by-activating-wnt-signaling
#8
Shaojie Li, Xiao Lu, Haipeng He, Rong Cui, Xianxin Wang, Xiaoyun Wang, Xia Wu
Wnt signaling is intrinsic to embryonic stem cell self-renewal and mammalian development. However, the effects of wnts on ES cells self-renewal and iPS cells transduction was not clearly understood. In this study, L-Wnt3a cells that secreted activated Wnt3a protein into medium were used to produce Wnt3a condition medium (Wnt3a-CM) or feeder layer for ES cells cultivation and iPS cells transduction. The results showed that L-Wnt3a cells as feeder layer significantly promoted establishment of ES cell lines and generation of iPS cells...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29111375/a-novel-protocol-to-provide-a-suitable-cardiac-model-from-induced-pluripotent-stem-cells
#9
Mahsa Rasekhi, Masoud Soleimani, Behnaz Bakhshandeh, Majid Sadeghizadeh
Cumulative evidence has proven the safety, feasibility and efficacy of stem cell therapy for cardiomyocyte replacement in heart failure treatment. In contrast to embryonic stem cells, induced pluripotent stem cells (iPS cells) provide a route to the production of patient-specific stem cell lines with no ethical concerns. Recent studies have revealed that myogenic transcription factors activated the expression of conserved microRNAs (miRNAs), such as mir-1, that 'fine-tuned' the output of the transcriptional networks...
November 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/29093090/inducible-microrna-3570-feedback-inhibits-the-rig-i-dependent-innate-immune-response-to-rhabdovirus-in-teleost-fish-by-targeting-mavs-ips-1
#10
Tianjun Xu, Qing Chu, Junxia Cui, Dekun Bi
Effectively recognizing invading viruses and subsequently inducing innate antiviral immunity are essential for host antiviral defense. Although these processes are closely regulated by the host to maintain immune balance, viruses have evolved the ability to downregulate or upregulate these processes for their survival. MicroRNAs (miRNAs) are a family of small noncoding RNAs that play vital roles in modulating host immune response. Accumulating evidence demonstrates that host miRNAs as mediators are involved in regulating viral replication and host antiviral immunity in mammals...
January 15, 2018: Journal of Virology
https://www.readbyqxmd.com/read/28654887/glucose-oxidase-initiated-cascade-catalysis-for-sensitive-impedimetric-aptasensor-based-on-metal-organic-frameworks-functionalized-with-pt-nanoparticles-and-hemin-g-quadruplex-as-mimicking-peroxidases
#11
Xingxing Zhou, Shijing Guo, Jiaxi Gao, Jianmin Zhao, Shuyan Xue, Wenju Xu
Based on cascade catalysis amplification driven by glucose oxidase (GOx), a sensitive electrochemical impedimetric aptasensor for protein (carcinoembryonic antigen, CEA as tested model) was proposed by using Cu-based metal-organic frameworks functionalized with Pt nanoparticles, aptamer, hemin and GOx (Pt@CuMOFs-hGq-GOx). CEA aptamer loaded onto Pt@CuMOFs was bound with hemin to form hemin@G-quadruplex (hGq) with mimicking peroxidase activity. Through sandwich-type reaction of target CEA and CEA aptamers (Apt1 and Apt2), the obtained Pt@CuMOFs-hGq-GOx as signal transduction probes (STPs) was captured to the modified electrode interface...
December 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28447889/bone-marrow-as-a-hematopoietic-stem-cell-source-for-gene-therapy-in-sickle-cell-disease-evidence-from-rhesus-and-scd-patients
#12
Naoya Uchida, Atsushi Fujita, Matthew M Hsieh, Aylin C Bonifacino, Allen E Krouse, Mark E Metzger, Robert E Donahue, John F Tisdale
Steady state bone marrow (BM) is the preferred hematopoietic stem cell (HSC) source for gene therapy in sickle cell disease (SCD) due to the recognized risk of vaso-occlusive crisis during granulocyte colony-stimulating factor mobilization. We previously established clinically relevant HSC gene transfer in the rhesus model following transplantation of mobilized peripheral blood (PB) CD34+ cells transduced with lentiviral vectors. In this study, we examined steady state bone marrow (BM) in the rhesus competitive repopulation model and demonstrate similar gene marking in vitro and in vivo, as compared with mobilized PB CD34+ cells...
September 2017: Human Gene Therapy. Clinical Development
https://www.readbyqxmd.com/read/28413817/dataset-in-support-of-the-generation-of-niemann-pick-disease-type-c1-patient-specific-ips-cell-lines-carrying-the-novel-npc1-mutation-c-1180t-c-or-the-prevalent-c-3182t-c-mutation-analysis-of-pluripotency-and-neuronal-differentiation
#13
Franziska Peter, Michaela Trilck, Michael Rabenstein, Arndt Rolfs, Moritz J Frech
Data presented in this article demonstrate the generation and characterization of two novel Niemann-Pick disease Type C1 (NPC1) patient-specific induced pluripotent stem cell (iPSC) lines, related to the research article Trilck et al. (Diversity of Glycosphingolipid GM2 and Cholesterol Accumulation in NPC1 Patient-Specific iPSC-Derived Neurons; Brain Res.; 2017; 1657:52-61. doi: 10.1016/j.brainres.2016.11.031). For reprogramming fibroblasts, carrying the novel homozygous mutation c.1180T>C and the prevalent homozygous mutation c...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28393187/generation-and-characterization-of-induced-pluripotent-stem-cells-from-guinea-pig-fetal-fibroblasts
#14
Yuehong Wu, Ouyang Li, Chengwen He, Yong Li, Min Li, Xiaoming Liu Liu, Yujiong Wang, Yulong He
Induced pluripotent stem cells (iPS) represent an important tool to develop disease‑modeling assays, drug testing assays and cell‑based replacement therapies. The application of iPS in these fields requires the development of suitable animal models. Of the suitable species, guinea pigs are particularly important and offer significant advantages. Successful iPS generation has been accomplished in a number of species; however, it has not been reported in the guinea pig. The present study successfully generated iPS from guinea pigs (giPS) using single polycistronic virus transduction with mouse octamer‑binding transcription factor 4 (Oct4), sex determining region Y‑box 2 (Sox2), Kruppel‑like factor 4 and c‑Myc...
June 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28179988/ips-derived-mscs-from-an-expandable-bank-to-deliver-a-prodrug-converting-enzyme-that-limits-growth-and-metastases-of-human-breast-cancers
#15
M Ullah, Y Kuroda, T J Bartosh, F Liu, Q Zhao, C Gregory, R Reger, J Xu, R H Lee, D J Prockop
One attractive strategy to treat cancers is to deliver an exogenous enzyme that will convert a non-toxic compound to a highly toxic derivative. The strategy was tested with viral vectors but was disappointing because the efficiency of transduction into tumor cells was too low. Recent reports demonstrated that the limitation can be addressed by using tissue-derived mesenchymal stromal cells (MSCs) to deliver enzyme/prodrug systems that kill adjacent cancer cells through bystander effects. Here we addressed the limitation that tissue-derived MSCs vary in their properties and are difficult to generate in the large numbers needed for clinical applications...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/27900567/clinical-potential-of-human-induced-pluripotent-stem-cells-perspectives-of-induced-pluripotent-stem-cells
#16
REVIEW
Dharmendra Kumar, Taruna Anand, Wilfried A Kues
The recent establishment of induced pluripotent stem (iPS) cells promises the development of autologous cell therapies for degenerative diseases, without the ethical concerns associated with human embryonic stem (ES) cells. Initially, iPS cells were generated by retroviral transduction of somatic cells with core reprogramming genes. To avoid potential genotoxic effects associated with retroviral transfection, more recently, alternative non-viral gene transfer approaches were developed. Before a potential clinical application of iPS cell-derived therapies can be planned, it must be ensured that the reprogramming to pluripotency is not associated with genome mutagenesis or epigenetic aberrations...
April 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/27856246/reprogramming-of-retinoblastoma-cancer-cells-into-cancer-stem-cells
#17
Fengming Yue, Kanji Hirashima, Daihachiro Tomotsune, Sakiko Takizawa-Shirasawa, Tadayuki Yokoyama, Katsunori Sasaki
Retinoblastoma is the most common intraocular malignancy in pediatric patients. It develops rapidly in the retina and can be fatal if not treated promptly. It has been proposed that a small population of cancer cells, termed cancer stem cells (CSCs), initiate tumorigenesis from immature tissue stem cells or progenitor cells. Reprogramming technology, which can convert mature cells into pluripotent stem cells (iPS), provides the possibility of transducing malignant cancer cells back to CSCs, a type of early stage of cancer...
January 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27826858/analysis-of-protein-protein-interactions-between-men-components
#18
Rami Rahal
The isolation of protein complexes is a powerful method to elucidate how signaling networks are regulated. Co-immunoprecipitation (co-IP) is a widely used procedure to detect and characterize the physical association of pathway components that has frequently been used to reveal mechanisms of pathway regulation. This chapter outlines the roles of protein-protein interactions in signal transduction and the regulatory functions such assemblies impart to cells. The principles behind co-IPs are discussed and best practices for conducting and interpreting co-IPs experiments are outlined...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27788138/towards-a-safer-more-randomized-lentiviral-vector-integration-profile-exploring-artificial-ledgf-chimeras
#19
Lenard S Vranckx, Jonas Demeulemeester, Zeger Debyser, Rik Gijsbers
The capacity to integrate transgenes into the host cell genome makes retroviral vectors an interesting tool for gene therapy. Although stable insertion resulted in successful correction of several monogenic disorders, it also accounts for insertional mutagenesis, a major setback in otherwise successful clinical gene therapy trials due to leukemia development in a subset of treated patients. Despite improvements in vector design, their use is still not risk-free. Lentiviral vector (LV) integration is directed into active transcription units by LEDGF/p75, a host-cell protein co-opted by the viral integrase...
2016: PloS One
https://www.readbyqxmd.com/read/27572712/induced-pluripotent-stem-ips-cells-in-dentistry-a-review
#20
REVIEW
Neeraj Malhotra
iPS cells are derived from somatic cells via transduction and expression of selective transcription factors. Both viral-integrating (like retroviral) and non-integrating (like, mRNA or protein-based) techniques are available for the production of iPS cells. In the field of dentistry, iPS cells have been derived from stem cells of apical papilla, dental pulp stem cells, and stem cells from exfoliated deciduous teeth, gingival and periodontal ligament fibroblasts, and buccal mucosa fibroblasts. iPS cells have the potential to differentiate into all derivatives of the 3 primary germ layers i...
November 30, 2016: International Journal of Stem Cells
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