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https://www.readbyqxmd.com/read/28637587/comparative-study-on-secretome-and-transmembranome-of-immature-and-mature-metacestodes-of-echinococcus-multilocularis
#1
Fuqiang Huang, Zhisheng Dang, Haoji Zhang, Kinpei Yagi, Kyeongsoon Kim, Magona Joseph, Wenjun Pu, Yuzaburo Oku
Alveolar echinococcosis (AE) is a worldwide zoonosis caused by E. multilocularis. Humans become infected through oral ingestion of the eggs. Host of E. multilocularis produces immune responses that help to either reject and/or limit the growth of this parasite, and in response the parasite produces molecules against this immune attack. This study identifies candidate key molecules in the early infection phase and the chronic stage of the parasite infestation, through comparison of gene expression of 4- and 16-week metacestodes...
May 12, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28630663/presentation-of-secondary-parasitic-infection-37-years-after-primary-infection
#2
Gary Sharp, David Yeo, Cherry Koh
Echinococcus granulosus (EG) is a neglected pathology that causes cystic echinococcosis and primarily affects the liver and lung. EG infects ~6 million worldwide and mortality is quoted as 2-4% per 100 000 inhabitants. The increase in human traffic from endemic regions demands clinician's awareness. Dogs are the most common definitive host for the EG tapeworm. Human infection requires ingestion of fecal parasitic eggs. Primary infection causes cysts to appear in affected organs, rupture of which leads to secondary infection...
June 2017: Journal of Surgical Case Reports
https://www.readbyqxmd.com/read/28630383/balb-c-mice-immunity-to-hydatidosis-induced-by-in-vitro-reared-echinococcus-granulosus-adult-worm-antigens
#3
Hamid Reza Rahimi, Tahereh Mohammadzadeh, Seyed Mahmoud Sadjjadi, Bahador Sarkari, Farzaneh Zahabiun
BACKGROUND: Echinococcosis is a zoonotic parasitic disease caused by the larval stage of Echinococcus granulosus. Several native and recombinant antigens, derived from different stages of E. granulosus life cycle, have been used for vaccine trials. In vitro reared adult worms are good candidates for vaccination as they do not produce fertile egg/s and do not have any risk of contamination for researchers. OBJECTIVE: To evaluate different antigens derived from in vitro reared E...
June 2017: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/28630382/immunodiagnostic-value-of-echinococcus-granulosus-recombinant-b8-1-subunit-of-antigen-b
#4
Amir Savardashtaki, Bahador Sarkari, Farzane Arianfar, Zohreh Mostafavi-Pour
BACKGROUND: Cystic echinococcosis (CE), as a chronic parasitic disease, is a major health problem in many countries. The performance of the currently available serodiagnostic tests for the diagnosis of CE is unsatisfactory. OBJECTIVE: The current study aimed at sub-cloning a gene, encoding the B8/1 subunit of antigen B (AgB) from Echinococcus granulosus, using gene optimization for the immunodiagnosis of human CE. METHODS: The coding sequence for AgB8/1 subunit of Echinococcus granulosus was selected from GenBank and was gene-optimized...
June 2017: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/28624512/epidemiological-investigation-and-risk-factors-of-echinococcus-granulosus-in-yaks-bos-grunniens-tibetan-pigs-and-tibetans-on-qinghai-tibetan-plateau
#5
Kun Li, Lihong Zhang, Hui Zhang, Zhixin Lei, Houqiang Luo, Khalid Mehmood, Muhammad Shahzad, Yanfang Lan, Meng Wang, Jiakui Li
Echinococcus granulosus (E. granulosus) is a diverse zoonotic parasite and causes Cystic echinococcosis (CE) disease in humans and livestock. However, scare information is available about the epidemic situation of E. granulosus infection in yaks, Tibetan pigs and native Tibetans on the Qinghai Tibetan plateau. Therefore, a study was carried out to find prevalence and risk factors of E. granulosus in yaks, Tibetan pigs and Tibetans. Serum samples from yaks (1371), Tibetan pigs (454) and Tibetans (600) were collected and assessed by commercial ELISA kits...
June 15, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28621034/depletion-of-foxp3-tregs-improves-control-of-larval-echinococcus-multilocularis-infection-by-promoting-co-stimulation-and-th1-17-immunity
#6
Junhua Wang, Stephan Müller, Renyong Lin, Myriam Siffert, Dominique A Vuitton, Hao Wen, Bruno Gottstein
INTRODUCTION: The growth potential of the tumor-like Echinococcus multilocularis metacestode (causing alveolar echinococcosis, AE) is directly linked to the nature/function of the periparasitic host immune-mediated processes. Previous studies had shown that regulatory T cells (Tregs) become gradually up-regulated in the course of both chronic human and murine AE. Thus we now tackled the role of FoxP3(+) Tregs and FoxP3(+) -Treg-regulated immune response in contributing to the control of this helminthic infection...
June 16, 2017: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/28617666/parasitic-diseases-of-camels-in-iran-1931-2017-a-literature-review
#7
Alireza Sazmand, Anja Joachim
Parasitic diseases of camels are major causes of impaired milk and meat production, decreases in performance or even death. Some camel parasites also represent a threat to human health. About 171,500 one-humped camels (Camelus dromedarius) and 100-300 two-humped camels (Camelus bactrianus) live in Iran. Knowledge of the biodiversity of their parasites is still limited. The present review covers all information about camel parasitic diseases in Iran published as dissertations and in both Iranian and international journals from 1931 to February 2017...
2017: Parasite: Journal de la Société Française de Parasitologie
https://www.readbyqxmd.com/read/28615839/epidemiological-and-clinical-aspects-of-patients-with-hydatid-cyst-in-iran
#8
Mahmood Moosazadeh, Ghasem Abedi, Seif Ali Mahdavi, Jalil Shojaee, Ali Charkame, Mahdi Afshari
Hydatid cyst is one of the most common Zoonotic diseases occurs due to infection with larval stage of Echinococcus granulosus parasite. Based on epidemiological reasons, World Health Organization has introduced Iran as a hyperendemic region. This study aims to investigate the clinico-epidemiological characteristics of patients with hydatid cyst in Iran. We used information registered in the surveillance system of the Iranian Ministry of Health and Medical Education attributed to Mazandaran province. Data were entered into SPSS software V...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28607793/the-case-of-reactive-arthritis-secondary-to-echinococcus-infestation
#9
Bülent Alım, Sinan Çetinel, M Alperen Servi, Fahrettin Bostancı, Mehmet Ozan Bingöl
Reactive arthritis is an inflammatory joint disease that develops after an infection and it usually occurs following a gastrointestinal or genitourinary system infection and it belongs to the family of arthritis called "spondyloarthritis." We wanted to represent a rare case of reactive arthritis secondary to Echinococcus infestation. Cyst hydatid disease is common in endemic regions like Turkey. Internal organ involvements, especially liver and lung, are most frequent involvements. Primary bone involvement is rare complication of Echinococcus infestation...
2017: Case Reports in Rheumatology
https://www.readbyqxmd.com/read/28606323/coprodiagnosis-of-echinococcus-granulosus-infection-in-dogs-from-ankara-turkey
#10
Hatice Öge, Semih Öge, Bahadır Gönenç, Oğuz Sarımehmetoğlu, Gökben Özbakış
The present study were undertaken to compare two isolation techniques (centrifugal flotation and sedimentation) for recovering taeniid eggs from faecal samples, to identify E. granulosus DNA from taeniid eggs by PCR, and to determine the prevalence of E. granulosus in dogs in villages. Faecal samples were collected from 100 dogs in Ankara province. Taenia spp. eggs were found in 27% of dogs faeces. Echinococcus granulosus-specific PCR was obtained in 14 (51.85%) of the taeniid eggs-positive samples. As well as finding Taenia eggs in dogs' faeces, we also found eggs of some helminthic parasites; such as Dipylidium caninum, Toxocara canis, Toxascaris leonina, Trichuris sp...
August 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28606321/characterization-of-glutathione-s-transferase-and-its-immunodiagnostic-potential-for-detecting-taenia-multiceps
#11
Ying Sun, Yu Wang, Xing Huang, Xiaobing Gu, Weimin Lai, Xuerong Peng, Guangyou Yang
Taenia multiceps is a widespread zoonotic tapeworm parasite which infects cloven-hoofed animals around the world. Animal infection with Coenurus cerebralis, the coenurus larvae of T. multiceps (Tm), is often fatal, which is a major cause of economic losses in stockbreeding. This study amplified the glutathione S-transferase (GST) gene from the total RNA of C. cerebralis. The resulting protein, Tm-GST, consisted of 201 amino acids, and had a predicted molecular mass of 23.1kDa. Its amino acid sequence shares 77...
August 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28599696/primary-sacral-hydatid-cyst-causing-cutaneous-fistula
#12
Volkan Murat Unal, Nail Ozdemir, Ali Karadag, Serdar Oguzoglu, Haydar Celik
Echinococcus granulosus and Echinococcus multilocularis are the causes of hydatid disease and the main characteristic is endemic. Generally, it affects the liver and lungs. Spinal hydatidosis accounts for less than 1% of the cases. Vertebral hydatidosis is usually silent and a slowly progressive disease with a long latent period. Another rare form is the primary sacral hydatid cyst. Generally, patients suffer from back pain and neural compression symptoms. A 43-year woman was admitted with left leg pain and a fluid leakage from a cutaneous fistula on the left hip...
May 2017: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/28593390/origin-differentiation-and-functional-ultrastructure-of-egg-envelopes-in-the-cestode-echinococcus-multilocularis-leuckart-1863-cyclophyllidea-taeniidae
#13
Zdzisław Świderski, Jordi Miquel, Samira Azzouz-Maache, Anne-Françoise Pétavy
The origin, differentiation and functional ultrastructure of oncospheral or egg envelopes in Echinococcus multilocularis Leuckart, 1863 were studied by transmission electron microscopy (TEM) and cytochemistry. The purpose of our study is to describe the formation of the four primary embryonic envelopes, namely vitelline capsule, outer envelope, inner envelope and oncospheral membrane, and their transformation into the oncospheral or egg envelopes surrounding the mature hexacanth. This transformation takes place in the preoncospheral phase of embryonic development...
July 2017: Parasitology Research
https://www.readbyqxmd.com/read/28589871/prevalence-of-cystic-echinococcosis-in-iran-a-systematic-review-and-meta-analysis
#14
H R Khalkhali, M Foroutan, S Khademvatan, H Majidiani, S Aryamand, P Khezri, A Aminpour
As a significant zoonosis, cystic echinococcosis (CE) is endemic in some parts of the world, such as the Middle East. There are studies on the prevalence of this infection in animal and human reservoirs in Iran; hence, we conducted this meta-analysis to elucidate the prevalence of CE in Iran. English (PubMed, Scopus, Web of Science, Science Direct and Google Scholar) and Persian (Magiran, Iran Medex, Iran Doc and SID) databases were explored. In the case of definitive, animal and human intermediate hosts, 37, 90 and 33 studies, respectively, have been included in the current review from January 1990 to December 2015...
June 7, 2017: Journal of Helminthology
https://www.readbyqxmd.com/read/28588571/genome-wide-transcriptome-analysis-reveals-extensive-alternative-splicing-events-in-the-protoscoleces-of-echinococcus-granulosus-and-echinococcus-multilocularis
#15
Shuai Liu, Xiaosu Zhou, Lili Hao, Xianyu Piao, Nan Hou, Qijun Chen
Alternative splicing (AS), as one of the most important topics in the post-genomic era, has been extensively studied in numerous organisms. However, little is known about the prevalence and characteristics of AS in Echinococcus species, which can cause significant health problems to humans and domestic animals. Based on high-throughput RNA-sequencing data, we performed a genome-wide survey of AS in two major pathogens of echinococcosis-Echinococcus granulosus and Echinococcus multilocularis. Our study revealed that the prevalence and characteristics of AS in protoscoleces of the two parasites were generally consistent with each other...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28584847/acute-appendicitis-caused-by-an-echinococcal-brood-capsule-unmasks-an-asymptomatic-hepatic-hydatid-cyst
#16
Krishnendu Mondal, Rupali Mandal
Hydatidosis is an endemic helminthic disease in the cattle-grazing regions of Asia. It is usually caused by the cestode Echinococcus granulosus. Internal organs, particularly the liver and lungs, are predominantly affected, but the appendix is only rarely involved with the formation of characteristic hydatid cyst. We present a unique case of appendiceal hydatidosis in an 18-year-old woman with acute appendicitis. Her preoperative abdominal ultrasound revealed an asymptomatic hydatid cyst in the liver. An echinococcal brood capsule was detected postoperatively within the appendicular lumen under microscope...
2017: ACG Case Reports Journal
https://www.readbyqxmd.com/read/28576342/development-of-a-colloidal-gold-immunochromatographic-strip-based-on-hsp70-for-the-rapid-detection-of-echinococcus-granulosus-in-sheep
#17
Xunhui Zhuo, Yingchao Yu, Xueqiu Chen, Zhuangzhi Zhang, Yi Yang, Aifang Du
Echinococcus granulosus is the causative pathogen of cystic echinococcosis, a serious disease endangering human and animal health. In this study, an immunochromatographic strip was developed based on the recombinant protein Heat shock protein 70 (HSP70) for the serological detection of E. granulosus. The protocol completes within 20min requiring no specialized equipment or chemical reagents, while specificity tests confirmed no cross-reactivity with positive serum of Fasciola hepatica, Haemonchus contortus, Peste des petits ruminants virus (PPRV) and Foot-and-mouth disease virus (FMDV)...
June 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28567559/diagnosis-of-canine-echinococcus-multilocularis-infections-by-copro-dna-tests-comparison-of-dna-extraction-techniques-and-evaluation-of-diagnostic-deworming
#18
Takao Irie, Takuya Ito, Hirokazu Kouguchi, Kimiaki Yamano, Kohji Uraguchi, Kinpei Yagi, Nariaki Nonaka
The use of copro-DNA detection methods for the diagnosis of canine Echinococcus multilocularis infection was evaluated with a focus on DNA extraction techniques: two commercial kits and a modified alkaline-sodium dodecyl sulfate (SDS) technique. Dog feces (0.2 g) mixed with a protoscolex or with 1 or 10 eggs of E. multilocularis were subjected to DNA detection following extraction by these methods. DNA was extracted from all protoscolex samples by all methods, but success for samples with eggs depended on extraction technique with the modified technique showing success on all samples...
May 31, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28559529/tubercular-infection-of-hydatid-cyst
#19
Tushar Patial, Gopal Singh, Digvijay Thakur, Sahil Chaddha
Tubercular infection of hydatid cyst of the chest wall in an immunocompetent individual is rare. Immune modulation for symbiosis between host cells and the parasite - Echinococcus granulosus favors tubercular infection. In this case report, we describe a case of both these chronic diseases coexisting together, to present as chest wall mass.
April 2017: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28558809/genetic-variation-of-mitochondrial-genes-among-echinococcus-multilocularis-isolates-collected-in-western-china
#20
Chuanchuan Wu, Wenbao Zhang, Bo Ran, Haining Fan, Hui Wang, Baoping Guo, Canlin Zhou, Yingmei Shao, Wei Zhang, Patrick Giraudoux, Jenny Knapp, Hao Wen, Ling Kuang, Jun Li
BACKGROUND: Alveolar echinococcosis (AE) is a life-threatening human disease caused by Echinococcus multilocularis transmitted between rodents and dogs/foxes in the Northern Hemisphere. The study aims to identify the genetic variation of the parasite in AE patients from China. METHODS: E. multilocularis isolates were collected from wild small mammals (n = 6) and AE patients (n = 56) from western China. Genomic DNA was extracted from different tissue samples including paraffin tissue blocks, ethanol fixed tissues and frozen tissues surgically removed...
May 30, 2017: Parasites & Vectors
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