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https://www.readbyqxmd.com/read/28096852/echinococcus-granulosus-sensu-stricto-in-dogs-and-jackals-from-caspian-sea-region-northern-iran
#1
Shirzad Gholami, Hefzallah Jahandar, Mahdi Abastabar, Abdolsatar Pagheh, Iraj Mobedi, Mitra Sharbatkhori
BACKGROUND: The aim of the present study was genotyping of Echinococcus granulosus isolates from dogs and jackals in Mazandaran Province, northern Iran, and using partial sequence of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1). METHODS: E. granulosus isolates (n = 15) were collected from 42 stray dogs and 16 jackals found in south of the Caspian Sea in northern Iran. After morphological study, the isolates were genetically characterized using consensus sequences (366bp) of the cox1 gene...
April 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28096605/molecular-and-epidemiological-updates-on-cystic-echinococcosis-infecting-water-buffaloes-from-egypt
#2
Ibrahim Abbas
AIM: Cystic echinococcosis (CE) represents a serious parasitic disease at both animal and public health levels. The majority of reports negated the CE infection in buffaloes from Egypt; however, one study illustrated their infection with G6 genotype (camel strain). The present work contributed to update the epidemiological and molecular knowledge about CE infecting this economically important animal for better understanding of its role in maintaining the Echinococcus life cycle. MATERIALS AND METHODS: A total of 120 slaughtered water buffaloes at Mansoura abattoir, Dakahlia province, Egypt, were inspected for the existence of hydatid cysts...
December 2016: Veterinary World
https://www.readbyqxmd.com/read/28095661/molecular-cloning-and-characterization-of-a-p38-like-mitogen-activated-protein-kinase-from-echinococcus-granulosus
#3
Guodong Lü, Jing Li, Chuanshan Zhang, Liang Li, Xiaojuan Bi, Chaowang Li, Jinliang Fan, Xiaomei Lu, Dominique A Vuitton, Hao Wen, Renyong Lin
Cystic echinococcosis (CE) treatment urgently requires a novel drug. The p38 mitogen-activated protein kinases (MAPKs) are a family of Ser/Thr protein kinases, but still have to be characterized in Echinococcus granulosus. We identified a 1,107 bp cDNA encoding a 368 amino acid MAPK protein (Egp38) in E. granulosus. Egp38 exhibits 2 distinguishing features of p38-like kinases: a highly conserved T-X-Y motif and an activation loop segment. Structural homology modeling indicated a conserved structure among Egp38, EmMPK2, and H...
December 2016: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28088247/first-molecular-identification-of-echinococcus-vogeli-and-echinococcus-granulosus-sensu-stricto-g1-revealed-in-feces-of-domestic-dogs-canis-familiaris-from-acre-brazil
#4
Leandro Batista das Neves, Paulo Eduardo Ferlini Teixeira, Sidnei Silva, Fernanda Bittencourt de Oliveira, Daniel Daipert Garcia, Fernanda Barbosa de Almeida, Rosângela Rodrigues-Silva, José Roberto Machado-Silva
BACKGROUND: Echinococcus granulosus (sensu lato) (s.l.) and Echinococcus vogeli are causative agents of chronic zoonotic diseases such as cystic and polycystic echinococcosis, respectively. In Brazil, polycystic echinococcosis has a restricted geographical distribution in the North Region, while cystic echinococcosis is observed in the South Region. Domestic dogs (Canis familiaris) fed with raw viscera represent a risk factor for E. granulosus (s.l.) infection in the South Region. Although this practice is frequent, it remains unclear whether domestic dogs are infected with E...
January 14, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28077161/mathematical-modelling-of-echinococcus-multilocularis-abundance-in-foxes-in-zurich-switzerland
#5
Belen Otero-Abad, Simon R Rüegg, Daniel Hegglin, Peter Deplazes, Paul R Torgerson
BACKGROUND: In Europe, the red fox (Vulpes vulpes) is the main definitive host of Echinococcus multilocularis, the aetiological agent of a severe disease in humans called alveolar echinococcosis. The distribution of this zoonotic parasite among the fox population is remarkably aggregated with few heavily infected animals harbouring much of the parasite burdens and being responsible for most of the environmental parasitic egg contamination. Important research questions explored were: (i) spatial differences in parasite infection pressure related to the level of urbanization; (ii) temporal differences in parasite infection pressure in relation to time of the year; (iii) is herd immunity or an age-dependent infection pressure responsible for the observed parasite abundance; (iv) assuming E...
January 11, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28057995/an-undiagnosed-giant-right-renal-hydatid-cyst-treated-laparoscopically-case-report-and-review-of-literature
#6
Elsawi Osman, Ziauddin Khan, Abdulmenem Abualsel, Tanweer Bhatty
Hydatid disease caused by the tape worm Echinococcus granulosus is a rare occurrence in the urinary system in general. We are hereby presenting a case of a gentleman in his fourth decade with a giant right renal hydatid cyst. The clinical manifestations, radiological features, and serology were all not suggestive of hydatid disease; however, typical Echinococcus scolices were detected histologically following cyst aspiration. The giant cyst was successfully treated laparoscopically.
October 2016: Urology Annals
https://www.readbyqxmd.com/read/28054267/green-chemical-synthesis-of-gold-nanoparticles-by-using-penicillium-aculeatum-and-their-scolicidal-activity-against-hydatid-cyst-protoscolices-of-echinococcus-granulosus
#7
Hamed Barabadi, Soheila Honary, Milad Ali Mohammadi, Ehsan Ahmadpour, Mohammad Taghi Rahimi, Ahad Alizadeh, Farzaneh Naghibi, Muthupandian Saravanan
Hydatid disease is a helminth infection with various clinical complications caused by the larval stage of the dog tapeworm Echinococcus granulosus. The scolicidal agents have been broadly applied for inactivation of the fertile cysts up to now, but these scolicidal agents have several side effects on patients. Therefore, this study aimed to explore the scolicidal activity of green synthesized gold nanoparticles (AuNPs) utilizing mycelia-free culture filtrate of Penicillium aculeatum against hydatid cyst protoscolices of E...
January 4, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28053576/in-vitro-effectiveness-of-curcuma-longa-and-zingiber-officinale-extracts-on-echinococcus-protoscoleces
#8
Esam Almalki, Esam M Al-Shaebi, Saleh Al-Quarishy, Mansour El-Matbouli, Abdel-Azeem S Abdel-Baki
Hydatid disease is an important economic and human public health problem with a wide geographical distribution. Surgical excision remains the primary treatment and the only hope for complete cure of hydatosis. The most important complications arising from surgical excision, however, is recurrence, which is due to dissemination of protoscolices during the surgery. Pre-surgical inactivation of the contents of the hydatid cyst by injection of scolicidal agent into the cyst has been used as adjunct to surgery in order to overcome the risk of recurrence...
January 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28051760/contribution-to-the-diagnosis-and-treatment-of-life-threatening-parasitosis-caused-by-the-parasite-echinococcus-multilocularis
#9
Katarína Šimeková, Mária Szilágyiová, Daniela Antolová, Ladislav Laca, Hubert Polaček, Elena Nováková, Martin Števík, Róbert Rosoľanka
Echinococcosis is a serious parasitic disease that ends lethally in 95% of untreated infected patients. It was first diagnosed in Slovakia in the year 2000. It is caused by the larval stage of a tapeworm belonging to the genus Echinococcus, which was assigned to the group "A" of zoonoses in the year 2004. The number of new infections is rising because of increasing percentage of infected red foxes (Vulpes vulpes). Early and accurate diagnosis of infections with this parasite is essential for proper initiation of adequate therapy...
January 4, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28045899/characterisation-of-antigen-b-protein-species-present-in-the-hydatid-cyst-fluid-of-echinococcus-canadensis-g7-genotype
#10
Ana Maite Folle, Eduardo S Kitano, Analía Lima, Magdalena Gil, Marcela Cucher, Gustavo Mourglia-Ettlin, Leo K Iwai, Mara Rosenzvit, Carlos Batthyány, Ana María Ferreira
The larva of cestodes belonging to the Echinococcus granulosus sensu lato (s.l.) complex causes cystic echinococcosis (CE). It is a globally distributed zoonosis with significant economic and public health impact. The most immunogenic and specific Echinococcus-genus antigen for human CE diagnosis is antigen B (AgB), an abundant lipoprotein of the hydatid cyst fluid (HF). The AgB protein moiety (apolipoprotein) is encoded by five genes (AgB1-AgB5), which generate mature 8 kDa proteins (AgB8/1-AgB8/5). These genes seem to be differentially expressed among Echinococcus species...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28043383/the-putative-serine-protease-inhibitor-serpin-genes-encoded-on-echinococcus-multilocularis-genome-and-their-expressions-in-metacestodal-stage
#11
Mizuki Sasaki, Yasuhito Sako
Two putative serpin genes were identified in Echinococcus multilocularis, in addition to the already reported serpinEmu, and were designated as serpin2Emu and serpin3Emu. Western blot analysis using polyclonal antibodies against serpinEmu, putative serpin2Emu protein, and putative serpin3Emu protein indicated that all three proteins were localized in both intracellular and excretory-secretory (ES) fractions of E. multilocularis metacestodes. In addition, immune staining of parasite tissue indicated that all three proteins were localized at the germinal layer...
January 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28043382/protozoan-and-helminth-parasite-fauna-of-free-living-croatian-wild-wolves-canis-lupus-analyzed-by-scat-collection
#12
Carlos Hermosilla, Sonja Kleinertz, Liliana M R Silva, Jörg Hirzmann, Djuro Huber, Josip Kusak, Anja Taubert
The European wolf (Canis lupus) is a large carnivore species present in limited areas of Europe with several small populations still being considered as endangered. Wolves can be infected by a wide range of protozoan and metazoan parasites with some of them affecting free-living wolf health condition. On this account, an epidemiological survey was conducted to analyze the actual parasite fauna in Croatian wild wolves. In total, 400 individual faecal samples were collected during field studies on wolf ecology in the years 2002-2011...
January 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28035113/serological-surveillance-of-vector-borne-and-zoonotic-diseases-among-hunters-in-eastern-poland
#13
Małgorzata Tokarska-Rodak, Dorota Plewik, Aleksander J Michalski, Marcin Kołodziej, Alicja Mełgieś, Anna Pańczuk, Henryk Konon, Marcin Niemcewicz
BACKGROUND & OBJECTIVES: Many etiological agents of zoonoses are considered as significant biological hazard to people visiting forested areas frequently, for instance, hunters. They may be exposed to ticks, rodents, and birds as well as excreta/secretions of wild animals or contaminated water and soil. Hence, this population is at risk of contracting infection with pathogens such as Borrelia burgdorferi sensu lato (s.l.), Anaplasma phagocytophilum, Babesia spp., tick-borne encephalitis virus, Bartonella spp...
October 2016: Journal of Vector Borne Diseases
https://www.readbyqxmd.com/read/28025118/detection-of-echinococcus-granulosus-and-echinococcus-ortleppi-in-bhutan
#14
Nirmal Kumar Thapa, Maria Teresa Armua-Fernandez, Dukpa Kinzang, Ratna B Gurung, Phuntsho Wangdi, Peter Deplazes
In this pilot study, fecal samples were collected from community dogs around slaughterhouses and from the city of Thimphu (n=138) as well as from carnivores in the forest area around a farm in Bhutan (n=28). Samples were analyzed microscopically for the presence of taeniid eggs by the floatation and sieving method. Further molecular analyses of 20 samples of community dogs positive for taeniid eggs confirmed 10 Echinococcus granulosus sensu lato and one Taenia hydatigena case. From 14 environmental fecal samples from the forest area positive for taeniid eggs, one contained E...
December 24, 2016: Parasitology International
https://www.readbyqxmd.com/read/28017635/use-of-fta-%C3%A2-card-methodology-for-sampling-and-molecular-characterization-of-echinococcus-granulosus-sensu-lato-in-africa
#15
Franck Boué, Ikhlass El Berbri, Vanessa Hormaz, Jean-Marc Boucher, Ahmed Bezeid El Mamy, Abdallah Traore, Ouafaa Fassi Fihri, Anne-Françoise Petavy, Allal Dakkak, Gérald Umhang
Cystic Echinococcosis is a parasitic disease caused by the cestode Echinococcus granulosus widely distributed in Africa. Monitoring of this parasite requires access to cyst samples on intermediate hosts observed at the slaughterhouse. In order to facilitate sampling in the field and analysis, the French National Reference Laboratory for Echinococcus spp. has developed a tissue derived from DNA sampling with FTA(®) card technology. The DNA samples were taken by applying the FTA(®) paper on the germinal layer after opening the cysts...
December 23, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/28012847/echinococcus-granulosus-evidence-of-a-heterodimeric-glutathione-transferase-built-up-by-phylogenetically-distant-subunits
#16
Paula Arbildi, Silvana La-Rocca, Veronica Lopez, Natalia Da-Costa, Veronica Fernandez
In the cestode parasite Echinococcus granulosus, three phylogenetically distant cytosolic glutathione transferases (GSTs) (EgGST1, 2 and 3) were identified. Interestingly, the C-terminal domains of EgGST3 and EgGST2 but not EgGST1, exhibit all amino acids involved in Sigma-class GST dimerization. Here, we provide evidence indicating that EgGST2 and EgGST3 naturally form a heterodimeric structure (EgGST2-3), and also we report the enzymatic activity of the recombinant heterodimer. EgGST2-3 might display novel properties able to influence the infection establishment...
January 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28012251/public-health-follow-up-of-suspected-exposure-to-echinococcus-multilocularis-in-southwestern-ontario
#17
L A Trotz-Williams, N J Mercer, J M Walters, D Wallace, B Gottstein, E Osterman-Lind, A K Boggild, A S Peregrine
In the 3 years since the first report of canine alveolar echinococcosis (AE) in Ontario, three additional cases have been diagnosed in the province. Of the four cases reported to date, three have had no known history of travel outside the province. It is possible that this development is an indication of previously unrecognized environmental contamination with Echinococcus multilocularis eggs in some areas of the province. If so, there is the potential for an emerging threat to human health. This article describes a local public health department's investigation of the possible exposure to E...
December 24, 2016: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28005806/hydatid-cyst-of-the-posterior-cervical-region
#18
Muhsin Nuh Aybay, Ibrahim Guler, Ozgur Oner, Suleyman Baktik, Ali Fuat Tekin
Hydatid cyst is an endemically seen parasitic disease and caused by the larval form of Echinococcus parasites. The disease is mostly seen in liver and lung, and the other involvement is rarely seen. Head and neck involvement is extremely rarely seen and its frequency in the literature is reported as less than 1%. It is usually asymptomatic and the symptoms are related to the compression to the adjacent structures. In this paper, the authors report a patient of hydatid cyst localized in the posterior cervical region...
November 2016: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28002597/characterization-and-application-of-newly-developed-polymorphic-microsatellite-markers-in-the-ezo-red-fox-vulpes-vulpes-schrencki
#19
T Tada, Y Seki, Y Kameyama, Y Kikkawa, K Wada
The Ezo red fox (Vulpes vulpes schrencki), a subspecies endemic to Hokkaido island, Japan, is a known host species for the tapeworm Echinococcus multilocularis. To develop tools for molecular ecological studies, we isolated 28 microsatellite regions from the genome of Ezo red fox, and developed 18 polymorphic microsatellite markers. These markers were characterized using 7 individuals and 22 fecal samples of the Ezo red fox. The number of alleles for these markers ranged from 1 to 7, and the observed heterozygosity, estimated on the basis of the genotypes of 7 individuals, ranged from 0...
December 19, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27999716/primary-cerebral-echinoccocosis-in-a-child-case-report-surgical-technique-technical-pitfalls-and-video-atlas
#20
Ahmed M A Altibi, Raed A H Qarajeh, Telmo A B Belsuzarri, Walid Maani, Tareq M A Kanaan
BACKGROUND: Hydatid disease is a life-threatening parasitic infestation caused by Echinococcus granulosus. Infection with E. granulosus typically results in the formation of hydatid cysts in the liver, lungs, kidney, and spleen. Primary intracranial hydatid cyst disease is extremely rare. Here, we are reporting an unusual case of Echinococcus, where the only identifiable lesion was a hydatid cyst in the brain without liver or lung involvement. We are also providing a description for the surgical technique used to remove the cyst, highlighting the possible surgical pitfalls...
2016: Surgical Neurology International
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