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Pathogen Inactivation

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https://www.readbyqxmd.com/read/29475122/blue-light-irradiation-triggers-the-antimicrobial-potential-of-zno-nanoparticles-on-drug-resistant-acinetobacter-baumannii
#1
Ming-Yeh Yang, Kai-Chih Chang, Liang-Yu Chen, Po-Ching Wang, Chih-Chiang Chou, Zhong-Bin Wu, Anren Hu
Photodynamic inactivation (PDI) is a non-invasive and safe therapeutic method for microbial infections. Bacterial antibiotic resistance is caused by antibiotics abuse. Drug-resistant Acinetobacter spp. is a serious problem in hospitals around the world. These pathogens from nosocomial infections have high mortality rates in frailer people, and Acinetobacter spp. is commonly found in immunocompromised patients. Visible light is safer than ultraviolet light (UV) for PDI of nosocomial pathogens with mammalian cells...
February 6, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29473173/ultraviolet-light-based-pathogen-inactivation-and-alloimmunization-after-platelet-transfusion-results-from-a-randomized-trial
#2
Philip J Norris, Zhanna Kaidarova, Erica Maiorana, Silvano Milani, Mila Lebedeva, Michael P Busch, Brian Custer, Paolo Rebulla
BACKGROUND: The current study explored whether pathogen-reduction treatment of platelet components before transfusion would decrease the risk of alloimmunization. STUDY DESIGN AND METHODS: Study participants were patients with hematologic cancer who were included in two parallel, randomized clinical trials testing pathogen-reduction treatment versus conventional platelets using the Mirasol or Intercept pathogen-reduction systems. Patients who had a baseline, pretransfusion sample and a follow-up, posttransfusion sample were included in the study (n = 179 patients in each study arm)...
February 22, 2018: Transfusion
https://www.readbyqxmd.com/read/29472902/prevention-and-control-strategies-to-counter-zika-virus-a-special-focus-on-intervention-approaches-against-vector-mosquitoes-current-updates
#3
REVIEW
Raj K Singh, Kuldeep Dhama, Rekha Khandia, Ashok Munjal, Kumaragurubaran Karthik, Ruchi Tiwari, Sandip Chakraborty, Yashpal S Malik, Rubén Bueno-Marí
Zika virus (ZIKV) is the most recent intruder that acquired the status of global threat creating panic and frightening situation to public owing to its rapid spread, attaining higher virulence and causing complex clinical manifestations including microcephaly in newborns and Guillain Barré Syndrome. Alike other flaviviruses, the principal mode of ZIKV transmission is by mosquitoes. Advances in research have provided reliable diagnostics for detecting ZIKV infection, while several drug/therapeutic targets and vaccine candidates have been identified recently...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29472641/bigr-is-a-sulfide-sensor-that-regulates-a-sulfur-transferase-dioxygenase-required-for-aerobic-respiration-of-plant-bacteria-under-sulfide-stress
#4
Nayara Patricia Vieira de Lira, Bianca Alves Pauletti, Ana Carolina Marques, Carlos Alberto Perez, Raquel Caserta, Alessandra Alves de Souza, Aníbal Eugênio Vercesi, Adriana Franco Paes Leme, Celso Eduardo Benedetti
To cope with toxic levels of H2 S, the plant pathogens Xylella fastidiosa and Agrobacterium tumefaciens employ the bigR operon to oxidize H2 S into sulfite. The bigR operon is regulated by the transcriptional repressor BigR and it encodes a bifunctional sulfur transferase (ST) and sulfur dioxygenase (SDO) enzyme, Blh, required for H2 S oxidation and bacterial growth under hypoxia. However, how Blh operates to enhance bacterial survival under hypoxia and how BigR is deactivated to derepress operon transcription is unknown...
February 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29471092/purification-and-functional-characterization-of-recombinant-balsamin-a-ribosome-inactivating-protein-from-momordica-balsamina
#5
Parminder K Ajji, Shailendra P Sonkar, Ken Walder, Munish Puri
Balsamin, a type I ribosome-inactivating protein (RIP), has been shown to inhibit HIV-1 replication at the translation step. Our recent studies have shown that balsamin also possess anti-tumor, antibacterial and DNase-like activity, however, the amount of natural balsamin in Momordica balsamina seeds is limited and preclinical studies require large quantities of pure, bioactive balsamin. Therefore, in this study, we cloned the balsamin gene, expressed it in E. coli BL21(DE3) strain and purified it by nickel affinity chromatography...
February 19, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29470411/stroke-like-episodes-and-cerebellar-syndrome-in-phosphomannomutase-deficiency-pmm2-cdg-evidence-for-hypoglycosylation-driven-channelopathy
#6
Mercè Izquierdo-Serra, Antonio F Martínez-Monseny, Laura López, Julia Carrillo-García, Albert Edo, Juan Darío Ortigoza-Escobar, Óscar García, Ramón Cancho-Candela, M Llanos Carrasco-Marina, Luis G Gutiérrez-Solana, Daniel Cuadras, Jordi Muchart, Raquel Montero, Rafael Artuch, Celia Pérez-Cerdá, Belén Pérez, Belén Pérez-Dueñas, Alfons Macaya, José M Fernández-Fernández, Mercedes Serrano
Stroke-like episodes (SLE) occur in phosphomannomutase deficiency (PMM2-CDG), and may complicate the course of channelopathies related to Familial Hemiplegic Migraine (FHM) caused by mutations in CACNA1A (encoding CaV 2.1 channel). The underlying pathomechanisms are unknown. We analyze clinical variables to detect risk factors for SLE in a series of 43 PMM2-CDG patients. We explore the hypothesis of abnormal CaV 2.1 function due to aberrant N -glycosylation as a potential novel pathomechanism of SLE and ataxia in PMM2-CDG by using whole-cell patch-clamp, N -glycosylation blockade and mutagenesis...
February 22, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29467765/pathogenic-bacterium-acinetobacter-baumannii-inhibits-the-formation-of-neutrophil-extracellular-traps-by-suppressing-neutrophil-adhesion
#7
Go Kamoshida, Takane Kikuchi-Ueda, Satoshi Nishida, Shigeru Tansho-Nagakawa, Tsuneyuki Ubagai, Yasuo Ono
Hospital-acquired infections caused by Acinetobacter baumannii have become problematic because of high rates of drug resistance. A. baumannii is usually harmless, but it may cause infectious diseases in an immunocompromised host. Although neutrophils are the key players of the initial immune response against bacterial infection, their interactions with A. baumannii remain largely unknown. A new biological defense mechanism, termed neutrophil extracellular traps (NETs), has been attracting attention. NETs play a critical role in bacterial killing by bacterial trapping and inactivation...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29463615/thioester-containing-proteins-2-and-4-affect-the-metabolic-activity-and-inflammation-response-in-drosophila
#8
Upasana Shokal, Hannah Kopydlowski, Sneh Harsh, Ioannis Eleftherianos
Drosophila melanogaster is an outstanding model for studying host anti-pathogen defense. Although substantial progress has been made in understanding how metabolism and immunity are interrelated in flies, little information has been obtained on the molecular players that regulate metabolism and inflammation in Drosophila during pathogenic infection. Recently we have reported that inactivation of Thioester containing proteins 2 and 4 ( Tep2 and Tep4 ) promotes survival and decreases the bacterial burden in flies upon infection with the virulent pathogens Photorhabdus luminescens and P...
February 20, 2018: Infection and Immunity
https://www.readbyqxmd.com/read/29463414/natural-meroterpenoids-isolated-from-the-plant-pathogenic-fungus-verticillium-albo-atrum-with-noteworthy-modification-action-against-voltage-gated-sodium-channels-of-central-neurons-of-helicoverpa-armigera
#9
Guangyan Wu, Lin Li, Bo Chen, Chuan Chen, Duqiang Luo, Bingjun He
A new meroterpenoid, named acetoxydehydroaustin A (1) and the known meroterpenoid austin (2) were isolated from the plant pathogenic fungus Verticillium albo-atrum. Their structures were established based on general spectroscopic techniques and the relative configuration of compound 1 was determined by single-crystal X-ray diffraction analysis. We first investigated and identified their significant electrophysiological effects on the gating kinetics of voltage-gated sodium channels in central neurons acutely dissociated from Helicoverpa armigera using whole-cell patch clamp technique...
January 2018: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/29463238/characterization-and-heterologous-expression-of-the-neoabyssomicin-abyssomicin-biosynthetic-gene-cluster-from-streptomyces-koyangensis-scsio-5802
#10
Jiajia Tu, Siting Li, Jiang Chen, Yongxiang Song, Shaobin Fu, Jianhua Ju, Qinglian Li
BACKGROUND: The deep-sea-derived microbe Streptomyces koyangensis SCSIO 5802 produces neoabyssomicins A-B (1-2) and abyssomicins 2 (3) and 4 (4). Neoabyssomicin A (1) augments human immunodeficiency virus-1 (HIV-1) replication whereas abyssomicin 2 (3) selectively reactivates latent HIV and is also active against Gram-positive pathogens including methicillin-resistant Staphylococcus aureus (MRSA). Structurally, neoabyssomicins A-B constitute a new subtype within the abyssomicin family and feature unique structural traits characteristic of extremely interesting biosynthetic transformations...
February 20, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29459630/development-of-ultra-deep-targeted-rna-sequencing-for-analyzing-x-chromosome-inactivation-in-female-dent-disease
#11
Shogo Minamikawa, Kandai Nozu, Yoshimi Nozu, Tomohiko Yamamura, Mariko Taniguchi-Ikeda, Keita Nakanishi, Junya Fujimura, Tomoko Horinouchi, Yuko Shima, Koichi Nakanishi, Masuji Hattori, Kyoko Kanda, Ryojiro Tanaka, Naoya Morisada, China Nagano, Nana Sakakibara, Hiroaki Nagase, Ichiro Morioka, Hiroshi Kaito, Kazumoto Iijima
The pattern of X-chromosome inactivation (XCI) can affect the clinical severity of X-linked disorders in females. XCI pattern analysis has been conducted mainly by HUMARA assay, a polymerase chain reaction-based assay using a methylation-sensitive restriction enzyme. However, this assay examines the XCI ratio of the androgen receptor gene at the genomic DNA level and does not reflect the ratio of either targeted gene directly or at the mRNA level. Here, we report four females with Dent disease, and we clarified the correlation between XCI and female cases of Dent disease using not only HUMARA assay but also a novel analytical method by RNA sequencing...
February 19, 2018: Journal of Human Genetics
https://www.readbyqxmd.com/read/29459176/variations-of-t-and-b-lymphocytes-of-flounder-paralichthys-olivaceus-after-hirame-novirhabdovirus-infection-and-immunization
#12
Jing Xing, Lei Wang, Mengxiao Zhen, Xiaoqian Tang, Wenbin Zhan
T and B lymphocytes are closely related to immunization and pathogen infection. Our previous study confirmed the CD3+ , CD4-1+ , CD4-2+ , CD8β+ T lymphocytes and IgM+ B lymphocytes presented in the peripheral blood leukocytes (PBLs) of flounder (Paralichthys olivaceus), in this paper, the variations of T and B lymphocytes of flounder after Hirame novirhabdovirus (HIRRV) infection or immunization were investigated. The flounders were injected with live or inactivated HIRRV, then the percentages of T and B lymphocytes in PBLs were analyzed by Flow cytometry (FCM), total antibodies and HIRRV-specific antibodies in serum were detected by Enzyme-linked immunosorbent assay (ELISA), and expression of twelve immune-related genes in the head kidneys were determined using q-PCR...
February 16, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29458687/maldi-tof-mass-spectrometry-and-high-consequence-bacteria-safety-and-stability-of-biothreat-bacterial-sample-testing-in-clinical-diagnostic-laboratories
#13
Dobryan M Tracz, Ashley D Tober, Kym S Antonation, Cindi R Corbett
We considered the application of MALDI-TOF mass spectrometry for BSL-3 bacterial diagnostics, with a focus on the biosafety of live-culture direct-colony testing and the stability of stored extracts. Biosafety level 2 (BSL-2) bacterial species were used as surrogates for BSL-3 high-consequence pathogens in all live-culture MALDI-TOF experiments. Viable BSL-2 bacteria were isolated from MALDI-TOF mass spectrometry target plates after 'direct-colony' and 'on-plate' extraction testing, suggesting that the matrix chemicals alone cannot be considered sufficient to inactivate bacterial culture and spores in all samples...
February 2, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29457249/gut-bacteria-of-cockroaches-are-a-potential-source-of-antibacterial-compound-s
#14
Noor Akbar, Ruqaiyyah Siddiqui, Mazhar Iqbal, K Sagathevan, Naveed Ahmed Khan
Here, we hypothesized that the microbial gut flora of animals/pests living in polluted environments, produce substances to thwart bacterial infections. The overall aim of this study was to source microbes inhabiting unusual environmental niches for potential antimicrobial activity. Two cockroach species, Gromphadorhina portentosa (Madagascar) and Blaptica dubia (Dubia) were selected. The gut bacteria from these species were isolated and grown in RPMI 1640 and conditioned media were prepared. Conditioned media were tested against a panel of Gram-positive (Methicillin resistant Staphylococcus aureus, Streptococcus pyogenes, Bacillus cereus) and Gram-negative (Escherichia coli K1, Salmonella enterica, Serratia marcescens, Pseudomonas aeruginosa, Klebsiella pneumoniae) bacteria, as well as the protist pathogen, Acanthamoeba castellanii...
February 19, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29453966/gamma-irradiation-inactivates-honey-bee-fungal-microsporidian-and-viral-pathogens-and-parasites
#15
Michael Simone-Finstrom, Kate Aronstein, Michael Goblirsch, Frank Rinkevich, Lilia de Guzman
Managed honey bee (Apis mellifera) populations are currently facing unsustainable losses due to a variety of factors. Colonies are challenged with brood pathogens, such as the fungal agent of chalkbrood disease, the microsporidian gut parasite Nosema sp., and several viruses. These pathogens may be transmitted horizontally from worker to worker, vertically from queen to egg and via vectors like the parasitic mite, Varroa destructor. Despite the fact that these pathogens are widespread and often harbored in wax comb that is reused from year to year and transferred across beekeeping operations, few, if any, universal treatments exist for their control...
February 14, 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29452196/staphylococcus-aureus-induces-tgf-%C3%AE-1-and-bfgf-expression-through-the-activation-of-ap-1-and-nf-%C3%AE%C2%BAb-transcription-factors-in-bovine-mammary-epithelial-cells
#16
Jianmei Wu, Yulin Ding, Jinling Wang, Fenglong Wang
Staphylococcus aureus is a common Gram-positive pathogen that causes bovine mastitis, a persistent infection of the bovine mammary gland. Bovine mammary epithelial cells (BMEC) are important parenchymal cells of the bovine mammary gland. To better understand the importance of BMEC and the roles of the TLR-NF-κBand TLR-AP-1 signaling pathways in the regulation of S. aureus-associated mastitis and mammary fibrosis, BMEC cultured in vitro were stimulated with different concentrations of heat-inactivated S. aureus to analyze the gene and protein expression and production of toll-like receptor 2 (TLR2), toll-like receptor 4 (TLR4), transforming growth factor beta 1 (TGF-β1), basic fibroblast growth factor (bFGF) as well as the protein expression of nuclear factor-kappa B (NF-κB) and activation protein-1 (AP-1) by means of quantitative polymerase chain reaction (qPCR) and western blotting, respectively...
February 13, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29449721/bi-allelic-inactivating-variants-in-the-coch-gene-cause-autosomal-recessive-prelingual-hearing-impairment
#17
Sebastien P F JanssensdeVarebeke, Guy Van Camp, Nils Peeters, Ellen Elinck, Josine Widdershoven, Tony Cox, Kristof Deben, Katrien Ketelslagers, Tom Crins, Wim Wuyts
Pathogenic variant in COCH are a known cause of DFNA9 autosomal dominant progressive hearing loss and vestibular dysfunction with adult onset. Hitherto, only dominant nonsynonymous variants and in-frame deletions with a presumed dominant negative or gain-of-function effect have been described. Here, we describe two brothers with congenital prelingual deafness and a homozygous nonsense c.292C>T(p.Arg98*) COCH variant, suggesting a loss-of-function effect. Vestibular dysfunction starting in the first decade was observed in the older patient...
February 15, 2018: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/29449720/biallelic-inactivating-variants-in-the-gtpbp2-gene-cause-a-neurodevelopmental-disorder-with-severe-intellectual-disability
#18
Aida M Bertoli-Avella, Jose M Garcia-Aznar, Oliver Brandau, Fahad Al-Hakami, Zafer Yüksel, Anett Marais, Nana-Maria Grüning, Lia Abbasi Moheb, Omid Paknia, Nahla Alshaikh, Seham Alameer, Makia J Marafi, Fahd Al-Mulla, Nouriya Al-Sannaa, Arndt Rolfs, Peter Bauer
Congenital neurological disorders are genetically highly heterogeneous. Rare forms of hereditary neurological disorders are still difficult to be adequately diagnosed. Pertinent studies, especially when reporting only single families, need independent confirmation. We present three unrelated families in which whole-exome sequencing identified the homozygous non-sense variants c.430[C>T];[C>T] p.(Arg144*), c.1219[C>T];[C>T] p.(Gln407*) and c.1408[C>T];[C>T] p.(Arg470*) in GTPBP2. Their clinical presentations include early onset and apparently non-progressive motor and cognitive impairment, and thereby overlap with findings in a recently described family harbouring a homozygous GTPBP2 splice site variant...
February 15, 2018: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/29447282/addition-of-m6a-to-sv40-late-mrnas-enhances-viral-structural-gene-expression-and-replication
#19
Kevin Tsai, David G Courtney, Bryan R Cullen
Polyomaviruses are a family of small DNA tumor viruses that includes several pathogenic human members, including Merkel cell polyomavirus, BK virus and JC virus. As is characteristic of DNA tumor viruses, gene expression in polyomaviruses is temporally regulated into an early phase, consisting of the viral regulatory proteins, and a late phase, consisting of the viral structural proteins. Previously, the late transcripts expressed by the prototypic polyomavirus simian virus 40 (SV40) were reported to contain several adenosines bearing methyl groups at the N6 position (m6A), although the precise location of these m6A residues, and their phenotypic effects, have not been investigated...
February 15, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29441601/refrigeration-cryopreservation-and-pathogen-inactivation-an-updated-perspective-on-platelet-storage-conditions
#20
REVIEW
L Waters, M Cameron, M P Padula, D C Marks, L Johnson
Conventional storage of platelet concentrates limits their shelf life to between 5 and 7 days due to the risk of bacterial proliferation and the development of the platelet storage lesion. Cold storage and cryopreservation of platelets may facilitate extension of the shelf life to weeks and years, and may also provide the benefit of being more haemostatically effective than conventionally stored platelets. Further, treatment of platelet concentrates with pathogen inactivation systems reduces bacterial contamination and provides a safeguard against the risk of emerging and re-emerging pathogens...
February 14, 2018: Vox Sanguinis
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