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https://www.readbyqxmd.com/read/28214745/rapid-detection-and-quantification-of-enterovirus-71-by-a-portable-surface-plasmon-resonance-biosensor
#1
Briliant Adhi Prabowo, Robert Y L Wang, Muhammad Khari Secario, Po-Ting Ou, Azharul Alom, Jia-Jung Liu, Kou-Chen Liu
This study presents the first report on a label-free detection and rapid quantification method for human enterovirus 71 (EV71) using a portable surface plasmon resonance (SPR) system. The SPR sensor instrument was configured to run on low power in a miniaturized platform to improve the device portability for a wider application both in laboratories and in the field. A color tunable organic light emitting diode in red spectrum was attached on a trapezoidal prism for the disposable light source module. The SPR signal processing using integration area under the reflectivity curve is applied for optimum signal to noise ratio (SNR) enhancement...
January 21, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28214674/development-of-a-quick-serum-il-6-measuring-system-in-rheumatoid-arthritis
#2
Kensuke Koyama, Tetsuro Ohba, Kentaro Ishii, Giman Jung, Hirotaka Haro, Kenichi Matsuda
OBJECTIVE: Interleukin-6 (IL-6) plays a crucial role in the pathogenesis of rheumatoid arthritis (RA). Both fulfillment of remission criteria and assessment of other methods of evaluation of RA are important for preventing joint damage progression. Measurement of serum IL-6 concentrations has been reported to be useful for monitoring RA disease activity. However, it takes at least 4-5h to measure serum IL-6 concentrations using traditional methods, which limits its utility during routine assessment in daily clinical practice settings...
February 16, 2017: Cytokine
https://www.readbyqxmd.com/read/28214402/relationship-between-chewing-ability-and-cognitive-impairment-in-the-rural-elderly
#3
Eun-Kyong Kim, Sung Kook Lee, Youn-Hee Choi, Makiko Tanaka, Kimiko Hirotsu, Hyeon Chang Kim, Hee-Kyung Lee, Yun-Sook Jung, Atsuo Amano
OBJECTIVE: Relationship between masticatory function and cognitive impairment had been suggested but still understudied. We investigated the association between chewing ability and cognitive impairment among the elderly living in a rural region. METHODS: A total of 295 elderly individuals aged ≥70 years in a rural city of Korea participated in a cross-sectional study. Trained nurses conducted interviews and assessed chewing ability using gum that changed color based on chewing performance...
February 11, 2017: Archives of Gerontology and Geriatrics
https://www.readbyqxmd.com/read/28214208/reduced-immunohistochemical-expression-of-adhesion-molecules-in-vitiligo-skin-biopsies
#4
Adriane Reichert Faria, Juliana Elizabeth Jung, Caio César Silva de Castro, Lucia de Noronha
Because defects in adhesion impairment seem to be involved in the etiopathogenesis of vitiligo, this study aimed to compare the immunohistochemical expression of several adhesion molecules in the epidermis of vitiligo and non lesional vitiligo skin. Sixty-six specimens of lesional and non lesional skin from 33 volunteers with vitiligo were evaluated by immunohistochemistry using anti-beta-catenin, anti-E-cadherin, anti-laminin, anti-beta1 integrin, anti-collagen IV, anti-ICAM-1 and anti-VCAM-1 antibodies. Biopsies of vitiligo skin demonstrated a significant reduction in the expression of laminin and integrin...
December 30, 2016: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28214200/concurrent-met-copy-number-gain-and-kras-mutation-is-a-poor-prognostic-factor-in-pancreatobiliary-subtype-ampullary-cancers
#5
Mi Jung Kwon, Jeong Won Kim, Jang Yong Jeon, Eun Sook Nam, Seong Jin Cho, Hye-Rim Park, Soo Kee Min, Jinwon Seo, Kyueng-Whan Min, Ji-Young Choe, Hye Kyung Lee
Hepatocyte growth factor (HGF) and MET are candidates of targeted therapies for cancer patients. Although MET and HGF are commonly expressed in biliary tract cancers, their expression and gene copy number status and their association with KRAS mutations have not been investigated in pancreatobiliary-type ampullary adenocarcinomas (A-ACs), one of the aggressive periampullary cancers. MET and HGF expressions and MET copy number status were examined by performing immunohistochemistry (IHC) and silver in situ hybridization (SISH) in 62 surgically resected, paraffin-embedded tumors, respectively...
January 11, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28213903/specifics-of-fetuin-a-levels-in-distinct-types-of-chronic-heart-failure
#6
Michael Lichtenauer, Bernhard Wernly, Vera Paar, Ilonka Rohm, Christian Jung, Atilla Yilmaz, Uta C Hoppe, Paul Christian Schulze, Daniel Kretzschmar, Rudin Pistulli
INTRODUCTION: Fetuin-A has been described to correlate inversely with vascular calcification both in animal models but also in patients with heart and renal disease. In this current study, we sought to investigate whether fetuin-A might be a useful marker for the discrimination of ischemic (ICM) from dilated cardiomyopathy (DCM). METHODS: A total of 124 non-consecutive patients were included in this study, 59 patients suffered from ICM and 65 patients from DCM. Serum samples were obtained during out-patient visits and analyzed for fetuin-A by ELISA...
February 18, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28213678/unifying-differential-item-functioning-in-factor-analysis-for-categorical-data-under-a-discretization-of-a-normal-variant
#7
Yu-Wei Chang, Nan-Jung Hsu, Rung-Ching Tsai
The multiple-group categorical factor analysis (FA) model and the graded response model (GRM) are commonly used to examine polytomous items for differential item functioning to detect possible measurement bias in educational testing. In this study, the multiple-group categorical factor analysis model (MC-FA) and multiple-group normal-ogive GRM models are unified under the common framework of discretization of a normal variant. We rigorously justify a set of identified parameters and determine possible identifiability constraints necessary to make the parameters just-identified and estimable in the common framework of MC-FA...
February 17, 2017: Psychometrika
https://www.readbyqxmd.com/read/28213522/type-i-interferon-regulated-gene-expression-and-signaling-in-murine-mixed-glial-cells-lacking-signal-transducers-and-activators-of-transcription-1-or-2-or-interferon-regulatory-factor-9
#8
Wen Li, Markus J Hofer, Pattama Songkhunawej, So Ri Jung, Dale Hancock, Gareth Denyer, Iain L Campbell
Type I interferons (IFN-I) are critical in antimicrobial and antitumor defense. Although IFN-I signal via the interferon-stimulated gene factor 3 (ISGF3) complex consisting of STAT1, STAT2 and IRF9, IFN-I can mediate significant biological effects via ISGF3-independent pathways. For example, absence of STAT1, STAT2 or IRF9 exacerbates neurological disease in transgenic mice with CNS-production of IFN-Ι. Here we determined the role of IFN-I-driven, ISGF3-independent signaling in regulating global gene expression in STAT1, STAT2 or IRF9-deficient murine mixed glial cell cultures (MGCs)...
February 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28213388/effects-of-dopamine-donor-pretreatment-on-graft-survival-after-kidney-transplantation-a-randomized-trial
#9
Peter Schnuelle, Wilhelm H Schmitt, Christel Weiss, Antje Habicht, Lutz Renders, Martin Zeier, Felix Drüschler, Katharina Heller, Przemyslaw Pisarski, Bernhard Banas, Bernhard K Krämer, Matthias Jung, Kai Lopau, Christoph J Olbricht, Horst Weihprecht, Peter Schenker, Johan W De Fijter, Benito A Yard, Urs Benck
BACKGROUND AND OBJECTIVES: Donor dopamine improves initial graft function after kidney transplantation due to antioxidant properties. We investigated if a 4 µg/kg per minute continuous dopamine infusion administered after brain-death confirmation affects long-term graft survival and examined the exposure-response relationship with treatment duration. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: Five-year follow-up of 487 renal transplant patients from 60 European centers who had participated in the randomized, multicenter trial of dopamine donor pretreatment between 2004 and 2007 (ClinicalTrials...
February 17, 2017: Clinical Journal of the American Society of Nephrology: CJASN
https://www.readbyqxmd.com/read/28213110/effect-of-chinese-herbal-medicine-on-stroke-patients-with-type-2-diabetes
#10
Fuu-Jen Tsai, Tsung-Jung Ho, Chi-Fung Cheng, Xiang Liu, Hsinyi Tsang, Ting-Hsu Lin, Chiu-Chu Liao, Shao-Mei Huang, Ju-Pi Li, Cheng-Wen Lin, Jaung-Geng Lin, Jung-Chun Lin, Chih-Chien Lin, Wen-Miin Liang, Ying-Ju Lin
ETHNOPHARMACOLOGICAL RELEVANCE: Complications of type 2 diabetes (T2D) include stroke, which is a cerebrovascular disturbance characterized by reduced blood flow in the brain, leading to death or physical disability. Chinese herbal medicine (CHM) has been widely used in ancient China for the treatment of diabetes and stroke by supplementing Qi and activating blood circulation. AIM OF THE STUDY: This study aimed to investigate the frequencies and patterns of CHM treatment for stroke patients with T2D and the outcomes of long-term use in Taiwan...
February 14, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28213043/emerging-insights-into-the-biology-of-typhoid-toxin
#11
REVIEW
Casey C Fowler, Shu-Jung Chang, Xiang Gao, Tobias Geiger, Gabrielle Stack, Jorge E Galán
Typhoid toxin is a unique A2B5 exotoxin and an important virulence factor for Salmonella Typhi, the cause of typhoid fever. In the decade since its initial discovery, great strides have been made in deciphering the unusual biological program of this toxin, which is fundamentally different from related toxins in many ways. Purified typhoid toxin administered to laboratory animals causes many of the symptoms of typhoid fever, suggesting that typhoid toxin is a central factor in this disease. Further advances in understanding the biology of this toxin will help guide the development of badly needed diagnostics and therapeutic interventions that target this toxin to detect, prevent or treat typhoid fever...
February 14, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/28213032/applicability-of-a-colorimetric-method-for-evaluation-of-lactic-acid-bacteria-with-probiotic-properties
#12
Min Young Jung, Jina Lee, Boyeon Park, Hyelyeon Hwang, Sung-Oh Sohn, Se Hee Lee, Hyeong In Lim, Hae Woong Park, Jong-Hee Lee
Rapid colorimetric methods using various indicator reagents have been developed to monitor bacterial viability. Here, we examined the applicability of a method based on the reduction of resazurin or water-soluble tetrazolium salt-8 (WST-8) to screen lactic acid bacteria (LAB) for growth, tolerance against bile acid and low pH. The resazurin reduction test proved unsuitable for screening LAB such as Lactobacillus plantarum and Leuconostoc mesenteroides since it reacted with acid present in the cultures. LAB growth could be indirectly quantified by measuring WST-8 reduction...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28213024/sanitizer-efficacy-in-preventing-cross-contamination-of-heads-of-lettuce-during-retail-crisping
#13
Yangjin Jung, Hyein Jang, Mengqi Guo, Jingwen Gao, Karl R Matthews
This study was conducted to provide information regarding mitigation of cross-contamination through the use of sanitizer during crisping at retail outlets. Seven non-inoculated heads and one inoculated head (≈5 log CFU/g) of lettuce were placed into commercial sink filled with 76 L of tap water (TW), electrolyzed water (EW, free chlorine: 43 ± 6 ppm), lactic acid and phosphoric acid-based sanitizer (LPA, pH 2.89), or citric acid-based sanitizer (CA, pH 2.78) and soaked for 5 min. Two subsequent batches (eight non-inoculated heads per batch) were soaked in the same solution...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28212991/different-histological-subtypes-of-peripheral-lung-cancer-based-on-emphysema-distribution-in-patients-with-both-airflow-limitation-and-ct-determined-emphysema
#14
Beomsu Shin, Sumin Shin, Myung Jin Chung, Hyun Lee, Won-Jung Koh, Hojoong Kim, Hye Yun Park
The histological subtypes by peripheral tumor location remain uncharacterized in COPD patients with emphysema. We investigated histologic subtypes of peripheral lung cancers based on the context of heterogeneous emphysema distribution in patients with airflow limitation and CT-determined emphysema. A retrospective, cross-sectional study was conducted using data from 754 patients with airflow limitation and newly-diagnosed primary lung cancers from February 2013 to February 2015. Of these, 230 patients had emphysema, as determined by computed tomography software designed to quantify emphysema...
February 2017: Lung Cancer: Journal of the International Association for the Study of Lung Cancer
https://www.readbyqxmd.com/read/28212773/a-grayscale-photograph-with-high-dynamic-range-taken-under-a-wood-s-lamp-for-better-recognition-of-vitiligo-lesions
#15
Jung Min Bae, Tae Young Han
No abstract text is available yet for this article.
March 2017: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/28212771/a-girl-with-a-solitary-red-bulla
#16
Eun Jung Byun, Sang Hyun Cho, Jeong Deuk Lee, Hei Sung Kim
No abstract text is available yet for this article.
March 2017: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/28212632/exome-wide-association-study-identifies-genetic-polymorphisms-of-c12orf51-myl2-and-aldh2-associated-with-blood-lead-levels-in-the-general-korean-population
#17
Sang-Yong Eom, Myung Sil Hwang, Ji-Ae Lim, Byung-Sun Choi, Ho-Jang Kwon, Jung-Duck Park, Yong-Dae Kim, Heon Kim
BACKGROUND: Lead (Pb) is a ubiquitous toxic metal present in the environment that poses adverse health effects to humans. Inter-individual variation in blood Pb levels is affected by various factors, including genetic makeup. However, limited data are available on the association between genetic variation and blood Pb levels. The purpose of this study was to identify the genetic markers associated with blood Pb levels in the Korean population. METHODS: The study subjects consisted of 1,483 healthy adults with no history of occupational exposure to Pb...
February 17, 2017: Environmental Health: a Global Access Science Source
https://www.readbyqxmd.com/read/28212594/percutaneous-full-endoscopic-treatment-of-bertolotti-syndrome-a-report-of-three-cases-with-technical-note
#18
Byapak Paudel, Hyeun-Sung Kim, Jee Soo Jang, Jeong Hoon Choi, Sung Kyun Chung, Jung Sup Lee, Jeong Hoon Kim, Seong Hoon Oh, Il Tae Jang
Background Bertolotti syndrome is characterized by an abnormal enlargement of the transverse process of the most caudal lumbar vertebra. Most of the time it is asymptomatic, but when it is symptomatic it is associated with low back pain, radiating leg pain, or both. There is no consensus regarding management of this pathology. Open to minimal invasive tubular resection techniques are described in the literature, but a full endoscopic resection technique has not yet been described. Endoscopic technique is a less invasive target-oriented iliolumbar ligament preserving technique...
February 17, 2017: Journal of Neurological Surgery. Part A, Central European Neurosurgery
https://www.readbyqxmd.com/read/28212583/role-of-topk-in-lipopolysaccharide-induced-breast-cancer-cell-migration-and-invasion
#19
Min-Ah Seol, Jung-Hwan Park, Ji Heun Jeong, Jungmook Lyu, Seung Yun Han, Sang-Muk Oh
Inflammation has been known to be linked to invasion or metastasis of breast cancer, which has poor prognosis, although the regulatory mechanism remains to be undiscovered. Here we show that T-LAK cell-originated protein kinase (TOPK) mediates pro-inflammatory endotoxin lipopolysaccharide (LPS)-induced breast cancer cell migration and invasion. The mRNA or protein level of TOPK, toll- like receptor4 (TLR4), interleukin (IL)-6, vascular endothelial growth factor (VEGF) or matrix metalloproteinase9 (MMP9) genes related to TLR4 signaling or tumor progression was induced by LPS treatment in MCF7 breast cancer cells, but the induction was abolished by stable knocking down of TOPK in MCF7 cells...
February 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28212571/activation-of-jnk-and-ire1-is-critically-involved-in-tanshinone-i-induced-p62-dependent-autophagy-in-malignant-pleural-mesothelioma-cells-implication-of-p62-uba-domain
#20
Jihyun Lee, Eun Jung Sohn, Sangwook Yoon, Gunho Won, Chang Geun Kim, Ji Hoon Jung, Sung-Hoon Kim
The aim of present study is to elucidate autophagic mechanism of tanshinone I (Tan I) in H28 and H2452 mesothelioma cells. Herein, Tan I exerted cytotoxicity with autophagic features of autophagy protein 5 (ATG5)/ microtubule-associated protein 1A/1B-light chain 3II (LC3 II) activation, p62/sequestosome 1 (SQSTM1) accumulation and increased number of LC3II punctae, acridine orange-stained cells and autophagic vacuoles. However, 3-methyladenine (3MA) and NH4Cl increased cytotoxicity in Tan I treated H28 cells...
February 15, 2017: Oncotarget
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