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Dengue NS1

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https://www.readbyqxmd.com/read/28791003/dengue-virus-glycosylation-what-do-we-know
#1
REVIEW
Sally S L Yap, Terry Nguyen-Khuong, Pauline M Rudd, Sylvie Alonso
In many infectious diseases caused by either viruses or bacteria, pathogen glycoproteins play important roles during the infection cycle, ranging from entry to successful intracellular replication and host immune evasion. Dengue is no exception. Dengue virus glycoproteins, envelope protein (E) and non-structural protein 1 (NS1) are two popular sub-unit vaccine candidates. E protein on the virion surface is the major target of neutralizing antibodies. NS1 which is secreted during DENV infection has been shown to induce a variety of host responses through its binding to several host factors...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28770247/age-of-initial-cohort-of-dengue-patients-could-explain-the-origin-of-disease-outbreak-in-a-setting-a-case-control-study-in-rajasthan-india
#2
Annette Angel, Bennet Angel, Karuna Yadav, Neha Sharma, Vinod Joshi, Indu Thanvi, Sharad Thanvi
Dengue fever (DF) and dengue hemorrhagic fever (DHF) is a public health problem with 390 million cases reported in world annually. In Rajasthan, DF with DHF is being reported for about two decades. For undertaking interventions into disease transmission, locating origin of transmission is very important. Present paper reports retrospective analysis of the hospital reported cases of dengue during the year 2013-2014 undertaken in Barmer, Rajasthan. To address task of investigating outbreak, detailed analysis of the data on serological test results (Mac-ELISA assay of NS1, IgG and IgM) performed by local hospital, Balotra was made...
June 2017: Virusdisease
https://www.readbyqxmd.com/read/28765561/antibodies-against-modified-ns1-wing-domain-peptide-protect-against-dengue-virus-infection
#3
Yen-Chung Lai, Yung-Chun Chuang, Ching-Chuan Liu, Tzong-Shiann Ho, Yee-Shin Lin, Robert Anderson, Trai-Ming Yeh
Dengue is the most common mosquito-transmitted viral infection for which an improved vaccine is still needed. Although nonstructural protein-1 (NS1) immunization can protect mice against dengue infection, molecular mimicry between NS1 and host proteins makes NS1-based vaccines challenging to develop. Based on the epitope recognized by the anti-NS1 monoclonal Ab (mAb) 33D2 which recognizes a conserved NS1 wing domain (NS1-WD) region but not host proteins, we synthesized a modified NS1-WD peptide to immunize mice...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28758625/the-dengue-virus-non-structural-protein-1-ns1-is-secreted-from-infected-mosquito-cells-via-a-non-classical-caveolin-1-dependent-pathway
#4
Ana C Alcalá, Raiza Hernández-Bravo, Fernando Medina, David S Coll, Jose L Zambrano, Rosa M Del Angel, Juan E Ludert
Dengue virus NS1 is a glycoprotein of 46-50 kDa that is conserved among flaviviruses, associates as a dimer to cell membranes and is secreted as a hexamer to the extracellular milieu. Recent evidence showed that NS1 is secreted efficiently from infected mosquito cells. To explore the secretory route of NS1 in mosquito cells, infected cells were treated with brefeldin A (BFA) and methyl-beta-cyclodextrin (MβCD). The results showed that MβCD, but not BFA, significantly reduced the release of NS1. Moreover, silencing the expression of caveolin-1 (CAV1; a key component of the caveolar system that transports cholesterol inside the cell), but not SAR1 (a GTPase that participates in the classical secretory pathway), also results in a significant reduction of the secretion of NS1...
July 31, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28756561/evidence-of-dengue-and-chikungunya-virus-co-infection-and-circulation-of-multiple-dengue-serotypes-in-a-recent-indian-outbreak
#5
S Mukherjee, S K Dutta, S Sengupta, A Tripathi
In India, dengue endemic areas overlap with chikungunya-affected areas and both the viruses are transmitted by same vector, Aedes aegypti - thereby increasing opportunity of co-infection by both viruses. Present study was carried out to understand the DENV-CHIKV infection dynamics during recent outbreaks in eastern India (West Bengal state) and its implication on disease manifestations. Blood was collected from 326 symptomatic febrile patients. Patients' serum was subjected to serological diagnosis for presence of anti-dengue-IgM, anti-chikungunya-IgM antibodies and dengue-NS1 antigen by ELISA...
July 29, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28749765/generation-and-characterization-of-antinonstructural-protein-1-monoclonal-antibodies-and-development-of-diagnostics-for-dengue-virus-serotype-2
#6
Yin-Liang Tang, I-Ju Liu, Pi-Chun Li, Chien-Yu Chiu, Chun-Yu Lin, Chung-Hao Huang, Yen-Hsu Chen, Chi-Yu Fu, Day-Yu Chao, Chwan-Chuen King, Han-Chung Wu
Dengue virus (DENV) circulates in tropical and subtropical areas around the world, where it causes high morbidity and mortality. There is no effective treatment of infection, with supportive care being the only option. Furthermore, early detection and diagnosis are important to facilitate clinical decisions. In this study, seven monoclonal antibodies (mAbs) recognizing nonstructural protein 1 (NS1) of DENV were generated by hybridoma techniques. These antibodies can be divided into two groups: serotype-specific (DB6-1, DB12-3, and DB38-1) and nonspecific (consisting of antibodies DB16-1, DB20-6, DB29-1, and DB41-2)...
July 24, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28744070/mri-features-in-dengue-encephalitis-a-case-series-in-south-indian-tertiary-care-hospital
#7
Brijesh K Soni, Debasheesh S R Das, R A George, R Aggarwal, R Sivasankar
Dengue virus, a RNA virus of family Flaviviradae is considered non-neurotropic. Increasing studies and case reports reveal neurological manifestations of dengue virus. In our case series, we have evaluated magnetic resonance imaging (MRI) findings of 3 patients with dengue fever diagnosed by positive dengue NS1 antigen with neurological symptoms, which revealed nonspecific imaging features of dengue encephalitis in two cases and dengue meningoencephalitis in one case. Autopsy findings are also correlated in 2 patients who succumbed to their disease...
April 2017: Indian Journal of Radiology & Imaging
https://www.readbyqxmd.com/read/28740517/dengue-virus-ns1-protein-as-a-diagnostic-marker-commercially-available-elisa-and-comparison-to-qrt-pcr-and-serological-diagnostic-assays-currently-used-by-the-state-of-florida
#8
Jason H Ambrose, Shamala Devi Sekaran, Azliyati Azizan
BACKGROUND: The proper management of patients infected with dengue virus requires early detection. Here, real-time molecular assays have proven useful but have limitations, whereas ELISAs that detect antibodies are still favored but results are obtained too late to be of clinical value. The production of DENV NS1 peaks early during infection and its detection can combine the advantages of both diagnostic approaches. METHODS: This study compared assays currently used for detecting DENV infection at the Florida Department of Health including anti-DENV IgM and IgG ELISAs as well as qRT-PCR, against a commercially available DENV NS1 ELISA...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28726595/co-circulation-and-co-infections-of-all-dengue-virus-serotypes-in-hyderabad-india-2014
#9
K Vaddadi, C Gandikota, P K Jain, V S V Prasad, M Venkataramana
The burden of dengue virus infections increased globally during recent years. Though India is considered as dengue hyper-endemic country, limited data are available on disease epidemiology. The present study includes molecular characterization of dengue virus strains occurred in Hyderabad, India, during the year 2014. A total of 120 febrile cases were recruited for this study, which includes only children and 41 were serologically confirmed for dengue positive infections using non-structural (NS1) and/or IgG/IgM ELISA tests...
July 20, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28720733/dengue-virus-hijacks-a-noncanonical-oxidoreductase-function-of-a-cellular-oligosaccharyltransferase-complex
#10
David L Lin, Natalia A Cherepanova, Leonia Bozzacco, Margaret R MacDonald, Reid Gilmore, Andrew W Tai
Dengue virus (DENV) is the most common arboviral infection globally, infecting an estimated 390 million people each year. We employed a genome-wide clustered regularly interspaced short palindromic repeat (CRISPR) screen to identify host dependency factors required for DENV propagation and identified the oligosaccharyltransferase (OST) complex as an essential host factor for DENV infection. Mammalian cells express two OSTs containing either STT3A or STT3B. We found that the canonical catalytic function of the OSTs as oligosaccharyltransferases is not necessary for DENV infection, as cells expressing catalytically inactive STT3A or STT3B are able to support DENV propagation...
July 18, 2017: MBio
https://www.readbyqxmd.com/read/28716913/antibody-based-assay-discriminates-zika-virus-infection-from-other-flaviviruses
#11
Angel Balmaseda, Karin Stettler, Raquel Medialdea-Carrera, Damaris Collado, Xia Jin, José Victor Zambrana, Stefano Jaconi, Elisabetta Cameroni, Saira Saborio, Francesca Rovida, Elena Percivalle, Samreen Ijaz, Steve Dicks, Ines Ushiro-Lumb, Luisa Barzon, Patricia Siqueira, David W G Brown, Fausto Baldanti, Richard Tedder, Maria Zambon, A M Bispo de Filippis, Eva Harris, Davide Corti
Zika virus (ZIKV) is a mosquito-borne flavivirus that emerged recently as a global health threat, causing a pandemic in the Americas. ZIKV infection mostly causes mild disease, but is linked to devastating congenital birth defects and Guillain-Barré syndrome in adults. The high level of cross-reactivity among flaviviruses and their cocirculation has complicated serological approaches to differentially detect ZIKV and dengue virus (DENV) infections, accentuating the urgent need for a specific and sensitive serological test...
August 1, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28716893/which-dengue-vaccine-approach-is-the-most-promising-and-should-we-be-concerned-about-enhanced-disease-after-vaccination-there-is-only-one-true-winner
#12
Scott B Halstead
The scientific community now possesses information obtained directly from human beings that makes it possible to understand why breakthrough-enhanced dengue virus (DENV) infections occurred in children receiving Sanofi Pasteur's Dengvaxia tetravalent live attenuated vaccine and to predict the possibility of breakthrough-enhanced DENV infections following immunization with two other tetravalent live attenuated vaccines now in phase III testing. Based upon recent research, Dengvaxia, lacking DENV nonstructural protein antigens, did not protect seronegatives because it failed to raise a competent T-cell response and/or antibodies to NS1...
July 17, 2017: Cold Spring Harbor Perspectives in Biology
https://www.readbyqxmd.com/read/28712817/clinical-features-and-dynamic-ordinary-laboratory-tests-differentiating-dengue-fever-from-other-febrile-illnesses-in-children
#13
Chih-Ho Chen, Yi-Chuan Huang, Kuang-Che Kuo, Chung-Chen Li
BACKGROUND: Dengue fever is not easily to be diagnosed before presentation of the classic symptoms. The study aimed to investigate the clinical features and dynamic laboratory tests in pediatric patients to facilitate dengue diagnosis. METHODS: This retrospective study examined the medical records of all pediatric patients who were clinically suspected to have dengue from June to December 2014. Laboratory-positive dengue cases were confirmed by detecting non-structural protein NS1, reverse transcription-polymerase chain reaction of dengue virus, and dengue-specific IgM seroconversion...
June 30, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28712153/proteomic%C3%A2-analysis-reveals-the-enhancement-of-human-serum-apolipoprotein-a-1-apo-a-1-in%C3%A2-individuals%C3%A2-infected-with-multiple-dengue-virus-serotypes
#14
Manchala Nageswar Reddy, Ranjeet Dungdung, Rajendra Pilankatta
OBJECTIVES: Human serum protein profiling of the individual infected with multiple dengue virus serotypes for identifying the potential biomarkers and to investigate the cause for the severity of dengue virus infection. METHODS: Dengue virus NS1 positive serum samples were pooled into two groups (S2 and S3) based on the molecular serotyping and number of heterotypic infections. The pooled serum samples were subjected to two dimensional gel electrophoresis (2DGE) to identify the differentially expressed proteins...
July 16, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28706387/comparison-of-nonstructural-protein-1-antigen-detection-by-rapid-and-enzyme-linked-immunosorbent-assay-test-and-its-correlation-with-polymerase-chain-reaction-for-early-diagnosis-of-dengue
#15
Seema Gaikwad, Sandhya S Sawant, Jayanthi S Shastri
INTRODUCTION: Early diagnosis of dengue is important for appropriate clinical management and vector control. Different serological tests based on the principle of immunochromatography and enzyme-linked immunosorbent assay (ELISA) are commonly used for detection of antigen and antibodies of dengue virus. The performance of these tests depends on the sensitivity and specificity. Hence, the study was undertaken to compare nonstructural protein-1 (NS1) antigen detection by rapid and ELISA with real-time polymerase chain reaction (RT-PCR) for diagnosis of dengue...
July 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28690377/dengue-symptoms-significance-in-anti-dengue-drug-development-road-less-travelled
#16
Anubrata Paul, Arpana Vibhuti
Dengue outbreak has affected rural areas of Delhi-NCR, Haryana widely but it lacks in surveillance. High cases of dengue symptoms were reported in these regions whereas dengue symptoms have been a neglected issue in the anti-dengue drug development. Therefore, this study aims to analyze the status of the dengue infection, a rural issue of Delhi-NCR, Haryana and to identify the significance of dengue symptoms in anti-dengue drug development. The study was conducted when there is high chance of dengue infection i...
2017: Bioinformation
https://www.readbyqxmd.com/read/28683141/isolation-of-serotype-specific-antibodies-against-dengue-virus-non-structural-protein-1-using-phage-display-and-application-in-a-multiplexed-serotyping-assay
#17
Kebaneilwe Lebani, Martina L Jones, Daniel Watterson, Andrea Ranzoni, Renee J Traves, Paul R Young, Stephen M Mahler
The multidimensional nature of dengue virus (DENV) infections, which can be caused by four distinct serotypes of the virus, complicates the sensitivity of assays designed for the diagnosis of infection. Different viral markers can be optimally detected at different stages of infection. Of particular clinical importance is the early identification of infection, which is pivotal for disease management and the development of blood screening assays. Non-structural protein 1 (NS1) is an early surrogate marker of infection and its detection in serum coincides with detectable viraemia...
2017: PloS One
https://www.readbyqxmd.com/read/28681825/co-circulation-of-all-four-dengue-virus-serotypes-first-report-from-odisha
#18
Baijayantimala Mishra, Jyotirmayee Turuk, Subhra Jyoti Sahu, Atul Khajuria, Subrat Kumar, Anupam Dey, Ashok Kumar Praharaj
The present report describes the detection of co-circulation of all the four dengue serotypes along with rarely detected dengue viruses (DENVs)-4 for the first time in Odisha. One hundred and forty-eight blood samples were tested for dengue NS1 antigen ELISA and IgM antibody (Ab), and twenty early samples were subjected for type-specific multiplex reverse transcription-polymerase chain reaction (RT-PCR). Twenty-three samples found positive for dengue NS1 and/or IgM Ab; five were positive by RT-PCR. DENV-4 was detected in one sample, DENV-2 in two and 2 were co-infected with DENV-1 and 3...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28681816/clinical-and-laboratory-profile-of-dengue-viral-infections-in-and-around-mangalore-india
#19
Tina Damodar, Meena Dias, Reeta Mani, K A Shilpa, Ashwini Manoor Anand, V Ravi, Jutang Tiewsoh
BACKGROUND: Dengue (DEN) is being recognised as the world's major emerging tropical disease. Clinically, DEN may resemble other infections such as malaria, leptospirosis, and typhoid, and thus, laboratory investigations are required for definitive diagnosis. Secondary DEN infection, caused most often by dengue virus (DENV) serotypes 2 and 3, is known to present with severe disease manifestations. This study was undertaken to examine the clinical and laboratory profile of DEN viral infections and to determine the circulating serotypes in and around Mangalore, India...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28648850/complete-genome-analysis-of-dengue-virus-type-3-isolated-from-the-2013-dengue-outbreak-in-yunnan-china
#20
Xiaodan Wang, Dehong Ma, Xinwei Huang, Lihua Li, Duo Li, Yujiao Zhao, Lijuan Qiu, Yue Pan, Junying Chen, Juemin Xi, Xiyun Shan, Qiangming Sun
In the past few decades, dengue has spread rapidly and is an emerging disease in China. An unexpected dengue outbreak occurred in Xishuangbanna, Yunnan, China, resulting in 1331 patients in 2013. In order to obtain the complete genome information and perform mutation and evolutionary analysis of causative agent related to this largest outbreak of dengue fever. The viruses were isolated by cell culture and evaluated by genome sequence analysis. Phylogenetic trees were then constructed by Neighbor-Joining methods (MEGA6...
June 23, 2017: Virus Research
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