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Dengue NS1

Mostafa Heydari, Maliheh Metanat, Mohammad-Ali Rouzbeh-Far, Seyed Mehdi Tabatabaei, Mohammad Rakhshani, Nahid Sepehri-Rad, Maryam Keshtkar-Jahromi
Dengue fever (DF) is a mosquito-borne acute viral disease presenting with hemorrhagic manifestations in severe cases. Southeast Iran is in close proximity to Pakistan, an endemic country for DF. This cross-sectional study was conducted in the Sistan and Baluchestan province in the southeast of Iran to investigate possibility of DF (immunoglobulin M [IgM], immunoglobulin G [IgG], and nonstructural protein 1 [NS1] antigen tests) in 60 clinically suspected patients (April 2013 to August 2015). NS1 protein was detected in 5% ( N = 3), at least one of the antibodies (IgM and/ or IgG) was detected in 11% ( N = 7) of the samples...
March 19, 2018: American Journal of Tropical Medicine and Hygiene
Ramiro Manzano Núñez, James Alejandro Zapata, Herney A García-Perdomo, Diego A Gomez, Mónica A Solís Velasco
INTRODUCTION: Few reports are available about perinatal dengue, with controversial results in regards the risk of perinatal outcome. OBJECTIVE: To report a case of perinatal dengue as a differential diagno sis with neonatal sepsis, which must be considered in endemic areas. CLINICAL CASE: Male newborn of a 23 year-old female, who presented a Non-Structural Protein 1 (NS1) antigen positive to dengue at 36 weeks of gestation and negative anti-dengue antibodies...
December 2017: Revista Chilena de Pediatría
Kunal Tewari, Vishal Vishnu Tewari, Ritu Mehta
Background: Dengue is a major health issue with seasonal rise in dengue fever cases imposing an additional burden on hospitals, necessitating bolstering of services in the emergency department, laboratory with creation of additional dengue fever wards. Objectives: To study the clinical and hematological profile of dengue fever cases presenting to a hospital. Methods: Patients with fever and other signs of dengue with either positive NS1 antigen test or IgM or IgG antibody were included...
2018: Mediterranean Journal of Hematology and Infectious Diseases
Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Chom-Kyu Chong, Yanaihara Pinchemel, Francis Reisdörfer, Joyce Brito Coelho, Ronaldo Ferreira Dias, Pan Kee Bae, Zuinara Pereira Maia Gusmão, Hye-Jin Ahn, Ho-Woo Nam
We developed a Rapid Diagnostic Test (RDT) kit for detecting IgG/IgM antibodies against Zika virus (ZIKV) using monoclonal antibodies to the envelope (E) and non-structural protein 1 (NS1) of ZIKV. These proteins were produced using baculovirus expression vector with Sf9 cells. Monoclonal antibodies J2G7 to NS1 and J5E1 to E protein were selected and conjugated with colloidal gold to produce the Zika IgG/IgM RDT kit (Zika RDT). Comparisons with ELISA, plaque reduction neutralization test (PRNT), and PCR were done to investigate the analytical sensitivity of Zika RDT, which resulted in 100% identical results...
February 2018: Korean Journal of Parasitology
G Theillet, A Rubens, F Foucault, P Dalbon, C Rozand, I Leparc-Goffart, F Bedin
The incidence of flavivirus infections has increased dramatically in recent decades in tropical and sub-tropical areas worldwide, affecting hundreds of millions of people each year. Dengue viruses are typically transmitted by mosquitoes and can cause a wide range of symptoms from flu-like fever to organ impairment and death. Although conventional diagnostic tests can provide early diagnosis of acute dengue infections, access to these tests is often limited in developing countries. Consequently, there is an urgent need to develop affordable, simple, rapid, and robust diagnostic tools that can be used at 'Point of Care' settings...
March 10, 2018: Archives of Virology
Kanjana Srisutthisamphan, Krit Jirakanwisal, Suwipa Ramphan, Natthida Tongluan, Atichat Kuadkitkan, Duncan R Smith
Infections with the mosquito-borne dengue virus (DENV) remain a significant public health challenge. In the absence of a commercial therapeutic to treat DENV infection, a greater understanding of the processes of cellular replication is required. The abundant cellular chaperone protein heat shock protein 90 (Hsp90) has been shown to play a proviral role in the replication cycle of several viruses, predominantly through the stabilization of specific viral proteins. To investigate any potential role of Hsp90 in DENV infection the interaction between Hsp90 and DENV proteins was determined through co-immunoprecipitation experiments...
March 9, 2018: Scientific Reports
Paola Mariela Saba Villarroel, Elif Nurtop, Boris Pastorino, Yelin Roca, Jan Felix Drexler, Pierre Gallian, Thomas Jaenisch, Isabelle Leparc-Goffart, Stéphane Priet, Laetitia Ninove, Xavier de Lamballerie
BACKGROUND: Zika virus (ZIKV), was widely reported in Latin America and has been associated with neuropathologies, as microcephaly, but only few seroprevalence studies have been published to date. Our objective was to determine the seroprevalence amongst Bolivian blood donors and estimate the future potential circulation of the virus. METHODOLOGY: A ZIKV seroprevalence study was conducted between December 2016 and April 2017 in 814 asymptomatic Bolivian volunteer blood donors residing in various eco-environments corresponding to contrasting entomological activities...
March 7, 2018: PLoS Neglected Tropical Diseases
N D B Ehelepola, R K G M Rajapaksha, D M U B Dhanapala, T D K Thennekoon, S Ponnamperuma
BACKGROUND: Concurrent presence of dengue hemorrhagic fever (DHF), tropical pyomyositis and septicemia due to methicillin-resistant Staphylococcus aureus (MRSA) in a previously healthy person has never been reported. These three conditions even individually are potentially fatal. "Here we describe a case of a patient contracting dengue and developing DHF along with concurrent pyomyositis likely to be due to MRSA, leading to MRSA septicemia with abscesses formed by MRSA". CASE PRESENTATION: A 44-year old previously healthy Sinhalese man presented on day 3 of the illness with fever, headache, arthralgia and myalgia and watery loose stools...
February 27, 2018: BMC Infectious Diseases
Scott B Halstead
A tetravalent live-attenuated 3-dose vaccine composed of chimeras of yellow fever 17D and the four dengue viruses (CYD, also called Dengvaxia) completed phase 3 clinical testing in over 35,000 children leading to a recommendation that vaccine be administered to >/ = 9 year-olds residing in highly dengue- endemic countries. When clinical trial results were assessed 2 years after the first dose, vaccine efficacy among seropositives was high, but among seronegatives efficacy was marginal. Breakthrough dengue hospitalizations of vaccinated children occurred continuously over a period of 4-5 years post 3rd dose in an age distribution suggesting these children had been vaccinated when seronegative...
February 26, 2018: Human Vaccines & Immunotherapeutics
Shubham Shrivastava, Divya Tiraki, Arundhati Diwan, Sanjay K Lalwani, Meera Modak, Akhilesh Chandra Mishra, Vidya A Arankalle
Dengue is the most common mosquito-borne viral infection in tropical and sub-tropical countries. In recent years, India has reported increased incidences of concurrent infection with multiple serotypes of dengue viruses (DENV). In the present study, we have characterized DENV circulating during a single season of 2016 in Pune, India. A total of 64 serum samples from NS1 ELISA positive dengue patients were used for PCR amplification of CprM region of the viral genome and sequencing. Phylogenetic analysis documented circulation of all the four DENV serotypes with predominance of DENV-2 (40...
2018: PloS One
D Wu, L Z Zhao, Y H Wu, H Zhang, M Zhang, Q Q Tan, H Q Zhou, F C Zhang, J F He
Objective: To investigate the sensitivity and specificity of commercial nonstructural protein 1 (NS1) testing kits for Dengue fever diagnose, and provide the evidence for diagnostic criteria revision. Methods: 300 PCR or virus isolation positive blood samples for dengue virus were collected from sentinel hospitals for dengue surveillance in Guangzhou, Dongguang and Zhongshang from May 2015 to Nov. 2016. At the same time, 308 PCR negative samples for Dengue virus were collected as control group. The information of the sample was collected using questionnaires...
February 6, 2018: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
Sabrina Alessio Camacho, Regivaldo Gomes Sobral Filho, Pedro Henrique Benites Aoki, Carlos Jose Leopoldo Constantino, Alexandre G Brolo
Zika virus (ZIKV) was considered a public health emergency of international concern after the 2015 outbreak. Serological tests based on immunoassay platforms is one of the methods applied on the diagnosis of ZIKV and dengue virus (DENV). However, the high limits of detection (LOD) and the cross-reactivity between ZIKV and DENV are still limitations in immunological tests. In order to tackle these issues, we have designed an immune-specific assay based on surface-enhanced Raman scattering (SERS) nanoprobes. Gold shell-isolated nanoparticles (Au-SHINs) were synthesized with 100 nm Au nanoparticles and 4 nm silica shell thickness coated with Nile Blue (Raman reporter)...
February 7, 2018: ACS Sensors
Tesfaye Gelanew, Elizabeth Hunsperger
BACKGROUND: Dengue, caused by one of the four serologically distinct dengue viruses (DENV-1 to - 4), is a mosquito-borne disease of serious global health significance. Reliable and cost-effective diagnostic tests, along with effective vaccines and vector-control strategies, are highly required to reduce dengue morbidity and mortality. Evaluation studies revealed that many commercially available NS1 antigen (Ag) tests have limited sensitivity to DENV-4 serotype compared to the other three serotypes...
February 6, 2018: Virology Journal
Mohamed Lamine Hafirassou, Laurent Meertens, Claudia Umaña-Diaz, Athena Labeau, Ophelie Dejarnac, Lucie Bonnet-Madin, Beate M Kümmerer, Constance Delaugerre, Philippe Roingeard, Pierre-Olivier Vidalain, Ali Amara
No abstract text is available yet for this article.
January 30, 2018: Cell Reports
Fernando Rosso, Juan C Pineda, Ana M Sanz, Jorge A Cedano, Luis A Caicedo
Dengue fever is a vector-transmitted viral infection. Non-vectorial forms of transmission can occur through organ transplantation. We reviewed medical records of donors and recipients with suspected dengue in the first post-transplant week. We used serologic and molecular analysis to confirm the infection. Herein, we describe four cases of dengue virus transmission through solid organ transplantation. The recipients had positive serology and RT-PCR. Infection in donors was detected through serology. All cases presented with fever within the first week after transplantation...
January 18, 2018: Brazilian Journal of Infectious Diseases
Thanalai Poonsiri, Gareth S A Wright, Michael S Diamond, Lance Turtle, Tom Solomon, Svetlana V Antonyuk
Japanese encephalitis virus (JEV) is a mosquito-transmitted Flavivirus that is closely related to other emerging viral pathogens including dengue, West Nile (WNV) and Zika viruses. JEV infection can result in meningitis and encephalitis, which in severe cases cause permanent brain damage and death. JEV occurs predominantly in rural areas throughout Southeast Asia, the Pacific islands and the Far East, causing around 68,000 cases worldwide each year. In this study, we present a 2.1 Å resolution crystal structure of the C-terminal β-ladder domain of JEV non-structural protein 1 (NS1-C)...
January 17, 2018: Journal of Virology
C J Reynolds, O M Suleyman, A M Ortega-Prieto, J K Skelton, P Bonnesoeur, A Blohm, V Carregaro, J S Silva, E A James, B Maillère, M Dorner, R J Boyton, D M Altmann
Zika virus (ZIKV) Infection has several outcomes from asymptomatic exposure to rash, conjunctivitis, Guillain-Barré syndrome or congenital Zika syndrome. Analysis of ZIKV immunity is confounded by the fact that several related Flaviviruses infect humans, including Dengue virus 1-4, West Nile virus and Yellow Fever virus. HLA class II restricted T cell cross-reactivity between ZIKV and other Flaviviruses infection(s) or vaccination may contribute to protection or to enhanced immunopathology. We mapped immunodominant, HLA class II restricted, CD4 epitopes from ZIKV Envelope (Env), and Non-structural (NS) NS1, NS3 and NS5 antigens in HLA class II transgenic mice...
January 12, 2018: Scientific Reports
Angel Balmaseda, Jose Victor Zambrana, Damaris Collado, Nadezna Garcia, Saira Saborío, Douglas Elizondo, Juan Carlos Mercado, Karla Gonzalez, Cristhiam Cerpas, Andrea Nuñez, Davide Corti, Jesse J Waggoner, Guillermina Kuan, Raquel Burger-Calderon, Eva Harris
Zika virus (ZIKV) is a mosquito-borne flavivirus that is responsible for recent explosive epidemics in the Americas. Notably, ZIKV infection during pregnancy has been found to cause congenital birth defects, including microcephaly, and ZIKV has been associated with Guillain Barré Syndrome in adults. Diagnosis and surveillance of Zika in the Americas have been challenging due to similar clinical manifestations and extensive antibody cross-reactivity with endemic flaviviral diseases, like dengue. We evaluated four serological and two RT-PCR methods in acute (mean day 1...
January 5, 2018: Journal of Clinical Microbiology
Nasronudin Suharto, Muhammad Amin, Diah Puspita Rini, Achmad Chusnu Romdhoni, Purwaningsih Zulfayandi Pawanis, Muhammad Syamsul Hidayat
Background: Zika virus remained silent for few decades after its first recognition in 1947. Recently, Zika drew global attention due to the outbreak in Pacific island and Latin Americas. The concern was escalating given the shared vector of Zika and dengue with possible deleterious complications. Surabaya as one of the largest cities in Indonesia with high mobility of people is endemic to dengue. This study was aimed to detect Zika infection during dengue outbreaks in Surabaya. Materials and Methods: This was a surveillance study involving patients visiting 4 public hospitals and 8 primary health care centres (PHCs)...
2018: African Journal of Infectious Diseases
Madhulika Mistry, Rajesh K Chudasama, Yogesh Goswami, Chintan Dalwadi, Aarohi Mitra, Garima Mehta
Background: Dengue is an important vector-borne disease with a spectrum of classical fever to hemorrhagic fever to dengue shock syndrome. The present study was conducted with objective to study epidemiological and demographic characteristics of dengue infections during the year 2015 in Saurashtra region, Gujarat state, India. Materials and Methods: The study was conducted at Tertiary Care Hospital, Rajkot, during the year 2015. A total of 3312 blood samples were collected and tested for dengue NS1 antigen and IgM antibody by capture ELISA testing from various districts of Saurashtra region...
April 2017: Journal of Family Medicine and Primary Care
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