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Dengue NS1

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https://www.readbyqxmd.com/read/29158907/chikungunya-outbreak-in-delhi-india-2016-report-on-coinfection-status-and-comorbid-conditions-in-patients
#1
N Kaur, J Jain, A Kumar, M Narang, M K Zakaria, A Marcello, D Kumar, R Gaind, S Sunil
Chikungunya fever is a major public health issue in India affecting billions. After 2010, the infection was in a decline until in 2016, when a massive outbreak affected the country. In this report, we present serologic and molecular investigations of 600 patient samples for chikungunya and dengue viruses along with clinical and comorbidity features. We recruited 600 patients during this outbreak and evaluated them for chikungunya and dengue virus antibodies and virus RNA through IgM, NS1 antigen and quantitative real-time PCR (qPCR)...
November 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/29141913/multiplexed-biomarker-panels-discriminate-zika-and-dengue-virus-infection-in-humans
#2
Guang Song, Hee-Sool Rho, Jianbo Pan, Pedro Ramos, Ki-Jun Yoon, Freddy A Medina, Emily M Lee, Daniel J Eichinger, Guo-Li Ming, Jorge L Muñoz-Jordan, Hengli Tang, Ignacio Pino, Hongjun Song, Jiang Qian, Heng Zhu
Zika virus (ZIKV) and dengue virus (DENV) are closely related flaviviruses that cause widespread, acute febrile illnesses, notably microcephaly for fetuses of infected pregnant women. Detecting the viral cause of these illnesses is paramount to determine risks to patients, counsel pregnant women, and help fight outbreaks. A combined diagnostic algorithm for ZIKV and DENV requires Reverse transcription polymerase chain reaction (RT-PCR) and IgM antibody detection. RT-PCR differentiates between DENV and ZIKV infections during the acute phases of infection, but differentiation based on IgM antibodies is currently nearly impossible in endemic areas...
November 15, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29140541/regulatory-t-cells-in-acute-dengue-viral-infection
#3
Eresha Jayaratne, Dulharie Wijeratne, Samitha Fernando, Achala Kamaladasa, Laksiri Gomes, Ananda Wijewickrama, Graham Stuart Ogg, Gathsaurie Neelika Malavige
Although regulatory T cells (Tregs) have been shown to be expanded in acute dengue, their role in pathogenesis and their relationship to clinical disease severity, and extent of viraemia have not been fully evaluated. The frequency of Tregs was assessed in 56 adult patients with acute dengue, by determining the proportion of FOXP3 expressing CD4(+) CD25(+) T cells (FOXP3+ cells). DENV viral loads were measured by quantitative real time PCR and DENV specific T cell responses were measured by ex vivo IFNγ ELISpot assays to overlapping peptide pools of DENV-NS3, NS1 and NS5...
November 15, 2017: Immunology
https://www.readbyqxmd.com/read/29121099/dengue-virus-ns1-cytokine-independent-vascular-leak-is-dependent-on-endothelial-glycocalyx-components
#4
Dustin R Glasner, Kalani Ratnasiri, Henry Puerta-Guardo, Diego A Espinosa, P Robert Beatty, Eva Harris
Dengue virus (DENV) is the most prevalent, medically important mosquito-borne virus. Disease ranges from uncomplicated dengue to life-threatening disease, characterized by endothelial dysfunction and vascular leakage. Previously, we demonstrated that DENV nonstructural protein 1 (NS1) induces endothelial hyperpermeability in a systemic mouse model and human pulmonary endothelial cells, where NS1 disrupts the endothelial glycocalyx-like layer. NS1 also triggers release of inflammatory cytokines from PBMCs via TLR4...
November 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29116169/a-zika-vaccine-targeting-ns1-protein-protects-immunocompetent-adult-mice-in-a-lethal-challenge-model
#5
Aaron C Brault, Arban Domi, Erin M McDonald, Dalit Talmi-Frank, Nathanael McCurley, Rahul Basu, Harriet L Robinson, Michael Hellerstein, Nisha K Duggal, Richard A Bowen, Farshad Guirakhoo
Zika virus (ZIKV) is a mosquito-borne flavivirus that has rapidly extended its geographic range around the world. Its association with abnormal fetal brain development, sexual transmission, and lack of a preventive vaccine have constituted a global health concern. Designing a safe and effective vaccine requires significant caution due to overlapping geographical distribution of ZIKV with dengue virus (DENV) and other flaviviruses, possibly resulting in more severe disease manifestations in flavivirus immune vaccinees such as Antibody-Dependent Enhancement (ADE, a phenomenon involved in pathogenesis of DENV, and a risk associated with ZIKV vaccines using the envelope proteins as immunogens)...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29107414/kinetics-of-anti-zikv-antibodies-after-zika-infection-using-two-commercial-enzyme-linked-immunoassays
#6
Christophe Pasquier, Guillaume Joguet, Catherine Mengelle, Sabine Chapuy-Regaud, Lynda Pavili, Nadia Prisant, Jacques Izopet, Louis Bujan, Jean-Michel Mansuy
High performance assays are essential for the serological diagnosis of recent and past Zika virus (ZIKV) infections but few are presently available. We used two commercially available NS1 antigen-based enzyme-linked immunoassays to study the kinetics of anti-ZIKV IgM and IgG in 15 ZIKV-infected patients for up to 180days after clinical onset. The Diapro assay detected anti-ZIKV IgM reactivity more frequently (100%) and for longer (median 53days) than did the Euroimmun assay (60%; 13days, P<0.005). Both assays detected anti-ZIKV IgG reactivity 11days after clinical onset in all subjects...
September 15, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29098002/serum-ferritin-a-backstage-weapon-in-diagnosis-of-dengue-fever
#7
Soumyabrata Roy Chaudhuri, Subhayan Bhattacharya, Mainak Chakraborty, Kingshuk Bhattacharjee
Aims: This retrospective study evaluates ferritin as a surrogate marker for dengue infection (NS1 and IgM negative stage) as opposed to other febrile illnesses of infective or inflammatory etiology (OFI). Methodology: Data of all patients admitted to medical ward and medical ITU during the dengue outbreak were collected. Patients admitted between 5 and 10 days of febrile illness without a diagnosis were included. Patients with NS1 positivity (Days 2-8) and/or positive IgM for dengue (Days 6-10) were considered to be dengue cases and those with other confirmed diagnoses were considered in the OFI group...
2017: Interdisciplinary Perspectives on Infectious Diseases
https://www.readbyqxmd.com/read/29076677/pattern-of-presentation-among-adults-hospitalized-with-dengue-disease
#8
Abdul Rauf, Nasir Hussain Shah Kazmi, Haidar Zaman, Saima Gillani, Tariq Shah, Faizan Malik, Shoaib Ismaeel
BACKGROUND: Dengue is a mosquito born viral infection that has rapidly spread in the world particularly in Southeast Asia. The aim of this hospital based study was to see the demographic, clinical and laboratory characteristics in adults with dengue infection in Hazara region of Northern Pakistan. METHODS: This is a descriptive study. Clinical, laboratory and demographic information were collected from adult patients with suspected dengue infection (n=100) and then managed in one of medical units of tertiary care hospital in Abbottabad from August to October 2015...
July 2017: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/29060498/pca-mlp-svm-distinction-of-salivary-raman-spectra-of-dengue-fever-infection
#9
A R M Radzol, Khuan Y Lee, W Mansor, P S Wong, I Looi
Dengue fever (DF) is a disease of major concern caused by flavivirus infection. Delayed diagnosis leads to severe stages, which could be deadly. Of recent, non-structural protein (NS1) has been acknowledged as a biomarker, alternative to immunoglobulins for early detection of dengue in blood. Further, non-invasive detection of NS1 in saliva makes the approach more appealing. However, since its concentration in saliva is less than blood, a sensitive and specific technique, Surface Enhanced Raman Spectroscopy (SERS), is employed...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29056517/molecular-characterization-of-dengue-viruses-isolated-from-patients-in-central-java-indonesia
#10
Endang S Kusmintarsih, Rahma F Hayati, Oktaviani N Turnip, Benediktus Yohan, Suhestri Suryaningsih, Hery Pratiknyo, Dionisius Denis, R Tedjo Sasmono
BACKGROUND: Dengue is hyper-endemic in Indonesia. Purwokerto city in Central Java province is routinely ravaged by the disease. Despite the endemicity of dengue in this city, there is still no data on the virological aspects of dengue in the city. We conducted a molecular surveillance study of the circulating dengue viruses (DENV) in Purwokerto city to gain information on the virus origin, serotype and genotype distribution, and phylogenetic characteristics of DENV. METHODS: A cross-sectional dengue molecular surveillance study was conducted in Purwokerto...
October 19, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/29021087/evaluation-of-onestep-dengue-ns1-rapidip%C3%A2-instatest-and-onestep-dengue-fever-igg-igm-rapicard%C3%A2-instatest-during-the-course-of-a-dengue-type-1-epidemic
#11
Ivan Vickers, Kevin Harvey, Kereann Nelson, Michelle Brown, Marion Bullock-DuCasse, John Lindo
We determined the diagnostic performance of the OneStep NS1 and the OneStep IgG/IgM RDT kits against a panel of samples which comprised of 174 dengue positive and 165 dengue negative sera characterized by three reference enzyme-linked immunosorbent assays (ELISAs). The diagnostic sensitivities of the OneStep kits for the detection of individual biomarkers of NS1, IgM and IgG were 90% (95% CI: 82.1-94.7), 32.4% (95% CI: 24.8-40.8) and 44.4% (95% CI: 38.2-50.7), respectively. The combination of the OneStep IgG/IgM kit with the OneStep NS1 kit demonstrated significantly higher sensitivities for the combined NS1/IgM (96...
August 26, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29020159/distinguishing-secondary-dengue-virus-infection-from-zika-virus-infection-with-previous-dengue-by-a-combination-of-three-simple-serological-tests
#12
Wen-Yang Tsai, Han Ha Youn, Carlos Brites, Jih-Jin Tsai, Jasmine Tyson, Celia Pedroso, Jan Felix Drexler, Mars Stone, Graham Simmons, Michael P Busch, Marion Lanteri, Susan L Stramer, Angel Balmaseda, Eva Harris, Wei-Kung Wang
Background: The explosive spread of Zika virus (ZIKV) and associated microcephaly present an urgent need for sensitive and specific serodiagnostic tests, particularly for pregnant women in dengue virus (DENV)-endemic regions. Recent reports of enhanced ZIKV replication by dengue-immune sera have raised concerns about the role of previous DENV infection on the risk and severity of microcephaly and other ZIKV complications. Methods: Enzyme-linked immunosorbent assays (ELISAs) based on ZIKV and DENV non-structural protein 1 (NS1) were established to test acute, convalescent-phase and post-convalescent-phase serum/plasma samples from reverse-transcription polymerase-chain reaction-confirmed-cases including 20 primary ZIKV, 25 ZIKV with previous dengue, 58 secondary DENV, and 16 primary DENV1 infections...
July 29, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28991176/innate-immune-evasion-mediated-by-flaviviridae-non-structural-proteins
#13
REVIEW
Shun Chen, Zhen Wu, Mingshu Wang, Anchun Cheng
Flaviviridae-caused diseases are a critical, emerging public health problem worldwide. Flaviviridae infections usually cause severe, acute or chronic diseases, such as liver damage and liver cancer resulting from a hepatitis C virus (HCV) infection and high fever and shock caused by yellow fever. Many researchers worldwide are investigating the mechanisms by which Flaviviridae cause severe diseases. Flaviviridae can interfere with the host's innate immunity to achieve their purpose of proliferation. For instance, dengue virus (DENV) NS2A, NS2B3, NS4A, NS4B and NS5; HCV NS2, NS3, NS3/4A, NS4B and NS5A; and West Nile virus (WNV) NS1 and NS4B proteins are involved in immune evasion...
October 7, 2017: Viruses
https://www.readbyqxmd.com/read/28985612/a-dual-marker-label-free-electrochemical-assay-for-flavivirus-dengue-diagnosis
#14
Adriano Santos, Paulo R Bueno, Jason J Davis
Dengue is a RNA viral illness of the genus Flavivirus which can cause, depending on the pervasiveness of the infection, hemorrhagic dengue fever or dengue shock syndrome. Herein we present an electrochemical label free approach enabling the rapid sensitive quantification of NS1 and IgG (supporting an ability to distinguish primary and secondary infections). Using a bifunctional SAM containing PEG moieties and a tethered redox thiol, both markers are detectable across clinically relevant levels by label free impedance derived redox capacitance...
September 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28978319/acute-undifferentiated-fever-in-india-a-multicentre-study-of-aetiology-and-diagnostic-accuracy
#15
Kristine Mørch, Anand Manoharan, Sara Chandy, Novin Chacko, Gerardo Alvarez-Uria, Suvarna Patil, Anil Henry, Joel Nesaraj, Cijoy Kuriakose, Ashita Singh, Siby Kurian, Christel Gill Haanshuus, Nina Langeland, Bjørn Blomberg, George Vasanthan Antony, Dilip Mathai
BACKGROUND: The objectives of this study were to determine the proportion of malaria, bacteraemia, scrub typhus, leptospirosis, chikungunya and dengue among hospitalized patients with acute undifferentiated fever in India, and to describe the performance of standard diagnostic methods. METHODS: During April 2011-November 2012, 1564 patients aged ≥5 years with febrile illness for 2-14 days were consecutively included in an observational study at seven community hospitals in six states in India...
October 4, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28966494/nine-year-trends-of-dengue-virus-infection-in-mumbai-western-india
#16
Jayanthi Shastri, Manita Williamson, Nilima Vaidya, Sachee Agrawal, Om Shrivastav
INTRODUCTION: Dengue virus (DENV) causes a wide range of diseases in humans, from acute febrile illness Dengue fever (DF) to life-threatening Dengue hemorrhagic fever (DHF) or Dengue shock syndrome (DSS). Factors believed to be responsible for spread of Dengue virus infection include explosive population growth, unplanned urban overpopulation with inadequate public health systems, poor standing water and vector control, climate changes and increased international recreational, business, military travel to endemic areas...
October 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28956770/genome-wide-mutagenesis-of-dengue-virus-reveals-plasticity-of-the-ns1-protein-and-enables-generation-of-infectious-tagged-reporter-viruses
#17
Nicholas S Eyre, Stephen M Johnson, Auda A Eltahla, Maria Aloi, Amanda L Aloia, Christopher A McDevitt, Rowena A Bull, Michael R Beard
Dengue virus (DENV) is a major global pathogen that causes significant morbidity and mortality in tropical and subtropical areas worldwide. An improved understanding of the regions within the DENV genome and its encoded proteins that are required for the virus replication cycle will expedite the development of urgently required therapeutics and vaccines. We subjected an infectious DENV genome to unbiased insertional mutagenesis and used next-generation sequencing to identify sites that tolerate 15-nucleotide insertions during the virus replication cycle in hepatic cell culture...
December 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28954927/rapid-antigen-tests-for-dengue-virus-serotypes-and-zika-virus-in-patient-serum
#18
Irene Bosch, Helena de Puig, Megan Hiley, Marc Carré-Camps, Federico Perdomo-Celis, Carlos F Narváez, Doris M Salgado, Dewahar Senthoor, Madeline O'Grady, Elizabeth Phillips, Ann Durbin, Diana Fandos, Hikaru Miyazaki, Chun-Wan Yen, Margarita Gélvez-Ramírez, Rajas V Warke, Lucas S Ribeiro, Mauro M Teixeira, Roque P Almeida, José E Muñóz-Medina, Juan E Ludert, Mauricio L Nogueira, Tatiana E Colombo, Ana C B Terzian, Patricia T Bozza, Andrea S Calheiros, Yasmine R Vieira, Giselle Barbosa-Lima, Alexandre Vizzoni, José Cerbino-Neto, Fernando A Bozza, Thiago M L Souza, Monique R O Trugilho, Ana M B de Filippis, Patricia C de Sequeira, Ernesto T A Marques, Tereza Magalhaes, Francisco J Díaz, Berta N Restrepo, Katerine Marín, Salim Mattar, Daniel Olson, Edwin J Asturias, Mark Lucera, Mohit Singla, Guruprasad R Medigeshi, Norma de Bosch, Justina Tam, Jose Gómez-Márquez, Charles Clavet, Luis Villar, Kimberly Hamad-Schifferli, Lee Gehrke
The recent Zika virus (ZIKV) outbreak demonstrates that cost-effective clinical diagnostics are urgently needed to detect and distinguish viral infections to improve patient care. Unlike dengue virus (DENV), ZIKV infections during pregnancy correlate with severe birth defects, including microcephaly and neurological disorders. Because ZIKV and DENV are related flaviviruses, their homologous proteins and nucleic acids can cause cross-reactions and false-positive results in molecular, antigenic, and serologic diagnostics...
September 27, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28934991/the-dengue-preface-to-endemic-in-mainland-china-the-historical-largest-outbreak-by-aedes-albopictus-in-guangzhou-2014
#19
Lei Luo, Li-Yun Jiang, Xin-Cai Xiao, Biao Di, Qin-Long Jing, Sheng-Yong Wang, Jin-Ling Tang, Ming Wang, Xiao-Ping Tang, Zhi-Cong Yang
BACKGROUND: Dengue was regarded as a mild epidemic in mainland China transmitted by Aedes albopictus. However, the 2014 record-breaking outbreak in Guangzhou could change the situation. In order to provide an early warning of epidemic trends and provide evidence for prevention and control strategies, we seek to characterize the 2014 outbreak through application of detailed cases and entomological data, as well as phylogenetic analysis of viral envelope (E) gene. METHODS: We used case survey data identified through the Notifiable Infectious Disease Report System, entomological surveillance and population serosurvey, along with laboratory testing for IgM/IgG, NS1, and isolation of viral samples followed by E gene sequencing and phylogenetic analysis to examine the epidemiological and molecular characteristics of the outbreak...
September 22, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28916391/zika-virus-structural-biology-and-progress-in-vaccine-development
#20
REVIEW
Hsiao-Han Lin, Bak-Sau Yip, Li-Min Huang, Suh-Chin Wu
The growing number of zika virus (ZIKV) infections plus a 20-fold increase in neonatal microcephaly in newborns in Brazil have raised alarms in many countries regarding the threat to pregnant women. Instances of microcephaly and central nervous system malformations continue to increase in ZIKV outbreak regions. ZIKV is a small enveloped positive-strand RNA virus belonging to the Flavivirus genus of the Flaviviridae family. High-resolution ZIKV structures recently identified by cryo-electron microscopy indicate that the overall ZIKV structure is similar to those of other flaviviruses...
September 12, 2017: Biotechnology Advances
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