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Dengue NS1

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https://www.readbyqxmd.com/read/29768549/evaluating-the-use-of-fluorescence-based-flow-cytometry-assay-for-dengue-diagnosis-using-peripheral-blood-mononuclear-cells
#1
Luzia Aparecida Costa Barreira, Priscila Santos Scheucher, Marilia Farignoli Romeiro, Leonardo La Serra, Soraya Jabur Badra, William Marciel de Souza, Luiz Tadeu Moraes Figueiredo
INTRODUCTION: Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year. METHODS: In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: All three assays identified 29...
March 2018: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/29759074/dengue-fever-complicated-with-guillain-barr%C3%A3-syndrome-a-case-report-and-review-of-the-literature
#2
Chamara Dalugama, John Shelton, Mahendra Ekanayake, Indika Bandara Gawarammana
BACKGROUND: Dengue is an arboviral infection classically presenting with fever, arthralgia, headache, and rashes. It is hyperendemic in Sri Lanka and has a major impact on health. Neurological complications of dengue fever are rare but have been reported in the literature. CASE PRESENTATION: A 60-year-old Sri Lankan man presented with a history of fever, arthralgia, and generalized malaise of 2 days duration. A diagnosis of dengue was confirmed with leukopenia, thrombocytopenia, and positive NS1 antigen done on day 2 without evidence of hemoconcentration...
May 15, 2018: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/29751761/evaluation-of-an-immunochromatography-rapid-diagnosis-kit-for-detection-of-chikungunya-virus-antigen-in-india-a-dengue-endemic-country
#3
Jaspreet Jain, Tamaki Okabayashi, Navjot Kaur, Emi Nakayama, Tatsuo Shioda, Rajni Gaind, Takeshi Kurosu, Sujatha Sunil
BACKGROUND: Chikungunya virus (CHIKV) and dengue virus (DENV) are arboviruses that share the same Aedes mosquito vector, and there is much overlap in endemic areas. In India, co-infection with both viruses is often reported. Clinical manifestations of Chikungunya fever is often confused with dengue fever because clinical symptoms of both infections are similar. It is, therefore, difficult to differentiate from those of other febrile illnesses, especially dengue fever. We previously developed a CHIKV antigen detection immunochromatography (IC) rapid diagnosis kit [1]...
May 11, 2018: Virology Journal
https://www.readbyqxmd.com/read/29751200/biosensor-based-selective-detection-of-zika-virus-specific-antibodies-in-infected-individuals
#4
Gustavo Cabral-Miranda, Ana R Cardoso, Luis C S Ferreira, M Goreti F Sales, Martin F Bachmann
Zika virus (ZIKV) recently emerged as a global threat subsequent to its global spread because it induces microencephaly and other brain damages in infants born to infected mothers. Epidemiological monitoring of infection has been hampered by the absence of reliable serological tests capable to distinguish between ZIKV and other Flavivirus infections, in particular Dengue virus (DENV). As both viruses are transmitted by the same mosquito-species, their distributions largely overlap and reliable serological distinction between the viruses is essential...
May 1, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29735824/molecular-characterisation-and-phylogenetic-analysis-of-dengue-outbreak-in-pasighat-arunachal-pradesh-northeast-india
#5
Biswajyoti Borkakoty, Mandakini Das, Kishore Sarma, Aniruddha Jakharia, Palash Kumar Das, Chandrakanta Bhattacharya, Basumoti Apum, Dipankar Biswas
Background and Objectives: Dengue is one of the most prevalent arboviral diseases in the world with 390 million dengue infections per year. In this study, we report the molecular characterisation of dengue outbreak in Pasighat, Arunachal Pradesh, Northeast India during 2015. Subjects and Methods: : A total of 613 dengue-suspected cases were screened for dengue virus by dengue NS1 Ag and anti-dengue IgM antibody depending on the duration of sample collection and onset of symptom...
January 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29720514/secretion-of-the-non-structural-protein-1-of-dengue-virus-from-infected-mosquito-cells-facts-and-speculations
#6
Ana C Alcalá, Laura A Palomares, Juan E Ludert
The dengue virus NS1 is a multifunctional glycoprotein. For decades, the notion in the field was that NS1 was secreted exclusively from vertebrate, but not mosquito, cells. However, recent evidence shows that mosquito cells also secrete NS1 efficiently. In this review, we discuss the evidence for dengue, and other flaviviruses, NS1 secretion from mosquito cells, differences between NS1 secreted from mosquito and vertebrate cells, and possible roles of soluble NS1 in the insect flavivirus vector.
May 2, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29709965/a-multicenter-study-of-clinical-presentations-and-predictive-factors-for-severe-manifestation-of-dengue-in-adults
#7
Saranya Temprasertrudee, Vipa Thanachartwet, Varunee Desakorn, Jiraporn Keatkla, Wasun Chantratita, Sasisopin Kiertiburanakul
Severe dengue is more prevalent in adults than in children. Our objectives were to determine the clinical presentations of dengue in adults and to identify predictive factors for severe dengue. A retrospective cohort study was performed in adults with dengue, as confirmed by positive NS1 antigen. Patients were classified into non-severe and severe dengue. A total of 357 patients were enrolled; 45.4% were male, with median (interquartile range, IQR) age of 27.9 (21.8-43.5) years. In all, 28.3% of patients had warning signs and 10...
April 27, 2018: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29702687/macrophage-migration-inhibitory-factor-is-critical-for-dengue-ns1-induced-endothelial-glycocalyx-degradation-and-hyperpermeability
#8
Hong-Ru Chen, Chiao-Hsuan Chao, Ching-Chuan Liu, Tzong-Shiann Ho, Huey-Pin Tsai, Guey-Chuen Perng, Yee-Shin Lin, Jen-Ren Wang, Trai-Ming Yeh
Vascular leakage is one of the salient characteristics of severe dengue. Nonstructural protein 1 (NS1) of dengue virus (DENV) can stimulate endothelial cells to secrete endothelial hyperpermeability factor, macrophage migration inhibitory factor (MIF), and the glycocalyx degradation factor heparanase 1 (HPA-1). However, it is unclear whether MIF is directly involved in NS1-induced glycocalyx degradation. In this study, we observed that among NS1, MIF and glycocalyx degradation-related molecules, the HPA-1, metalloproteinase 9 (MMP-9) and syndecan 1 (CD138) serum levels were all increased in dengue patients, and only NS1 and MIF showed a positive correlation with the CD138 level in severe patients...
April 27, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29699483/emergence-of-the-asian-lineage-dengue-virus-type-3-genotype-iii-in-malaysia
#9
Kim-Kee Tan, Nurul-Izzani Zulkifle, Syuhaida Sulaiman, Sui-Ping Pang, NurAsyura NorAmdan, NorAziyah MatRahim, Juraina Abd-Jamil, Meng-Hooi Shu, Nor Muhammad Mahadi, Sazaly AbuBakar
BACKGROUND: Dengue virus type 3 genotype III (DENV3/III) is associated with increased number of severe infections when it emerged in the Americas and Asia. We had previously demonstrated that the DENV3/III was introduced into Malaysia in the late 2000s. We investigated the genetic diversity of DENV3/III strains recovered from Malaysia and examined their phylogenetic relationships against other DENV3/III strains isolated globally. RESULTS: Phylogenetic analysis revealed at least four distinct DENV3/III lineages...
April 24, 2018: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/29696069/could-homeopathy-become-an-alternative-therapy-in-dengue-fever-an-example-of-10-case-studies
#10
Seema Mahesh, Mallappa Mahesh, George Vithoulkas
Background: Dengue fever is one of the most rampant epidemics in India of late and any therapy that may help limit the sickness and hospital admissions is worth considering. In India complementary and alternative medicine physicians are medically trained and hence have a role to play in delivery of public health. Case Series: We present a retrospective case series of 10 Indian patients who were diagnosed with dengue fever and treated exclusively with homeopathic remedies at Bangalore, India. This case series demonstrates with evidence of laboratory reports that even when the platelets dropped considerably there was good result without resorting to any other means...
January 2018: Journal of Medicine and Life
https://www.readbyqxmd.com/read/29684416/development-of-antibody-biomarkers-of-long-term-and-recent-dengue-virus-infections
#11
Eduardo J M Nascimento, James W Huleatt, Marli T Cordeiro, Priscila M S Castanha, James K George, Eduard Grebe, Alex Welte, Monique Brown, Donald S Burke, Ernesto T A Marques
Dengue virus (DENV) infections elicit antibody responses to the non-structural protein 1 (NS1) that are associated with protection against disease. However, the antibody isotypes and subclasses involved, and their kinetics have not been extensively studied. We characterized the antibody responses to DENV NS1 by enzyme-linked immunosorbent assay (ELISA) in a longitudinal cohort of 266 confirmed dengue cases in Recife, Northeast Brazil. Samples were collected during the febrile phase and up to over 3 years after onset of symptoms...
April 20, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29678172/validity-of-international-classification-of-diseases-icd-coding-for-dengue-infections-in-hospital-discharge-records-in-malaysia
#12
Yuan-Liang Woon, Keng-Yee Lee, Siti Fatimah Zahra Mohd Anuar, Pik-Pin Goh, Teck-Onn Lim
BACKGROUND: Hospitalization due to dengue illness is an important measure of dengue morbidity. However, limited studies are based on administrative database because the validity of the diagnosis codes is unknown. We validated the International Classification of Diseases, 10th revision (ICD) diagnosis coding for dengue infections in the Malaysian Ministry of Health's (MOH) hospital discharge database. METHODS: This validation study involves retrospective review of available hospital discharge records and hand-search medical records for years 2010 and 2013...
April 20, 2018: BMC Health Services Research
https://www.readbyqxmd.com/read/29677500/raman-spectral-analysis-for-rapid-screening-of-dengue-infection
#13
T Mahmood, H Nawaz, A Ditta, M I Majeed, M A Hanif, N Rashid, H N Bhatti, H F Nargis, M Saleem, F Bonnier, H J Byrne
Infection with the dengue virus is currently clinically detected according to different biomarkers in human blood plasma, commonly measured by enzyme linked immunosorbent assays, including non-structural proteins (Ns1), immunoglobulin M (IgM) and immunoglobulin G (IgG). However, there is little or no mutual correlation between the biomarkers, as demonstrated in this study by a comparison of their levels in samples from 17 patients. As an alternative, the label free, rapid screening technique, Raman spectroscopy has been used for the characterisation/diagnosis of healthy and dengue infected human blood plasma samples...
April 11, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29661824/development-of-dengue-virus-serotype-specific-ns1-capture-assays-for-the-rapid-and-highly-sensitive-identification-of-the-infecting-serotype-in-human-sera
#14
Katharina Röltgen, Natalie Rose, Alessia Ruggieri, Louisa Warryn, Nicole Scherr, Carlos Augusto Pinho-Nascimento, Marco Tamborrini, Thomas Jaenisch, Gerd Pluschke
Dengue fever can be caused by one of four distinct dengue virus (DENV) serotypes that cocirculate in many parts of the world. Point of care serotype-specific nonstructural protein-1 (NS1) capture assays for the rapid serotyping of DENV in human sera would greatly support epidemiological surveillance and potentially also prognosis in individual patients. To ensure both serotype specificity and broad coverage of variants within serotypes, we have applied an innovative approach for the generation and selection of serotype-specific anti-NS1 mAbs...
April 16, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29655694/tatanan-a-from-the-acorus-calamus-l-root-inhibited-dengue-virus-proliferation-and-infections
#15
Xingang Yao, Yun Ling, Songxin Guo, Wenyu Wu, Shijun He, Qing Zhang, Min Zou, Kutty Selva Nandakumar, Xiaoguang Chen, Shuwen Liu
BACKGROUND: Acorus calamus l. (Acoraceae) is a well-known traditional Chinese medicinal plant, whose root are historically mainly used to treat neurodegenerative diseases, and for cholera treatment. This datum strongly indicates the antimicrobial activity of A. calamus. PURPOSE: Our goal is to find the active constituents of A. calamus to treat dengue virus (DENV) infections, and to study the effects and mechanisms of these active substances. METHODS: The root of A...
March 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29621534/diversity-of-dengue-virus-3-genotype-iii-in-jeddah-saudi-arabia
#16
Anwar M Hashem, Sayed S Sohrab, Sherif A El-Kafrawy, Adly M M Abd-Alla, Saeid Abo El-Ela, Turki S Abujamel, Ahmed M Hassan, Suha A Farraj, Noura A Othman, Remi N Charrel, Esam I Azhar
BACKGROUND: Dengue is the most important arboviral disease in tropical and subtropical countries. Dispersal of the vector and an increase in migratory flow between countries have led to large epidemics and severe clinical outcomes. Over the past 20 years, dengue epidemics have become more wide-spread and frequent. Previous studies have shown that dengue is endemic in Jeddah, Makkah and Al-Madinah in western Saudi Arabia as well as in Jazan region in the southern part of the country. The four serotypes of dengue virus (DENV) have been reported from western Saudi Arabia...
April 2, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29616396/genomic-analysis-of-dengue-virus-serotype-1-denv-1-genotypes-from-surabaya-indonesia
#17
Benediktus Yohan, Puspa Wardhani, Hidayat Trimarsanto, A Aryati, R Tedjo Sasmono
Dengue has caused a significant public health impact globally. With the diverse genetic of the causative viruses, analysis of dengue virus (DENV) genomes is important to supplement epidemiological data with information that can be used to reconstruct the history of epidemics in time and space. We have reported the clinical and virological characteristics of dengue in Surabaya, Indonesia and revealed the presence of all four DENV serotypes and the predominance of DENV-1. The further classification of Surabaya DENV-1 into two different genotypes warrants in-depth genomic analysis to study the dynamics of both genotypes and their contribution to virus evolution, virus transmission, and disease...
April 3, 2018: Virus Genes
https://www.readbyqxmd.com/read/29608995/coat-protein-complex-i-facilitates-dengue-virus-production
#18
Nopprarat Tongmuang, Umpa Yasamut, Pucharee Songprakhon, Thanyaporn Dechtawewat, Shilu Malakar, Sansanee Noisakran, Pa-Thai Yenchitsomanus, Thawornchai Limjindaporn
Dengue hemorrhagic fever is a life-threatening disease caused by the dengue virus (DENV). After DENV enters into host cells, it replicates to generate viral particles to infect other cells. DENV exploits components of the cellular trafficking pathway to achieve effective virion production. Understanding of the proteins required for this trafficking process is essential for revealing the pathogenesis of DENV infection. Coat protein complex and soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), two host protein families in the cellular trafficking pathway, were investigated to elucidate their respective roles during DENV infection...
March 30, 2018: Virus Research
https://www.readbyqxmd.com/read/29607366/blood-group-ab-is-associated-with-severe-forms-of-dengue-virus-infection
#19
Kalamathy Murugananthan, Sivaganesh Subramaniyam, Thirunavukarasu Kumanan, Leigh Owens, Natkunam Ketheesan, Faseeha Noordeen
Several viral and host factors are believed to contribute to the development of severe forms of dengue such as dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS) following a dengue virus (DENV) infection. The pathogenesis of DHF/DSS is not fully understood, however, host factors like ABO blood groups have been shown to contribute to the severity of DENV infection. The present study investigated the association of blood groups with severity of DENV infection in the northern region of Sri Lanka. The blood-groups of 405 patients positive for DENV NS1 antigen and anti-DENV IgM/IgG were determined using the standard haemagglutination assay recommended by the national blood bank/s...
March 2018: Virusdisease
https://www.readbyqxmd.com/read/29607357/simultaneous-detection-and-serotyping-of-dengue-infection-using-single-tube-multiplex-cdc-dengue-real-time-rt-pcr-from-india
#20
Shashi Sharma, Kundan Tandel, Surabhi Danwe, Puneet Bhatt, P K Dash, Praveer Ranjan, K R Rathi, Rajiv Mohan Gupta, M M Parida
Four antigenically different dengue virus serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) are known to cause infections in humans. Some of these are known to cause more severe disease than the others. Chances for developing Dengue hemorrhagic fever-dengue shock syndrome (DHF-DSS) increases significantly with history of previous infection with one of the four serotypes. Therefore, early diagnosis, serotyping and providing early warning of dengue fever epidemics to concerned authorities becomes very important for better patient outcome and to curb the rapid spread in the community...
March 2018: Virusdisease
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