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Dengue NS1

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https://www.readbyqxmd.com/read/28620811/serological-molecular-and-clinical-correlates-of-dengue-from-a-tertiary-care-centre-in-chennai-india
#1
Vigna Seshan, Gopalsamy Sarangan, Khaleefathullah Sheriff, Kaveri Krishnasamy, Gunasekaran Palani, Padma Srikanth
Dengue disease is caused by dengue viruses 1-4 and has been ranked by the World Health Organisation (WHO) as the fastest spreading vector-borne viral disease. Dengue is often underreported and misdiagnosed due to a wide spectrum of clinical manifestations. Diagnosis of dengue is based on clinical case definitions and laboratory methods. Newer case definitions of dengue have been formulated by clinical studies in order to improve case detection. Owing to its epidemic potential, mortality and morbidity, there is a need for a rapid and accurate diagnostic assay for dengue in order to help the clinician in the early detection of cases and to prevent disease progression...
June 16, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28599640/dengue-type-4-in-rio-de-janeiro-brazil-case-characterization-following-its-introduction-in-an-endemic-region
#2
Manoela Heringer, Thiara Manuele A Souza, Monique da Rocha Q Lima, Priscila Conrado G Nunes, Nieli Rodrigues da C Faria, Fernanda de Bruycker-Nogueira, Thaís Chouin-Carneiro, Rita Maria R Nogueira, Flavia Barreto Dos Santos
BACKGROUND: Due to the populations' susceptibility, DENV-4 introduction in 2010 led to the occurrence of explosive epidemics in the following years in Brazil. In 2011, DENV-4 was identified in Rio de Janeiro (RJ) and it was prevalent in 2012 and 2013. Here, we aimed to characterize clinical, epidemiological and laboratorial aspects of DENV-4 cases after this serotype introduction in an endemic scenario. METHODS: Dengue suspected cases (n = 3727) were received and analyzed from January 2011 to December 2013, during outbreaks occurred in RJ, Brazil...
June 9, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28598047/awareness-and-outcome-of-changing-trends-in-clinical-profile-of-dengue-fever-a-retrospective-analysis-of-dengue-epidemic-from-january-to-december-2014-at-a-tertiary-care-hospital
#3
Jitendra Singh, Anju Dinkar, Virendra Atam, D Himanshu, Kamlesh Kumar Gupta, Kauser Usman, Ravi Misra
BACKGROUND: Dengue fever is caused by mosquito-borne arboviral infection that has become a public health concern globally. Recently, an alarming rise of dengue has also been seen in India. Hence the study was undertaken to know profile of clinical manifestations and laboratory findings during the evolution of dengue fever. METHODS: In this study, retrospective data analysis was done in 216 seropositive dengue patients admitted between January to December 2014 in department of medicine at a north Indian care hospital...
May 2017: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/28591408/decrease-in-dengue-virus-2-infection-and-reduction-of-cytokine-chemokine-production-by-uncaria-guianensis-in-human-hepatocyte-cell-line-huh-7
#4
Cíntia da Silva Mello, Ligia Maria Marino Valente, Thiago Wolff, Raimundo Sousa Lima-Junior, Luciana Gomes Fialho, Cintia Ferreira Marinho, Elzinandes Leal Azeredo, Luzia Maria Oliveira-Pinto, Rita de Cássia Alves Pereira, Antonio Carlos Siani, Claire Fernandes Kubelka
BACKGROUND: Dengue fever may present hemorrhages and cavitary effusions as result of exacerbated immune responses. We investigated hydro-alcoholic extracts from leaves (UGL) and bark (UGB) of the medicinal species Uncaria guinanensis with respect to antiviral effects in Dengue virus (DENV) infection and in immunological parameters associated with in vivo physiopathological features. METHODS: Chemical profiles from UGB or UGL were compared in thin layer chromatography and 1H nuclear magnetic resonance using flavonoid compounds and a pentacyclic oxindole alkaloid-enriched fraction as references...
June 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28562637/identification-of-diagnostic-peptide-regions-that-distinguish-zika-virus-from-related-mosquito-borne-flaviviruses
#5
Alexandra J Lee, Roshni Bhattacharya, Richard H Scheuermann, Brett E Pickett
Zika virus (ZIKV) is a member of the Flavivirus genus of positive-sense single-stranded RNA viruses, which includes Dengue, West Nile, Yellow Fever, and other mosquito-borne arboviruses. Infection by ZIKV can be difficult to distinguish from infection by other mosquito-borne Flaviviruses due to high sequence similarity, serum antibody cross-reactivity, and virus co-circulation in endemic areas. Indeed, existing serological methods are not able to consistently differentiate ZIKV from other Flaviviruses, which makes it extremely difficult to accurately calculate the incidence rate of Zika-associated Guillain-Barre in adults, microcephaly in newborns, or asymptomatic infections within a geographical area...
2017: PloS One
https://www.readbyqxmd.com/read/28544099/genetic-signatures-coupled-with-lineage-shift-characterize-endemic-evolution-of-dengue-virus-serotype-2-during-2015-outbreak-in-delhi-india
#6
Manish Chandra Choudhary, Ekta Gupta, Shvetank Sharma, Nadeem Hasnain, Pragya Agarwala
OBJECTIVE: In 2015 New Delhi witnessed a massive outbreak of Dengue virus (DENV) resulting in high morbidity and mortality. We report the molecular characterization of the dominant circulating DENV strain to understand its evolution and dispersal. MATERIALS AND METHODS: DENV infections were diagnosed by detection of IgM/NS1 antigen and serotyping was performed by C-PrM PCR. Envelope gene was amplified and variation(s) in envelope gene were analyzed. Phylogenetic tree construction, time-based phylogeny and origin of DENV were analyzed...
May 23, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28531223/dengue-in-bali-clinical-characteristics-and-genetic-diversity-of-circulating-dengue-viruses
#7
Dewi Megawati, Sri Masyeni, Benediktus Yohan, Asri Lestarini, Rahma F Hayati, Febrina Meutiawati, Ketut Suryana, Tangking Widarsa, Dewa G Budiyasa, Ngurah Budiyasa, Khin S A Myint, R Tedjo Sasmono
A high number of dengue cases are reported annually in Bali. Despite the endemicity, limited data on dengue is available for Bali localities. Molecular surveillance study was conducted to explore the clinical and virological characteristics of dengue patients in urban Denpasar and rural Gianyar areas in Bali during the peak season in 2015. A total of 205 adult dengue-suspected patients were recruited in a prospective cross-sectional study. Demographic and clinical information were obtained, and dengue screening was performed using NS1 and IgM/IgG ELISAs...
May 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28518476/adult-onset-chronic-cyclic-thrombocytopenia-in-a-rhesus-macaque-macaca-mulatta-after-dengue-virus-vaccination-and-viral-challenge
#8
Galit H Frydman, Kelly A Metcalf Pate, Robert P Marini, Armelle M de Laforcade, Irene Bosch, Vasudevan Bakthavatchalu, Sureshkumar Muthupalani, Alton G Swennes, Claire E Lyons, Ronald G Tompkins, James G Fox
An 8-year-old, male Rhesus macaque (Macaca mulatta), previously used for dengue virus (DENV) vaccine research with viral challenge, was presented with adult-onset, chronic, cyclic thrombocytopenia. Platelet number, morphology, and function were evaluated by automated hematology, peripheral blood smears, electron microscopy, flow cytometry, and impedance aggregometry. Bone marrow was evaluated by cytology. Both serum anti-dengue nonstructural protein 1 (NS1) antibodies and anti-platelet antibodies were detected by ELISA...
June 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28515302/transposon-mutagenesis-of-the-zika-virus-genome-highlights-regions-essential-for-rna-replication-and-restricted-for-immune-evasion
#9
Benjamin O Fulton, David Sachs, Megan C Schwarz, Peter Palese, Matthew J Evans
The molecular constraints affecting Zika virus (ZIKV) evolution are not well understood. To investigate ZIKV genetic flexibility, we used transposon mutagenesis to add 15-nucleotide insertions throughout the MR766 ZIKV genome and subsequently deep sequenced the viable mutants. Few ZIKV insertion mutants replicated, which likely reflects a high degree of functional constraints on the genome. The NS1 gene exhibited distinct mutational tolerances at different stages of the screen. This result may define regions of the NS1 protein that are required for the different stages of the viral life cycle...
May 17, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28505571/re-evaluation-of-routine-dengue-virus-serology-in-travelers-in-the-era-of-zika-virus-emergence
#10
Maurits P A van Meer, Ramona Mögling, Janienne Klaasse, Felicity D Chandler, Suzan D Pas, Annemiek A van der Eijk, Marion P G Koopmans, Chantal B E M Reusken, Corine H GeurtsvanKessel
BACKGROUND: Diagnostic requests for both Zika virus (ZIKV) and dengue virus (DENV) infections in returning travelers have significantly increased during the recent ZIKV outbreak in the Americás. These flaviviruses have overlapping clinical syndromes and geographical distribution, but diagnostic differentiation is important because of different clinical consequences. As flaviviruses are known to have a short viremic period, diagnostics often rely on serological methods, which are challenging due to extensive cross-reactive antibodies...
May 3, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28491251/significance-of-igg-optical-density-ratios-index-value-in-single-reactive-anti-dengue-virus-igg-capture-elisa
#11
Shiv Sekhar Chatterjee, Ankush Sharma, Shilpee Choudhury, Sushil Kumar Chumber, Mandeep Kaur, Ras Bage, Nittin Parkhe, Uma Khanduri
BACKGROUND AND OBJECTIVES: A single reactive IgG anti-Dengue virus ELISA test in the absence of IgM antibodies or NS1 antigen may denote current infection or past exposure to the virus. To determine whether IgG index value can be used to identify true current dengue infection we conducted a prospective observational study. MATERIALS AND METHODS: Suspected dengue patients (n =1745) were tested in their first specimen by MAC-ELISA, GAC-ELISA and NS1 antigen ELISA...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28480265/clinical-utility-of-the-nonstructural-1-antigen-rapid-diagnostic-test-in-the-management-of-dengue-in-returning-travelers-with-fever
#12
Ralph Huits, Patrick Soentjens, Ula Maniewski-Kelner, Caroline Theunissen, Steven Van Den Broucke, Eric Florence, Jan Clerinx, Erika Vlieghe, Jan Jacobs, Lieselotte Cnops, Dorien Van Den Bossche, Marjan Van Esbroeck, Emmanuel Bottieau
BACKGROUND: Rapid diagnostic test (RDT) detecting the nonstructural 1 (NS1) antigen is increasingly used for dengue diagnosis in endemic and nonendemic settings, but its clinical utility has not been studied in travel clinic practice. METHODS: From August 2012 to July 2016, travelers returning from the tropics with fever were evaluated in the Institute of Tropical Medicine (Antwerp, Belgium) with the routine use of NS1 antigen RDT that provided results within 1 hour...
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28469422/seroprevalence-of-asymptomatic-dengue-virus-infection-and-its-antibodies-among-healthy-eligible-saudi-blood-donors-findings-from-holy-makkah-city
#13
Ahmed M Ashshi, Saad Alghamdi, Adel G El-Shemi, Sabir Almdani, Bassem Refaat, Amr M Mohamed, Hani O Ghazi, Esam I Azhar, Faisal A Al-Allaf
BACKGROUND: Threat to blood transfusion-transmitted dengue virus (DENV) and its antibodies has recently emerged worldwide. Dengue fever is an endemic disease in Saudi Arabia, particularly in its Western region. The aim of this study was to estimate the seroprevalence of asymptomatic DENV infection and its antibodies among eligible Saudi blood donors. METHODS: Serum samples from 910 healthy/eligible adult male Saudi blood donors, who reside in Holy Makkah City of Saudi Arabia, were collected between March 2015 and August 2016 and screened for the detection of DENV nonstructural protein 1 (NS1) antigen and anti-DENV IgM and IgG antibodies using commercial enzyme-linked immunosorbent assay kits (Panbio, Brisbane, QLD, Australia)...
2017: Virology: Research and Treatment
https://www.readbyqxmd.com/read/28465676/neurological-complications-and-death-in-children-with-dengue-virus-infection-report-of-two-cases
#14
Neydi Osnaya-Romero, Maria-Gabriela Perez-Guille, Sandra Andrade-García, Erika Gonzalez-Vargas, Rebeca Borgaro-Payro, Sandra Villagomez-Martinez, José de Jesús Ortega-Maldonado, Jose Luis Arredondo-García
BACKGROUND: Dengue virus infection can have different complications; the best known is hemorrhagic dengue fever. However, other effects such as neurological disorders may endanger the lives of patients. Dengue neurological manifestations can be confused with encephalitis symptoms and can lead to cerebral edema and death. Therefore, we consider important in the endemic areas to take into account the diagnosis of dengue encephalitis in patients with neurological disorders, and to request the determination of serology in cerebrospinal fluid for the NS1 antigen test...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28456663/comparative-whole-genome-analysis-of-dengue-virus-serotype-2-strains-differing-in-trans-endothelial-cell-leakage-induction-in-vitro
#15
Sneha Singh, M G Anupriya, Easwaran Sreekumar
The role of genetic differences among dengue virus (DENV) in causing increased microvascular permeability is less explored. In the present study, we compared two closely related DENV serotype-2 strains of Cosmopolitan genotype for their in vitro infectivity phenotype and ability to induce trans-endothelial leakage. We found that these laboratory strains differed significantly in infecting human microvascular endothelial cells (HMEC-1) and hepatocytes (Huh7), two major target cells of DENV in in vivo infections...
April 27, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28453108/-phylogenetic-analysis-of-south-american-sequences-of-the-nonstructural-protein-1-ns1-of-dengue-serotype-2-associated-with-severe-clinical-bleeding
#16
Raimundo Castro-Orozco, Lyda R Castro-García, Doris E Gómez-Camargo
Objective The objective of this in silico study was to compare nucleotide and amino acids DENV-2-NS1 sequences isolated from febrile patients, with and without disease severity, from different South American countries. Matherials and Methods A bayesian MCMC phylogenetic analysis was carried out using 28 complete sequences of the gene NS1 of the DENV-2 serotype (1 056 bp), using MrBayes v.3.2.0 software, with the model SYM+G (2.5 million generations). We also carried out a phylogenetic analysis with Neighbor-Joining method (Jukes-Cantor model)...
June 2016: Revista de Salud Pública
https://www.readbyqxmd.com/read/28444295/features-of-dengue-and-chikungunya-infections-of-colombian-children-under-24%C3%A2-months-of-age-admitted-to-the-emergency-department
#17
Angel Paternina-Caicedo, Fernando De la Hoz-Restrepo, Fredi Díaz-Quijano, William Caicedo-Torres, María Auxiliadora Badillo-Viloria, Doris Bula-Anichiarico, Nelson Alvis-Guzmán, Salim Mattar, Dagna Constenla, Hernando Pinzón-Redondo
We aimed to assess clinical and laboratory differences between dengue and chikungunya in children <24 months of age in a comparative study. We collected retrospective clinical and laboratory data confirmed by NS1/IgM for dengue for 19 months (1 January 2013 to 17 August 2014). Prospective data for chikungunya confirmed by real-time polymerase chain reaction were collected for 4 months (22 September 2014-14 December 2014). Sensitivity and specificity [with 95% confidence interval (CI)] were reported for each disease diagnosis...
April 21, 2017: Journal of Tropical Pediatrics
https://www.readbyqxmd.com/read/28441781/dengue-virus-non-structural-protein-5
#18
REVIEW
Abbas El Sahili, Julien Lescar
The World Health Organization estimates that the yearly number of dengue cases averages 390 million. This mosquito-borne virus disease is endemic in over 100 countries and will probably continue spreading, given the observed trend in global warming. So far, there is no antiviral drug available against dengue, but a vaccine has been recently marketed. Dengue virus also serves as a prototype for the study of other pathogenic flaviviruses that are emerging, like West Nile virus and Zika virus. Upon viral entry into the host cell and fusion of the viral lipid membrane with the endosomal membrane, the viral RNA is released and expressed as a polyprotein, that is then matured into three structural and seven non-structural (NS) proteins...
April 24, 2017: Viruses
https://www.readbyqxmd.com/read/28437586/pathogenesis-of-vascular-leak-in-dengue-virus-infection
#19
REVIEW
Gathsaurie Neelika Malavige, Graham S Ogg
Endothelial dysfunction leading to vascular leak is the hallmark of severe dengue. Vascular leak typically becomes clinically evident 3-6 days after the onset of illness, which is known as the critical phase. This critical phase follows the period of peak viraemia, and lasts for 24-48 hr and usually shows rapid and complete reversal, suggesting that it is likely to occur as a result of inflammatory mediators, rather than infection of the endothelium. Cytokines such as tumour necrosis factor-α, which are known to be elevated in the critical phase of dengue, are likely to be contributing factors...
July 2017: Immunology
https://www.readbyqxmd.com/read/28400226/in-vitro-effect-of-human-cathelicidin-antimicrobial-peptide-ll-37-on-dengue-virus-type-2
#20
K Alagarasu, P S Patil, P Shil, M Seervi, M B Kakade, H Tillu, A Salunke
Human Cathelicidin antimicrobial peptide LL-37 is known to have antiviral activity against many viruses. In the present study, we investigated the in-vitro effect of LL-37 on dengue virus type 2 (DENV-2) infection and replication in Vero E6 cells. To study the effect of pretreatment of virus or cells with LL-37, the virus was pretreated with different concentrations of LL-37 (2.5μM-15μM) or scrambled (Scr) LL-37(5μM-15μM) and used for infection or the cells were first treated with LL-37 and infected. To study the effect of LL-37 post infection (PI), the cells were infected first followed by addition of LL-37 to the culture medium 24h after infection...
April 9, 2017: Peptides
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