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Dengue NS1

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https://www.readbyqxmd.com/read/29330423/t-cell-immunity-to-zika-virus-targets-immunodominant-epitopes-that-show-cross-reactivity-with-other-flaviviruses
#1
C J Reynolds, O M Suleyman, A M Ortega-Prieto, J K Skelton, P Bonnesoeur, A Blohm, V Carregaro, J S Silva, E A James, B Maillère, M Dorner, R J Boyton, D M Altmann
Zika virus (ZIKV) Infection has several outcomes from asymptomatic exposure to rash, conjunctivitis, Guillain-Barré syndrome or congenital Zika syndrome. Analysis of ZIKV immunity is confounded by the fact that several related Flaviviruses infect humans, including Dengue virus 1-4, West Nile virus and Yellow Fever virus. HLA class II restricted T cell cross-reactivity between ZIKV and other Flaviviruses infection(s) or vaccination may contribute to protection or to enhanced immunopathology. We mapped immunodominant, HLA class II restricted, CD4 epitopes from ZIKV Envelope (Env), and Non-structural (NS) NS1, NS3 and NS5 antigens in HLA class II transgenic mice...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29305550/comparison-of-four-serological-methods-and-two-rt-pcr-assays-for-diagnosis-and-surveillance-of-zika
#2
Angel Balmaseda, Jose Victor Zambrana, Damaris Collado, Nadezna Garcia, Saira Saborío, Douglas Elizondo, Juan Carlos Mercado, Karla Gonzalez, Cristhiam Cerpas, Andrea Nuñez, Davide Corti, Jesse J Waggoner, Guillermina Kuan, Raquel Burger-Calderon, Eva Harris
Zika virus (ZIKV) is a mosquito-borne flavivirus that is responsible for recent explosive epidemics in the Americas. Notably, ZIKV infection during pregnancy has been found to cause congenital birth defects, including microcephaly, and ZIKV has been associated with Guillain Barré Syndrome in adults. Diagnosis and surveillance of Zika in the Americas have been challenging due to similar clinical manifestations and extensive antibody cross-reactivity with endemic flaviviral diseases, like dengue. We evaluated four serological and two RT-PCR methods in acute (mean day 1...
January 5, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29302646/hospital-and-community-based-surveillance-of-zika-and-dengue-viruses-in-indonesia
#3
Nasronudin Suharto, Muhammad Amin, Diah Puspita Rini, Achmad Chusnu Romdhoni, Purwaningsih Zulfayandi Pawanis, Muhammad Syamsul Hidayat
Background: Zika virus remained silent for few decades after its first recognition in 1947. Recently, Zika drew global attention due to the outbreak in Pacific island and Latin Americas. The concern was escalating given the shared vector of Zika and dengue with possible deleterious complications. Surabaya as one of the largest cities in Indonesia with high mobility of people is endemic to dengue. This study was aimed to detect Zika infection during dengue outbreaks in Surabaya. Materials and Methods: This was a surveillance study involving patients visiting 4 public hospitals and 8 primary health care centres (PHCs)...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29302526/epidemiological-characteristics-of-dengue-disease-in-saurashtra-region-india-during-year-2015
#4
Madhulika Mistry, Rajesh K Chudasama, Yogesh Goswami, Chintan Dalwadi, Aarohi Mitra, Garima Mehta
Background: Dengue is an important vector-borne disease with a spectrum of classical fever to hemorrhagic fever to dengue shock syndrome. The present study was conducted with objective to study epidemiological and demographic characteristics of dengue infections during the year 2015 in Saurashtra region, Gujarat state, India. Materials and Methods: The study was conducted at Tertiary Care Hospital, Rajkot, during the year 2015. A total of 3312 blood samples were collected and tested for dengue NS1 antigen and IgM antibody by capture ELISA testing from various districts of Saurashtra region...
April 2017: Journal of Family Medicine and Primary Care
https://www.readbyqxmd.com/read/29300893/virus-and-antibody-dynamics-in-travelers-with-acute-zika-virus-infection
#5
Luisa Barzon, Elena Percivalle, Monia Pacenti, Francesca Rovida, Maurizio Zavattoni, Paola Del Bravo, Anna Maria Cattelan, Giorgio Palù, Fausto Baldanti
Background: To improve our understanding of the natural history of Zika virus (ZIKV) infection in humans, we described the dynamics of ZIKV RNA shedding in different body fluids and antibody responses in patients with acute infection. Methods: Twenty-nine adults with travel-associated infection and 1 case of sexual transmission were enrolled and followed up with weekly ZIKV RNA testing in blood, urine, saliva, and semen samples and antibody testing. Results: ZIKV RNA was detected in plasma, urine, and saliva of 57%, 93...
December 30, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29299882/-about-98-cases-of-dengue-hospitalized-in-a-private-clinic-of-ouagadougou-epidemiology-diagnostic-and-evolution
#6
I Diallo, K A Sondo, H Tieno, E Y Tamelokpo, J Zoungrana, Y Sagna, M Savadogo, A Poda, O Guira, E A Diendéré, J Sakandé, Y J Drabo
Extremely widespread, the dengue is a reemerging infectious disease. In Burkina Faso, dengue is a reality that was little known. The aim of this study was to study epidemiology, diagnostic and outcomes of dengue patients in Ouagadougou. A retrospective study covered a period of two years, from 1 January 2013 to 31 December 2014 in a private clinic in Ouagadougou. Patients included in the study were hospitalized for fever and painful syndrome with a positive test to the dengue non-structural antigen 1 (NS1 Ag)...
December 2017: Bulletin de la Société de Pathologie Exotique
https://www.readbyqxmd.com/read/29291223/ns-1-lasts-longer-than-the-dengue-virus-nucleic-acid-in-the-clinically-suspected-patients-with-dengue-fever-and-dengue-haemorrhagic-fever
#7
Nadeera Sirisena, Faseeha Noordeen, LakKumar Fernando
The objectives of the study were to compare the DENV NS1 and nucleic acid positivity in dengue fever (DF) and dengue haemorrhagic fever (DHF) patients and to evaluate the usefulness of these parameters in severe dengue. Blood samples were collected from 91 patients with DF or DHF (fever days 3-9) from Gampaha and Negombo General Hospitals, Sri Lanka and tested for DENV NS1 antigen and nucleic acid. On fever day 3, 67% of the DF and 33% of the DHF patients had DENV nucleic acid whereas 50% of the DF and 67% of the DHF patients had NS1...
September 2017: Virusdisease
https://www.readbyqxmd.com/read/29282904/human-t-cell-responses-to-dengue-and-zika-virus-infection-compared-to-dengue-zika-coinfection
#8
Jessica Badolato-Corrêa, Juan Camilo Sánchez-Arcila, Thiara Manuele Alves de Souza, Luciana Santos Barbosa, Priscila Conrado Guerra Nunes, Monique da Rocha Queiroz Lima, Mariana Gandini, Ana Maria Bispo de Filippis, Rivaldo Venâncio da Cunha, Elzinandes Leal de Azeredo, Luzia Maria de-Oliveira-Pinto
INTRODUCTION: Zika virus (ZIKV) and dengue virus (DENV) co-circulated during latest outbreaks in Brazil, hence, it is important to evaluate the host cross-reactive immune responses to these viruses. So far, little is known about human T cell responses to ZIKV and no reports detail adaptive immune responses during DENV/ZIKV coinfection. METHODS: Here, we studied T cells responses in well-characterized groups of DENV, ZIKV, or DENV/ZIKV infected patients and DENV-exposed healthy donors...
December 28, 2017: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/29281836/a-global-interactome-map-of-the-dengue-virus-ns1-identifies-virus-restriction-and-dependency-host-factors
#9
Mohamed Lamine Hafirassou, Laurent Meertens, Claudia Umaña-Diaz, Athena Labeau, Ophelie Dejarnac, Lucie Bonnet-Madin, Beate M Kümmerer, Constance Delaugerre, Philippe Roingeard, Pierre-Olivier Vidalain, Ali Amara
Dengue virus (DENV) infections cause the most prevalent mosquito-borne viral disease worldwide, for which no therapies are available. DENV encodes seven non-structural (NS) proteins that co-assemble and recruit poorly characterized host factors to form the DENV replication complex essential for viral infection. Here, we provide a global proteomic analysis of the human host factors that interact with the DENV NS1 protein. Combined with a functional RNAi screen, this study reveals a comprehensive network of host cellular processes involved in DENV infection and identifies DENV host restriction and dependency factors...
December 26, 2017: Cell Reports
https://www.readbyqxmd.com/read/29260850/langmuir-blodgett-nanoassemblies-of-mos2-au-composite-at-the-air-water-interface-for-dengue-detection
#10
Shipra Solanki, Amrita Soni, Manoj K Pandey, Ashok Biradar, Gajjala Sumana
We report Langmuir Blodgett (LB) films of molybdenum disulphide (MoS2) and gold nanoparticles (AuNPs) composite being utilized as biosensing platform for dengue detection. The LB films of MoS2-AuNPs composite have been transferred from air water interface to the indium tin oxide (ITO) coated glass substrate under optimized conditions. Further, antibodies specific to dengue NS1 antigen were immobilized onto these LB films. The fabricated immunosensor has been explored for NS1 antigen detection in standard samples as well as in spiked sera samples using electrochemical impedance spectroscopy...
December 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29247706/episodes-of-the-epidemiological-factors-correlated-with-prevailing-viral-infections-with-dengue-virus-and-molecular-characterization-of-serotype-specific-dengue-virus-circulation-in-eastern-india
#11
M Rajesh Kumar Rao, Rabindra N Padhy, Manoj Kumar Das
BACKGROUND: Dengue is one of the most important and widespread viral infection comprises 4 related serotypes (DEN-1, 2, 3, and 4). Infection with one serotype does not protect against the others, and sequential infections put people at greater risk for dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). This study determines the epidemiology of prevailing viral infections with dengue and molecular characterization of serotype-specific DENV circulation in Odisha of eastern India...
December 13, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29219127/dengue-fever-in-a-municipality-of-west-bengal-india-2015-an-outbreak-investigation
#12
Falguni Debnath, Manickam Ponnaiah, Pralay Acharya
BACKGROUND: In November 2015, death due to fever and increased number of fever cases were reported from Baranagar Municipality of North 24 Parganas district of West Bengal. OBJECTIVES: The episode was investigated with the objective to (1) confirm the existence of an outbreak, (2) describe it in terms of time, place, and person, (3) determine the cause of outbreak, and (4) recommend control measures. METHODS: Monthly incidence of dengue from 2012 to 2014 was calculated and compared with 2015 to confirm the outbreak...
October 2017: Indian Journal of Public Health
https://www.readbyqxmd.com/read/29209371/corrigendum-to-dengue-virus-ns1-protein-as-a-diagnostic-marker-commercially-available-elisa-and-comparison-to-qrt-pcr-and-serological-diagnostic-assays-currently-used-by-the-state-of-florida
#13
Jason H Ambrose, Shamala Devi Sekaran, Azliyati Azizan
[This corrects the article DOI: 10.1155/2017/8072491.].
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29197806/immunofluorescence-based-biosensor-for-the-determination-of-dengue-virus-ns1-in-clinical-samples
#14
Nadiya T Darwish, Shamala D Sekaran, Yatimah Alias, Sook Mei Khor
The sharp increase in incidence of dengue infection has necessitated the development of methods for the rapid diagnosis of this deadly disease. Here we report the design and development of a reliable, sensitive, and specific optical immunosensor for the detection of the dengue nonstructural protein 1 (NS1) biomarker in clinical samples obtained during early stages of infection. The present optical NS1 immunosensor comprises a biosensing surface consisting of specific monoclonal NS1 antibody for immunofluorescence-based NS1 antigen determination using fluorescein isothiocyanate (FITC) conjugated to IgG antibody...
November 30, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29182305/point-of-use-nanobiosensor-for-detection-of-dengue-virus-ns1-antigen-in-adult-aedes-aegypti-a-potential-tool-for-improved-dengue-surveillance
#15
Daniel Aaron Wasik, Ashok Mulchandani, Marylynn V Yates
Dengue virus (DENV) and its primary mosquito vectors Aedes spp., have spread to every continent except Antarctica, causing outbreaks and autochthonous transmission in previously disease-free regions. Recently, the spread of other arboviruses carried by invasive Aedes spp., such as Chikungunya and Zika, seem to be following similar trends as DENV and have renewed interest in monitoring and modelling arboviruses within mosquito vectors. Unfortunately, current commercially available detection methods are designed for the diagnosis of DENV in humans or are too expensive and complex for sustainable monitoring...
November 28, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29176643/development-of-a-quantitative-ns1-capture-enzyme-linked-immunosorbent-assay-for-early-detection-of-yellow-fever-virus-infection
#16
Taissa Ricciardi-Jorge, Juliano Bordignon, Andrea Koishi, Camila Zanluca, Ana Luiza Mosimann, Claudia Nunes Duarte Dos Santos
Yellow fever is an arboviral disease that causes thousands of deaths every year in Africa and the Americas. However, few commercial diagnostic kits are available. Non-structural protein 1 (NS1) is an early marker of several flavivirus infections and is widely used to diagnose dengue virus (DENV) infection. Nonetheless, little is known about the dynamics of Yellow fever virus (YFV) NS1 expression and secretion, to encourage its use in diagnosis. To tackle this issue, we developed a quantitative NS1-capture ELISA specific for YFV using a monoclonal antibody and recombinant NS1 protein...
November 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29167907/definitive-tests-for-dengue-fever-when-and-which-should-i-use
#17
Hui Bin Yvonne Chan, Choon How How, Chung Wai Mark Ng
Dengue is a mosquito-borne viral disease that has established itself globally in both endemic and epidemic transmission cycles. While diagnostic decision-making for dengue is often guided by clinical judgement, definitive laboratory tests, including rapid point-of-care tests, have many advantages in the primary care setting. These include providing epidemiological data and diagnostic clarity for atypical cases, as well as contributing to patient education and compliance. This article discussed the types of diagnostic methods for dengue, when to use them and the appropriateness of each test...
November 2017: Singapore Medical Journal
https://www.readbyqxmd.com/read/29158907/chikungunya-outbreak-in-delhi-india-2016-report-on-coinfection-status-and-comorbid-conditions-in-patients
#18
N Kaur, J Jain, A Kumar, M Narang, M K Zakaria, A Marcello, D Kumar, R Gaind, S Sunil
Chikungunya fever is a major public health issue in India affecting billions. After 2010, the infection was in a decline until in 2016, when a massive outbreak affected the country. In this report, we present serologic and molecular investigations of 600 patient samples for chikungunya and dengue viruses along with clinical and comorbidity features. We recruited 600 patients during this outbreak and evaluated them for chikungunya and dengue virus antibodies and virus RNA through IgM, NS1 antigen and quantitative real-time PCR (qPCR)...
November 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/29141913/multiplexed-biomarker-panels-discriminate-zika-and-dengue-virus-infection-in-humans
#19
Guang Song, Hee-Sool Rho, Jianbo Pan, Pedro Ramos, Ki-Jun Yoon, Freddy A Medina, Emily M Lee, Daniel J Eichinger, Guo-Li Ming, Jorge L Muñoz-Jordan, Hengli Tang, Ignacio Pino, Hongjun Song, Jiang Qian, Heng Zhu
Zika virus (ZIKV) and dengue virus (DENV) are closely related flaviviruses that cause widespread, acute febrile illnesses, notably microcephaly for fetuses of infected pregnant women. Detecting the viral cause of these illnesses is paramount to determine risks to patients, counsel pregnant women, and help fight outbreaks. A combined diagnostic algorithm for ZIKV and DENV requires Reverse transcription polymerase chain reaction (RT-PCR) and IgM antibody detection. RT-PCR differentiates between DENV and ZIKV infections during the acute phases of infection, but differentiation based on IgM antibodies is currently nearly impossible in endemic areas...
November 15, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29140541/regulatory-t-cells-in-acute-dengue-viral-infection
#20
Eresha Jayaratne, Dulharie Wijeratne, Samitha Fernando, Achala Kamaladasa, Laksiri Gomes, Ananda Wijewickrama, Graham Stuart Ogg, Gathsaurie Neelika Malavige
Although regulatory T cells (Tregs) have been shown to be expanded in acute dengue, their role in pathogenesis and their relationship to clinical disease severity, and extent of viraemia have not been fully evaluated. The frequency of Tregs was assessed in 56 adult patients with acute dengue, by determining the proportion of FOXP3 expressing CD4(+) CD25(+) T cells (FOXP3+ cells). DENV viral loads were measured by quantitative real time PCR and DENV specific T cell responses were measured by ex vivo IFNγ ELISpot assays to overlapping peptide pools of DENV-NS3, NS1 and NS5...
November 15, 2017: Immunology
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