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https://www.readbyqxmd.com/read/29149095/proton-nuclear-magnetic-resonance-%C3%A2-h-nmr-based-metabolomic-evaluation-of-human-renal-allografts-from-donations-after-circulatory-death
#1
Zijie Wang, Haiwei Yang, Chunchun Zhao, Jifu Wei, Junsong Wang, Zhijian Han, Jun Tao, Zhen Xu, Xiaobin Ju, Ruoyun Tan, Min Gu
BACKGROUND Delayed graft function (DGF) is a common complication that impairs allograft function after kidney transplantation. However, the mechanism of DGF remains unclear. Nuclear magnetic resonance (NMR)-based analysis has been widely used in recent times to assess changes in metabolite levels. MATERIAL AND METHODS Samples of perfusate from allografts donated after circulatory death were collected prior to transplantation, during static cold storage. ¹H-NMR-based metabolomics combined with the statistical methods, orthogonal partial least-squares discriminant analysis (OPLS-DA), and principle-component analysis (PCA), were employed to test different levels of metabolites between the allografts that exhibited DGF and those that exhibited immediate graft function (IGF)...
November 17, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29147712/a-biocompatible-betaine-functionalized-polycation-for-coacervation
#2
Mintai P Hwang, Xiaochu Ding, Jin Gao, Abhinav P Acharya, Steven R Little, Yadong Wang
The aqueous nature of complex coacervates provides a biologically-relevant context for various therapeutic applications. In this sense, biological applications demand a corresponding level of biocompatibility from the polyelectrolytes that participate in complex coacervation. Continued development with naturally-occurring polyelectrolytes such as heparin and chitosan underscore such aims. Herein, we design a synthetic polycation, in which betaine is conjugated to a biodegradable polyester backbone. Betaine is a naturally-occurring methylated amino acid that is ubiquitously present in human plasma...
November 17, 2017: Soft Matter
https://www.readbyqxmd.com/read/29132024/a-hilic-uhplc-ms-ms-untargeted-urinary-metabonomics-combined-with-quantitative-analysis-of-five-polar-biomarkers-on-osteoporosis-rats-after-oral-administration-of-gushudan
#3
Xiao Wu, Yue Huang, Jinghan Sun, Yongqing Wen, Feng Qin, Longshan Zhao, Zhili Xiong
A HILIC-UHPLC-MS/MS untargeted urinary metabonomic method combined with quantitative analysis of five potential polar biomarkers in rat urine was developed and validated, to further understand the anti-osteoporosis effect of Gushudan(GSD) and its mechanism on prednisolone-induced osteoporosis(OP) rats in this study. The metabolites were separated and identified on Waters BEH HILIC (2.1mm×100mm, 1.7μm) column using the Waters ACQUITY™ ultra performance liquid chromatography system (Waters Corporation, Milford, USA) coupled with a Micromass Quattro Micro™ API mass spectrometer (Waters Corp, Milford, MA, USA)...
October 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29131576/pharmacological-characterization-of-3h-atpca-as-a-substrate-for-studying-the-functional-role-of-the-betaine-gaba-transporter-1-and-the-creatine-transporter
#4
Anas Al-Khawaja, Anne S Haugaard, Ales Marek, Rebekka Löffler, Louise Thiesen, Monica Santiveri, Maria Damgaard, Christoffer Bundgaard, Bente Frølund, Petrine Wellendorph
The betaine/γ-aminobutyric acid (GABA) transporter 1 (BGT1) is one of the four GABA transporters (GATs) involved in the termination of GABAergic neurotransmission. Although suggested to be implicated in seizure management, the exact functional importance of BGT1 in the brain is still elusive. This is partly owing to the lack of potent and selective pharmacological tool compounds that can be used to probe its function. We previously reported the identification of 2-amino-1,4,5,6-tetrahydropyrimidine-5-carboxylic acid (ATPCA), a selective substrate for BGT1 over GAT1/GAT3, but also an agonist for GABAA receptors...
November 13, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29129236/effects-of-betaine-supplementation-on-nitric-oxide-metabolism-atherosclerotic-parameters-and-fatty-liver-in-guinea-pigs-fed-a-high-cholesterol-plus-methionine-diet
#5
Zeynep Küskü-Kiraz, Sema Genc, Seldağ Bekpınar, Yeşim Ünlücerci, Aydın Çevik, Vakur Olgaç, Figen Gürdöl, Müjdat Uysal
OBJECTIVE: The aim of this study was to investigate the effect of high cholesterol (CHOL) and CHOL + methionine (MET) diets on atherogenic and oxidative index parameters and on the factors that influence nitric oxide (NO) bioavailability. Also, attempts were made to determine whether dietary betaine (BET) resulted in any improvement in the changes that occurred after CHOL + MET administration. METHODS: Guinea pigs were fed chow containing 1.5% CHOL with or without 2% MET for 10 wk...
January 2018: Nutrition
https://www.readbyqxmd.com/read/29128745/a-new-method-for-determination-of-cocamidopropyl-betaine-synthesized-from-coconut-oil-through-spectral-shift-of-eriochrome-black-t
#6
Ali Gholami, Mahshid Golestaneh, Zeinab Andalib
Cocamidopropyl betaine (CAPB) is a zwitterionic surfactant that is synthesized using coconut oil and usually supplied in form of an aqueous solution with 25-37% w/w. In this study, a novel method based on UV-visible spectroscopy is developed for an accurate determination of CAPB synthesized from coconut oil. Eriochrome Black T (EBT) as a specific color indicator was added to CAPB and a red shift and color change were observed. This shift leads in increasing wavelength selectivity of the method. The change in the color intensity depends on the concentration of CAPB...
November 9, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29127003/recombinant-alpha-fetoprotein-receptor-binding-domain-co-expression-with-polyglutamate-tags-facilitates-in-vivo-folding-in-e-coli
#7
Murad Mollaev, Neonila Gorokhovets, Elena Nikolskaya, Maria Faustova, Arthur Zabolotsky, Maria Sokol, Oksana Tereshenko, Olga Zhunina, Vitaliy Shvets, Evgeniy Severin, Nikita Yabbarov
A wide range of methods are known to increase the prokaryotic intracellular recombinant proteins solubility, for instance, growth at low temperature, supplementation of culture media with "chemical chaperones" (proline, glycine-betaine, and trehalose), co-expression with chaperones or highly soluble fusion partners. As an alternative, we have introduced the polyglutamate tag, which, as it has been shown, increased the protein solubility and facilitated folding. In this study we evaluated the minimal quantity of high density negatively charged EEEEVE amino acid repeats (pGlu) necessary to switch the recombinant receptor-binding domain of human alpha-fetoprotein (rbdAFP) expression almost entirely from the inclusion bodies to the soluble cytoplasmic fraction in E...
November 7, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/29125973/creation-of-antifouling-microarrays-by-photopolymerization-of-zwitterionic-compounds-for-protein-assay-and-cell-patterning
#8
Xiuhua Sun, Huaixin Wang, Yuanyuan Wang, Taijiang Gui, Ke Wang, Changlu Gao
Nonspecific binding or adsorption of biomolecules presents as a major obstacle to higher sensitivity, specificity and reproducibility in microarray technology. We report herein a method to fabricate antifouling microarray via photopolymerization of biomimetic betaine compounds. In brief, carboxybetaine methacrylate was polymerized as arrays for protein sensing, while sulfobetaine methacrylate was polymerized as background. With the abundant carboxyl groups on array surfaces and zwitterionic polymers on the entire surfaces, this microarray allows biomolecular immobilization and recognition with low nonspecific interactions due to its antifouling property...
November 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29121861/transcriptomics-metabolomics-and-histology-indicate-that-high-carbohydrate-diet-negatively-affects-the-liver-health-of-blunt-snout-bream-megalobrama-amblycephala
#9
Wassana Prisingkorn, Panita Prathomya, Ivan Jakovlić, Han Liu, Yu-Hua Zhao, Wei-Min Wang
BACKGROUND: Global trend of the introduction of high levels of relatively cheap carbohydrates to reduce the amount of costly protein in the aquatic animal feed production has affected the aquaculture of an economically important cyprinid fish, blunt snout bream (Megalobrama amblycephala). This dietary shift has resulted in increased prevalence of metabolic disorders, often causing economic losses. High dietary intake of carbohydrates, associated with obesity, is one of the major causes of non-alcoholic fatty liver disease (NAFLD) in humans...
November 9, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29115477/effects-of-lycium-barbarum-goji-berry-on-dry-eye-disease-in-rats
#10
Kaung-Jen Chien, Chi-Ting Horng, Yu-Syuan Huang, Yi-Hsien Hsieh, Chau-Jong Wang, Jai-Sing Yang, Chi-Cheng Lu, Fu-An Chen
Lycium barbarum (goji berry) has long been used as a food and traditional herbal medicine. This study aimed to investigate the beneficial effect of the goji berry on dry eye disease in rats. Male Sprague‑Dawley rats with induced dry eye disease were randomly assigned to four groups: Vehicle (control), low‑dose goji berry extract [GBE; 250 mg/kg/body weight (bw)], median‑dose GBE (350 mg/kg/bw), and high‑dose GBE (500 mg/kg/bw). Three methods, Schirmer's test, tear break‑up time (BUT) measurement and keratoconjunctival fluorescein staining, were used to evaluate the effect of GBE on symptoms of dry eye disease experienced by the rats...
November 3, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29110476/certain-perfluoroalkyl-and-polyfluoroalkyl-substances-associated-with-aqueous-film-forming-foam-are-widespread-in-canadian-surface-waters
#11
Lisa A D'Agostino, Scott A Mabury
The presence of perfluoroalkyl and polyfluoroalkyl substances (PFASs) commonly associated with aqueous film forming foams (AFFFs) at sites without known AFFF contamination is a largely unexplored area, which may reveal widespread environmental contaminants requiring further investigation. Sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) screening for 23 classes of PFASs, followed by quantitative analysis was used to investigate surface waters from rural, urban, and AFFF-impacted sites in Canada...
November 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29110324/cnta-oxygenase-substrate-profile-comparison-and-oxygen-dependency-of-tma-production-in-providencia-rettgeri
#12
Gints Kalnins, Eduards Sevostjanovs, Dace Hartmane, Solveiga Grinberga, Kaspars Tars
CntA oxygenase is a Rieske 2S-2Fe cluster-containing protein that has been previously described as able to produce trimethylamine (TMA) from carnitine, gamma-butyrobetaine, glycine betaine, and in one case, choline. TMA found in humans is exclusively of bacterial origin, and its metabolite, trimethylamine oxide (TMAO), has been associated with atherosclerosis and heart and renal failure. We isolated four different Rieske oxygenases and determined that there are no significant differences in their substrate panels...
November 7, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/29104571/identification-of-trans-4-hydroxy-l-proline-as-a-compatible-solute-and-its-biosynthesis-and-molecular-characterization-in-halobacillus-halophilus
#13
Kyung Hyun Kim, Baolei Jia, Che Ok Jeon
Halobacillus halophilus, a moderately halophilic bacterium, accumulates a variety of compatible solutes including glycine betaine, glutamate, glutamine, proline, and ectoine to cope with osmotic stress. Non-targeted analysis of intracellular organic compounds using (1)H-NMR showed that a large amount of trans-4-hydroxy-L-proline (Hyp), which has not been reported as a compatible solute in H. halophilus, was accumulated in response to high NaCl salinity, suggesting that Hyp may be an important compatible solute in H...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29101223/taurine-treatment-prevents-derangement-of-the-hepatic-%C3%AE-glutamyl-cycle-and-methylglyoxal-metabolism-in-a-mouse-model-of-classical-homocystinuria-regulatory-crosstalk-between-thiol-and-sulfinic-acid-metabolism
#14
Kenneth N Maclean, Hua Jiang, Stefanos Aivazidis, Eugene Kim, Colin T Shearn, Peter S Harris, Dennis R Petersen, Robert H Allen, Sally P Stabler, James R Roede
Cystathionine β-synthase-deficient homocystinuria (HCU) is a poorly understood, life-threatening inborn error of sulfur metabolism. Analysis of hepatic glutathione (GSH) metabolism in a mouse model of HCU demonstrated significant depletion of cysteine, GSH, and GSH disulfide independent of the block in trans-sulfuration compared with wild-type controls. HCU induced the expression of the catalytic and regulatory subunits of γ-glutamyl ligase, GSH synthase (GS), γ-glutamyl transpeptidase 1, 5-oxoprolinase (OPLAH), and the GSH-dependent methylglyoxal detoxification enzyme, glyoxalase-1...
November 3, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29099709/sulphur-containing-amino-acids-metabolism-in-experimental-hyper-and-hypothyroidism-in-rats
#15
V Nechiporuk, N Zaichko, М Korda, A Melnyk, O Koloshko
Hyper- and hypothyroidism are some of the most common endocrinopathies that cause many metabolic disorders including amino acids metabolism. However, a specific molecular mechanism of thyroid hormones influence on sulphur-containing amino acids metabolism has not been established. The aim of our research was to investigate experimentally the influence of thyroid gland functional state on the main enzymatic systems of sulphur-containing amino acids metabolism in liver and kidneys, the content of homocysteine, cysteine and H2S in blood...
October 2017: Georgian Medical News
https://www.readbyqxmd.com/read/29094215/insulin-resistance-and-glycine-metabolism-in-humans
#16
REVIEW
M Adeva-Andany, G Souto-Adeva, E Ameneiros-Rodríguez, C Fernández-Fernández, C Donapetry-García, A Domínguez-Montero
Plasma glycine level is low in patients with obesity or diabetes and the improvement of insulin resistance increases plasma glycine concentration. In prospective studies, hypoglycinemia at baseline predicts the risk of developing type 2 diabetes and higher serum glycine level is associated with decreased risk of incident type 2 diabetes. Consistently, plasma glycine concentration is lower in the lean offspring of parents with type 2 diabetes compared to healthy subjects. Among patients with type 2 diabetes, hypoglycinemia occurs before clinical manifestations of the disease, but the pathophysiological mechanisms underlying glycine deficit and its potential clinical repercussions are unclear...
November 1, 2017: Amino Acids
https://www.readbyqxmd.com/read/29093768/draft-genome-sequence-of-marinobacterium-rhizophilum-cl-yj9-t-dsm-18822-t-isolated-from-the-rhizosphere-of-the-coastal-tidal-flat-plant-suaeda-japonica
#17
Dong Han Choi, Gwang Ii Jang, Alla Lapidus, Alex Copeland, T B K Reddy, Supratim Mukherjee, Marcel Huntemann, Neha Varghese, Natalia Ivanova, Manoj Pillay, Brian J Tindall, Markus Göker, Tanja Woyke, Hans-Peter Klenk, Nikos C Kyrpides, Byung Cheol Cho
The genus Marinobacterium belongs to the family Alteromonadaceae within the class Gammaproteobacteria and was reported in 1997. Currently the genus Marinobacterium contains 16 species. Marinobacterium rhizophilum CL-YJ9(T) was isolated from sediment associated with the roots of a plant growing in a tidal flat of Youngjong Island, Korea. The genome of the strain CL-YJ9(T) was sequenced through the Genomic Encyclopedia of Type Strains, Phase I: KMG project. Here we report the main features of the draft genome of the strain...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/29092879/eggs-early-in-complementary-feeding-increase-choline-pathway-biomarkers-and-dha-a-randomized-controlled-trial-in-ecuador
#18
Lora L Iannotti, Chessa K Lutter, William F Waters, Carlos Andres Gallegos Riofrío, Carla Malo, Gregory Reinhart, Ana Palacios, Celia Karp, Melissa Chapnick, Katherine Cox, Santiago Aguirre, Luis Narvaez, Fernando López, Rohini Sidhu, Pamela Kell, Xuntian Jiang, Hideji Fujiwara, Daniel S Ory, Rebecca Young, Christine P Stewart
Background: Choline status has been associated with stunting among young children. Findings from this study showed that an egg intervention improved linear growth by a length-for-age z score of 0.63.Objective: We aimed to test the efficacy of eggs introduced early in complementary feeding on plasma concentrations of biomarkers in choline pathways, vitamins B-12 and A, and essential fatty acids.Design: A randomized controlled trial, the Lulun ("egg" in Kichwa) Project, was conducted in a rural indigenous population of Ecuador...
November 1, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29090273/nmr-spectra-of-pb2-627-the-rna-binding-domain-in-influenza-a-virus-rna-polymerase-that-contains-the-pathogenicity-factor-lysine-627-and-improvement-of-the-spectra-by-small-osmolytes
#19
Yusuke S Kato, Masaru Tanokura, Takashi Kuzuhara
The influenza A virus, which has an RNA genome, requires RNA-dependent RNA polymerase for transcription and replication. The polymerase is comprised of the subunits PA, PB1, and PB2. The C-terminal RNA-binding domain in PB2 contains lysine 627 (PB2 627), which is associated with pathogenicity and host range. However, the structure and molecular mechanism of PB2 627 in solution remain obscure. Here, we investigated PB2 627 in solution by nuclear magnetic resonance (NMR) and detected inhomogeneity in the intensities of backbone amide proton signals due to local fluctuations in structure...
December 2017: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/29080862/plasma-metabolites-from-choline-pathway-and-risk-of-cardiovascular-disease-in-the-predimed-prevention-with-mediterranean-diet-study
#20
Marta Guasch-Ferré, Frank B Hu, Miguel Ruiz-Canela, Mònica Bulló, Estefanía Toledo, Dong D Wang, Dolores Corella, Enrique Gómez-Gracia, Miquel Fiol, Ramon Estruch, José Lapetra, Montserrat Fitó, Fernando Arós, Lluís Serra-Majem, Emilio Ros, Courtney Dennis, Liming Liang, Clary B Clish, Miguel A Martínez-González, Jordi Salas-Salvadó
BACKGROUND: The relationship between plasma concentrations of betaine and choline metabolism and major cardiovascular disease (CVD) end points remains unclear. We have evaluated the association between metabolites from the choline pathway and risk of incident CVD and the potential modifying effect of Mediterranean diet interventions. METHODS AND RESULTS: We designed a case-cohort study nested within the PREDIMED (Prevention With Mediterranean Diet) trial, including 229 incident CVD cases and 751 randomly selected participants at baseline, followed up for 4...
October 28, 2017: Journal of the American Heart Association
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