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https://www.readbyqxmd.com/read/27910874/comprehensive-evaluation-of-molecular-enhancers-of-the-isothermal-exponential-amplification-reaction
#1
Ellie Mok, Eugene Wee, Yuling Wang, Matt Trau
The exponential amplification reaction (EXPAR) is an emerging isothermal nucleic acid amplification method with high potential for molecular diagnostics due to its isothermal nature and high amplification efficiency. However, the use of EXPAR is limited by the high levels of non-specific amplification. Hence, methods that can improve the specificity of EXPAR are desired to facilitate its widespread adoption in practice. Herein, we proposed a strategy to improve EXPAR performance by using molecular enhancers...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27908547/high-dietary-choline-and-betaine-intake-is-associated-with-low-insulin-resistance-in-the-newfoundland-population
#2
Xiang Gao, Yongbo Wang, Guang Sun
OBJECTIVE: Dietary betaine supplement could ameliorate insulin resistance (IR) in animals, but no data are available for choline. Reports on humans are rare. The aim of this study was to investigate the association between dietary choline and betaine intake and IR in humans. METHODS: We assessed 2394 adults from the CODING (Complex Diseases in the Newfoundland population: Environment and Genetics) study. Intake of dietary choline and betaine was evaluated from the Willett Food Frequency Questionnaire...
January 2017: Nutrition
https://www.readbyqxmd.com/read/27905001/guidelines-for-diagnosis-and-management-of-the-cobalamin-related-remethylation-disorders-cblc-cbld-cble-cblf-cblg-cblj-and-mthfr-deficiency
#3
Martina Huemer, Daria Diodato, Bernd Schwahn, Manuel Schiff, Anabela Bandeira, Jean-Francois Benoist, Alberto Burlina, Roberto Cerone, Maria L Couce, Angeles Garcia-Cazorla, Giancarlo la Marca, Elisabetta Pasquini, Laura Vilarinho, James D Weisfeld-Adams, Viktor Kožich, Henk Blom, Matthias R Baumgartner, Carlo Dionisi-Vici
BACKGROUND: Remethylation defects are rare inherited disorders in which impaired remethylation of homocysteine to methionine leads to accumulation of homocysteine and perturbation of numerous methylation reactions. OBJECTIVE: To summarise clinical and biochemical characteristics of these severe disorders and to provide guidelines on diagnosis and management. DATA SOURCES: Review, evaluation and discussion of the medical literature (Medline, Cochrane databases) by a panel of experts on these rare diseases following the GRADE approach...
November 30, 2016: Journal of Inherited Metabolic Disease
https://www.readbyqxmd.com/read/27902449/is-n-n-dimethylglycine-n-oxide-a-choline-and-betaine-metabolite
#4
Michael Lever, Christopher J McEntyre, Peter M George, Stephen T Chambers
Choline metabolism is by oxidation to betaine, which is demethylated to N,N-dimethylglycine; dimethylglycine is oxidatively demethylated to sarcosine. This pathway is important for osmoregulation and as a source of methyl groups. We asked whether another metabolite was involved. We synthesized the N-oxide of dimethylglycine (DMGO) by oxidizing dimethylglycine with peracetic acid, and measured DMGO in human plasma and urine by HPLC-MS/MS with positive ion detection, using two chromatography procedures, based on ion exchange and HILIC separations...
November 30, 2016: Biological Chemistry
https://www.readbyqxmd.com/read/27901646/the-time-dependent-health-and-biochemical-effects-in-rats-exposed-to-stainless-steel-welding-dust-and-its-soluble-form
#5
Tadeusz Halatek, Magdalena Stanislawska, Irena Kaminska, Malgorzata Cieslak, Radoslaw Swiercz, Wojciech Wasowicz
Welding processes that generate fumes containing toxic metals, such as hexavalent chromium (Cr(VI)), manganese (Mn), and nickel (Ni), have been implicated in lung injury, inflammation, and lung tumor promotion in animal models. The principal objective of this study was to determine the dynamics of toxic effects of inhalation exposure to morphologically rated welding dust from stainless steel welding and its soluble form in TSE System with a dynamic airflow. We assessed the pulmonary toxicity of welding dust in Wistar rats exposed to 60...
November 30, 2016: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/27896396/metabolic-profiling-of-ob-ob-mouse-fatty-liver-using-hr-mas-1-h-nmr-combined-with-gene-expression-analysis-reveals-alterations-in-betaine-metabolism-and-the-transsulfuration-pathway
#6
Mikheil Gogiashvili, Karolina Edlund, Kathrin Gianmoena, Rosemarie Marchan, Alexander Brik, Jan T Andersson, Jörg Lambert, Katrin Madjar, Birte Hellwig, Jörg Rahnenführer, Jan G Hengstler, Roland Hergenröder, Cristina Cadenas
Metabolic perturbations resulting from excessive hepatic fat accumulation are poorly understood. Thus, in this study, leptin-deficient ob/ob mice, a mouse model of fatty liver disease, were used to investigate metabolic alterations in more detail. Metabolites were quantified in intact liver tissues of ob/ob (n = 8) and control (n = 8) mice using high-resolution magic angle spinning (HR-MAS) (1)H-NMR. In addition, after demonstrating that HR-MAS (1)H-NMR does not affect RNA integrity, transcriptional changes were measured by quantitative real-time PCR on RNA extracted from the same specimens after HR-MAS (1)H-NMR measurements...
November 28, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27891771/simultaneous-determination-of-trimethylamine-and-trimethylamine-n-oxide-in-mouse-plasma-samples-by-hydrophilic-interaction-liquid-chromatography-coupled-to-tandem-mass-spectrometry
#7
Si Mi, Yuan-Yuan Zhao, René L Jacobs, Jonathan M Curtis
A method was developed that applies hydrophilic interaction liquid chromatography with tandem mass spectrometry in the multiple-reaction monitoring mode to separate and accurately quantify trimethylamine and trimethylamine N-oxide in a single chromatographic run. This was achieved by converting trimethylamine to ethyl betaine, which is less volatile and hence results in greatly improved quantitation. Ethyl betaine also gives a similar response to trimethylamine N-oxide using positive ion electrospray ionization mass spectrometry...
November 28, 2016: Journal of Separation Science
https://www.readbyqxmd.com/read/27890800/metabolic-responses-of-haliotis-diversicolor-to-vibrio-parahaemolyticus-infection
#8
Jie Lu, Yanyan Shi, Shuhui Cai, Jianghua Feng
Vibrio parahemolyticus is a devastating bacterial pathogen that often causes outbreak of vibriosis in abalone Haliotis diversicolor. Elucidation of metabolic mechanisms of abalones in responding to V. parahemolyticus infection is essential for controlling the epidemic. In this work, (1)H NMR-based metabolomic techniques along with correlation and network analyses are used to investigate characteristic metabolites, as well as corresponding disturbed pathways in hepatopancreas and gill of H. diversicolor after V...
November 24, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27888993/identifying-biomarkers-for-asthma-diagnosis-using-targeted-metabolomics-approaches
#9
William Checkley, Maria P Deza, Jost Klawitter, Karina M Romero, Jelena Klawitter, Suzanne L Pollard, Robert A Wise, Uwe Christians, Nadia N Hansel
BACKGROUND: The diagnosis of asthma in children is challenging and relies on a combination of clinical factors and biomarkers including methacholine challenge, lung function, bronchodilator responsiveness, and presence of airway inflammation. No single test is diagnostic. We sought to identify a pattern of inflammatory biomarkers that was unique to asthma using a targeted metabolomics approach combined with data science methods. METHODS: We conducted a nested case-control study of 100 children living in a peri-urban community in Lima, Peru...
December 2016: Respiratory Medicine
https://www.readbyqxmd.com/read/27886045/nutrition-one-carbon-metabolism-and-neural-tube-defects-a-review
#10
REVIEW
Kelei Li, Mark L Wahlqvist, Duo Li
Neural tube defects (NTDs) are a group of severe congenital malformations, induced by the combined effects of genes and the environment. The most valuable finding so far has been the protective effect of folic acid supplementation against NTDs. However, many women do not take folic acid supplements until they are pregnant, which is too late to prevent NTDs effectively. Long-term intake of folic acid-fortified food is a good choice to solve this problem, and mandatory folic acid fortification should be further promoted, especially in Europe, Asia and Africa...
November 23, 2016: Nutrients
https://www.readbyqxmd.com/read/27881594/hepatic-activity-and-transcription-of-betaine-homocysteine-methyltransferase-methionine-synthase-and-cystathionine-synthase-in-periparturient-dairy-cows-are-altered-to-different-extents-by-supply-of-methionine-and-choline
#11
Zheng Zhou, Timothy A Garrow, Xianwen Dong, Daniel N Luchini, Juan J Loor
BACKGROUND: Compared with choline, Met enhances milk yield and feed intake, and elicits a better immuno-metabolic status in periparturient cows. It is unknown whether hepatic activity and transcription of betaine-homocysteine methyltransferase (BHMT), 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), and cystathionine β-synthase (CBS) are responsive to Met and choline supply. OBJECTIVE: This study sought to characterize hepatic BHMT, MTR, and CBS transcription and activity in response to Met and choline supplementation...
November 23, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27881338/-plasma-metabonomics-of-guifu-dihuang-wan-in-the-treatment-of-yang-deficiency
#12
Ya Xiao, Yuan Jing, Jie-Yu Chen, Fei Li, Jing-Ru Cheng, Jian-Lu Bi, Ren Luo, Xiao-Shan Zhao
OBJECTIVE: To assess the effect of Guifu Dihuang Wan (GFDHW) in the treatment of yang deficiency and explore the underlying molecular mechanism. METHODS: Sixty-two participants without diseases were randomized into control group (n=31) and experimental group (n=31) and were given lifestyle intervention additional GFDHW treatment for a month. NMR technology was used for metabonomics analysis. RESULTS: Intervention with GFDHW resulted in significantly decreased conversion scores of yang deficiency in the experimental group compared with the control group (P<0...
November 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27879948/efficacy-and-local-tolerability-of-different-spray-products-in-the-treatment-of-mild-to-moderate-acne-of-the-back-and-chest-a-controlled-3-arm-assessor-blinded-prospective-trial
#13
Ada Lo Schiavo, Rosa V Puca, Francesca Romano, Massimo Milani
BACKGROUND: A spray formulation containing 2 vitamin-A derivatives (hydroxypinacolone retinoate and retinol) carried in glycospheres (RetinSphere®) combined with an antimicrobial peptide (BIOPEP-15), salicylic acid and vitamin E (BR) has been recently developed for the treatment of truncal acne. We evaluated clinical efficacy of BR in comparison with two commonly used sprays, containing triethylcitrate and ethylinoleate (AK) and containing betaine, glycine and salicylic acid 2% (SP)...
November 23, 2016: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
https://www.readbyqxmd.com/read/27876555/one-carbon-metabolism-and-epigenetics
#14
REVIEW
Simonetta Friso, Silvia Udali, Domenica De Santis, Sang-Woon Choi
The function of one-carbon metabolism is that of regulating the provision of methyl groups for biological methylation reactions including that of DNA and histone proteins. Methylation at specific sites into the DNA sequence and at histone tails are among the major epigenetic feature of mammalian genome for the regulation of gene expression. The enzymes within one-carbon metabolism are dependent from a number of vitamins or nutrients that serve either as co-factors or methyl acceptors or donors among which folate, vitamin B12, vitamin B6, betaine, choline and methionine have a major role...
November 19, 2016: Molecular Aspects of Medicine
https://www.readbyqxmd.com/read/27874036/natural-zwitterionic-betaine-enables-cells-to-survive-ultrarapid-cryopreservation
#15
Jing Yang, Nana Cai, Hongwen Zhai, Jiamin Zhang, Yingnan Zhu, Lei Zhang
Cryoprotectants (CPAs) play a critical role in cryopreservation because they can resist the cell damage caused by the freezing process. Current state-of-the-art CPAs are mainly based on an organic solvent dimethyl sulfoxide (DMSO), and several DMSO-cryopreserved cell products have been brought to market. However, the intrinsic toxicity and complex freezing protocol of DMSO still remain as the bottleneck of the wide use for clinical applications. Herein, we reported that betaine, a natural zwitterionic molecule, could serve as a nontoxic and high efficient CPA...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27864387/increased-trimethylamine-n-oxide-portends-high-mortality-risk-independent-of-glycemic-control-in-patients-with-type-2-diabetes-mellitus
#16
W H Wilson Tang, Zeneng Wang, Xinmin S Li, Yiying Fan, Daniel S Li, Yuping Wu, Stanley L Hazen
BACKGROUND: Recent studies show a mechanistic link between intestinal microbial metabolism of dietary phosphatidylcholine and coronary artery disease pathogenesis. Concentrations of a proatherogenic gut microbe-generated metabolite, trimethylamine N-oxide (TMAO), predict increased incident cardiovascular disease risks in multiple cohorts. TMAO concentrations are increased in patients with type 2 diabetes mellitus (T2DM), but their prognostic value and relation to glycemic control are unclear...
November 18, 2016: Clinical Chemistry
https://www.readbyqxmd.com/read/27861990/contact-sensitization-in-dutch-children-and-adolescents-with-and-without-atopic-dermatitis%C3%A2-%C3%A2-a-retrospective-analysis
#17
Stefanie Lubbes, Thomas Rustemeyer, Johannes H Sillevis Smitt, Marie-Louise A Schuttelaar, Maritza A Middelkamp-Hup
BACKGROUND: Allergic contact dermatitis is known to occur in children with and without atopic dermatitis, but more data are needed on contact sensitization profiles in these two groups. OBJECTIVES: To identify frequent allergens in children with and without atopic dermatitis suspected of having allergic contact dermatitis. METHODS: A retrospective analysis of children aged 0-17 years patch tested between 1996 and 2013 was performed. RESULTS: Of all 1012 children tested because of suspected contact dermatitis, 46% developed one or more positive reactions, the proportions for children with (n = 526) and without (n = 395) atopic dermatitis being 48% and 47%, respectively...
November 11, 2016: Contact Dermatitis
https://www.readbyqxmd.com/read/27856995/choline-protects-against-intestinal-failure-associated-liver-disease-in-parenteral-nutrition-fed-immature-rats
#18
Jie Zhu, Ting Lu, Fei Chen, Junkai Yan, Fan Chen, Qiaosen Zhang, Jifan Wang, Weihui Yan, Tingxi Yu, Qingya Tang, Wei Cai
BACKGROUND: Deficiency of choline, a required nutrient, is related to intestinal failure-associated liver disease (IFALD). Therefore, we aimed to investigate the effects of choline supplementation on IFALD and the underlying mechanisms. METHODS: Male Sprague-Dawley rats (4 weeks old) were fed AIN-93G chow and administered intravenous 0.9% saline (control), parenteral nutrition (PN), or PN plus intravenous choline (600 mg/kg) for 7 days. We evaluated body weight, hepatic histology, biochemical indicators, triglycerides, oxidative status, methylation levels of peroxisomal proliferator-activated receptor alpha (PPARα) gene promoter, expression of PPARα and carnitine palmitoyltransferase 1 (CPT1), and levels of choline metabolites...
November 17, 2016: JPEN. Journal of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/27855528/dietary-anthropometric-and-biochemical-factors-influencing-plasma-choline-carnitine-trimethylamine-and-trimethylamine-n-oxide-concentrations
#19
Anna M Malinowska, Artur Szwengiel, Agata Chmurzynska
The objective of the study was to evaluate the nutritional, anthropometric, and biochemical factors that influence choline, l-carnitine, trimethylamine (TMA), and trimethylamine-N-oxide (TMAO) metabolism in elderly women. The volunteers' diet was assessed using a food frequency questionnaire. Dietary patterns were estimated using a self-established score method. Body mass index (BMI), serum glucose, total, HDL, LDL cholesterol, triacylglycerol, homocysteine (tHcy), free choline (fchol), L-carnitine, TMA, and TMAO were assessed...
November 17, 2016: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/27849316/protective-effect-of-betaine-against-burn-induced-pulmonary-injury-in-rats
#20
Ahmet Özer Şehirli, Burcu Satılmış, Şermin Tetik, Şule Çetinel, Berrak Yeğen, Aslı Aykaç, Göksel Şener
BACKGROUND: This study was designed to determine possible protective effect of betaine treatment against oxidative injury in pulmonary tissue induced with thermal trauma. METHODS: Under ether anesthesia, shaved dorsum of Wistar albino rats was exposed to a 90°C water bath for 10 seconds to induce burn injury. Betaine was administered orally (250 mg/kg) for a period of 21 days before burn injury, and single dose of betaine was administered after thermal injury. Control group rats were exposed to 25°C water bath for 10 seconds...
September 2016: Ulusal Travma Ve Acil Cerrahi Dergisi, Turkish Journal of Trauma & Emergency Surgery: TJTES
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