keyword
MENU ▼
Read by QxMD icon Read
search

Follow

keyword
https://www.readbyqxmd.com/read/29224250/multiple-autologous-bone-marrow-derived-cd271-mesenchymal-stem-cell-transplantation-overcomes-drug-resistant-epilepsy-in-children
#1
Olga Milczarek, Danuta Jarocha, Anna Starowicz-Filip, Stanislaw Kwiatkowski, Bogna Badyra, Marcin Majka
There is a need among patients suffering from drug-resistant epilepsy (DRE) for more efficient and less toxic treatments. The objective of the present study was to assess the safety, feasibility, and potential efficacy of autologous bone marrow cell transplantation in pediatric patients with DRE. Two females and two males (11 months to 6 years) were enrolled and underwent a combined therapy consisting of autologous bone marrow nucleated cells (BMNCs) transplantation (intrathecal: 0.5 × 109 ; intravenous: 0...
December 10, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29224236/prognostic-factors-of-prostate-cancer-mortality-in-a-finnish-randomized-screening-trial
#2
Subas Neupane, Ewout Steyerberg, Jani Raitanen, Kirsi Talala, Juho Pylväläinen, Kimmo Taari, Teuvo Lj Tammela, Anssi Auvinen
OBJECTIVES: To identify the prognostic factors of prostate cancer death among patients enrolled in a Finnish prostate cancer screening trial. METHODS: Data on TNM stage, Gleason score, serum prostate-specific antigen at diagnosis, comorbidity and primary treatment were collected from medical records, as well as date and cause of death from Statistics Finland. Four prognostic risk groups were defined based on TNM stage, Gleason score and prostate-specific antigen at diagnosis...
December 10, 2017: International Journal of Urology: Official Journal of the Japanese Urological Association
https://www.readbyqxmd.com/read/29224233/review-article-methodology-for-the-rapid-review-series-on-musculoskeletal-injuries-in-the-emergency-department
#3
REVIEW
Kirsten Strudwick, Megan McPhee, Anthony Bell, Melinda Martin-Khan, Trevor Russell
Musculoskeletal injuries are a common presentation to the ED, with significant costs involved in the management of these injuries, variances in care within the ED and associated morbidity. A series of rapid review papers were completed to guide best practice for the assessment and management of common musculoskeletal injuries presenting to the ED. This paper presents the methodology used across the rapid reviews. PubMed, CINAHL, EMBASE, TRIP and the grey literature, including relevant organisational websites, were searched in 2015...
December 10, 2017: Emergency Medicine Australasia: EMA
https://www.readbyqxmd.com/read/29224223/altering-the-school-breakfast-environment-reduces-barriers-to-school-breakfast-participation-among-diverse-rural-youth
#4
Mary O Hearst, Amy Shanafelt, Qi Wang, Robert Leduc, Marilyn S Nanney
BACKGROUND: The purpose is to determine if altering school breakfast policies and the school breakfast environment will positively impact adolescent beliefs of the barriers and benefits of eating breakfast. METHODS: There were 904 adolescents from 16 rural high schools, Minnesota, in the BreakFAST Study who reported eating breakfast fewer than 4 times per week at baseline. Schools were randomized to intervention (N = 8 schools) or delayed intervention (N = 8) condition...
January 2018: Journal of School Health
https://www.readbyqxmd.com/read/29224219/measuring-school-functioning-in-students-with-chronic-fatigue-syndrome-a-systematic-review
#5
Michelle Tollit, Jennifer Politis, Sarah Knight
BACKGROUND: It is often surmised that school functioning is significantly impacted in chronic fatigue syndrome (CFS); however, how this phenomenon manifests itself has rarely been characterized. METHODS: This systematic review synthesized and critically appraised methods, constructs, and instruments used to assess school functioning in students with CFS. Searches were conducted in electronic databases (CINAHL, MEDLINE, PubMed, ERIC, and PsycINFO) to locate empirical studies that measured school functioning in children and adolescents with CFS...
January 2018: Journal of School Health
https://www.readbyqxmd.com/read/29224195/prevalence-of-soft-tissue-calcifications-in-the-maxillofacial-region-detected-by-cone-beam-ct
#6
Eucaé Miranda Missias, Eduarda Helena Leandro Nascimento, Maria Luiza Dos Anjos Pontual, Andréa Dos Anjos Pontual, Deborah Queiroz Freitas, Danyel Elias da Cruz Perez, Flávia Maria de Moraes Ramos-Perez
OBJECTIVE: To determine the prevalence of soft tissue calcifications in cone beam computed tomography (CBCT) scans with different fields of view (FOV), and to assess its relation with the clinical relevance of the CBCT findings. MATERIAL AND METHODS: One thousand CBCT scans were grouped according to FOV's size and region scanned (MX-FOV, maxilla; MD-FOV, mandible; and MM-FOV, maxilla/mandible) and evaluated for the presence of soft tissue calcifications. Laterality and length of the elongated styloid processes (ESP) were also assessed...
December 10, 2017: Oral Diseases
https://www.readbyqxmd.com/read/29224191/bilateral-choroiditis-as-the-only-sign-of-persistent-mycobacterium-intracellulare-infection-following-haematogenous-spread-in-an-immunocompromised-patient
#7
Alessandro Invernizzi, Davide Ricaboni, Marco Franzetti, Giovanni Staurenghi, Peter McCluskey, Fabio Franzetti
An immunocompromised patient had positive blood cultures for Mycobacterium intracellulare and no identifiable organ seeding was started on treatment. One month later, the patient was clinically well with negative blood cultures but drug-induced myelotoxicity had developed. Ocular fundus examination at this time revealed bilateral choroidal granulomas which changed patient management.
December 9, 2017: Infection
https://www.readbyqxmd.com/read/29224190/metabolic-surgery-for-the-treatment-of-type-2-diabetes-in-obese-individuals
#8
REVIEW
David E Cummings, Francesco Rubino
Several bariatric operations originally designed to promote weight loss have been found to powerfully treat type 2 diabetes, causing remission in most cases, through diverse mechanisms additional to the secondary consequences of weight loss. These observations have prompted consideration of such operations as 'metabolic surgery', used expressly to treat diabetes, including among patients who are only mildly obese or merely overweight. Large, long-term observational studies consistently demonstrate that bariatric/metabolic surgery is associated with reductions in all cardiovascular risk factors, actual cardiovascular events, microvascular diabetes complications, cancer and death...
December 9, 2017: Diabetologia
https://www.readbyqxmd.com/read/29224188/an-evaluation-of-a-social-skills-intervention-for-adults-with-autism-spectrum-disorder-and-intellectual-disabilities-preparing-for-employment-in-ireland-a-pilot-study
#9
Edith Walsh, Jennifer Holloway, Helena Lydon
Individuals with autism spectrum disorder (ASD) are faced with significant barriers relating to employment opportunities and workplace participation. This study evaluated the effectiveness of the Walker social skills curriculum: the ACCESS program and video modeling to increase social communication skills necessary for workplace inclusion. Participants attended two sessions (i.e., 3 h) per week across a period of 20 weeks. A multiple-probe design was used to demonstrate social skills outcomes across three broad curricular areas (i...
December 9, 2017: Journal of Autism and Developmental Disorders
https://www.readbyqxmd.com/read/29224164/hairpin-bisulfite-sequencing-synchronous-methylation-analysis-on-complementary-dna-strands-of-individual-chromosomes
#10
Pascal Giehr, Jörn Walter
The accurate and quantitative detection of 5-methylcytosine is of great importance in the field of epigenetics. The method of choice is usually bisulfite sequencing because of the high resolution and the possibility to combine it with next generation sequencing. Nevertheless, also this method has its limitations. Following the bisulfite treatment DNA strands are no longer complementary such that in a subsequent PCR amplification the DNA methylation patterns information of only one of the two DNA strand is preserved...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224153/multiplexed-dna-methylation-analysis-of-target-regions-using-microfluidics-fluidigm
#11
Martyna Adamowicz, Klio Maratou, Timothy J Aitman
Whole genome shotgun bisulfite sequencing is a method used to generate genome-wide methylation profiles. There are many available protocols to validate the results of this genome-wide method, but they mostly share the limitation of measuring methylation at a small number of CpG positions in small numbers of samples. We developed a multiplexed DNA methylation analysis protocol, which allows for the simultaneous quantitative measurement of cytosine methylation at single nucleotide resolution in 48 PCR amplicons and 48 samples utilizing the microfluidic system established by Fluidigm...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224152/microdroplet-pcr-for-highly-multiplexed-targeted-bisulfite-sequencing
#12
H Kiyomi Komori, Sarah A LaMere, Traver Hart, Steven R Head, Ali Torkamani, Daniel R Salomon
Many methods exist for examining CpG DNA methylation. However, many of these are qualitative, laborious to apply to a large number of genes simultaneously, or are not easy to target to specific regions of interest. Microdroplet PCR-based bisulfite sequencing allows for quantitative single base resolution analysis of investigator selected regions of interest. Following bisulfite conversion of genomic DNA, targeted microdroplet PCR is conducted with custom primer libraries. Samples are then fragmented, concatenated, and sequenced by high-throughput sequencing...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224147/comprehensive-whole-dna-methylome-analysis-by-integrating-medip-seq-and-mre-seq
#13
Xiaoyun Xing, Bo Zhang, Daofeng Li, Ting Wang
Understanding the role of DNA methylation often requires accurate assessment and comparison of these modifications in a genome-wide fashion. Sequencing-based DNA methylation profiling provides an unprecedented opportunity to map and compare complete DNA CpG methylomes. These include whole genome bisulfite sequencing (WGBS), Reduced-Representation Bisulfite-Sequencing (RRBS), and enrichment-based methods such as MeDIP-seq, MBD-seq, and MRE-seq. An investigator needs a method that is flexible with the quantity of input DNA, provides the appropriate balance among genomic CpG coverage, resolution, quantitative accuracy, and cost, and comes with robust bioinformatics software for analyzing the data...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224143/multiplexed-reduced-representation-bisulfite-sequencing-with-magnetic-bead-fragment-size-selection
#14
William P Accomando, Karin B Michels
Reduced representation bisulfite sequencing (RRBS) is a technique for assessing genome-wide DNA methylation in an organism whose genome has been fully sequenced. It allows researchers to target gene regions with particular CpG densities, thereby selecting the desired genomic contexts. Here, we describe an approach that uses magnetic beads to accomplish this selection. In addition, the use of indexed, methylated adapters enables up to 12 samples to be pooled, and subjected to multiplexed RRBS in a single-sequencing lane...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224136/a-summary-of-the-biological-processes-disease-associated-changes-and-clinical-applications-of-dna-methylation
#15
Gitte Brinch Andersen, Jörg Tost
DNA methylation at cytosines followed by guanines, CpGs, forms one of the multiple layers of epigenetic mechanisms controlling and modulating gene expression through chromatin structure. It closely interacts with histone modifications and chromatin remodeling complexes to form the local genomic and higher-order chromatin landscape. DNA methylation is essential for proper mammalian development, crucial for imprinting and plays a role in maintaining genomic stability. DNA methylation patterns are susceptible to change in response to environmental stimuli such as diet or toxins, whereby the epigenome seems to be most vulnerable during early life...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224135/serum-ferritin-and-obstructive-sleep-apnea-epidemiological-study
#16
Elin H Thorarinsdottir, Erna S Arnardottir, Bryndis Benediktsdottir, Christer Janson, Isleifur Olafsson, Allan I Pack, Thorarinn Gislason, Brendan T Keenan
PURPOSE: Ferritin is an intracellular iron storage protein and a marker of inflammation. Studies have shown that subjects with obstructive sleep apnea (OSA) have higher levels of circulating pro-inflammatory cytokines, but little is known about the association between ferritin and OSA. The aims of the study were to evaluate serum ferritin (S-Ferritin) levels in OSA patients compared to levels in the general population and also examine the effect of obesity level and treatment with positive airway pressure (PAP) on S-Ferritin levels...
December 9, 2017: Sleep & Breathing, Schlaf & Atmung
https://www.readbyqxmd.com/read/29224132/treatment-of-mild-to-moderate-hypoglycemia-in-patients-with-type-1-diabetes-treated-with-insulin-pump-therapy-are-current-recommendations-effective
#17
Véronique Gingras, Katherine Desjardins, Mohamed Raef Smaoui, Valérie Savard, Virginie Messier, Ahmad Haidar, Laurent Legault, Rémi Rabasa-Lhoret
AIMS: Mild-to-moderate hypoglycemia (blood glucose < 4.0 mmol/L) is recommended to be treated with 15 g of carbohydrates and to repeat the treatment if hypoglycemia persists after 15 min. This recommendation was established before intensive insulin therapy and based on studies using insulin with different pharmacokinetic profiles from actual insulin analogs showing that 15 g of glucose increases blood glucose by ~ 1.5 mmol/L in 15 min. We aimed to explore the effect of current recommended treatment of mild-to-moderate hypoglycemia in type 1 diabetes (T1D) participants and factors associated with treatment effectiveness...
December 9, 2017: Acta Diabetologica
https://www.readbyqxmd.com/read/29224118/testing-the-hypothesis-of-hierarchical-predictability-in-ecological-restoration-and-succession
#18
Scott R Abella, Timothy A Schetter, Timothy L Walters
To advance predictive ecology, the hypothesis of hierarchical predictability proposes that community measures for which species are interchangeable (e.g., structure and species richness) are more predictable than measures for which species identity matters (e.g., community composition). Predictability is hypothesized to decrease for response measures in order of the following categories: structure, species richness, function, and species composition. We tested this hypothesis using a 14-year, oak savanna-prairie restoration experiment that removed non-native pine plantations at 24 sites in northwestern Ohio, USA...
December 9, 2017: Oecologia
https://www.readbyqxmd.com/read/29224115/ct-features-for-diagnosing-acute-torsion-of-uterine-subserosal-leiomyoma
#19
Yoshimitsu Ohgiya, Noritaka Seino, Shingo Miyamoto, Nobuyuki Takeyama, Kumi Hatano, Jiro Munechika, Kumiko Ishizuka, Masanori Hirose, Nobuyuki Ohike, Toshi Hashimoto, Akihiko Sekizawa, Takehiko Gokan
PURPOSE: To evaluate the usefulness of computed tomographic (CT) features for identifying acute torsion of uterine subserosal leiomyoma (USL). SUBJECTS AND METHODS: We analyzed contrast-enhanced CT examinations of 7 USLs with torsion and 44 USLs without torsion. Two radiologists evaluated the CT features, which consisted of poor contrast enhancement inside the USL, thin rim enhancement around the USL, calcification within the USL, a beak sign between the uterus and USL, fan-shaped poor contrast enhancement in the uterus area adjacent to the USL (dark fan sign), and ascites...
December 9, 2017: Japanese Journal of Radiology
https://www.readbyqxmd.com/read/29224114/coated-platelet-trends-predict-short-term-clinical-outcomeafter-subarachnoid-hemorrhage
#20
Bappaditya Ray, Vijay M Pandav, Eleanor A Mathews, David M Thompson, Lance Ford, Lori K Yearout, Bradley N Bohnstedt, Shuchi Chaudhary, George L Dale, Calin I Prodan
Aneurysmal subarachnoid hemorrhage (aSAH) is associated with high socio-economic burden. Prothrombotic states of early brain injury (EBI) and delayed cerebral ischemia (DCI) after aSAH determine morbidity and mortality. To understand how activated platelets might contribute to such prothrombotic states, we studied trends in coated-platelets during EBI and DCI periods. Serial blood samples from a prospective cohort of aSAH patients were collected and assayed for coated-platelet levels. Patient's coated-platelet level during post-hospital discharge follow-up served as an estimate of baseline...
December 9, 2017: Translational Stroke Research
keyword
keyword
5544
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"