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https://www.readbyqxmd.com/read/27933047/diazotroph-diversity-in-the-sea-ice-melt-ponds-and-surface-waters-of-the-eurasian-basin-of-the-central-arctic-ocean
#1
Mar Fernández-Méndez, Kendra A Turk-Kubo, Pier L Buttigieg, Josephine Z Rapp, Thomas Krumpen, Jonathan P Zehr, Antje Boetius
The Eurasian basin of the Central Arctic Ocean is nitrogen limited, but little is known about the presence and role of nitrogen-fixing bacteria. Recent studies have indicated the occurrence of diazotrophs in Arctic coastal waters potentially of riverine origin. Here, we investigated the presence of diazotrophs in ice and surface waters of the Central Arctic Ocean in the summer of 2012. We identified diverse communities of putative diazotrophs through targeted analysis of the nifH gene, which encodes the iron protein of the nitrogenase enzyme...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27933006/system-to-detect-racial-based-bullying-through-gamification
#2
José A Álvarez-Bermejo, Luis J Belmonte-Ureña, Africa Martos-Martínez, Ana B Barragán-Martín, María Del Mar Simón-Marquez
Prevention and detection of bullying due to racial stigma was studied in school contexts using a system designed following "gamification" principles and integrating less usual elements, such as social interaction, augmented reality and cell phones in educational scenarios. "Grounded Theory" and "User Centered Design" were employed to explore coexistence inside and outside the classroom in terms of preferences and distrust in several areas of action and social frameworks of activity, and to direct the development of a cell phone app for early detection of school bullying scenarios...
2016: Frontiers in Psychology
https://www.readbyqxmd.com/read/27932982/impact-of-cyp2c19-variants-on-clinical-efficacy-of-clopidogrel-and-1-year-clinical-outcomes-in-coronary-heart-patients-undergoing-percutaneous-coronary-intervention
#3
Hong Sun, Qiang Qu, Zhen-Fan Chen, Sheng-Lan Tan, Hai-Jun Zhou, Jian Qu, Hui Chen
The impact of pharmacogenetic variants of cytochrome P450 2C19 (CYP2C19) on clopidogrel-mediated effects on platelet inhibition, inflammatory response and endothelial function, as well as risk of major adverse cardiovascular events (MACE), in coronary heart patients undergoing percutaneous coronary intervention (PCI) was investigated. To this end, we assessed the residual platelet aggregation rate (RPA), maximal aggregation rate (MAR) and plasma levels of sCD40L, sP-selectin, MMP-9, sVCAM-1 and sE-selectin after 24 h of PCI in 559 patients treated with clopidogrel and followed up for 1 year for evidence of MACE...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27931207/a-comparative-study-classification-vs-user-based-collaborative-filtering-for-clinical-prediction
#4
Fang Hao, Rachael Hageman Blair
BACKGROUND: Recommender systems have shown tremendous value for the prediction of personalized item recommendations for individuals in a variety of settings (e.g., marketing, e-commerce, etc.). User-based collaborative filtering is a popular recommender system, which leverages an individuals' prior satisfaction with items, as well as the satisfaction of individuals that are "similar". Recently, there have been applications of collaborative filtering based recommender systems for clinical risk prediction...
December 8, 2016: BMC Medical Research Methodology
https://www.readbyqxmd.com/read/27931165/improvement-of-municipal-wastewater-pretreatment-by-direct-membrane-filtration
#5
Thiago A Nascimento, Fanny R Mejía, Fernando Fdz-Polanco, Mar Peña Miranda
The high content of particulate matter in municipal wastewater hinders the conventional anaerobic treatments at psychrophilic temperatures. The hydrolysis of the particulate chemical oxygen demand (pCOD) could be the limiting step under these conditions. Therefore, new pretreatments or improved conventional pretreatments are needed in order to separate pCOD. In this work, direct membrane filtration of municipal wastewater, using an ultrafiltration membrane, was investigated. This intensive pretreatment, which aims to separate soluble chemical oxygen demand (sCOD) and to concentrate pCOD, together with anaerobic treatments of both streams at psychrophilic and mesophilic conditions respectively, could be an alternative to the conventional activated sludge process...
December 8, 2016: Environmental Technology
https://www.readbyqxmd.com/read/27929520/the-minimum-area-requirements-mar-for-giant-panda-an-empirical-study
#6
Jing Qing, Zhisong Yang, Ke He, Zejun Zhang, Xiaodong Gu, Xuyu Yang, Wen Zhang, Biao Yang, Dunwu Qi, Qiang Dai
Habitat fragmentation can reduce population viability, especially for area-sensitive species. The Minimum Area Requirements (MAR) of a population is the area required for the population's long-term persistence. In this study, the response of occupancy probability of giant pandas against habitat patch size was studied in five of the six mountain ranges inhabited by giant panda, which cover over 78% of the global distribution of giant panda habitat. The probability of giant panda occurrence was positively associated with habitat patch area, and the observed increase in occupancy probability with patch size was higher than that due to passive sampling alone...
December 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27927590/emergency-radiology-practice-patterns-shifts-schedules-and-job-satisfaction
#7
Tarek N Hanna, Haris Shekhani, Christine Lamoureux, Hanna Mar, Nicola Refky, Clint Sliker, Jamlik-Omari Johnson
PURPOSE: To assess the practice environment of emergency radiologists with a focus on schedule, job satisfaction, and self-perception of health, wellness, and diagnostic accuracy. METHODS: A survey drawing from prior radiology and health care shift-work literature was distributed via e-mail to national societies, teleradiology groups, and private practices. The survey remained open for 4 weeks in 2016, with one reminder. Data were analyzed using hypothesis testing and logistic regression modeling...
December 4, 2016: Journal of the American College of Radiology: JACR
https://www.readbyqxmd.com/read/27927574/untreated-thoracic-curve-in-adult-idiopathic-scoliosis-what-are-patients-concerns
#8
Javier Pizones, Mar Pérez Martin-Buitrago, Francisco Javier Sánchez Perez-Grueso, Ferran Pellisé, Ahmet Alanay, Ibrahim Obeid, Frank Kleinstück, Emre R Acaroglu
STUDY DESIGN: Retrospective two-cohort comparative analysis of data collected prospectively. OBJECTIVES: To analyze a cohort of patients with untreated thoracic curves of an adult multicenter deformity database [European spine study group (ESSG)], describe patient characteristics and concerns, and establish the rate and motivations for surgical intervention. SUMMARY OF BACKGROUND DATA: Idiopathic thoracic curves have a significant clinical and socioeconomic impact during adolescence...
November 2016: Spine Deformity
https://www.readbyqxmd.com/read/27927036/integrative-group-based-cognitive-rehabilitation-efficacy-in-multiple-sclerosis-a-randomized-clinical-trial
#9
Oiane Rilo, Javier Peña, Natalia Ojeda, Alfredo Rodríguez-Antigüedad, Mar Mendibe-Bilbao, Ainara Gómez-Gastiasoro, John DeLuca, Nancy Chiaravalloti, Naroa Ibarretxe-Bilbao
PURPOSE: This study aimed to determine the efficacy of the integrative group-based cognitive rehabilitation programme, REHACOP, on improving cognitive functions in multiple sclerosis (MS). METHODS: Fourty-two MS patients were randomized to the treatment programme REHACOP (n = 21) or waiting list control condition (n = 21). The REHACOP group received cognitive rehabilitation in group format for three months focused on attention, processing speed, learning and memory, language, executive functioning, and social cognition...
December 7, 2016: Disability and Rehabilitation
https://www.readbyqxmd.com/read/27926769/myocardial-fibrosis-progression-in-duchenne-and-becker-muscular-dystrophy-a-randomized-clinical-trial
#10
Marly Conceição Silva, Tiago Augusto Magalhães, Zilda Maria Alves Meira, Carlos Henrique Reis Esselin Rassi, Amanda Cristina de Souza Andrade, Paulo Sampaio Gutierrez, Clerio Francisco Azevedo, Juliana Gurgel-Giannetti, Mariz Vainzof, Mayana Zatz, Roberto Kalil-Filho, Carlos Eduardo Rochitte
Importance: In Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), interventions reducing the progression of myocardial disease could affect survival. Objective: To assess the effect of early angiotensin-converting enzyme (ACE) inhibitor therapy in patients with normal left ventricular function on the progression of myocardial fibrosis (MF) identified on cardiovascular magnetic resonance (CMR). Design, Setting, and Participants: A randomized clinical trial conducted in 2 centers included 76 male patients with DMD or BMD undergoing 2 CMR studies with a 2-year interval for ventricular function and MF assessment...
December 7, 2016: JAMA Cardiology
https://www.readbyqxmd.com/read/27925795/oxidants-at-the-surface-of-mars-a-review-in-light-of-recent-exploration-results
#11
J Lasne, A Noblet, C Szopa, R Navarro-González, M Cabane, O Poch, F Stalport, P François, S K Atreya, P Coll
In 1976, the Viking landers carried out the most comprehensive search for organics and microbial life in the martian regolith. Their results indicate that Mars' surface is lifeless and, surprisingly, depleted in organics at part-per-billion levels. Several biology experiments on the Viking landers gave controversial results that have since been explained by the presence of oxidizing agents on the surface of Mars. These oxidants may degrade abiotic or biological organics, resulting in their nondetection in the regolith...
December 7, 2016: Astrobiology
https://www.readbyqxmd.com/read/27925426/the-impact-of-home-fortification-with-multiple-micronutrient-powder-on-vitamin-a-status-in-young-children-a-multicenter-pragmatic-controlled-trial-in-brazil
#12
Lara Livia Santos Silva, Rosângela Aparecida Augusto, Daniela Cardoso Tietzmann, Leopoldina Augusta Souza Sequeira, Maria Claret Costa Monteiro Hadler, Pascoal Torres Muniz, Pedro Israel Cabral de Lira, Marly Augusto Cardoso
Home fortification with multiple micronutrient powder (MNP) is effective in the prevention of anemia in young children. However, the impact on their vitamin A status remains controversial. This study aimed to evaluate the effect of MNP on vitamin A status in young Brazilian children. A multicenter pragmatic, controlled trial was carried out in primary health centers in four Brazilian cities. In the beginning of the study, the control group (CG) consisted of children 11-14 months old (n = 395) attending in routine pediatric health care...
December 7, 2016: Maternal & Child Nutrition
https://www.readbyqxmd.com/read/27925386/interferon-free-therapies-for-patients-with-chronic%C3%A2-hepatitis-c-genotype-3-infection-a-systematic-review
#13
Vicente Gimeno-Ballester, María Buti, Ramón San Miguel, Mar Riveiro, Rafael Esteban
INTRODUCTION: Treatment of hepatitis C virus (HCV) infection with genotype 3 remains a challenge. The HCV elimination rate with direct acting antivirals (DAAs) is lower than the values reported for other HCV genotypes. In addition, genotype 3 infected patients have a higher risk of disease progression and hepatocellular carcinoma. The aim of this study was to review the relevant literature concerning treatment of HCV genotype 3 patients with interferon-free regimens. MATERIAL AND METHODS: A literature search was conducted in the PubMed/Medline, Embase, and Web of Science electronic databases...
December 7, 2016: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/27925107/characteristics-of-motorcyclists-involved-in-road-traffic-accidents-attended-at-public-urgent-and-emergency-services
#14
Márcio Dênis Medeiros Mascarenhas, Rayone Moreira Costa Veloso Souto, Deborah Carvalho Malta, Marta Maria Alves da Silva, Cheila Marina de Lima, Marli de Mesquita Silva Montenegro
Injuries resulting from motorcycle road traffic accidents are an important public health issue in Brazil. This study aimed to describe the characteristics of motorcyclists involved in traffic accidents attended in public urgent and emergency services in the state capitals and the Federal District. This is a cross-sectional study based on data from the Violence and Accident Surveillance System (VIVA Survey) in 2014. Data were analyzed according to sociodemographic, event and attendance characteristics. Proportional differences between genders were analyzed by chi-square test (Rao-Scott) with 5% significance level...
December 2016: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/27924546/multiplex-detection-of-toxigenic-penicillium-species
#15
Alicia Rodríguez, Juan J Córdoba, Mar Rodríguez, María J Andrade
Multiplex PCR-based methods for simultaneous detection and quantification of different mycotoxin-producing Penicillia are useful tools to be used in food safety programs. These rapid and sensitive techniques allow taking corrective actions during food processing or storage for avoiding accumulation of mycotoxins in them. In this chapter, three multiplex PCR-based methods to detect at least patulin- and ochratoxin A-producing Penicillia are detailed. Two of them are different multiplex real-time PCR suitable for monitoring and quantifying toxigenic Penicillium using the nonspecific dye SYBR Green and specific hydrolysis probes (TaqMan)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924541/targeting-other-mycotoxin-biosynthetic-genes
#16
María J Andrade, Mar Rodríguez, Juan J Córdoba, Alicia Rodríguez
Real-time PCR (qPCR) methods are adequate tools for sensitive and rapid detection and quantification of toxigenic molds contaminating food commodities. Methods of qPCR for quantifying zearalenone (ZEA)-, sterigmatocystin (ST)-, cyclopiazonic acid (CPA)-, and patulin (PAT)-producing molds have been designed on the basis of specific target genes involved in the biosynthesis of these mycotoxins. In this chapter reliable qPCR protocols to detect and quantify such toxigenic molds are described. All of these methods are suitable when working with mold pure cultures and mold contaminated foods...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924426/preditor-a-synthetic-biology-approach-to-removing-heterochromatin-from-cells
#17
Oscar Molina, Mar Carmena, Isabella E Maudlin, William C Earnshaw
It is widely accepted that heterochromatin is necessary to maintain genomic stability. However, direct experimental evidence supporting this is slim. Previous studies using either enzyme inhibitors, gene knockout or knockdown studies all are subject to the caveat that drugs may have off-target effects and enzymes that modify chromatin proteins to support heterochromatin formation may also have numerous other cellular targets as well. Here, we describe PREditOR (protein reading and editing of residues), a synthetic biology approach that allows us to directly remove heterochromatin from cells without either drugs or global interference with gene function...
December 6, 2016: Chromosome Research
https://www.readbyqxmd.com/read/27924316/discovery-of-febi2
#18
James P S Walsh, Samantha M Clarke, Yue Meng, Steven D Jacobsen, Danna E Freedman
Recent advances in high-pressure techniques offer chemists access to vast regions of uncharted synthetic phase space, expanding our experimental reach to pressures comparable to the core of the Earth. These newfound capabilities enable us to revisit simple binary systems in search of compounds that for decades have remained elusive. The most tantalizing of these targets are systems in which the two elements in question do not interact even as molten liquids-so-called immiscible systems. As a prominent example, immiscibility between iron and bismuth is so severe that no material containing Fe-Bi bonds is known to exist...
November 23, 2016: ACS Central Science
https://www.readbyqxmd.com/read/27924313/a-compartmentalized-out-of-equilibrium-enzymatic-reaction-network-for-sustained-autonomous-movement
#19
Marlies Nijemeisland, Loai K E A Abdelmohsen, Wilhelm T S Huck, Daniela A Wilson, Jan C M van Hest
Every living cell is a compartmentalized out-of-equilibrium system exquisitely able to convert chemical energy into function. In order to maintain homeostasis, the flux of metabolites is tightly controlled by regulatory enzymatic networks. A crucial prerequisite for the development of lifelike materials is the construction of synthetic systems with compartmentalized reaction networks that maintain out-of-equilibrium function. Here, we aim for autonomous movement as an example of the conversion of feedstock molecules into function...
November 23, 2016: ACS Central Science
https://www.readbyqxmd.com/read/27923950/results-of-a-zika-virus-zikv-immunoglobulin-m-specific-diagnostic-assay-are-highly-correlated-with-detection-of-neutralizing-anti-zikv-antibodies-in-neonates-with-congenital-disease
#20
Marli T Cordeiro, Carlos A A Brito, Lindomar J Pena, Priscila M S Castanha, Laura H V G Gil, Kennya G S Lopes, Rafael Dhalia, Jucille A Meneses, Ana C Ishigami, Luisa M Mello, Liciana X E Alencar, Klarissa M Guarines, Laura C Rodrigues, Ernesto T A Marques
BACKGROUND:  Usually, immunoglobulin M (IgM) serologic analysis is not sufficiently specific to confirm Zika virus (ZIKV) infection. However, since IgM does not cross the placenta, it may be a good marker of infection in neonates. METHODS:  We tested blood from 42 mothers and neonates with microcephaly and collected cerebrospinal fluid (CSF) specimens from 30 neonates. Molecular assays were performed for detection of ZIKV, dengue virus, and chikungunya virus; IgM enzyme-linked immunosorbent assays and plaque-reduction neutralization tests (PRNTs) were performed to detect ZIKV and dengue virus...
December 15, 2016: Journal of Infectious Diseases
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