keyword
MENU ▼
Read by QxMD icon Read
search

Double robustness

keyword
https://www.readbyqxmd.com/read/28805376/discovery-and-biosensing-applications-of-diverse-rna-cleaving-dnazymes
#1
Meng Liu, Dingran Chang, Yingfu Li
DNA-based enzymes, or DNAzymes, are not known to exist in Nature but can be isolated from random-sequence DNA pools using test tube selection techniques. Since the report of the first DNAzyme in 1994, many catalytic DNA molecules for catalyzing wide-ranging chemical transformations have been isolated and studied. Our laboratory has a keen interest in searching for diverse DNAzymes capable of cleaving RNA-containing substrates, determining their sequence requirements and structural properties, and examining their potential as biosensors...
August 14, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28802674/the-yusuf-peto-method-was-not-a-robust-method-for-meta-analyses-of-rare-events-data-from-antidepressant-trials
#2
Tarang Sharma, Peter C Gøtzsche, Oliver Kuss
OBJECTIVE: To identify the validity of effect estimates for serious rare adverse events in clinical study reports of antidepressants trials, across different meta-analysis methods. STUDY DESIGN AND SETTING: Four serious rare adverse events (all-cause mortality, suicidality, aggressive behaviour and akathisia) were meta-analysed using different methods. The Yusuf-Peto odds ignores studies with no events, was compared with the alternative approaches of generalised linear mixed models (GLMM), conditional logistic regression, a Bayesian approach using Markov Chain Monte Carlo (MCMC) and a beta-binomial regression model...
August 9, 2017: Journal of Clinical Epidemiology
https://www.readbyqxmd.com/read/28793308/genetic-analysis-of-the-candida-albicans-biofilm-transcription-factor-network-using-simple-and-complex-haploinsufficiency
#3
Virginia E Glazier, Thomas Murante, Daniel Murante, Kristy Koselny, Yuan Liu, Dongyeop Kim, Hyun Koo, Damian J Krysan
Biofilm formation by Candida albicans is a key aspect of its pathobiology and is regulated by an integrated network of transcription factors (Bcr1, Brg1, Efg1, Ndt80, Rob1, and Tec1). To understand the details of how the transcription factors function together to regulate biofilm formation, we used a systematic genetic interaction approach based on generating all possible double heterozygous mutants of the network genes and quantitatively analyzing the genetic interactions between them. Overall, the network is highly susceptible to genetic perturbation with the six network heterozygous mutants all showing alterations in biofilm formation (haploinsufficiency)...
August 9, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28793147/comparing-public-safety-outcomes-for-traditional-probation-vs-specialty-mental-health-probation
#4
Jennifer L Skeem, Sarah Manchak, Lina Montoya
Importance: Probation is a cornerstone of efforts to reduce mass incarceration. Although it is understudied, specialty probation could improve outcomes for the overrepresented group of people with mental illness. Objective: To test whether specialty probation yields better public safety outcomes than traditional probation. Design, Setting, and Participants: A longitudinal observational study with group matching on age, sex, race/ethnicity, probation time, and offense at 2 urban agencies that exemplify specialty and traditional probation...
August 9, 2017: JAMA Psychiatry
https://www.readbyqxmd.com/read/28791791/functional-molecular-junctions-derived-from-double-self-assembled-monolayers
#5
Sohyeon Seo, Eunhee Hwang, Yunhee Cho, Junghyun Lee, Hyoyoung Lee
Information processing through molecular junctions is becoming more important as devices are miniaturized to nanoscale. Herein, we report functional molecular junctions derived from double self-assembled monolayers (SAMs) intercalated between soft graphene electrodes. Newly assembled molecular junctions are fabricated by placing one molecular SAM/(top) electrode on another molecular SAM/(bottom) electrode via a contact-assembly technique. Double SAMs can provide tunnelling conjugation across the van der Waals gap between the terminals of each monolayer and exhibit new electrical functions...
August 9, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28780332/controlled-fabrication-of-multi-core-alginate-microcapsules
#6
Md Danish Eqbal, Venkat Gundabala
In this work, we present a robust microfluidic platform for controlled and complete on-chip generation of alginate microcapsules with single and double liquid cores. A combined Coflow and T-junction configuration implemented in a hybrid glass-PDMS (Polydimethylsiloxane) device is used for the generation of microcapsules with oil as liquid core. Frequency matching of oil-alginate double emulsion generation with that of aqueous Calcium chloride droplet generation allows for controlled merging of the two, resulting in reliable production of microcapsules...
July 28, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28777557/double-solvent-sensing-method-for-improving-sensitivity-and-accuracy-of-hg-ii-detection-based-on-different-signal-transduction-of-a-tetrazine-functionalized-pillared-metal-organic-framework
#7
Sayed Ali Akbar Razavi, Mohammad Yaser Masoomi, Ali Morsali
To design a robust, π-conjugated, low-cost, and easy to synthesize metal-organic framework (MOF) for cation sensing by the photoluminescence (PL) method, 4,4'-oxybis(benzoic acid) (H2OBA) has been used in combination with 3,6-di(pyridin-4-yl)-1,2,4,5-tetrazine (DPT) as a tetrazine-functionalized spacer to construct [Zn(OBA)(DPT)0.5]·DMF (TMU-34(-2H)). The tetrazine motif is a π-conjugated, water-soluble/stable fluorophore with relatively weak σ-donating Lewis basic sites. These characteristics of tetrazine make TMU-34(-2H) a good candidate for cation sensing...
August 4, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28772041/budget-impact-of-intravenous-iron-therapy-with-ferric-carboxymaltose-in-patients-with-chronic-heart-failure-and-iron-deficiency-in-germany
#8
Ulrike Theidel, Saku Väätäinen, Janne Martikainen, Erkki Soini, Thomas Hardt, Wolfram Doehner
AIMS: Treatment of iron deficiency (ID) in patients with heart failure (HF) with intravenous iron substitution [ferric carboxymaltose (FCM)] has previously shown significant improvements in exercise capacity, New York Heart Association (NYHA) functional class, quality of life, and reduction of hospitalization. The aim of this study was to estimate the budget impact of FCM treatment for patients with HF and ID. METHODS AND RESULTS: Individual patient data from four double-blind randomized controlled trials were pooled for this analysis...
August 2017: ESC Heart Failure
https://www.readbyqxmd.com/read/28768872/high-risk-alpha-papillomavirus-oncogenes-impair-the-homologous-recombination-pathway
#9
Nicholas A Wallace, Sujita Khanal, Kristin L Robinson, Sebastian O Wendel, Joshua J Messer, Denise A Galloway
Persistent high risk genus α human papillomavirus (HPV) infections cause nearly every cervical carcinoma and a subset of tumors in the oropharyngeal tract. During the decades required for HPV-associated tumorigenesis, the cellular genome becomes significantly destabilized. Our analysis of cervical tumors from 4 separate data sets found a significant upregulation of the homologous recombination (HR) pathway genes. The increased abundance of HR proteins can be replicated in primary cells by expression of the two HPV oncogenes (E6 and E7) required for HPV-associated transformation...
August 2, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28765523/robust-spin-correlations-at-high-magnetic-fields-in-the-harmonic-honeycomb-iridates
#10
K A Modic, B J Ramshaw, J B Betts, Nicholas P Breznay, James G Analytis, Ross D McDonald, Arkady Shekhter
The complex antiferromagnetic orders observed in the honeycomb iridates are a double-edged sword in the search for a quantum spin-liquid: both attesting that the magnetic interactions provide many of the necessary ingredients, while simultaneously impeding access. Focus has naturally been drawn to the unusual magnetic orders that hint at the underlying spin correlations. However, the study of any particular broken symmetry state generally provides little clue about the possibility of other nearby ground states...
August 1, 2017: Nature Communications
https://www.readbyqxmd.com/read/28765214/epigenetic-resetting-of-human-pluripotency
#11
Ge Guo, Ferdinand von Meyenn, Maria Rostovskaya, James Clarke, Sabine Dietmann, Duncan Baker, Anna Sahakyan, Samuel Myers, Paul Bertone, Wolf Reik, Kathrin Plath, Austin Smith
Much attention has focussed on the conversion of human pluripotent stem cells (PSCs) to a more naïve developmental status. Here we provide a method for resetting via transient histone deacetylase inhibition. The protocol is effective across multiple PSC lines and can proceed without karyotype change. Reset cells can be expanded without feeders with a doubling time of around 24 h. WNT inhibition stabilises the resetting process. The transcriptome of reset cells diverges markedly from that of primed PSCs and shares features with human inner cell mass (ICM)...
August 1, 2017: Development
https://www.readbyqxmd.com/read/28764716/generation-of-transgenic-rodent-malaria-parasites-by-transfection-of-cell-culture-derived-merozoites
#12
Gesine Kaiser, Mariana De Niz, Paul-Christian Burda, Livia Niklaus, Rebecca Limenitakis Stanway, Volker Heussler
BACKGROUND: Malaria research is greatly dependent on and has drastically advanced with the possibility of genetically modifying Plasmodium parasites. The commonly used transfection protocol by Janse and colleagues utilizes blood stage-derived Plasmodium berghei schizonts that have been purified from a blood culture by density gradient centrifugation. Naturally, this transfection protocol depends on the availability of suitably infected mice, constituting a time-based variable. In this study, the potential of transfecting liver stage-derived merozoites was explored...
August 1, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28764137/modeling-the-aqueous-phase-reactivity-of-hydroxyl-radical-towards-diverse-organic-micropollutants-an-aid-to-water-decontamination-processes
#13
Shikha Gupta, Nikita Basant
The rate constants of the hydroxyl radical reactions (kOH) with organic micropollutants (OMPs) in aqueous medium are important in designing the advanced oxidation processes (AOPs) for their removal. In this study, a quantitative structure-property relationship (QSPR) model for the prediction of kOH of diverse and emerging OMPs was developed in accordance with the OECD guidelines. A large experimental data set (n = 995) comprised of compounds with kOH values ranging from 7.9 × 10(5) to 6.8 × 10(10) M(-1) s(-1) was considered and several molecular descriptors were calculated...
July 14, 2017: Chemosphere
https://www.readbyqxmd.com/read/28761145/large-scale-differences-in-microbial-biodiversity-discovery-between-16s-amplicon-and-shotgun-sequencing
#14
Michael Tessler, Johannes S Neumann, Ebrahim Afshinnekoo, Michael Pineda, Rebecca Hersch, Luiz Felipe M Velho, Bianca T Segovia, Fabio A Lansac-Toha, Michael Lemke, Rob DeSalle, Christopher E Mason, Mercer R Brugler
Modern metagenomic environmental DNA studies are almost completely reliant on next-generation sequencing, making evaluations of these methods critical. We compare two next-generation sequencing techniques - amplicon and shotgun - on water samples across four of Brazil's major river floodplain systems (Amazon, Araguaia, Paraná, and Pantanal). Less than 50% of phyla identified via amplicon sequencing were recovered from shotgun sequencing, clearly challenging the dogma that mid-depth shotgun recovers more diversity than amplicon-based approaches...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28760205/probing-oral-anticoagulation-in-patients-with-atrial-high-rate-episodes-rationale-and-design-of-the-non-vitamin-k-antagonist-oral-anticoagulants-in-patients-with-atrial-high-rate-episodes-noah-afnet-6-trial
#15
Paulus Kirchhof, Benjamin F Blank, Melanie Calvert, A John Camm, Gregory Chlouverakis, Hans-Christoph Diener, Andreas Goette, Andrea Huening, Gregory Y H Lip, Emmanuel Simantirakis, Panos Vardas
Oral anticoagulation prevents ischemic strokes in patients with atrial fibrillation (AF). Early detection of AF and subsequent initiation of oral anticoagulation help to prevent strokes in AF patients. Implanted cardiac pacemakers and defibrillators allow seamless detection of atrial high rate episodes (AHRE), but the best antithrombotic therapy in patients with AHRE is not known. RATIONALE: Stroke risk is higher in pacemaker patients with AHRE than in those without, but the available data also show that stroke risk in patients with AHRE is lower than in patients with AF...
August 2017: American Heart Journal
https://www.readbyqxmd.com/read/28755550/impact-of-early-life-adversity-and-tryptophan-depletion-on-functional-connectivity-in-menopausal-women-a-double-blind-placebo-controlled-crossover-study
#16
Sheila Shanmugan, Theodore D Satterthwaite, Mary D Sammel, Wen Cao, Kosha Ruparel, Ruben C Gur, C Neill Epperson, James Loughead
During the menopause transition, women are at increased risk of subjective symptoms of executive dysfunction. Evidence from animal and human participant studies suggests adverse childhood experiences (ACE) may be a risk factor for executive complaints during this hormonal transition. Preclinical literature indicates early life adversity effects on serotonin function may play a role in this increased susceptibility. However, the mechanisms underlying this increase in vulnerability in human participants remain relatively unknown...
July 14, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28754551/the-fragility-of-significant-results-underscores-the-need-of-larger-randomized-controlled-trials-in-nephrology
#17
Lani R Shochet, Peter G Kerr, Kevan R Polkinghorne
The Fragility Index is a tool for testing robustness of randomized controlled trial results for dichotomous outcomes. It describes the minimum number of individuals in whom changing an event status would render a statistically significant result nonsignificant. Here we identified all randomized controlled trials in five nephrology and five general journals from 2005-2014. A total of 127 randomized controlled trials reporting at least one dichotomous statistically significant outcome (p less than 0.05) were included and the Fragility Index was calculated...
July 26, 2017: Kidney International
https://www.readbyqxmd.com/read/28754262/a-comparison-of-three-peyer-s-patch-m-like-cell-culture-models-particle-uptake-bacterial-interaction-and-epithelial-histology
#18
Tauseef Ahmad, Martina Gogarty, Edwin Walsh, David J Brayden
Intestinal Peyer's patch (PP) microfold (M) cells transport microbes and particulates across the follicle-associated epithelium (FAE) as part of the mucosal immune surveillance system. In vitro human M-like cell co-culture models are used as screens to investigate uptake of antigens-in-nanoparticles, but the models are labour-intensive and there is inter-laboratory variability. We compared the three most established filter-grown Caco-2/Raji B cell co-culture systems. These were Model A (Kerneis et al, 1997), Model B (Gullberg et al...
July 25, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28752624/paired-motor-cortex-and-cervical-epidural-electrical-stimulation-timed-to-converge-in-the-spinal-cord-promotes-lasting-increases-in-motor-responses
#19
Asht M Mishra, Ajay Pal, Disha Gupta, Jason B Carmel
Convergent activity in neural circuits can generate changes at their intersection. The rules of paired electrical stimulation are best understood for paradigms that stimulate input circuits and their targets. We took a different approach by targeting the interaction of descending motor pathways and large-diameter afferents in the spinal cord. We hypothesized that pairing stimulation of motor cortex and cervical spinal cord would strengthen motor responses through their convergence. We placed epidural electrodes over motor cortex and the dorsal cervical spinal cord in rats; motor evoked potentials (MEPs) were measured from biceps...
July 28, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28750535/evaluation-of-dna-methyltransferase-activity-and-inhibition-via-isothermal-enzyme-free-concatenated-hybridization-chain-reaction
#20
Qing Wang, Min Pan, Jie Wei, Xiaoqing Liu, Fuan Wang
Methyltransferase (MTase)-catalyzed DNA methylation plays a vital role in the biological epigenetic processes of key diseases and has attracted increasing attention, making the amplified detection of MTase activity of great significance in clinical disease diagnosis and treatment. Herein, we developed an isothermal, enzyme-free, and autonomous strategy for analyzing MTase activity based on concatenated hybridization chain reaction (C-HCR)-mediated Förster resonance energy transfer (FRET). In a typical C-HCR procedure without MTase (Dam), Y-shaped initiator DNA activates upstream HCR-1 to assemble a double-stranded DNA (dsDNA) copolymeric nanowire consisting of multiple tandem DNA trigger units that motivate downstream HCR-2 to successively bring a fluorophore donor/acceptor (FAM/TAMRA) pair into close proximity, leading to the generation of an amplified FRET readout signal...
July 28, 2017: ACS Sensors
keyword
keyword
55429
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"