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https://www.readbyqxmd.com/read/29331340/hsp72-protects-from-liver-injury-via-attenuation-of-hepatocellular-death-oxidative-stress-and-jnk-signaling
#1
Kateryna Levada, Nurdan Guldiken, Xiaoji Zhang, Giovanna Vella, Fa-Rong Mo, Laura P James, Johannes Haybaeck, Sonja M Kessler, Alexandra K Kiemer, Thomas Ott, Daniel Hartmann, Norbert Hüser, Marianne Ziol, Christian Trautwein, Pavel Strnad
BACKGROUND AND AIMS: Heat shock protein (Hsp) 72 is a molecular chaperone that is upregulated in response to stress and possesses broad cytoprotective functions. To determine its hepatic function, we studied its expression in human liver disorders and its biological significance in newly generated transgenic animals. AIM AND METHODS: Double transgenic mice overexpressing Hsp72 (gene HSPA1A) under the control of a tissue-specific tetracycline-inducible system (Hsp72-LAP mice) were produced...
January 10, 2018: Journal of Hepatology
https://www.readbyqxmd.com/read/29326271/ordered-macro-microporous-metal-organic-framework-single-crystals
#2
Kui Shen, Lei Zhang, Xiaodong Chen, Lingmei Liu, Daliang Zhang, Yu Han, Junying Chen, Jilan Long, Rafael Luque, Yingwei Li, Banglin Chen
We constructed highly oriented and ordered macropores within metal-organic framework (MOF) single crystals, opening up the area of three-dimensional-ordered macro-microporous materials (that is, materials containing both macro- and micropores) in single-crystalline form. Our methodology relies on the strong shaping effects of a polystyrene nanosphere monolith template and a double-solvent-induced heterogeneous nucleation approach. This process synergistically enabled the in situ growth of MOFs within ordered voids, rendering a single crystal with oriented and ordered macro-microporous structure...
January 12, 2018: Science
https://www.readbyqxmd.com/read/29326077/mechanism-of-nanoparticle-induced-hypersensitivity-in-pigs-complement-or-not-complement
#3
János Szebeni
A recent study on nanoparticle-induced hypersensitivity reactions in pigs showed robust pulmonary intravascular macrophage clearance of Polybead® carboxylate microspheres in mediating the adverse cardiopulmonary distress, irrespective of the ability of these particles to activate the Complement (C) system in vitro. Focusing on this observation, this article highlights the controversies in projecting in vitro C assay data to in vivo conditions and applying data on polystyrene particles to therapeutic nanopharmaceuticals...
January 8, 2018: Drug Discovery Today
https://www.readbyqxmd.com/read/29324605/estimating-the-hospital-delivery-costs-associated-with-severe-maternal-morbidity-in-new-york-city-2008-2012
#4
Renata E Howland, Meghan Angley, Sang Hee Won, Wendy Wilcox, Hannah Searing, Tsu-Yu Tsao
OBJECTIVE: To quantify the average and total hospital delivery costs associated with severe maternal morbidity in excess of nonsevere maternal morbidity deliveries over a 5-year period in New York City adjusting for other sociodemographic and clinical factors. METHODS: We conducted a population-based cross-sectional study using linked birth certificates and hospital discharge data for New York City deliveries from 2008 to 2012. Severe maternal morbidity was defined using a published algorithm of International Classification of Diseases, 9 Revision, Clinical Modification disease and procedure codes...
January 9, 2018: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29319907/aberrant-accumulation-of-erbb4-in-progressive-supranuclear-palsy
#5
Aya Murakami, Masataka Nakamura, Satoshi Kaneko, Wen-Lang Lin, Dennis W Dickson, Hirofumi Kusaka
The human epidermal growth factor receptor family consists of 4 members that belong to the ErbB lineage of proteins (ErbB1-4). Neuregulin-1 (NRG1)/ErbB signalling regulates brain development and function. Abnormalities in this signalling have been implicated in the aetiology or development of neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis. So, we aimed at investigating whether the expression of NRG1 or ErbB proteins are altered in progressive supranuclear palsy (PSP) METHODS: The brains of 10 PSP and 6 control patients were investigated by immunohistochemical analysis RESULTS: Whereas C-terminal ErbB4 immunoreacitivity was partially but distinctly present in the cytoplasm and/or in the nucleus of neurons in control patients, it was rarely observed in the neuronal nuclei in PSP patients...
January 10, 2018: Neuropathology and Applied Neurobiology
https://www.readbyqxmd.com/read/29317495/structural-and-mechanistic-analysis-of-a-%C3%AE-glycoside-phosphorylase-identified-by-screening-a-metagenomic-library
#6
Spencer S Macdonald, Ankoor Patel, Veronica L C Larmour, Connor Morgan-Lang, Steven J Hallam, Brian L Mark, Stephen G Withers
Glycoside phosphorylases have considerable potential as catalysts for the assembly of useful glycans for products ranging from functional foods and prebiotics to novel materials. However, the substrate diversity of currently identified phosphorylases is relatively small, limiting their practical applications. To address this limitation, we developed a high-throughput screening approach using the activated substrate 2,4-dinitrophenyl β-D-glucoside (DNPGlc) and inorganic phosphate for identifying glycoside phosphorylase activity and used it to screen a large insert metagenomic library...
January 9, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29317186/perturbation-of-theta-gamma-coupling-at-the-temporal-lobe-hinders-verbal-declarative-memory
#7
Gabriel Amador de Lara, Ivan Alekseichuk, Zsolt Turi, Albert Lehr, Andrea Antal, Walter Paulus
BACKGROUND: Phase-amplitude cross-frequency coupling (PAC) is characterized by the modulation of the power of a fast brain oscillation (e.g., gamma) by the phase of a slow rhythm (e.g., theta). PAC in different sub- and neocortical regions is known to underlie effective neural communication and correlates with successful long-term memory formation. OBJECTIVE/HYPOTHESIS: The present work aims to extend earlier observational data, by probing the functional role of theta-gamma PAC in the left temporal cortex in humans during verbal long-term memory encoding...
December 25, 2017: Brain Stimulation
https://www.readbyqxmd.com/read/29315959/morphological-and-molecular-redefinition-of-euplotes-platystoma-dragesco-dragesco-kern%C3%A3-is-1986-and-aspidisca-lynceus-m%C3%A3-ller-1773-ehrenberg-1830-with-reconsideration-of-a-well-known-euplotes-ciliate-euplotes-harpa-stein-1859-ciliophora-euplotida
#8
Chunyu Lian, Xiaotian Luo, Xinpeng Fan, Jie Huang, Yuhe Yu, William Bourland, Weibo Song
We documented the morphology, infraciliature, silverline system and molecular data of two euplotid species isolated from China, including two populations of the poorly known Euplotes platystoma Dragesco & Dragesco-Kernéis, 1986 and the previously well described Aspidisca lynceus (Müller, 1773) Ehrenberg, 1830. Based on the information available, an improved diagnosis of Euplotes platystoma is given, including: a narrow adoral zone with 44-68 membranelles, ten frontoventral, five transverse, two left marginal and two caudal cirri, 11-13 dorsal kineties with 17-25 dikinetids in the mid-dorsal row, and dorsal silverline system of the double-eurystomus type...
January 9, 2018: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/29311308/dna-double-strand-break-response-factors-influence-end-joining-features-of-igh-class-switch-and-general-translocation-junctions
#9
Rohit A Panchakshari, Xuefei Zhang, Vipul Kumar, Zhou Du, Pei-Chi Wei, Jennifer Kao, Junchao Dong, Frederick W Alt
Ig heavy chain (IgH) class switch recombination (CSR) in B lymphocytes switches IgH constant regions to change antibody functions. CSR is initiated by DNA double-strand breaks (DSBs) within a donor IgH switch (S) region and a downstream acceptor S region. CSR is completed by fusing donor and acceptor S region DSB ends by classical nonhomologous end-joining (C-NHEJ) and, in its absence, by alternative end-joining that is more biased to use longer junctional microhomologies (MHs). Deficiency for DSB response (DSBR) factors, including ataxia telangiectasia-mutated (ATM) and 53BP1, variably impair CSR end-joining, with 53BP1 deficiency having the greatest impact...
January 8, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29307067/benchmarking-the-efficiency-of-the-chilean-water-and-sewerage-companies-a-double-bootstrap-approach
#10
María Molinos-Senante, Guillermo Donoso, Ramon Sala-Garrido, Andrés Villegas
Benchmarking the efficiency of water companies is essential to set water tariffs and to promote their sustainability. In doing so, most of the previous studies have applied conventional data envelopment analysis (DEA) models. However, it is a deterministic method that does not allow to identify environmental factors influencing efficiency scores. To overcome this limitation, this paper evaluates the efficiency of a sample of Chilean water and sewerage companies applying a double-bootstrap DEA model. Results evidenced that the ranking of water and sewerage companies changes notably whether efficiency scores are computed applying conventional or double-bootstrap DEA models...
January 6, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29302713/psilocybin-disrupts-sensory-and-higher-order-cognitive-processing-but-not-pre-attentive-cognitive-processing-study-on-p300-and-mismatch-negativity-in-healthy-volunteers
#11
Anna Bravermanová, Michaela Viktorinová, Filip Tylš, Tomáš Novák, Renáta Androvičová, Jakub Korčák, Jiří Horáček, Marie Balíková, Inga Griškova-Bulanova, Dominika Danielová, Přemysl Vlček, Pavel Mohr, Martin Brunovský, Vlastimil Koudelka, Tomáš Páleníček
RATIONALE: Disruption of auditory event-related evoked potentials (ERPs) P300 and mismatch negativity (MMN), electrophysiological markers of attentive and pre-attentive cognitive processing, is repeatedly described in psychosis and schizophrenia. Similar findings were observed in a glutamatergic model of psychosis, but the role of serotonergic 5-HT2A receptors in information processing is less clear. OBJECTIVES: We studied ERPs in a serotonergic model of psychosis, induced by psilocybin, a psychedelic with 5-HT2A/C agonistic properties, in healthy volunteers...
January 5, 2018: Psychopharmacology
https://www.readbyqxmd.com/read/29301234/the-effect-of-coatings-and-nerve-growth-factor-on-attachment-and-differentiation-of-pheochromocytoma-cells
#12
Anna Orlowska, Pallale Tharushi Perera, Mohammad Al Kobaisi, Andre Dias, Huu Khuong Duy Nguyen, Shahram Ghanaati, Vladimir Baulin, Russell J Crawford, Elena P Ivanova
Cellular attachment plays a vital role in the differentiation of pheochromocytoma (PC12) cells. PC12 cells are noradrenergic clonal cells isolated from the adrenal medulla of Rattus norvegicus and studied extensively as they have the ability to differentiate into sympathetic neuron-like cells. The effect of several experimental parameters including (i) the concentration of nerve growth factor (NGF); (ii) substratum coatings, such as poly-L-lysine (PLL), fibronectin (Fn), and laminin (Lam); and (iii) double coatings composed of PLL/Lam and PLL/Fn on the differentiation process of PC12 cells were studied...
December 31, 2017: Materials
https://www.readbyqxmd.com/read/29288453/serial-dilution-based-growth-curves-and-growth-curve-synchronization-for-high-resolution-time-series-of-bacterial-biofilm-growth
#13
Fernando Govantes
The ability to form stable surface-attached communities called biofilms is of paramount importance to both beneficial and harmful interactions between microbes and microbial, plant or animal partners. Assessment of biofilm formation ability is often performed by growing the organisms in microtiter plate wells and staining the well-attached material, a method whose use for time-course analysis is limited by its destructive nature. Here we combine a serial dilution-based biofilm growth curve method with online monitoring of planktonic growth and a serially diluted growth curve synchronization algorithm to reconstruct the time-course of planktonic and biofilm growth...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29284036/effect-of-innate-antiviral-glycoproteins-in-breast-milk-on-seroconversion-to-rotavirus-vaccine-rotarix-in-children-in-lusaka-zambia
#14
Katayi Mwila-Kazimbaya, Miguel Pugliese Garcia, Samuel Bosomprah, Natasha Makabilo Laban, Caroline Cleopatra Chisenga, Sallie Robey Permar, Michelo Simuyandi, Sody Munsaka, Roma Chilengi
INTRODUCTION: Rotavirus vaccines have been introduced into national immunization programmes to mitigate morbidity and mortality associated rotavirus diarrhoea. Lower vaccine effectiveness has however been noted in low-middle income countries, but little is known about the role of maternal components found in breast milk. This study assessed the effect of lactoferrin, lactadherin, and tenascin-c on rotavirus vaccine seroconversion. METHODS: This was a retrospective cohort study of 128 infants who had been fully immunized with Rotarix™...
2017: PloS One
https://www.readbyqxmd.com/read/29281659/immunogenicity-of-rotavirus-vaccine-rotarixtm-in-infants-with-environmental-enteric-dysfunction
#15
Innocent Mwape, Samuel Bosomprah, John Mwaba, Katayi Mwila-Kazimbaya, Natasha Makabilo Laban, Caroline Cleopatra Chisenga, Gibson Sijumbila, Michelo Simuyandi, Roma Chilengi
INTRODUCTION: Deployment of rotavirus vaccines has contributed to significant declines in diarrheal morbidity and mortality globally. Unfortunately, vaccine performance in low-middle income countries (LMICs) is generally lower than in developed countries. The cause for this has been associated with several host and maternal factors including poor water sanitation and hygiene (WASH) status, which are predominant in LMICs. More recently, environmental enteric dysfunction (EED) has specifically been hypothesized to contribute to poor vaccine uptake and response...
2017: PloS One
https://www.readbyqxmd.com/read/29278786/high-throughput-and-accurate-serum-proteome-profiling-by-integrated-sample-preparation-technology-and-single-run-data-independent-mass-spectrometry-analysis
#16
Lin Lin, Jiaxin Zheng, Quan Yu, Wendong Chen, Jinchun Xing, Chenxi Chen, Ruijun Tian
Mass spectrometry (MS)-based serum proteome analysis is extremely challenging due to its high complexity and dynamic range of protein abundances. Developing high throughput and accurate serum proteomic profiling approach capable of analyzing large cohorts is urgently needed for biomarker discovery. Herein, we report a streamlined workflow for fast and accurate proteomic profiling from 1μL of blood serum. The workflow combined an integrated technique for highly sensitive and reproducible sample preparation and a new data-independent acquisition (DIA)-based MS method...
December 23, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29276152/in%C3%A2-vitro-differentiation-of-gata2-and-ly6a-reporter-embryonic-stem-cells-corresponds-to-in%C3%A2-vivo-waves-of-hematopoietic-cell-generation
#17
Mari-Liis Kauts, Carmen Rodriguez-Seoane, Polynikis Kaimakis, Sandra C Mendes, Xabier Cortés-Lavaud, Undine Hill, Elaine Dzierzak
In vivo hematopoietic generation occurs in waves of primitive and definitive cell emergence. Differentiation cultures of pluripotent embryonic stem cells (ESCs) offer an accessible source of hematopoietic cells for blood-related research and therapeutic strategies. However, despite many approaches, it remains a goal to robustly generate hematopoietic progenitor and stem cells (HP/SCs) in vitro from ESCs. This is partly due to the inability to efficiently promote, enrich, and/or molecularly direct hematopoietic emergence...
December 15, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/29273713/ferromagnetic-phase-in-nonequilibrium-quantum-dots
#18
WenJie Hou, YuanDong Wang, JianHua Wei, YiJing Yan
By nonperturbatively solving the nonequilibrium Anderson two-impurity model with the hierarchical equations of motion approach, we report a robust ferromagnetic (FM) phase in series-coupled double quantum dots, which can suppress the antiferromagnetic (AFM) phase and dominate the phase diagram at finite bias and detuning energy in the strongly correlated limit. The FM exchange interaction origins from the passive parallel spin arrangement caused by the Pauli exclusion principle during the electrons transport...
December 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29260371/analysis-of-the-vasculature-by-immunohistochemistry-in-paraffin-embedded-brains
#19
Yann Decker, Andreas Müller, Eszter Németh, Walter J Schulz-Schaeffer, Marc Fatar, Michael D Menger, Yang Liu, Klaus Fassbender
The brain vasculature can be investigated in different ways ranging from in vivo to biochemical analysis. Immunohistochemistry is a simple and powerful technique that can also be applied to archival tissues. However, staining of brain vessels on paraffin sections has been challenging. In this study, we developed an optimized method that can be used in paraffin-embedded mouse and human brain tissues derived from healthy controls and neurological disorders such as Alzheimer's disease. We subsequently showed that this method is fully compatible with the detection of glial cells and key markers of Alzheimer's disease including amyloid beta and phosphorylated Tau protein...
December 19, 2017: Brain Structure & Function
https://www.readbyqxmd.com/read/29258521/long-term-trends-in-supply-and-sustainability-of-the-health-workforce-in-remote-aboriginal-communities-in-the-northern-territory-of-australia
#20
Yuejen Zhao, Deborah J Russell, Steven Guthridge, Mark Ramjan, Michael P Jones, John S Humphreys, Timothy A Carey, John Wakerman
BACKGROUND: International evidence suggests that a key to improving health and attaining more equitable health outcomes for disadvantaged populations is a health system with a strong primary care sector. Longstanding problems with health workforce supply and turnover in remote Aboriginal communities in the Northern Territory (NT), Australia, jeopardise primary care delivery and the effort to overcome the substantial gaps in health outcomes for this population. This research describes temporal changes in workforce supply in government-operated clinics in remote NT communities through a period in which there has been a substantial increase in health funding...
December 19, 2017: BMC Health Services Research
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