keyword
MENU ▼
Read by QxMD icon Read
search

Double robustness

keyword
https://www.readbyqxmd.com/read/29150196/single-stage-urethroplasty-with-buccal-mucosal-inlay-graft-for-stricture-caused-by-balanitis-xerotica-obliterans-in-boys-outcomes-in-the-medium-term
#1
Junaid Ashraf, Alexander Turner, Ramnath Subramaniam
INTRODUCTION: Balanitis xerotica obliterans (BXO) is a common condition that can affect the foreskin, glans, meatus, and urethra, and rarely can also involve urethra leading to stricture. Numerous procedures have been suggested to treat urethral stricture secondary to BXO but these have had variable results. OBJECTIVE: We describe the first prospective study of a single stage buccal mucosal inlay grafting in children with urethral strictures resistant to existing remedies...
October 31, 2017: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/29150004/dynamics-of-indel-profiles-induced-by-various-crispr-cas9-delivery-methods
#2
Michael Kosicki, Sandeep S Rajan, Flaminia C Lorenzetti, Hans H Wandall, Yoshiki Narimatsu, Emmanouil Metzakopian, Eric P Bennett
The introduction of CRISPR/Cas9 gene editing in mammalian cells is a scientific breakthrough, which has greatly affected basic research and gene therapy. The simplicity and general access to CRISPR/Cas9 reagents has in an unprecedented manner "democratized" gene targeting in biomedical research, enabling genetic engineering of any gene in any cell, tissue, organ, and organism. The ability for fast, precise, and efficient profiling of the double-stranded break induced insertions and deletions (indels), mediated by any of the available programmable nucleases, is paramount to any given gene targeting approach...
2017: Progress in Molecular Biology and Translational Science
https://www.readbyqxmd.com/read/29144536/isolation-and-whole-genome-sequencing-of-fetal-cells-from-maternal-blood-towards-the-ultimate-non-invasive-prenatal-testing
#3
Fang Chen, Ping Liu, Ying Gu, Zhu Zhu, Amulya Nanisetti, Zhangzhang Lan, Zhiwei Huang, Sophie Jia Liu, Xiongbin Kang, Yuqing Deng, Liqiong Luo, Dan Jiang, Yong Qiu, Jianchang Pan, Jun Xia, Ken Xiong, Chao Liu, Lin Xie, Qianyu Shi, Jing Li, Xiuqing Zhang, Wei Wang, Snezana Drmanac, Hui Jiang, Radoje Drmanac, Xun Xu
OBJECTIVE: To develop a methodology of isolating fetal cells from maternal blood and use deep sequencing demonstrating the promise for more complete and accurate genetic screening compared to other non-invasive prenatal testing (NIPT). METHODS: Here in this study, we developed a double negative selection (DNS) procedure to unbiasedly enrich fetal cells. After validated by short tandem repeat (STR), the isolated CFCs were subjected to deep whole genome sequencing analysis (WGS)...
November 16, 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/29143340/k2p-task-2-and-kcnq1-kcne3-k-channels-are-major-players-contributing-to-intestinal-anion-and-fluid-secretion
#4
Francisca Julio-Kalajzić, Sandra Villanueva, Johanna Burgos, Margarita Ojeda, L Pablo Cid, Thomas J Jentsch, Francisco V Sepúlveda
Anion and fluid secretion across the intestinal epithelium, a process altered in cystic fibrosis and secretory diarrhoea, is mediated by cAMP-activated CFTR Cl(-) channels and requires the simultaneous activity of basolateral K(+) channels to maintain cellular ionic homeostasis and membrane potential. This function is fulfilled by the cAMP-activated K(+) channel formed by the association of pore-forming KCNQ1 with its obligatory KCNE3 β-subunit. Studies using mice show sizable cAMP-activated intestinal anion secretion in the absence of either KCNQ1 or KCNE3 suggesting that an alternative K(+) conductance must compensate for the loss of KCNQ1/KCNE3 activity...
November 16, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/29142497/expression-of-ccl2-and-its-receptor-in-activation-and-migration-of-microglia-and-monocytes-induced-by-photoreceptor-apoptosis
#5
ChaoYi Feng, Xin Wang, TianJin Liu, Meng Zhang, GeZhi Xu, YingQin Ni
Purpose: To explore the effect of the CCL2 and CCR2 system on the activation and migration of microglia and monocytes in light-induced photoreceptor apoptosis. Methods: At 1 day, 3 days, 7 days, and 14 days after light exposure, OX42 and ED1 immunostaining were used to label the activation and migration of microglia and monocytes. Double immunostaining of CCL2 with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), OX42, or glial fibrillary acidic protein (GFAP) was applied to explore the relationships among CCL2, apoptotic photoreceptors, activated microglia and monocytes, and macroglial cells (Müller cells and astrocytes)...
2017: Molecular Vision
https://www.readbyqxmd.com/read/29142122/adaptive-immune-responses-following-senecavirus-a-infection-in-pigs
#6
Mayara F Maggioli, Steve Lawson, Marcelo de Lima, Lok R Joshi, Tatiane C Faccin, Fernando V Bauermann, Diego G Diel
Senecavirus A (SVA), an emerging picornavirus of swine, causes vesicular disease (VD) that is clinically indistinguishable from foot-and-mouth disease (FMD) in pigs. Many aspects of SVA interactions with the host and the host immune responses to infection, however, remain unknown. In the present study, humoral and cellular immune responses to SVA were evaluated following infection in pigs. We show that SVA infection elicited an early and robust neutralizing antibody (NA) response, which coincided with and was strongly correlated with VP2- and VP3-specific IgM responses...
November 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29141988/genotyping-by-sequencing-in-almond-snp-discovery-linkage-mapping-and-marker-design
#7
Shashi N Goonetilleke, Timothy J March, Michelle G Wirthensohn, Pere Arús, Amanda R Walker, Diane E Mather
In crop plant genetics, linkage maps provide the basis for the mapping of loci that affect important traits and for the selection of markers to be applied in crop improvement. In outcrossing species such as almond [Prunus dulcis (Mill.) D.A. Webb], application of a double pseudo-testcross mapping approach to the F1 progeny of a bi-parental cross leads to the construction of a linkage map for each parent. Here, we report on the application of genotyping-by-sequencing to discover and map single-nucleotide polymorphisms in the almond cultivars 'Nonpareil' and 'Lauranne'...
November 15, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29141439/double-cross-polarization-for-the-indirect-detection-of-nitrogen-14-nuclei-in-magic-angle-spinning-nmr-spectroscopy
#8
Diego Carnevale, Xiao Ji, Geoffrey Bodenhausen
Nitrogen-14 NMR spectra at fast magic-angle spinning rates can be acquired indirectly by means of two-dimensional techniques based on double cross polarization transfer (1)H → (14)N →(1)H. Experimental evidence is given for polycrystalline samples of glycine, l-histidine, and the dipeptide Ala-Gly. Either one-bond or long-range correlations can be favored by choosing the length of the cross polarization contact pulses. Longer contact pulses allow the detection of unprotonated nitrogen sites. In contrast to earlier methods that exploited second-order quadrupolar/dipolar cross-terms, cross polarization operates in the manner of the method of Hartmann and Hahn, even for (14)N quadrupolar couplings up to 4 MHz...
November 14, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29141219/preventive-inhibition-of-liver-tumorigenesis-by-systemic-activation-of-innate-immune-functions
#9
Jin Lee, Rui Liao, Gaowei Wang, Bi-Huei Yang, Xiaolin Luo, Nissi M Varki, Shuang-Jian Qiu, Bing Ren, Wenxian Fu, Gen-Sheng Feng
Liver cancer has become the second most deadly malignant disease, with no efficient targeted or immune therapeutic agents available yet. While dissecting the roles of cytoplasmic signaling molecules in hepatocarcinogenesis using an inducible mouse gene targeting system, Mx1-cre, we identified a potent liver tumor-inhibitory effect of synthetic double-stranded RNA (dsRNA), polyinosinic-polycytidylic acid (pIC), an inducer of the Mx1-cre system. Injection of pIC at the pre-cancer stage robustly suppressed liver tumorigenesis either induced by chemical carcinogens or by Pten loss and associated hepatosteatosis...
November 14, 2017: Cell Reports
https://www.readbyqxmd.com/read/29139200/allosteric-mechanism-of-quinoline-inhibitors-for-hiv-rt-associated-rnase-with-md-simulation-and-dynamics-fluctuation-network
#10
Yi Cai, Hao Liu, Haifeng Chen
The human immunodeficiency virus (HIV) is a retrovirus which infects T lymphocyte of human body and causes Immunodeficiency. Reverse transcriptase inhibitors (RTIs) can inhibit some functions of RT, preventing virus synthesis (double-stranded DNA), so that HIV virus replication can be reduced. Experimental results indicate a series of benzimidazole-based inhibitors which target HIV RT-associate RNase to inhibit the reverse transcription of HIV virus. However, the allosteric mechanism is still unclear. Here, molecular dynamics simulations and dynamics fluctuation network analysis were used to reveal the binding mode between the inhibitors and RT-associate RNase...
November 15, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/29136592/human-sirtuin-3-sirt3-deacetylates-histone-h3-lysine-56-to-promote-nonhomologous-end-joining-repair
#11
Amrita Sengupta, Devyani Haldar
Human sirtuin 3 (SIRT3) is a conserved NAD(+) dependent deacetylase, which functions in important cellular processes including transcription, metabolism, oxidative stress response. It is a robust mitochondrial deacetylase; however, few studies have indicated its nuclear functions. Here we report interaction of SIRT3 with core histones and identified acetylated histone H3 lysine 56 (H3K56ac) as its novel substrate, in addition to known substrates acetylated H4K16 and H3K9. Further, we showed in response to DNA damage SIRT3 localizes to the repair foci colocalizing with γH2AX and nonhomologous end joining (NHEJ) marker p53-binding protein 1 (53BP1)...
November 8, 2017: DNA Repair
https://www.readbyqxmd.com/read/29135956/an-improved-aerial-target-localization-method-with-a-single-vector-sensor
#12
Anbang Zhao, Xuejie Bi, Juan Hui, Caigao Zeng, Lin Ma
This paper focuses on the problems encountered in the actual data processing with the use of the existing aerial target localization methods, analyzes the causes of the problems, and proposes an improved algorithm. Through the processing of the sea experiment data, it is found that the existing algorithms have higher requirements for the accuracy of the angle estimation. The improved algorithm reduces the requirements of the angle estimation accuracy and obtains the robust estimation results. The closest distance matching estimation algorithm and the horizontal distance estimation compensation algorithm are proposed...
November 14, 2017: Sensors
https://www.readbyqxmd.com/read/29135224/radiation-responsive-esculin-derived-molecular-gels-as-signal-enhancers-for-optical-imaging
#13
Julian R Silverman, Qize Zhang, Nabendu B Pramanik, Malick Samateh, Travis M Shaffer, Sai Sateesh Sagiri, Jan Grimm, George John
Recent interest in detecting visible photons that emanate from interactions of ionizing radiation with matter have spurred the development of multifunctional materials that amplify this optical signal from radiotracers. Tailored stimuli-responsive systems may be paired with diagnostic radionuclides to improving surgical guidance and aid in detecting therapeutic radionuclides otherwise difficult to image with conventional nuclear medicine approaches. Since light emanating from these interactions is typically low in intensity and blue-weighted (i...
November 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29132300/plant-mediated-rnai-silences-midgut-expressed-genes-in-congeneric-lepidopteran-insects-in-nature
#14
Spoorthi Poreddy, Jiancai Li, Ian T Baldwin
BACKGROUND: Plant-mediated RNAi (PMRi) silencing of insect genes has enormous potential for crop protection, but whether it works robustly under field conditions, particularly with lepidopteran pests, remains controversial. Wild tobacco Nicotiana attenuata and cultivated tobacco (N. tabacum) (Solanaceae) is attacked by two closely related specialist herbivores Manduca sexta and M. quinquemaculata (Lepidoptera, Sphingidae). When M. sexta larvae attack transgenic N. attenuata plants expressing double-stranded RNA(dsRNA) targeting M...
November 13, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29124562/video-based-heart-rate-monitoring-across-a-range-of-skin-pigmentations-during-an-acute-hypoxic-challenge
#15
Paul S Addison, Dominique Jacquel, David M H Foo, Ulf R Borg
The robust monitoring of heart rate from the video-photoplethysmogram (video-PPG) during challenging conditions requires new analysis techniques. The work reported here extends current research in this area by applying a motion tolerant algorithm to extract high quality video-PPGs from a cohort of subjects undergoing marked heart rate changes during a hypoxic challenge, and exhibiting a full range of skin pigmentation types. High uptimes in reported video-based heart rate (HRvid) were targeted, while retaining high accuracy in the results...
November 9, 2017: Journal of Clinical Monitoring and Computing
https://www.readbyqxmd.com/read/29117179/a-mutation-in-the-viral-sensor-2-5-oligoadenylate-synthetase-2-causes-failure-of-lactation
#16
Samantha R Oakes, David Gallego-Ortega, Prudence M Stanford, Simon Junankar, Wendy Wing Yee Au, Zoya Kikhtyak, Anita von Korff, Claudio M Sergio, Andrew M K Law, Lesley E Castillo, Stephanie L Allerdice, Adelaide I J Young, Catherine Piggin, Belinda Whittle, Edward Bertram, Matthew J Naylor, Daniel L Roden, Jesse Donovan, Alexei Korennykh, Christopher C Goodnow, Moira K O'Bryan, Christopher J Ormandy
We identified a non-synonymous mutation in Oas2 (I405N), a sensor of viral double-stranded RNA, from an ENU-mutagenesis screen designed to discover new genes involved in mammary development. The mutation caused post-partum failure of lactation in healthy mice with otherwise normally developed mammary glands, characterized by greatly reduced milk protein synthesis coupled with epithelial cell death, inhibition of proliferation and a robust interferon response. Expression of mutant but not wild type Oas2 in cultured HC-11 or T47D mammary cells recapitulated the phenotypic and transcriptional effects observed in the mouse...
November 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29116743/a-3d-printable-and-mechanically-robust-hydrogel-based-on-alginate-and-graphene-oxide
#17
Sijun Liu, Anil Kumar Bastola, Lin Li
Sodium alginate (SA) was used for the first time to noncovalently functionalize amino-graphene oxide (aGO) to produce the SA-functionalized GO, A-aGO. A-aGO was then filled into a double-network (DN) hydrogel consisting of an alginate network (SA) and a polyacrylamide (PAAm) network. Before UV curing, A-aGO was able to provide the SA/PAAm DN hydrogel with a remarkable thixotropic property, which is desirable for 3D printing. Thus, the A-aGO-filled DN hydrogel could be nicely used as an "ink" of a 3D printer to print complicated 3D structures with a high stackability and high shape fidelity...
November 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29114950/inorganic-organic-double-network-gels-containing-ionic-liquids
#18
Eiji Kamio, Tomoki Yasui, Yu Iida, Jian Ping Gong, Hideto Matsuyama
Highly robust ion gels, termed double-network (DN) ion gels, composed of inorganic/organic interpenetrating networks and a large amount of ionic liquids (ILs), are fabricated. The DN ion gels with an 80 wt% IL content show extraordinarily high mechanical strength: more than 28 MPa of compressive fracture stress. In the DN ion gel preparation, a brittle inorganic network of physically bonded silica nanoparticles and a ductile organic network of polydimethylacrylamide (PDMAAm) are formed in the IL. Because of the different reaction mechanisms of the inorganic/organic networks, the DN ion gels can be formed by an easy and free-shapeable one-pot synthesis...
November 8, 2017: Advanced Materials
https://www.readbyqxmd.com/read/29114595/use-of-regional-nodal-irradiation-and-its-association-with-survival-for-women-with-high-risk-early-stage-breast-cancer-a-national-cancer-database-analysis
#19
Amy C Moreno, Yan Heather Lin, Isabelle Bedrosian, Yu Shen, Michael C Stauder, Benjamin D Smith, Thomas A Buchholz, Gildy V Babiera, Wendy A Woodward, Simona F Shaitelman
Purpose: The role of regional nodal irradiation (RNI) for patients with breast cancer remains controversial, particularly on the basis of nodal involvement. Using the National Cancer Database, we aimed to validate published data on whether expanding treatment fields from whole-breast irradiation (WBI) to encompass the regional nodes (WBI+RNI) affected overall survival (OS) for patients with node-positive (pN1-3) or high-risk node-negative (pN0) breast cancer treated with breast-conserving surgery and adjuvant chemotherapy...
July 2017: Advances in Radiation Oncology
https://www.readbyqxmd.com/read/29112893/natural-product-%C3%AE-thujaplicin-inhibits-homologous-recombination-repair-and-sensitizes-cancer-cells-to-radiation-therapy
#20
Lihong Zhang, Yang Peng, Ivan P Uray, Jianfeng Shen, Lulu Wang, Xiangdong Peng, Powel H Brown, Wei Tu, Guang Peng
Investigation of natural products is an attractive strategy to identify novel compounds for cancer prevention and treatment. Numerous studies have shown the efficacy and safety of natural products, and they have been widely used as alternative treatments for a wide range of illnesses, including cancers. However, it remains unknown whether natural products affect homologous recombination (HR)-mediated DNA repair and whether these compounds can be used as sensitizers with minimal toxicity to improve patients' responses to radiation therapy, a mainstay of treatment for many human cancers...
October 24, 2017: DNA Repair
keyword
keyword
55429
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"