Kiyoshi Kikuchi, Kentaro Setoyama, Eiichiro Tanaka, Shotaro Otsuka, Takuto Terashi, Kazuki Nakanishi, Seiya Takada, Harutoshi Sakakima, Sumate Ampawong, Ko-Ichi Kawahara, Tomoka Nagasato, Kazuya Hosokawa, Yoichiro Harada, Mika Yamamoto, Chinatsu Kamikokuryo, Ryoji Kiyama, Motohiro Morioka, Takashi Ito, Ikuro Maruyama, Salunya Tancharoen
Uric acid (UA) therapy may prevent early ischemic worsening after acute stroke in thrombolysis patients. The aim of this study was to examine the influence of UA on the thrombolytic efficacy of alteplase in human blood samples by measuring thrombolysis under flow conditions using a newly developed microchip-based flow-chamber assay. Human blood samples from healthy volunteers were exposed to UA, alteplase, or a combination of UA and alteplase. Whole blood and platelet-rich plasma were perfused over a collagen- and thromboplastin-coated microchip, and capillary occlusion was monitored with a video microscope and flow-pressure sensor...
October 26, 2018: Scientific Reports