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https://www.readbyqxmd.com/read/29166726/-interaction-between-trpc1-and-stim1-in-calcium-sensing-receptor-mediated-calcium-influx-and-nitric-oxide-production-in-human-umbilical-vein-endothelial-cells
#1
L M Wang, H Zhong, N Tang, L J Pang, C J Zhang, F He
Objective: To investigate the interaction of Ca(2+) protein TRPC1 and STIM1 in extracellular Ca(2+) -sensing receptor (CaR)-induced extracellular Ca(2+) influx and the production of nitric oxide (NO). Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and incubated with CaR agonist spermine (activating store-operates cation channels (SOC) and receptor-operated channels (ROC)), CaR negative allosteric modulator Calhex231 (blocking SOC, activating ROC) and ROC analogue TPA (activating ROC, blocking SOC), protein kinase C (PKC) inhibitor Ro31-8220, PKCs and PKCμ inhibitor Go6967(activate SOC, blocking ROC), respectively...
November 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/29166642/effect-of-calcium-infiltrated-hydroxyapatite-scaffolds-on-the-hematopoietic-fate-of-human-umbilical-vein-endothelial-cells
#2
Qinghao Zhang, Jörg C Gerlach, Eva Schmelzer, Ian Nettleship
Foamed hydroxyapatite offers a three-dimensional scaffold for the development of bone constructs, mimicking perfectly the in vivo bone structure. In vivo, calcium release at the surface is assumed to provide a locally increased gradient supporting the maintenance of the hematopoietic stem cells niche. We fabricated hydroxyapatite scaffolds with high surface calcium concentration by infiltration, and used human umbilical vein endothelial cells (HUVECs) as a model to study the effects on hematopoietic lineage direction...
November 23, 2017: Journal of Vascular Research
https://www.readbyqxmd.com/read/29165229/na%C3%A3-ve-t-cells-are-activated-by-autologous-hcmv-infected-endothelial-cells-through-nkg2d-and-can-control-hcmv-transmission-in-vitro
#3
Narmadha Subramanian, Zeguang Wu, Frank Reister, Kerstin Laib Sampaio, Giada Frascaroli, Luka Cicin-Sain, Thomas Mertens
Apart from classical antigen-presenting cells (APCs) like dendritic cells and macrophages, there are semiprofessional APCs such as endothelial cells (ECs) and Langerhans' cells. Human cytomegalovirus (HCMV) infects a wide range of cell types including the ECs which are involved in the trafficking and homing of T cells. By investigating the interaction of naïve T cells obtained from HCMV-seronegative umbilical cord blood with autologous HCMV-infected human umbilical vein ECs (HUVECs), we could show that the activation of naïve T cells occurred after 1 day of peripheral blood mononuclear cell (PBMC) exposure to HCMV-infected HUVECs...
November 21, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/29165102/radio-protective-effect-and-mechanism-of-4-acetamido-2-2-6-6-tetramethylpiperidin-1-oxyl-in-huvec-cells
#4
Feng Wang, Peng Gao, Ling Guo, Ping Meng, Yuexing Fan, Yongbin Chen, Yanyun Lin, Guozhen Guo, Guirong Ding, Haibo Wang
OBJECTIVES: To search for more effective radiation protectors with minimal toxicity, a water-soluble nitroxides Acetamido-Tempol (AA-Tempol) was evaluated for potential radioprotective properties in HUVEC cells (Human Umbilical Vein Endothelial cell line). METHODS: To study the anti-radiation effect of AA-Tempol in cell culture, the viability of irradiated HUVEC cells using a clonogenic survival assay was examined. The anti-apoptosis effects of AA-Tempol using Annexin V/propidium iodide staining in a flow cytometry assay was also evaluated...
March 24, 2017: Environmental Health and Preventive Medicine
https://www.readbyqxmd.com/read/29164102/anti-tumor-and-anti-angiogenic-ergosterols-from-ganoderma-lucidum
#5
Shaodan Chen, Tianqiao Yong, Yifang Zhang, Jiyan Su, Chunwei Jiao, Yizhen Xie
This study was carried out to isolate chemical constituents from the lipid enriched fraction of Ganoderma lucidum extract and to evaluate their anti-proliferative effect on tumor cells and human umbilical vein endothelial cells (HUVECs). Ergosterol derivatives (1-14) were isolated and purified from the lipid enriched fraction of G. lucidum. Their chemical structures were established by spectroscopic analyses or by comparison of mass and NMR spectral data with those reported previously. Amongst, compound 1 was purified and identified as a new one...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/29162452/dt-13-ameliorates-tnf-%C3%AE-induced-nitric-oxide-production-in-the-endothelium-in-vivo-and-in-vitro
#6
Ruiping Fan, Yuwei Han, Han Han, Zhengdong Chen, Boyang Yu, Junping Kou, Yuanyuan Zhang
The steroidal saponin DT-13 (25(R,S)-ruscogenin-1-O-[β-d-glucopyranosyl-(1 → 2)][β-d-xylopyranosyl-(1 → 3)]-β-d-fucopyranoside), one of the major active compounds of the herb Liriope muscari (Decne.), exhibits significant anti-inflammatory, anti-tumour and cardioprotective effects. This study aimed to explore the protective effect of DT-13 on endothelium through regulating of nitric oxide production induced by TNF-α. The results demonstrated that DT-13 inhibits inflammatory cell infiltration and thus plays a protective effect, as shown by H&E staining and immunohistochemical staining...
November 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29161413/nrf2-regulates-endothelial-glycolysis-and-proliferation-with-mir-93-and-mediates-the-effects-of-oxidized-phospholipids-on-endothelial-activation
#7
Suvi M Kuosmanen, Emilia Kansanen, Minna U Kaikkonen, Virve Sihvola, Kati Pulkkinen, Henna-Kaisa Jyrkkänen, Pauli Tuoresmäki, Juha Hartikainen, Mikko Hippeläinen, Hannu Kokki, Pasi Tavi, Sami Heikkinen, Anna-Liisa Levonen
Phospholipids, such as 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (PAPC), are the major components of cell membranes. Their exposure to reactive oxygen species creates oxidized phospholipids, which predispose to the development of chronic inflammatory diseases and metabolic disorders through endothelial activation and dysfunction. Although the effects of oxidized PAPC (oxPAPC) on endothelial cells have been previously studied, the underlying molecular mechanisms evoking biological responses remain largely unknown...
November 17, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29158813/vascularized-tissue-engineered-model-for-studying-drug-resistance-in-neuroblastoma
#8
A Villasante, K Sakaguchi, J Kim, N K Cheung, M Nakayama, H Parsa, T Okano, T Shimizu, G Vunjak-Novakovic
Neuroblastoma is a vascularized pediatric tumor derived from neural crest stem cells that displays vasculogenic mimicry and can express a number of stemness markers, such as SOX2 and NANOG. Tumor relapse is the major cause of succumbing to this disease, and properties attributed to cancer stem-like cells (CSLC), such as drug-resistance and cell plasticity, seem to be the key mechanisms. However, the lack of controllable models that recapitulate the features of human neuroblastoma limits our understanding of the process and impedes the development of new therapies...
2017: Theranostics
https://www.readbyqxmd.com/read/29158804/heparanase-contributes-to-trans-endothelial-migration-of-hepatocellular-carcinoma-cells
#9
Xiaopeng Chen, Wen Jiang, Chaofu Yue, Wenjun Zhang, Chaogang Tong, Dafei Dai, Bin Cheng, Chen Huang, Linming Lu
The overall outcome of patients with hepatocellular carcinoma (HCC) is still very poor due to its high metastasis and recurrence rate. During metastasis, trans-endothelial migration (TEM) of HCC cells is a key step. Heparanase (HPSE) is an endo-beta-glucuronidase and exerts prometastatic properties for normal and tumor-derived cells. However, it is remains unclear that HPSE contributes to TEM of HCC cells. In this study, human umbilical vein endothelial cells-C (HUVEC-C) was used to simulate vascular endothelial cells (VECs), and the HCCLM3 cells with high HPSE expression were chosen and used for in vitro TEM assay and in vivo experiment...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29157819/pro-angiogenic-effects-of-ilexsaponin-a1-on-human-umbilical-vein-endothelial-cells-in-vitro-and-zebrafish-in-vivo
#10
Jingjing Li, Jinming Zhang, Liang Zou, Simon Ming-Yuen Lee, Cui Yang, Sai-Wang Seto, George Pak-Heng Leung
BACKGROUND: Ilexsaponin A1 is the major bioactive ingredient of Ilex pubescens Hook. et Arn. This plant has been conventionally used in Traditional Chinese Medicine for the treatment of cardiovascular diseases including stroke, coronary arterial disease, and peripheral vascular diseases. PURPOSE: To investigate the pro-angiogenic effect of Ilexsaponin A1 and its mechanism of action. STUDY DESIGN: Human umbilical vein endothelial cells (HUVECs) and transgenic zebrafish Tg(fli1:EGFP) were employed as an in vitro and in vivo model respectively...
December 1, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29156700/linc00152-suppresses-apoptosis-and-promotes-migration-by-sponging-mir-4767-in-vascular-endothelial-cells
#11
Wei Teng, Chunguang Qiu, Zhaohui He, Guoliang Wang, Yongliang Xue, Xuezhi Hui
Dysfunction of vascular endothelial cells (VECs), such as increased apoptosis and diminished migration, is closely connected with most cardiovascular diseases and angiogenesis-related events. LncRNAs have been involved in regulation of many pathological processes, but their roles in vascular endothelial function are hardly underreported. Here, we explore the role of a intergenic lncRNA named linc00152 in the apoptosis and migration of VECs. We found that linc00152 was downregulated in human umbilical vein VECs (HUVECs) in a dose- and time-dependent manner following treatment with oxLDL, which is a typical proinflammatory factor in the initiation and progression of vascular endothelial dysfunction...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29155953/max-mutations-in-endometrial-cancer-clinicopathologic-associations-and-recurrent-max-p-his28arg-functional-characterization
#12
Christopher J Walker, Craig M Rush, Paola Dama, Matthew J O'Hern, Casey M Cosgrove, Jessica L Gillespie, Roman A Zingarelli, Blair Smith, Maggie E Stein, David G Mutch, Reena Shakya, Chia-Wen Chang, Karuppaiyah Selvendiran, Jonathan W Song, David E Cohn, Paul J Goodfellow
Background: Genomic studies have revealed that multiple genes are mutated at varying frequency in endometrial cancer (EC); however, the relevance of many of these mutations is poorly understood. An EC-specific recurrent mutation in the MAX transcription factor p.His28Arg was recently discovered. We sought to assess the functional consequences of this hotspot mutation and determine its association with cancer-relevant phenotypes. Methods: MAX was sequenced in 509 endometrioid ECs, and associations between mutation status and clinicopathologic features were assessed...
November 15, 2017: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/29155213/human-umbilical-vein-endothelium-derived-exosomes-play-a-role-in-foetoplacental-endothelial-dysfunction-in-gestational-diabetes-mellitus
#13
Tamara Sáez, Rocío Salsoso, Andrea Leiva, Fernando Toledo, Paul de Vos, Marijke Faas, Luis Sobrevia
Gestational diabetes mellitus (GDM) characterizes by foetoplacental endothelial dysfunction. Human umbilical vein endothelial cells (HUVECs) from women with GDM show increased L-arginine transport via the human cationic amino acid transporter 1 (hCAT-1). Moreover, expression of endothelial nitric oxide synthase (eNOS) and nitric oxide synthesis are increased. Exosomes are increased in maternal plasma from GDM. We evaluated the role of foetoplacental endothelial exosomes on endothelial dysfunction in GDM. Exosomes were isolated from HUVECs from normal (ExN) and GDM (ExGDM) pregnancies...
November 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29155057/effects-of-different-hypoxia-degrees-on-endothelial-cell-cultures-time-course-study
#14
Ioana Baldea, Ioana Teacoe, Diana Elena Olteanu, Cristina Vaida-Voievod, Andra Clichici, Alexandru Sirbu, Gabriela Adriana Filip, Simona Clichici
INTRODUCTION: Exposure of the endothelial cells to hypoxia, the decrease in oxygen supply can trigger an endothelial response. This response is involved in inflammatory diseases, tumorigenesis, and also with the micro vascular damage associated with aging. The aim of our study was to determine the hypoxia/re-oxygenation induced response in vitro, using human umbilical vein endothelial cells (HUVEC) cultures, at different time points with focus on cell viability, apoptosis oxidative stress and angiogenesis stimulation...
November 15, 2017: Mechanisms of Ageing and Development
https://www.readbyqxmd.com/read/29154990/exosomes-derived-from-human-umbilical-vein-endothelial-cells-promote-neural-stem-cell-expansion-while-maintain-their-stemness-in-culture
#15
Yi-Zhe Zhang, Fan Liu, Chang-Geng Song, Xiu-Li Cao, Yu-Fei Zhang, Hai-Ning Wu, Chen-Jun Guo, Yong-Qiang Li, Qi-Jun Zheng, Min-Hua Zheng, Hua Han
The neural stem cell (NSC) niche in subventricular zone (SVZ) of adult mammalian brain contains dense vascular plexus, where endothelial cells (ECs) regulate NSCs by releasing plenty of angiocrine factors. However, the role of ECs-derived exosomes, a novel type of mediators of intercellular communications, in the regulation of NSCs remains unclear. In the current study, primary NSCs isolated from embryonic mouse brains form more neurospheres when cultured in the presence of human umbilical vein endothelial cells (HUVECs)...
November 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29154827/secretogranin-iii-promotes-angiogenesis-through-mek-erk-signaling-pathway
#16
Fen Tang, Mario Thiego F Pacheco, Ping Chen, Dan Liang, Wei Li
Secretogranin III (Scg3) was recently discovered as the first highly diabetic retinopathy-associated angiogenic factor, and its neutralizing antibody alleviated the disease with high efficacy in diabetic mice. Investigation of its molecular mechanisms will facilitate the translation of this novel therapy. Scg3 was reported to induce the phosphorylation of mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK). Here we characterized the importance of MEK/ERK activation to Scg3 angiogenic activity...
November 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29151977/pfkfb3-promotes-proliferation-migration-and-angiogenesis-in-nasopharyngeal-carcinoma
#17
Miao Gu, Li Li, Zhenxin Zhang, Jing Chen, Wei Zhang, Jie Zhang, Liang Han, Mingming Tang, Bo You, Qicheng Zhang, Yiwen You
Nasopharyngeal carcinoma (NPC) is a squamous epithelial cancer, arising from the nasopharynx epithelium. It has high morbidity and mortality. PFKFB3 as a glycolytic activator has been implicated in the progression of multiple types of tumor. PFKFB3 can be contributed to the progression and metastasis of cancer. However, whether PFKFB3 is associated with the progression of NPC remains unknown. We postulated that PFKFB3 promotes proliferation, migration and angiogenesis in nasopharyngeal carcinoma. In this study, we found that PFKFB3 was significantly up-regulated in NPC tissues and cell lines compared with normal control...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29149082/the-biological-properties-of-ogi-surfaces-positively-act-on-osteogenic-and-angiogenic-commitment-of-mesenchymal-stem-cells
#18
Paolo Ghensi, Eriberto Bressan, Chiara Gardin, Letizia Ferroni, Maria Costanza Soldini, Federico Mandelli, Claudio Soldini, Barbara Zavan
Osteogenesis process displays a fundamental role during dental implant osteointegration. In the present work, we studied the influence of Osteon Growth Induction (OGI) surface properties on the angiogenic and osteogenic behaviors of Mesenchymal Stem cells (MSC). MSC derived from dental pulp and HUVEC (Human Umbilical Vein Endothelial Cells) were grown in on OGI titanium surfaces, and cell proliferation and DNA synthesis were evaluated by MTT [3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide] test and DNA quantification...
November 17, 2017: Materials
https://www.readbyqxmd.com/read/29148433/a-flow-bioreactor-system-compatible-with-real-time-two-photon-fluorescence-lifetime-imaging-microscopy
#19
Nian Shen, Julia A Riedl, Daniel A Carvajal-Berrio, Zackary Davis, Michael G Monaghan, Shannon Lee Layland, Svenja Hinderer, Katja Schenke-Layland
Bioreactors are essential cell and tissue culture tools that allow the introduction of biophysical signals into in vitro cultures. One major limitation is the need to interrupt experiments and sacrifice samples at certain time points for analyses. To address this issue, we designed a bioreactor that combines high-resolution contact-free imaging and continuous flow in a closed system that is compatible with various types of microscopes. The high-throughput fluid flow bioreactor was combined with two-photon fluorescence lifetime imaging microscopy (2P-FLIM) and validated...
November 17, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/29143455/understanding-the-functional-role-of-microrna-214-3p-in-atherosclerosis-for-the-identification-of-novel-targeted-therapies-to-prevent-or-reverse-endothelial-cell-dysfunction-and-stimulate-autophagy
#20
EDITORIAL
Adriana Georgescu
The original article by Jing Wang and collaborators, entitled "MicroRNA-214-3p: A Link between Autophagy and Endothelial Cell Dysfunction in Atherosclerosis", brings new data concerning molecular mechanisms linking decreased autophagy to endothelial dysfunction in atherosclerosis. In this issue of Acta Physiologica, the authors concluded that microRNA-214-3p (miR-214-3p) controls oxidized low-density lipoprotein (ox-LDL)-induced autophagy in human umbilical vein endothelial cells (HUVECs) by regulating intracellular levels of autophagy-related protein 5 (ATG5), and may have a relevant role in the pathogenesis of atherosclerosis...
November 16, 2017: Acta Physiologica
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