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https://www.readbyqxmd.com/read/29782791/a-method-for-preparation-of-an-internal-layer-of-artificial-vascular-graft-co-modified-with-salvianolic-acid-b-and-heparin
#1
Haizhu Kuang, Yao Wang, Junfeng Hu, Chunsheng Wang, Shuyang Lu, Xiumei Mo
Studies have shown that traditional Chinese medicine Salvianolic acid B (SAB) can promote the proliferation and migration of endothelial cells. Heparin has a good anticoagulant effect. The inner layer of artificial vascular graft is fabricated by coaxial electrospinning method, and loaded with heparin and SAB. The release of heparin and SAB was sustained for almost 30 days, and SAB without initial burst release. Furthermore, the combined effect of SAB and heparin contributed to promoted Human Umbilical Vein Endothelial Cells (HUVECs) growth and improved blood compatibility of graft...
May 21, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29781582/sphingosine-1-phosphate-receptor-1-signaling-maintains-endothelial-cell-barrier-function-and-protects-against-immune-complex-induced-vascular-injury
#2
Nathalie Burg, Steven Swendeman, Stefan Worgall, Timothy Hla, Jane E Salmon
OBJECTIVE: Immune complex (IC) deposition activates neutrophils (PMN), increases vascular permeability and leads to organ damage in SLE and RA. The bioactive lipid sphingosine-1-phosphate (S1P), acting via S1P receptor 1 (S1P1 ), is a key regulator of endothelial cell (EC) barrier function. We hypothesized that augmenting EC integrity via S1P1 signaling would attenuate inflammatory injury mediated by ICs. METHODS: In vitro barrier function was assessed in human umbilical vein endothelial cells (HUVECs) by Electric Cell-substrate Impedance Sensing (ECIS)...
May 21, 2018: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/29777728/testosterone-induced-downregulation-of-migration-and-proliferation-in-human-umbilical-vein-endothelial-cells-by-androgen-receptor-dependent-and-independent-mechanisms
#3
Aulona Gaba, Mario Mairhofer, Zyhdi Zhegu, Nadja Leditznig, Ladislaus Szabo, Walter Tschugguel, Christian Schneeberger, Iveta Yotova
Recent research has emphasized the potential unfavorable effects of declining testosterone (T) levels in men and the putative beneficial effect of androgen therapy in select women. Some controversy surrounding the mechanism of action and the effects of T on endothelium remains. In this study, we evaluated the mechanism of T action on pooled primary Human Umbilical Vein Endothelial Cells (HUVEC) of mixed gender by focusing on two important processes, proliferation and migration. In our in vitro model system, we found that only the supra-physiological dose of T affected these two processes irrespective of the ratio of male to female cells in the pools...
May 16, 2018: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29775893/puerarin-prevents-vascular-endothelial-injury-through-suppression-of-nf-%C3%AE%C2%BAb-activation-in-lps-challenged-human-umbilical-vein-endothelial-cells
#4
Hua-Fei Deng, Sha Wang, Lian Li, Qin Zhou, Wan-Bei Guo, Xiao-Li Wang, Mei-Dong Liu, Ke Liu, Xian-Zhong Xiao
OBJECTIVE: In the present study, we aimed to explore the effects of puerarin on vascular endothelial cell injury induced by lipopolysaccharide (LPS) and its underlying mechanisms. METHODS: The cell viability and morphological changes were assessed using the cell counting kit-8 (CCK-8) assay and 4´,6-diamidino-2-phenylindole (DAPI) staining, respectively. The levels of tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), monocyte/macrophage chemotactic protein-1 (MCP-1), IL-8, intercellular cell adhesion molecule-1 (ICAM-1), thrombomodulin (TM) and plasminogen activator inhibitor-1 (PAI-1) in cell culture supernatant were determined by the enzyme-linked immunosorbent assay (ELISA)...
May 15, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29774068/platelet-camouflaged-nanococktail-simultaneous-inhibition-of-drug-resistant-tumor-growth-and-metastasis-via-a-cancer-cells-and-tumor-vasculature-dual-targeting-strategy
#5
Lijia Jing, Haijing Qu, Dongqi Wu, Chaojian Zhu, Yongbo Yang, Xing Jin, Jian Zheng, Xiangsheng Shi, Xiufeng Yan, Yang Wang
Multidrug resistance (MDR) poses a great challenge to cancer therapy. It is difficult to inhibit the growth of MDR cancer due to its chemoresistance. Furthermore, MDR cancers are more likely to metastasize, causing a high mortality among cancer patients. In this study, a nanomedicine RGD-NPVs@MNPs/DOX was developed by encapsulating melanin nanoparticles (MNPs) and doxorubicin (DOX) inside RGD peptide (c(RGDyC))-modified nanoscale platelet vesicles (RGD-NPVs) to efficiently inhibit the growth and metastasis of drug-resistant tumors via a cancer cells and tumor vasculature dual-targeting strategy...
2018: Theranostics
https://www.readbyqxmd.com/read/29773387/levan-based-fibrous-scaffolds-electrospun-via-co-axial-and-single-needle-techniques-for-tissue-engineering-applications
#6
Gülben Avsar, Deniz Agirbasli, Mehmet Ali Agirbasli, Oguzhan Gunduz, Ebru Toksoy Oner
This represents the first systematic study where levan polysaccharide was used to fabricate fibrous matrices by co-axial and single-needle electrospinning techniques. For this, hydrolyzed (hHL) and sulfated hydrolyzed (ShHL) Halomonas levan were chemically synthesized and used together with polycaprolactone (PCL) and polyethyleneoxide (PEO) for the spinning process. In co-axially spun matrices, ultimate tensile strength (UTS) were found to increase with increasing ShHL concentration and elongation at break of PCL + ShHL matrices increased up to ten-fold when compared to PCL matrices...
August 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29772532/differential-effects-of-the-pi3k-akt-pathway-on-antler-stem-cells-for-generation-and-regeneration-of-antlers-in-vitro
#7
Zhen Liu, Haiping Zhao, Datao Wang, Chris McMahon, Chunyi Li
Understanding the role of the PI3K/AKT pathway in regulating basic antler stem cell parameters and angiogenesis may provide an insight into the mechanisms underlying mammalian appendage development. The present study took multiple approaches in vitro to investigate the effects of the PI3K/AKT pathway on antler stem cells. By addition of LY294002, proliferation rate of the antlerogenic periosteum (AP) cells was decreased significantly ( p <0.01), while the proliferation rate of the pedicle periosteum (PP) cells decreased to a lesser extent; the cytoskeleton of the AP cells was essentially collapsed; and the PP cells significantly shrunken...
June 1, 2018: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/29772481/vascular-peroxide-1-promotes-ox-ldl-induced-programmed-necrosis-in-endothelial-cells-through-a-mechanism-involving-%C3%AE-catenin-signaling
#8
Yin-Zhuang Zhang, Lei Wang, Jie-Jie Zhang, Xiao-Ming Xiong, Di Zhang, Xuan-Meng Tang, Xiu-Ju Luo, Qi-Lin Ma, Jun Peng
BACKGROUND AND AIMS: Vascular peroxidase 1 (VPO1) plays a key role in mediation of cardiovascular oxidative injury. This study aims to determine whether VPO1 can promote programmed necrosis of endothelial cells and the underlying mechanisms. METHODS AND RESULTS: Human umbilical vein endothelial cells (HUVECs) were incubated with oxidized low-density lipoprotein (ox-LDL, 100 μg/mL) for 48 h to induce cell injury, which showed an elevation in cell necrosis (reflected by the increased propidium iodide (PI) positive-staining cells, LDH release and decreased cell viability), concomitant with an increase in programmed necrosis-relevant proteins including receptor-interacting protein kinase 1/3 (RIPK1/3), p-RIPK3 and mixed lineage kinase domain like (MLKL); these phenomena were attenuated by necrostatin-1(Nec-1) and RIPK3 siRNA...
May 3, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29772235/autophagy-mediates-enhancement-of-proangiogenic-activity-by-hypoxia-in-mesenchymal-stromal-stem-cells
#9
Seul-Gi Lee, Young Ae Joe
Mesenchymal stromal/stem cells (MSCs) have been promising source for regenerative cell therapy in ischemic diseases. To improve efficacy of MSC therapy, various priming methods have been developed, and hypoxic priming has been reported to enhance therapeutic efficacy of MSCs by increasing secretion level of growth factors and cytokines. Recently, it has been reported that bone marrow MSCs primed with hypoxic condition show an increase of autophagy. Here, we addressed whether proangiogenic activity increased by hypoxic condition is associated with autophagy...
May 14, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29771821/the-effects-of-exercise-aspirin-and-celecoxib-in-an-atherogenic-environment
#10
Heather Grimm, Jan Kretzschmar, Marc D Cook, Michael D Brown
INTRODUCTION: Optimal vascular function is a hallmark of cardiovascular health. Specifically, the balance of vasoconstricting and vasodilating substances is recognized as a marker of vascular health. One of the greatest challenges to vascular health and vasodilatory balance is tumor necrosis factor alpha (TNFα) mediated inflammation. Uncovering effective strategies that maintain a vascular environment that is more vasodilatory and anti-thrombotic in the face of an inflammatory challenge is favorable...
April 30, 2018: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/29770592/particles-of-vaterite-a-metastable-caco-3-polymorph-exhibit-high-biocompatibility-for-human-osteoblasts-and-endothelial-cells-and-may-serve-as-a-biomaterial-for-rapid-bone-regeneration
#11
Romina Schröder, Laura Besch, Hannah Pohlit, Martin Panthöfer, Wilfried Roth, Holger Frey, Wolfgang Tremel, Ronald E Unger
We have previously described a promising alternative to conventional synthetic bone biomaterials using vaterite, a metastable CaCO3 polymorph, that increases the local Ca2+ concentration in vitro and leads to an over-saturation of phosphate, the primary bone mineral. This stimulates a natural bone-like mineralization in a short period of time. In this study, sterile and endotoxin-free vaterite particles were synthesized in a nearly quantitative yield. The 500-1000 nm vaterite particles did not exhibit any cytotoxic effects as measured by MTT, LDH or crystal violet assays on the human osteoblast cell line (MG-63) exposed to concentrations up to 500 μg/ml vaterite up to 72 hr...
May 16, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29770525/establishment-and-characterization-of-an-embryonic-pericyte-cell-line
#12
Huaning Zhao, Jordan Darden, John C Chappell
OBJECTIVE: Pericytes are specialized perivascular cells embedded within the basement membrane. These cells envelope the abluminal surface of endothelial cells and promote microvessel homeostasis. Recent discoveries of unique pericyte functions, particularly in neural tissues, underscore the need for overcoming existing challenges in establishing a functionally validated pericyte cell line. Here, we present methodologies for addressing these challenges as well as an embryonic pericyte cell line for use with in vitro and ex vivo experimental models...
May 16, 2018: Microcirculation: the Official Journal of the Microcirculatory Society, Inc
https://www.readbyqxmd.com/read/29766570/melatonin-improves-endothelial-function-in-vitro-and-prolongs-pregnancy-in-women-with-early-onset-preeclampsia
#13
Sebastian R Hobson, Seshi Gurusinghe, Rebecca Lim, Nicole O Alers, Suzanne L Miller, John C Kingdom, Euan M Wallace
Preeclampsia remains a leading cause of maternal and perinatal morbidity and mortality. There have been no material advances in the treatment of preeclampsia for nearly 50 years. Combining in vitro studies and a clinical trial, we aimed to determine if melatonin could be a useful adjuvant therapy. In a xanthine/xanthine oxidase (X/XO) placental explant model, melatonin reduced oxidative stress (8-isoprostane) and enhanced antioxidant markers (Nrf2 translocation, HO-1), but did not affect explant production of anti-angiogenic factors (sFlt, sEng, activin A)...
May 16, 2018: Journal of Pineal Research
https://www.readbyqxmd.com/read/29765073/oncotically-driven-control-over-glycocalyx-dimension-for-cell-surface-engineering-and-protein-binding-in-the-longitudinal-direction
#14
Erika M J Siren, Rafi Chapanian, Iren Constantinescu, Donald E Brooks, Jayachandran N Kizhakkedathu
Here we present a simple technique for re-directing reactions on the cell surface to the outermost region of the glycocalyx. Macromolecular crowding with inert polymers was utilized to reversibly alter the accessibility of glycocalyx proteoglycans toward cell-surface reactive probes allowing for reactivity control in the longitudinal direction ('z'-direction) on the glycocalyx. Studies in HUVECs demonstrated an oncotically driven collapse of the glycocalyx brush structure in the presence of crowders as the mechanism responsible for re-directing reactivity...
May 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29763918/comprehensive-effects-of-suppression-of-microrna-383-in-human-bone-marrow-derived-mesenchymal-stem-cells-on-treating-spinal-cord-injury
#15
Guo-Jun Wei, Ke-Wen Zheng, Gang An, Zuo-Wei Shi, Kai-Fu Wang, Ying Guan, Yan-Song Wang, Peng-Fei Li, Da-Ming Dong
BACKGROUND/AIMS: Transplantation of bone-marrow-derived mesenchymal stem cells (MSCs) promotes neural cell regeneration after spinal cord injury (SCI). Recently, we showed that suppression of microRNA-383 (miR-383) in MSCs increased the protein levels of glial cell line derived neurotrophic factor (GDNF), resulting in improved therapeutic effects on SCI. However, the overall effects of miR-383 suppression in MSCs on SCI therapy were not determined yet. Here, we addressed this question...
May 10, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29762844/free-fatty-acids-mediates-human-umbilical-vein-endothelial-cells-inflammation-through-toll-like-receptor-4
#16
L Chen, C-X Yu, B Song, W Cai, C Liu, Q-B Guan
OBJECTIVE: To investigate the role of Toll-like receptor-4 (TLR4) in the free fatty acids (FFAs) induced human umbilical vein endothelial cells (HUVECs) inflammation and to explore the underlying mechanisms. MATERIALS AND METHODS: HUVECs and HEK293 cell lines were obtained from Shanghai Type Culture Collection. Cell counting kit-8 (CCK8) and flow cytometry (FCM) were performed to examine the cell viability and apoptosis rate of HUVECs induced by FFAs treatments with or without infection of toll-like receptor-4 interference (TLR4i) adenovirus...
April 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29762492/zika-virus-induces-autophagy-in-human-umbilical-vein-endothelial-cells
#17
Haoran Peng, Bin Liu, Toure Doueu Yves, Yanhua He, Shijie Wang, Hailin Tang, Hao Ren, Ping Zhao, Zhongtian Qi, Zhaoling Qin
Autophagy is a common strategy for cell protection; however, some viruses can in turn adopt cellular autophagy to promote viral replication. Zika virus (ZIKV) is the pathogen that causes Zika viral disease, and it is a mosquito-borne virus. However, its pathogenesis, especially the interaction between ZIKV and target cells during the early stages of infection, is still unclear. In this study, we demonstrate that infecting human umbilical vein endothelial cells (HUVEC) with ZIKV triggers cellular autophagy. We observed both an increase in the conversion of LC3-I to LC3-II and increased accumulation of fluorescent cells with LC3 dots, which are considered to be the two key indicators of autophagy...
May 15, 2018: Viruses
https://www.readbyqxmd.com/read/29760703/the-increased-endogenous-sulfur-dioxide-acts-as-a-compensatory-mechanism-for-the-downregulated-endogenous-hydrogen-sulfide-pathway-in-the-endothelial-cell-inflammation
#18
Da Zhang, Xiuli Wang, Xiaoyu Tian, Lulu Zhang, Guosheng Yang, Yinghong Tao, Chen Liang, Kun Li, Xiaoqi Yu, Xinjing Tang, Chaoshu Tang, Jing Zhou, Wei Kong, Junbao Du, Yaqian Huang, Hongfang Jin
Endogenous hydrogen sulfide (H2 S) and sulfur dioxide (SO2 ) are regarded as important regulators to control endothelial cell function and protect endothelial cell against various injuries. In our present study, we aimed to investigate the effect of endogenous H2 S on the SO2 generation in the endothelial cells and explore its significance in the endothelial inflammation in vitro and in vivo . The human umbilical vein endothelial cell (HUVEC) line (EA.hy926), primary HUVECs, primary rat pulmonary artery endothelial cells (RPAECs), and purified aspartate aminotransferase (AAT) protein from pig heart were used for in vitro experiments...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29758328/regulation-of-yes-associated-protein-by-laminar-flow
#19
Gautham Chitragari, Sherif Y Shalaby, Brandon J Sumpio, Jiro Kurita, Bauer E Sumpio
BACKGROUND: The cells lining the endothelium of blood vessels are recognized as playing critical roles in vascular health and disease. The mechanisms which regulate endothelial cells (EC) proliferation and release of mediators remains poorly understood but represent a potential source of disease modulation. Actin-cytoskeleton remodeling and cell shape have been suggested as key regulators of phosphorylation of yes associated protein(YAP) which controls cellular growth and proliferation...
May 11, 2018: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/29755642/an-improved-method-for-increasing-the-efficiency-of-gene-transfection-and-transduction
#20
Baomin Shi, Mengzhou Xue, Yi Wang, Yufeng Wang, Davey Li, Xiaomin Zhao, Xinbo Li
Transfection and transduction using lentivirus has gained attention in biomedical research. To date, how to reach the maximum transfection and viral transduction efficiency is still challenging. Here we compared the transfection and viral transduction efficiency using commercially available transfection reagents including FuGENE 6, Lipofectamine 2000 and Lipofectamine 3000 in different cell lines and primary cultured cells. Enhanced green fluorescent protein (EGFP) was clearly seen in Eppendorf tubes from harvested cells using Lipofectamine 3000 without using a microscope and UV activation...
2018: International Journal of Physiology, Pathophysiology and Pharmacology
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