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https://www.readbyqxmd.com/read/28531227/different-microrna-profiles-reveal-the-diverse-outcomes-induced-by-ev71-and-ca16-infection-in-human-umbilical-vein-endothelial-cells-using-high-throughput-sequencing
#1
Jie Song, Yajie Hu, Jiaqi Li, Huiwen Zheng, Jingjing Wang, Lei Guo, Ruotong Ning, Hongzhe Li, Zening Yang, Haitao Fan, Longding Liu
Enterovirus 71 (EV71) and Coxsackievirus A16 (CA16) remain the predominant pathogens in hand, foot, and mouth disease (HFMD), but the factors underlying the pathogenesis of EV71 and CA16 infections have not been elucidated. Recently, the functions of microRNAs (miRNAs) in pathogen-host interactions have been highlighted. In the present study, we performed comprehensive miRNA profiling in EV71- and CA16-infected human umbilical vein endothelial cells (HUVECs) at multiple time points using high-throughput sequencing...
2017: PloS One
https://www.readbyqxmd.com/read/28529061/benzo-a-pyrene-7-8-diol-9-10-epoxide-suppresses-the-migration-and-invasion-of-human-extravillous-trophoblast-htr-8-svneo-cells-by-down-regulating-mmp2-through-inhibition-of-fak-src-pi3k-akt-pathway
#2
Rong Wang, Weiping Wang, Lin Ao, Zhi Wang, Xianglin Hao, Huidong Zhang
Moderate invasion of trophoblasts into the endometrium is crucial for successful pregnancy. Benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) is a carcinogenic metabolite of benzo[a]pyrene which causes various diseases. We investigated the effects of BPDE on migration and invasion of trophoblast HTR-8/SVneo cells. Migration and invasion of cells exposed to 0.25-1.0μM BPDE for 24h were significantly inhibited. Moreover, tube formation of human umbilical vein endothelial cell (HUVEC) was also significantly reduced after incubation with HTR-8/SVneo cells treated with 0...
May 18, 2017: Toxicology
https://www.readbyqxmd.com/read/28527816/adar1-is-targeted-by-mir-143-to-regulate-il-1%C3%AE-induced-endothelial-activation-through-the-nf%C3%AE%C2%BAb-pathway
#3
Yuanzhuo Chen, Hu Peng, Shuqin Zhou, Yugang Zhuang
Excessive endothelial activation by inflammatory mediators is a critical contributing factor of sepsis pathophysiology. ADAR1 (adenosine deaminase acting on RNA), an enzyme that binds and edits double-stranded RNAs, exhibits immune regulatory properties. Whether ADAR1 is involved in the pathophysiology of sepsis is unclear. In the present study, we used human umbilical endothelial cells (HUVECs) as an in vitro model system to investigate the roles of ADAR1 in interleukin (IL)-1β-induced endothelial activation...
May 17, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28525888/combination-of-desi2-and-ip10-gene-therapy-significantly-improves-therapeutic-efficacy-against-murine-carcinoma
#4
Chao Lin, HuaYing Yan, Jun Yang, Lei Li, Mei Tang, Xinyu Zhao, Chunlai Nie, Na Luo, Yuquan Wei, Zhu Yuan
DESI2 (also known as PNAS-4) is a novel pro-apoptotic gene activated during the early response to DNA damage. We previously reported that overexpression of DESI2 induces S phase arrest and apoptosis by activating checkpoint kinases. The present study was designed to test whether combination of DESI2 and IP10 could improve the therapy efficacy in vitro and in vivo. The recombinant plasmid co-expressing DESI2 and IP10 was encapsulated with DOTAP/Cholesterol nanoparticle. Immunocompetent mice bearing CT26 colon carcinoma and LL2 lung cancer were treated with the complex...
May 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28525819/combination-of-flow-and-micropattern-alignment-affecting-flow-resistant-endothelial-cell-adhesion
#5
Xianghui Gong, Jie Yao, Hongping He, Xixi Zhao, Xiaoyi Liu, Feng Zhao, Yan Sun, Yubo Fan
Assuring cell adhesion to an underlying biomaterial surface under blood flow is vital to functional vascular grafts design. In vivo endothelial cells (ECs) are located under the microenvironment of both surface topography of the basement membrane and the mechanical loading resulting from blood flow. Both topographical and mechanical factors should thus be considered when designing vascular grafts to enhance the flow-resistant EC adhesion. This study aims to investigate effects of integrating biomaterial surface topography and flow on EC adhesion, which was a deficit in previous studies...
May 2, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28524367/transplantation-of-human-villous-trophoblasts-preserves-cardiac-function-in-mice-with-acute-myocardial-infarction
#6
Zegen Wang, Ningzheng Dong, Yayan Niu, Zhiwei Zhang, Ce Zhang, Meng Liu, Tiantian Zhou, Qingyu Wu, Ke Cheng
Over the past decade, cell therapies have provided promising strategies for the treatment of ischaemic cardiomyopathy. Particularly, the beneficial effects of stem cells, including bone marrow stem cells (BMSCs), endothelial progenitor cells (EPCs), mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), and induced pluripotent stem cells (iPSCs), have been demonstrated by substantial preclinical and clinical studies. Nevertheless stem cell therapy is not always safe and effective. Hence, there is an urgent need for alternative sources of cells to promote cardiac regeneration...
May 19, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28522006/the-influence-of-solvent-formulations-on-thermosensitive-hydroxybutyl-chitosan-hydrogel-as-a-potential-delivery-matrix-for-cell-therapy
#7
Zixian Bao, Changqing Jiang, Zhiguo Wang, Qiuxia Ji, Guohui Sun, Shichao Bi, Ya Liu, Xiguang Chen
Many cell delivery matrices have been developed due to the low transplantation efficiency of cell therapy. In the present study, thermosensitive hydroxybutyl chitosan (HBC) hydrogels were prepared with different formulations' solvent (Dulbecco's modified eagle's medium/phosphate buffered saline [DMEM/PBS], 0:100, 30:70, 50:50, 70:30, 100:0 [v/v]). Their gelation temperature was raised with DMEM ratio increase (from 9.5°C to 20.9°C). Pore sizes of HBC hydrogels treated with high ionic strength solutions became smaller...
August 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28518223/low-shear-stress-induces-endothelial-reactive-oxygen-species-via-the-at1r-enos-no-pathway
#8
Yuelin Chao, Peng Ye, Linlin Zhu, Xiangquan Kong, Xinliang Qu, Junxia Zhang, Jie Luo, Hongfeng Yang, Shaoliang Chen
Reactive oxygen species (ROS) contribute to many aspects of physiological and pathological cardiovascular processes. However, the underlying mechanism of ROS induction by low shear stress (LSS) remains unclear. Accumulating evidence has shown that the angiotensin II type 1 receptor (AT1R) is involved in inflammation, apoptosis, and ROS production. Our aim was to explore the role of AT1R in LSS-mediated ROS induction. We exposed human umbilical vein endothelial cells (HUVECs) to LSS (3 dyne/cm(2) ) for different periods of time...
May 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28518114/preparation-and-in-vitro-characterization-of-magnetized-mir-modified-endothelial-cells
#9
Natalia Voronina, Heiko Lemcke, Frank Wiekhorst, Jens-Peter Kühn, Markus Frank, Gustav Steinhoff, Robert David
To date, the available surgical and pharmacological treatments for cardiovascular diseases (CVD) are limited and often palliative. At the same time, gene and cell therapies are highly promising alternative approaches for CVD treatment. However, the broad clinical application of gene therapy is greatly limited by the lack of suitable gene delivery systems. The development of appropriate gene delivery vectors can provide a solution to current challenges in cell therapy. In particular, existing drawbacks, such as limited efficiency and low cell retention in the injured organ, could be overcome by appropriate cell engineering (i...
May 2, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28514506/human-adipose-derived-mesenchymal-stem-cell-secreted-cxcl1-and-cxcl8-facilitate-breast-tumor-growth-by-promoting-angiogenesis
#10
Yuan Wang, Junli Liu, Qingyuan Jiang, Jie Deng, Fen Xu, Xiaolei Chen, Fuyi Cheng, Yujing Zhang, Yunqi Yao, Zhemin Xia, Xia Xu, Xiaolan Su, Meijuan Huang, Lei Dai, Yang Yang, Shuang Zhang, Dechao Yu, Robert Chunhua Zhao, Yuquan Wei, Hongxin Deng
Autologous adipose tissue or adipose tissue with additive adipose-derived mesenchymal stem cells (ADSCs) is used in the breast reconstruction of breast cancer patients who undergo mastectomy. ADSCs play an important role in the angiogenesis and adipogenesis, which make it much better than other materials. However, ADSCs may promote residual tumor cells to proliferate or metastasize, and the mechanism is still not fully understood. In our present study, we demonstrated that hADSCs could facilitate tumor cells growth after co-injection with MCF7 and ZR-75-30 breast cancer cells (BCCs) by promoting angiogenesis, but hADSCs showed limited effect on the growth of MDA-MB-231 BCCs...
May 17, 2017: Stem Cells
https://www.readbyqxmd.com/read/28514291/role-of-microrna-130a-in-the-pathogeneses-of-obstructive-sleep-apnea-hypopnea-syndrome-associated-pulmonary-hypertension-by-targeting-the-gax-gene
#11
Zhe An, Dan Wang, Guang Yang, Wen-Qi Zhang, Jin Ren, Jin-Ling Fu
OBJECTIVE: The purpose of this study was to elucidate the role of microRNA-130a (miR-130a) in obstructive sleep apnea hypopnea syndrome (OSAHS)-associated pulmonary hypertension (PHT) by targeting the growth arrest-specific homeobox (GAX) gene. METHODS: A total of 108 patients with OSAHS-associated PHT were recruited as the OSAHS-associated PHT group and 110 healthy individuals were randomly selected as the normal control group. Human umbilical vein endothelial cells (HUVECs) were selected and divided into the control, miR-130a mimic, mimic negative control (NC), miR-130a inhibitor, and inhibitor-NC groups...
May 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28513223/activation-of-thromboxane-a2-receptors-mediates-endothelial-dysfunction-in-diabetic-mice
#12
Xiaona Xie, Wanchun Sun, Jun Wang, Xiaoou Li, Xiaofeng Liu, Ning Liu
BACKGROUND: Diabetes is one of high-risk factors for cardiovascular disease. Improvement of endothelial dysfunction in diabetes reduces vascular complications. However, the underlying mechanism needs to be uncovered. This study was conducted to elucidate whether and how thromboxane A2 receptor (TPr) activation contributes to endothelial dysfunction in diabetes. METHODS AND RESULTS: Exposure of human umbilical vein endothelial cells (HUVECs) to either TPr agonists, two structurally related thromboxane A2 (TxA2) mimetics, significantly reduced phosphorylations of endothelial nitric oxide synthase (eNOS) at Ser(1177) and Akt at Ser(473)...
May 17, 2017: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/28512525/h2s-donor-nahs-changes-the-production-of-endogenous-h2s-and-no-in-d-galactose-induced-accelerated-ageing
#13
Wei Wu, Cui-Lan Hou, Xue-Pan Mu, Chen Sun, Yi-Chun Zhu, Ming-Jie Wang, Qian-Zhou Lv
Aims. The study was designed to explore whether hydrogen sulphide (H2S) and nitric oxide (NO) generation changed in D-galactose- (D-gal-) induced ageing, the possible effects of exogenous H2S supplementation, and related mechanisms. Results. In D-gal-induced senescent mice, both H2S and NO levels in the heart, liver, and kidney tissues were decreased significantly. A similar trend was observed in D-gal-challenged human umbilical vein endothelial cells (HUVECs). Sustained H2S donor (NaHS) treatment for 2 months elevated H2S and NO levels in these mice, and during this period, the D-gal-induced senescent phenotype was reversed...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28512254/catecholamines-facilitate-vegf-dependent-angiogenesis-via-%C3%AE-2-adrenoceptor-induced-epac1-and-pka-activation
#14
Jaspal Garg, Yu-Xi Feng, Sepp R Jansen, Julian Friedrich, Frank Lezoualc'h, Martina Schmidt, Thomas Wieland
Chronic stress has been associated with the progression of cancer and antagonists for β-adrenoceptors (βAR) are regarded as therapeutic option. As they are also used to treat hemangiomas as well as retinopathy of prematurity, a role of endothelial β2AR in angiogenesis can be envisioned. We therefore investigated the role of β2AR-induced cAMP formation by analyzing the role of the cAMP effector molecules exchange factor directly activated by cAMP 1 (Epac1) and protein kinase A (PKA) in endothelial cells (EC)...
April 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/28512172/in-vivo-hemin-conditioning-targets-the-vascular-and-immunological-compartments-and-restrains-prostate-tumor-development
#15
Felipe M Jaworski, Lucas Gentilini, Geraldine Gueron, Roberto P Meiss, Emiliano G Ortiz, Paula M Berguer, Asif Ahmed, Nora M Navone, Gabriel A Rabinovich, Daniel Compagno, Diego J Laderach, Elba S Vazquez
Purpose: Conditioning strategies constitute a relatively unexplored and exciting opportunity to shape tumor fate by targeting the tumor microenvironment. In this study we assessed how hemin, a pharmacological inducer of Heme Oxygenase-1 (HO-1), impacts upon prostate cancer (PCa) development in an in vivo conditioning model. <p>Experimental Design: The stroma of C57BL/6 mice was conditioned by subcutaneous administration of hemin prior to TRAMP-C1 tumor challenge. Complementary in vitro and in vivo assays were performed to evaluate hemin effect on both angiogenesis and the immune response...
May 16, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28511963/sirna-targeting-net1-reduces-the-angiogenesis-and-tumor-growth-of-in-vivo-cervical-squamous-cell-carcinoma-through-vegf-down-regulation
#16
Yuting Zhang, Pei Xia, Wenhui Zhang, Min Yan, Xiujuan Xiong, Weiwei Yu, Enlin Song
Net1, a guanine nucleotide exchange factor, is implicated in cancer cell invasion through activation of RhoA. However, there is still no report on the association between Net1 and cancer angiogenesis. The current study was designed to explore the roles of Net1 in the angiogenesis of cervical squamous cell carcinoma (CSCC) and further observe the effects of Net1 siRNA on the tumor growth. Net1 was overexpressed in CSCC samples (n=80), correlated to the cancer microvessel density (MVD, r=0.223, P=.026) and related to aggressive clinical behaviors, including depth of cervical wall invasion (P=...
May 13, 2017: Human Pathology
https://www.readbyqxmd.com/read/28511903/novel-role-of-the-nutraceutical-bioactive-compound-berberine-in-lectin-like-oxldl-receptor-1-mediated-endothelial-dysfunction-in-comparison-to-lovastatin
#17
C Caliceti, P Rizzo, R Ferrari, F Fortini, G Aquila, E Leoncini, L Zambonin, B Rizzo, D Calabria, P Simoni, M Mirasoli, M Guardigli, S Hrelia, A Roda, A F G Cicero
BACKGROUND AND AIMS: Oxidized LDL (oxLDL) or pro-inflammatory stimuli lead to increased oxidative stress linked to endothelial dysfunction and atherosclerosis. The oxLDL receptor-1 (LOX1) is elevated within atheromas and cholesterol-lowering statins inhibit LOX1 expression. Berberine (BBR), an alkaloid extracted from plants of gender Berberis, has lipid-lowering and anti-inflammatory activity. However, its role in regulating LOX1-mediated signaling is still unknown. The aim of this study was to investigate the effect of BBR on oxLDL- and TNFα-induced endothelial dysfunction in human umbilical vein endothelial cells (HUVECs) and to compare it with that of lovastatin (LOVA)...
April 19, 2017: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
https://www.readbyqxmd.com/read/28511014/protein-corona-formation-on-colloidal-polymeric-nanoparticles-and-polymeric-nanogels-impact-on-cellular-uptake-toxicity-immunogenicity-and-drug-release-properties
#18
Katja Obst, Guy Yealland, Benjamin Balzus, Enrico Miceli, Mathias Dimde, Christoph Weise, Murat Eravci, Roland Bodmeier, Rainer Haag, Marcelo Calderon, Nada Charbaji, Sarah Hedtrich
The adsorption of biomolecules to the surfaces of a nanoparticle (NP) following administration into biological environments is widely recognised. In particular, the "protein corona" is well understood in terms of formation kinetics and its impact upon the biological interactions of NPs. Its presence is an essential consideration in the design of therapeutic NPs. In the present study, the protein corona of six polymeric nanoparticles of prospective therapeutic use, were investigated. These included three colloidal NPs-soft core-multi-shell (CMS) NPs, plus solid cationic Eudragit® RS (EGRS) and anionic ethyl cellulose (EC) nanoparticles-and three nanogels (NGs)-thermoresponsive dendritic-polyglycerol (dPG) nanogels (NGs) and two amino-functionalised dPG-NGs...
May 16, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28510269/kallikrein-related-peptidase-4-induces-cancer-associated-fibroblast-features-in-prostate-derived-stromal-cells
#19
T Kryza, L M Silva, N Bock, R A Fuhrman-Luck, C R Stephens, J Gao, H Samaratunga, M G Lawrence, J D Hooper, Y Dong, G P Risbridger, J A Clements
The reciprocal communication between cancer cells and their microenvironment is critical in cancer progression. Although involvement of cancer-associated fibroblasts (CAF) in cancer progression is long established, the molecular mechanisms leading to differentiation of CAFs from normal fibroblasts are poorly understood. Here, we report that kallikrein-related peptidase-4 (KLK4) promotes CAF differentiation. KLK4 is highly expressed in prostate epithelial cells of premalignant (prostatic intra-epithelial neoplasia) and malignant lesions compared to normal prostate epithelia, especially at the peri-stromal interface...
May 16, 2017: Molecular Oncology
https://www.readbyqxmd.com/read/28510249/mechanism-of-pingyangmycin-induced-apoptosis-of-cultured-human-umbilical-vein-endothelial-cells
#20
Y D Huang, X Tong, Q Chen, S W Xia, Y Zhuo, Z H Kang, X Y Xue
In this study, we investigated the effects of pingyangmycin (PYM) on the growth inhibition and apoptosis of human umbilical vein endothelial cells (HUVEC). In this study, we aimed to explore the optimal concentration of PYM to induce the apoptosis of HUVEC and to determine its mechanism of action. After treatment of HUVEC with different concentrations of PYM for 24 h, cell counting kit-8 (CCK-8) was used to detect growth inhibiting effects. Annexin V-FITC/propidium iodide stain was used to detect apoptosis, and western blot was used to detect the expression of glucose-related protein 78 (GPR78) and C/EBP homologous protein (CHOP) endoplasmic reticulum stress proteins...
May 10, 2017: Genetics and Molecular Research: GMR
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