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https://www.readbyqxmd.com/read/28722147/surface-isoelectric-focusing-sief-with-carrier-ampholyte-ph-gradient
#1
Zhichao Wang, Cornelius Ivory, Adrienne R Minerick
Isoelectric focusing (IEF) is a powerful tool for amphoteric protein separations because of high sensitivity, bio-compatibility, and reduced complexity compared to chromatography or mechanical separation techniques. IEF miniaturization is attractive because it enables rapid analysis, easier adaptation to point of care applications, and smaller sample demands. However, existing small-scale IEF tools have not yet been able to analyze single protein spots from array libraries, which are ubiquitous in many pharmaceutical discovery and screening protocols...
July 19, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28722137/precise-and-arbitrary-deposition-of-biomolecules-onto-biomimetic-fibrous-matrices-for-spatially-controlled-cell-distribution-and-functions
#2
Chao Jia, Bowen Luo, Haoyu Wang, Yongqian Bian, Xueyong Li, Shaohua Li, Hongjun Wang
Advances in nano-/microfabrication allow the fabrication of biomimetic substrates for various biomedical applications. In particular, it would be beneficial to control the distribution of cells and relevant biomolecules on an extracellular matrix (ECM)-like substrate with arbitrary micropatterns. In this regard, the possibilities of patterning biomolecules and cells on nanofibrous matrices are explored here by combining inkjet printing and electrospinning. Upon investigation of key parameters for patterning accuracy and reproducibility, three independent studies are performed to demonstrate the potential of this platform for: i) transforming growth factor (TGF)-β1-induced spatial differentiation of fibroblasts, ii) spatiotemporal interactions between breast cancer cells and stromal cells, and iii) cancer-regulated angiogenesis...
July 19, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28720761/parylene-c-topographic-micropattern-as-a-template-for-patterning-pdms-and-polyacrylamide-hydrogel
#3
Ilaria Sanzari, Mauro Callisti, Antonio De Grazia, Daniel J Evans, Tomas Polcar, Themistoklis Prodromakis
Parylene C is a well-known polymer and it has been mainly employed as a protective layer for implantable electronics. In this paper, we propose a new approach to use Parylene C as a versatile template for patterning soft materials potentially applicable as scaffolds in cardiac tissue engineering (TE). Parylene C substrates were anisotropically patterned through standard lithographic process with hydrophilic channels separating raised hydrophobic strips. Ridges and grooves of the template are 10 µm width and depth ranging from 1 to 17 µm...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28707348/regulation-of-cell-arrangement-using-a-novel-composite-micropattern
#4
Xiaoyi Liu, Yaoping Liu, Feng Zhao, Tingting Hun, Shan Li, Yuguang Wang, Weijie Sun, Wei Wang, Yan Sun, Yubo Fan
Micropatterning technique has been used to control single cell geometry in many researches, however, this is no report that it is used to control multicelluar geometry, which not only control single cell geometry but also organize those cells by a certain pattern. In this work, a composite protein micropattern is developed to control both cell shape and cell location simultaneously. The composite micropattern consists of a central circle 15 μm in diameter for single-cell capture, surrounded by small, square arrays (3 μm × 3 μm) for cell spreading...
July 13, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28702667/microengineered-cultures-containing-human-hepatic-stellate-cells-and-hepatocytes-for-drug-development
#5
Matthew D Davidson, David A Kukla, Salman R Khetani
In non-alcoholic steatohepatitis (NASH), hepatic stellate cells (HSC) differentiate into myofibroblast-like cells that cause fibrosis, which predisposes patients to cirrhosis and hepatocellular carcinoma. Thus, modeling interactions between activated HSCs and hepatocytes in vitro can aid in the development of anti-NASH/fibrosis therapeutics and lead to a better understanding of disease progression. Species-specific differences in drug metabolism and disease pathways now necessitate the supplementation of animal studies with data acquired using human liver models; however, current models do not adequately model the negative effects of primary human activated HSCs on the phenotype of otherwise well-differentiated primary human hepatocytes (PHHs) as in vivo...
July 12, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/28671816/photopolymerizable-zwitterionic-polymer-patterns-control-cell-adhesion-and-guide-neural-growth
#6
Braden L Leigh, Elise Cheng, Linjing Xu, Corinne Andresen, Marlan R Hansen, C Allan Guymon
Developing materials that reduce or eliminate fibrosis encapsulation of neural prosthetic implants could significantly enhance implant fidelity by improving the tissue/electrode array interface. Here, we report on the photografting and patterning of two zwitterionic materials, sulfobetaine methacrylate (SBMA) and carboxybetaine methacrylate (CBMA) for controlling the adhesion and directionality of cells relevant to neural prosthetics. CBMA and SBMA polymers were photopolymerized and grafted on glass surfaces then characterized by X-ray photoelectron spectroscopy, water contact angle, and protein adsorption...
July 3, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28671108/cell-instructive-high-resolution-micropatterned-polylactic-acid-surfaces
#7
David Baiao Barata, Paulo Dias, Paul Wieringa, Clemens A van Blitterswijk, Pamela Habibovic
Micro- and nanoscale topographical structuring of biomaterial surfaces has been a valuable tool for influencing cell behavior, including cell attachment, proliferation and differentiation. However, most fabrication techniques for surface patterning of implantable biomaterials suffer from a limited resolution, not allowing controlled generation of sub-cellular three-dimensional features. Here, a direct laser lithography technique based on two-photon absorption was used to construct several patterns varying in size between 500 nm and 15 µm...
July 3, 2017: Biofabrication
https://www.readbyqxmd.com/read/28657075/bioinspired-onion-epithelium-like-structure-promotes-the-maturation-of-cardiomyocytes-derived-from-human-pluripotent-stem-cells
#8
Cong Xu, Li Wang, Yue Yu, Fangchao Yin, Xiaoqing Zhang, Lei Jiang, Jianhua Qin
Organized cardiomyocyte alignment is critical to maintain the mechanical properties of the heart. In this study, we present a new and simple strategy to fabricate a biomimetic microchip designed with an onion epithelium-like structure and investigate the guided behavior of human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) on the substrate. The hiPSC-CMs were observed to be confined by the three dimensional surficial features morphologically, analogous to the in vivo microenvironment, and exhibited an organized anisotropic alignment on the onion epithelium-like structure with good beating function...
June 28, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28656905/probing-cooperative-force-generation-in-collective-cancer-invasion
#9
Amani A Alobaidi, Yaopengxiao Xu, Shaohua Chen, Yang Jiao, Bo Sun
Collective cellular dynamics in the three-dimensional extracellular matrix (ECM) plays a crucial role in many physiological processes such as cancer invasion. Both chemical and mechanical signaling support cell-cell communications on a variety of length scales, leading to collective migratory behaviors. Here we conduct experiments using 3D in vitro tumor models and develop a phenomenological model in order to probe the cooperativity of force generation in the collective invasion of breast cancer cells. In our model, cell-cell communication is characterized by a single parameter that quantifies the correlation length of cellular migration cycles...
June 28, 2017: Physical Biology
https://www.readbyqxmd.com/read/28643414/associating-and-dissociating-nanodimer-analysis-for-quantifying-ultra-small-amounts-of-dna
#10
Keunsuk Kim, Jeong-Wook Oh, Young Kwang Lee, Jiwoong Son, Jwa-Min Nam
Amplification- and enzyme-free quantification of DNA at ultra-low concentrations, on the orders of 10-1,000 targets, is highly beneficial, but is extremely challenging. To address this challenge, true detection signals should be reliably discriminated from false or noise signals. Here, we developed the dynamically associating and dissociating nanodimer analysis (ADNA) method that allows for collecting maximum detection signals from true target-binding events while keeping nonspecific signals at minimum level...
June 23, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28639984/impact-of-implant-surface-micropatterns-on-epithelial-cell-behavior
#11
Yiping Tong, Zhengyu Yang, Ruoxi Du, Siqian Wang, Qiaozhen Zhou, Chongyun Bao, Jianfeng Ma
PURPOSE: This study investigated the effect of topography on cell behavior by screening polydimethylsiloxane (PDMS) molds with different nanoscale micropatterns to determine the ideal surface characteristics for attachment of human epithelial cells. MATERIALS AND METHODS: A soft PDMS mold with regular dot arrays was fabricated based on an aluminum oxide template with ordered nanotube arrays and used as a substrate for cell culture. Cell proliferation, spread, and morphology, as well as features of the extracellular matrix and the actin cytoskeleton were assessed...
June 19, 2017: Implant Dentistry
https://www.readbyqxmd.com/read/28634948/3d-cell-culture-in-micropatterned-hydrogels-prepared-by-photomask-microneedle-or-soft-lithography-techniques
#12
Seyedsina Moeinzadeh, Esmaiel Jabbari
Despite the advantages of three-dimensional (3D) hydrogels for cell culture over traditional 2D plates, their clinical application is limited by inability to recapitulate the micro-architecture of complex tissues. Micropatterning can be employed to modify the homogenous micro-architecture of hydrogels. Three techniques for cell encapsulation in 3D micropatterned gels are described. The photomask and micromold techniques are used for cell encapsulation in relatively shallow patterns like disks or short rectangles but due to the presence of PDMS mold, the resolution of micromold technique is potentially higher than the photomask...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28628944/bistable-solid-state-fluorescence-switching-in-photoluminescent-infinite-coordination-polymers
#13
Jincheol Kim, Youngmin You, Seong-Jun Yoon, Jong H Kim, Boseok Kang, Sang Kyu Park, Dong Ryeol Whang, Jangwon Seo, Kilwon Cho, Soo Young Park
Photo-functional infinite coordinated polymers (ICPs) were synthesized that consist of the photochromic dithienylethene (DTE) and a luminescent bridging unit to give enhanced fluorescence in the solid state. We could fabricate well-ordered micropatterns of these ICPs by a soft-lithographic method, which repeatedly showed high contrast on-off fluorescence switching.
June 19, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28628129/high-speed-fixed-target-serial-virus-crystallography
#14
Philip Roedig, Helen M Ginn, Tim Pakendorf, Geoff Sutton, Karl Harlos, Thomas S Walter, Jan Meyer, Pontus Fischer, Ramona Duman, Ismo Vartiainen, Bernd Reime, Martin Warmer, Aaron S Brewster, Iris D Young, Tara Michels-Clark, Nicholas K Sauter, Abhay Kotecha, James Kelly, David J Rowlands, Marcin Sikorsky, Silke Nelson, Daniel S Damiani, Roberto Alonso-Mori, Jingshan Ren, Elizabeth E Fry, Christian David, David I Stuart, Armin Wagner, Alke Meents
We report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with the high-speed Roadrunner goniometer for sample delivery, we were able to determine the crystal structures of the picornavirus bovine enterovirus 2 (BEV2) and the cytoplasmic polyhedrosis virus type 18 polyhedrin, with total data collection times of less than 14 and 10 min, respectively...
June 19, 2017: Nature Methods
https://www.readbyqxmd.com/read/28627756/microcontact-peeling-a-cell-micropatterning-technique-for-circumventing-direct-adsorption-of-proteins-to-hydrophobic-pdms
#15
Sho Yokoyama, Tsubasa S Matsui, Shinji Deguchi
Microcontact printing (μCPr) is one of the most popular techniques used for cell micropatterning. In conventional μCPr, a polydimethylsiloxane (PDMS) stamp with microfeatures is used to adsorb extracellular matrix (ECM) proteins onto the featured surface and transfer them onto particular areas of a cell culture substrate. However, some types of functional proteins other than ECM have been reported to denature upon direct adsorption to hydrophobic PDMS. Here we describe a detailed protocol of an alternative technique--microcontact peeling (μCPe)--that allows for cell micropatterning while circumventing the step of adsorbing proteins to bare PDMS...
June 19, 2017: Current Protocols in Cell Biology
https://www.readbyqxmd.com/read/28627043/fabrication-of-millimeter-long-carbon-tubular-nanostructures-using-the-self-rolling-process-inherent-in-elastic-protein-layers
#16
Hyojin Ko, Leila F Deravi, Sung-Jin Park, Jingon Jang, Takhee Lee, Cheong Kang, Jin Seok Lee, Kevin Kit Parker, Kwanwoo Shin
Millimeter-long conducting fibers can be fabricated from carbon nanomaterials via a simple method involving the release of a prestrained protein layer. This study shows how a self-rolling process initiated by polymerization of a micropatterned layer of fibronectin (FN) results in the production of carbon nanomaterial-based microtubular fibers. The process begins with deposition of carbon nanotube (CNT) or graphene oxide (GO) particles on the FN layer. Before polymerization, particles are discrete and nonconducting, but after polymerization the carbon materials become entangled to form an interconnected conducting network clad by FN...
June 19, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28622706/electrical-stimulation-of-microengineered-skeletal-muscle-tissue-effect-of-stimulus-parameters-on-myotube-contractility-and-maturation
#17
Ramin Banan Sadeghian, Majid Ebrahimi, Sahar Salehi
Skeletal muscle tissues engineered in vitro are aneural, short in number of fibers required to function properly, and degenerate rapidly. Electrical stimulation has been widely used to compensate for such lack of neural activity, yet the relationship between the stimulation parameters and the tissue response is subject of debate. Here we study the effect of overnight electrical stimulation (training) on the contractility and maturity of aligned C2C12 myotubes developed on micropatterned gelatin methacryloyl (GelMA) substrates...
June 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28616967/effects-of-functional-groups-of-materials-on-nonspecific-adhesion-and-chondrogenic-induction-of-mesenchymal-stem-cells-on-free-and-micropatterned-surfaces
#18
Bin Cao, Yuanmeng Peng, Xiangnan Liu, Jiandong Ding
Functional groups of materials are known to affect cell behaviors, yet the corresponding effect on stem cell differentiation is always coupled with that of cell spreading; it is thus unclear whether the chemical groups influence cell differentiation directly or via cell spreading indirectly. Herein we used a unique surface patterning technique to decouple the corresponding effects. Mesenchymal stem cells (MSCs) derived from bone marrow were seeded on surfaces coated with alkanethiols with one of four functional end groups (-CH3, -OH, -COOH, and -NH2) and underwent 9 days of chondrogenic induction...
July 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28616133/naked-eye-detection-of-multiple-tumor-related-mrnas-from-patients-with-photonic-crystal-micropattern-supported-dual-modal-upconversion-bioprobes
#19
Xiaoxia Hu, Yingqian Wang, Haoyang Liu, Jie Wang, Yaning Tan, Fubing Wang, Quan Yuan, Weihong Tan
Development of a portable device for the detection of multiple mRNAs is a significant need in the early diagnosis of cancer. We have designed a biochip-based mRNA detection device by combining a hydrophilic-hydrophobic micropattern with upconversion luminescence (UCL) probes. The device achieves highly sensitive detection, using the naked eye, of multiple mRNAs among patient samples. The high sensitivity is attributed to enrichment of the target concentration and a fluorescence enhancement effect. In addition, since the photonic crystal (PC) dot biochip is functionalized with dual-wavelength excitation UCL probes, two kinds of mRNAs in the heterogeneous biological samples are detected simultaneously, and the corresponding luminescence signals are captured using an unmodified camera phone...
January 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28615317/orientation-and-repositioning-of-chromosomes-correlate-with-cell-geometry-dependent-gene-expression
#20
Yejun Wang, Mallika Nagarajan, Caroline Uhler, G V Shivashankar
Extracellular matrix signals from the microenvironment regulate gene expression patterns and cell behavior. Using a combination of experiments and geometric models, we demonstrate correlations between cell geometry, 3D organization of chromosome territories and gene expression. Fluorescence in situ hybridization experiments showed that micropatterned fibroblasts cultured on anisotropic (AP) versus isotropic (IP) substrates resulted in repositioning of specific chromosomes, which contained genes that were differentially regulated by cell geometries...
June 14, 2017: Molecular Biology of the Cell
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