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https://www.readbyqxmd.com/read/28340410/development-of-fusogenic-glass-surfaces-that-impart-spatiotemporal-control-over-macrophage-fusion-direct-visualization-of-multinucleated-giant-cell-formation
#1
James J Faust, Wayne Christenson, Kyle Doudrick, Robert Ros, Tatiana P Ugarova
Implantation of synthetic material, including vascular grafts, pacemakers, etc. results in the foreign body reaction and the formation of multinucleated giant cells (MGCs) at the exterior surface of the implant. Despite the long-standing premise that fusion of mononucleated macrophages results in the formation of MGCs, to date, no published study has shown fusion in context with living specimens. This is due to the fact that optical-quality glass, which is required for the majority of live imaging techniques, does not promote macrophage fusion...
March 2, 2017: Biomaterials
https://www.readbyqxmd.com/read/28292428/cell-cell-contact-area-affects-notch-signaling-and-notch-dependent-patterning
#2
Oren Shaya, Udi Binshtok, Micha Hersch, Dmitri Rivkin, Sheila Weinreb, Liat Amir-Zilberstein, Bassma Khamaisi, Olya Oppenheim, Ravi A Desai, Richard J Goodyear, Guy P Richardson, Christopher S Chen, David Sprinzak
During development, cells undergo dramatic changes in their morphology. By affecting contact geometry, these morphological changes could influence cellular communication. However, it has remained unclear whether and how signaling depends on contact geometry. This question is particularly relevant for Notch signaling, which coordinates neighboring cell fates through direct cell-cell signaling. Using micropatterning with a receptor trans-endocytosis assay, we show that signaling between pairs of cells correlates with their contact area...
March 13, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28289057/applying-stable-isotope-labeled-amino-acids-in-micropatterned-hepatocyte-co-culture-to-directly-determine-the-degradation-rate-constant-for-cyp3a4
#3
Ryan H Takahashi, Sheerin Shahidi-Latham, Susan Wong, Jae H Chang
The rate of enzyme degradation (kdeg) is an important input parameter for the prediction of clinical drug-drug-interactions (DDI) that result from mechanism-based inactivation or induction of cytochrome P450s. Currently, a large range of reported estimates for CYP3A4 enzyme degradation exists, and consequently, large uncertainty exists in steady-state predictions for DDI. In the current investigations, stable isotope labeled amino acids in culture (SILAC) was applied to a long-lived primary human hepatocyte culture, HepatoPac, to directly monitor the degradation of CYP3A4...
March 13, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/28288348/a-microengineered-collagen-scaffold-for-generating-a-polarized-crypt-villus-architecture-of-human-small-intestinal-epithelium
#4
Yuli Wang, Dulan B Gunasekara, Mark I Reed, Matthew DiSalvo, Scott J Bultman, Christopher E Sims, Scott T Magness, Nancy L Allbritton
The human small intestinal epithelium possesses a distinct crypt-villus architecture and tissue polarity in which proliferative cells reside inside crypts while differentiated cells are localized to the villi. Indirect evidence has shown that the processes of differentiation and migration are driven in part by biochemical gradients of factors that specify the polarity of these cellular compartments; however, direct evidence for gradient-driven patterning of this in vivo architecture has been hampered by limitations of the in vitro systems available...
March 6, 2017: Biomaterials
https://www.readbyqxmd.com/read/28277625/cationic-polymerization-from-photoinitiation-to-photocontrol
#5
Quentin Michaudel, Veronika Kottisch, Brett P Fors
During the last 40 years, researchers investigating photoinitiated cationic polymerizations have delivered tremendous success in both industrial and academic settings. A myriad of photoinitiating systems have been developed, allowing for the polymerization of a broad array of monomers (e.g., epoxides, vinyl ethers, alkenes, cyclic ethers, and lactones) under practical, inexpensive, and environmentally benign conditions. More recently, owing to progress in photoredox catalysis, photocontrolled cationic polymerization has emerged as a means to precisely regulate polymer chain growth...
March 9, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28276500/novel-programmable-shape-memory-polystyrene-film-a-thermally-induced-beam-power-splitter
#6
Peng Li, Yu Han, Wenxin Wang, Yanju Liu, Peng Jin, Jinsong Leng
Micro/nanophotonic structures that are capable of optical wave-front shaping are implemented in optical waveguides and passive optical devices to alter the phase of the light propagating through them. The beam division directions and beam power distribution depend on the design of the micro/nanostructures. The ultimate potential of advanced micro/nanophotonic structures is limited by their structurally rigid, functional singleness and not tunable against external impact. Here, we propose a thermally induced optical beam-power splitter concept based on a shape memory polystyrene film with programmable micropatterns...
March 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28273799/a-review-of-structure-construction-of-silk-fibroin-biomaterials-from-single-structures-to-multi-level-structures
#7
REVIEW
Yu Qi, Hui Wang, Kai Wei, Ya Yang, Ru-Yue Zheng, Ick Soo Kim, Ke-Qin Zhang
The biological performance of artificial biomaterials is closely related to their structure characteristics. Cell adhesion, migration, proliferation, and differentiation are all strongly affected by the different scale structures of biomaterials. Silk fibroin (SF), extracted mainly from silkworms, has become a popular biomaterial due to its excellent biocompatibility, exceptional mechanical properties, tunable degradation, ease of processing, and sufficient supply. As a material with excellent processability, SF can be processed into various forms with different structures, including particulate, fiber, film, and three-dimensional (3D) porous scaffolds...
March 3, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28268300/a-simple-method-for-micropatterning-nanofibrous-hydrogel-film
#8
K Higashi, N Miki
This paper propose a new fabrication process for micropatterning a nanofibrous thin film made of bacterial cellulose(BC). BC is a hydrogel produced by specific bacteria and composed of pure cellulosic nanoflbers exhibiting 3D network structure. Such nanofibrous structure is found to be appropriate for adhesion of anchorage-dependent cells. Furthermore, BC shows high biocompatibility and mechanical toughness. Thus, the microfabrication technique for BC broadens potentials for applications. In this study, we report a new method for micropatterning BC film with feature resolution comparable with photolithography technology...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28262902/p-selectin-and-icam-1-synergy-in-mediating-thp-1-monocyte-adhesion-in-hemodynamic-flow-is-length-dependent
#9
Erin Elizabeth Edwards, Susan Napier Thomas
The tightly orchestrated recruitment of monocytes, whose progeny are critical to the progression and resolution of various physiological and pathophysiological processes, is implicated in the time course, severity, and resolution of pathology. Using a microfluidic-based cell adhesion assay integrating spatiotemporal analyses and micropatterning of adhesive proteins, we interrogated the effects of adhesive molecule presentation length, which varies in vivo with disease and stage, on THP-1 monocyte cell rolling versus firm adhesion mediated by P-selectin and/or ICAM-1 in hemodynamic flow...
March 6, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/28255708/revealing-the-role-of-microscale-architecture-in-immune-synapse-function-through-surface-micropatterning
#10
Joung-Hyun Lee, Lance C Kam
The immune synapse has emerged as a compelling example of structural complexity within cell-cell interfaces. This chapter focuses on the use of microcontact printing to isolate and investigate how spatial organization of signaling molecules drives the function of immune cells. In the process detailed here, multiple rounds of microcontact printing are combined to create patterned surfaces that control the relative spatial localization of CD3 and CD28 signaling in T cells, effectively replacing an antigen presenting cell with an engineered surface...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28247751/osteogenic-lineage-commitment-of-adipose-derived-stem-cells-is-predetermined-by-three-dimensional-cell-accumulation-on-micropatterned-surface
#11
Yuichi Furuhata, Toru Yoshitomi, Yuka Kikuchi, Miho Sakao, Keitaro Yoshimoto
Lineage commitment of stem cells is mainly regulated by their microenvironments, which comprise soluble growth factors, extracellular matrix, mechanical forces, and cell density. Although numerous studies have investigated stem cell response to these factors in two-dimensional (2D) culture, little is known about that in 3D culture. Here, we studied effects of 3D cell accumulation levels on the differentiation behavior of mesenchymal stem cells (MSCs) by using a micropatterned surface. After induction of 3D-cultured MSCs on the surface, their osteogenic differentiation was significantly promoted, while adipogenic differentiation was not...
March 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28245360/patterning-three-dimensional-hydrogel-microenvironments-using-hyperbranched-polyglycerols-for-independent-control-of-mesh-size-and-stiffness
#12
Sara Pedron, Amanda M Pritchard, Gretchen A Vincil, Brenda Andrade, Steven C Zimmerman, Brendan A C Harley
The extracellular matrix is an environment rich with structural, mechanical, and molecular signals that can impact cell biology. Traditional approaches in hydrogel biomaterial design often rely on modifying the concentration of cross-linking groups to adjust mechanical properties. However, this strategy provides limited capacity to control additional important parameters in 3D cell culture such as microstructure and molecular diffusivity. Here we describe the use of multifunctional hyperbranched polyglycerols (HPGs) to manipulate the mechanical properties of polyethylene glycol (PEG) hydrogels while not altering biomolecule diffusion...
March 9, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28244722/micropatterned-pyramidal-ionic-gels-for-sensing-broad-range-pressures-with-high-sensitivity
#13
Sung Hwan Cho, Seung Won Lee, Seunggun Yu, Hyeohn Kim, Sooho Chang, Donyoung Kang, Ihn Hwang, Han Sol Kang, Beomjin Jeong, Eui Hyuk Kim, Suk Man Cho, Kang Lib Kim, Hyungsuk Lee, Wooyoung Shim, Cheolmin Park
The development of pressure sensors that are effective over a broad range of pressures is crucial for the future development of electronic skin applicable to the detection of a wide pressure range from acoustic wave to dynamic human motion. Here, we present flexible capacitive pressure sensors that incorporate micropatterned pyramidal ionic gels to enable ultrasensitive pressure detection. Our devices show superior pressure-sensing performance, with a broad sensing range from a few pascals up to 50 kPa, with fast response times of <20 ms and a low operating voltage of 0...
March 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28240854/correction-to-thermoresponsive-polymer-micropatterns-fabricated-by-dip-pen-nanolithography-for-a-highly-controllable-substrate-with-potential-cellular-applications
#14
Stacey Laing, Raffaella Suriano, Dimitrios A Lamprou, Carol-Anne Smith, Matthew J Dalby, Samuel Mabbott, Karen Faulds, Duncan Graham
No abstract text is available yet for this article.
February 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28226459/a-simple-method-for-micropatterning-nanofibrous-hydrogel-film
#15
K Higashi, N Miki, K Higashi, N Miki, K Higashi, N Miki
This paper propose a new fabrication process for micropatterning a nanofibrous thin film made of bacterial cellulose(BC). BC is a hydrogel produced by specific bacteria and composed of pure cellulosic nanoflbers exhibiting 3D network structure. Such nanofibrous structure is found to be appropriate for adhesion of anchorage-dependent cells. Furthermore, BC shows high biocompatibility and mechanical toughness. Thus, the microfabrication technique for BC broadens potentials for applications. In this study, we report a new method for micropatterning BC film with feature resolution comparable with photolithography technology...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28224240/simulation-of-cell-substrate-traction-force-dynamics-in-response-to-soluble-factors
#16
Tao Liu
Finite element (FE) simulations of contractile responses of vascular muscular thin films (vMTFs) and endothelial cells resting on an array of microposts under stimulation of soluble factors were conducted in comparison with experimental measurements reported in the literature. Two types of constitutive models were employed in the simulations, i.e. smooth muscle cell type and non-smooth muscle cell type. The time histories of the effects of soluble factors were obtained via calibration against experimental measurements of contractile responses of tissues or cells...
February 21, 2017: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/28213499/geometry-and-network-connectivity-govern-the-mechanics-of-stress-fibers
#17
Elena Kassianidou, Christoph A Brand, Ulrich S Schwarz, Sanjay Kumar
Actomyosin stress fibers (SFs) play key roles in driving polarized motility and generating traction forces, yet little is known about how tension borne by an individual SF is governed by SF geometry and its connectivity to other cytoskeletal elements. We now address this question by combining single-cell micropatterning with subcellular laser ablation to probe the mechanics of single, geometrically defined SFs. The retraction length of geometrically isolated SFs after cutting depends strongly on SF length, demonstrating that longer SFs dissipate more energy upon incision...
March 7, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28207187/a-robust-method-to-generate-mechanically-anisotropic-vascular-smooth-muscle-cell-sheets-for-vascular-tissue-engineering
#18
Daniel E Backman, Bauer L LeSavage, Shivem B Shah, Joyce Y Wong
In arterial tissue engineering, mimicking native structure and mechanical properties is essential because compliance mismatch can lead to graft failure and further disease. With bottom-up tissue engineering approaches, designing tissue components with proper microscale mechanical properties is crucial to achieve the necessary macroscale properties in the final implant. This study develops a thermoresponsive cell culture platform for growing aligned vascular smooth muscle cell (VSMC) sheets by photografting N-isopropylacrylamide (NIPAAm) onto micropatterned poly(dimethysiloxane) (PDMS)...
February 16, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28198854/two-photon-polymerization-of-microstructures-by-a-non-diffraction-multifoci-pattern-generated-from-a-superposed-bessel-beam
#19
Liang Yang, Dongdong Qian, Chen Xin, Zhijiang Hu, Shengyun Ji, Dong Wu, Yanlei Hu, Jiawen Li, Wenhao Huang, Jiaru Chu
In this Letter, superposed Bessel beams (SBBs) are realized by alternatively imprinting holograms of opposite-order Bessel beams along the radial direction on a spatial light modulator. The propagation invariance and non-rotation properties of SBBs are theoretically predicted and experimentally demonstrated. The focusing property of SBBs with a high numerical aperture (NA) objective is investigated with the Debye vectorial diffraction theory. Near the focal plane, a circularly distributed multiple foci pattern is achieved...
February 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28195834/surface-acoustic-waves-induced-micropatterning-of-cells-in-gelatin-methacryloyl-gelma-hydrogels
#20
Shahid M Naseer, Amir Manbachi, Mohamadmahdi Samandari, Philipp Walch, Yuan Gao, Yu Shrike Zhang, Farideh Davoudi, Wesley Wang, Karen Abrinia, Jonathan M Cooper, Ali Khademhosseini, Su Ryon Shin
Acoustic force patterning is an emerging technology that provides a platform to control the spatial location of cells in a rapid, accurate, yet contactless manner. However, very few studies have been reported on the usage of acoustic force patterning for the rapid arrangement of biological objects, such as cells, in a three-dimensional (3D) environment. In this study, we report on a bio-acoustic force patterning technique, which uses surface acoustic waves (SAWs) for the rapid arrangement of cells within an extracellular matrix-based hydrogel such as gelatin methacryloyl (GelMA)...
February 14, 2017: Biofabrication
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