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Oju Jeon, Keewon Lee, Eben Alsberg
Growth factors are potent stimuli for regulating cell function in tissue engineering strategies, but spatially patterning their presentation in 3D in a facile manner using a single material is challenging. Micropatterning is an attractive tool to modulate the cellular microenvironment with various biochemical and physical cues and study their effects on stem cell behaviors. Implementing heparin's ability to immobilize growth factors, dual-crosslinkable alginate hydrogels are micropatterned in 3D with photocrosslinkable heparin substrates with various geometries and micropattern sizes, and their capability to establish 3D micropatterns of growth factors within the hydrogels is confirmed...
May 21, 2018: Small
Gergő Fülöp, Mario Brameshuber, Andreas M Arnold, Gerhard J Schütz, Eva Sevcsik
The organization and dynamics of proteins and lipids in the plasma membrane, and their role in membrane functionality, have been subject of a long-lasting debate. Specifically, it is unclear to what extent membrane proteins are affected by their immediate lipid environment and vice versa. Studies on model membranes and plasma membrane vesicles indicated preferences of proteins for lipid phases characterized by different acyl chain order; however, whether such phases do indeed exist in live cells is still not known...
May 17, 2018: Biomolecules
Deteng Zhang, Sai Wu, Jianyong Feng, Yiyuan Duan, Dongming Xing, Changyou Gao
The interplay of microstructures and biological cues is critical to regulate the behaviors of Schwann cells (SCs) in terms of cellular spatial arrangement and directional migration as well as neurite orientation for bridging the proximal and distal stumps of the injured peripheral nervous system. In this study, stripe micropatterns having ridges/grooves of width 20/20 and 20/40 μm were fabricated on the surface of maleimide-functionalized biodegradable poly(ester carbonate) (P(LLA-MTMC)) films by the polydimethylsiloxane mold-pressing method, respectively...
May 13, 2018: Acta Biomaterialia
Borui Xu, Ziao Tian, Jiao Wang, Heetak Han, Taeyoon Lee, Yongfeng Mei
Nanomembrane rolling offers advanced three-dimensional (3D) mesostructures in electronics, optics, and biomedical applications. We demonstrate a high-density and on-chip array of rolled-up nanomembrane actuators with stimuli-responsive function based on the volume expansion of palladium in hydrogen milieu. The uniform stimuli-responsive behavior of high-density nanomembrane rolls leads to huge macroscopic visual detection with more than 50% transmittance change under optimization of micropattern design. The reversible shape changing between rolled and flat (unrolled) statuses can be well explained on the basis of the elastic mechanical model...
April 2018: Science Advances
Abir Ghosh, Dipankar Bandyopadhyay, Ashutosh Sharma
Elastic contact lithography (ECL) and electric field lithography (EFL) have both shown significant potential to develop large-area micropatterns on polymeric surfaces. Recently, the major challenges associated with these processes have been the improvement of the aspect ratio and reduction in the size and periodicity of the patterns fabricated. Herein, with the help of non-linear simulations, we show that combining these methods can be one recipe to overcome these limitations. We consider a linear viscoelastic film for the linear and non-linear analyses...
May 8, 2018: Soft Matter
Bibhas Roy, Saradha Venkatachalapathy, Prasuna Ratna, Yejun Wang, Doorgesh Sharma Jokhun, Mallika Nagarajan, G V Shivashankar
Cells in tissues undergo transdifferentiation programs when stimulated by specific mechanical and biochemical signals. While seminal studies have demonstrated that exogenous biochemical factors can reprogram somatic cells into pluripotent stem cells, the critical roles played by mechanical signals in such reprogramming process have not been well documented. In this paper, we show that laterally confined growth of fibroblasts on micropatterned substrates induces nuclear reprogramming with high efficiency in the absence of any exogenous reprogramming factors...
May 7, 2018: Proceedings of the National Academy of Sciences of the United States of America
Waqas Waheed, Anas Alazzam, Bobby Mathew, Nicolas Christoforou, Eiyad Abu-Nada
This short communication introduces a continuous-flow, dielectrophoresis-based lateral fluid flow fractionation microdevice for detection/isolation of circulating tumor cells in the presence of other haematological cells. The device utilizes two sets of planar interdigitated transducer electrodes micropatterned on top of a glass wafer using standard microfabrication techniques. A microchannel with a single inlet and two outlets, realized in polydimethylsiloxane, is bonded on the glass substrate. The two sets of electrodes slightly protrude into the microchannel...
April 30, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Florian A Gegenfurtner, Berenice Jahn, Helga Wagner, Christoph Ziegenhain, Wolfgang Enard, Ludwig Geistlinger, Joachim O Rädler, Angelika M Vollmar, Stefan Zahler
Developmental processes such as angiogenesis are associated to a constant remodeling of the actin cytoskeleton in response to different mechanical stimuli. The mechanosensitive transcription factors MRTF and YAP are important mediators of this challenging adaptation process. However, it is yet unknown whether both pathways respond identical or divergent to a given microenvironmental guidance cue. Here, we use a micropatterning approach to dissect single aspects of cellular behavior in a spatiotemporally controllable setting...
May 3, 2018: Journal of Cell Science
Jordi Cools, Qianru Jin, Eugene Yoon, Diego Alba Burbano, Zhenxiang Luo, Dieter Cuypers, Geert Callewaert, Dries Braeken, David H Gracias
Microelectrode arrays (MEAs) have proved to be useful tools for characterizing electrically active cells such as cardiomyocytes and neurons. While there exist a number of integrated electronic chips for recording from small populations or even single cells, they rely primarily on the interface between the cells and 2D flat electrodes. Here, an approach that utilizes residual stress-based self-folding to create individually addressable multielectrode interfaces that wrap around the cell in 3D and function as an electrical shell-like recording device is described...
April 2018: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
Zengjun Jin, Mengyue Nie, Ran Hu, Tianhu Zhao, Jingyue Xu, Dongwei Chen, Juanli Yun, Luyan Z Ma, Wenbin Du
Bacterial biofilms play essential roles in biogeochemical cycling, degradation of environmental pollutants, infection diseases, and maintenance of host health. The lack of quantitative methods for growing and characterizing biofilms remains a major challenge in understanding biofilm development. In this study, a dynamic sessile-droplet habitat is introduced, a simple method which cultivates biofilms on micropatterns with diameters of tens to hundreds of micrometers in a microfluidic channel. Nanoliter plugs are utilized, spaced by immiscible carrier oil to initiate and support the growth of an array of biofilms, anchored on and spatially confined to the micropatterns arranged on the bottom surface of the microchannel, while planktonic or dispersal cells are flushed away by shear force of aqueous plugs...
May 2, 2018: Small
Yifan Li, Meng Su, Zheng Li, Zhandong Huang, Fengyu Li, Qi Pan, Wanjie Ren, Xiaotian Hu, Yanlin Song
The precise integration of microscale dots and lines with controllable interfacing connections is highly important for the fabrication of functional devices. To date, the solution-processible methods are used to fabricate the heterogeneous micropatterns for different materials. However, for increasingly miniaturized and multifunctional devices, it is extremely challenging to engineer the uncertain kinetics of a solution on the microstructures surfaces, resulting in uncontrollable interface connections and poor device performance...
April 30, 2018: Small
Jiao Yang Lu, Xin Xing Zhang, Qiu Yan Zhu, Fu Rui Zhang, Wei Tao Huang, Xue Zhi Ding, Li Qiu Xia, Hong Qun Luo, Nian Bing Li
Patterning graphene allows to precisely tune its properties to manufacture flexible functional materials or miniaturized devices for electronic and biomedical applications. However, conventional lithographic techniques are cumbersome for scalable producing time- and cost-effective graphene patterns, thus greatly impeding their practical applications. Here we present a simple scalable fabrication of wafer-scale 3D graphene micropatterns by direct laser tuning expansion and reduction of graphene oxide using a standard LightScribe DVD burner...
April 27, 2018: ACS Applied Materials & Interfaces
Noah B Schorr, Annie G Jiang, Joaquín Rodríguez-López
Addressing challenges in interfacial electrochemistry requires multimodal approaches that correlate the local structure and reactivity of materials with high spatial and temporal versatility. Here, we introduce spatiotemporally correlated Raman spectroscopy and scanning electrochemical microscopy (SECM) to study the impact that structural heterogeneities, interfa-cial decomposition products, and layer number have on the electron transfer properties of graphene electrodes. By co-localizing the SECM probe and laser line, we successfully obtained congruent SECM and Raman images at a rate 5 s per pixel with sub 10 µm resolution, obtaining full spectra per pixel at a signal-to-noise ratio as high as ~20...
April 27, 2018: Analytical Chemistry
Alwin Daus, Cristina Roldán-Carmona, Konrad Domanski, Stefan Knobelspies, Giuseppe Cantarella, Christian Vogt, Michael Grätzel, Mohammad Khaja Nazeeruddin, Gerhard Tröster
Metal-halide perovskites have emerged as promising materials for optoelectronics applications, such as photovoltaics, light-emitting diodes, and photodetectors due to their excellent photoconversion efficiencies. However, their instability in aqueous solutions and most organic solvents has complicated their micropatterning procedures, which are needed for dense device integration, for example, in displays or cameras. In this work, a lift-off process based on poly(methyl methacrylate) and deep ultraviolet lithography on flexible plastic foils is presented...
April 25, 2018: Advanced Materials
Lisha Zheng, Jingyi Jiang, Jinpeng Gui, Lingyu Zhang, Xiaoyi Liu, Yan Sun, Yubo Fan
The periodontal ligament (PDL) is highly ordered connective tissue located between the alveolar bone and cementum. An aligned and organized architecture is required for its physiological function. We applied micropatterning technology to arrange PDL cells in 10- or 20-μm-wide extracellular protein patterns. Cell and nuclear morphology, cytoskeleton, proliferation, differentiation, and matrix metalloproteinase system expression were investigated. Micropatterning clearly elongated PDL cells with a low cell-shape index and low spreading area...
April 24, 2018: Biophysical Journal
Yujeong Lee, Kang Lib Kim, Han Sol Kang, Beomjin Jeong, Chanho Park, Insung Bae, Seok Ju Kang, Youn Jung Park, Cheolmin Park
Epitaxial crystallization of thin poly(vinylidene fluoride-co-trifluoroethylene) (PVDF-TrFE) films is important for the full utilization of their ferroelectric properties. Epitaxy can offer a route for maximizing the degree of crystallinity with the effective orientation of the crystals with respect to the electric field. Despite various approaches for the epitaxial control of the crystalline structure of PVDF-TrFE, its epitaxy on a semiconductor is yet to be accomplished. Herein, the epitaxial growth of PVDF-TrFE crystals on a single-crystalline organic semiconductor rubrene grown via physical vapor deposition is presented...
April 24, 2018: Small
Christina L Rettinger, Kai P Leung, Rodney K Chan
Adipose-derived stem cells are capable of self-renewal and differentiation along multiple cell lineages, and have potential applications in a wide range of therapies. ASCs are commonly cultured as monolayers on tissue culture plastic, but there are indications that they may lose their cell-specific properties with time in vitro. There has been a growing interest in culturing adherent cells using three-dimensional techniques based on the understanding that growing cells on plastic surfaces cannot truly recapitulate 3D in vivo conditions...
2018: Methods in Molecular Biology
Yu Lei, Xianyun Zhang, Dingding Xu, Minfeng Yu, Zhiran Yi, Zhixiang Li, Aihua Sun, Gaojie Xu, Ping Cui, Jianjun Guo
Micro- and nanopatterning of cost-effective addressable metallic nanostructures has been a long endeavor in terms of both scientific understanding and industrial needs. Herein, a simple and efficient dynamic meniscus-confined electrodeposition (MCED) technique for precisely positioned copper line micropatterns with superior electrical conductivity (greater than 1.57 × 104 S/cm) on glass, silicon, and gold substrates is reported. An unexpected higher printing speed in the evaporative regime is realized for precisely positioned copper lines patterns with uniform width and height under horizontal scanning-mode...
April 24, 2018: Journal of Physical Chemistry Letters
Kang Zhou, Hari Krishna Bisoyi, Jian-Qiu Jin, Cong-Long Yuan, Zhen Liu, Dong Shen, Yan-Qing Lu, Zhi-Gang Zheng, Weian Zhang, Quan Li
Self-organized stimuli-responsive smart materials with adjustable attributes are highly desirable for a plethora of device applications. Simple cubic lattice is quite uncommon in soft condensed matter due to its lower packing factor. Achieving a stable simple cubic soft lattice and endowing such a lattice with dynamic reconstruction capability solely by a facile light irradiation are of paramount significance for both fundamental studies and engineering explorations. Herein, an elegant stable self-organized simple cubic soft lattice, i...
April 23, 2018: Advanced Materials
Cancan Zhao, Xiaoya Wang, Long Gao, Linguo Jing, Quan Zhou, Jiang Chang
The micro/nano hybrid structure is considered to be a biomaterial characteristic to stimulate osteogenesis by mimicking the three-dimensional structure of the bone matrix. However, the mechanism of the hybrid structure induced osteogenic differentiation of stem cells is still unknown. For elucidating the mechanisms, one of the challenge is to directly fabricate micro/nano hybrid structure on bioceramics because of its brittleness. In this study, hydroxyapatite (HA) bioceramics with the micro/nano hybrid structure were firstly fabricated via a hydrothermal treatment and template method, and the effect of the different surface structures on the expression of integrins, BMP2 signaling pathways and cell-cell communication was investigated...
April 17, 2018: Acta Biomaterialia
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