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Youngjae Chun, Colin P Kealey, Daniel S Levi, David A Rigberg, Yanfei Chen, Bryan W Tillman, K P Mohanchandra, Mahdis Shayan, Gregory P Carman
Sputter-deposited thin film nitinol constructs with various micropatterns were fabricated to evaluate their effect on the vessel wall in vivo when used as a covering for commercially available stents. Thin film nitinol constructs were used to cover stents and deployed in non-diseased swine arteries. Swine were sacrificed after approximately four weeks and the thin film nitinol-covered stents were removed for histopathologic evaluation. Histopathology revealed differences in neointimal thickness that correlated with the thin film nitinol micropattern...
December 8, 2016: Journal of Biomaterials Applications
Chunlei Zhu, Jiajia Xue, Kyle D Gilroy, Da Huo, Song Shen, Younan Xia
This paper describes a simple method for the fabrication of micropatterned polymer nanorod forests by templating against the channels in an anodized aluminum oxide membrane partially masked by gelatin. The nanorod forests easily support bimodal drug loading, with one drug encapsulated in the nanorods and the other physisorbed on their surface. During cell culture, preosteoblasts are predominantly attracted to the nanorod forests and driven to climb up along the nanorods. This type of scaffold integrates both microscale and nanoscale features into a single substrate, holding great potential for applications in cell culture and tissue engineering...
December 8, 2016: ACS Applied Materials & Interfaces
Seok Min Yoon, Jun Heuk Park, Bartosz A Grzybowski
Freestanding MOF films up to six-inches across and replicating various surface (micro)patterns are prepared via a templated growth method. When grown on copper supports, these films have preferred orientation of the constituent crystallites, translating into markedly different wetting properties of the film's two surfaces (water-pinning vs. water repellant). In addition, the films exhibit differential sorption of various organic solvents, can recover oil spills from seawater, and can also act as active layers of chemical sensors...
November 30, 2016: Angewandte Chemie
Julia Bruchmann, Ivana Pini, Tejeshwar S Gill, Thomas Schwartz, Pavel A Levkin
Biofilms represent an immense problem in medicine due to their strong drug-resistant properties and inherent stress-response activities. Due to the inhomogeneous and very complex architectures of large biofilm aggregates, biofilm studies often suffer from low reproducibility. In this study, an approach to form arrays of homogeneous biofilm microclusters with defined 2D geometries is presented. The method is based on the formation of water-infused hydrophilic porous polymer areas with precise geometries separated by "slippery" lubricant-infused porous surface (SLIPS)...
November 23, 2016: Advanced Healthcare Materials
Gregory T Carroll, Jeffrey R Lancaster, Nicholas J Turro, Jeffrey T Koberstein, Angela Mammana
This report demonstrates the electroless deposition of Ni onto micropatterns of poly (acrylic acid) (PAA) photografted to phthalimide-terminated self-assembled monolayers (SAMs). PAA is spin-coated onto phthalimide SAMs and covered with a photomask. UV irradiation selectively binds PAA to exposed regions of the surface, allowing PAA on unexposed regions to be rinsed off. A Pd catalyst is then selectively adsorbed to regions of the surface where PAA is bound. The adsorbed catalyst selectively initiates Ni plating upon immersion of the substrate into a Ni(SO4 ) bath...
November 22, 2016: Macromolecular Rapid Communications
Yuichi Furuhata, Yuka Kikuchi, Shunsuke Tomita, Keitaro Yoshimoto
Wound-healing factors secreted from mesenchymal stem cells (MSCs) modulate the immune response and facilitate proliferation of neighboring cells. Although in vitro three-dimensional (3D) culture techniques have improved the therapeutic potential of MSCs, no studies have focused on the effects of cell aggregation alone. In this study, the effect of cell aggregation on the up-regulation of wound-healing proteins secretions was investigated by constructing small spheroids of human adipose-derived stem cells (hADSCs) on a micropatterned surface...
November 9, 2016: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
Jingting Li, Fusheng Zhao, Wei-Chuan Shih
We report a novel patterning technique to direct-write microscale nanoporous gold (NPG) features by projecting laser patterns using a spatial light modulator (SLM) onto an Au/Ag alloy film immersed in diluted nitric acid solutions. Heat accumulation induced by the photothermal effect enables localized dealloying in such solutions, which is otherwise impotent at room temperature. Consequently, NPG micropatterns are formed at the irradiated spots while the surrounding alloy remains intact. We have studied the size of the patterned NPG microstructures with respect to laser power and irradiation time...
October 3, 2016: Optics Express
Philip Gröger, Nicole Poulsen, Jennifer Klemm, Nils Kröger, Michael Schlierf
The intricate, genetically controlled biosilica nano- and micropatterns produced by diatoms are a testimony for biology's ability to control mineral formation (biomineralization) at the nanoscale and regarded as paradigm for nanotechnology. Previously, several protein families involved in diatom biosilica formation have been identified, and many of them remain tightly associated with the final biosilica structure. Determining the locations of biosilica-associated proteins with high precision is, therefore expected to provide clues to their roles in biosilica morphogenesis...
November 9, 2016: Scientific Reports
Caterina Cristallini, Elisa Cibrario Rocchietti, Mariacristina Gagliardi, Leonardo Mortati, Silvia Saviozzi, Elena Bellotti, Valentina Turinetto, Maria Paola Sassi, Niccoletta Barbani, Claudia Giachino
The biomaterial scaffold plays a key role in most tissue engineering strategies. Its surface properties, micropatterning, degradation, and mechanical features affect not only the generation of the tissue construct in vitro, but also its in vivo functionality. The area of myocardial tissue engineering still faces significant difficulties and challenges in the design of bioactive scaffolds, which allow composition variation to accommodate divergence in the evolving myocardial structure. Here we aimed at verifying if a microstructured bioartificial scaffold alone can provoke an effect on stem cell behavior...
2016: Stem Cells International
Leo Q Wan, Amanda S Chin, Kathryn E Worley, Poulomi Ray
Increasing evidence suggests that intrinsic cell chirality significantly contributes to the left-right (LR) asymmetry in embryonic development, which is a well-conserved characteristic of living organisms. With animal embryos, several theories have been established, but there are still controversies regarding mechanisms associated with embryonic LR symmetry breaking and the formation of asymmetric internal organs. Recently, in vitro systems have been developed to determine cell chirality and to recapitulate multicellular chiral morphogenesis on a chip...
December 19, 2016: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
Samuel Martin, Bharat Bhushan
Superoleophobic surfaces that exhibit self-cleaning, antifouling, low-drag, and anti-smudge properties with high transparency are of interest in industrial applications including optical devices, solar panels, and self-cleaning windows. In many superoleophobic surfaces created to date, the lack of mechanical durability has been an issue. In this work, for the first time, transparent, wear-resistant, superhydrophobic and superoleophobic surfaces were developed for polydimethylsiloxane (PDMS) using a simple and scalable fabrication technique...
February 15, 2017: Journal of Colloid and Interface Science
Angie M Y Shum, Hui Che, Andy On-Tik Wong, Chenzi Zhang, Hongkai Wu, Camie W Y Chan, Kevin Costa, Michelle Khine, Chi-Wing Kong, Ronald A Li
A novel cardiomimetic biohybrid material, termed as the human ventricular cardiac anisotropic sheet (hvCAS) is reported. Well-characterized human pluripotent stem cell-derived ventricular cardiomyocytes were strategically aligned to reproduce key electrophysiological features of native human ventricle, which, along with specific selection criteria, allows for a direct visualization of arrhythmic spiral re-entry and represents a revolutionary tool to assess preclinical drug-induced arrhythmogenicity.
November 2, 2016: Advanced Materials
Wanxin Shi, Tailin Xu, Li-Ping Xu, Yanxia Chen, Yongqiang Wen, Xueji Zhang, Shutao Wang
We demonstrate a simple and environment-friendly strategy to fabricate cell micropatterns on a nanodendritic superhydrophilic silica substrate separated by silicone-oil-modified superhydrophobic barriers. The superhydrophilic spots exhibit excellent cell adhesion capability due to the enhanced local topographic interaction between cell filopodia and the nanodendritic substrate interface, and result in sensational cell micropatterns. In contrast, the anti-adhesion of silicone-oil-modified superhydrophobic barriers prevents cell migration and results in long-term cell-repellency...
November 1, 2016: Nanoscale
Kalpana Mandal, Atef Asnacios, Bruno Goud, Jean-Baptiste Manneville
The mechanical properties of cells impact on their architecture, their migration, intracellular trafficking, and many other cellular functions and have been shown to be modified during cancer progression. We have developed an approach to map the intracellular mechanical properties of living cells by combining micropatterning and optical tweezers-based active microrheology. We optically trap micrometer-sized beads internalized in cells plated on crossbow-shaped adhesive micropatterns and track their displacement following a step displacement of the cell...
November 15, 2016: Proceedings of the National Academy of Sciences of the United States of America
Maya Shelly, Seong-Ii Lee, Giulia Suarato, Yizhi Meng, Sophie Pautot
Protein micropatterning techniques, including microfluidic devices and protein micro-contact printing, enable the generation of highly controllable substrates for spatial manipulation of intracellular and extracellular signaling determinants to examine the development of cultured dissociated neurons in vitro. In particular, culture substrates coated with proteins of interest in defined stripes, including cell adhesion molecules and secreted proteins, have been successfully used to study neuronal polarization, a process in which the neuron establishes axon and dendrite identities, a critical architecture for the input/output functions of the neuron...
2017: Methods in Molecular Biology
H Yamamoto, R Matsumura, H Takaoki, S Katsurabayashi, A Hirano-Iwata, M Niwano
The structure and connectivity of cultured neuronal networks can be controlled by using micropatterned surfaces. Here, we demonstrate that the direction of signal propagation can be precisely controlled at a single-cell resolution by growing primary neurons on micropatterns. To achieve this, we first examined the process by which axons develop and how synapses form in micropatterned primary neurons using immunocytochemistry. By aligning asymmetric micropatterns with a marginal gap, it was possible to pattern primary neurons with a directed polarization axis at the single-cell level...
July 25, 2016: Applied Physics Letters
Fred Etoc, Jakob Metzger, Albert Ruzo, Christoph Kirst, Anna Yoney, M Zeeshan Ozair, Ali H Brivanlou, Eric D Siggia
The earliest aspects of human embryogenesis remain mysterious. To model patterning events in the human embryo, we used colonies of human embryonic stem cells (hESCs) grown on micropatterned substrate and differentiated with BMP4. These gastruloids recapitulate the embryonic arrangement of the mammalian germ layers and provide an assay to assess the structural and signaling mechanisms patterning the human gastrula. Structurally, high-density hESCs localize their receptors to transforming growth factor β at their lateral side in the center of the colony while maintaining apical localization of receptors at the edge...
November 7, 2016: Developmental Cell
Elena Scarpa, Alice Roycroft, Eric Theveneau, Emmanuel Terriac, Matthieu Piel, Roberto Mayor
No abstract text is available yet for this article.
October 15, 2016: Biology Open
Katrin Niegelhell, Michael Suessenbacher, Katrin Jammernegg, Thomas Ganner, Daniel Schwendenwein, Helmut Schwab, Franz Stelzer, Harald Plank, Stefan Spirk
The creation of nano- and micropatterned polymer films is a crucial step for innumerous applications in science and technology. However, there are several problems associated with environmental aspects concerning the polymer synthesis itself, crosslinkers to induce the patterns as well as toxic solvents used for the preparation and even more important development of the films (e.g. chlorobenzene). In this paper, we present a facile method to produce micro- and nanopatterned biopolymer thin films using enzymes as so called biodevelopers...
October 13, 2016: Biomacromolecules
Alessia Deglincerti, Fred Etoc, M Cecilia Guerra, Iain Martyn, Jakob Metzger, Albert Ruzo, Mijo Simunovic, Anna Yoney, Ali H Brivanlou, Eric Siggia, Aryeh Warmflash
Fate allocation in the gastrulating embryo is spatially organized as cells differentiate into specialized cell types depending on their positions with respect to the body axes. There is a need for in vitro protocols that allow the study of spatial organization associated with this developmental transition. Although embryoid bodies and organoids can exhibit some spatial organization of differentiated cells, methods that generate embryoid bodies or organoids do not yield consistent and fully reproducible results...
November 2016: Nature Protocols
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