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https://www.readbyqxmd.com/read/29145019/ordered-cylindrical-micropatterned-petri-dishes-used-as-scaffolds-for-cell-growth
#1
Van-Tien Bui, Le Thi Thuy, Joon Sig Choi, Ho-Suk Choi
Three-dimensional (3D) culture dish patterned with a microwell structure demonstrates a great application potential in biotechnology. This study reports on the easy fabrication of an ordered customizable honeycomb microwell array on the surface of polymer substrates including the commercial Petri dish to create a biological platform for cell culture. The fabrication method is based on a very simple solvent dip-coating technique and the methanol accumulation-induced phase separation in which a binary mixture of chloroform and methanol is used to induce a ternary solution and to guarantee the formation of the ordered pore array on the substrate...
November 10, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29144124/ionic-liquid-microstrips-impregnated-with-magnetic-nano-stirrers-for-sensitive-gas-sensors
#2
Richard Gondosiswanto, David Brynn Hibbert, Yu Fang, Chuan Zhao
Ionic liquids have been regarded as promising electrolytes as substitutes for volatile aqueous or organic solvents for electrochemical gas sensors. However ionic liquids are viscous and the slow diffusion of gas molecules leads to poor sensitivity and sluggish response times. Herein, we describe a strategy using an array of microstrips of ionic liquid containing magnetic nanoparticles as nano-stirrers for enhanced mass transport and gas sensing. Magnetic CoFe2O4 nanoparticles are synthesized and dispersed in a hydrophobic ionic liquid [BMP][Ntf2]...
November 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29140569/single-cell-functional-analysis-of-stem-cell-derived-cardiomyocytes-on-micropatterned-flexible-substrates
#3
Jan David Kijlstra, Dongjian Hu, Peter van der Meer, Ibrahim J Domian
Human pluripotent stem-cell derived cardiomyocytes (hPSC-CMs) hold great promise for applications in human disease modeling, drug discovery, cardiotoxicity screening, and, ultimately, regenerative medicine. The ability to study multiple parameters of hPSC-CM function, such as contractile and electrical activity, calcium cycling, and force generation, is therefore of paramount importance. hPSC-CMs cultured on stiff substrates like glass or polystyrene do not have the ability to shorten during contraction, making them less suitable for the study of hPSC-CM contractile function...
November 15, 2017: Current Protocols in Stem Cell Biology
https://www.readbyqxmd.com/read/29131936/nanomechanical-force-mapping-of-restricted-cell-to-cell-collisions-oscillating-between-contraction-and-relaxation
#4
Benhui Hu, Wan Ru Leow, Pingqiang Cai, Yong-Qiang Li, Yun-Long Wu, Xiaodong Chen
Contact-mediated cell migration strongly determines the invasiveness of the corresponding cells, collective migration, and morphogenesis. The quantitative study of cellular response upon contact relies on cell-to-cell collision, which rarely occurs in conventional cell culture. Herein, we developed a strategy to activate a robust cell-to-cell collision within smooth muscle cell pairs. Nanomechanical traction force mapping reveals that the collision process is promoted by the oscillatory modulations between contraction and relaxation and orientated by the filopodial bridge composed of nanosized contractile machinery...
November 16, 2017: ACS Nano
https://www.readbyqxmd.com/read/29131300/engineered-scaffolds-and-cell-based-therapy-for-periodontal-regeneration
#5
Daniela Carmagnola, Mihai Tarce, Claudia Dellavia, Lia Rimondini, Elena M Varoni
BACKGROUND: The main objective of regenerative periodontal therapy is to completely restore the periodontal tissues lost. This review summarizes the most recent evidence in support of scaffold- and cell-based tissue engineering, which are expected to play a relevant role in next-generation periodontal regenerative therapy. METHODS: A literature search (PubMed database) was performed to analyze more recently updated articles regarding periodontal regeneration, scaffolds and cell-based technologies...
November 10, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/29130596/evaluation-of-a-bioengineered-construct-for-tissue-engineering-applications
#6
Perla Ayala, Erbin Dai, Michael Hawes, Liying Liu, Ovijit Chaudhuri, Carolyn A Haller, David J Mooney, Elliot L Chaikof
Effective biomaterial options for tissue repair and regeneration are limited. Current biologic meshes are derived from different tissue sources and are generally sold as decellularized tissues. This work evaluated two collagen based bioengineered constructs and a commercial product in a model of abdominal full thickness defect repair. To prepare the bioengineered construct, collagen type 1 from porcine skin was isolated using an acid solubilization method. After purification, the collagen was formed into collagen sheets that were physically bonded to form a mechanically robust construct that was subsequently laser micropatterned with pores as a means to promote tissue integration (collagen only construct)...
November 11, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29125131/polymer-scaffolds-for-vascular-tissue-engineering-fabricated-by-combined-electrospinning-and-hot-embossing
#7
Shujie Yan, Xiang Zhang, Liguo Zhang, Hao Liu, Xiaofeng Wang, Qian Li
Although tissue engineering has shown great advances in recent years, creating proper mechanical properties and cell growth microenvironments is still challenging. In this study, electrospun nanofibrous membranes were hot embossed to develop three-dimensional hierarchical micro/nanostructures that load and culture human umbilical-vein endothelial cells (HUVECs). The hot-embossed membranes exhibited not only superior mechanical properties (the tensile strength was 7.01 ± 0.18 MPa and the tensile modulus was 166...
November 10, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/29122711/developmental-refinement-of-synaptic-transmission-on-micropatterned-single-layer-graphene
#8
Sandeep Keshavan, Shovan Naskar, Alberto Diaspro, Laura Cancedda, Silvia Dante
Interfacing neurons with a single atomic layer of graphene, single atomic layer of sp2 hybridized C-atoms, is a key paradigm in understanding how to exploit the unique properties of such a two-dimensional system for neural prosthetics and biosensors development. In order to fabricate graphene-based circuitry, a reliable large area patterning method is a requirement. Following a previously developed protocol, we monitored the in vitro neuronal development of geometrically ordered neural network growing onto patterned Single Layer Graphene (SLG) coated with poly-D-lysine...
November 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29121452/3d-maskless-micropatterning-for-regeneration-of-highly-organized-tubular-tissues
#9
Chi Ma, Tiejun Qu, Bei Chang, Yan Jing, Jerry Q Feng, Xiaohua Liu
Micropatterning is a widely used powerful tool to create highly ordered microstructures on material surfaces. However, due to technical limitations, the integration of micropatterned microstructures into bioinspired 3D scaffolds to successfully regenerate well-organized functional tissues is not achieved. In this work, a unique maskless micropatterning technology is reported to create 3D nanofibrous matrices with highly organized tubular architecture for tissue regeneration. This micropatterning method is a laser-guided, noncontact, high-precision, flexible computer programming of machining process that can create highly ordered tubules with the density ranged from 1000 to 60 000 mm(-2) and the size varied from 300 nm to 30 µm in the bioinspired 3D matrix...
November 9, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29120470/modulation-of-cellular-polarization-and-migration-by-ephrin-eph-signal-mediated-boundary-formation
#10
Sahar Javaherian, Elisa D'Arcangelo, Benjamin Slater, Camila Londono, Bin Xu, Alison P McGuigan
Compartment boundaries are essential for ensuring proper cell organization during embryo development and in adult tissues, yet the mechanisms underlying boundary establishment are not completely understood. A number of mechanisms, including (i) differential adhesion, (ii) differential tension, and (iii) cell signaling-mediated cell repulsion, are known to contribute and likely a context-dependent balance of each of these dictates boundary implementation. The ephrin/Eph signaling pathway is known to impact boundary formation in higher animals...
November 9, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/29116017/regenerative-potential-of-primary-adult-human-neural-stem-cells-on-micropatterned-bio-implants-boosts-motor-recovery
#11
Carole Davoust, Benjamin Plas, Amélie Béduer, Boris Demain, Anne-Sophie Salabert, Jean Christophe Sol, Christophe Vieu, Laurence Vaysse, Isabelle Loubinoux
BACKGROUND: The adult brain is unable to regenerate itself sufficiently after large injuries. Therefore, hopes rely on therapies using neural stem cell or biomaterial transplantation to sustain brain reconstruction. The aim of the present study was to evaluate the improvement in sensorimotor recovery brought about by human primary adult neural stem cells (hNSCs) in combination with bio-implants. METHODS: hNSCs were pre-seeded on implants micropatterned for neurite guidance and inserted intracerebrally 2 weeks after a primary motor cortex lesion in rats...
November 7, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29114582/engineering-microdent-structures-of-bone-implant-surfaces-to-enhance-osteogenic-activity-in-mscs
#12
Sophia Li, Thomas Chow, Julia Chu
Problems persist with the integration of hip and dental implants with host bone tissues, which may result in long-term implant failure. Previous studies have found that implants bearing irregular surfaces can facilitate osseointegration. An improvement to this approach would use implant surfaces harboring a well-defined surface microstructure to decrease variability in implant surfaces. In this study, we tested whether well-defined surfaces with arrays of microdents (each with depth approximately 3 µm) significantly affected the morphology, proliferation, and osteogenic activity of mesenchymal stem cells (MSCs)...
March 2017: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/29104114/newfound-sex-differences-in-axonal-structure-underlie-differential-outcomes-from-in-vitro-traumatic-axonal-injury
#13
Jean-Pierre Dollé, Andrew Jaye, Stewart A Anderson, Hossein Ahmadzadeh, Vivek B Shenoy, Douglas H Smith
Since traumatic axonal injury (TAI) is implicated as a prominent pathology of concussion, we examined potential sex differences in axon structure and responses to TAI. Rat and human neurons were used to develop micropatterned axon tracts in vitro that were genetically either male or female. Ultrastructural analysis revealed for the first time that female axons were consistently smaller with fewer microtubules than male axons. Computational modeling of TAI showed that these structural differences place microtubules in female axons at greater risk of failure during trauma under the same applied loads than in male axons...
November 13, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/29097768/freeform-micropatterning-of-living-cells-into-cell-culture-medium-using-direct-inkjet-printing
#14
Ju An Park, Sejeong Yoon, Jimin Kwon, Hesung Now, Young Kwon Kim, Woo-Jong Kim, Joo-Yeon Yoo, Sungjune Jung
Microfabrication methods have widely been used to control the local cellular environment on a micron scale. However, accurately mimicking the complexity of the in vivo tissue architecture while maintaining the freedom of form and design is still a challenge when co-culturing multiple types of cells on the same substrate. For the first time, we present a drop-on-demand inkjet printing method to directly pattern living cells into a cell-friendly liquid environment. High-resolution control of cell location is achieved by precisely optimizing printing parameters with high-speed imaging of cell jetting and impacting behaviors...
November 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29082413/a-micropatterned-elastomeric-surface-with-enhanced-frictional-properties-under-wet-conditions-and-its-application
#15
H Ko, M Seong, H E Jeong
Engineered surfaces that have high friction under wet or lubricated conditions are important in many practical applications. However, it is not easy to achieve stable high friction under wet conditions because a layer of fluid prevents direct solid-solid contact. Here, we report a micropatterned elastomeric surface with superior wet friction. The surface has unique arch-shaped microstructures arrayed in a circle on the surface to provide high friction on wet or flooded surfaces. The arch-shaped micropatterned surface exhibits remarkably enhanced and stable friction under wet conditions, surpassing even the performance of the hexagonal patterns of tree frogs, owing to the large contact surface and the optimal shape of drainage channels...
October 30, 2017: Soft Matter
https://www.readbyqxmd.com/read/29082070/angle-selective-optical-filter-for-highly-sensitive-reflection-photoplethysmogram
#16
Chan-Sol Hwang, Sung-Pyo Yang, Kyung-Won Jang, Jung-Woo Park, Ki-Hun Jeong
We report an angle-selective optical filter (ASOF) for highly sensitive reflection photoplethysmography (PPG) sensors. The ASOF features slanted aluminum (Al) micromirror arrays embedded in transparent polymer resin, which effectively block scattered light under human tissue. The device microfabrication was done by using geometry-guided resist reflow of polymer micropatterns, polydimethylsiloxane replica molding, and oblique angle deposition of thin Al film. The angular transmittance through the ASOF is precisely controlled by the angle of micromirrors...
October 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/29063084/a-simple-culture-system-for-long-term-imaging-of-individual-c-elegans
#17
William E Pittman, Drew B Sinha, William B Zhang, Holly E Kinser, Zachary Pincus
We have miniaturized standard culture techniques to rear arrays of isolated, individual C. elegans throughout their lives on solid gel media. The resulting apparatus is compatible with brightfield and fluorescence microscopy, enabling longitudinal studies of morphology and fluorescent transgene expression. Our culture system exploits a novel crosslinking reaction between a polyethylene glycol hydrogel and a silicone elastomer to constrain animals to individual "corrals" on the gel surface. These devices are simple to construct on the benchtop with commercially available reagents, and, unlike microfluidic isolation methods, do not rely on micropatterned materials...
November 7, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29059516/electrospun-micropatterned-nanocomposites-incorporated-with-cu2s-nanoflowers-for-skin-tumor-therapy-and-wound-healing
#18
Xiaocheng Wang, Fang Lv, Tian Li, Yiming Han, Zhengfang Yi, Mingyao Liu, Jiang Chang, Chengtie Wu
Surgical excision of skin cancers can hardly remove the tumor tissues completely and simultaneously result in cutaneous defects. To avoid tumor recurrence and heal the tumor-induced wounds, we designed a tissue engineering membranes possessing bifunctions of tumor therapy and skin tissue regeneration. The micropatterned nanocomposite membrane was successfully fabricated by incorporating Cu2S nanoflowers into biopolymer fibers via a modified electrospinning method. With uniformly-embedded Cu2S nanoparticles, the membranes exhibited excellent and controllable photothermal performance under near-infrared (NIR) irradiation, which resulted in high mortality (> 90 %) of skin tumor cells and effectively inhibited tumor growth in mice...
October 23, 2017: ACS Nano
https://www.readbyqxmd.com/read/29050528/uv-assisted-3d-bioprinting-of-nano-reinforced-hybrid-cardiac-patch-for-myocardial-tissue-engineering
#19
Mohammad Izadifar, Dean Chapman, Paul Babyn, Xiongbiao Chen, Michael E Kelly
Biofabrication of cell supportive cardiac patches that can be directly implanted on myocardial infarct is a potential solution for myocardial infarction repair. Ideally, cardiac patches should be able to mimic myocardium extracellular matrix for rapid integration with the host tissue, raising the need to develop cardiac constructs with complex features. In particular, cardiac patches should be electrically conductive, mechanically robust and elastic, biologically active and pre-vascularized.. In this study, we aim to biofabricate a nano-reinforced hybrid cardiac patch laden with human coronary artery endothelial cells (HCAECs) with improved electrical, mechanical and biological behavior...
October 19, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29049882/plasmonic-nanochemistry-based-on-nanohole-array
#20
Bin Ai, Zengyao Wang, Helmuth Möhwald, Gang Zhang
We show that the growth of Ag nanoparticles (NPs) follows the areas of maximum plasmonic field in nanohole arrays (NAs). We thus obtain Ag NP rings not connected to the metallic rim of the nanoholes. The photocatalytic effect resulting from the enhanced E-field of NAs boosts the reaction and is responsible for the site selectivity. The strategy, using plasmonics to control a chemical reaction, can be expanded to organic reactions, for example, synthesis of polypyrrole. After the NA film is removed, ordered ring-shaped Ag NPs are easily obtained, inspiring a facile micropatterning method...
October 23, 2017: ACS Nano
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