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https://www.readbyqxmd.com/read/22225844/the-origin-and-ecological-significance-of-multiple-branches-for-histidine-utilization-in-pseudomonas-aeruginosa-pao1
#1
Monica L Gerth, Matteo P Ferla, Paul B Rainey
Pseudomonas proliferate in a wide spectrum of harsh and variable environments. In many of these environments, amino acids, such as histidine, are a valuable source of carbon, nitrogen and energy. Here, we demonstrate that the histidine uptake and utilization (hut) pathway of Pseudomonas aeruginosa PAO1 contains two branches from the intermediate formiminoglutamate to the product glutamate. Genetic analysis revealed that the four-step route is dispensable as long as the five-step route is present (and vice versa)...
August 2012: Environmental Microbiology
https://www.readbyqxmd.com/read/19741600/repeated-vaginal-administration-of-trimeric-hiv-1-clade-c-gp140-induces-serum-and-mucosal-antibody-responses
#2
M P Cranage, C A Fraser, Z Stevens, J Huting, M Chang, S A Jeffs, M S Seaman, A Cope, T Cole, R J Shattock
Vaccine-mediated prevention of primary infection with human immunodeficiency virus (HIV) may require the sustained production of antibody at mucosal portals of entry. Here, we describe a novel approach of repeated mucosal immunization by delivering an HIV-1 envelope glycoprotein (gp) in a gel formulated for intravaginal delivery. Rabbits were immunized over one to three 19-day cycles of intravaginal dosing with soluble recombinant trimeric HIV-1 clade C gp140 administered in Carbopol gel. The formulation was well tolerated...
January 2010: Mucosal Immunology
https://www.readbyqxmd.com/read/18774242/identification-of-iron-regulated-genes-in-the-fish-pathogen-aeromonas-salmonicida-subsp-salmonicida-genetic-diversity-and-evidence-of-conserved-iron-uptake-systems
#3
Mohsen Najimi, Manuel L Lemos, Carlos R Osorio
In this study we applied the Fur titration assay (FURTA) to isolate iron regulated genes in Aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in fish. We have identified genes for siderophore biosynthesis and for ferri-siderophore transport, some of which have been previously described in this species. A gene for a hitherto uncharacterized functional hemin receptor HutE was identified, but its inactivation did not affect significantly the use of hemin as sole iron source, suggesting that redundant genes encoding hemin receptors exist in the A...
February 2, 2009: Veterinary Microbiology
https://www.readbyqxmd.com/read/18205296/dynamic-combinatorial-mass-spectrometry-leads-to-metallo-beta-lactamase-inhibitors
#4
Benoît M R Liénard, Rebekka Hüting, Patricia Lassaux, Moreno Galleni, Jean-Marie Frère, Christopher J Schofield
The use of protein ESI mass spectrometry under non-denaturing conditions to analyze a dynamic combinatorial library of thiols/disulfides with the BcII metallo-beta-lactamase enabled the rapid identification of an inhibitor with a K(i) of < 1 microM. The study exemplifies the utility of protein-MS for screening dynamic mixtures of potential enzyme-inhibitors.
February 14, 2008: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/17493165/factors-affecting-the-success-rate-of-treatment-of-recumbent-dairy-cows-suffering-from-hypocalcaemia
#5
C-C Gelfert, I Alpers, M Dallmeyer, M Decker, A Hüting, S Lesch, W Baumgartner, R Staufenbiel
We aimed to investigate the ratio of accompanying diseases in cows suffering from clinical hypocalcaemia and their influence on cure rate. In five veterinary practices in different regions of Germany, all recumbent cows around parturition were included in the study for a period of 1 year. After recording the case history a clinical examination was done and a serum sample was taken to measure the concentrations of calcium and phosphorus magnesium, beta-hydroxybutyrate, total bilirubin, cholesterol, urea, and the activities of ASAT, CK, and GLDH...
May 2007: Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine
https://www.readbyqxmd.com/read/17080502/colloidal-gold-based-immunochromatographic-assay-for-detection-of-diethylstilbestrol-residues
#6
Xu Chuanlai, Wang Huting, Peng Chifang, Jin Zhengyu, Liu Liqiang
One-step membrane-based competitive colloidal gold-based immunoassays in immunochromatographic formats for the rapid detection of diethylstilbestrol (DES) were developed. Nitro-cellulose membrane strip was separately coated with goat anti-rabbit IgG (control line) and DES hapten-ovalubumin conjugate (test line). Anti-DES polyclonal antibody labeled with colloidal gold particles was first incubated with DES. A positive reaction as a result of the remaining antibody-gold conjugate combining with antigen coated on the membrane was obvious by visual detection, with detection limits for immunochromatographic of 0...
December 2006: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/16138832/the-activation-function-1-domain-of-estrogen-receptor-alpha-in-uterine-stromal-cells-is-required-for-mouse-but-not-human-uterine-epithelial-response-to-estrogen
#7
Takeshi Kurita, Roanna Medina, Alex B Schabel, Peter Young, Patricia Gama, Trilok V Parekh, Joel Brody, Gerald R Cunha, Kevin G Osteen, Kaylon L Bruner-Tran, Leslie I Gold
The activation function-1 (AF-1) domain of the estrogen receptor alpha (ERalpha) in stromal cells has been shown to be required for epithelial responses to estrogen in the mouse uterus. To investigate the role of the stroma in estrogenic responses of human uterine epithelium (hUtE), human/mouse chimeric uteri composed of human epithelium and mouse stroma were prepared as tissue recombinants (TR) that were grown in vivo under the renal capsule of female nude mouse hosts. In association with mouse uterine stroma (mUtS), hUtE formed normal glands surrounded by mouse endometrial stroma and the human epithelium influenced the differentiation of stroma into myometrium, such that a histologically normal appearing uterine tissue was formed...
July 2005: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/13613214/-electron-microscopic-patterns-of-starch-products-in-milk-preparations-for-infants
#8
E HUTE
No abstract text is available yet for this article.
September 1958: Monatsschrift Für Kinderheilkunde
https://www.readbyqxmd.com/read/10969629/diastolic-left-ventricular-function-after-renal-transplantation-in-patients-with-normal-and-hypertrophied-myocardium
#9
COMPARATIVE STUDY
J Hüting
While diastolic left ventricular (LV) dysfunction is frequent and associated with cardiovascular complications in end-stage renal disease treated with dialysis, controversial information exists on diastolic LV function after renal transplantation. Therefore, Doppler echocardiographic parameters of LV diastolic filling were analyzed in 17 transplanted patients with normal LV mass (< 150 g/m2; mean: 128 +/- 17 g/m2) and 24 transplanted patients with LV hypertrophy (> 150 g/m2; mean: 197 +/- 36 g/m2) and compared with 28 normal controls without and 11 controls with LV hypertrophy...
November 1992: Clinical Cardiology
https://www.readbyqxmd.com/read/8502338/predictive-value-of-mitral-and-aortic-valve-sclerosis-for-survival-in-end-stage-renal-disease-on-continuous-ambulatory-peritoneal-dialysis
#10
J Hüting
To determine whether mitral valve or anular sclerosis or calcification (MC) is associated with reduced survival in patients with end-stage renal disease on continuous ambulatory peritoneal dialysis (CAPD), 53 CAPD patients were followed with echocardiography and Doppler echocardiography over 35 months. Both nonsurvivors and survivors with MC had higher systolic blood pressure before CAPD and calcium-phosphorus products during CAPD treatment than patients without MC (p < 0.05). Serum calcium and phosphorus concentrations, alkaline phosphatase and parathyroid hormone activities were higher in nonsurvivors and survivors with than without MC (p > 0...
1993: Nephron
https://www.readbyqxmd.com/read/8448922/course-of-left-ventricular-diastolic-dysfunction-in-end-stage-renal-disease-on-long-term-continuous-ambulatory-peritoneal-dialysis
#11
J Hüting, M A Alpert
To determine factors contributing to abnormal left ventricular (LV) diastolic filling in patients on long-term continuous ambulatory peritoneal dialysis (CAPD), 11 (age: 55 +/- 13 years; CAPD duration: 33 +/- 32 months) out of 42 originally studied CAPD patients were followed over 35 months with echocardiography and pulsed Doppler echocardiography. LV dimensions and systolic function remained normal. Doppler parameters indicated diastolic LV dysfunction in 9/11 patients in the initial study. Despite a significant increase in LV mass (234 +/- 54 vs...
February 1993: Clinical Nephrology
https://www.readbyqxmd.com/read/8351942/-cardiac-characteristics-of-patients-with-terminal-renal-failure-after-kidney-transplantation
#12
J Hüting
Left ventricular (LV) hypertrophy and dysfunction are risk factors for high mortality from cardiovascular causes in patients with end-stage renal disease. To determine the frequency of these findings after kidney transplantation, 45 patients (11 female, 34 male; age: 47.2 +/- 12 years) after transplantation (57 +/- 37 months) with (n = 21) and without (n = 24) a patent arteriovenous fistula were analyzed with echocardiography. A high prevalence of systolic (> 140 mm Hg: 40%) and diastolic (> 90 mm Hg: 18%) hypertension was observed despite the use of 1...
June 1993: Zeitschrift Für Kardiologie
https://www.readbyqxmd.com/read/8306733/mitral-valve-calcification-as-an-index-of-left-ventricular-dysfunction-in-patients-with-end-stage-renal-disease-on-peritoneal-dialysis
#13
J Hüting
To determine whether mitral valve or anulus calcification (MC) in patients with end-stage renal disease is associated with abnormalities of left ventricular (LV) structure and function, cardiac characteristics of 55 patients undergoing continuous ambulatory peritoneal dialysis (CAPD) with (n = 26; age: 59 +/- 10 years) vs without (n = 29; age: 58 +/- 12 years) MC were analyzed using echocardiography and Doppler echocardiography. Sclerosis of the mitral valve anulus was detected in 18 (7 women, 11 men; age: 58 +/- 10 years) patients, sclerosis of mitral valve leaflets in 24 (13 women, 9 men; age: 59 +/- 10 years) patients...
February 1994: Chest
https://www.readbyqxmd.com/read/8174358/noninvasive-assessment-of-left-ventricular-structure-and-function-in-patients-with-end-stage-renal-disease
#14
REVIEW
M A Alpert, V Wizemann, J Hüting, C V Massey
No abstract text is available yet for this article.
1994: Contributions to Nephrology
https://www.readbyqxmd.com/read/8174355/importance-of-arterial-hypertension-for-left-ventricular-hypertrophy-in-patients-after-kidney-transplantation
#15
J Hüting, C Naumann-Hüting, V Wizemann
No abstract text is available yet for this article.
1994: Contributions to Nephrology
https://www.readbyqxmd.com/read/7734562/continuous-ambulatory-peritoneal-dialysis-and-the-heart
#16
REVIEW
M A Alpert, J Hüting, Z J Twardowski, R Khanna, K D Nolph
OBJECTIVE: To review clinical research pertaining to continuous ambulatory peritoneal dialysis (CAPD) and the heart. DATA SOURCES: A Medline computer search was employed to identify appropriate references from 1970 - 1994. Indexing terms were: continuous ambulatory peritoneal dialysis, hemodialysis, heart or cardiac, left ventricle, coronary artery disease, and survival. English and non-English language abstracts were scrutinized. STUDY SELECTION: Forty-six studies were reviewed and utilized...
1995: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
https://www.readbyqxmd.com/read/5377765/unconventional-nucleotide-analogues-i-n9-purinyl-alpha-amino-acids
#17
A J Nollet, C M Huting, U K Pandit
No abstract text is available yet for this article.
December 1969: Tetrahedron
https://www.readbyqxmd.com/read/3395168/cardiac-glycoside-receptors-in-clinical-and-experimental-states-of-chronic-renal-failure
#18
F Lübbecke, J Hüting, V Steudle, W Kramer, G Schütterle, V Wizemann
To investigate the mechanisms leading to decreased cardiac glycoside sensitivity in uremia, digitalis receptors were characterized by [3H]-ouabain binding to mononuclear leucocytes of chronically hemodialyzed patients and to cerebral cortex membranes of partially nephrectomized rats. There were no statistically significant differences in receptor density and affinity between uremic patients and healthy control persons and between partially nephrectomized and sham operated rats. [3H]-Ouabain bound with high affinity to intact mononuclear leucocytes and to cerebral cortex membranes...
March 1988: Archives Internationales de Pharmacodynamie et de Thérapie
https://www.readbyqxmd.com/read/3037728/an-in-vitro-model-for-studying-the-toxicity-of-2-3-7-8-tetrachlorodibenzo-p-dioxin-to-human-thymus
#19
J C Cook, K M Dold, W F Greenlee
A coculture system of human thymic epithelial (HuTE) cells and thymocytes (T lymphocyte precursors) has been established and characterized as an in vitro model for assessing the potential toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to human thymus. HuTE cells in culture were responsive to TCDD as judged by induction of the cytochrome P1-450 monooxygenase activities, 7-ethoxycoumarin O-deethylase (ECOD) and 7-ethoxyresorufin O-deethylase (EROD). Measurement of the responsiveness of thymocytes cocultured on TCDD-pretreated HuTE monolayers to the mitogens concanavalin A (Con A) and phytohemagglutinin (PHA) indicated that TCDD can act directly on HuTE cells to suppress thymocyte maturation (at a concentration of 10 nM, TCDD produced a 25 to 50% inhibition of thymocyte responsiveness to Con A and PHA)...
June 30, 1987: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/2970599/analysis-of-left-ventricular-changes-associated-with-chronic-hemodialysis-a-noninvasive-follow-up-study
#20
J Hüting, W Kramer, G Schütterle, V Wizemann
To assess the reasons for the frequent cardiovascular complications in patients with end-stage renal disease (ESRD), 61 out of 131 normotensive ESRD patients originally examined (mean ESRD duration: 71 +/- 41 months) were followed over 2.5 years by echo-, electro- and mechanocardiography. Clinical and biochemical parameters were comparable. The prevalence of pericardial effusion (3%), pericardial thickening (14%), aortic valve sclerosis (14%) and mitral valve anulus sclerosis (12%) was unchanged. The interventricular septum diameter (14...
1988: Nephron
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