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https://www.readbyqxmd.com/read/28918155/the-binding-of-orally-dosed-hydrophobic-active-pharmaceutical-ingredients-to-casein-micelles-in-milk
#1
M Cheema, A N Hristov, F M Harte
Casein proteins (αS1-, αS2-, β- and κ-casein) account for 80% of the total protein content in bovine milk and form casein micelles ( D¯11 = 130 nm, approximately 10(15) micelles/mL). The affinity of native casein micelles with the 3 hydrophobic active pharmaceutical ingredients (API), meloxicam [351.4 g/mol; log P = 3.43; acid dissociation constant (pKa) = 4.08], flunixin (296.2 g/mol; log P = 4.1; pKa = 5.82), and thiabendazole (201.2 g/mol; log P = 2.92; pKa = 4.64), was evaluated in bovine milk collected from dosed Holstein cows...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28917922/the-effects-of-lotus-root-amylopectin-on-the-formation-of-whey-protein-isolate-gels
#2
Kang Liu, Qiang-Ming Li, Li-Hua Pan, Xin-Ping Qian, Hai-Lin Zhang, Xue-Qiang Zha, Jian-Ping Luo
The effects of lotus root amylopectin (LRA) on the formation of whey protein isolate (WPI) gels were investigated at the concentration range from 0 to 1.0% (w/v) by determining the texteral, thermal and rheological properties. Results exhibited these properties of WPI gels could be significantly enhanced by the addition of LRA in a concentration-dependent manner. Compared the gel with free of starch, the gel strength, water holding capacity and thermal transition temperature of WPI gel containing 1% (w/v) amylopectin were enhanced by 12...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28916571/effect-of-soy-and-soy-isoflavones-on-obesity-related-anthropometric-measures-a-systematic-review-and-meta-analysis-of-randomized-controlled-clinical-trials
#3
REVIEW
Masoumeh Akhlaghi, Morteza Zare, Fatemeh Nouripour
Soy may be a suitable food for anti-obesity efforts because of its high protein and isoflavone content. We conducted this meta-analysis to evaluate potential effects of soy and soy isoflavones on weight, waist circumference, and fat mass. PubMed, MEDLINE, Scopus, EMBASE, and Cochrane databases were searched. Twenty-four trials with soy and 17 trials with isoflavones passed the eligibility stage. According to the results, soy showed no overall statistically significant effect on weight, waist circumference, or fat mass, but a significant increasing effect on weight was observed in some circumstances: for instance, in obese subjects [mean difference (MD): 0...
September 2017: Advances in Nutrition
https://www.readbyqxmd.com/read/28910233/effects-of-supplementation-with-beef-or-whey-protein-versus-carbohydrate-in-master-triathletes
#4
Fernando Naclerio, Marco Seijo, Eneko Larumbe-Zabala, Nadia Ashrafi, Tatiana Christides, Bettina Karsten, Birthe V Nielsen
OBJECTIVE: The present study compares the effect of ingesting hydrolyzed beef protein, whey protein, and carbohydrate on performance, body composition (via plethysmography), muscular thickness, and blood indices of health, including ferritin concentrations, following a 10-week intervention program. METHODS: After being randomly assigned to one of the following groups-beef, whey, or carbohydrate-24 master-age (35-60 years old) male triathletes (n = 8 per treatment) ingested 20 g of supplement mixed with plain water once a day (immediately after training or before breakfast)...
September 14, 2017: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/28906440/native-and-heated-hydrolysates-of-milk-proteins-and-their-capacity-to-inhibit-lipid-peroxidation-in-the-zebrafish-larvae-model
#5
Wilman Carrillo, Xavier Guzmán, Edgar Vilcacundo
Casein and whey proteins with and without heat treatment were obtained of whole milk and four commercial milks in Ecuador, and were hydrolyzed. Then, their capacity to inhibit the lipid peroxidation using the TBARS method was evaluated at concentrations of 0.02, 0.04, 0.2, and, 0.4 mg/mL. Native and heated hydrolysates of milk proteins present high inhibitions of lipid peroxidation with a dose dependent effect both in vivo and in vitro tests. Casein and whey proteins obtained from whole milk were the ones with the highest anti-oxidant activity in vitro and in vivo test...
September 14, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28899371/simultaneous-detection-of-%C3%AE-lactoalbumin-%C3%AE-lactoglobulin-and-lactoferrin-in-milk-by-visualized-microarray
#6
Zhoumin Li, Fang Wen, Zhonghui Li, Nan Zheng, Jindou Jiang, Danke Xu
BACKGROUND: α-Lactalbumin (a-LA), β-lactoglobulin (β-LG) and lactoferrin (LF) are of high nutritional value which have made ingredients of choice in the formulation of modern foods and beverages. There remains an urgent need to develop novel biosensing methods for quantification featuring reduced cost, improved sensitivity, selectivity and more rapid response, especially for simultaneous detection of multiple whey proteins. RESULTS: A novel visualized microarray method was developed for the determination of a-LA, β-LG and LF in milk samples without the need for complex or time-consuming pre-treatment steps...
September 12, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28898422/dynamic-gastric-digestion-of-a-commercial-whey-protein-concentrate
#7
Beatriz Miralles, Roberto Del Barrio, Carolina Cueva, Isidra Recio, Lourdes Amigo
BACKGROUND: A dynamic gastrointestinal simulator, simgi(®) , has been applied to assess the gastric digestion of a whey protein concentrate. Samples collected from the outlet of the stomach have been compared to those resulting from the static digestion protocol INFOGEST developed on the basis of physiologically inferred conditions. RESULTS: Progress of digestion was followed by SDS-PAGE and LC-MS/MS. By SDS-PAGE, serum albumin and α-lactalbumin were no longer detectable at 30 and 60 min, respectively...
September 12, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28894632/dietary-incorporation-of-whey-protein-isolate-and-galactooligosaccharides-exhibits-improvement-in-glucose-homeostasis-and-insulin-resistance-in-high-fat-diet-fed-mice
#8
Praveen Kumar Kavadi, Ramesh Pothuraju, Jayasimha Chagalamarri, Gaurav Bhakri, Aswani Mallepogu, Raj Kumar Sharma
BACKGROUND: This study was planned to investigate the effectiveness of the whey protein isolate (WPI) of high purity and a galactooligosaccharides (GOS) preparation on glucose homeostasis and insulin resistance in high fat diet (HFD) (45.47% energy from fat) fed conditions in C57BL/6J mice. METHODS: Fasting blood glucose level, serum insulin, and glucagon-like peptide-1 (enzyme-linked immunosorbent assay) were measured; also, homeostasis model assessment of insulin resistance (HOMA-IR) was determined in different treatment groups...
July 2017: Journal of Intercultural Ethnopharmacology
https://www.readbyqxmd.com/read/28894506/a-cystine-rich-whey-supplement-immunocal%C3%A2-provides-neuroprotection-from-diverse-oxidative-stress-inducing-agents-in-vitro-by-preserving-cellular-glutathione
#9
Aimee N Winter, Erika K Ross, Vamsi Daliparthi, Whitney A Sumner, Danielle M Kirchhof, Evan Manning, Heather M Wilkins, Daniel A Linseman
Oxidative stress is a principal mechanism underlying the pathophysiology of neurodegeneration. Therefore, nutritional enhancement of endogenous antioxidant defenses may represent a viable treatment option. We investigated the neuroprotective properties of a unique whey protein supplement (Immunocal®) that provides an essential precursor (cystine) for synthesis of the endogenous antioxidant, glutathione (GSH). Primary cultures of rat cerebellar granule neurons (CGNs), NSC34 motor neuronal cells, or HT22 hippocampal cells were preincubated in medium containing Immunocal and then subsequently treated with agents known to induce oxidative stress...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28888462/control-of-protein-digestion-under-simulated-gastrointestinal-conditions-using-biopolymer-microgels
#10
Zipei Zhang, Ruojie Zhang, David Julian McClements
Controlling the rate and extent of protein digestion within different regions of the human gastrointestinal tract (GIT) is important for regulating hormonal responses (such as hunger, satiety, and satiation) and immune responses (such as allergenicity). In this study, hydrogel beads (biopolymer microgels) were fabricated by injecting a solution of anionic alginate molecules into a solution of cationic calcium ions using a vibrating extrusion device. A model food protein (whey protein isolate, WPI) was mixed with the alginate solution prior to bead formation...
October 2017: Food Research International
https://www.readbyqxmd.com/read/28888456/dry-heating-of-whey-proteins
#11
REVIEW
Elise Schong, Marie-Hélène Famelart
Whey protein products are of widespread use as food ingredients because of their high nutritional, biological and functional properties. Whey proteins are important structural components in many foods as used in their native form, for example for their heat-induced gelation abilities. Furthermore, they also offer reliable functionalities when modified by heating processes as denatured or aggregated proteins. Heat treatment of whey proteins in a liquid state has received much attention in recent years. While dry heating of whey proteins, say heating whey proteins in the dry state, is frequently cited in the literature as a potential and efficient means to improve the functional properties of proteins, it has received very little attention...
October 2017: Food Research International
https://www.readbyqxmd.com/read/28888438/impact-of-legume-protein-type-and-location-on-lipid-oxidation-in-fish-oil-in-water-emulsions-lentil-pea-and-faba-bean-proteins
#12
Cansu Ekin Gumus, Eric Andrew Decker, David Julian McClements
Emulsion-based delivery systems are being developed to incorporate ω-3 fatty acids into functional foods and beverages. There is interest in formulating these delivery systems from more sustainable and label-friendly ingredients. The aim of this study was therefore to examine the impact of plant-protein emulsifiers on the oxidative stability of 1wt% fish oil-in-water emulsions. Fish oil emulsions stabilized by three types of legume protein (lentil, pea, and faba bean) were produced using a high-pressure microfluidizer...
October 2017: Food Research International
https://www.readbyqxmd.com/read/28878590/enzymatically-modified-isoquercitrin-supplementation-intensifies-plantaris-muscle-fiber-hypertrophy-in-functionally-overloaded-mice
#13
Akiko Kohara, Masanao Machida, Yuko Setoguchi, Ryouichi Ito, Masanori Sugitani, Hiroko Maruki-Uchida, Hiroyuki Inagaki, Tatsuhiko Ito, Naomi Omi, Tohru Takemasa
BACKGROUND: Enzymatically modified isoquercitrin (EMIQ) is produced from rutin using enzymatic hydrolysis followed by treatment with glycosyltransferase in the presence of dextrin to add glucose residues. EMIQ is absorbed in the same way as quercetin, a powerful antioxidant reported to prevent disused muscle atrophy by targeting mitochondria and to have ergogenic effects. The present study investigated the effect of EMIQ on skeletal muscle hypertrophy induced by functional overload. METHODS: In Study 1, 6-week-old ICR male mice were divided into 4 groups: sham-operated control, sham-operated EMIQ, overload-operated control, and overload-operated EMIQ groups...
2017: Journal of the International Society of Sports Nutrition
https://www.readbyqxmd.com/read/28877767/feeding-broilers-with-dry-whey-powder-and-whey-protein-concentrate-affected-productive-performance-ileal-digestibility-of-nutrients-and-cecal-microbiota-community
#14
C Pineda-Quiroga, A Camarinha-Silva, D Borda-Molina, R Atxaerandio, R Ruiz, A García-Rodríguez
Dietary interventions are a common practice in the poultry industry to promote optimal performance and health of animals. Here, we aim at assessing the influence of supplementing broiler diets with dry whey powder (DWP) and whey protein concentrate (WPC) on nutrient coefficient of apparent ileal digestibility (CAID) and productive performance. Cecal microbiota composition was also determined using Illumina amplicon sequencing. Dietary treatments were control diet (no supplementation of DWP or WPC), 60-DWP (60 g/kg of DWP), and 80-WPC (80 g/kg of WPC)...
September 7, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28876921/binding-of-gallic-acid-and-epigallocatechin-gallate-to-heat-unfolded-whey-proteins-at-neutral-ph-alters-radical-scavenging-activity-of-in-vitro-protein-digests
#15
Yanyun Cao, Youling L Xiong
Preheated (80 °C for 9 min) whey protein isolate (HWPI) was reacted with 20, 120, and 240 μmol/g (protein basis) gallic acid (GA) or epigallocatechin gallate (EGCG) at neutral pH and 25 °C. Isothermal titration calorimetry and fluorometry showed similar trend that GA binding to HWPI was moderate but weaker than EGCG binding. However, the shift of maximal fluorescence emission wavelength in opposite directions in response to GA (blue) and EGCG (red) suggests discrepant binding patterns. Electrophoresis results showed that EGCG induced formation of HWPI complexes while GA only had a marginal effect...
September 6, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28876131/effects-of-ultrasound-treatment-on-physiochemical-properties-and-antimicrobial-activities-of-whey-protein-totarol-nanoparticles
#16
Shuang Ma, Ce Shi, Cuina Wang, Mingruo Guo
Totarol is a natural antimicrobial compound extracted from the heartwood of Podocarpus totara, a conifer native to New Zealand. The effects of whey protein-totarol nanoparticles treated with ultrasound on the physiochemical properties and the growth of Staphylococcus aureus were investigated. The particle size of whey protein-totarol nanoparticles was reduced by ultrasound treatment from 31.24 ± 5.31 to 24.20 ± 4.02 nm, and the size distribution was also narrowed by the treatment. Viscosity and modulus data indicated that the flow behaviors of whey protein-totarol nanoparticles seemed to be Newtonian and exerted a typical viscoelastic fluid at protein content of 15% (w/v)...
September 6, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28875201/ginseng-panax-ginseng-meyer-oligopeptides-regulate-innate-and-adaptive-immune-responses-in-mice-via-increased-macrophage-phagocytosis-capacity-nk-cell-activity-and-th-cells-secretion
#17
Li-Xia He, Jin-Wei Ren, Rui Liu, Qi-He Chen, Jian Zhao, Xin Wu, Zhao-Feng Zhang, Jun-Bo Wang, Giuseppe Pettinato, Yong Li
Traditionally used as a restorative medicine, ginseng (Panax ginseng Meyer) has been the most widely used and acclaimed herb in Chinese communities for thousands of years. To investigate the immune-modulating activity of ginseng oligopeptides (GOP), 420 healthy female BALB/c mice were intragastrically administered distilled water (control), whey protein (0.15 g per kg body weight (BW)), and GOP 0.0375, 0.075, 0.15, 0.3 and 0.6 g per kg BW for 30 days. Blood samples from mice were collected from the ophthalmic venous plexus and then sacrificed by cervical dislocation...
September 6, 2017: Food & Function
https://www.readbyqxmd.com/read/28873736/formation-and-characterization-of-supramolecular-structures-of-%C3%AE-lactoglobulin-and-lactoferrin-proteins
#18
Camila Santiago Saraiva, Jane Sélia Dos Reis Coimbra, Alvaro Vianna Novaes de Carvalho Teixeira, Eduardo Basílio de Oliveira, Reinaldo Francisco Teófílo, Angélica Ribeiro da Costa, Éverton de Almeida Alves Barbosa
Combination of β-lactoglobulin (β-Lg) and lactoferrin (Lf), biomacromolecules derived from bovine whey, was used in the formation of supramolecular structures by thermal gelation technique to adjust the pH. Furthermore, the influence of the molar ratio, temperature, pH, and heating time in the formation of supramolecular structures were also studied. The characterization of the protein supramolecular structures was performed using dynamic light scattering, zeta potential measurements, molecular spectrofluorimetry, and circular dichroism spectroscopy...
October 2017: Food Research International
https://www.readbyqxmd.com/read/28873631/enhancing-physicochemical-properties-of-emulsions-by-heteroaggregation-of-oppositely-charged-lactoferrin-coated-lutein-droplets-and-whey-protein-isolate-coated-dha-droplets
#19
Xin Li, Xu Wang, Duoxia Xu, Yanping Cao, Shaojia Wang, Bei Wang, Baoguo Sun, Fang Yuan, Yanxiang Gao
The formation and physicochemical stability of mixed functional components (lutein & DHA) emulsions through heteroaggregation were studied. It was formed by controlled heteroaggregation of oppositely charged lutein and DHA droplets coated by cationic lactoferrin (LF) and anionic whey protein isolate (WPI), respectively. Heteroaggregation was induced by mixing the oppositely charged LF-lutein and WPI-DHA emulsions together at pH 6.0. Droplet size, zeta-potential, transmission-physical stability, microrheological behavior and microstructure of the heteroaggregates formed were measured as a function of LF-lutein to WPI-DHA droplet ratio...
January 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28873595/protein-degradation-and-peptide-release-from-milk-proteins-in-human-jejunum-comparison-with-in-vitro-gastrointestinal-simulation
#20
J Sanchón, S Fernández-Tomé, B Miralles, B Hernández-Ledesma, D Tomé, C Gaudichon, I Recio
Human jejunal digests after oral ingestion of casein and whey protein were collected by a nasogastric tube and protein degradation and peptide release was compared with that found in the digests of the same substrates using a standardised protocol. No intact casein was detected in the jejunal nor in the in vitro samples taken during the intestinal phase, while β-lactoglobulin was found in one hour-jejunal samples in agreement with the in vitro digestion. In vivo and in vitro digests showed comparable peptide profiles and high number of common sequences...
January 15, 2018: Food Chemistry
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