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https://www.readbyqxmd.com/read/28214599/hctoll3-was-involved-in-anti-vibrio-defense-in-freshwater-pearl-mussel-hyriopsis-cumingii
#1
Hong-Wei Zhang, Ying Huang, Xin Man, Yue Wang, Kai-Min Hui, Shao-Wu Yin, Xiao-Wen Zhang
Toll-like receptors (TLRs) play an important role in the activation of innate immune response but their functions in bivalves remain largely unknown. In this study, we identified a TLR from the freshwater pearl mussel Hyriopsis cumingii (HcToll3) and investigated its functions in immunity. The full-length cDNA of HcToll3 is 3852 bp and includes an open reading frame (ORF) of 3228 bp that encodes a polypeptide of 1075 amino acids. The predicted HcToll3 protein shares similar structural characteristics with other known Toll family proteins...
February 15, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28194093/effects-of-an-amylopectin-and-chromium-complex-on-the-anabolic-response-to-a-suboptimal-dose-of-whey-protein
#2
T N Ziegenfuss, H L Lopez, A Kedia, S M Habowski, J E Sandrock, B Raub, C M Kerksick, A A Ferrando
BACKGROUND: Previous research has demonstrated the permissive effect of insulin on muscle protein kinetics, and the enhanced insulin sensitizing effect of chromium. In the presence of adequate whole protein and/or essential amino acids (EAA), insulin has a stimulatory effect on muscle protein synthesis, whereas in conditions of lower blood EAA concentrations, insulin has an inhibitory effect on protein breakdown. In this study, we determined the effect of an amylopectin/chromium (ACr) complex on changes in plasma concentrations of EAA, insulin, glucose, and the fractional rate of muscle protein synthesis (FSR)...
2017: Journal of the International Society of Sports Nutrition
https://www.readbyqxmd.com/read/28193434/the-effect-of-non-standard-heat-treatment-of-sheep-s-milk-on-physico-chemical-properties-sensory-characteristics-and-the-bacterial-viability-of-classical-and-probiotic-yogurt
#3
Šimun Zamberlin, Dubravka Samaržija
Classical and probiotic set yogurt were made using non-standard heat treatment of sheep's milk at 60°C/5min. Physico-chemical properties, sensory characteristics, and the viability of bacteria that originated from cultures in classical and probiotic yogurt were analysed during 21days of storage at 4°C. For the production of yogurt, a standard yogurt culture and a probiotic strain Lactobacillus rhamnosus GG were used. At the end of storage time of the classical and probiotic yogurt the totals of non-denatured whey proteins were 92...
June 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28191637/mammary-alveolar-epithelial-cells-convert-to-brown-adipocytes-in-post-lactating-mice
#4
Antonio Giordano, Jessica Perugini, David M Kristensen, Loris Sartini, Andrea Frontini, Shingo Kajimura, Karsten Kristiansen, Saverio Cinti
During pregnancy and lactation, subcutaneous white adipocytes in the mouse mammary gland transdifferentiate reversibly to milk-secreting epithelial cells. In this study, we demonstrate by transmission electron microscopy that in the post-lactating mammary gland interscapular multilocular adipocytes found close to the mammary alveoli contain milk protein granules. Use of the Cre-loxP recombination system allowed showing that the involuting mammary gland of whey acidic protein-Cre/R26R mice, whose secretory alveolar cells express the lacZ gene during pregnancy, contains some X-Gal-stained and uncoupling protein 1-positive interscapular multilocular adipocytes...
February 12, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28161624/mixotrophic-cultivation-of-chlorella-for-local-protein-production-using-agro-food-by-products
#5
Silvia Salati, Giuliana D'Imporzano, Barbara Menin, Davide Veronesi, Barbara Scaglia, Pamela Abbruscato, Paola Mariani, Fabrizio Adani
A local strain of Chlorella vulgaris was cultivated by using cheese whey (CW), white wine lees (WL) and glycerol (Gly), coming from local agro-industrial activities, as C sources (2.2gCL(-1)) to support algae production under mixotrophic conditions in Lombardy. In continuous mode, Chlorella increased biomass production compared with autotrophic conditions by 1.5-2 times, with the best results obtained for the CW substrate, i.e. 0.52gL(-1)d(-1) of algal biomass vs. 0.24gL(-1)d(-1) of algal biomass for autotrophic conditions, and protein content for both conditions adopted close to 500gkg(-1) DM...
January 16, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28159254/identification-and-quantification-of-ace-inhibiting-peptides-in-enzymatic-hydrolysates-of-plant-proteins
#6
Steffi Rudolph, Diana Lunow, Susanne Kaiser, Thomas Henle
Enzymatic hydrolysis of proteins from rice, soy, pea and wheat, with both chymotrypsin and thermolysin, resulted in hydrolysates, which are efficient inhibitors of the angiotensin-converting enzyme (ACE). IC50 values of the hydrolysates were between 27 and 39mg/l, which is comparable to enzymatically hydrolysed whey protein. A significant increase of the ACE-inhibiting effect was observed following butanol extraction due to accumulation of hydrophobic peptides (IC50 between 12 and 21mg/l). Based on the identification and quantification of individual tryptophan-, tyrosine- and phenylalanine-containing dipeptides, 50-80% of the total ACE-inhibiting potential of butanol extracts from plant protein hydrolysates could be explained...
June 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28155071/sequential-application-of-waste-whey-as-a-medium-component-for-kluyveromyces-lactis-cultivation-and-a-co-feeder-for-lipase-immobilization-by-clea-method
#7
Aušra Veteikytė, Rimantas Šiekštelė, Bronius Tvaska, Inga Matijošytė
Currently, much attention is paid to technologies which can be drivers of the circular economy across different sectors, in particular, to develop technologies for utilization or reusability of biocompatible materials from industrial waste. One of such is the milk whey, which is a cheap biobased raw material, the disposal of which is a major problem for the dairy industry. Our proposed and investigated technology is based on a continuous exploitation of the whey combining microbiology and biotechnology. Primarily, whey was used as a nutrition source for the cultivation of Kluyveromyces lactis with the aim to produce the targeted biocatalyst-lipase...
February 2, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28153503/intake-of-tryptophan-enriched-whey-protein-acutely-enhances-recall-of-positive-loaded-words-in-patients-with-multiple-sclerosis
#8
Cindy K Lieben, Arjan Blokland, Nicolaas E Deutz, Willemijn Jansen, Gang Han, Raymond M Hupperts
BACKGROUND & AIMS: Multiple sclerosis (MS) has physiological and/or immunological characteristics that diminish serotonin metabolism, a neurotransmitter associated with affective and cognitive functions. The aim was examine the acute and dose-dependent effects of a dietary tryptophan (TRP) enrichment on affective and cognitive functions in MS patients. We hypothesized that increased dietary availability of the amino acid TRP enhances serotonin concentrations and improves neuropsychological functions...
January 16, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28142150/camel-whey-protein-protects-b-and-t-cells-from-apoptosis-by-suppressing-activating-transcription-factor-3-atf-3-mediated-oxidative-stress-and-enhancing-phosphorylation-of-akt-and-i%C3%AE%C2%BAb-%C3%AE-in-type-i-diabetic-mice
#9
Gamal Badr, Leila H Sayed, Hossam El-Din M Omar, Ali M Abd El-Rahim, Emad A Ahmed, Mohamed H Mahmoud
BACKGROUND: Diabetes mellitus (DM) is associated with severe immune system complications. Camel whey protein (CWP) decreases free radicals (ROS) and modulates immune functions, but its effect on DM-impaired immune systems has not been studied. We investigated the impact of CWP on the immune system in a Type 1 diabetes mouse model. METHODS: Three experimental groups were used: (1) non-diabetic control; (2) diabetic; and (3) CWP-treated diabetic mice. RESULTS: Induction of diabetes by streptozotocin was associated with reduction of body weight and insulin level, increase in glucose level and pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α), and reduction in IL-2 and IL-4 levels...
January 17, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28140770/explaining-the-texture-properties-of-whey-protein-isolate-starch-co-gels-from-fracture-structures
#10
Wei Fu, Takashi Nakamura
The effects of tapioca starch (TS) and potato starch (PS) on texture properties of whey protein isolate (WPI)/starch co-gels were investigated for fracture structures. We focused on two types of WPI network structures. In a fine-stranded structure at pH 6.8, the WPI/TS co-gel fractured similarly to the WPI single gel. The WPI/PS co-gel was broken at a lower strain and lower stress. In a random aggregation at pH 5.8, the WPI/TS co-gel reached a yielding point at a lower strain, whereas the WPI/PS co-gel fractured at a higher strain and higher stress...
January 31, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28140463/binding-of-caffeine-and-quinine-by-whey-protein-and-the-effect-on-bitterness
#11
Kelsey Tenney, John Hayes, Stephen Euston, Ryan Elias, John Coupland
Many drugs and phytochemicals are bitter, leading to noncompliance with prescriptions and avoidance of healthy foods and a need to suppress their taste. The goal of this study was to investigate the binding of bitterants (quinine and caffeine) by whey protein isolate (WPI) and the effect on perceived bitterness. Caffeine interacted minimally with WPI, while the proportion of unbound quinine decreased exponentially with protein concentration. Molecular modeling was used to show the energy of the quinine-Β-lactoglubulin interaction was an order of magnitude greater than the caffeine-Β-lactoglobulin interaction...
January 31, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28139153/effects-of-puddings-containing-whey-protein-and-polydextrose-on-subjective-feelings-of-appetite-and-short-term-energy-intake-in-healthy-adults
#12
Marcela Martinelli, Florencia Walz, Eva Goñi, Giovanna Passutti, Carlos Osella, Silvina R Drago
The aim of this study was to investigate the effects of puddings with whey protein (WP) and polidextrose (PX) on appetite feelings and energy intake. A single-blind randomized, crossover study was carried out in 25 healthy adults. Participants consumed two different puddings, including control or test pudding (12.9 g WP and 6 g PX). Two testing days were completed, including satiety questionnaires and ad libitum lunch. For all participants, energy intake at lunch was similar after consumption of control or test pudding...
January 31, 2017: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/28135405/the-potential-source-of-b-licheniformis-contamination-during-whey-protein-concentrate-80-manufacture
#13
Siti Norbaizura Md Zain, Rod Bennett, Steve Flint
The objective of this study was to determine the possible source of predominant Bacillus licheniformis contamination in a whey protein concentrate (WPC) 80 manufacturing plant. Traditionally, microbial contaminants of WPC were believed to grow on the membrane surfaces of the ultrafiltration plant as this represents the largest surface area in the plant. Changes from hot to cold ultrafiltration have reduced the growth potential for bacteria on the membrane surfaces. Our recent studies of WPCs have shown the predominant microflora B...
January 30, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28132725/sufficient-levels-of-25-hydroxyvitamin-d-and-protein-intake-required-to-increase-muscle-mass-in-sarcopenic-older-adults-the-provide-study
#14
Sjors Verlaan, Andrea B Maier, Jürgen M Bauer, Ivan Bautmans, Kirsten Brandt, Lorenzo M Donini, Marcello Maggio, Marion E T McMurdo, Tony Mets, Chris Seal, Sander L J Wijers, Cornel Sieber, Yves Boirie, Tommy Cederholm
BACKGROUND: Inadequate nutritional intake and altered response of aging muscles to anabolic stimuli from nutrients contribute to the development of sarcopenia. Nutritional interventions show inconsistent results in sarcopenic older adults, which might be influenced by their basal nutritional status. OBJECTIVE: To test if baseline serum 25-hydroxyvitamin D (25(OH)D) concentrations and dietary protein intake influenced changes in muscle mass and function in older adults who received nutritional intervention...
January 17, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28129627/camel-whey-protein-improves-oxidative-stress-and-histopathological-alterations-in-lymphoid-organs-through-bcl-xl-bax-expression-in-a-streptozotocin-induced-type-1-diabetic-mouse-model
#15
Leila H Sayed, Gamal Badr, Hossam M Omar, Ali M Abd El-Rahim, Mohamed H Mahmoud
Type I diabetes (T1D) is a characterized by the inflammation of pancreatic islets and destruction of β cells. Long and persistent uncontrolled diabetes tends to degenerate the immune system and increase the incidence of infections in diabetic individuals. Most serious diabetic complications are mediated by the free radicals, which damage multiple cellular components through direct effects of the cell cycle regulatory proteins. Camel whey protein (CWP) has antioxidant activity and decreases the effects of free radicals...
January 24, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28123065/flow-assisted-assembly-of-nanostructured-protein-microfibers
#16
Ayaka Kamada, Nitesh Mittal, L Daniel Söderberg, Tobias Ingverud, Wiebke Ohm, Stephan V Roth, Fredrik Lundell, Christofer Lendel
Some of the most remarkable materials in nature are made from proteins. The properties of these materials are closely connected to the hierarchical assembly of the protein building blocks. In this perspective, amyloid-like protein nanofibrils (PNFs) have emerged as a promising foundation for the synthesis of novel bio-based materials for a variety of applications. Whereas recent advances have revealed the molecular structure of PNFs, the mechanisms associated with fibril-fibril interactions and their assembly into macroscale structures remain largely unexplored...
February 7, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28122929/a-systematic-review-of-the-effects-of-plant-compared-with-animal-protein-sources-on-features-of-metabolic-syndrome
#17
REVIEW
Tristan Chalvon-Demersay, Dalila Azzout-Marniche, Judith Arfsten, Léonie Egli, Claire Gaudichon, Leonidas G Karagounis, Daniel Tomé
Dietary protein may play an important role in the prevention of metabolic dysfunctions. However, the way in which the protein source affects these dysfunctions has not been clearly established. The aim of the current systematic review was to compare the impact of plant- and animal-sourced dietary proteins on several features of metabolic syndrome in humans. The PubMed database was searched for both chronic and acute interventional studies, as well as observational studies, in healthy humans or those with metabolic dysfunctions, in which the impact of animal and plant protein intake was compared while using the following variables: cholesterolemia and triglyceridemia, blood pressure, glucose homeostasis, and body composition...
January 25, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28121797/the-effect-of-postexercise-carbohydrate-and-protein-ingestion-on-bone-metabolism
#18
Rebecca Townsend, Kirsty J Elliott-Sale, Kevin Currell, Jonathan Tang, William D Fraser, Craig Sale
PURPOSE: To investigate the effect of feeding carbohydrate and protein (CHO+PRO), immediately or 2 h after an exhaustive run, on the bone turnover response in endurance runners. METHODS: 10 men (age 28±5 y, height 1.74±0.05 m, body mass 69.7±6.3 kg) performed treadmill running at 75%VO2max, until exhaustion, on three occasions. Blood was collected before and immediately, 1, 2, 3, 4 and 24 h post-exercise, for measurement of β-CTX, P1NP, PTH, PO4, ACa and Ca...
January 24, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28121323/ingestion-of-soy-whey-blended-protein-augments-sports-performance-and-ameliorates-exercise-induced-fatigue-in-a-rat-exercise-model
#19
Guangxu Ren, Suqing Yi, Hongru Zhang, Jing Wang
This study sought to determine the effects of soy-whey blended protein supplementation on sports performance and related biochemical parameters after long-term training. After a week of adaptation, eighteen 6-week-old male Wistar rats were randomly assigned to 3 groups: the standard chow diet plus whey protein (Whey) group, the standard chow diet plus soy-whey blended protein (BP) group and the standard chow diet only (control) group. Each group included 6 rats for the seven-week experiment. Before the experiment, the baseline values of body weight, grasping force and time to exhaustion due to the loaded-swimming test were recorded for each group...
January 25, 2017: Food & Function
https://www.readbyqxmd.com/read/28109603/comparison-between-direct-and-indirect-methods-for-exploiting-fourier-transform-spectral-information-in-estimation-of-breeding-values-for-fine-composition-and-technological-properties-of-milk
#20
V Bonfatti, D Vicario, L Degano, A Lugo, P Carnier
The aim of this study was to compare the common method of exploiting infrared spectral data in animal breeding; that is, estimating the breeding values for the traits predicted by infrared spectroscopy, and an alternative approach based on the direct use of spectral information (direct prediction, DP) to predict the estimated breeding values (EBV). Traits were pH, milk coagulation properties, contents of the main casein and whey protein fractions, cheese yield measured by micro-cheese making, lactoferrin, Ca, and fat composition...
March 2017: Journal of Dairy Science
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