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Whey protein

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https://www.readbyqxmd.com/read/29768984/anti-cancer-study-and-whey-protein-complexation-of-new-lanthanum-iii-complex-with-the-aim-of-achieving-bioactive-anticancer-metal-based-drugs
#1
Somaye Shahraki, Fereshteh Shiri, Mostafa Heidari Majd, Somaye Dahmardeh
In this study a new lanthanum (III)-amino acid complex utilizing cysteine has been synthesized and characterized. The anticancer activities of the prepared La(III) complex against MCF-7 cell lines were studied. Results of MTT assay showed that at all three incubation times, the cytotoxic effect of prepared La(III) complex on MCF-7 breast cancer cell lines displays a time- and dose-dependent inhibitory effects. The interactions of the La(III) complex with two whey proteins (bovine serum albumin, BSA, and Bovine β-lactoglobulin, βLG) have been explored by using spectroscopic and molecular dicking methods...
May 17, 2018: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29764624/hydrolyzed-proteins-from-herring-and-salmon-rest-raw-material-contain-peptide-motifs-with-angiotensin-i-converting-enzyme-inhibitors-and-resulted-in-lower-urine-concentrations-of-protein-cystatin-c-and-glucose-when-fed-to-obese-zucker-fa-fa-rats
#2
Aslaug Drotningsvik, Daniela Maria Pampanin, Rasa Slizyte, Ana Carvajal, Ingmar Høgøy, Tore Remman, Oddrun Anita Gudbrandsen
The use of angiotensin-I converting enzyme (ACE) inhibitors is a common strategy for treating kidney disease. Several amino acid sequences with ACE inhibiting activity are identified in filet and rest raw material from various species of fish, and fish protein hydrolysates could be of interest for possible treatment or prevention of kidney disease. Therefore, we hypothesized that protein hydrolysates from rest raw material from herring and salmon contained ACE inhibiting motifs, and could beneficially affect typical markers for kidney function in an obesity rat model prone to developing renal failure...
April 2018: Nutrition Research
https://www.readbyqxmd.com/read/29757056/whey-protein-augments-leucinemia-and-post-exercise-p70s6k1-activity-compared-to-a-hydrolysed-collagen-blend-when-in-recovery-from-training-with-low-carbohydrate-availability
#3
Samuel G Impey, Kelly M Hammond, Robert Naughton, Carl Langan-Evans, Sam O Shepherd, Adam P Sharples, Jessica Cegielski, Kenneth Smith, Stewart Jeromson, D Lee Hamilton, Graeme L Close, James P Morton
We examined the effects of whey versus collagen protein on skeletal muscle cell signalling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate (CHO) availability. In a repeated measures design (after adhering to a 36-h exercise-dietary intervention to standardise pre-exercise muscle glycogen), eight males completed a 75-min non-exhaustive cycling protocol and consumed 22 g of a hydrolysed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise and 22 g immediately post-exercise...
May 14, 2018: International Journal of Sport Nutrition and Exercise Metabolism
https://www.readbyqxmd.com/read/29755300/the-effect-of-gel-microstructure-on-simulated-gastric-digestion-of-protein-gels
#4
Mauricio Opazo-Navarrete, Marte D Altenburg, Remko M Boom, Anja E M Janssen
The objective of this study was to analyse the impact of the gel structure obtained by different heat-induced temperatures on the in vitro gastric digestibility at pH 2. To achieve this, gels were prepared from soy protein, pea protein, albumin from chicken egg white and whey protein isolate at varying temperatures (90, 120 and 140 °C) for 30 min. Gels were characterised prior to digestion via microstructure and SDS-PAGE analysis. Subsequently, the gastric digestion process was followed via the protein hydrolysis and HPSEC analysis up to 180 min...
2018: Food Biophysics
https://www.readbyqxmd.com/read/29753488/gastric-digestion-of-milk-protein-ingredients-study-using-an-in-vitro-dynamic-model
#5
Xin Wang, Aiqian Ye, Quanquan Lin, Jianzhong Han, Harjinder Singh
The coagulation behavior and the kinetics of protein hydrolysis of skim milk powder, milk protein concentrate (MPC), calcium-depleted MPC, sodium caseinate, whey protein isolate (WPI), and heated (90°C, 20 min) WPI under gastric conditions were examined using an advanced dynamic digestion model (i.e., a human gastric simulator). During gastric digestion, these protein ingredients exhibited various pH profiles as a function of the digestion time. Skim milk powder and MPC, which contained casein micelles, formed cohesive, ball-like curds with a dense structure after 10 min of digestion; these curds did not disintegrate over 220 min of digestion...
May 9, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29752998/effect-of-protein-intake-beyond-habitual-intakes-following-resistance-training-on-cardiometabolic-risk-disease-parameters-in-pre-conditioned-older-women
#6
Rodrigo R Fernandes, Hellen C G Nabuco, Paulo Sugihara Junior, Edilaine F Cavalcanti, Crisieli M Tomeleri, Alex S Ribeiro, Décio S Barbosa, Danielle Venturini, Brad J Schoenfeld, Edilson S Cyrino
OBJECTIVE: The aim of this study was to analyze the effects of higher protein (HP) intake associated with resistance training (RT) on parameters of cardiometabolic risk disease (CMR). METHODS: A randomized, double-blind and placebo-controlled design was conducted. Thirty-two pre-conditioned older women were randomized in two groups to receive 35 g of protein (high protein group - HP) or 35 g of placebo (low protein group - LP) after training sessions. The RT program was carried out over 12 weeks, 3 days per week and 3 sets of 8-12 repetitions...
May 9, 2018: Experimental Gerontology
https://www.readbyqxmd.com/read/29751918/transglutaminase-mediated-microencapsulation-of-sea-buckthorn-supercritical-co-2-extract-in-whey-protein-isolate-and-valorization-in-highly-value-added-food-products
#7
Liliana Mihalcea, Mihaela Turturică, Vasilica Barbu, Elena Ioniţă, Livia Pătraşcu, Mihaela Cotârleţ, Loredana Dumitraşcu, Iuliana Aprodu, Gabriela Râpeanu, Nicoleta Stănciuc
Sea buckthorn carotenoids extracted using CO2 supercritical fluids method were encapsulated within whey proteins isolate by transglutaminase (TG) mediated crosslinking reaction, coacervation and freeze drying. The encapsulation efficiency was 36.23 ± 1.58%, with β-carotene the major carotenoid present in the powder. The confocal analysis revealed that TG-ase mediated cross-linking reaction enhanced the complexes stability to such a manner that a double microencapsulation was performed. The powder showed an antioxidant activity of 2...
October 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29751507/effects-of-whey-protein-supplementation-pre-or-post-resistance-training-on-muscle-mass-muscular-strength-and-functional-capacity-in-pre-conditioned-older-women-a-randomized-clinical-trial
#8
Hellen C G Nabuco, Crisieli M Tomeleri, Paulo Sugihara Junior, Rodrigo R Fernandes, Edilaine F Cavalcante, Melissa Antunes, Alex S Ribeiro, Denilson C Teixeira, Analiza M Silva, Luís B Sardinha, Edilson S Cyrino
Aging is associated with sarcopenia and dynapenia, with both processes contributing to functional dependence and mortality in older adults. Resistance training (RT) and increased protein intake are strategies that may contribute to health improvements in older adults. Therefore, the aim was to investigate the effects of whey protein (WP) supplementation consumed either immediately pre- or post-RT on skeletal muscle mass (SMM), muscular strength, and functional capacity in pre-conditioned older women. Seventy older women participated in this investigation and were randomly assigned to one of three groups: whey protein pre-RT and placebo post-RT (WP-PLA, n = 24), placebo pre-RT and whey protein post-RT (PLA-WP, n = 23), and placebo pre- and post-RT (PLA-PLA, n = 23)...
May 3, 2018: Nutrients
https://www.readbyqxmd.com/read/29749517/identification-of-a-5%C3%A2-lncrna-signature%C3%A2-based-risk-scoring-system-for-survival-prediction-in-colorectal-cancer
#9
Liqiang Gu, Jun Yu, Qing Wang, Bin Xu, Liechen Ji, Lin Yu, Xipeng Zhang, Hui Cai
The present study aimed to investigate potential prognostic long noncoding RNAs (lncRNAs) associated with colorectal cancer (CRC). An mRNA‑seq dataset obtained from The Cancer Genome Atlas was employed to identify the differentially expressed lncRNAs (DELs) between CRC patients with good and poor prognoses. Subsequently, univariate and multivariate Cox regression analyses were conducted to analyze the prognosis‑associated lncRNAs among all DELs. In addition, a risk scoring system was developed according to the expression levels of the prognostic lncRNAs, which was then applied to a training set and an independent testing set...
May 3, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29745984/effects-of-processing-on-structure-and-thermal-properties-of-powdered-preterm-infant-formula
#10
Xiaomeng Sun, Cuina Wang, Hao Wang, Mingruo Guo
Powdered infant formula is usually manufactured by ingredients mixing, homogenization, pasteurization, evaporation and spray drying. Effects of unit operations on the microstructure, thermal properties and other characteristics of preterm infant formula, fat (F), serum (S), and pellet (P) fractions on centrifugation were investigated using Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Differential Scanning Calorimetry (DSC), and Fourier Transform Infrared (FTIR) spectroscopy. After homogenization, particles which may be casein and denatured whey proteins were observed on the surface of F fraction in microstructure images...
May 10, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29735045/preparation-and-characterization-of-emulsion-filled-gel-beads-for-the-encapsulation-and-protection-of-resveratrol-and-%C3%AE-tocopherol
#11
Wenwen Feng, Chun Yue, Wusigale, Yingzhou Ni, Li Liang
Proteins and polysaccharides have been widely used as wall materials to prepare novel carriers for the encapsulation of a single bioactive component or co-encapsulation of different bioactive components. Emulsion-filled gel beads generally contain three essential components: oil droplets, interface membrane of emulsifier, and gel matrix. In this study, emulsion-filled gel beads were prepared by the combination of heat-denatured whey protein isolate (WPI)'s emulsification and alginate's gelation at various protein and oil contents, in order to discuss simultaneous encapsulation, protection and release of hydrophobic α-tocopherol and amphiphilic resveratrol...
June 2018: Food Research International
https://www.readbyqxmd.com/read/29735044/microparticulated-whey-protein-pectin-complex-a-texture-controllable-gel-for-low-fat-mayonnaise
#12
Chanchan Sun, Rui Liu, Bin Liang, Tao Wu, Wenjie Sui, Min Zhang
This article reports caloric value changes, stability and rheological properties of mayonnaises affected by fat mimetic based on Microparticulated whey protein (MWP) and high-methoxy pectin. Lipid was partially substituted at different levels of 20%, 40%, 60%, 80% and 100%, and the samples were referred to as FM20, FM40, FM60, FM80 and FFM, respectively. The full fat (FF) mayonnaise was used as a control experiment. For rheological properties, the addition of fat mimetic resulted in the gradual decrease of pseudoplastic behavior, relative thixotropic area and viscosity index, while elasticity index exhibited the opposite trend...
June 2018: Food Research International
https://www.readbyqxmd.com/read/29735042/influence-of-main-emulsion-components-on-the-physicochemical-and-functional-properties-of-w-o-w-nano-emulsion-effect-of-polyphenols-hi-cap-basil-seed-gum-soy-and-whey-protein-isolates
#13
Mojtaba Delfanian, Seyed M A Razavi, Mohammad Hossein Haddad Khodaparast, Reza Esmaeilzadeh Kenari, Shiva Golmohammadzadeh
In this study, the effect of natural macromolecules as carrier agents on the biological activity of nano-encapsulated Bene hull polyphenols (Pistacia atlantica subsp. Mutica) through W/O/W emulsions was evaluated. The W/O microemulsions as primary emulsions and a complex of soy protein isolate and basil seed gum (SPI-BSG), whey protein isolate and basil seed gum (WPI-BSG) and also Hi-Cap 100 in the outer aqueous phase were used to produce W/O/W nano-emulsions. Z-average size of emulsions stabilized by Hi-Cap, WPI-BSG, and SPI-BSG was 318, 736...
June 2018: Food Research International
https://www.readbyqxmd.com/read/29734122/reference-1d-and-2d-electrophoresis-maps-for-potential-disease-related-proteins-in-milk-whey-from-lactating-buffaloes-and-blood-serum-from-buffalo-calves-water-buffalo-bubalus-bubalis
#14
André M Santana, Funmilola C Thomas, Daniela G Silva, Eilidh McCulloch, Ana M C Vidal, Richard J S Burchmore, José J Fagliari, Peter D Eckersall
The aim of this study was to identify potential disease related proteins in milk whey of lactating buffaloes and blood serum of buffalo calves, in order to define a reference electrophoresis map for 1-DE and 2-DE. Additionally, changes in some protein patterns from buffalo calves during salmonellosis and lactating buffaloes during mastitis are presented. Milk samples were collected and distributed into groups: Milk samples from healthy buffaloes (SCC < 100.000 cells/ml, negative microbiology and CMT) (G1, n = 5) and buffaloes with subclinical mastitis (SCC > 500...
April 26, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29730930/quantitation-of-sialic-acids-in-infant-formulas-by-liquid-chromatography-mass-spectrometry-an-assessment-of-different-protein-sources-and-discovery-of-new-analogues
#15
Aaron Wylie, Wesley F Zandberg
Glycosidically-bound, but not free, dietary sialic acids are used for the biosynthesis of new glycoconjugates in humans, making the quantitation of these two forms in infant food sources important, as in neonates the demand for sialic acid may exceed the de novo biosynthetic supply. Here, a rapid high-performance liquid chromatography-mass spectrometry method was developed to identify and quantitate glycosidically-bound and free sialic acids in infant formulas. The sialic acid contents of eight commercially-available infant formulas with varying protein source or manufacturer were investigated...
May 6, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29724529/the-effect-of-daily-fortified-yogurt-consumption-on-weight-loss-in-adults-with-metabolic-syndrome-a-10-week-randomized-controlled-trial
#16
M Mohammadi-Sartang, N Bellissimo, J O Totosy de Zepetnek, N R Brett, S M Mazloomi, M Fararouie, A Bedeltavana, M Famouri, Z Mazloom
BACKGROUND AND AIMS: Obesity is a complex and multifaceted condition. Thus, functional foods need investigation as novel adjunct treatments for obesity. The objective was to determine the effects of daily consumption of a fortified yogurt (FY) on weight loss in overweight and obese patients with metabolic syndrome on a caloric-restricted diet. METHODS AND RESULTS: This was a randomized, double-blind, 10-week study. Participants were randomly allocated to two groups receiving either two servings (2 × 250 g)/day of FY with whey protein, calcium, vitamin D, prebiotic fibre and probiotic cultures (n = 44) or a low-fat plain yogurt (PY) (n = 43)...
March 10, 2018: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
https://www.readbyqxmd.com/read/29724127/growth-components-of-cow-s-milk-emphasis-on-effects-in-undernourished-children
#17
Benedikte Grenov, Kim F Michaelsen
BACKGROUND: Cow's milk is a core ingredient in foods for prevention and treatment of undernutrition in children. It promotes growth, but there is limited knowledge of which components in milk have growth-stimulating effects. OBJECTIVE: To discuss the growth-stimulating effects of milk in undernourished children with emphasis on protein, lactose, and minerals. METHODS: We reviewed literature on dairy and growth in undernourished children. RESULTS: Cow's milk has a specific stimulating effect on linear growth, even in well-nourished children, and it may stimulate weight gain and muscle accretion in wasted children...
January 1, 2018: Food and Nutrition Bulletin
https://www.readbyqxmd.com/read/29722584/no-difference-between-the-effects-of-supplementing-with-soy-protein-versus-animal-protein-on-gains-in-muscle-mass-and-strength-in-response-to-resistance-exercise
#18
Mark Messina, Heidi Lynch, Jared M Dickinson, Katharine E Reed
Much attention has been given to determining the influence of total protein intake and protein source on gains in lean body mass (LBM) and strength in response to resistance exercise training (RET). Acute studies indicate that whey protein, likely related to its higher leucine content, stimulates muscle protein synthesis (MPS) to a greater extent than proteins such as soy and casein. Less clear is the extent to which the type of protein supplemented impacts strength and LBM in longer term studies (≥6 weeks)...
May 3, 2018: International Journal of Sport Nutrition and Exercise Metabolism
https://www.readbyqxmd.com/read/29719871/a-prospective-metagenomic-and-metabolomic-analysis-of-the-impact-of-exercise-and-or-whey-protein-supplementation-on-the-gut-microbiome-of-sedentary-adults
#19
Owen Cronin, Wiley Barton, Peter Skuse, Nicholas C Penney, Isabel Garcia-Perez, Eileen F Murphy, Trevor Woods, Helena Nugent, Aine Fanning, Silvia Melgar, Eanna C Falvey, Elaine Holmes, Paul D Cotter, Orla O'Sullivan, Michael G Molloy, Fergus Shanahan
Many components of modern living exert influence on the resident intestinal microbiota of humans with resultant impact on host health. For example, exercise-associated changes in the diversity, composition, and functional profiles of microbial populations in the gut have been described in cross-sectional studies of habitual athletes. However, this relationship is also affected by changes in diet, such as changes in dietary and supplementary protein consumption, that coincide with exercise. To determine whether increasing physical activity and/or increased protein intake modulates gut microbial composition and function, we prospectively challenged healthy but sedentary adults with a short-term exercise regime, with and without concurrent daily whey protein consumption...
May 2018: MSystems
https://www.readbyqxmd.com/read/29717554/modification-of-immobead-150-support-for-protein-immobilization-effects-on-the-properties-of-immobilized-aspergillus-oryzae-%C3%AE-galactosidase
#20
Adriano Gennari, Francielle Herrmann Mobayed, Ruan da Silva Rafael, Rafael C Rodrigues, Raul Antonio Sperotto, Giandra Volpato, Claucia Fernanda Volken de Souza
We studied the modification of Immobead 150 support by either introducing aldehyde groups using glutaraldehyde (Immobead-Glu) or carboxyl groups through acid solution (Immobead-Ac) for enzyme immobilization by covalent attachment or ion exchange, respectively. These two types of immobilization were compared with the use of epoxy groups that are now provided on a commercial support. We used Aspergillus oryzae β-galactosidase (Gal) as a model protein, immobilizing it on unmodified (epoxy groups, Immobead-Epx) and modified supports...
May 1, 2018: Biotechnology Progress
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