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https://www.readbyqxmd.com/read/29349780/protective-effect-of-bled-exposed-conditioned-media-on-cell-injury
#1
Phil-Sun Oh, Eun-Mi Kim, Minjoo Kim, In Sun Kim, Yeon-Hee Han, SeokTae Lim, Myung-Hee Sohn, Myoung-Hwan Ko, Hwan-Jeong Jeong
Previous studies have reported that 450 nm blue light emitting diode (BLED) induces apoptosis through a mitochondria-mediated pathway in cancer cells and reduces the early stage tumor growth. The present study was performed to determine the effects of BLED-irradiated cell metabolites on cell injury. Our results showed that conditioned medium (CM) from cells irradiated with low-dose BLED (LCM) inhibited apoptosis and increased cell survival. Cell protection-related proteins were identified in cell metabolites of CM and LCM using 2-DE and MALDI-TOF analysis...
January 19, 2018: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/29332352/puerarin-leads-to-k562-cell-apoptosis-of-chronic-myelogenous-leukemia-via-induction-of-autophagy
#2
Da Gao, Zhen Xiao, Hui-Ping Li
PURPOSE: To study the effects of puerarin on the viability, apoptosis and autophagy of K562 cells of chronic myelogenous leukemia (CML), and to provide a basis for the study on antitumor mechanism of puerarin. METHODS: K562 cells of human CML were taken as the study material and puerarin was applied in different concentrations. The effect of puerarin on cell viability was detected via cholecystokinin-8 (CCK8) and lactate dehydrogenase (LDH). Flow cytometry and western blot (WB) were used to detect cell apoptosis, while Cyto-ID and WB were used to detect the cell autophagy level...
November 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/29328918/transmembrane-pickets-connect-cyto-and-pericellular-skeletons-forming-barriers-to-receptor-engagement
#3
Spencer A Freeman, Anthony Vega, Magdalena Riedl, Richard F Collins, Phillip P Ostrowski, Elliot C Woods, Carolyn R Bertozzi, Markku I Tammi, Diane S Lidke, Pauline Johnson, Satyajit Mayor, Khuloud Jaqaman, Sergio Grinstein
Phagocytic receptors must diffuse laterally to become activated upon clustering by multivalent targets. Receptor diffusion, however, can be obstructed by transmembrane proteins ("pickets") that are immobilized by interacting with the cortical cytoskeleton. The molecular identity of these pickets and their role in phagocytosis have not been defined. We used single-molecule tracking to study the interaction between Fcγ receptors and CD44, an abundant transmembrane protein capable of indirect association with F-actin, hence likely to serve as a picket...
January 11, 2018: Cell
https://www.readbyqxmd.com/read/29318096/a-two-photon-ratiometric-fluorescent-probe-for-imaging-of-hydrogen-peroxide-levels-in-rat-organ-tissues
#4
Chang Su Lim, Myoung Ki Cho, Mi Yeon Park, Hwan Myung Kim
Hydrogen peroxide (H2O2) is important in the regulation of a variety of biological processes and is involved in various diseases. Quantitative measurement of H2O2 levels at the subcellular level is important for understanding its positive and negative effects on biological processes. Herein, a two-photon ratiometric fluorescent probe (SHP-Cyto) with a boronate-based carbamate leaving group as the H2O2 reactive trigger and 6-(benzo[d]thiazol-2'-yl)-2-(N,N-dimethylamino) naphthalene (BTDAN) as the fluorophore was synthesized and examined for its ability to detect cytosolic H2O2 in situ...
January 2018: ChemistryOpen
https://www.readbyqxmd.com/read/29317239/protective-effect-of-mitochondrial-targeted-antioxidant-mitoq-against-iron-ion-56fe-radiation-induced-brain-injury-in-mice
#5
Lu Gan, Zhenhua Wang, Jing Si, Rong Zhou, Chao Sun, Yang Liu, Yancheng Ye, Yanshan Zhang, Zhiyuan Liu, Hong Zhang
Exposure to iron ion 56Fe radiation (IR) during space missions poses a significant risk to the central nervous system and radiation exposure is intimately linked to the production of reactive oxygen species (ROS). MitoQ is a mitochondria-targeted antioxidant that has been shown to decrease oxidative damage and lower mitochondrial ROS in a number of animal models. Therefore, the present study aimed to investigate role of the mitochondrial targeted antioxidant MitoQ against 56Fe particle irradiation-induced oxidative damage and mitochondria dysfunction in the mouse brains...
January 6, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29314656/mammalian-target-of-rapamycin-inhibition-attenuates-myocardial-ischaemia-reperfusion-injury-in-hypertrophic-heart
#6
Lei-Lei Ma, Xin Ma, Fei-Juan Kong, Jun-Jie Guo, Hong-Tao Shi, Jian-Bing Zhu, Yun-Zeng Zou, Jun-Bo Ge
Pathological cardiac hypertrophy aggravated myocardial infarction and is causally related to autophagy dysfunction and increased oxidative stress. Rapamycin is an inhibitor of serine/threonine kinase mammalian target of rapamycin (mTOR) involved in the regulation of autophagy as well as oxidative/nitrative stress. Here, we demonstrated that rapamycin ameliorates myocardial ischaemia reperfusion injury by rescuing the defective cytoprotective mechanisms in hypertrophic heart. Our results showed that chronic rapamycin treatment markedly reduced the phosphorylated mTOR and ribosomal protein S6 expression, but not Akt in both normal and aortic-banded mice...
January 4, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29305910/the-development-of-brain-white-matter-microstructure
#7
Catherine Lebel, Sean Deoni
Throughout infancy, childhood, and adolescence, our brains undergo remarkable changes. Processes including myelination and synaptogenesis occur rapidly across the first 2-3 years of life, and ongoing brain remodeling continues into young adulthood. Studies have sought to characterize the patterns of structural brain development, and early studies predominately relied upon gross anatomical measures of brain structure, morphology, and organization. MRI offers the ability to characterize and quantify a range of microstructural aspects of brain tissue that may be more closely related to fundamental neurodevelopmental processes...
January 3, 2018: NeuroImage
https://www.readbyqxmd.com/read/29286143/epithelial-mesenchymal-transition-induced-by-the-cxcl9-cxcr3-axis-through-akt-activation-promotes-invasion-and-metastasis-in-tongue-squamous-cell-carcinoma
#8
Ziliang Li, Jianqi Liu, Lei Li, Shuai Shao, Jianhua Wu, Li Bian, Yongwen He
The present study aimed to assess the induction of epithelial-mesenchymal transition (EMT), invasion, and metastasis by the chemokine CXCL9/receptor CXCR3 axis in tongue squamous cell carcinoma (TSCC), unveiling the underlying mechanisms and providing new insights into the prevention and treatment of oral cancer metastasis. The expression levels of CXCL9 and CXCR3 in TSCC tissue specimens were determined by immunohistochemistry, assessing differences between samples with cervical lymph node metastasis and those without...
December 20, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29273330/monitoring-of-morphotoxic-cytotoxic-and-genotoxic-potential-of-mancozeb-using-allium-assay
#9
Firdos Fatma, Sonam Verma, Aisha Kamal, Alka Srivastava
The present experiment was designed to monitor the morphotoxic, cytotoxic and genotoxic potential of Mancozeb (fungicide) in non-target plants using bulbs of Allium cepa. Mancozeb is classified as a contact fungicide and is registered for use on a variety of crop plants. In the present monitoring, Allium cepa bulbs were exposed to different concentrations of mancozeb viz., 10, 30, 50, 70, 90, 110, 130 and 150 ppm for 24 and 48 h. The potential morphotoxic and cytotoxic effects of mancozeb were examined by determining the average root number, average root length, mitotic index, relative abnormality rate (%) and frequency of abnormalities (%)...
December 19, 2017: Chemosphere
https://www.readbyqxmd.com/read/29273065/curcumin-synergizes-with-5-fluorouracil-by-impairing-ampk-ulk1-dependent-autophagy-akt-activity-and-enhancing-apoptosis-in-colon-cancer-cells-with-tumor-growth-inhibition-in-xenograft-mice
#10
Pan Zhang, Ze-Lin Lai, Hui-Fen Chen, Min Zhang, An Wang, Tao Jia, Wen-Qin Sun, Xi-Min Zhu, Xiao-Feng Chen, Zheng Zhao, Jun Zhang
BACKGROUND: Chemoresistance is a major obstacle that limits the benefits of 5-Fluorouracil (5-Fu)-based chemotherapy for colon cancer patients. Autophagy is an important cellular mechanism underlying chemoresistance. Recent research advances have given new insights into the use of natural bioactive compounds to overcome chemoresistance in colon cancer chemotherapy. As one of the multitargeted and safer phytomedicines, curcumin has been reported to work as cancer-specific chemosensitizer, presumably via induction of autophagic signaling pathways...
December 22, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29258234/time-dependent-toxic-and-genotoxic-effects-of-zinc-oxide-nanoparticles-after-long-term-and-repetitive-exposure-to-human-mesenchymal-stem-cells
#11
Pascal Ickrath, Martin Wagner, Agmal Scherzad, Thomas Gehrke, Marc Burghartz, Rudolf Hagen, Katrin Radeloff, Norbert Kleinsasser, Stephan Hackenberg
Zinc oxide nanoparticles (ZnO-NP) are widely spread in consumer products. Data about the toxicological characteristics of ZnO-NP is still under controversial discussion. The human skin is the most important organ concerning ZnO-NP exposure. Intact skin was demonstrated to be a sufficient barrier against NPs; however, defect skin may allow NP contact to proliferating cells. Within these cells, stem cells are the most important toxicological target for NPs. The aim of this study was to evaluate the genotoxic and cytotoxic effects of ZnO-NP at low-dose concentrations after long-term and repetitive exposure to human mesenchymal stem cells (hMSC)...
December 18, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29248806/magnesium-alloy-based-interference-screw-developed-for-acl-reconstruction-attenuates-peri-tunnel-bone-loss-in-rabbits
#12
Jiali Wang, Yuanhao Wu, Huafang Li, Yang Liu, Xueling Bai, Wingho Chau, Yufeng Zheng, Ling Qin
Peri-tunnel bone loss after anterior cruciate ligament (ACL) reconstruction is often observed clinically, which may detrimentally affect tendon graft integration with surrounding bone tissue. Biodegradable magnesium (Mg) based fixators in terms of interference screws may be suitable for fixation of the tendon graft due to their favorable effects on promotion of new bone formation. However, the poor mechanical strength of Mg is still one of the major challenges for its clinical applications. The addition of alloying elements into Mg is one of the strategies to improve their mechanical properties...
December 11, 2017: Biomaterials
https://www.readbyqxmd.com/read/29232723/biopsy-of-liver-target-lesions-under-contrast-enhanced-ultrasound-guidance-a-multi-center-study
#13
Giampiero Francica, Maria Franca Meloni, Ilario de Sio, Fulvia Terracciano, Eugenio Caturelli, Laura Riccardi, Paola Roselli, Maddalena Diana Iadevaia, Mariano Scaglione, Giovanni Lenna, Jason Chiang, Maurizio Pompili
Purpose To retrospectively characterize the prevalence and impact of contrast-enhanced ultrasound (CEUS) as a guidance technique for the biopsy of liver target lesions (LTLs) at six interventional ultrasound centers. Materials and Methods The six participating centers retrospectively selected all patients in whom biopsy needles were positioned in LTLs during CEUS. The prevalence of CEUS-guided biopsies at each center between 2005 and 2016, contrast agent consumption, procedure indications, diagnostic yield and complications were assessed...
December 12, 2017: Ultraschall in der Medizin
https://www.readbyqxmd.com/read/29228170/biotransformation-of-trinitrotoluene-by-citrobacter-sp-yc4-and-evaluation-of-its-cyto-toxicological-effects
#14
Chih-Ming Kao, Shih-Feng Wei, Ssu Ching Chen, Chao-Ling Yao, Cheng Ma, Chih-Ching Chien
Trinitrotoluene (TNT) is an explosive chemical generally used for military, civil and industrial purposes. Therefore, TNT residue can be found in soil and ground water as an environmental pollutant. The environmental control of TNT pollution has become a critical issue because of its potential toxicity and carcinogenicity. The aim of this study is to evaluate the cyto-toxicological effects of TNT after bioremediation. Citrobacter sp. YC4 is able to utilize TNT as a sole nitrogen source. Citrobacter sp. YC4 cells grown in medium with TNT as the sole nitrogen source (TNT-N) were able to rapidly degrade TNT, in contrast to cells grown in Luria Bertani medium as determined by resting cell suspension...
December 6, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29221728/emulsified-isoflurane-induces-release-of-cytochrome-c-in-human-neuroblastoma-shsy-5y-cells-via-jnk-c-jun-n-terminal-kinases-signaling-pathway
#15
Zeyong Yang, Jingjing Lv, Xianfu Lu, Xingxing Li, Xiaohu An, Jianwei Wang, Hao Weng, Yuanhai Li
A large number of studies have demonstrated that inhalation anesthetic isoflurane induced neural cell death by apoptosis in various cell and animal models. Emulsified isoflurane (EIso) is a new type of intravenous preparation of isoflurane that attracts increasing research attention as a promising clinical agent due to its both advantages as an intravenous and inhalation anesthetics medication. However, its safety and underlying molecular mechanism of neurotoxicity largely remain unknown. Therefore, it is meaningful to investigate the safety of EIso and to further elucidate its mechanism of anesthetic neurotoxicity...
December 5, 2017: Neurotoxicology and Teratology
https://www.readbyqxmd.com/read/29220288/contemporary-sarcoma-diagnosis-genetics-and-genomics
#16
Inga-Marie Schaefer, Gregory M Cote, Jason L Hornick
Sarcomas include diverse mesenchymal neoplasms with widely varied prognosis, clinical behavior, and treatment. Owing to their rarity and histologic overlap, accurate diagnosis of sarcomas can be challenging. Our approach has evolved dramatically in the past few decades, where novel insights into the molecular pathogenetic basis for sarcomas has dramatically (re)shaped contemporary diagnosis, building on a largely morphology- and clinical presentation-based strategy. Examples include the introduction of novel immunohistochemical markers that serve as surrogates for molecular genetic alterations and identification of characteristic molecular alterations...
December 8, 2017: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/29214554/geniposide-suppresses-interleukin-1%C3%AE-induced-inflammation-and-apoptosis-in-rat-chondrocytes-via-the-pi3k-akt-nf-%C3%AE%C2%BAb-signaling-pathway
#17
Tianlong Pan, Xuchao Shi, Huan Chen, Rong Chen, Dengying Wu, Zeng Lin, Jingdong Zhang, Jun Pan
Osteoarthritis (OA) is a chronic degenerative joint disease that is principally characterized by progressive joint dysfunction and cartilage degradation. Inflammation and apoptosis play critical roles in the progression of OA. Geniposide (GPO), one of the principal components of the fruit of Gardenia jasminoides Ellis, has been reported to have anti-inflammatory and other pharmacological effects. In this study, we performed in vitro experiments on rat chondrocytes to examine the therapeutic effects of GPO on OA and investigated its effects in vivo in a rat model of OA induced by medial meniscal tear (MMT)...
December 6, 2017: Inflammation
https://www.readbyqxmd.com/read/29211455/a-mrna-initiated-three-dimensional-dna-amplifier-able-to-function-inside-living-cells
#18
Lei He, Danqing Lu, Hao Liang, Sitao Xie, Xiaobing Zhang, Qiaoling Liu, Quan Yuan, Weihong Tan
DNA molecular machines show great promise in fields like biomarker discovery and biological activity regulation, but operating DNA machines with specific functions within living systems remains extremely challenging. Although DNA machines have been engineered with exact molecu-lar-level specifications, some intrinsic imperfections such as poor cell permeation and fragility in complex cyto-plasmic milieu persist due to the well-established charac-ter of nucleic acid molecules. To circumvent these prob-lems, we herein report a molecularly engineered, entropy-driven three-dimensional DNA amplifier (EDTD) that can operate inside living cells in response to a specific mRNA target...
December 6, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29204990/apoptosis-induction-of-colorectal-cancer-cells-htl-9-in-vitro-by-the-transformed-products-of-soybean-isoflavones-by-ganoderma-lucidum
#19
Mei-Lin Cui, Huan-Yi Yang, Guo-Qing He
Soybean isoflavones have been one of the potential preventive candidates for antitumor research in recent years. In this paper, we first studied the transformation of soybean isoflavones with the homogenized slurry of Ganoderma lucidum. The resultant transformed products (TSI) contained (703.21±4.35) mg/g of genistein, with transformed rates of 96.63% and 87.82% of daidzein and genistein, respectively, and TSI also could enrich the bioactive metabolites of G. lucidum. The antitumor effects of TSI on human colorectal cancer cell line HTL-9, human breast cancer cell line MCF-7, and human immortalized gastric epithelial cell line GES-1 were also studied...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/29202290/insights-about-the-toxic-effects-of-tannery-effluent-on-lithobates-catesbeianus-tadpoles
#20
Diogo Ferreira do Amaral, Mateus Flores Montalvão, Bruna de Oliveira Mendes, Joyce Moreira de Souza, Thales Quintão Chagas, Aline Sueli de Lima Rodrigues, Guilherme Malafaia
Tannery industries are considered highly polluting due to the large production of polluted wastewater [untreated tannery effluent (UTE)]. Although previous studies have already shown the consequences from fish, birds and mammals' exposure to this pollutant, little is known about its toxicological effect on representatives of class amphibian. Thus, we aim at assessing whether short-term UTE exposure, even at low concentrations (0.2% UTE diluted in water), would be able to induce behavioral, morphological and cyto-genotoxic changes in L...
December 1, 2017: Science of the Total Environment
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