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Ya-Wei Li, Wei Zhang, Na Xu, Yan Li, Hong Zhang, Shi-Jie Lv, Wen-He Zhu
To investigate the effect of dihydroartemisinin on apoptosis of human pancreatic cancer cell line JF-305 and the role of reactive oxygen species(ROS) in the apoptosis of JF-305 cells induced by dihydroartemisinin. MTT assays were used to detect effect of different concentrations of dihydroartemisinin on cells proliferation of JF-305 lines. Cell cycle was detected by flow cytometry, and the apoptotic morphology was observed by Hoechst 333258 fluorescence staining. Annexin V fluorescence staining was used to detect the apoptosis changes of JF-305 cells, while DCFH-DA was used to detect the changes of ROS during apoptosis process...
August 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Marthandam Asokan Shibu, Chia-Hua Kuo, Bih-Cheng Chen, Da-Tong Ju, Ray-Jade Chen, Chao-Hung Lai, Pei-Jane Huang, Vijaya Padma Viswanadha, Wei-Wen Kuo, Chih-Yang Huang
Tea, the most widely consumed natural beverage has been associated with reduced mortality risk from cardiovascular disease. Oolong tea is a partially fermented tea containing high levels of catechins, their degree of oxidation varies between 20%-80% causing differences in their active metabolites. In this study we examined the effect of oolong tea extract (OTE) obtained by oxidation at low-temperature for short-time against hypoxic injury and found that oolong tea provides cyto-protective effects by suppressing the JNK mediated hypertrophic effects and by enhancing the innate antioxidant mechanisms in neonatal cardiomyocytes and in H9c2 cells...
November 15, 2017: Environmental Toxicology
O Bogado Pascottini, M Hostens, G Opsomer
This study aimed to (i) assess the prevalence of cytological endometritis (CYTO) diagnosed at artificial insemination (AI); (ii) evaluate the effect of CYTO on the pregnancy outcome of the same AI sample; and (iii) determine the risk factors associated with CYTO diagnosed at AI in repeat breeder (RB) dairy cows. We analysed the productive and reproductive performances of 146 RB Holstein-Friesian cows. To obtain a CYTO sample at AI, we used the cytotape technique. Generalized mixed effect models were computed to find the risk factors associated with the pregnancy and CYTO outcome...
November 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
Edison Bezerra da Silva, Sandra Aparecida da Silva Corrêa, Denis Moledo de Souza Abessa, Bruno Ferreira Xavier da Silva, Dolores Helena Rodriguez Ferreira Rivero, Robson Seriani
The present study evaluated the water quality of a polluted pond through the analysis of in vitro mucociliary transport, hematological parameters, and biomarkers of cyto-genotoxicity in the Nile tilapia (Oreochromis niloticus). Blood and mucus samples were collected from ten specimens from the polluted pond and from ten specimens from a control area. The fish were anesthetized with 3% benzocaine, mucus was collected directly from the gills, and blood was drawn from the caudal artery. Blood smears were stained using the May-Grünwald Giemsa process for the differential leukocyte counts and to determine the frequency of leukocytes, thrombocytes, erythroblasts, micronuclei, and nuclear abnormalities...
November 13, 2017: Environmental Science and Pollution Research International
Pradeep Dheerendra, Nicholas M Lynch, Joseph Crutwell, Mark O Cunningham, Tom V Smulders
Avian and mammalian brains have evolved independently from each other for about 300 million years. During that time, the hippocampal formation (HF) has diverged in morphology and cyto-architecture, but seems to have conserved much of its function. It is therefore an open question how seemingly different neural organizations can generate the same function. A prominent feature of the mammalian hippocampus is that it generates different neural oscillations, including the gamma rhythm, which plays an important role in memory processing...
November 9, 2017: European Journal of Neuroscience
Khuraijam Bembem, Ankita Jaiswal, Meeta Singh, Nidhi Verma, Shyama Jain, Anusha Bhat
Superficial angiomyxoma (SA) is one of the superficially located myxoid soft tissue tumor. Because of the rarity of this tumor, there is paucity of fine needle aspiration cytology (FNAC) findings. Here, we present a case of SA in a 14-year-old girl who presented with a left leg swelling. The cytology of the lesion along with the histopathologic correlation is presented.
October 2017: Journal of Cytology
Stefan W Ryter, Kevin C Ma, Augustine M K Choi
Carbon monoxide (CO) is an endogenously produced gas that has gained recognition as a biological signal transduction effector with properties similar, but not identical, to that of nitric oxide (NO). CO, which binds primarily to heme iron, may activate the hemoprotein guanylate cyclase (sGC), although with lower potency than NO. Furthermore, CO can modulate the activities of several cellular signaling molecules such as p38 MAPK, ERK1/2, JNK, AKT, NF-κB, and others. Emerging studies suggest that mitochondria, the energy-generating organelle of cells, represent a key target of CO action in eukaryotes...
November 8, 2017: American Journal of Physiology. Cell Physiology
Yu Wang, Chunhui Xia, Zhiqiang Lun, Yanxin Lv, Wei Chen, Tao Li
Photodynamic therapy (PDT) is considered to be an advancing antitumor technology. PDT using hydrophilic/lipophilic tetra‑α-(4-carboxyphenoxy) phthalocyanine zinc (TαPcZn-PDT) has exhibited antitumor activity in Bel-7402 hepatocellular cancer cells. However, the manner in which p38 MAPK and caspase-9 are involved in the regulation of mitochondria-mediated apoptosis in the TαPcZn-PDT-treated LoVo human colon carcinoma cells remains unclear. Therefore, in the present study, a siRNA targeting p38 MAPK (siRNA-p38 MAPK) and the caspase‑9 specific inhibitor z-LEHD-fmk were used to examine the crosstalk between p38 MAPK and caspase-9 during mitochondria-mediated apoptosis in the TαPcZn-PDT‑treated LoVo cells...
November 2, 2017: Oncology Reports
Wei Zhang, Shihua Zhang, Meiling Zhang, Lige Yang, Baojing Cheng, Jianping Li, Anshan Shan
Deoxynivalenol (DON), zearalenone (ZEN) and fumonisin B1 (FB1) are among the most toxicologically important Fusarium toxins commonly found in nature that lead to nephrotoxicity in animals. The present study investigated that the individual and combined effects of subcytotoxic DON (0.25 μM), ZEN (20 μM) and FB1 (10 μM) on oxidative stress and apoptosis in porcine kidney cells (PK15). In addition, the protective effect of N-acetylcysteine (NAC) against the toxicity of Fusarium toxins was also evaluated...
November 7, 2017: Food and Chemical Toxicology
Goran Gajski, Carina Ladeira, Marko Gerić, Vera Garaj-Vrhovac, Susana Viegas
Cytostatic drugs are highly cytotoxic agents used in cancer treatment and although their benefit is unquestionable, they have been recognized as hazardous to healthcare professionals in occupational settings. In a working environment, simultaneous exposure to cytostatics may occur creating a higher risk than that of a single substance. Hence, the present study evaluated the combined cyto/genotoxicity of a mixture of selected cytostatics with different mechanisms of action (MoA; 5-fluorouracil, cyclophosphamide and paclitaxel) towards human lymphocytes in vitro at a concentration range relevant for occupational as well as environmental exposure...
October 31, 2017: Environmental Research
Nils Eide, Ragnar S Faye, Hanne K Høifødt, Leiv Sandvik, Geir A Qvale, Rowan Faber, Peter Jebsen, Gunnar Kvalheim, Øystein Fodstad
Approximately 50% of uveal melanoma patients develop metastases. We want to evaluate the effect of stricter criteria on our data from our previous study correlating survival and bone marrow (BM) micrometastasis results using our immunomagnetic separation (IMS) method. Mononuclear cell fractions (MNC) isolated from BM were examined for tumour cells and the patients were classified as BM positive (BM+) or BM negative (BM-). The study originally included 328 consecutive patients with uveal melanoma from 1997 to 2006...
November 2, 2017: Pathology Oncology Research: POR
Sónia Silva, Sandra C Craveiro, Helena Oliveira, António J Calado, Ricardo J B Pinto, Artur M S Silva, Conceição Santos
This study investigates the phytotoxicity of chronic exposure (up to 20 d) of different TiO2 nanoparticles (TiO2-NP) concentrations (5, 50, 150 mg L(-1)) in Triticum aestivum. Germination was not affected by TiO2-NP exposure and seedling shoot length (3 d) was enhanced. Contrarily, plants' shoot growth (20 d) was impaired. Effects on membrane permeability and total antioxidant capacity in TiO2-NP chronic exposure were organ dependent: increased in leaves and decreased in roots. Roots also showed lower levels of lipid peroxidation...
October 19, 2017: Plant Physiology and Biochemistry: PPB
Elena Hollergschwandtner, Thomas Schwaha, Josef Neumüller, Ulrich Kaindl, Daniela Gruber, Margret Eckhard, Michael Stöger-Pollach, Siegfried Reipert
Background: Biomineralization, e.g., in sea urchins or mollusks, includes the assembly of mesoscopic superstructures from inorganic crystalline components and biopolymers. The resulting mesocrystals inspire biophysicists and material scientists alike, because of their extraordinary physical properties. Current efforts to replicate mesocrystal synthesis in vitro require understanding the principles of their self-assembly in vivo. One question, not addressed so far, is whether intracellular crystals of proteins can assemble with biopolymers into functional mesocrystal-like structures...
2017: PeerJ
A Mahdee, J Eastham, J M Whitworth, J I Gillespie
OBJECTIVE: To re-examine the morphology and potential functions of odontoblasts in intact rat incisors and after cavity preparation into dentine. DESIGN: Intact incisors were fixed, decalcified, snap frozen and sectioned (10μm), before staining with rhodamine phalloidin or antibodies for cyto-skeletal proteins: vimentin and actin, ion transporter: NaK-ATPase, and dendritic cell marker: OX6. Samples with cavity were processed similarly and stained for actin and vimentin before comparing the lengths of odontoblast processes (OP) at baseline, 3h and 24h (n=5 for each group)...
October 10, 2017: Archives of Oral Biology
Yuyun Yang, Kai Zheng, Ruifang Liang, Astrid Mainka, Nicola Taccardi, Judith A Roether, Rainer Detsch, Wolfgang H Goldmann, Sannakaisa Virtanen, Aldo R Boccaccini
Bioactive glass nanoparticles containing copper (Cu-BGNs) were introduced into polycaprolactone (PCL) coating systems to improve the bioactivity, antibacterial properties, and corrosion resistance of vulnerable magnesium matrices under physiological conditions. The influence of different amounts of Cu-BGNs in PCL coatings was thoroughly investigated in determining the wettability, electrochemical properties, and antibacterial effects against Staphylococcus carnosus and Escherichia coli, as well as their cyto-compatibility...
October 26, 2017: Biomedical Materials
Juliana Guarize, Monica Casiraghi, Stefano Donghi, Chiara Casadio, Cristina Diotti, Niccolò Filippi, Clementina Di Tonno, Valeria Midolo, Patrick Maisonneuve, Daniela Brambilla, Chiara Maria Grana, Francesco Petrella, Lorenzo Spaggiari
Mediastinal lymph node enlargement is common in the follow-up of patients with previously treated malignancies. The aim of this study is to assess the role of endobronchial ultrasound (EBUS) transbronchial needle aspiration (TBNA) for cyto-histological evaluation of positron emission tomography with (18)fluorodeoxyglucose (PET) positive mediastinal and hilar lymph nodes developed in patients with previous malignancies. All EBUS-TBNA cases performed from January 2012 to May 2016 were retrospective reviewed. Results of EBUS-TBNA in patients with mediastinal and/or hilar lymphadenopathies were analysed...
October 2017: ERJ Open Research
Simone Brivio, Massimiliano Cadamuro, Luca Fabris, Mario Strazzabosco
The acquisition of invasive functions by tumor cells is a first and crucial step towards the development of metastasis,which nowadays represents the main cause of cancer-related death. Cholangiocarcinoma (CCA), a primary liver cancer originating from the biliary epithelium, typically develops intrahepatic or lymph node metastases at early stages, thus preventing the majority of patients from undergoing curative treatments, consistent with their very poor prognosis. As in most carcinomas, CCA cells gradually adopt a motile, mesenchymal-like phenotype, enabling them to cross the basement membrane, detach from the primary tumor, and invade the surrounding stroma...
October 25, 2017: Gene Expression
Christina Herold, Christina Paulitschek, Nicola Palomero-Gallagher, Onur Güntürkün, Karl Zilles
At the beginning of the 20(th) century it was suggested that a complex group of nuclei in the avian posterior ventral telencephalon is comparable to the mammalian amygdala. Subsequent findings, however, revealed that most of these structures share premotor characteristics, while some indeed constitute the avian amygdala. These developments resulted in 2004 in a change of nomenclature of these nuclei, which from then on were named arcopallial or amygdala nuclei and referred to as the arcopallium/amygdala complex...
October 24, 2017: Journal of Comparative Neurology
Dae Hwan Shin, Glen S Kwon
Despite clinical remission of epithelial ovarian cancer (EOC) after surgical resection and first-line chemotherapy, about 60% of patients will re-develop peritoneal metastasis and about 50% will relapse with chemoresistant disease. Clinical studies suggest that intra-peritoneal (i.p.) chemotherapy effectively treats residual EOC after cyto-reduction by gaining direct access into the peritoneal cavity, enabling elevated drug levels versus intravenous (i.v.) injection. However, chemoresistant disease is still problematic...
October 19, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
Vignesh S, Sivashanmugam A, Mohandas Annapoorna, Janarthanan R, Iyer Subramania, Nair Shantikumar V, Jayakumar R
In this study, an injectable chitosan-hyaluronic acid (CS-HA) based hydrogel was designed incorporating pro-angiogenic molecule, deferoxamine loaded PLGA nanoparticles (DFO NPs), for enhancing angiogenesis. DFO-NPs were prepared by double emulsion solvent diffusion technique and characterized for their physicochemical properties. The DLS and SEM analysis showed an average particle size of 220±71nm with spherical morphology and the encapsulation efficiency was found to be 30±5%. An ECM mimicking chitosan-hyaluronic acid (CS-HA) coacervate hydrogel was prepared...
October 12, 2017: Colloids and Surfaces. B, Biointerfaces
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