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https://www.readbyqxmd.com/read/28524813/a-multifunctional-nanocomplex-for-enhanced-cell-uptake-endosomal-escape-and-improved-cancer-therapeutic-effect
#1
Patrick V Almeida, Mohammad-Ali Shahbazi, Alexandra Correia, Ermei Mäkilä, Marianna Kemell, Jarno Salonen, Jouni Hirvonen, Hélder A Santos
AIM: To evaluate the chemotherapeutic potential of a novel multifunctional nanocomposite encapsulating both porous silicon (PSi) and gold (Au) nanoparticles in a polymeric nanocomplex. MATERIALS & METHODS: The nanocomposite was physicochemically characterized and evaluated in vitro for biocompatibility, cellular internalization, endosomolytic properties, cytoplasmatic drug delivery and chemotherapeutic efficacy. RESULTS: The nanocomposites were successfully produced and exhibited adequate physicochemical properties and superior in vitro cyto- and hemocompatibilities...
May 19, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28510114/fabricating-poly-1-8-octanediol-citrate-elastomer-based-fibrous-mats-via-electrospinning-for-soft-tissue-engineering-scaffold
#2
Lei Zhu, Yuanzheng Zhang, Yali Ji
Poly(1,8-octanediol citrate) (POC) is a recently developed biodegradable crosslinked elastomer that possesses good cytocompatibility and matchable mechanical properties to soft tissues. However, the thermosetting characteristic reveals a big challenge to manufacture its porous scaffold. Herein, POC elastomer was electrospun into fiber mat using poly(L-lactic acid) (PLLA) as a spinnable carrier. The obtained POC/PLLA fiber mats were characterized by scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), uniaxial tensile test, static-water-contact-angle, thermal analysis, in vitro degradation and biocompatibility test...
June 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28506752/thio-functionalized-carbohydrate-thiosemicarbazones-and-evaluation-of-their-anticancer-activity
#3
Anna Czubatka-Bieńkowska, Joanna Sarnik, Anna Macieja, Grzegorz Galita, Zbigniew J Witczak, Tomasz Poplawski
Thiosemicarbazides and their analogs have shown potential medical applications as antiviral, antibacterial and anticancer drugs. We designed, synthesized and evaluated in vitro anticancer activity against ovarian (A2780), cervix (HeLa), colon (LoVo), breast (MCF-7) and brain (MO59J) human cancer cell lines of seven novel compounds -S-glycosylated thiosemicarbazones. We assessed the cyto- and genotoxic properties of all novel compounds using a variety of methods including comet assay, XTT assay, various fluorescent assays and toxicology PathwayFinder expression array...
April 21, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28496485/phytochemicals-antioxidant-and-antiproliferative-properties-of-rosmarinus-officinalis-l-on-u937-and-caco-2-cells
#4
Yacine Amar, Boumediene Meddah, Irene Bonacorsi, Gregorio Costa, Gaetana Pezzino, Antonina Saija, Mariateresa Cristani, Soulef Boussahel, Guido Ferlazzo, Aicha Tirtouil Meddah
Rosmarinus officinalis L., a medicinal herb from the labiates family, has been reported to have potential benefit in the treatment and prevention of several diseases. In particular its phenolics have demonstrated protective effects on various types of cancer through several mechanisms. The present study aimed to determine the effects of rosemary phenolic extracts on human cell functions, with particular regard to their anti-proliferative properties in three cell types U937, CaCo-2 and the peripheral blood mononuclear cells (PBMCs)...
2017: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/28496454/expression-analyses-of-embryogenesis-associated-genes-during-somatic-embryogenesis-of-adiantum-capillus-veneris-l-in-vitro-new-insights-into-the-evolution-of-reproductive-organs-in-land-plants
#5
Xia Li, Jing-Dan Han, Yu-Han Fang, Shu-Nong Bai, Guang-Yuan Rao
An efficient in vitro regeneration system via somatic embryogenesis (SE) was developed for a fern species Adiantum capillus-veneris. Adventitious shoots, green globular bodies (GGBs) and calli were obtained with the maximal induction rate on the Murashige and Skoog (MS) medium of low concentrations of 6-benzyladenine (BA) (0-1.0 mg/L), 2.0 mg/L BA without 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 mg/L 2,4-D and 0.5-1.0 mg/L 6-BA, respectively. Cyto-morphological and histological changes in the shoot development via calli and GGBs were examined...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28492516/circulating-cell-free-dna-and-circulating-tumor-cells-as-prognostic-and-predictive-biomarkers-in-advanced-non-small-cell-lung-cancer-patients-treated-with-first-line-chemotherapy
#6
Simona Coco, Angela Alama, Irene Vanni, Vincenzo Fontana, Carlo Genova, Maria Giovanna Dal Bello, Anna Truini, Erika Rijavec, Federica Biello, Claudio Sini, Giovanni Burrafato, Claudia Maggioni, Giulia Barletta, Francesco Grossi
Cell-free DNA (cfDNA) and circulating tumor cells (CTCs) are promising prognostic and predictive biomarkers in non-small cell lung cancer (NSCLC). In this study, we examined the prognostic role of cfDNA and CTCs, in separate and joint analyses, in NSCLC patients receiving first line chemotherapy. Seventy-three patients with advanced NSCLC were enrolled in this study. CfDNA and CTC were analyzed at baseline and after two cycles of chemotherapy. Plasma cfDNA quantification was performed by quantitative PCR (qPCR) whereas CTCs were isolated by the ScreenCell Cyto (ScreenCell, Paris, France) device and enumerated according to malignant features...
May 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28491095/angelica-sinensis-polysaccharide-prevents-hematopoietic-stem-cells-senescence-in-d-galactose-induced-aging-mouse-model
#7
Xinyi Mu, Yanyan Zhang, Jing Li, Jieyu Xia, Xiongbin Chen, Pengwei Jing, Xiaoying Song, Lu Wang, Yaping Wang
Age-related regression in hematopoietic stem/progenitor cells (HSC/HPCs) limits replenishment of the blood and immune system and hence contributes to hematopoietic diseases and declined immunity. In this study, we employed D-gal-induced aging mouse model and observed the antiaging effects of Angelica Sinensis Polysaccharide (ASP), a major active ingredient in dong quai (Chinese Angelica Sinensis), on the Sca-1(+) HSC/HPCs in vivo. ASP treatment prevents HSC/HPCs senescence with decreased AGEs levels in the serum, reduced SA-β-Gal positive cells, and promoted CFU-Mix formation in the D-gal administrated mouse...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28488794/apoptotic-activity-of-lactobacillus-plantarum-dgk-17-fermented-soybean-seed-extract-in-human-colon-cancer-cells-via-ros-jnk-signaling-pathway
#8
Imran Khan, Sun Chul Kang
Fermented food has been always possesses upper hand compared to normal food due to its antibacterial, antioxidant, and anticancer properties. Soybeans, which have high nutritional value, are widely consumed in Korea. In this study, soybean seed powder fermented with Lactobacillus plantarum DGK-17, which was previously isolated from kimchi, showed anticancer potential. Fermented soybean extract (FSE) resulted in morphological changes, reduction of cancer cell colony formation and apoptotic cell death of HCT-116 colon cancer cells in a dose-dependent manner, and IC50 value of 111 μg...
May 10, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28480386/pre-clinical-evaluation-of-extracts-and-essential-oils-from-betel-like-scent-piper-species-identified-potential-cancer-treatment
#9
Arisa Sanubol, Arunrat Chaveerach, Tawatchai Tanee, Runglawan Sudmoon
BACKGROUND: Nine Piper species with betel-like scents are sources of industrial and medicinal aromatic chemicals, but there is lack of information on cytotoxicity and genotoxicity for human safety, including how these plants impact human cervical cancer cell line. METHODS: Plant leaves were extracted with hexane and hydro-distilled for essential oils. The extracts and oils were pre-clinically studied based on cyto - and genotoxicity using microculture tetrazolium (MTT) and comet assays...
2017: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
https://www.readbyqxmd.com/read/28473753/mechanical-strain-promotes-oligodendrocyte-differentiation-by-global-changes-of-gene-expression
#10
Anna Jagielska, Alexis L Lowe, Ekta Makhija, Liliana Wroblewska, Jochen Guck, Robin J M Franklin, G V Shivashankar, Krystyn J Van Vliet
Differentiation of oligodendrocyte progenitor cells (OPC) to oligodendrocytes and subsequent axon myelination are critical steps in vertebrate central nervous system (CNS) development and regeneration. Growing evidence supports the significance of mechanical factors in oligodendrocyte biology. Here, we explore the effect of mechanical strains within physiological range on OPC proliferation and differentiation, and strain-associated changes in chromatin structure, epigenetics, and gene expression. Sustained tensile strain of 10-15% inhibited OPC proliferation and promoted differentiation into oligodendrocytes...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28467694/durable-control-of-autoimmune-diabetes-in-mice-achieved-by-intraperitoneal-transplantation-of-neo-islets-three-dimensional-aggregates-of-allogeneic-islet-and-mesenchymal-stem-cells
#11
Christof Westenfelder, Anna Gooch, Zhuma Hu, Jon Ahlstrom, Ping Zhang
Novel interventions that reestablish endogenous insulin secretion and thereby halt progressive end-organ damage and prolong survival of patients with autoimmune Type 1 diabetes mellitus (T1DM) are urgently needed. While this is currently accomplished with allogeneic pancreas or islet transplants, their utility is significantly limited by both the scarcity of organ donors and life-long need for often-toxic antirejection drugs. Coadministering islets with bone marrow-derived mesenchymal stem cells (MSCs) that exert robust immune-modulating, anti-inflammatory, anti-apoptotic, and angiogenic actions, improves intrahepatic islet survival and function...
May 3, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28464994/genotoxic-effects-of-zinc-oxide-nanoparticles-in-nasal-mucosa-cells-are-antagonized-by-titanium-dioxide-nanoparticles
#12
Stephan Hackenberg, Agmal Scherzed, Angela Zapp, Katrin Radeloff, Christian Ginzkey, Thomas Gehrke, Pascal Ickrath, Norbert Kleinsasser
Titanium dioxide nanoparticles (TiO2-NPs) and zinc oxide nanoparticles (ZnO-NPs) are often used in sunscreens and other consumer products due to their photoprotective properties. However, concern exists regarding them possibly causing cyto- and genotoxic effects. The aim of this study was to assess cyto- and genotoxicity of these nanomaterials after single or combined exposure. For this purpose, a battery of cell culture test systems for human nasal mucosa (monolayer, air-liquid interface and mini organ culture) were exposed to 0...
April 2017: Mutation Research
https://www.readbyqxmd.com/read/28451989/tissue-engineered-human-amniotic-membrane-application-in-mouse-ovarian-follicular-culture
#13
M Motamed, Z Sadr, M R Valojerdi, A Moini, S Oryan, M Totonchi, B Ebrahimi, S Maroufizadeh, E Taghiabadi, R Fathi
Since folliculogenesis requires a powerful cell-matrix interaction, natural scaffolds seem to be needed for follicular culture. Human amniotic membrane (HAM) offers promise as a support of in vitro ovarian follicular culture. HAM was decellularized with trypsin and EDTA. DNA and histology assays were performed to determine the elimination rate of genomic components. Cyto-biocompatibility of decellular AM (DAM) was verified by the cell viability (MTT) test. The small parts of intact amniotic membrane (IAM) and DAM were coated on the bottom of 96-well and each well was filled with 150 µL of base medium...
April 27, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28451739/understanding-renal-nuclear-protein-accumulation-an-in-vitro-approach-to-explain-an-in-vivo-phenomenon
#14
Lisanne Luks, Marcia Y Maier, Silvia Sacchi, Loredano Pollegioni, Daniel R Dietrich
Proper subcellular trafficking is essential to prevent protein mislocalization and aggregation. Transport of the peroxisomal enzyme D-amino acid oxidase (DAAO) appears dysregulated by specific pharmaceuticals, e.g., the anti-overactive bladder drug propiverine or a norepinephrine/serotonin reuptake inhibitor (NSRI), resulting in massive cytosolic and nuclear accumulations in rat kidney. To assess the underlying molecular mechanism of the latter, we aimed to characterize the nature of peroxisomal and cyto-nuclear shuttling of human and rat DAAO overexpressed in three cell lines using confocal microscopy...
April 27, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28450001/optimisation-of-pueraria-isoflavonoids-by-response-surface-methodology-using-ultrasonic-assisted-extraction
#15
Ka H Wong, George Q Li, Kong M Li, Valentina Razmovski-Naumovski, Kelvin Chan
Puerariae Lobatae Radix (PLR) exerts cyto-protective effect against oxidative stress due to its high isoflavonoid content. In this study, the ultrasonic-assisted extraction condition for the maximum recovery of isoflavonoids with high cyto-protective effect was optimised by response surface methodology (RSM). A second-order polynomial fitted the experimental data (R(2): 0.9736; p-value <0.0001). The optimal extraction parameters were determined as: extraction time 16.02min, ethanol concentration 41.41% and liquid-to-solid ratio 44...
September 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28449966/environmental-concentrations-of-cocaine-and-its-main-metabolites-modulated-antioxidant-response-and-caused-cyto-genotoxic-effects-in-zebrafish-embryo-cells
#16
Marco Parolini, Anna Ghilardi, Camilla Della Torre, Stefano Magni, Laura Prosperi, Matteo Calvagno, Luca Del Giacco, Andrea Binelli
Illicit drugs have been recently identified as a serious environmental problem because of the growing evidence regarding their occurrence in aquatic environment and potential toxicity towards non-target organisms. Among them, cocaine (COC) and its main metabolites, namely benzoylecgonine (BE) and ecgonine methyl ester (EME), are commonly measured in freshwaters worldwide at levels that might cause diverse sub-lethal effects to aquatic organisms. Thus, the present study was aimed at investigating the potential adverse effects induced by the exposure to environmental concentrations (0...
April 24, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28449660/selective-measurement-of-%C3%AE-smooth-muscle-actin-why-%C3%AE-actin-can-not-be-used-as-a-housekeeping-gene-when-tissue-fibrosis-occurs
#17
Apor Veres-Székely, Domonkos Pap, Erna Sziksz, Eszter Jávorszky, Réka Rokonay, Rita Lippai, Kálmán Tory, Andrea Fekete, Tivadar Tulassay, Attila J Szabó, Ádám Vannay
BACKGROUND: Prevalence of fibroproliferative diseases, including chronic kidney disease is rapidly increasing and has become a major public health problem worldwide. Fibroproliferative diseases are characterized by increased expression of α smooth muscle actin (α-SMA) that belongs to the family of the six conserved actin isoforms showing high degree homology. The aim of the present study was to develop real-time PCRs that clearly discriminate α-SMA and ß-actin from other actin isoforms...
April 27, 2017: BMC Molecular Biology
https://www.readbyqxmd.com/read/28449183/noninferiority-trial-investigating-the-efficacy-of-a-nonantibiotic-intramammary-therapy-in-the-treatment-of-mild-to-moderate-clinical-mastitis-in-dairy-cows-with-longer-lasting-udder-diseases
#18
M Ziesch, N Wente, Y Zhang, W Zaremba, S Engl, V Krömker
A nonblinded, positively controlled, noninferiority trial was conducted to evaluate the efficacy of an alternative, nonantibiotic therapy with Masti Veyxym(®) to reduce ineffective antibiotic usage in the treatment of nonsevere clinical mastitis (CM) in cows with longer lasting udder diseases. The solely intramammary treatment with Masti Veyxym(®) (three applications, 12 hr apart) and the combined treatment with Masti Veyxym(®) and antibiotics as usual on the farm according to label of the respective product were compared with the reference treatment of solely antibiotic therapy...
April 27, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28442922/inhibition-of-pcsk9-protects-against-radiation-induced-damage-of-prostate-cancer-cells
#19
Si-Shun Gan, Jian-Qing Ye, Lei Wang, Fa-Jun Qu, Chuan-Min Chu, Yi-Jun Tian, Wei Yang, Xin-Gang Cui
BACKGROUND: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a protein expressed primarily in the liver, formerly known to maintain plasma lipid homeostasis by regulating low-density lipoprotein receptor levels, and its exact role in the radioresistance of prostate cancer (PCa) remains unclear. We aim to investigate the function of PCSK9 in the radioresistance of PCa cells. METHODS: PCSK9 small interfering RNA (siRNA) was introduced into the PCa cells by transient transfection...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28439892/cerebrospinal-fluid-cyto-chemokine-profile-during-acute-herpes-simplex-virus-induced-anti-n-methyl-d-aspartate-receptor-encephalitis-and-in-chronic-neurological-sequelae
#20
Kavitha Kothur, Deepak Gill, Melanie Wong, Shekeeb S Mohammad, Sushil Bandodkar, Susan Arbunckle, Louise Wienholt, Russell C Dale
AIM: To examine the cytokine/chemokine profile of cerebrospinal fluid (CSF) during acute herpes simplex virus-induced N-methyl-d-aspartate receptor (NMDAR) autoimmunity and in chronic/relapsing post-herpes simplex virus encephalitis (HSE) neurological syndromes. METHOD: We measured longitudinal serial CSF cyto-/chemokines (n=34) and a glial marker (calcium-binding astroglial protein, S100B) in one patient during acute HSE and subsequent anti-NMDAR encephalitis, and compared the results with those from two patients with anti-NMDAR encephalitis without preceding HSE...
April 25, 2017: Developmental Medicine and Child Neurology
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