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https://www.readbyqxmd.com/read/29677633/differences-in-the-bovine-milk-whey-proteome-between-early-pregnancy-and-the-estrous-cycle
#1
D Johnston, I Malo Estepa, H A Ebhardt, M A Crowe, M G Diskin
Current bovine pregnancy detection methods are not reliable until at least day 28 post artificial insemination (AI). The bovine estrous cycle is approximately 21 days; consequently, producers miss an opportunity to rebreed at the next estrous event. Therefore, commercial interest exists for the discovery of novel biomarkers of pregnancy which could reliably detect pregnancy status at or before day 21 of pregnancy. The objective of the present study was to use liquid chromatography tandem mass spectrometry (LC-MS/MS) to perform a global, label-free, proteomics study on (i) milk whey and (ii) extracellular vesicle (EV) enriched milk whey samples, from day 21 of pregnancy, compared with day 21 of the estrous cycle, in order to identify potential protein biomarkers of early pregnancy...
April 12, 2018: Theriogenology
https://www.readbyqxmd.com/read/29675640/effects-of-combined-whole-body-electromyostimulation-and-protein-supplementation-on-local-and-overall-muscle-fat-distribution-in-older-men-with-sarcopenic-obesity-the-randomized-controlled-franconia-sarcopenic-obesity-franso-study
#2
Wolfgang Kemmler, Alexandra Grimm, Michael Bebenek, Matthias Kohl, Simon von Stengel
The primary aim of the project was to determine the combined effect of whole-body electromyostimulation (WB-EMS) and protein supplements on local and overall muscle/fat distribution in older man with sarcopenic obesity (SO). Community-dwelling (cdw) men ≥ 70 years with SO were randomly allocated to a WB-EMS and protein supplementation (n = 33) or a non-intervention control group (CG: n = 34). WB-EMS was conducted 1.5 sessions of 20 min/week for 16 weeks. Whey protein supplementation aimed to ensure a daily intake of 1...
April 19, 2018: Calcified Tissue International
https://www.readbyqxmd.com/read/29674266/effects-of-high-protein-diet-containing-isolated-whey-protein-in-rats-submitted-to-resistance-training-of-aquatic-jumps
#3
Eudes Thiago Pereira Avila, Thiago da Rosa Lima, Ramires Alsamir Tibana, Paula Caroline de Almeida, Géssica Alves Fraga, Mariana de Souza Sena, Luiz Felipe Petusk Corona, James Wilfred Navalta, Sajjad Rezaei, Morteza Ghayomzadeh, Amílcar Sabino Damazo, Jonato Prestes, Fabrício Azevedo Voltarelli
OBJECTIVES: Isolated whey protein (IWP) can decrease body fat compared with other protein sources. The present study verified the effects of high protein diet (HD) containing IWP on several parameters of rats subjected to resistance training (RT). METHODS: Thirty-two male Wistar rats (60 days of age) were separated into four groups (n = 8/group): sedentary normoproteic (IWP 14%; SN); sedentary hyperproteic (IWP 35%; SH); trained normoproteic (IWP 14%; TN), and trained hyperproteic (WPI 35%; TH)...
February 13, 2018: Nutrition
https://www.readbyqxmd.com/read/29674146/chlorella-vulgaris-mixotrophic-growth-enhanced-biomass-productivity-and-reduced-toxicity-from-agro-industrial-by-products
#4
Rebeca Gonçalves de Melo, Alexsandra Frazão de Andrade, Raquel Pedrosa Bezerra, Dominick Spindola Correia, Vanessa Cristina de Souza, Ana Christina Brasileiro-Vidal, Daniela de Araújo Viana Marques, Ana Lúcia Figueiredo Porto
Algal wastewater remediation has become attractive for a couple of years now, however the effectiveness of genetic toxicity reducing of some by-products through microalgae are still not well reported. This study aimed to evaluate the growth, nutrients and toxicity removal of Chlorella vulgaris cultivated under autotrophic and mixotrophic conditions in three agro-industrial by-products. Mixotrophic culture using corn steep liquor showed higher cell concentration, specific growth rate, maximum cell productivity and biomass protein content when compared to cheese whey and vinasse...
April 16, 2018: Chemosphere
https://www.readbyqxmd.com/read/29671767/effects-of-whey-protein-hydrolysate-ingestion-on-postprandial-aminoacidemia-compared-with-a-free-amino-acid-mixture-in-young-men
#5
Kyosuke Nakayama, Chiaki Sanbongi, Shuji Ikegami
To stimulate muscle protein synthesis, it is important to increase the plasma levels of essential amino acids (EAA), especially leucine, by ingesting proteins. Protein hydrolysate ingestion can induce postprandial hyperaminoacidemia; however, it is unclear whether protein hydrolysate is associated with higher levels of aminoacidemia compared with a free amino acid mixture when both are ingested orally. We assessed the effects of whey protein hydrolysate (WPH) ingestion on postprandial aminoacidemia, especially plasma leucine levels, compared to ingestion of a free amino acid mixture...
April 19, 2018: Nutrients
https://www.readbyqxmd.com/read/29670601/population-dynamics-of-lactobacillus-helveticus-in-swiss-gruy%C3%A3-re-type-cheese-manufactured-with-natural-whey-cultures
#6
Aline Moser, Karl Schafroth, Leo Meile, Lotti Egger, René Badertscher, Stefan Irmler
Lactobacillus helveticus , a ubiquitous bacterial species in natural whey cultures (NWCs) used for Swiss Gruyère cheese production, is considered to have crucial functions for cheese ripening such as enhancing proteolysis. We tracked the diversity and abundance of L. helveticus strains during 6 months of ripening in eight Swiss Gruyère-type cheeses using a culture-independent typing method. The study showed that the L. helveticus population present in NWCs persisted in cheese and demonstrated a stable multi-strain coexistence during cheese ripening...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29663394/polymorphism-in-ovine-anxa9-gene-and-physic-chemical-properties-and-the-fraction-of-protein-in-milk
#7
Ewa Pecka-Kiełb, Ewa Czerniawska-Piątkowska, Inga Kowalewska-Łuczak, Milan Vasil
BACKGROUND: Annexin A9 (ANXA9) is a specific fatty acid transport protein. ANXA9 gene is expressed in various tissues, including secretory tissue and mammary glands. The association between three SNPs of the ANXA9 gene and sheep's milk compositions was assessed. RESULTS: Genotype analysis was performed with the use of PCR-RFLP method. The studied ANXA9 polymorphisms had the following MAF (Major Allele Frequency): SNP1: allele G 0,66; SNP2: allele G 0,54; SNP3: allele C 0,57...
April 16, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29663020/the-self-association-and-thermal-denaturation-of-caprine-and-bovine-%C3%AE-lactoglobulin
#8
Jennifer M Crowther, Jane R Allison, Grant A Smolenski, Alison J Hodgkinson, Geoffrey B Jameson, Renwick C J Dobson
Milk components, such as proteins and lipids, have different physicochemical properties depending upon the mammalian species from which they come. Understanding the different responses of these milks to digestion, processing, and differences in their immunogenicity requires detailed knowledge of these physicochemical properties. Here we report on the oligomeric state of β-lactoglobulin from caprine milk, the most abundant protein present in the whey fraction. At pH 2.5 caprine β-lactoglobulin is predominantly monomeric, whereas bovine β-lactoglobulin exists in a monomer-dimer equilibrium at the same protein concentrations...
April 16, 2018: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/29660720/environmental-impact-of-cheese-production-a-case-study-of-a-small-scale-factory-in-southern-europe-and-global-overview-of-carbon-footprint
#9
Fernando Canellada, Amanda Laca, Adriana Laca, Mario Díaz
The environmental performance of a small-scale cheese factory sited in a NW Spanish region has been analysed by Life Cycle Assessment (LCA) as representative of numerous cheese traditional factories that are scattered through the European Union, especially in the southern countries. Inventory data were directly obtained from this facility corresponding to one-year operation, and the main subsystems involved in cheese production were included, i.e. raw materials, water, electricity, energy, cleaning products, packaging materials, transports, solid and liquid wastes and gas emissions...
April 13, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29650043/experimental-studies-on-effects-of-diet-on-lawsonia-intracellularis-infections-in-fattening-boars-in-a-natural-infection-model
#10
Christian Visscher, Anne Kruse, Saara Sander, Christoph Keller, Jasmin Mischok, Robert Tabeling, Hubert Henne, Ricarda Deitmer, Josef Kamphues
BACKGROUND: Lawsonia intracellularis is one of the most economically important pathogens in swine production. This study tested the hypothesis that the composition of diets for pigs has an impact on the excretion of L. intracellularis in a natural infection model. RESULTS: Fifty boars (~ 90 kg BW) from a SPF-farm with a strict hygiene and management regime for reducing the spread of an L. intracellularis infection up to the beginning of the final fattening period were transported, regrouped and randomly allotted to groups of five animals each at the research facility...
April 12, 2018: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/29648971/anti-inflammatory-effects-of-a-mixture-of-lactic-acid-bacteria-and-sodium-butyrate-in-atopic-dermatitis-murine-model
#11
Jeong A Kim, Sung-Hak Kim, In Sung Kim, Da Yoon Yu, Sung Chan Kim, Seung Ho Lee, Sang Suk Lee, Cheol-Heui Yun, In Soon Choi, Kwang Keun Cho
Atopic dermatitis is a chronic and recurrent inflammatory skin disease. Recently, probiotics have been shown to suppress allergic symptoms through immunomodulatory responses. In the present study, combinatorial effects on allergic symptoms were identified in BALB/c mice fed with a mixture of four species of probiotics, Bifidobacterium lactis, Lactobacillus casei, Lactobacillus rhamnosus, and Lactobacillus plantarum, and sodium butyrate. Following sensitization with whey protein, the mice were challenged and divided into two groups: (1) mice administered with phosphate-buffered saline as a control and (2) mice administered with the probiotic mixture and sodium butyrate...
March 20, 2018: Journal of Medicinal Food
https://www.readbyqxmd.com/read/29648824/impact-of-interfacial-composition-on-lipid-and-protein-co-oxidation-in-oil-in-water-emulsions-containing-mixed-emulisifers
#12
Zhenbao Zhu, Cui Zhao, Jianhua Yi, Ning Liu, Yungang Cao, Eric A Decker, David Julian McClements
The impact of interfacial composition on lipid and protein co-oxidation in oil-in-water emulsions containing a mixture of proteins and surfactants was investigated. The emulsions consisted of 5% v/v walnut oil, 0.5% w/v whey protein isolate (WPI), and 0 to 0.4% w/v Tween 20 (pH 3 and 7). The protein surface load, magnitude of the ξ-potential, and mean particle diameter of the emulsions decreased as the Tween 20 concentration was increased, indicating the whey proteins were displaced by this non-ionic surfactant...
April 12, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29644961/high-intensity-exercise-induced-intestinal-damage-is-protected-by-fermented-milk-supplemented-with-whey-protein-probiotic-and-pomegranate-punica-granatum-l
#13
Fernanda M Chaves, Igor L Baptista, Fernando M Simabuco, Paula G F Quaresma, Fabiola L Pena, Rosangela M N Bezerra, Jose R Pauli, Diogo T da Cunha, Patricia L Campos-Ferraz, Adriane E C Antunes
Here we evaluated the effect of fermented milk supplemented with whey protein (approximately 80 % protein), probiotic (Bifidobacterium animalis subsp. lactis BB12) and pomegranate juice (Punica granatum L.) on the physical performance, intestinal motility and villi structure, inflammatory markers and intestinal microbiota of rats under high-intensity acute exercise. In all, twenty-four Wistar rats were separated into groups: control (Ctrl), supplemented (Supp), exercised (Exe) and exercised and supplemented (Exe+Supp)...
April 2018: British Journal of Nutrition
https://www.readbyqxmd.com/read/29627243/effect-of-milk-centrifugation-and-incorporation-of-high-heat-treated-centrifugate-on-the-composition-texture-and-ripening-characteristics-of-maasdam-cheese
#14
Prabin Lamichhane, Alan L Kelly, Jeremiah J Sheehan
This study investigated the effect of centrifugation (9,000 × g, 50°C, flow rate = 1,000 L/h), as well as the incorporation of high-heat-treated (HHT) centrifugate into cheese milk on the composition, texture, and ripening characteristics of Maasdam cheese. Neither centrifugation nor incorporation of HHT centrifugate into cheese milk had a pronounced effect on the compositional parameters of any experimental cheeses, except for moisture and moisture in nonfat substance (MNFS) levels. Incorporation of HHT centrifugate at a rate of 6 to 10% of the total milk weight into centrifuged milk increased the level of denatured whey protein in the cheese milk and also increased the level of MNFS in the resultant cheese compared with cheeses made from centrifuged milk and control cheeses; moreover, cheese made from centrifuged milk had ∼3% higher moisture content on average than control cheeses...
April 4, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29626232/a-pre-meal-of-whey-proteins-induces-differential-effects-on-glucose-and-lipid-metabolism-in-subjects-with-the-metabolic-syndrome-a-randomised-cross-over-trial
#15
Ann Bjørnshave, Jens Juul Holst, Kjeld Hermansen
PURPOSE: Postprandial lipaemia (PPL), an independent risk factor for cardiovascular disease, is affected by composition and timing of meals. We evaluated if whey proteins (WP) consumed as a pre-meal before a fat-rich meal reduce postprandial triglyceride (TG) and apolipoprotein B-48 (ApoB-48) responses in subjects with the metabolic syndrome (MeS). METHODS: An acute, randomised, cross-over trial was conducted. 20 subjects with MeS consumed a pre-meal of 0, 10 or 20 g WP 15 min prior to a fat-rich meal...
April 6, 2018: European Journal of Nutrition
https://www.readbyqxmd.com/read/29623688/dietary-approaches-reducing-boar-taint-importance-of-lawsonia-intracellularis-colonisation-for-interpreting-results
#16
C Visscher, A Kruse, S Sander, C Keller, J Mischok, R Tabeling, H Henne, R Deitmer, J Kamphues
In the fattening of male pigs, boar odour is a major problem with regard to the acceptance of the meat by consumers. Skatole can be one cause. Tryptophan from non-digested feed ingredients and intestinal cell debris can be the precursor in skatole formation. Lawsonia intracellularis, one of the most widespread pathogens in swine, promotes the epithelial cell turnover and might favour the tryptophan influx into the hindgut. Therefore, the question arises how far the severity of a Lawsonia intracellularis infection has an effect on results of dietary experiments with specific issues...
April 2018: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29623686/effect-of-a-montmorillonite-bentonite-based-product-on-faecal-parameters-of-horses
#17
K Gerstner, A Liesegang
Free faecal water (fluid excreted separate from normal faeces) is a common problem of unknown origin in horses. Multifactorial causes are assumed. This study assessed the effect of a commercial product (EMP) sold to help support intestinal health of horses, particularly regarding the occurrence of free faecal water. The hypothesis was that water-binding capacity and microbial activity are improved in the large intestine of horses fed EMP. EMP contained montmorillonite-bentonite, whey and extracts from hop and absinthium...
April 2018: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29623575/effect-of-eribulin-on-patients-with-metastatic-breast-cancer-multicenter-retrospective-observational-study-in-taiwan
#18
Kun-Ming Rau, Fu Ou-Yang, Ta-Chung Chao, Yao-Lung Kuo, Tsui-Fen Cheng, Tsu-Yi Chao, Dar-Ren Chen, Yen-Dun Tzeng, Being-Whey Wang, Chun-Yu Liu, Ming-Hung Hu, Yin-Che Lu, Wei-Jen Ou, Chin-Ho Kuo, Chieh-Han Chuang, Jung-Yu Kan, Fang-Ming Chen, Ming-Feng Hou
PURPOSE: The aim of this study was to confirm the therapeutic role of eribulin on Taiwanese women with metastatic breast cancer. METHODS: This retrospective study examined 449 females who received eribulin between March 2014 and June 2017 at 14 hospitals in Taiwan for treatment of locally advanced or metastatic breast cancer. RESULTS: The survival rate at 24 months was 57.2% (95% CI 51.0-62.9%) and the median time to treatment failure (TTF) was 3...
April 5, 2018: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/29622223/characterisation-of-%C3%AE-carotene-partitioning-in-protein-emulsions-effects-of-pre-treatments-solid-fat-content-and-emulsifier-type
#19
W A Fahmi Wan Mohamad, Donald McNaughton, Mary Ann Augustin, Roman Buckow
Understanding the bioactive partitioning between the phases of an emulsion system underpins strategies for improving the efficiency of bioactive protection against degradation. We analysed partitioning of β-carotene in emulsions with various formulations in-situ using confocal Raman microscopy (CRM). The partitioning of β-carotene into the aqueous phase of emulsions increased when whey protein isolate (WPI) was heat or high pressure-treated prior to emulsion formation. However, increasing the concentration of high pressure-treated WPI reduced the β-carotene partitioning into the aqueous phase...
August 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29622211/characterization-and-comparison-of-the-structure-and-antioxidant-activity-of-glycosylated-whey-peptides-from-two-pathways
#20
Xinliu Zhang, Hao Gao, Chunyan Wang, Abdul Qayum, Zhishen Mu, Zengli Gao, Zhanmei Jiang
Glycosylated whey peptides (WPP-Gal A and WPP-Gal B) were formed from two pathways. WPP-Gal A showed higher browning intensity and fluorescence intensity than WPP-Gal B. Loss of amino groups and reducing sugar was heavier for WPP-Gal A than WPP-Gal B. Size exclusion chromatography revealed that WPP-Gal A and B had similar curves, but the molecular weight distribution of WPP-Gal B was more extensive than that of WPP-Gal A. Particle size was larger for WPP-Gal B than WPP-Gal A. Furthermore, WPP-Gal A showed greater antioxidant activity than WPP-Gal B...
August 15, 2018: Food Chemistry
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