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https://www.readbyqxmd.com/read/29056672/immuno-detection-of-c3a-a-c3-complement-activated-product-in-mastitis-milk-a-potential-diagnostic-marker
#1
Thanislass Jacob, Gangasudan Subramani, Prathiba Sivaprakasam, Antony P Xavier, Hirak K Mukhopadhyay
The sub-clinical form of mastitis is difficult to detect and causes huge economic loss to the dairy industry. It has become a threat to public health at large, thus there is a need for definite diagnosis of the disease. Therefore, this study was undertaken to identify the novel diagnostic marker for the detection of the sub-clinical form of mastitis. Two-dimensional gel analysis of the whey protein fraction of normal and mastitis milk samples revealed the presence of proteose peptone component 3 precursor, Trypsin precursor, complement component-C3, Ig heavy chain precursors and a C-type lectin domain as differentially expressed protein during the early stage of mastitis...
February 23, 2017: Veterinary Sciences
https://www.readbyqxmd.com/read/29055974/technology-development-of-protein-rich-concentrates-for-nutrition-in-extreme-conditions-using-soybean-and-meat-by-products
#2
Tatiana K Kalenik, Rui Costa, Elena V Motkina, Tamara A Kosenko, Olga V Skripko, Irina A Kadnikova
BACKGROUND: There is a need to develop new foods for participants of expeditions in extreme conditions, which must be self-sufficient. These foods should be light to carry, with a long shelf life, tasty and with  high nutrient density. Currently, protein sources are limited mainly to dried and canned meat. In this work, a protein-rich dried concentrate suitable for extreme expeditions was developed using soya, tomato, milk whey and meat by-products. METHODS: Protein concentrates were developed using minced beef liver and heart, dehydrated and mixed with a soya protein-lycopene coagulate (SPLC) obtained from a solution prepared with germi- nated soybeans and mixed with tomato paste in milk whey, and finally dried...
July 2017: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/29054827/characterization-of-a-double-wap-domain-containing-protein-from-the-red-swamp-crayfish-procambarus-clarkii
#3
Hong-Wei Zhang, Xin Man, Yue Wang, Qi-Sheng Song, David Stanley, Kai-Min Hui, Xiao-Wen Zhang
Crustaceans express multiple whey acidic protein (WAP) domain containing proteins which are components of host immunity. In the present study, a new double WAP domain containing protein was identified from red swamp crayfish Procambarus clarkii, designated Pc-DWD. The ORF is 387 bp, encoding 128 amino acids consisting of signal peptide of 18 residues, and two tandem WAP domains of 38 and 44 residues. Multiple alignment indicates the presence of conserved motifs in both WAP domains, and phylogenetic analysis shows that Pc-DWD is a new member of the type-IV crustin family...
October 17, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29053995/effects-of-a-whey-protein-supplementation-on-oxidative-stress-body-composition-and-glucose-metabolism-among-overweight-people-affected-by-diabetes-mellitus-or-impaired-fasting-glucose-a-pilot-study
#4
Chiara Flaim, Michael Kob, Angela M Di Pierro, Markus Herrmann, Lucio Lucchin
Obesity and diabetes mellitus type 2 (DM2) are characterized by chronic inflammation and oxidative stress [Donath et al. 2013] and this leads to cardiovascular diseases [Hulsmans & Holvoet 2010]. Whey proteins (WP) have antioxidant [Chitapanarux et al. 2009], anti-inflammatory [Sugawara et al. 2012] and hypoglycemic activities [Mignone et al. 2015], while data on weight, body composition [Frestedt et al. 2008; Aldrich et al. 2011] and blood pressure are conflicting [Kawase et al. 2000; Lee et al. 2007]. WP have unpleasant taste and smell [Patel 2015], but a new WP isolate (ProLYOtin®) seems to be more palatable...
May 20, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29051646/effect-of-fiber-blends-total-solids-heat-treatment-whey-protein-concentrate-and-stage-of-sugar-incorporation-on-dietary-fiber-fortified-kheer
#5
Simran Kaur Arora, A A Patel
Owing to the proven beneficial role of dietary fiber (DF) on human health, feasibility of incorporating commercially available soluble and insoluble DF preparations into rice-milk-pudding (kheer, a popular Indian delicacy) was studied through process modification. The novel approach of preparing reduced fat DF-fortified-kheer (DFFK) by developing liquid/cream phase and particulate/rice phase separately, and subsequently blending the two was developed. The major processing variables studied were total solids (TS) in the liquid phase, type of fiber blend, flavor-simulation through heat treatment or added whey protein, and the presence of sugar in water for pre-cooking of rice...
October 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29045496/at-same-leucine-intake-a-whey-plant-protein-blend-is-not-as-effective-as-whey-to-initiate-a-transient-post-prandial-muscle-anabolic-response-during-a-catabolic-state-in-mini-pigs
#6
Aurélia Revel, Marianne Jarzaguet, Marie-Agnès Peyron, Isabelle Papet, Noureddine Hafnaoui, Carole Migné, Laurent Mosoni, Sergio Polakof, Isabelle Savary-Auzeloux, Didier Rémond, Dominique Dardevet
BACKGROUND: Muscle atrophy has been explained by an anabolic resistance following food intake and an increase of dietary protein intake is recommended. To be optimal, a dietary protein has to be effective not only to initiate but also to prolong a muscle anabolic response in a catabolic state. To our knowledge, whether or not a dairy or a dairy/plant protein blend fulfills these criterions is unknown in a muscle wasting situation. OBJECTIVE: Our aim was, in a control and a catabolic state, to measure continuously muscle anabolism in term of intensity and duration in response to a meal containing casein (CAS), whey (WHEY) or a whey/ plant protein blend (BLEND) and to evaluate the best protein source to elicit the best post prandial anabolism according to the physio-pathological state...
2017: PloS One
https://www.readbyqxmd.com/read/29045254/nutritional-interventions-in-sarcopenia-where-do-we-stand
#7
Jean Woo
PURPOSE OF REVIEW: To document recent evidence regarding the role of nutrition as an intervention for sarcopenia. RECENT FINDINGS: A review of seven randomized controlled trials (RCTs) on beta-hydroxy-beta-methylbutyrate (HMB) alone on muscle loss in 147 adults showed greater muscle mass gain in the intervention group, but no benefit in muscle strength and physical performance measures. Three other review articles examined nutrition and exercise as combined intervention, and suggest enhancement of benefits of exercise by nutrition supplements (energy, protein, vitamin D)...
October 17, 2017: Current Opinion in Clinical Nutrition and Metabolic Care
https://www.readbyqxmd.com/read/29042603/separation-of-antioxidant-peptides-from-pepsin-hydrolysate-of-whey-protein-isolate-by-atps-of-eopo-co-polymer-ucon-phosphate
#8
Bin Jiang, Xiaoqing Zhang, Yongqiang Yuan, Yuxiao Qu, Zhibiao Feng
An aqueous two-phase system (ATPS) consisting of poly(ethylene glycol-ran-propylene glycol) monobutyl ether (UCON)/phosphate was developed for the separation of the antioxidant peptides from pepsin hydrolysate of Whey Protein Isolate (WPI). The efficiency of the separation was evaluated based on the DPPH radical scavenging activity, ABTS radical scavenging activity and ferric reducing antioxidant power (FRAP) of the separated peptides. The effects of some parameters on the partition of antioxidant peptides were investigated...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29042449/peptide-yy-mediates-the-satiety-effects-of-diets-enriched-with-whey-protein-fractions-in-male-rats
#9
Rizaldy C Zapata, Arashdeep Singh, Prasanth K Chelikani
Dairy proteins-whey protein, in particular-are satiating and often recommended for weight control; however, little is known about the mechanisms by which whey protein and its components promote satiety and weight loss. We used diet-induced obese rats to determine whether the hypophagic effects of diets that are enriched with whey and its fractions, lactalbumin and lactoferrin, are mediated by the gut hormone, peptide YY (PYY). We demonstrate that high protein diets that contain whey, lactalbumin, and lactoferrin decreased food intake and body weight with a concurrent increase in PYY mRNA abundance in the colon and/or plasma PYY concentrations...
October 17, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29040857/synergistic-effects-of-anaerobic-co-digestion-of-whey-manure-and-fish-ensilage
#10
Vivekanand Vivekanand, Daniel Girma Mulat, Vincent G H Eijsink, Svein J Horn
Biogas production potential of the three feedstocks fish ensilage, manure and whey was evaluated using biochemical methane potential (BMP) tests. Since anaerobic digestion of single substrates may be inefficient due to imbalances in the carbon-nitrogen ratio, degree of biodegradability and/or due to lack of nutrients needed by the microbial community, co-digestion of these substrates was also assessed, revealing synergistic effects and a particularly good effect of combining manure with fish ensilage. In this latter case, methane yields were up to 84% higher than the weighted average of the methane yields obtained with the individual substrates...
September 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29037706/lutein-enriched-emulsion-based-delivery-systems-influence-of-emulsifiers-and-antioxidants-on-physical-and-chemical-stability
#11
Fabienne Weigel, Jochen Weiss, Eric Andrew Decker, David Julian McClements
The impact of emulsifier type (quillaja saponin, Tween 80, whey protein and casein) and antioxidant type (EDTA, ascorbic acid, catechin, alpha tocopherol, ascorbic acid palmitate) on the physical and chemical stability of lutein-fortified emulsions was investigated. Quillaja saponin produced emulsions with the best overall stability to droplet aggregation, creaming, and colour fading during storage at 45°C for ten days. The impact of antioxidant type on the stability of lutein-fortified emulsions prepared using quillaja saponin was therefore investigated further...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29033945/improved-efficacy-of-oral-immunotherapy-using-non-digestible-oligosaccharides-in-a-murine-cow-s-milk-allergy-model-a-potential-role-for-foxp3-regulatory-t-cells
#12
Marlotte M Vonk, Mara A P Diks, Laura Wagenaar, Joost J Smit, Raymond H H Pieters, Johan Garssen, Betty C A M van Esch, Léon M J Knippels
BACKGROUND: Oral immunotherapy (OIT) is a promising therapeutic approach to treat food allergic patients. However, there are some concerns regarding its safety and long-term efficacy. The use of non-digestible oligosaccharides might improve OIT efficacy since they are known to directly modulate intestinal epithelial and immune cells in addition to acting as prebiotics. AIM: To investigate whether a diet supplemented with plant-derived fructo-oligosaccharides (FOS) supports the efficacy of OIT in a murine cow's milk allergy model and to elucidate the potential mechanisms involved...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29033851/effects-of-alpha-lactalbumin-or-whey-protein-isolate-on-muscle-damage-muscle-pain-and-mood-states-following-prolonged-strenuous-endurance-exercise
#13
Lu Qin, Stephen H S Wong, Feng-Hua Sun, Yu Huang, Sinead Sheridan, Cindy H P Sit
This study compared the effect of alpha-lactalbumin and whey protein on muscle damage, muscle pain, and mood states during short term recovery following strenuous prolonged exercise. In a two-stage crossover counterbalanced design, 12 endurance male runners were recruited (age: 30.4 ± 2.8 year, height: 172.7 ± 5.6 cm, body mass: 66.7 ± 6.5 kg, VO2max: 58.0 ± 6.9 ml/kg(-)/min), ran for 90 min at 70% VO2max, and followed by a 4-h recovery. Two treatments (carbohydrate+alpha-lactalbumin, CA; carbohydrate+whey protein isolate, CW) were applied during the main trials...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29033850/designing-dietary-recommendations-using-system-level-interactomics-analysis-and-network-based-inference
#14
Tingting Zheng, Yueqiong Ni, Jun Li, Billy K C Chow, Gianni Panagiotou
Background: A range of computational methods that rely on the analysis of genome-wide expression datasets have been developed and successfully used for drug repositioning. The success of these methods is based on the hypothesis that introducing a factor (in this case, a drug molecule) that could reverse the disease gene expression signature will lead to a therapeutic effect. However, it has also been shown that globally reversing the disease expression signature is not a prerequisite for drug activity. On the other hand, the basic idea of significant anti-correlation in expression profiles could have great value for establishing diet-disease associations and could provide new insights into the role of dietary interventions in disease...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29032730/biological-groundwater-treatment-for-hexavalent-chromium-removal-at-low-chromium-concentrations-under-anoxic-conditions
#15
E Panousi, D Mamais, C Noutsopoulos, K Mpertoli, C Kantzavelou, E Nyktari, I Kavallari, M Nasioka, A Kaldis
The objective of this work is to evaluate biological groundwater treatment systems that will achieve hexavalent chromium removal from groundwater at hexavalent chromium (Cr(VI)) groundwater concentrations in the 0 - 200 μg/L range under anoxic conditions. The effect of type of organic substrate added as feed to the groundwater treatment system (milk, sugar, cheese whey), the effect of different concentrations of COD added in the feed (100, 150, 200 mg/L) and the effect of different hydraulic residence time (1...
October 14, 2017: Environmental Technology
https://www.readbyqxmd.com/read/29032473/oligosaccharide-biotechnology-an-approach-of-prebiotic-revolution-on-the-industry
#16
REVIEW
Mario Cezar Rodrigues Mano, Iramaia Angélica Neri-Numa, Juliana Bueno da Silva, Bruno Nicolau Paulino, Marina Gabriel Pessoa, Gláucia Maria Pastore
Oligosaccharides are polymers with two to ten monosaccharide residues which have sweetener functions and sensory characteristics, in addition to exerting physiological effects on human health. The ones called nondigestible exhibit a prebiotic behavior being fermented by colonic microflora or stimulating the growth of beneficial bacteria, playing roles in the immune system, protecting against cancer, and preventing cardiovascular and metabolic issues. The global prebiotics market is expected to grow around 12...
October 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29031484/effects-of-leucine-enriched-essential-amino-acid-and-whey-protein-bolus-dosing-upon-skeletal-muscle-protein-synthesis-at-rest-and-after-exercise-in-older-women
#17
Daniel J Wilkinson, Syed S I Bukhari, Bethan E Phillips, Marie C Limb, Jessica Cegielski, Matthew S Brook, Debbie Rankin, William K Mitchell, Hisamine Kobayashi, John P Williams, Jonathan Lund, Paul L Greenhaff, Kenneth Smith, Philip J Atherton
BACKGROUND & AIMS: Impaired anabolic responses to nutrition and exercise contribute to loss of skeletal muscle mass with ageing (sarcopenia). Here, we tested responses of muscle protein synthesis (MPS), in the under represented group of older women, to leucine-enriched essential amino acids (EAA) in comparison to a large bolus of whey protein (WP). METHODS: Twenty-four older women (65 ± 1 y) received (N = 8/group) 1.5 g leucine-enriched EAA supplements (LEAA_1...
September 23, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/29031397/modulation-of-the-biomarkers-of-inflammation-and-oxidative-stress-by-ruminant-trans-fatty-acids-and-dairy-proteins-in-vascular-endothelial-cells-huvec
#18
Marine S Da Silva, Jean-François Bilodeau, Jessica Larose, Karine Greffard, Pierre Julien, Olivier Barbier, Iwona Rudkowska
This study aimed to determine whether dairy macronutrients alter markers of inflammation and oxidative stress in endothelial cells. Human endothelial cells (HUVEC) were treated with ruminant trans fatty acids (rTFA), either trans-vaccenic acid (tVA) or trans-palmitoleic acid (tPA), whey protein hydrolysate, leucine or combinations of rTFA and dairy protein compounds. Industrial TFA elaidic acid (EA) was also investigated and compared with rTFA. Inflammatory prostaglandins (PG) and F2-isoprostanes (F2-isoP) isomers, markers of oxidative stress, were assessed in cell supernatants by LC-MS/MS...
November 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
https://www.readbyqxmd.com/read/29026733/safety-of-a-new-synbiotic-starter-formula
#19
Yvan Vandenplas, Antonis Analitis, Chara Tziouvara, Athina Kountzoglou, Anastasia Drakou, Manos Tsouvalas, Antigoni Mavroudi, Ioannis Xinias
PURPOSE: Breastfeeding is the best way to feed all infants, but not all infants can be (exclusively) breastfed. Cow's milk based infant formula is the second choice infant feeding. METHODS: The safety of a new synbiotic infant formula, supplemented with Bifidobacterium lactis and fructo-oligosaccharides, with lactose and a whey/casein 60/40 protein ratio was tested in 280 infants during 3 months. RESULTS: The median age of the infants at inclusion was 0...
September 2017: Pediatric Gastroenterology, Hepatology & Nutrition
https://www.readbyqxmd.com/read/29021893/the-efficacy-of-oral-and-subcutaneous-antigen-specific-immunotherapy-in-murine-cow-s-milk-and-peanut-allergy-models
#20
Marlotte M Vonk, Laura Wagenaar, Raymond H H Pieters, Leon M J Knippels, Linette E M Willemsen, Joost J Smit, Betty C A M van Esch, Johan Garssen
BACKGROUND: Antigen-specific immunotherapy (AIT) is a promising therapeutic approach for both cow's milk allergy (CMA) and peanut allergy (PNA), but needs optimization in terms of efficacy and safety. AIM: Compare oral immunotherapy (OIT) and subcutaneous immunotherapy (SCIT) in murine models for CMA and PNA and determine the dose of allergen needed to effectively modify parameters of allergy. METHODS: Female C3H/HeOuJ mice were sensitized intragastrically (i...
2017: Clinical and Translational Allergy
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