Erica L Stover, Marguerite B Little, Kristie L Connolly, Lixin Li, Robert A Nicholas, Aleksandra E Sikora, Ann E Jerse, Marcia M Hobbs, J Alex Duncan, Andrew N Macintyre
There is an urgent need for vaccines against Neisseria gonorrhoeae (Ng), the causative agent of gonorrhea. Vaccination with an outer-membrane vesicle (OMV)-based Neisseria meningitidis (Nm) vaccine provides some protection from Ng; however, the mechanisms underlying this cross-protection are unknown. To address this need, we developed multiplexed bead-based assays for the relative quantification of human and mouse IgG and IgA against Ng antigens. The assays were evaluated for analyte independence, dilutional linearity, specificity, sensitivity, intra- and inter-assay variability, and robustness to sample storage conditions...
March 25, 2024: Journal of Infectious Diseases