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https://www.readbyqxmd.com/read/28640607/primary-and-photochemically-aged-aerosol-emissions-from-biomass-cookstoves-chemical-and-physical-characterization
#1
Stephen M Reece, Aditya Sinha, Andrew P Grieshop
Secondary organic aerosol (SOA) formation during photo-oxidation of primary emissions from cookstoves used in developing countries may make important contributions to their climate and air quality impacts. We present results from laboratory experiments with a field portable oxidation flow reactor (F-OFR) to study the evolution of emissions over hours to weeks of equivalent atmospheric aging. Lab tests, using dry red oak, measured fresh and aged emissions from a 3 stone fire (TSF), a "rocket" natural draft stove (NDS) and a forced draft gasifier stove (FDGS), in order of increasing modified combustion efficiency (MCE) and decreasing particulate matter emission factors (EF)...
June 22, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28633525/use-of-dimethyl-pimelimidate-with-microfluidic-system-for-nucleic-acids-extraction-without-electricity
#2
Choong Eun Jin, Tae Yoon Lee, Bonhan Koo, Kyung-Chul Choi, Suhwan Chang, Se Yoon Park, Ji Yeun Kim, Sung-Han Kim, Yong Shin
The isolation of nucleic acids in the lab on a chip is crucial to achieve the maximal effectiveness of point-of-care testing for detection in clinical applications. Here, we report on the use of a simple and versatile single-channel microfluidic platform that combines dimethyl pimelimidate (DMP) for nucleic acids (both RNA and DNA) extraction without electricity using thin films system. The system is based on the adaption of DMP into non-chaotropic-based nucleic acids and the capture of reagents into a low-cost thin film platform for use as a microfluidic total analysis system, which can be utilized for sample processing in clinical diagnostics...
June 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28632297/n%C3%A3-lbiopsi-%C3%A3-r-inte-b%C3%A3-sta-metod-f%C3%A3-r-att-diagnostisera-lymfom-den-%C3%A3-kade-anv%C3%A3-ndningen-kan-ge-allvarliga-konsekvenser-f%C3%A3-r-diagnostik-forskning-och-behandlingsutveckling
#3
Rose-Marie Amini, Christer Sundström
Core needle biopsies for lymphoma diagnosis seriously affect diagnostics, treatment development and research Core needle biopsies (CNBs) are widely used in clinical diagnostic labs to aid in the diagnosis of malignant lymphomas and in latter years their use is increasing. CNBs provide a rapid method for obtaining tumour material and may be beneficial when the affected lymph nodes are located deep in the abdominal cavity or mediastinum and surgical excisional biopsies may be difficult to perform. However, according to the Swedish Haematopathology Quality and Standardization Committee, CNBs are insufficient for lymphoma diagnostic purposes and the guidelines state that material from surgical excisional biopsies are mandatory in order to obtain a robust histopathological evaluation of the lymph node architecture, cellular composition and growth pattern...
June 16, 2017: Läkartidningen
https://www.readbyqxmd.com/read/28629682/aquatic-live-animal-radiotracing-studies-for-ecotoxicological-applications-addressing-fundamental-methodological-deficiencies
#4
Tom Cresswell, Marc Metian, Lisa A Golding, Mike D Wood
The use of live animal gamma radioisotope tracer techniques in the field of ecotoxicology allows laboratory studies to accurately monitor contaminant biokinetics in real time for an individual organism. However, methods used in published studies for aquatic organisms are rarely described in sufficient detail to allow for study replication or an assessment of the errors associated with live animal radioanalysis to be identified. We evaluate the influence of some important methodological deficiencies through an overview of the literature on live aquatic animal radiotracer techniques and through the results obtained from our radiotracer studies on four aquatic invertebrate species...
June 16, 2017: Journal of Environmental Radioactivity
https://www.readbyqxmd.com/read/28629462/treatment-decisions-for-older-adults-with-advanced-chronic-kidney-disease
#5
REVIEW
Steven J Rosansky, Jane Schell, Joseph Shega, Jennifer Scherer, Laurie Jacobs, Cecile Couchoud, Deidra Crews, Matthew McNabney
Dialysis initiation rates among older adults, aged 75 years or greater, are increasing at a faster rate than for younger age groups. Older adults with advanced CKD (eGFR < 30 ml/min/1.73 m(2)) typically lose renal function slowly, often suffer from significant comorbidity and thus may die from associated comorbidities before they require dialysis.A patient's pattern of renal function loss over time in relation to their underlying comorbidities can serve as a guide to the probability of a future dialysis requirement...
June 19, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28626449/characterization-of-feruloyl-esterases-produced-by-the-four-lactobacillus-species-l-amylovorus-l-acidophilus-l-farciminis-and-l-fermentum-isolated-from-ensiled-corn-stover
#6
Zhenshang Xu, Huiying He, Susu Zhang, Tingting Guo, Jian Kong
Lactic acid bacteria (LAB) play important roles in silage fermentation, which depends on the production of sufficient organic acids to inhibit the growth of undesirable microorganisms. However, LAB are not able to degrade cellulose and hemicellulose. Bacteria and fibrolytic enzymes are usually used as inoculants to improve the silage quality and digestibility. In the present study, we isolated four Lactobacillus strains (L. amylovorus CGMCC 11056, L. acidophilus CCTCC AB2010208, L. farciminis CCTCC AB2016237 and L...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28623022/heterogeneity-of-zeolite-combined-with-biochar-properties-as-a-function-of-sewage-sludge-composting-and-production-of-nutrient-rich-compost
#7
Mukesh Kumar Awasthi, Meijing Wang, Ashok Pandey, Hongyu Chen, Sanjeev Kumar Awasthi, Quan Wang, Xiuna Ren, Altaf Hussain Lahori, Dong-Sheng Li, Ronghua Li, Zengqiang Zhang
In the present study, biochar combined with a higher dosage of zeolite (Z) and biochar (B) alone were applied as additives for dewatered fresh sewage sludge (DFSS) composting using 130-L working volume lab-scale reactors. We first observed that the addition of a mixture of B and Z to DFSS equivalent to 12%B+10% (Z-1), 15% (Z-2) and 30% (Z-3) zeolite (dry weight basis) worked synergistically as an amendment and increased the composting efficiency compared with a treatment of 12%B alone amended and a control without any amendment...
June 13, 2017: Waste Management
https://www.readbyqxmd.com/read/28611986/scipad-effective-implementation-of-telemedicine-using-ipads-with-individuals-with-spinal-cord-injuries-a-case-series
#8
Kazuko Shem, Samantha J Sechrist, Eleanor Loomis, Linda Isaac
BACKGROUND: Individuals with spinal cord injury (SCI) must often travel long distances to see a rehabilitation specialist. While telemedicine (TM) for pressure ulcer management has been used in this population, real-time video telecommunication using iPad has never been described. OBJECTIVE: The objective of this study was to provide specialized care for persons with SCI through TM consultation expediently in order to address medical needs, manage secondary complications, and to improve quality of life (QoL) of individuals with SCI...
2017: Frontiers in Medicine
https://www.readbyqxmd.com/read/28611499/lactobacillus-futsaii-cs3-a-new-gaba-producing-strain-isolated-from-thai-fermented-shrimp-kung-som
#9
Chatthaphisuth Sanchart, Onnicha Rattanaporn, Dietmar Haltrich, Pimpimol Phukpattaranont, Suppasil Maneerat
Kung-Som is a popular traditional Thai fermented shrimp product. It is rich in glutamic acid, which is the major substrate for the biosynthesis of gamma-aminobutyric acid (GABA) by lactic acid bacteria (LAB). In the present study, LAB from Kung-Som were isolated, screened for GABA formation, and the two isolates that transform glutamic acid most efficiently into GABA were identified. Based on the API-CHL50 fermentation profile and a phylogenetic tree of 16S rDNA sequences, strain CS3 and CS5 were identified as Lactobacillus futsaii, which was for the first time shown to be a promising GABA producer...
June 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28608152/recovering-from-chronic-myeloid-leukemia-the-patients-perspective-seen-through-the-lens-of-narrative-medicine
#10
G Graffigna, I Cecchini, M Breccia, E Capochiani, R Della Seta, S Galimberti, A Melosi, F Simonetti, M Pizzuti, S F Capalbo, F Falzetti, P Mazza, N Di Renzo, L Mastrullo, D Rapezzi, E Orlandi, T Intermesoli, A Iurlo, E Pungolino, M Pacilli
PURPOSE: The main objective of this study is to gain a deeper understanding of how patients suffering from chronic myeloid leukemia (CML) cope with their illness. The study aims to reconstruct the subjective meaning-making process related to CML in order to gain insights into the impact the disease has on patients' emotions and everyday lives, as well as to explore the psychological impact of their being presented with the chance to suspend their therapy and recover from the disease. METHODS: Data were gathered from a qualitative study conducted in Italy on 158 Italian CML patients...
June 12, 2017: Quality of Life Research
https://www.readbyqxmd.com/read/28603613/solid-state-fermentation-of-corn-soybean-meal-mixed-feed-with-bacillus-subtilis-and-enterococcus-faecium-for-degrading-antinutritional-factors-and-enhancing-nutritional-value
#11
Changyou Shi, Yu Zhang, Zeqing Lu, Yizhen Wang
BACKGROUND: Corn and soybean meal (SBM) are two of the most common feed ingredients used in pig feeds. However, a variety of antinutritional factors (ANFs) present in corn and SBM can interfere with the bioavailability of nutrients and have negative health effects on the pigs. In the present study, two-stage fermentation using Bacillus subtilis followed by Enterococcus faecium was carried out to degrade ANFs and improve the nutritional quality of corn and SBM mixed feed. Furthermore, the microbial composition and in vitro nutrient digestibility of inoculated mixed feed were determined and compared those of the uninoculated controls...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/28602613/cryopause-a-new-method-to-immediately-initiate-experiments-after-cryopreservation-of-pluripotent-stem-cells
#12
Karen G Wong, Sean D Ryan, Kiran Ramnarine, Siera A Rosen, Shannon E Mann, Amanda Kulick, Elisa De Stanchina, Franz-Josef Müller, Thadeous J Kacmarczyk, Chao Zhang, Doron Betel, Mark J Tomishima
Human pluripotent stem cells (PSCs) provide an unlimited cell source for cell therapies and disease modeling. Despite their enormous power, technical aspects have hampered reproducibility. Here, we describe a modification of PSC workflows that eliminates a major variable for nearly all PSC experiments: the quality and quantity of the PSC starting material. Most labs continually passage PSCs and use small quantities after expansion, but the "just-in-time" nature of these experiments means that quality control rarely happens before use...
June 6, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28594204/taking-forensic-mental-health-assessment-out-of-the-lab-and-into-the-real-world-introduction-to-the-special-issue-on-the-field-utility-of-forensic-assessment-instruments-and-procedures
#13
John F Edens, Marcus T Boccaccini
The last several decades have seen a major upswing in the development and use of psychological assessment instruments in forensic and correctional settings. At the same time, admissibility standards increasingly have stressed the importance of the reliability and validity of evidence in legal proceedings. Recent research has, however, raised serious concerns about (a) the reliability of forensic science evidence in general, (b) the replicability of psychological research findings in general and in field settings especially, and (c) the interrater reliability and predictive validity of forensic psychological assessment evidence in particular...
June 2017: Psychological Assessment
https://www.readbyqxmd.com/read/28592266/urgent-needs-in-fostering-neglected-tropical-diseases-ntds-laboratory-capacity-in-who-western-pacific-region-results-from-the-external-quality-assessment-on-ntds-diagnosis-in-2012-2015
#14
Yan Lu, Glenda Gonzales, Shao-Hong Chen, Hao Li, Yu-Chun Cai, Yan-Hong Chu, Lin Ai, Mu-Xin Chen, Hai-Ning Chen, Jia-Xu Chen
BACKGROUND: Neglected tropical diseases (NTDs) are a heterogeneous group of mainly chronic, debilitating and often stigmatizing diseases that largely affects low-income and politically marginalized populations, causing a large burden of public health, social and economies in the NTDs endemic countries. NTDs are caused by infections with a range of pathogen, including bacteria, parasites, protozoa and viruses. The accurate diagnosis of NTDs is important for reducing morbidity, preventing mortality and for monitoring of control programs...
June 8, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28589305/discriminating-ability-of-abbreviated-impactor-measurement-approach-aim-to-detect-changes-in-mass-median-aerodynamic-diameter-mmad-of-an-albuterol-salbutamol-pmdi-aerosol
#15
J David Christopher, Rajni B Patel, Jolyon P Mitchell, Terrence P Tougas, Adrian P Goodey, Jorge Quiroz, Patrik U Andersson, Svetlana Lyapustina
This article reports on results from a two-lab, multiple impactor experiment evaluating the abbreviated impactor measurement (AIM) concept, conducted by the Cascade Impaction Working Group of the International Pharmaceutical Aerosol Consortium on Regulation and Science (IPAC-RS). The goal of this experiment was to expand understanding of the performance of an AIM-type apparatus based on the Andersen eight-stage non-viable cascade impactor (ACI) for the assessment of inhalation aerosols and sprays, compared with the full-resolution version of that impactor described in the pharmacopeial compendia...
June 6, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28579365/a-novel-python-program-for-implementation-of-quality-control-in-elisa
#16
Hanna N Wetzel, Cinder Cohen, Andrew B Norman, Rose P Webster
The use of semi-quantitative assays such as the enzyme-linked immunosorbent assay (ELISA) requires stringent quality control of the data. However, such quality control is often lacking in academic settings due to unavailability of software and knowledge. Therefore, our aim was to develop methods to easily implement Levey-Jennings quality control methods. For this purpose, we created a program written in Python (a programming language with an open source license) and tested it using a training set of ELISA standard curves quantifying the Fab fragment of an anti-cocaine monoclonal antibody in mouse blood...
June 1, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28578265/lactic-acid-bacteria-involved-in-cocoa-beans-fermentation-from-ivory-coast-species-diversity-and-citrate-lyase-production
#17
Hadja D Ouattara, Honoré G Ouattara, Michel Droux, Sylvie Reverchon, William Nasser, Sébastien L Niamke
Microbial fermentation is an indispensable process for high quality chocolate from cocoa bean raw material. lactic acid bacteria (LAB) are among the major microorganisms responsible for cocoa fermentation but their exact role remains to be elucidated. In this study, we analyzed the diversity of LAB in six cocoa producing regions of Ivory Coast. Ribosomal 16S gene sequence analysis showed that Lactobacillus plantarum and Leuconostoc mesenteroides are the dominant LAB species in these six regions. In addition, other species were identified as the minor microbial population, namely Lactobacillus curieae, Enterococcus faecium, Fructobacillus pseudoficulneus, Lactobacillus casei, Weissella paramesenteroides and Weissella cibaria...
May 17, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28577274/unexpected-events-disrupt-visuomotor-working-memory-and-increase-guessing
#18
R Dawn Finzi, Bradley R Postle, Timothy F Brady, Adam R Aron
When an unexpected event, such as a car horn honking, occurs in daily life, it often disrupts our train of thought. In the lab, this effect was recently modeled with a task in which verbal working memory (WM) was disrupted by unexpected auditory events (Wessel et al. in Nature Communications, 7, 11195, 2016). Here we tested whether this effect extends to a different type of WM-namely, visuomotor. We found that unexpected auditory events similarly decremented visuomotor WM. Moreover, this effect persisted for many more trials than had previously been shown for verbal WM, and the effect occurred for two different types of unexpected auditory events...
June 2, 2017: Psychonomic Bulletin & Review
https://www.readbyqxmd.com/read/28576374/molecular-investigation-of-bacterial-communities-during-the-manufacturing-and-ripening-of-semi-hard-iranian-liqvan-cheese
#19
M Ramezani, S M Hosseini, I Ferrocino, M A Amoozegar, L Cocolin
Liqvan (or Lighvan) is a traditional Iranian cheese from the East Azerbaijan province of Iran, which is made of raw ewe's milk without the addition of a starter. The grazing pastures, environmental conditions and the ancient regional production methods allocate a distinctive microbial ecology to this type of cheese, and these factors are consequently associated with the quality of the product. In this study, the microbiota of the milk, curd and cheese has been investigated using culture independent approaches...
September 2017: Food Microbiology
https://www.readbyqxmd.com/read/28575708/education-of-student-nurses-a-systematic-literature-review
#20
REVIEW
Kathrine Håland Jeppesen, Sytter Christiansen, Kirsten Frederiksen
OBJECTIVE: The aim of this review was to explore the literature on the connection between teaching strategies and nursing students' learning to clarify which teaching strategies provide optimal learning experiences and outcomes. DATA SOURCES: Sources dating from January 2000 to November 2016 were systematically searched in PubMed, Cinahl, Scopus, Web of Science, ProQuest. METHODS: A systematic literature review was conducted that included quantitative and qualitative studies...
May 13, 2017: Nurse Education Today
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