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https://www.readbyqxmd.com/read/29769498/quantitative-analysis-of-nucleic-acid-extraction-methods-for-vibrio-cholerae-using-real-time-pcr-and-conventional-pcr
#1
Z Z Hossain, J Ferdous, S M Tulsiani, P M Jensen, A Begum
The devastating diarrheal disease cholera is caused by the bacterium Vibrio cholerae. Nucleic acid extraction is the primary step for several molecular detection approaches. In order to identify the sources of cholera illness, an efficient, fast and easy DNA extraction method for toxigenic Vibrio cholerae is desired which can be applied on diverse type of samples. This methodology developmental setup study was performed in the Environmental Microbiology Lab, Department of Microbiology, University of Dhaka, Bangladesh from March 2015 to May 2015 attempted to compare three DNA extraction methods for efficient detection and quantification of Vibrio cholerae...
April 2018: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/29769305/first-person-pawel-leznicki
#2
(no author information available yet)
First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Pawel Leznicki is the first author on 'Expansion of DUB functionality generated by alternative isoforms - USP35, a case study', published in Journal of Cell Science. Pawel conducted the research in this article while in Yogesh Kulathu's lab at the University of Dundee, UK. He is now a research associate in the lab of Stephen High at the University of Manchester, UK, investigating protein biogenesis processes and their quality control...
May 16, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/29768030/evaluation-of-petrifilm-lactic-acid-bacteria-plates-for-counting-lactic-acid-bacteria-in-food
#3
Satomi Kanagawa, Chihiro Ohshima, Hajime Takahashi, Burenqiqige, Misato Kikuchi, Fumina Sato, Ayaka Nakamura, Shimaa M Mohamed, Takashi Kuda, Bon Kimura
Although lactic acid bacteria (LAB) are used widely as starter cultures in the production of fermented foods, they are also responsible for food decay and deterioration. The undesirable growth of LAB in food causes spoilage, discoloration, and slime formation. Because of these adverse effects, food companies test for the presence of LAB in production areas and processed foods and consistently monitor the behavior of these bacteria. The 3M Petrifilm LAB Count Plates have recently been launched as a time-saving and simple-to-use plate designed for detecting and quantifying LAB...
May 16, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29767357/a-guide-for-designing-and-analyzing-rna-seq-data
#4
Aniruddha Chatterjee, Antonio Ahn, Euan J Rodger, Peter A Stockwell, Michael R Eccles
The identity of a cell or an organism is at least in part defined by its gene expression and therefore analyzing gene expression remains one of the most frequently performed experimental techniques in molecular biology. The development of the RNA-Sequencing (RNA-Seq) method allows an unprecedented opportunity to analyze expression of protein-coding, noncoding RNA and also de novo transcript assembly of a new species or organism. However, the planning and design of RNA-Seq experiments has important implications for addressing the desired biological question and maximizing the value of the data obtained...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29753036/explaining-the-amount-and-consistency-of-medical-care-and-self-management-support-in-asthma-a-survey-of-primary-care-providers-in-france-and-the-united-kingdom
#5
Marijn de Bruin, Alexandra L Dima, Nathalie Texier, Eric van Ganse
BACKGROUND: The quality of asthma primary care may vary between countries, health care practices, and health care professionals (HCPs). Identifying and explaining such differences is critical for health services improvement. OBJECTIVES: To examine the quality of asthma primary care in France and United Kingdom, and identify within-country and between-country predictors amenable to intervention. METHODS: An online questionnaire to capture asthma medical care and self-management support, practice characteristics, and psychosocial determinants, was completed by 276 HCPs...
May 9, 2018: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/29749996/absorption-refrigeration-cycles-with-ammonia-ionic-liquid-working-pairs-studied-by-molecular-simulation
#6
Tim M Becker, Meng Wang, Abhishek Kabra, Seyed Hossein Jamali, Mahinder Ramdin, David Dubbeldam, Carlos A Infante Ferreira, Thijs J H Vlugt
For absorption refrigeration, it has been shown that ionic liquids have the potential to replace conventional working pairs. Due to the huge number of possibilities, conducting lab experiments to find the optimal ionic liquid is infeasible. Here, we provide a proof-of-principle study of an alternative computational approach. The required thermodynamic properties, i.e., solubility, heat capacity, and heat of absorption, are determined via molecular simulations. These properties are used in a model of the absorption refrigeration cycle to estimate the circulation ratio and the coefficient of performance...
April 18, 2018: Industrial & Engineering Chemistry Research
https://www.readbyqxmd.com/read/29732324/retrospective-study-on-the-hygienic-quality-of-fresh-ricotta-cheeses-produced-in-sicily-italy
#7
Maria Luisa Scatassa, Isabella Mancuso, Sonia Sciortino, Giusi Macaluso, Marisa Palmeri, Luigi Arcuri, Massimo Todaro, Cinzia Cardamone
The present work was carried out to investigate the microbiological profile of Sicilian ewes' ricotta cheeses during fifteen years of investigations (2002-2016). The samples were collected between those conferred to the Istituto Zooprofilattico Sperimentale della Sicilia (IZSSi) Adelmo Mirri , Palermo (Italy), by the competent authority during official control, by food business operator in HACCP systems and in research projects. Enterobacteriaceae, Escherichia coli and coagulase - positive staphylococci (CPS) were found only in some samples...
March 31, 2018: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/29730488/the-use-of-wearable-devices-for-walking-and-running-gait-analysis-outside-of-the-lab-a-systematic-review
#8
REVIEW
Lauren C Benson, Christian A Clermont, Eva Bošnjak, Reed Ferber
BACKGROUND: Quantitative gait analysis is essential for evaluating walking and running patterns for markers of pathology, injury, or other gait characteristics. It is expected that the portability, affordability, and applicability of wearable devices to many different populations will have contributed advancements in understanding the real-world gait patterns of walkers and runners. Therefore, the purpose of this systematic review was to identify how wearable devices are being used for gait analysis in out-of-lab settings...
May 1, 2018: Gait & Posture
https://www.readbyqxmd.com/read/29728819/the-changing-tides-of-irish-orthopaedic-research
#9
Ailbhe White Gibson, Kevin Clesham, Oisin Tully, John F Quinlan
INTRODUCTION: Research is fundamental to bridging theory, practice and education in orthopaedics. Following the restructuring of the surgical training pathway in Ireland, the opportunity to undertake clinical- or lab-based research has fallen. AIMS: Our aim was to investigate the trends of research in orthopaedics and the implications there. METHODS: We reviewed the trend in publications by Irish trauma and orthopaedic (T&O) trainees over the past 20 years across three different classes of journal...
May 4, 2018: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/29727857/unravelling-the-contribution-of-lactic-acid-bacteria-and-acetic-acid-bacteria-to-cocoa-fermentation-using-inoculated-organisms
#10
Van Thi Thuy Ho, Graham H Fleet, Jian Zhao
Cocoa beans (Theobroma cacao L.) are the raw material for chocolate production. Fermentation of the bean pulp by microorganisms is essential for developing the precursors of chocolate flavour. Currently, the cocoa fermentation is still conducted by an uncontrolled traditional process via a consortium of indigenous species of yeasts, lactic acid bacteria and acetic acid bacteria. Although the essential contribution of yeasts to the production of good quality beans and, typical chocolate character is generally agreed, the roles of lactic acid bacteria and acetic acid bacteria are less certain...
April 27, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29725937/public-lab-community-based-approaches-to-urban-and-environmental-health-and-justice
#11
Pablo Rey-Mazón, Hagit Keysar, Shannon Dosemagen, Catherine D'Ignazio, Don Blair
This paper explores three cases of Do-It-Yourself, open-source technologies developed within the diverse array of topics and themes in the communities around the Public Laboratory for Open Technology and Science (Public Lab). These cases focus on aerial mapping, water quality monitoring and civic science practices. The techniques discussed have in common the use of accessible, community-built technologies for acquiring data. They are also concerned with embedding collaborative and open source principles into the objects, tools, social formations and data sharing practices that emerge from these inquiries...
May 3, 2018: Science and Engineering Ethics
https://www.readbyqxmd.com/read/29710573/the-application-of-gac-sandwich-slow-sand-filtration-to-remove-pharmaceutical-and-personal-care-products
#12
Jianan Li, Qizhi Zhou, Luiza C Campos
Lab-scale GAC sandwich slow sand filters with different GAC layer depths were evaluated for the first time to remove selected pharmaceutical and personal care products (PPCPs) (namely DEET, paracetamol, caffeine and triclosan, 25 μg/L). Coarse sand (effective grain size of 0.6 mm) was used instead of conventional fine sand. In addition to single sand and GAC filters, GAC sandwich filters were assessed at three filtration rates (i.e. 5 cm/h, 10 cm/h and 20 cm/h) to compare removals. Sandwich filter with 20 cm GAC achieved the best average PPCP removal (98...
April 22, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29706016/role-of-local-anaesthetic-nerve-block-in-endoscopic-sinus-surgery-a-systematic-review-and-meta-analysis
#13
E Shamil, M J Rouhani, S Basetti, F Bast, C Hopkins, P Surda
OBJECTIVE: The aim of the study was to perform a systematic review of existing evidence on the role of local anaesthetic nerve block (LAB) in patients undergoing endoscopic sinus surgery (ESS). DESIGN: The databases searched were the Cochrane Register of Controlled Trials, MEDLINE and Embase using the Ovid portal (1946-2017). RESULTS: Seven randomised controlled trials were included. Due to considerable heterogeneity of data, only two studies were pooled into meta-analysis which demonstrated a statistically significantly better surgical field quality during ESS in the LAB group compared to the control group (MD -0...
April 29, 2018: Clinical Otolaryngology
https://www.readbyqxmd.com/read/29704977/outcomes-of-an-innovative-evidence-based-practice-project-building-a-difficult-access-team-in-the-emergency-department
#14
Madeleine Whalen, Barbara Maliszewski, Rebecca Sheinfeld, Heather Gardner, Diana Baptiste
PROBLEM: Difficult venous access is a common problem in health care-especially in the emergency setting-that relies on quick diagnostics to differentiate patient acuities and administer critical medications. The creation of a dedicated team to address difficult venous access (DVA) is a possible solution to the problems of delayed venous access, yet no studies have been published on implementing such a team in the emergency department. METHODS: This was a quasi-experimental study in an urban emergency department...
April 25, 2018: Journal of Emergency Nursing: JEN: Official Publication of the Emergency Department Nurses Association
https://www.readbyqxmd.com/read/29696971/a-simple-chip-electrophoresis-titration-of-neutralization-boundary-with-edta-photo-catalysis-for-distance-based-sensing-of-melamine-in-dairy-products
#15
Wen-Lin Li, Fan-Zhi Kong, Qiang Zhang, Wei-Wen Liu, Hao Kong, Xiao-Ping Liu, Muhammad-Idrees Khan, Amir Wahid, Shah Saud, Hua Xiao, Cheng-Xi Cao, Liu-Yin Fan
Melamine was sometimes adulterated to dairy products for false protein content increase in developing countries. However, portable sensor has not been developed for on-spot determination of melamine in dairy products yet. Herein, a distance-based sensor was advanced for the quantification of melamine in dairy products based on chip electrophoretic titration (ET) of moving neutralization boundary (NB) and EDTA photo-catalysis. In the chip sensor, EDTA, H2O2 and leucomalachite green (LMG) were added in the anode well...
April 26, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29692621/h3africa-current-perspectives
#16
REVIEW
Nicola Mulder, Alash'le Abimiku, Sally N Adebamowo, Jantina de Vries, Alice Matimba, Paul Olowoyo, Michele Ramsay, Michelle Skelton, Dan J Stein
Precision medicine is being enabled in high-income countries by the growing availability of health data, increasing knowledge of the genetic determinants of disease and variation in response to treatment (pharmacogenomics), and the decreasing costs of data generation, which promote routine application of genomic technologies in the health sector. However, there is uncertainty about the feasibility of applying precision medicine approaches in low- and middle-income countries, due to the lack of population-specific knowledge, skills, and resources...
2018: Pharmacogenomics and Personalized Medicine
https://www.readbyqxmd.com/read/29691186/does-micropuncture-technique-really-help-reduce-vascular-complications
#17
Hari Bogabathina, Sampath Singireddy, Runhua Shi, Liam Morris, Abdulrahman Abdulbaki, Henock Zabher, Pavan Katikaneni, Kalgi Modi
BACKGROUND: Micropuncture technique (MPT) is being adapted nationally to reduce vascular complications (VC). We initiated a quality improvement project in our cath lab to reduce VCs utilizing MPT. METHODS: We utilized MPT on all of our non-STEMI femoral artery (FA) access cases starting September 2016. As a comparator group, we collected data from April to August 2016. Anatomic localization of FA and fluoroscopic marking of femoral head was utilized in all cases...
March 26, 2018: Cardiovascular Revascularization Medicine: Including Molecular Interventions
https://www.readbyqxmd.com/read/29690547/stability-of-antiradical-activity-of-protein-extracts-and-hydrolysates-from-dry-cured-pork-loins-with-probiotic-strains-of-lab
#18
Paulina Kęska, Joanna Stadnik
The application of starter cultures to improve quality and safety has become a very common practice in the meat industry. Probiotic strains of lactic acid bacteria (LAB) can also bring health benefits by releasing bioactive peptides. The aim of this work was to evaluate the stability of antiradical activity of protein extracts from LAB-inoculated dry-cured pork loins during long-term aging and evaluate their hydrolysates after simulated gastrointestinal digestion. Analyses of hydrolysates by using liquid chromatography-tandem mass spectrometry (LC-MS/MS) were strengthened with in silico analysis...
April 22, 2018: Nutrients
https://www.readbyqxmd.com/read/29689444/changes-in-the-physical-properties-of-the-dynamic-layer-and-its-correlation-with-permeate-quality-in-a-self-forming-dynamic-membrane-bioreactor
#19
Dao Guan, Ji Dai, Yoshimasa Watanabe, Guanghao Chen
The self-forming dynamic membrane bioreactor (SFDMBR) is a biological wastewater treatment technology based on the conventional membrane bioreactor (MBR) with membrane material modification to a large pore size (30-100 μm). This modification requires a dynamic layer formed by activated sludge to provide effective filtration function for high-quality permeate production. The properties of the dynamic layer are therefore important for permeate quality in SFDMBRs. The interaction between the structure of the dynamic layer and the performance of SFDMBRs is little known but understandably complex...
April 18, 2018: Water Research
https://www.readbyqxmd.com/read/29686990/perspective-of-micro-process-engineering-for-thermal-food-treatment
#20
Alexander Mathys
Micro process engineering as a process synthesis and intensification tool enables an ultra-short thermal treatment of foods within milliseconds (ms) using very high surface-area-to-volume ratios. The innovative application of ultra-short pasteurization and sterilization at high temperatures, but with holding times within the range of ms would allow the preservation of liquid foods with higher qualities, thereby avoiding many unwanted reactions with different temperature-time characteristics. Process challenges, such as fouling, clogging, and potential temperature gradients during such conditions need to be assessed on a case by case basis and optimized accordingly...
2018: Frontiers in Nutrition
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