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https://www.readbyqxmd.com/read/28092820/pcr-screening-of-an-african-fermented-pearl-millet-porridge-metagenome-to-investigate-the-nutritional-potential-of-its-microbiota
#1
Fabien Saubade, Christèle Humblot, Youna M Hemery, Jean-Pierre Guyot
Cereals are staple foods in most African countries, and many African cereal-based foods are spontaneously fermented. The nutritional quality of cereal products can be enhanced through fermentation, and traditional cereal-based fermented foods (CBFFs) are possible sources of lactic acid bacteria (LAB) with useful nutritional properties. The nutritional properties of LAB vary depending on the species and even on the strain, and the microbial composition of traditional CBFFs varies from one traditional production unit (TPU) to another...
December 29, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28090096/a-bioinformatics-practicum-to-develop-student-understanding-of-immunological-rejection-of-protein-drugs
#2
Lauren A O'Donnell, Wilson S Meng, Benjamin J Andrick, Alexa M Borello
Objectives. To design and implement a bioinformatics exercise that applies immunological principles to predicting rejection of protein drugs based upon patient genotype. Design. Doctor of pharmacy (PharmD) students used the Immune Epitope Database, a freely available bioinformatics tool. Over a 2-week laboratory, students interrogated whether a protein drug would be predicted to induce an immune response based upon patient genotype. Results were presented at the last laboratory session, and students completed reports discussing their findings...
November 25, 2016: American Journal of Pharmaceutical Education
https://www.readbyqxmd.com/read/28078255/quality-characteristics-and-antioxidant-activity-of-yogurt-supplemented-with-aronia-aronia-melanocarpa-juice
#3
Linh Nguyen, Eun-Sun Hwang
We investigated the quality characteristics and antioxidant activities of yogurt supplemented with 1%, 2%, and 3% aronia juice and fermented for 24 h at 37°C. The total acidity increased with increasing levels of aronia juice and incubation time. Lightness and yellowness of the yogurt decreased, but redness increased, with increasing aronia juice content and incubation time. The number of lactic acid bacteria (LAB) increased with increased incubation time, and yogurt containing 2% and 3% aronia juice showed higher LAB counts than 1% aroinia juice-supplemented yogurt...
December 2016: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/28066818/microbiome-helper-a-custom-and-streamlined-workflow-for-microbiome-research
#4
André M Comeau, Gavin M Douglas, Morgan G I Langille
Sequence-based approaches to study microbiomes, such as 16S rRNA gene sequencing and metagenomics, are uncovering associations between microbial taxa and a myriad of factors. A drawback of these approaches is that the necessary sequencing library preparation and bioinformatic analyses are complicated and continuously changing, which can be a barrier for researchers new to the field. We present three essential components to conducting a microbiome experiment from start to finish: first, a simplified and step-by-step custom gene sequencing protocol that requires limited lab equipment, is cost-effective, and has been thoroughly tested and utilized on various sample types; second, a series of scripts to integrate various commonly used bioinformatic tools that is available as a standalone installation or as a single downloadable virtual image; and third, a set of bioinformatic workflows and tutorials to provide step-by-step guidance and education for those new to the microbiome field...
January 2017: MSystems
https://www.readbyqxmd.com/read/28063063/identifying-candidate-reprogramming-genes-in-mouse-induced-pluripotent-stem-cells
#5
Fang Gao, Jingyu Li, Heng Zhang, Xu Yang, Tiezhu An
Factor-based induced reprogramming approaches have tremendous potential for human regenerative medicine, but the efficiencies of these approaches are still low. In this study, we analyzed the global transcriptional profiles of mouse induced pluripotent stem cells (miPSCs) and mouse embryonic stem cells (mESCs) from seven different labs and present here the first successful clustering according to cell type, not by lab of origin. We identified 2131 different expression genes (DEs) as candidate pluripotency-associated genes by comparing mESCs/miPSCs with somatic cells and 720 DEs between miPSCs and mESCs...
January 6, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28058249/microbiological-and-chemico-physical-shelf-life-and-panel-test-to-evaluate-acceptability-of-liver-mortadella
#6
Erica Tirloni, Simone Stella, Cristian Bernardi, Vittorio Maria Moretti, Carla Bersani, Patrizia Cattaneo
This study aimed to evaluate the shelf life of sliced cooked liver mortadella packaged in MAP (70-85% N2, 15-30% CO2) and stored in refrigeration (4°C) or slight thermal abuse (8°C) for up to 49 days (declared best before date 45 days). The proximate composition, aw nitrites and NaCl content were determined at T0. Weekly, samples were submitted to microbiological [total viable count (TVC), lactic acid bacteria (LAB), Enterobacteriaceae, Escherichia coli, Pseudomonas spp., coagulase positive staphylococci, sulphite reducing clostridia, yeasts and moulds, Listeria monocytogenes and Salmonella spp...
September 20, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28056760/mpd-multiplex-primer-design-for-next-generation-targeted-sequencing
#7
Thomas S Wingo, Alex Kotlar, David J Cutler
BACKGROUND: Targeted resequencing offers a cost-effective alternative to whole-genome and whole-exome sequencing when investigating regions known to be associated with a trait or disease. There are a number of approaches to targeted resequencing, including microfluidic PCR amplification, which may be enhanced by multiplex PCR. Currently, there is no open-source software that can design next-generation multiplex PCR experiments that ensures primers are unique at a genome-level and efficiently pools compatible primers...
January 5, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28052319/treatment-and-management-of-chronic-fatigue-syndrome-myalgic-encephalomyelitis-all-roads-lead-to-rome
#8
REVIEW
Jesus Castro-Marrero, Naia Sáez-Francàs, Dafna Santillo, Jose Alegre
This comprehensive review explores the current evidence on benefits and harms of therapeutic interventions in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) and makes recommendations. CFS/ME is a complex, multi-system, chronic medical condition whose pathophysiology remains unknown. No established diagnostic tests exist; nor are any FDA-approved drugs available for treatment. Because of the range of symptoms of CFS/ME, treatment approaches vary widely. Studies undertaken have heterogeneous designs and are limited by sample size, length of follow-up, applicability and methodological quality...
January 4, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28049524/maldi-tof-ms-as-an-innovative-tool-for-detection-of-plasmodium-parasites-in-anopheles-mosquitoes
#9
Maureen Laroche, Lionel Almeras, Emilie Pecchi, Yassina Bechah, Didier Raoult, Angèle Viola, Philippe Parola
BACKGROUND: Malaria is still a major public health issue worldwide, and one of the best approaches to fight the disease remains vector control. The current methods for mosquito identification include morphological methods that are generally time-consuming and require expertise, and molecular methods that require laboratory facilities with relatively expensive running costs. Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technology, routinely used for bacterial identification, has recently emerged in the field of entomology...
January 3, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28048893/mo-de-209-01-primer-on-tomosynthesis
#10
A Maidment
: Digital Breast Tomosynthesis (DBT) is rapidly replacing mammography as the standard of care in breast cancer screening and diagnosis. DBT is a form of computed tomography, in which a limited set of projection images are acquired over a small angular range and reconstructed into tomographic data. The angular range varies from 15° to 50° and the number of projections varies between 9 and 25 projections, as determined by the equipment manufacturer. It is equally valid to treat DBT as the digital analog of classical tomography - that is, linear tomography...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28048767/su-f-i-77-radiation-dose-in-cardiac-catheterization-procedures-impact-of-a-systematic-reduction-in-pulsed-fluoroscopy-frame-rate
#11
C Schultz, S Dixon
PURPOSE: To evaluate whether one small systematic reduction in fluoroscopy frame rate has a significant effect on the total air kerma and/or dose area product for diagnostic and interventional cardiac catheterization procedures. METHODS: The default fluoroscopy frame rate (FFR) was lowered from 15 to 10 fps in 5 Siemens™ Axiom Artis cardiac catheterization labs (CCL) on July 1, 2013. A total of 7212 consecutive diagnostic and interventional CCL procedures were divided into two study groups: 3602 procedures from 10/1/12 -6/30/13 with FFR of 15 fps; and 3610 procedures 7/1/13 - 3/31/14 at 10 fps...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28048241/mo-de-209-02-tomosynthesis-reconstruction-methods
#12
J Mainprize
: Digital Breast Tomosynthesis (DBT) is rapidly replacing mammography as the standard of care in breast cancer screening and diagnosis. DBT is a form of computed tomography, in which a limited set of projection images are acquired over a small angular range and reconstructed into tomographic data. The angular range varies from 15° to 50° and the number of projections varies between 9 and 25 projections, as determined by the equipment manufacturer. It is equally valid to treat DBT as the digital analog of classical tomography - that is, linear tomography...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28048094/mo-de-bra-05-eutempe-rx-combining-e-learning-and-face-to-face-training-to-build-expert-knowledge-skills-and-competences-for-medical-physicists-in-diagnostic-and-interventional-radiology
#13
H Bosmans, N Van Peteghem, A Mackenzie, E Vano, S Creten, M Borowski, S Christofides, C Caruana
PURPOSE: In 2013, the EURATOM authorities of the European Commission decided to support the Horizon2020 project submission 'EUTEMPE-RX' that aimed for a new set of course modules to train medical physicists in diagnostic and interventional radiology to expert level with small group deep learning. Each module would consist of 2 phases: an e-learning and a face-to-face phase, each phase requiring typically 40h of participant time. METHODS: The European Federation (EFOMP) and 13 European partners, all of them selected for their excellent scientific and/or educational skills, led the 12 course modules...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28048058/mo-de-209-04-radiation-dosimetry-in-breast-tomosynthesis
#14
I Sechopoulos
: Digital Breast Tomosynthesis (DBT) is rapidly replacing mammography as the standard of care in breast cancer screening and diagnosis. DBT is a form of computed tomography, in which a limited set of projection images are acquired over a small angular range and reconstructed into tomographic data. The angular range varies from 15° to 50° and the number of projections varies between 9 and 25 projections, as determined by the equipment manufacturer. It is equally valid to treat DBT as the digital analog of classical tomography - that is, linear tomography...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28047595/mo-de-209-03-assessing-image-quality
#15
W Zhao
: Digital Breast Tomosynthesis (DBT) is rapidly replacing mammography as the standard of care in breast cancer screening and diagnosis. DBT is a form of computed tomography, in which a limited set of projection images are acquired over a small angular range and reconstructed into tomographic data. The angular range varies from 15° to 50° and the number of projections varies between 9 and 25 projections, as determined by the equipment manufacturer. It is equally valid to treat DBT as the digital analog of classical tomography - that is, linear tomography...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28047354/su-f-t-77-a-novel-test-for-coincidence-between-light-fields-and-electron-radiation-fields
#16
G Gibbard, C Shang, S Khanal
PURPOSE: To validate a novel real time quality assurance device, as a means to test coincidence of light fields to electron radiation fields. METHOD AND MATERIALS: Use a Raven™ (LAP of America Laser Applications) detector, both light fields and electron radiation fields of various electron cones and cutouts from two Clinacs (Varian Medical) were sensed by the phosphor screen and registered by a CCD camera of the device. During measurements, the screen surface of Raven was placed at ISO center level facing the radiation field at any setup angle...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28046829/mo-de-209-00-dbt-physics-basic-to-advanced
#17
Andrew D Maidment
: Digital Breast Tomosynthesis (DBT) is rapidly replacing mammography as the standard of care in breast cancer screening and diagnosis. DBT is a form of computed tomography, in which a limited set of projection images are acquired over a small angular range and reconstructed into tomographic data. The angular range varies from 15° to 50° and the number of projections varies between 9 and 25 projections, as determined by the equipment manufacturer. It is equally valid to treat DBT as the digital analog of classical tomography - that is, linear tomography...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28040869/metabolic-engineering-of-klebsiella-pneumoniae-based-on-in-silico-analysis-and-its-pilot-scale-application-for-1-3-propanediol-and-2-3-butanediol-co-production
#18
Jong Myoung Park, Chelladurai Rathnasingh, Hyohak Song
Klebsiella pneumoniae naturally produces relatively large amounts of 1,3-propanediol (1,3-PD) and 2,3-butanediol (2,3-BD) along with various byproducts using glycerol as a carbon source. The ldhA and mdh genes in K. pneumoniae were deleted based on its in silico gene knockout simulation with the criteria of maximizing 1,3-PD and 2,3-BD production and minimizing byproducts formation and cell growth retardation. In addition, the agitation speed, which is known to strongly affect 1,3-PD and 2,3-BD production in Klebsiella strains, was optimized...
December 31, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28040185/fate-of-the-microbial-population-and-the-physico-chemical-parameters-of-sanganel-a-typical-blood-sausages-of-the-friuli-a-north-east-region-of-italy
#19
Lucilla Iacumin, Marisa Manzano, Simone Stella, Giuseppe Comi
In Friuli, a Northeastern region of Italy, a blood sausage called Sanganel is produced by farmers, butchers, shops, and factories. This sausage is made with pork meat, boiled blood, lard, spices, and salt. It is stored at 4 ± 2 °C and usually eaten fresh or boiled within 14 days of its manufacture. Little is known about its microbial populations and safety for consumption. The aim of this study is to characterise the microbial populations and the physico-chemical parameters of Sanganel to establish its quality and the safety of consuming it...
May 2017: Food Microbiology
https://www.readbyqxmd.com/read/28040175/impact-of-nacl-reduction-on-lactic-acid-bacteria-during-fermentation-of-nocellara-del-belice-table-olives
#20
Paola Zinno, Barbara Guantario, Giuditta Perozzi, Gianni Pastore, Chiara Devirgiliis
Table olives are widely consumed worldwide but, due to the presence of NaCl in fermenting brines, they contain high levels of sodium. A promising strategy to lower sodium content is the reduction or substitution of NaCl in brines with other chlorides. However, these procedures may impact safety, spoilage, as well as quality and technological properties, including the evolution and final composition of the fermenting microbiota. In the present work the effects of partially replacing NaCl with KCl in fermenting brines on the microbiological quality of Nocellara del Belice olives produced by Spanish style (Sivigliano) or Castelvetrano methods have been analyzed...
May 2017: Food Microbiology
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