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Lab quality

L J Zeng, W W Yang, P Tie, X R Liu, X R Gao, Z Y Li, P Hou, Y Zhi, Y F Bai, M J Geng, Q L Chen, B Y Cui, Z J Li, L P Wang
Objective: To evaluate the accuracy of human brucellosis diagnosis and reporting in medical institutions in Shanxi province, and understand the performance of clinical doctors to diagnose human brucellosis according to diagnostic criteria. Methods: Field investigation was conducted in 6 medical institutions in the key areas of human brucellosis in Shanxi province. The diagnosis data of the reported brucellosis cases in 2015 were collected and reviewed retrospectively for the evaluation of the diagnosis accuracy with systematic sampling method...
November 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
Libin Jia, Hongsheng Lin, Joost Oppenheim, O M Zack Howard, Jie Li, Huiting Fan, Zhizheng Zhao, William Farrar, Ying Zhang, Nancy Colburn, Matthew R Young, Weidong Li, David Newman, Barry R O'Keefe, John Beutler, Jikai Liu, Xiaojiang Hao, Xiaosheng Yang, Tengfei Ji, Jeffrey D White
Since 2007, the US National Cancer Institute (NCI) Office of Cancer Complementary and Alternative Medicine (OCCAM), together with the Cancer Institute of the China Academy of Chinese Medical Sciences (CICACMS), institutes at China Academy of Sciences and Chinese Academy of Medical Sciences, have engaged in collaborations on Chinese medicine (CM) and cancer research. Through these collaborations, CM drugs and compounds have been studied at NCI labs. This paper summarizes the discoveries and progress on these research projects, exploring the aspects of cancer prevention, botanical drug mechanisms of action and component analysis/quality control (QC), and anticancer activity screening...
November 1, 2017: Journal of the National Cancer Institute. Monographs
Assar Ali Shah, Yuan Xianjun, Dong Zhihao, Li Junfeng, Tao Sao
The study was conducted to evaluate the microbiological and chemical profiles of elephant grass inoculated with and without different wild strains of lactic acid bacteria. Silage was prepared of four treatments and one control with three replicates as control (EKC, adding 2 ml/kg sterilizing water), Lactobacillus plantarum (USA commercial bacteria) (EKP), Lactobacillus plantarum (EKA), Pediococcus acidilactici (EKB), and Pediococcus acidilactici (SKD) isolated from King grass. Silage were prepared using polyethylene terephthalate bottles, and incubated at room temperature for different ensiling days...
November 11, 2017: Archives of Microbiology
Ronghua Liu, Qiaofeng Sun, Tian Hu, Lian Li, Lei Nie, Jiayue Wang, Wanhui Zhou, Hengchang Zang
As a powerful process analytical technology (PAT) tool, near infrared (NIR) spectroscopy has been widely used in real-time monitoring. In this study, NIR spectroscopy was applied to monitor multi-parameters of traditional Chinese medicine (TCM) Shenzhiling oral liquid during the concentration process to guarantee the quality of products. Five lab scale batches were employed to construct quantitative models to determine five chemical ingredients and physical change (samples density) during concentration process...
October 28, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Dorota H Sendorek, Emilie Lalonde, Cindy Q Yao, Veronica Y Sabelnykova, Robert G Bristow, Paul C Boutros
Summary: The NanoString System is a well-established technology for measuring RNA and DNA abundance. Although it can estimate copy number variation, relatively few tools support analysis of these data. To address this gap, we created NanoStringNormCNV, an R package for pre-processing and copy number variant calling from NanoString data. This package implements algorithms for pre-processing, quality-control, normalization and copy number variation detection. A series of reporting and data visualization methods support exploratory analyses...
November 3, 2017: Bioinformatics
Ann Saterbak, Anoosha Moturu, Tracy Volz
Rice University's bioengineering department incorporates written, oral, and visual communication instruction into its undergraduate curriculum to aid student learning and to prepare students to communicate their knowledge and discoveries precisely and persuasively. In a tissue culture lab course, we used a self- and peer-review tool called Calibrated Peer Review™ (CPR) to diagnose student learning gaps in visual communication skills on a poster assignment. We then designed an active learning intervention that required students to practice the visual communication skills that needed improvement and used CPR to measure the changes...
November 3, 2017: Annals of Biomedical Engineering
Berthold Boedeker, Adam Goldstein, Ekta Mahajan
The availability and use of pre-sterilized disposables has greatly changed the methods used in biopharmaceuticals development and production, particularly from mammalian cell culture. Nowadays, almost all process steps from cell expansion, fermentation, cell removal, and purification to formulation and storage of drug substances can be carried out in disposables, although there are still limitations with single-use technologies, particularly in the areas of pretesting and quality control of disposables, bag and connections standardization and qualification, extractables and leachables (E/L) validation, and dependency on individual vendors...
November 4, 2017: Advances in Biochemical Engineering/biotechnology
Laura D Hughes, Scott A Lewis, Michael E Hughes
RNA-sequencing (RNA-seq) and microarrays are methods for measuring gene expression across the entire transcriptome. Recent advances have made these techniques practical and affordable for essentially any laboratory with experience in molecular biology. A variety of computational methods have been developed to decrease the amount of bioinformatics expertise necessary to analyze these data. Nevertheless, many barriers persist which discourage new labs from using functional genomics approaches. Since high-quality gene expression studies have enduring value as resources to the entire research community, it is of particular importance that small labs have the capacity to share their analyzed datasets with the research community...
2017: PloS One
Kai Jin, Mei Zhou, Shaoze Wang, Lixia Lou, Yufeng Xu, Juan Ye, Dahong Qian
PURPOSE: Retinal imaging is an important and effective tool for detecting retinal diseases. However, degraded images caused by the aberrations of the eye can disguise lesions, so that a diseased eye can be mistakenly diagnosed as normal. In this work, we propose a new image enhancement method to improve the quality of degraded images. METHODS: A new method is used to enhance degraded-quality fundus images. In this method, the image is converted from the input RGB colour space to LAB colour space and then each normalized component is enhanced using contrast-limited adaptive histogram equalization...
November 1, 2017: Acta Ophthalmologica
Amgad N Makaryus, Haisam Ismail, John N Makaryus, Dali Fan
AIM: To apply real time three-dimensional transesophageal echocardiography (RT3D TEE) for quantitative and qualitative assessment of the mitral valve annulus (MVA) and tricuspid valve annulus (TVA) in the same patient. METHODS: Our retrospective cohort study examined the MVA and TVA in 49 patients by RT3D TEE. MVA and TVA shape were examined by TEE. The MVA and TVA volume data set images were acquired in the mid esophageal 4-chamber view. The MVA and TVA were acquired separately, with optimization of each for the highest frame rate and image quality...
September 26, 2017: World Journal of Cardiology
Sangdon Ryu, Mi Ri Park, Brighton E Maburutse, Su Jin Lee, Woong Ji Lee, Soohyun Cho, Inho Hwang, Sangnam Oh, Younghoon Kim
Beef was dry aged for 40 to 60 days under controlled environmental conditions in a refrigerated room with a relative humidity of 75% to 80 % and air-flow. To date, there is little information on the microbial diversity and characteristics of dry aged beef. Here, we explored the effect of change in meat microorganisms on dry aged beef. Initially, total bacteria and LAB were significantly increased for 50 days during all dry aging periods. There was absence of representative foodborne pathogens as well as coliforms...
October 30, 2017: Journal of Microbiology and Biotechnology
Xu Hua, Rongjun Tang, Xiuxiu Xu, Zhi Wang, Qi Xu, Luxiao Chen, Edgar Wingender, Jie Li, Chenyu Zhang, Jin Wang
The cell-specific information of transcriptional regulation on microRNAs (miRNAs) is crucial to the precise understanding of gene regulations in various physiological and pathological processes existed in different tissues and cell types. The database, mirTrans, provides comprehensive information about cell-specific transcription of miRNAs including the transcriptional start sites (TSSs) of miRNAs, transcription factor (TF) to miRNA regulations and miRNA promoter sequences. mirTrans also maps the experimental H3K4me3 and DHS (DNase-I hypersensitive site) marks within miRNA promoters and expressed sequence tags (ESTs) within transcribed regions...
October 25, 2017: Nucleic Acids Research
Rui Fang, Kazunari Tanaka, Vamsi Mudhivarthi, Robin H Bogner, Michael J Pikal
Several controlled ice nucleation techniques have been developed to increase the efficiency of the freeze-drying process as well as to improve the quality of pharmaceutical products. Due to the reduction in ice surface area, these techniques have the potential to reduce the degradation of proteins labile during freezing. The objective of this study was to evaluate the effect of ice nucleation temperature on the in-process stability of lactate dehydrogenase (LDH). LDH in potassium phosphate buffer was nucleated at -4°C, -8°C, and -12°C using ControLyo(™) or allowed to nucleate spontaneously...
October 23, 2017: Journal of Pharmaceutical Sciences
Mark J Travers, Jessica A Kulak, Lisa Vogl
INTRODUCTION: The use of a waterpipe to smoke tobacco has emerged as a popular trend in the United States. Waterpipe smoking establishments have had an increasing presence in the U.S., despite smoke-free air legislation. Dangers of waterpipe smoking have been documented, but less data has been gathered about the waterpipe café itself. This project sought to determine a waterpipe-specific calibration factor (CF) for measuring waterpipe aerosol, and field-test this CF by conducting surveillance on the existing waterpipe cafés of western and central New York...
October 12, 2017: International Journal of Hygiene and Environmental Health
Polina Galitskaya, Liliya Biktasheva, Anatoly Saveliev, Tatiana Grigoryeva, Eugenia Boulygina, Svetlana Selivanovskaya
Composting is viewed as one of the primary methods to treat organic wastes. Co-composting may improve the efficiency of this treatment by establishing the most suitable conditions for decomposers than those present in the individual wastes. Given that bacteria and fungi are the driving agents of composting, information about the composition of their communities and dynamics during composting may improve reproducibility, performance and quality of the final compost as well as help to evaluate the potential human health risk and the choice of the most appropriate application procedure...
2017: PloS One
Evan R Coffey, Didier Muvandimwe, Yolanda Hagar, Christine Wiedinmyer, Ernest Kanyomse, Ricardo Piedrahita, Katherine L Dickinson, Abraham Oduro, Michael P Hannigan
Household cooking using solid biomass fuels is a major global health and environmental concern. As part of the Research on Emissions Air quality Climate and Cooking Technologies in Northern Ghana study, we conducted 75 in-field uncontrolled cooking tests designed to assess emissions and efficiency of the Gyapa woodstove, Philips HD4012, threestone fire and coalpot (local charcoal stove). Emission factors (EFs) were calculated for carbon monoxide (CO), carbon dioxide (CO2), and particulate matter (PM). Moreover, modified combustion (MCE), heat transfer (HTE) and overall thermal efficiencies (OTE) were calculated across a variety of fuel, stove and meal type combinations...
November 7, 2017: Environmental Science & Technology
Zhenshang Xu, Huiying He, Susu Zhang, Jian Kong
To improve silage quality of crop forages, bacterial inoculants are often employed. In this study, Lactobacillus brevis SDMCC050297 and Lactobacillus parafarraginis SDMCC050300 were used as inoculants to corn stover in lab silos for ensiling. At the initial stage of ensiling, the pH value of the inoculated silages reduced more drastically, and the inoculated silages had higher lactic acid and acetic acid contents. After 20 days of ensiling, a reduction in lactic acid content coupled with an increase in acetic acid and 1,2-propanediol contents was observed in inoculated silages...
October 19, 2017: Scientific Reports
Fujun Sun, Zhongyuan Fu, Chunhong Wang, Zhaoxiang Ding, Chao Wang, Huiping Tian
We propose and investigate an ultra-compact air-mode photonic crystal nanobeam cavity (PCNC) with an ultra-high quality factor-to-mode volume ratio (Q/V) by quadratically tapering the lattice space of the rectangular holes from the center to both ends while other parameters remain unchanged. By using the three-dimensional finite-difference time-domain method, an optimized geometry yields a Q of 7.2×10(6) and a V∼1.095(λ/nSi)(3) in simulations, resulting in an ultra-high Q/V ratio of about 6.5×10(6)(λ/nSi)(-3)...
May 20, 2017: Applied Optics
Maurizio Galderisi, Bernard Cosyns, Thor Edvardsen, Nuno Cardim, Victoria Delgado, Giovanni Di Salvo, Erwan Donal, Leyla Elif Sade, Laura Ernande, Madalina Garbi, Julia Grapsa, Andreas Hagendorff, Otto Kamp, Julien Magne, Ciro Santoro, Alexandros Stefanidis, Patrizio Lancellotti, Bogdan Popescu, Gilbert Habib
Aims: This European Association Cardiovascular Imaging (EACVI) Expert Consensus document aims at defining the main quantitative information on cardiac structure and function that needs to be included in standard echocardiographic report following recent ASE/EACVI chamber quantification, diastolic function, and heart valve disease recommendations. The document focuses on general reporting and specific pathological conditions such as heart failure, coronary artery and valvular heart disease, cardiomyopathies, and systemic diseases...
October 17, 2017: European Heart Journal Cardiovascular Imaging
Xianqiang He, Yan Bai, Jun Wei, Jing Ding, Palanisamy Shanmugam, Difeng Wang, Qingjun Song, Xiaoxian Huang
The Moderate-resolution Wide-wavelengths Imager (MWI) is the ocean color sensor onboard the Chinese Tiangong-2 Space Lab, which was launched on Sept. 15, 2016. The MWI is also an experimental satellite sensor for the Chinese next generation ocean color satellites, HY-1E and HY-1F, which are scheduled for launch around 2021. With 100m spatial resolution and 18 bands in the visible light and infrared wavelengths, MWI provides high quality ocean color observations especially over coastal and inland waters. For the first time, this study presents some important results on water color products generated from the MWI for the oceanic and inland waters...
October 2, 2017: Optics Express
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