keyword
MENU ▼
Read by QxMD icon Read
search

Lab quality

keyword
https://www.readbyqxmd.com/read/29325818/simultaneous-determination-of-voriconazole-posaconazole-itraconazole-and-hydroxy-itraconazole-in-human-plasma-using-lcms-ms
#1
Nicole Jenkins, Marion Black, Hans G Schneider
INTRODUCTION: Invasive fungal infections are an increasing cause of mortality and morbidity in high risk patient populations such as those on immunosuppressive therapy. Triazole antifungals are recommended for the prevention and treatment of such infections. The aim of this study was to develop and validate a simple, sensitive and robust LCMS/MS method for the simultaneous analysis in human plasma of three frequently used antifungal drugs: voriconazole, posaconazole, and itraconazole...
January 8, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29322630/inter-laboratory-variability-for-the-measurement-of-direct-oral-anticoagulants-results-from-the-external-quality-assessment-scheme
#2
Armando Tripodi, Veena Chantarangkul, Cristina Legnani, Sophie Testa, Alberto Tosetto
BACKGROUND: Tests for direct oral anticoagulants (DOAC) are not widely available. The perception that they are difficult to be run and are subjected to large between-laboratory variation makes their implementation difficult. AIMS: We carried out proficiency-testing surveys for DOAC within the activity of the external quality-assessment scheme of the Italian Federation of Thrombosis Centers. DESIGN: Participants were provided with coded freeze-dried plasmas without or with graded concentrations of the three main DOAC and asked to measure PT, APTT, thrombin time and DOAC concentrations by dedicated tests...
January 10, 2018: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/29318340/evaluating-protocols-for-porcine-faecal-microbiome-recollection-storage-and-dna-extraction-from-the-farm-to-the-lab
#3
Anixa Muiños-Bühl, Oscar González-Recio, María Muñoz, Cristina Óvilo, Juan García-Casco, Ana I Fernández
There is a growing interest in understanding the role of the gut microbiome on productive and meat quality-related traits in livestock species in order to develop new useful tools for improving pig production systems and industry. Faecal samples are analysed as a proxy of gut microbiota and here the selection of suitable protocols for faecal sampling and DNA isolation is a critical first step in order to obtain reliable results, even more to compare results obtained from different studies. The aim of the current study was to establish in a cost-effective way, using automated ribosomal intergenic spacer analysis technique, a protocol for porcine faecal sampling and storage at farm and slaughterhouse and to determine the most efficient microbiota DNA isolation kit among those most widely used...
January 9, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29299664/trends-in-urinary-diversion-after-radical-cystectomy-for-urothelial-carcinoma
#4
Kinan Bachour, Izak Faiena, Amirali Salmasi, Andrew T Lenis, David C Johnson, Aydin Pooli, Alexandra Drakaki, Allan J Pantuck, Karim Chamie
PURPOSE: To assess how trends in urinary diversion (UD) type following radical cystectomy (RC) have changed in recent years and investigate pre-operative predictors of UD type. METHODS: Data were abstracted from the American College of Surgeons National Surgical Quality Improvement Program (ACS NSQIP) from 2011 to 2015. We quantified the percentages of continent diversions (CD) versus incontinent diversions (ID) completed over this time frame. Using univariate and multivariable logistic regression analyses, we compared UD type across year of operation as well as predictors of type of diversion...
January 3, 2018: World Journal of Urology
https://www.readbyqxmd.com/read/29289775/meltwater-from-snow-contaminated-by-oil-sands-emissions-is-toxic-to-larval-fish-but-not-spring-river-water
#5
J L Parrott, J R Marentette, L M Hewitt, M E McMaster, P L Gillis, W P Norwood, J L Kirk, K M Peru, J V Headley, Z Wang, C Yang, R A Frank
To assess the toxicity of winter-time atmospheric deposition in the oil sands mining area of Northern Alberta, embryo-larval fathead minnow (Pimephales promelas) were exposed to snowmelt samples. Snow was collected in 2011-2014 near (<7km) oil sands open pit mining operations in the Athabasca River watershed and at sites far from (>25km) oil sands mining. Snow was shipped frozen back to the laboratory, melted, and amended with essential ions prior to testing. Fertilized fathead minnow eggs were exposed (<24h post-fertilization to 7-16days post-hatch) to a range of 25%-100% snowmelt...
December 28, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29288907/effect-of-pomegranate-based-marinades-on-the-microbiological-chemical-and-sensory-quality-of-chicken-meat-a-metabolomics-approach
#6
Anastasia E Lytou, George-John E Nychas, Efstathios Z Panagou
Pomegranate juice is a product with enhanced functional properties that could be used as an alternative to traditional marination ingredients and effectively retard microbial growth along with providing an improved sensory result. In this study, two pomegranate based marinades were prepared for the marination of chicken breast fillets and the marinated samples were aerobically stored at 4 and 10°C for 9days. Raw, non-marinated chicken samples were used as control. Levels of total viable counts (TVC), Pseudomonas spp...
December 21, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29286407/generic-protocol-for-optimization-of-heterologous-protein-production-using-automated-microbioreactor-technology
#7
Johannes Hemmerich, Lars Freier, Wolfgang Wiechert, Eric von Lieres, Marco Oldiges
A core business in industrial biotechnology using microbial production cell factories is the iterative process of strain engineering and optimization of bioprocess conditions. One important aspect is the improvement of cultivation medium to provide an optimal environment for microbial formation of the product of interest. It is well accepted that the media composition can dramatically influence overall bioprocess performance. Nutrition medium optimization is known to improve recombinant protein production with microbial systems and thus, this is a rewarding step in bioprocess development...
December 15, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29284083/ecotoxicology-goes-on-a-chip-embracing-miniaturized-bioanalysis-in-aquatic-risk-assessment
#8
Olivia Campana, Donald Wlodkowic
Biological and environmental sciences are, more than ever, becoming highly dependent on technological and multidisciplinary approaches that warrant advanced analytical capabilities. Microfluidic Lab-on-a-Chip technologies are perhaps one the most groundbreaking offshoots of bioengineering, enabling design of an entirely new generation of bioanalytical instrumentation. They represent a unique approach to combine micro-scale engineering and physics with specific biological questions, providing technological advances that allow for fundamentally new capabilities in the spatio-temporal analysis of molecules, cells, tissues and even small metazoan organisms...
December 28, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29277079/relationships-between-dbp-concentrations-and-differential-uv-absorbance-in-full-scale-conditions
#9
Nicolas Beauchamp, Olivier Laflamme, Sabrina Simard, Caetano Dorea, Geneviève Pelletier, Christian Bouchard, Manuel Rodriguez
Differential UV spectroscopy, defined as the difference in UV absorbance spectra before and after chlorination, has shown great potential to predict disinfection by-product (DBP) concentrations at laboratory scale. However, so far, no results have been reported on the full scale application of differential UV spectroscopy in drinking water treatment facilities. The objectives of this study are to determine if relationships can be developed between differential UV absorbance and DBP concentrations, for both regulated and unregulated DBPs, in a full-scale facility and to determine if these relationships vary throughout the year with variations in raw water quality and treatment conditions...
December 18, 2017: Water Research
https://www.readbyqxmd.com/read/29273087/comparison-of-multiple-transcriptomes-exposes-unified-and-divergent-features-of-quiescent-and-activated-skeletal-muscle-stem-cells
#10
Natalia Pietrosemoli, Sébastien Mella, Siham Yennek, Meryem B Baghdadi, Hiroshi Sakai, Ramkumar Sambasivan, Francesca Pala, Daniela Di Girolamo, Shahragim Tajbakhsh
BACKGROUND: Skeletal muscle satellite (stem) cells are quiescent in adult mice and can undergo multiple rounds of proliferation and self-renewal following muscle injury. Several labs have profiled transcripts of myogenic cells during the developmental and adult myogenesis with the aim of identifying quiescent markers. Here, we focused on the quiescent cell state and generated new transcriptome profiles that include subfractionations of adult satellite cell populations, and an artificially induced prenatal quiescent state, to identify core signatures for quiescent and proliferating...
December 22, 2017: Skeletal Muscle
https://www.readbyqxmd.com/read/29248213/effect-of-leaving-milk-trucks-empty-and-idle-for-6-h-between-raw-milk-loads
#11
Eva Kuhn, Lisbeth Meunier-Goddik, Joy G Waite-Cusic
The US Pasteurized Milk Ordinance (PMO) allows milk tanker trucks to be used repeatedly for 24 h before mandatory clean-in-place cleaning, but no specifications are given for the length of time a tanker can be empty between loads. We defined a worst-case hauling scenario as a hauling vessel left empty and dirty (idle) for extended periods between loads, especially in warm weather. Initial studies were conducted using 5-gallon milk cans (pilot-scale) as a proof-of-concept and to demonstrate that extended idle time intervals could contribute to compromised raw milk quality...
December 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29247916/studies-on-influence-of-process-parameters-on-simultaneous-biodegradation-of-atrazine-and-nutrients-in-aquatic-environments-by-a-membrane-photobioreactor
#12
Zahra Derakhshan, Amir Hossein Mahvi, Mohammad Hassan Ehrampoush, Seyed Mohammad Mazloomi, Mohammad Faramarzian, Mansooreh Dehghani, Saeed Yousefinejad, Mohammad Taghi Ghaneian, S Mehran Abtahi
A Lab scale algal-bacterial membrane photobioreactor (MPBR) was designed and operated under 12-h light and 12-h dark conditions with a light intensity of 8000lx, in order to investigate the effects of initial concentrations of atrazine, carbon concentration, and hydraulic retention time on the ability of this photobioreactor in simultaneous removal of atrazine and nutrients in the continuous mode. The removal efficiencies of atrazine (ATZ), chemical oxygen demand (COD), phosphorus (PO43--P) and nitrogen (NOx) in optimum condition was more than 95%, 99%, 98% and 97% when the maximum removal rates were 9...
December 13, 2017: Environmental Research
https://www.readbyqxmd.com/read/29244014/camsa-a-tool-for-comparative-analysis-and-merging-of-scaffold-assemblies
#13
Sergey S Aganezov, Max A Alekseyev
BACKGROUND: Despite the recent progress in genome sequencing and assembly, many of the currently available assembled genomes come in a draft form. Such draft genomes consist of a large number of genomic fragments (scaffolds), whose positions and orientations along the genome are unknown. While there exists a number of methods for reconstruction of the genome from its scaffolds, utilizing various computational and wet-lab techniques, they often can produce only partial error-prone scaffold assemblies...
December 6, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/29242636/a-millifluidic-system-for-analysis-of-daphnia-magna-locomotory-responses-to-water-born-toxicants
#14
Yushi Huang, Olivia Campana, Donald Wlodkowic
Aquatic toxicity testing in environmental monitoring and chemical risk assessment is critical to assess water quality for human use as well as predict impact of pollutants on ecosystems. In recent years, studies have increasingly focused on the relevance of sub-lethal effects of environmental contaminants. Sub-lethal toxicity endpoints such as behavioural responses are highly integrative and have distinct benefits for assessing water quality because they occur rapidly and thus can be used to sense the presence of toxicants...
December 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29239306/in-plane-x-ray-diffraction-for-characterization-of-monolayer-and-few-layer-transition-metal-dichalcogenides-films
#15
Mikhail Chubarov, Tanushree H Choudhury, Xiaotian Zhang, Joan M Redwing
There is significant interest in the growth of single crystal monolayer and few-layer films of transition metal dichalcogenides (TMD) and other 2D materials for scientific exploration and potential applications in optics, electronics, sensing, catalysis and others. The characterization of these materials is crucial in determining the properties and hence the applications. The ultra-thin nature of 2D layers presents a challenge to the use of X-ray diffraction (XRD) analysis with conventional Bragg-Brentano geometry in analyzing the crystallinity and epitaxial orientation of 2D films...
December 14, 2017: Nanotechnology
https://www.readbyqxmd.com/read/29226448/better-measurement-for-performance-improvement-in-low-and-middle-income-countries-the-primary-health-care-performance-initiative-phcpi-experience-of-conceptual-framework-development-and-indicator-selection
#16
Jeremy Veillard, Krycia Cowling, Asaf Bitton, Hannah Ratcliffe, Meredith Kimball, Shannon Barkley, Laure Mercereau, Ethan Wong, Chelsea Taylor, Lisa R Hirschhorn, Hong Wang
Policy Points: Strengthening accountability through better measurement and reporting is vital to ensure progress in improving quality primary health care (PHC) systems and achieving universal health coverage (UHC). The Primary Health Care Performance Initiative (PHCPI) provides national decision makers and global stakeholders with opportunities to benchmark and accelerate performance improvement through better performance measurement. Results from the initial PHC performance assessments in low- and middle-income countries (LMICs) are helping guide PHC reforms and investments and improve the PHCPI's instruments and indicators...
December 2017: Milbank Quarterly
https://www.readbyqxmd.com/read/29222941/characterization-of-the-spoilage-potential-of-pure-and-mixed-cultures-of-bacterial-species-isolated-from-tropical-yellowfin-tuna-thunnus-albacares
#17
Adèle Silbande, Josiane Cornet, Mireille Cardinal, Frédérique Chevalier, Katia Rochefort, Juliette Smith-Ravin, Sandra Adenet, Françoise Leroi
AIM: The spoilage potential of 28 bacterial strains isolated from spoiled raw yellowfin tuna was evaluated. METHODS AND RESULTS: Bacterial species were inoculated in irradiated tuna matrix. Chemical changes, bacterial growth and sensory quality were monitored during aerobic storage at 8°C. Pseudomonas spp., Enterobacter spp. and Escherichia hermanii had no spoiling effect. Brochothrix thermosphacta and Carnobacterium divergens/maltaromaticum developed moderate unpleasant odors...
December 9, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29217152/advanced-cardiopulmonary-resuscitation-cpr-in-the-catheterization-laboratory-consensus-document-of-the-working-groups-of-1-cardiopulmonary-resuscitation-acute-cardiac-care-and-2-hemodynamic-and-interventional-cardiology-hellenic-cardiological-society
#18
George Latsios, George Mpompotis, Kostas Tsioufis, Kostas Toutouzas, Emmanouil Skalidis, Andreas Synetos, Dimosthenis Avramidis, Dimitris Tousoulis
The incidence of Cardiac (Cardio-Pulmonary) Arrest (CA) in the Catheterization Laboratory is not common. However, not only does it happen occasionally but its frequency tends to increase due to the increase in the overall number and complexity of interventions performed by interventional cardiologists. Therefore, certified knowledge and application of the ALS (Advanced Life Support) protocols by trained Catheterization Laboratory staff is imperative in many countries, by local Hospital standards and also by Law...
December 4, 2017: Hellenic Journal of Cardiology: HJC, Hellēnikē Kardiologikē Epitheōrēsē
https://www.readbyqxmd.com/read/29215764/an-evaluation-of-the-challenges-to-developing-tumour-brca1-and-brca2-testing-methodologies-for-clinical-practice
#19
Gillian Ellison, Miika Ahdesmäki, Sally Luke, Paul M Waring, Andrew Wallace, Ronnie Wright, Benno Röthlisberger, Katja Ludin, Sabine Merkelbach-Bruse, Carina Heydt, Marjolijn J L Ligtenberg, Arjen R Mensenkamp, David Gonzalez Castro, Thomas Jones, Ana Vivancos, Olga Kondrashova, Patrick Pauwels, Christine Weyn, Eric Hahnen, Jan Hauke, Richie Soong, Zhongwu Lai, Brian Dougherty, T Hedley Carr, Justin Johnson, John Mills, J Carl Barrett
Ovarian cancer patients with germline or somatic pathogenic variants benefit from treatment with PARP inhibitors. Tumour BRCA1/2 testing is more challenging than germline testing as the majority of samples are formalin fixed paraffin embedded (FFPE), the tumour genome is complex and the allelic fraction of somatic variants can be low. We collaborated with 10 laboratories testing BRCA1/2 in tumours to compare different approaches to identify clinically important variants within FFPE tumour DNA samples. This was not a proficiency study but an inter-laboratory comparison to identify common issues...
December 7, 2017: Human Mutation
https://www.readbyqxmd.com/read/29210813/effect-of-low-volume-split-dose-purgative-on-the-quality-of-bowel-prep-for-colonoscopy-on-the-hospitalized-patient
#20
Judy A Corliss
Although colon cancer is preventable, it is still the second leading cause of cancer death in both men and women in the United States (). Colonoscopy is as yet the gold standard for colon cancer prevention (). The success of colonoscopy is dependent upon the quality of the bowel prep. The problem addressed in this study was the impact of hospitalization on bowel prep quality. The participants in this study were 54 consecutive inpatients older than 18 years and 52 consecutive outpatients older than 18 years who were scheduled for colonoscopy in the Endoscopy Lab at Santa Barbara Cottage Hospital...
November 2017: Gastroenterology Nursing: the Official Journal of the Society of Gastroenterology Nurses and Associates
keyword
keyword
55286
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"