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https://www.readbyqxmd.com/read/28324615/construction-of-low-ethanol-wine-yeasts-through-partial-deletion-of-the-saccharomyces-cerevisiae-pdc2-gene
#1
Raúl Andrés Cuello, Karina Johana Flores Montero, Laura Analía Mercado, Mariana Combina, Iván Francisco Ciklic
We propose an alternative GMO based strategy to obtain Saccharomyces cerevisiae mutant strains with a slight reduction in their ability to produce ethanol, but with a moderate impact on the yeast metabolism. Through homologous recombination, two truncated Pdc2p proteins Pdc2pΔ344 and Pdc2pΔ519 were obtained and transformed into haploid and diploid lab yeast strains. In the pdc2Δ344 mutants the DNA-binding and transactivation site of the protein remain intact, whereas in pdc2Δ519 only the DNA-binding site is conserved...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28318928/structured-evaluation-and-need-based-restructuring-of-the-cranio-maxillofacial-surgery-module-within-surgical-clerkship
#2
Sebastian Herbert Hoefer, Jasmina Sterz, Bernd Bender, Christina Stefanescu, Marius Theis, Felix Walcher, Robert Sader, Miriam Ruesseler
BACKGROUND: Evaluations are important for teaching courses and contribute to educational quality assurance. CMF surgery provides a module in the skills-lab week in preparation for surgical clerkship. Even though the CMF module receives positive evaluations, the students report deviating content. Subsequently, exams skills were often not mastered correctly. The aim of this study is to gather the contents taught within the course and to revise the module accordingly. METHODS: A structured evaluation sheet was used to evaluate the CMF modules...
February 13, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/28318695/monitoring-water-quality-in-toronto-s-urban-stormwater-ponds-assessing-participation-rates-and-data-quality-of-water-sampling-by-citizen-scientists-in-the-freshwater-watch
#3
Andrew B Scott, Paul C Frost
From 2013 to 2015, citizen scientist volunteers in Toronto, Canada were trained to collect and analyze water quality in urban stormwater ponds. This volunteer sampling was part of the research program, FreshWater Watch (FWW), which aimed to standardize urban water sampling efforts from around the globe. We held training sessions for new volunteers twice yearly and trained a total of 111 volunteers. Over the course of project, ~30% of volunteers participated by collecting water quality data after the training session with 124 individual sampling events at 29 unique locations in Toronto, Canada...
March 16, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28315210/recombinant-allergens-production-in-yeast
#4
Maria Neophytou, Marcos Alcocer
The methylotropic yeast Pichia pastoris has been extensively used in large-scale production of properly folded recombinant proteins. As an eukaryotic organism P. pastoris presents a series of advantages at expression and processing of heterologous proteins such as post-translational modifications, protein processing, and a reasonably sophisticated quality control of protein folding when compared against Escherichia coli. In this chapter, we describe the modified lab procedure for cloning and expression in Pichia pastoris of common food allergens sequences from the raw fruit to the fully folded biotinylated protein product...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28314413/recent-progress-in-the-discovery-and-development-of-trpa1-modulators
#5
S Skerratt
TRPA1 is a well-validated therapeutic target in areas of high unmet medical need that include pain and respiratory disorders. The human genetic rationale for TRPA1 as a pain target is provided by a study describing a rare gain-of-function mutation in TRPA1, causing familial episodic pain syndrome. There is a growing interest in the TRPA1 field, with many pharmaceutical companies reporting the discovery of TRPA1 chemical matter; however, GRC 17536 remains to date the only TRPA1 antagonist to have completed Phase IIa studies...
2017: Progress in Medicinal Chemistry
https://www.readbyqxmd.com/read/28284763/validation-of-a-two-dimensional-liquid-chromatography-method-for-quality-control-testing-of-pharmaceutical-materials
#6
Samuel H Yang, Jenny Wang, Kelly Zhang
Despite the advantages of 2D-LC, there is currently little to no work in demonstrating the suitability of these 2D-LC methods for use in a quality control (QC) environment for good manufacturing practice (GMP) tests. This lack of information becomes more critical as the availability of commercial 2D-LC instrumentation has significantly increased, and more testing facilities begin to acquire these 2D-LC capabilities. It is increasingly important that the transferability of developed 2D-LC methods be assessed in terms of reproducibility, robustness and performance across different laboratories worldwide...
March 1, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28284190/notifiable-condition-reporting-practices-implications-for-public-health-agency-participation-in-a-health-information-exchange
#7
Debra Revere, Rebecca H Hills, Brian E Dixon, P Joseph Gibson, Shaun J Grannis
BACKGROUND: The future of notifiable condition reporting in the United States is undergoing a transformation with the increasing development of Health Information Exchanges which support electronic data-sharing and -transfer networks and the wider adoption of electronic laboratory reporting. Communicable disease report forms originating in clinics are an important source of surveillance data for public health agencies. However, problems of poor data quality and delayed submission of reports to public health agencies are common...
March 11, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28279924/physicians-responses-to-financial-and-social-incentives-a-medically-framed-real-effort-experiment
#8
Mylène Lagarde, Duane Blaauw
Because compensation policies have critical implications for the provision of health care, and evidence of their effects is limited and difficult to study in the real world, laboratory experiments may be a valuable methodology to study the behavioural responses of health care providers. With this experiment undertaken in 2013, we add to this new literature by designing a new medically framed real effort task to test the effects of different remuneration schemes in a multi-tasking context. We assess the impact of different incentives on the quantity (productivity) and quality of outputs of 132 participants...
March 2, 2017: Social Science & Medicine
https://www.readbyqxmd.com/read/28276234/use-of-psychrotolerant-lactic-acid-bacteria-lactobacillus-spp-and-leuconostoc-spp-isolated-from-chinese-traditional-paocai-for-the-quality-improvement-of-paocai-products
#9
Aiping Liu, Xiaoyan Li, Biao Pu, Xiaolin Ao, Kang Zhou, Li He, Shujuan Chen, Shuliang Liu
To improve the quality of Chinese traditional Paocai, two psychrotolerant lactic acid bacteria (LAB) strains were isolated from Paocai, and the quality of Chinese Paocai product using these two strains as starter cultures was compared to a control sample fermented with aged brine at 10 °C. The results suggested that the physicochemical and sensory features of Paocai fermented with psychrotolerant LAB were more suitable for industrial applications. The nitrite content of Paocai fermented with psychrotolerant LAB was 1 mg/kg, which was significantly lower than that of the control Paocai (P < 0...
March 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28276115/a-novel-feedstuff-ensiling-of-cowpea-vigna-unguiculata-l-stover-and-apple-malus-domestica-borkh-mixtures-evaluation-of-the-nutritive-value-fermentation-quality-and-aerobic-stability
#10
Ederson Andrade, Alexandre Gonçalves, Ana Mendes-Ferreira, Valéria Silva, Victor Pinheiro, Miguel Rodrigues, Luis Ferreira
BACKGROUND: Agro-industrial by-products have low economical value as foods for human consumption however might represent a potential value as animal feedstuffs. This study evaluated a novel feedstuff, ensiled discarded apple (85%) and cowpea stover (15%) mixtures with two distinct ensiling periods (45 and 60 days) regarding the nutritive value, fermentation quality and aerobic stability. RESULTS: Generally, no differences (P > 0.05) were observed between ensiling periods for its nutritive value and fermentation characteristics...
March 8, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28255773/cell-lines-authentication-and-mycoplasma-detection-as-minimun-quality-control-of-cell-lines-in-biobanking
#11
C Corral-Vázquez, R Aguilar-Quesada, P Catalina, G Lucena-Aguilar, G Ligero, B Miranda, J A Carrillo-Ávila
Establishment of continuous cell lines from human normal and tumor tissues is an extended and useful methodology for molecular characterization of cancer pathophysiology and drug development in research laboratories. The exchange of these cell lines between different labs is a common practice that can compromise assays reliability due to contamination with microorganism such as mycoplasma or cells from different flasks that compromise experiment reproducibility and reliability. Great proportions of cell lines are contaminated with mycoplasma and/or are replaced by cells derived for a different origin during processing or distribution process...
March 2, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28253333/personality-traits-and-ego-network-dynamics
#12
Simone Centellegher, Eduardo López, Jari Saramäki, Bruno Lepri
Strong and supportive social relationships are fundamental to our well-being. However, there are costs to their maintenance, resulting in a trade-off between quality and quantity, a typical strategy being to put a lot of effort on a few high-intensity relationships while maintaining larger numbers of less close relationships. It has also been shown that there are persistent individual differences in this pattern; some individuals allocate their efforts more uniformly across their networks, while others strongly focus on their closest relationships...
2017: PloS One
https://www.readbyqxmd.com/read/28251763/a-13-week-research-based-biochemistry-laboratory-curriculum
#13
Scott T Lefurgy, Emily C Mundorff
Here, we present a 13-week research-based biochemistry laboratory curriculum designed to provide the students with the experience of engaging in original research while introducing foundational biochemistry laboratory techniques. The laboratory experience has been developed around the directed evolution of an enzyme chosen by the instructor, with mutations designed by the students. Ideal enzymes for this curriculum are able to be structurally modeled, solubly expressed, and monitored for activity by UV/Vis spectroscopy, and an example curriculum for haloalkane dehalogenase is given...
March 2, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28243925/utility-of-home-sleep-apnea-testing-in-high-risk-veterans
#14
Alyssa Cairns, Kathleen Sarmiento, Richard Bogan
PURPOSE: Many Veterans Affairs Medical Centers (VAMCs) have implemented home sleep apnea testing (HSAT) in lieu of traditional in-lab testing to establish a timely and cost-sensitive diagnosis of obstructive sleep apnea (OSA). However, concern remains for the sensitivity and specificity of said technology in this population as many veterans are at increased risk for many of the comorbid conditions that can limit the accuracy of HSAT results. Hence, the purpose of this study is to evaluate rate of incongruent outcomes (e...
February 27, 2017: Sleep & Breathing, Schlaf & Atmung
https://www.readbyqxmd.com/read/28240536/stratification-of-extracellular-polymeric-substances-eps-for-aggregated-anammox-microorganisms
#15
Fangxu Jia, Qing Yang, Xiuhong Liu, Xiyao Li, Baikun Li, Liang Zhang, Yongzhen Peng
Sludge aggregation and biofilm formation are the most effective approaches to solve the washout of anammox microorganisms. In this study, the structure and composition of EPS (extracellular polymeric substances) were investigated to elucidate the factors for the anammox aggregation property. Anammox sludge taken from 18 lab-scale and pilot-scale reactors treating different types of wastewater was analyzed using EEM-PARAFAC (excitation-emission matrix and parallel factor analysis), FTIR (Fourier transform infrared spectroscopy) and real-time PCR combined with multivariate statistical analysis...
March 21, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28226250/improved-performance-of-gravity-driven-membrane-filtration-for-seawater-pretreatment-implications-of-membrane-module-configuration
#16
Bing Wu, Tino Christen, Hwee Sin Tan, Florian Hochstrasser, Stanislaus Raditya Suwarno, Xin Liu, Tzyy Haur Chong, Michael Burkhardt, Wouter Pronk, Anthony G Fane
As a low energy and chemical free process, gravity-driven membrane (GDM) filtration has shown a potential for seawater pretreatment in our previous studies. In this study, a pilot submerged GDM reactor (effective volume of 720 L) was operated over 250 days and the permeate flux stabilized at 18.6 ± 1.4 L/m(2)h at a hydrostatic pressure of 40 mbar. This flux was higher than those in the lab-scale GDM reactor (16.3 ± 0.2 L/m(2)h; effective volume of 8.4 L) and in the filtration cell system (2.7 ± 0...
February 16, 2017: Water Research
https://www.readbyqxmd.com/read/28214447/influence-of-pressures-up-to-50bar-on-two-stage-anaerobic-digestion
#17
Wolfgang Merkle, Katharina Baer, Jonas Lindner, Simon Zielonka, Felix Ortloff, Frank Graf, Thomas Kolb, Thomas Jungbluth, Andreas Lemmer
The concept of pressurized two-stage anaerobic digestion integrates biogas production, purification and pressure boosting within one process. The produced methane-rich biogas can be fed into gas grids with considerably less purification effort. To investigate biogas production under high pressures up to 50bar, a lab scale two-stage anaerobic digestion system was constructed including one continuously operated pressurized methane reactor. This investigation examined the effects of different operating pressures in methane reactor (10, 25, 50bar) on biogas quantity and quality, pH value and process stability...
February 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28214369/diagnostic-accuracy-of-history-physical-exam-laboratory-tests-and-point-of-care-ultrasound-for-pediatric-acute-appendicitis-in-the-emergency-department-a-systematic-review-and-meta-analysis
#18
Roshanak Benabbas, Mark Hanna, Jay Shah, Richard Sinert
BACKGROUND: Acute appendicitis (AA) is the most common surgical emergency in children. Accurate and timely diagnosis is crucial but challenging due to atypical presentations and the inherent difficulty of obtaining a reliable history and physical examination in younger children. OBJECTIVES: To determine the utility of history, physical exam, laboratory tests, Pediatric Appendicitis Score (PAS) and Emergency Department-Point-of-Care Ultrasound (ED-POCUS) in the diagnosis of AA in ED pediatric patients...
February 18, 2017: Academic Emergency Medicine: Official Journal of the Society for Academic Emergency Medicine
https://www.readbyqxmd.com/read/28210467/diversity-and-distribution-of-culturable-lactic-acid-bacterial-species-in-indonesian-sayur-asin
#19
Wibowo Mangunwardoyo, Abinawanto, Andi Salamah, Endang Sukara, Sulistiani, Achmad Dinoto
BACKGROUND AND OBJECTIVES: Lactic acid bacteria (LAB) play important roles in processing of Sayur Asin (spontaneously fermented mustard). Unfortunately, information about LAB in Indonesian Sayur Asin, prepared by traditional manufactures which is important as baseline data for maintenance of food quality and safety, is unclear. The aim of this study was to describe the diversity and distribution of culturable lactic acid bacteria in Sayur Asin of Indonesia. MATERIALS AND METHODS: Four Sayur Asin samples (fermentation liquor and fermented mustard) were collected at harvesting times (3-7 days after fermentation) from two traditional manufactures in Tulung Agung (TA) and Kediri (KDR), East Java provinces, Indonesia...
August 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28209478/algal-bloom-response-and-risk-management-on-site-response-tools
#20
Susan B Watson, Arthur Zastepa, Gregory L Boyer, Eric Matthews
Harmful algal blooms caused by cyanobacteria can present a risk to the safety of drinking- and recreational waters and beachfronts through the production of toxins, particularly microcystin, which are highly resilient to degradation. These blooms are difficult to predict, vary in appearance and toxicity, and can show significant spatial heterogeneity: wind- and current-borne scums can produce an order of magnitude range in toxin levels along shorelines. The growing demand for reliable, cost-effective and rapid methods to detect toxins in bloom material and reduce the risk of public exposure cannot be met by most analytical lab turnaround times...
February 15, 2017: Toxicon: Official Journal of the International Society on Toxinology
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