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https://www.readbyqxmd.com/read/28738622/a-flow-based-procedure-exploiting-the-lab-in-syringe-approach-for-the-determination-of-ester-content-in-biodiesel-and-diesel-biodiesel-blends
#1
Samara Soares, Wanessa R Melchert, Fábio R P Rocha
The ester content is an important parameter to be monitored in biodiesel for evaluation of the transesterification reaction yield and for assessing the purity of the final product. This is also a relevant quality parameter in diesel/biodiesel blends to avoid frauds, because legislation establishes a minimum amount of biodiesel to be added to diesel. The official method EN14103 requires the addition of an alternative internal standard (methyl nonadecanoate) for analysis of biodiesel from bovine tallow because the methyl heptadecanoate is found in high amounts in this product...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738172/impact-of-polymeric-membrane-breakage-on-drinking-water-quality-and-an-online-detection-method-of-the-breakage
#2
Qilong Wu, Zhenghua Zhang, Guodong Cao, Xihui Zhang
Polymeric membrane has been widely used for the treatment of drinking water in China, and the total treating capacity has reached up to 3.8 million m(3)/d. However, the membrane breakage found in the membrane modules in many water treatment plants resulted in an increase in turbidity and bacterial amount in the membrane permeate. In this study, a membrane module running for 3 years in a full-scale application was examined in terms of the breaking positions and the numbers of the broken fibers. It was found that most of the breaking positions were mainly on the outlet side of the module and that the distance from these points to the outlet was about 1/10-2/10 length of the membrane module...
July 24, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28737080/sbr-treatment-of-tank-truck-cleaning-wastewater-sludge-characteristics-chemical-and-ecotoxicological-effluent-quality
#3
Michel Caluwé, Thomas Dobbeleers, Dominique Daens, Luc Geuens, Ronny Blust, Jan Dries
A lab scale activated sludge sequencing batch reactor (SBR) was used to treat tank truck cleaning (TTC) wastewater with different operational strategies (identified as different stages). The first stage was an adaptation period for the seed sludge that originated from a continuous fed industrial plant treating TTC wastewater. The first stage was followed by a dynamic reactor operation based on the oxygen uptake rate (OUR). Thirdly, dynamic SBR control based on OUR treated a daily changing influent. Lastly, the reactor was operated with a gradually shortened fixed cycle...
July 24, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28732554/microbial-ecology-and-process-technology-of-sourdough-fermentation
#4
Luc De Vuyst, Simon Van Kerrebroeck, Frédéric Leroy
From a microbiological perspective, sourdough is to be considered as a specific and stressful ecosystem, harboring yeasts and lactic acid bacteria (LAB), that is used for the production of baked goods. With respect to the metabolic impact of the sourdough microbiota, acidification (LAB), flavor formation (LAB and yeasts), and leavening (yeasts and heterofermentative LAB species) are most noticeable. Three distinct types of sourdough fermentation processes can be discerned based on the inocula applied, namely backslopped ones (type 1), those initiated with starter cultures (type 2), and those initiated with a starter culture followed by backslopping (type 3)...
2017: Advances in Applied Microbiology
https://www.readbyqxmd.com/read/28729170/how-do-pain-fatigue-depressive-and-cognitive-symptoms-relate-to-well-being-and-social-and-physical-functioning-in-the-daily-lives-of-individuals-with-multiple-sclerosis
#5
Anna L Kratz, Tiffany J Braley, Emily Foxen-Craft, Eric Scott, John Murphy, Susan L Murphy
OBJECTIVE: To examine the relative association between daily change in pain, fatigue, depressed mood, and cognitive function, and four outcomes (positive affect and well-being, ability to participate in social roles and activities, upper extremity (UE) functioning, and lower extremity (LE) functioning). DESIGN: Repeated-measures observational study including a baseline lab visit, followed by seven days of home monitoring that included ecological momentary assessment (EMA) of symptoms (pain, fatigue, depressed mood, and cognitive function) and end-of-day diary surveys of outcomes of interest...
July 17, 2017: Archives of Physical Medicine and Rehabilitation
https://www.readbyqxmd.com/read/28728399/fermentation-quality-and-in-vitro-methane-production-of-sorghum-silage-prepared-with-cellulase-and-lactic-acid-bacteria
#6
Waroon Khota, Suradej Pholsen, David Higgs, Cai Yimin
Objective: The effects of lactic acid bacteria (LAB) and cellulase enzyme on fermentation quality, microorganism population, chemical composition and in vitro gas production of sorghum silages were studied. Methods: Commercial inoculant Lactobacillus plantarum Chikuso 1 (CH), local selected strain L. casei TH 14 and Acremonium cellulase (AC) were used as additives in sorghum silage preparation. Results: Prior to ensiling Sorghum contained 104 LAB and 106 cfu/g fresh matter coliform bacteria...
May 14, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28725210/multicenter-analytical-validation-of-a%C3%AE-40-immunoassays
#7
Linda J C van Waalwijk van Doorn, Luka Kulic, Marleen J A Koel-Simmelink, H Bea Kuiperij, Alexandra A M Versleijen, Hanne Struyfs, Harry A M Twaalfhoven, Anthony Fourier, Sebastiaan Engelborghs, Armand Perret-Liaudet, Sylvain Lehmann, Marcel M Verbeek, Eugeen J M Vanmechelen, Charlotte E Teunissen
BACKGROUND: Before implementation in clinical practice, biomarker assays need to be thoroughly analytically validated. There is currently a strong interest in implementation of the ratio of amyloid-β peptide 1-42 and 1-40 (Aβ42/Aβ40) in clinical routine. Therefore, in this study, we compared the analytical performance of six assays detecting Aβ40 in cerebrospinal fluid (CSF) in six laboratories according to a recently standard operating procedure (SOP) developed for implementation of ELISA assays for clinical routine...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28724183/physiological-intra-cytoplasmic-sperm-injection-picsi-outcomes-after-oral-pretreatment-and-semen-incubation-with-myo-inositol-in-oligoasthenoteratozoospermic-men-results-from-a-prospective-randomized-controlled-trial
#8
T Korosi, C Barta, K Rokob, T Torok
OBJECTIVE: The aim of the current study was to evaluate the effect of an oral pretreatment with a mix of myo-inositol (Myo-Ins), folic acid, vitamin E, L-carnitine, L-arginine and selenium (Folandrol, Exeltis, Hungary) and subsequent direct Myo-Ins incubation of spermatozoa before Physiological Intra-Cytoplasmic Sperm Injection (PICSI) procedures in infertile couples due to oligoasthenoteratozoospermia with previous failed PICSI procedures. PATIENTS AND METHODS: We performed a prospective, randomized controlled trial at the Assisted Reproduction Unit of the Kaáli Institute (Győr, Hungary)...
June 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28720354/training-modalities-in-robot-assisted-urologic-surgery-a-systematic-review
#9
REVIEW
Catherine Elizabeth Lovegrove, Oussama Elhage, M Shamim Khan, Giacomo Novara, Alex Mottrie, Prokar Dasgupta, Kamran Ahmed
CONTEXT: Novel surgical techniques demand that surgical training adapts to the need for technical and nontechnical skills. OBJECTIVE: To identify training methods available for robot-assisted surgical (RAS) training in urology, evaluate their effectiveness in terms of validation, educational impact, acceptability, and cost effectiveness, and assess their effect on learning curves (LCs). EVIDENCE ACQUISITION: A systematic review following Preferred Reporting Items for Systematic Reviews and Meta-analysis guidelines searched Ovid Medline, Embase, PsycINFO, and the Cochrane Library...
February 2017: European Urology Focus
https://www.readbyqxmd.com/read/28714056/quality-control-in-diagnostic-immunohistochemistry-integrated-on-slide-positive-controls
#10
A Bragoni, A Gambella, S Pigozzi, M Grigolini, R Fiocca, L Mastracci, F Grillo
Standardization in immunohistochemistry is a priority in modern pathology and requires strict quality control. Cost containment has also become fundamental and auditing of all procedures must take into account both these principles. Positive controls must be routinely performed so that their positivity guarantees the appropriateness of the immunohistochemical procedure. The aim of this study is to develop a low cost (utilizing a punch biopsy-PB-tool) procedure to construct positive controls which can be integrated in the patient's tissue slide...
July 17, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28703952/potential-of-bacteriocins-from-lab-to-improve-microbial-quality-of-dry-cured-and-fermented-meat-products
#11
Paulina Kęska, Joanna Stadnik, Dorota Zielińska, Danuta Kołożyn-Krajewska
Meat and meat products are an important component of the daily diet. Nevertheless, they are perishable goods and are prone to microbial contamination, which leads to an increased risk to the health of consumers as well as economic losses in the meat industry. Fermentation has been used for thousands of years to preserve meat. As a result of extensive biochemical reactions occurring in meat during fermentation and ripening, the condi- tions inhibiting the growth of pathogenic and spoilage bacteria are formed...
April 2017: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28702463/supporting-quality-data-systems-lessons-learned-from-early-implementation-of-routine-viral-load-monitoring-at-a-large-clinic-in-lilongwe-malawi
#12
Jean Gibb, Jimmy Chitsulo, Chifundo Chipungu, Mackenzie Chivwara, Alan Schooley, Risa M Hoffman
Successful viral load programs rely on the presence of data systems and high quality of patient data. Using a cohort of 49 patients at Partners in Hope, a large, urban HIV clinic in Malawi, we performed a quality improvement assessment of a new viral load program with a focus on accuracy of data collected from patients as well as adherence to Malawi HIV Guidelines in regard to response to elevated viral loads (≥1,000 copies/mL). Data were obtained from three parallel medical record systems to investigate the proportion of patients with a repeat viral load and whether the three data systems agreed in regard to sociodemographic and clinical data...
2017: Journal of Clinical Research in HIV AIDS and Prevention
https://www.readbyqxmd.com/read/28696370/strategies-for-pathogen-biocontrol-using-lactic-acid-bacteria-and-their-metabolites-a-focus-on-meat-ecosystems-and-industrial-environments
#13
REVIEW
Patricia Castellano, Mariana Pérez Ibarreche, Mariana Blanco Massani, Cecilia Fontana, Graciela M Vignolo
The globalization of trade and lifestyle ensure that the factors responsible for the emergence of diseases are more present than ever. Despite biotechnology advancements, meat-based foods are still under scrutiny because of the presence of pathogens, which causes a loss of consumer confidence and consequently a fall in demand. In this context, Lactic Acid Bacteria (LAB) as GRAS organisms offer an alternative for developing pathogen-free foods, particularly avoiding Listeria monocytogenes, with minimal processing and fewer additives while maintaining the foods' sensorial characteristics...
July 11, 2017: Microorganisms
https://www.readbyqxmd.com/read/28696065/the-dynamics-of-the-bacterial-communities-developed-in-maize-silage
#14
Javad Gharechahi, Zohreh Akhavan Kharazian, Sajjad Sarikhan, Gholamreza Salehi Jouzani, Mahnaz Aghdasi, Ghasem Hosseini Salekdeh
Ensilage provides an effective means of conserving summer-grown green forage to supply as winter feed to ruminants. The fermentation process involved in the ensilage process relies on lactic acid bacteria (LAB). Here, 16S ribosomal DNA amplicon pyrosequencing was used to follow the dynamic behaviour of the LAB community during the ensilage of maize biomass, with a view to identify the key species involved in the process. The biomass used for ensilage was a single-cross maize hybrid, harvested at the milk-line stage...
July 11, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28695541/headphone-screening-to-facilitate-web-based-auditory-experiments
#15
Kevin J P Woods, Max H Siegel, James Traer, Josh H McDermott
Psychophysical experiments conducted remotely over the internet permit data collection from large numbers of participants but sacrifice control over sound presentation and therefore are not widely employed in hearing research. To help standardize online sound presentation, we introduce a brief psychophysical test for determining whether online experiment participants are wearing headphones. Listeners judge which of three pure tones is quietest, with one of the tones presented 180° out of phase across the stereo channels...
July 10, 2017: Attention, Perception & Psychophysics
https://www.readbyqxmd.com/read/28691347/design-and-operation-of-a-continuous-integrated-monoclonal-antibody-production-process
#16
Fabian Steinebach, Nicole Ulmer, Moritz Wolf, Lara Decker, Veronika Schneider, Ruben Wälchli, Daniel Karst, Jonathan Souquet, Massimo Morbidelli
The realization of an end-to-end integrated continuous lab-scale process for monoclonal antibody manufacturing is described. For this, a continuous cultivation with filter-based cell-retention, a continuous two column capture process, a virus inactivation step, a semi-continuous polishing step (twin-column MCSGP) and a batch-wise flow-through polishing step were integrated and operated together. In each unit the implementation of internal recycle loops allows to improve the performance: a) in the bioreactor, to simultaneously increase the cell density and volumetric productivity, b) in the capture process, to achieve improved capacity utilization at high productivity and yield and c) in the MCSGP process, to overcome the purity-yield trade-off of classical batch-wise bind-elute polishing steps...
July 10, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28677149/isolating-and-evaluating-lactic-acid-bacteria-strains-for-effectiveness-on-silage-quality-at-low-temperatures-on-the-tibetan-plateau
#17
REVIEW
Siran Wang, Xianjun Yuan, Zhihao Dong, Junfeng Li, Tao Shao
Four lactic acid bacteria (LAB) strains isolated from straw silages on the Tibetan Plateau were characterized, and their effects on the fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) at different temperatures (10°C, 15°C and 25°C) were studied. These LAB isolates were evaluated using the acids production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests. All the isolates (M1, LM8, LO7 and LOG9) could grow at 5-20°C, pH 3.5-7.0 and NaCl (3...
July 5, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28674926/enhancement-of-gamma-aminobutyric-acid-gaba-levels-using-an-autochthonous-lactobacillus-futsaii-cs3-as-starter-culture-in-thai-fermented-shrimp-kung-som
#18
Chatthaphisuth Sanchart, Onnicha Rattanaporn, Dietmar Haltrich, Pimpimol Phukpattaranont, Suppasil Maneerat
Gamma-aminobutyric acid (GABA) is a non-proteinogenic amino acid, which has a variety of well-characterized beneficial physiological functions. In order to improve GABA levels and the fermentation process of Thai fermented shrimp (Kung-Som), autochthonous Lactobacillus futsaii CS3 was inoculated as a starter culture into Kung-Som, and its effects on the quality of Kung-Som were studied. The optimal conditions for GABA production in Kung-Som as obtained by response surface methodology (RSM) using a central composite design (CCD) were an inoculum size of roughly 10(7) CFU/g (X1) of L...
August 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28669141/development-of-a-new-lactic-acid-bacterial-inoculant-for-fresh-rice-straw-silage
#19
Jong Geun Kim, Jun Sang Ham, Yu Wei Li, Hyung Soo Park, Chul-Sung Huh, Byung-Chul Park
OBJECTIVE: Effects of newly isolated Lactobacillus plantarum on the fermentation and chemical composition of fresh rice straw silage was evaluated in this study. METHODS: Lactic acid bacteria (LAB) from good crop silage were screened by growing them in MRS broth and a minimal medium with low carbohydrate content. Selected LAB (LAB 1821) were Gram-positive, rods, catalase negative, and were identified to be Lactobacillus plantarum based on their biochemical characteristics and a 16S rRNA analysis...
July 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28661361/automated-microscopy-and-high-content-screens-phenotypic-screens-in-academia-labs
#20
Dimitri Moreau, Jean Gruenberg
Imaged-based screening has been developing extremely quickly in the past 10 years. Academic institutes quickly realized that the discovery capacity of this technology was huge, allowing the automatic detection and quantification of complex cell phenotypes. Associated with chemical or genetic perturbations, high content screening is the method of choice for a deep system biology analysis. The evolution of high-content screening is mainly due to the recent progress in the development of fast and high quality automated imagers and of a plethora of new very bright fluorescent markers, so that almost any cellular element can be seen and imaged...
December 21, 2016: Chimia
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