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Shangshang Qin, Mengmeng Zhou, Qijing Zhang, Hengxun Tao, Yafei Ye, Huizhi Chen, Lijuan Xu, Hui Xu, Ping Wang, Xianju Feng
No abstract text is available yet for this article.
November 23, 2016: Emerging Microbes & Infections
Qinglan Guo, Baixing Ding, Thomas Jové, Nicole Stoesser, Vaughn S Cooper, Minggui Wang, Yohei Doi
The extended-spectrum β-lactamase gene blaCTX-M-2 is mainly associated with ISCR1 embedded in complex sul1-type integrons, but information on the genetic context of plasmids harboring the ISCR1-blaCTX-M-2 module remains limited. In this study, a blaCTX-M-2-harboring plasmid (pYD786-1) belonging to the sequence type 2 (ST2)-IncHI2 plasmid type and isolated from an Escherichia coli ST410 clinical strain was sequenced and analyzed. pYD786-1 belongs to the APEC-O1-R-type IncHI2 plasmids, which are widely distributed in human, poultry, and livestock strains...
November 2016: Antimicrobial Agents and Chemotherapy
G Gerhold, M H Schulze, U Gross, W Bohne
The increasing prevalence of extended-spectrum β-lactamase (ESBL)-producing Gram-negative bacteria is a serious threat for current healthcare settings. In this study we investigated the molecular epidemiology of ESBL-producing E. coli at the University Medical Center Göttingen in Lower Saxony, Germany. All E. coli isolates with an ESBL phenotype were collected during a 6-month period in 2014. Multilocus sequence typing and CTX-M characterization were performed on 160 isolates. Of the ESBL-producing isolates 95·6% were CTX-M positive...
June 30, 2016: Epidemiology and Infection
Julia Fischer, Katja Hille, Inga Ruddat, Alexander Mellmann, Robin Köck, Lothar Kreienbrock
Methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum β-lactamase (ESBL) producing enterobacteria (ESBL-E) have emerged in livestock. This study prospectively investigates the prevalence of MRSA and ESBL-E on pig farms and in nasal and stool samples from farmers and compares molecular characteristics of these ESBL-E isolates. In 2014, samples were derived at 51 pig farms in Germany. Per farm, five dust and five fecal samples were collected; one nasal and one stool sample were retrieved from farmers...
June 1, 2016: Veterinary Microbiology
Dorina Timofte, Iuliana Elena Maciuca, Nicola J Williams, Andrew Wattret, Vanessa Schmidt
We characterized extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) in 32 Escherichia coli extended spectrum cephalosporin (ESC)-resistant clinical isolates from UK companion animals from several clinics. In addition, to investigate the possible dissemination of ESBL clinical isolates within a veterinary hospital, two ESBL-producing E. coli isolates from a dog with septic peritonitis and a cluster of environmental ESC-resistant E. coli isolates obtained from the same clinic and during the same time period, as these two particular ESBL-positive clinical isolates, were also included in the study...
October 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Linda Falgenhauer, Can Imirzalioglu, Hiren Ghosh, Konrad Gwozdzinski, Judith Schmiedel, Katrin Gentil, Rolf Bauerfeind, Peter Kämpfer, Harald Seifert, Geovana Brenner Michael, Stefan Schwarz, Yvonne Pfeifer, Guido Werner, Michael Pietsch, Uwe Roesler, Beatriz Guerra, Jennie Fischer, Hannah Sharp, Annemarie Käsbohrer, Alexander Goesmann, Katja Hille, Lothar Kreienbrock, Trinad Chakraborty
Multidrug-resistant Escherichia coli encoding CTX-M-type extended-spectrum β-lactamases (ESBLs) are isolated in increasing numbers from humans, companion animals and livestock, raising concern regarding the exchange and spread of isolates in these populations. In this study, whole-genome sequencing of CTX-M-15-producing E. coli isolates recently sampled from humans, companion animals, livestock and farm environments was performed. In total, 26 different sequence types (STs) were detected, of which ST410 was the most frequent and was the only ST present in all populations studied...
June 2016: International Journal of Antimicrobial Agents
Ketrin C Silva, Marina Moreno, Carlos Cabrera, Beny Spira, Louise Cerdeira, Nilton Lincopan, Andrea M Moreno
We report for the first time the isolation of CTX-M-15-producingEscherichia colistrains belonging to sequence type (ST) 410, ST224, and ST1284 in commercial swine in Brazil. TheblaCTX-M-15gene was located on F-::A9::B1 and C1::A9::B1 IncF-type plasmids, surrounded by a new genetic context comprising the IS26insertion sequence truncated with the ISEcp1element upstream ofblaCTX-M-15 These results reveal that commercial swine have become a new reservoir of CTX-M-15-producing bacteria in South America.
April 2016: Antimicrobial Agents and Chemotherapy
Katharina Schaufler, Torsten Semmler, Lothar H Wieler, Michael Wöhrmann, Ramani Baddam, Niyaz Ahmed, Kerstin Müller, Axel Kola, Angelika Fruth, Christa Ewers, Sebastian Guenther
Clinically relevant extended-spectrum beta-lactamase (ESBL)-producing multi-resistant Escherichia coli have been on the rise for years. Initially restricted to mostly a clinical context, recent findings prove their prevalence in extraclinical settings independent of the original occurrence of antimicrobial resistance in the environment. To get further insights into the complex ecology of potentially clinically relevant ESBL-producing E. coli, 24 isolates from wild birds in Berlin, Germany, and 40 ESBL-producing human clinical E...
January 2016: FEMS Microbiology Ecology
Michael Pietsch, Christoph Eller, Constanze Wendt, Martin Holfelder, Linda Falgenhauer, Angelika Fruth, Tobias Grössl, Rasmus Leistner, Giuseppe Valenza, Guido Werner, Yvonne Pfeifer
The increase of Escherichia coli producing extended-spectrum β-lactamases (ESBL) in hospitals and their emergence as intestinal colonisers of healthy humans is of concern. Transmission ways and the extent of spread of distinct E. coli clones or ESBL genes among humans and animals via the food chain or the environment is a matter of debate. In this study we determined ESBL genotypes in E. coli isolates (n=233) resistant to 3rd generation cephalosporins from hospitals and medical practices using PCR and sequencing...
November 24, 2015: Veterinary Microbiology
Liang Huang, Xiaohui Wang, Yu Feng, Yi Xie, Liping Xie, Zhiyong Zong
BACKGROUND: Carbapenem resistance among the Enterobacteriaceae is a serious healthcare challenge. bla IMI is a carbapenemase gene mediating resistance to carbapenems but has not been commonly found. A bla IMI-carrying Enterobacter cloacae, which was also resistant to colistin, is reported here. FINDINGS: E. cloacae strain WCHECl-1060 was recovered from a blood sample of a leukemia patient, who was not previously exposed to colistin. Strain WCHECl-1060 belongs to a new sequence type, ST410, and was resistant to carbapenems and colistin but was susceptible to third-generation cephalosporins...
2015: Annals of Clinical Microbiology and Antimicrobials
Codruţa-Romaniţa Usein, Raluca Papagheorghe, Mihaela Oprea, Maria Condei, Monica Strãuţ
The increasing prevalence of invasive infections caused by antibiotic resistant Escherichia coli strains in Romanian patients, already mentioned in the European reports, requires better knowledge of their specific traits. Thus, a set of 38 E. coli blood isolates, collected between 2010 and 2012 at one of the local hospitals participating into the European Antimicrobial Resistance Surveillance Network, was investigated retrospectively with respect to the phylogenetic origin, extraintestinal virulence-associated markers (i...
May 2016: Folia Microbiologica
Maria Giufré, Marisa Accogli, Caterina Graziani, Luca Busani, Marina Cerquetti
Extraintestinal pathogenic Escherichia coli (ExPEC) is involved in a wide spectrum of human diseases. Chickens have been suggested as reservoirs for fluoroquinolone (FQ)-resistant ExPEC strains. Here, we report the whole-genome sequences of 4 E. coli strains sharing the same sequence type (ST) (ST410) and that were isolated from human and avian sources in Italy.
2015: Genome Announcements
Guofeng Xu, Yue Jiang, Wei An, Hongdong Wang, Xiuying Zhang
Three KPC-2-producing Escherichia coli (E1, E2, and E3) were recovered from water samples of an urban river in the city of Harbin, China. Antimicrobial susceptibility was determined by broth microdilution. Molecular characterization and genetic relatedness of the isolates were determined by polymerase chain reaction (PCR), pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and PCR-directed phylotyping. Plasmids were analyzed by conjugation, S1-PFGE, Southern blotting and PCR-based replicon typing (PBRT)...
September 2015: World Journal of Microbiology & Biotechnology
Yanbin Liu, Yu Feng, Wenjing Wu, Yi Xie, Xiaohui Wang, Xiaoxia Zhang, Xinchuan Chen, Zhiyong Zong
We report the first OXA-181-producing strain in China. blaOXA-181 was found in sequence type 410 (ST410) Escherichia coli strain WCHEC14828 from a Chinese patient without recent travel history. Genome sequencing and conjugation experiments were performed. blaOXA-181 was carried on a 51-kb self-transmissible IncX3 plasmid and was linked with qnrS1, a quinolone resistance gene. blaOXA-181 was introduced onto the IncX3 plasmid from a ColE2-type plasmid, and IncX3 plasmids have the potential to mediate the dissemination of blaOXA-181...
August 2015: Antimicrobial Agents and Chemotherapy
Thorsten Schauss, Stefanie P Glaeser, Alexandra Gütschow, Wolfgang Dott, Peter Kämpfer
The presence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli was investigated in input (manure from livestock husbandry) and output samples of six German biogas plants in 2012 (one sampling per biogas plant) and two German biogas plants investigated in an annual cycle four times in 2013/2014. ESBL-producing Escherichia coli were cultured by direct plating on CHROMagar ESBL from input samples in the range of 100 to 104 colony forming units (CFU) per g dry weight but not from output sample...
2015: PloS One
R C Rocha-Gracia, G Cortés-Cortés, P Lozano-Zarain, F Bello, Y Martínez-Laguna, C Torres
The presence of extended spectrum β-lactamase (ESBL) and plasmid-mediated AmpC β-lactamase (pAmpC) producing Escherichia coli, along with the mechanisms of antimicrobial resistance and the molecular types of isolates, was investigated in faecal samples from 53 healthy dogs in Mexico. Samples were inoculated on Levine agar plates with 2 µg/mL cefotaxime for recovery of cefotaxime-resistant (CTX(R)) E. coli. CTX(R)E. coli isolates were recovered from 9/53 (17%) samples; one isolate was characterised from each positive sample...
March 2015: Veterinary Journal
Yaochi Su, Chang-You Yu, Yilin Tsai, Shao-Hung Wang, Chihan Lee, Chishih Chu
BACKGROUND/PURPOSE: Escherichia coli is a common pathogen to cause clinical and subclinical mastitis in cows. A total of 57 E. coli isolates from raw milk from cows were characterized genetically and biochemically. METHODS: Extended-spectrum β-lactamase (ESBL) genes, the mechanism for fluoroquinolone resistance, and variations in virulence genes and genomes of these E. coli isolates were investigated by the antimicrobial susceptibility test, simplex and multiplex polymerase chain reaction (PCR), and pulsed-field gel electrophoresis (PFGE)...
December 2016: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
María-Carmen Turrientes, José-María González-Alba, Rosa del Campo, María-Rosario Baquero, Rafael Cantón, Fernando Baquero, Juan Carlos Galán
The characterization of population structures plays a main role for understanding outbreaks and the dynamics of bacterial spreading. In Escherichia coli, the widely used combination of multiplex-PCR scheme together with goeBURST has some limitations. The purpose of this study is to show that the combination of different phylogenetic approaches based on concatenated sequences of MLST genes results in a more precise assignment of E. coli phylogenetic groups, complete understanding of population structure and reconstruction of ancestral clones...
2014: PloS One
Jennie Fischer, Irene Rodríguez, Beatrice Baumann, Elisabeth Guiral, Lothar Beutin, Andreas Schroeter, Annemarie Kaesbohrer, Yvonne Pfeifer, Reiner Helmuth, Beatriz Guerra
OBJECTIVES: The characterization of CTX-M-₁₅ β-lactamase-producing Escherichia coli and Salmonella isolates originating mainly from German livestock and food. METHODS: E. coli (526, mainly commensals) and Salmonella (151) non-human isolates resistant to third-generation cephalosporins, originating from routine and monitoring submissions (2003-12) to the Federal Institute for Risk Assessment and different national targeted studies (2011-12), were examined for the presence of blaCTX-M-₁₅ genes by PCR amplification/sequencing...
November 2014: Journal of Antimicrobial Chemotherapy
J Vervoort, M Gazin, M Kazma, T Kotlovsky, C Lammens, Y Carmeli, H Goossens, S Malhotra-Kumar
The purposes of this study were to investigate the intestinal carriage of extended-spectrum β-lactamase-harbouring Enterobacteriaceae (ESBL-EN) and associated fluoroquinolone resistance (FQ-R) in 120 hospitalised patients with antibiotic-associated diarrhoea, and to investigate a correlation between Clostridium difficile (C. difficile) infection and intestinal colonisation with ESBL-EN in these patients. Stool samples were screened for C. difficile infection by toxin A/B enzyme-linked immunosorbent assay (ELISA) and for the presence of enterobacterial isolates producing β-lactamases by plating on β-lactamase screening (BLSE) agar...
December 2014: European Journal of Clinical Microbiology & Infectious Diseases
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