Agnieszka Malcher, Zuzanna Graczyk, Hermann Bauer, Tomasz Stokowy, Andrea Berman, Mikołaj Smolibowski, Dominika Blaszczyk, Piotr Jedrzejczak, Alexander N Yatsenko, Maciej Kurpisz
Infertility is a problem that affects approximately 15% of couples, and male infertility is responsible for 40-50% of these cases. The cause of male infertility is still poorly diagnosed and treated. One of the prominent causes of male infertility is disturbed spermatogenesis, which can lead to nonobstructive azoospermia (NOA). Whole-genome sequencing (WGS) allows us to identify novel rare variants in potentially NOA-associated genes, among others, in the ESX1 gene. The aim of this study was to activate the ESX1 gene using CRISPRa technology in human germ cells (testicular seminoma cells-TCam-2)...
October 2, 2023: Scientific Reports