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https://www.readbyqxmd.com/read/29142455/large-volume-paracentesis-up-to-27-l-with-adjuvant-vaginal-cabergoline-in-the-case-of-severe-ovarian-hyperstimulation-syndrome-with-successful-pregnancy-outcome-a-case-report
#1
Nupur Agarwal, Sanghamitra Ghosh, Shikha Bathwal, Baidyanath Chakravarty
Severe ovarian hyperstimulation syndrome (OHSS) is a potentially life-threatening complication of assisted reproductive technology. Herein, we report the case of an infertile couple, with the husband being azoospermic, who underwent in-vitro fertilisation and intracytoplasmic sperm injection at our institute. The woman presented with late OHSS 7 days after embryo transfer. Inpatient management was performed with intensive surveillance. Oral cabergoline was started prophylactically but was replaced by the vaginal route due to intolerance...
July 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29134181/pregnancy-and-live-birth-after-follicle-stimulating-hormone-treatment-for-an-infertile-couple-including-a-male-affected-by-sertoli-cell-only-syndrome
#2
Gianni Paulis, Luca Paulis, Gennaro Romano, Carmen Concas, Marika Di Sarno, Renata Pagano, Antonio Di Filippo, Maria Luisa Di Petrillo
In males with nonobstructive azoospermia, one of the main histopathologic patterns of the testis is Sertoli cell-only syndrome (SCOS), in which no germ cells are present and only Sertoli cells are contained in the seminiferous tubules. There is not any formal treatment for this pathological condition. However, several studies reported the possibility to perform testicular sperm extraction in patients with SCOS, although, according to some authors, sperm retrieval is possible only in the presence of focal spermatogenesis...
2017: Research and Reports in Urology
https://www.readbyqxmd.com/read/29105403/association-between-jmjd1a-expression-and-sperm-retrieval-in-non-obstructive-azoospermic-patients
#3
Zahra Eelaminejad, Raha Favaedi, Tahereh Modarresi, Marjan Sabbaghian, Mohammad Ali Sadighi Gilani, Maryam Shahhoseini
Identification of molecular markers which can predict the outcome of sperm retrieval non-invasively in patients with non-obstructive azoospermia (NOA) are valuable in clinical andrology. Jumonji domain-containing 1a (JMJD1A) is a significant epigenetic regulator during spermatogenesis, which plays an important role in the differentiation of post-meiotic germ cells into mature spermatozoa. We therefore aimed to examine the potential association between JMJD1A expression and the outcome of sperm retrieval in patients with NOA...
January 2018: Cell Journal
https://www.readbyqxmd.com/read/29101242/busulfan-administration-produces-toxic-effects-on-epididymal-morphology-and-inhibits-the-expression-of-zo-1-and-vimentin-in-the-mouse-epididymis
#4
Fang Fang, Ke Ni, Yiting Cai, Qian Zhao, Jin Shang, Xiaoke Zhang, Shiliang Shen, Chengliang Xiong
Busulfan is an alkane sulfonate currently used as an anticancer drug and to prepare azoospermic animal models, because it selectively destroys differentiated spermatogonia in the testes. However, few studies have focused on the exact effects of busulfan treatment on the epididymis currently. This study assessed the effect of busulfan on epididymal morphology and the blood-epididymis barrier in mice. We treated mice with a single injection of busulfan and detected the effect at different time points. We showed that busulfan was toxic to the morphological structure and function of the epididymis...
November 3, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29096273/effects-of-chemical-castration-using-20-cacl2-with-0-5-dmso-in-tomcats-evaluation-of-inflammatory-reaction-by-infrared-thermography-and-effectiveness-of-treatment
#5
Cristiane Sella Paranzini, Anne Kemmer Sousa, Guilherme Schiess Cardoso, Felipe Montanheiro Perencin, Luiz Guilherme Corsi Trautwein, Ana Paula Frederico Rodrigues Loureiro Bracarense, Maria Isabel Mello Martins
This study used infrared thermography to monitor the immediate inflammatory reaction to an intratesticular injection of 20% (calcium chloride) CaCl2 with 0.5% dimethyl sulfoxide (DMSO) for chemical castration and to provide evidence of the treatment's clinical viability. Six animals received a 0.25 mL intratesticular injection of 20% CaCl2 with 0.5% DMSO in each testis. Thermographic imaging, testicular measurement, penile spine evaluation, electroejaculation, and sperm analysis were performed before the injection...
October 21, 2017: Theriogenology
https://www.readbyqxmd.com/read/29082371/frequency-of-tnfr1-36-a-g-gene-polymorphism-in-azoospermic-infertile-men-a-case-control-study
#6
Hamid Reza Ashrafzadeh, Tahere Nazari, Masoud Dehghan Tezerjani, Maryam Khademi Bami, Saeed Ghasemi-Esmailabad, Nasrin Ghasemi
BACKGROUND: Tumor necrosis factor-alpha (TNF-α) is a multifunctional cytokine that regulates different cellular activities related to spermatogenesis. Tumor necrosis factor-alpha receptor 1 (TNFR1) mediates TNF-α activity and polymorphism in TNFR1 could lead to gene dysfunction and male infertility. OBJECTIVE: The aim of this study is to determine the association of TNFR1 36 A/G polymorphism with the idiopathic azoospermia in Iranian population. MATERIALS AND METHODS: This case-control study included 108 azoospermic and 119 fertile men...
August 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29079114/distinguishing-non-obstructive-azoospermia-from-obstructive-azoospermia-in-taiwanese-patients-by-hormone-profile-and-testis-size
#7
I-Shen Huang, William J Huang, Alex T Lin
BACKGROUND: An accurate diagnosis of the etiology of azoospermia is crucial, as sperm retrieval methods differ between patients with non-obstructive azoospermia (NOA) and obstructive azoospermia (OA). The aim of this study was to determine hormone and testes size cutoff values to identify the cause of azoospermia in Taiwanese patients. METHODS: The medical records of azoospermic patients were retrospectively collected from April 2008 to July 2016, including hormone profile, physical examination findings, and testes size...
October 24, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/29062795/y-chromosome-microdeletions-in-infertile-men-with-non-obstructive-azoospermia-and-severe-oligozoospermia
#8
Shin Young Kim, Hyun Jin Kim, Bom Yi Lee, So Yeon Park, Hyo Serk Lee, Ju Tae Seo
BACKGROUND: The purpose of the study was to investigate the frequencies and types of Y chromosome microdeletions in infertile men and to analyze the relationship between the levels of reproductive hormones and Y microdeletions. METHODS: A total of 1,226 infertile men were screened for Y chromosome microdeletions using multiplex PCR assay. Karyotype analysis was performed on peripheral blood lymphocytes with standard G-banding. Serum reproductive hormone levels were measured...
July 2017: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/29059046/artificial-sperm-new-horizons-in-procreation
#9
REVIEW
Gilad Amiel, Valentin Shabataev, Raanan Tal
Azoospermia, the absence of any sperm cells from the ejaculated semen, poses a real challenge to the fertility urologist. While there are options to create happy families for azoospermic couples, such as the use of donor sperm and adoption, most couples still want to have genetically related offspring. Advances in urology, gynecology, and fertility laboratory technologies allow surgical sperm retrieval in azoospermic men and achievement of live births for many, but not all azoospermic couples. At present, there are extensive research efforts in several directions to create new fertility options by creating "artificial sperm cells...
October 16, 2017: Rambam Maimonides Medical Journal
https://www.readbyqxmd.com/read/29040537/chromosomal-abnormalities-in-1663-infertile-men-with-azoospermia-the-clinical-consequences
#10
R B Donker, V Vloeberghs, H Groen, H Tournaye, C M A van Ravenswaaij-Arts, J A Land
STUDY QUESTION: What is the prevalence of chromosomal abnormalities in azoospermic men and what are the clinical consequences in terms of increased risk for absent spermatogenesis, miscarriages and offspring with congenital malformations? SUMMARY ANSWER: The prevalence of chromosomal abnormalities in azoospermia was 14.4%, and the number of azoospermic men needed to be screened (NNS) to identify one man with a chromosomal abnormality with increased risk for absence of spermatogenesis was 72, to prevent one miscarriage 370-739 and to prevent one child with congenital malformations 4751-23 757...
October 13, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29029549/progress-and-future-prospect-of-in-vitro-spermatogenesis
#11
REVIEW
Fahar Ibtisham, Jiang Wu, Mei Xiao, Lilong An, Zachary Banker, Aamir Nawab, Yi Zhao, Guanghui Li
Infertility has become a major health issue in the world. It affects the social life of couples and of all infertility cases; approximately 40-50% is due to "male factor" infertility. Male infertility could be due to genetic factors, environment or due to gonadotoxic treatment. Developments in reproductive biotechnology have made it possible to rescue fertility and uphold biological fatherhood. In vitro production of haploid male germ cell is a powerful tool, not only for the treatment of infertility including oligozoospermic or azoospermic patient, but also for the fertility preservation in pre-pubertal boys whose gonadal function is threatened by gonadotoxic therapies...
September 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29026709/needle-aspiration-biopsy-of-seminiferous-tubules-nabst-as-a-diagnostic-method-in-korea
#12
Jay Ho Jung, Tae Ho Lee, Kyung Hwa Choi, Jae Yup Hong
To evaluate the diagnostic efficacy of needle aspiration biopsy of seminiferous tubules (NABST) and to represent the redistributed diagnostic results corresponding to testicular volumes and follicle-stimulating hormone (FSH) levels. In this retrospective study, we investigated 65 infertile men with either azoospermia or oligoasthenoteratozoospermia. Following NABST, specimens were stained with hematoxylin and eosin and classified into five histological types. With pre-procedure FSH levels and testicular volumes, we evaluated the probabilities of detecting sperms within biopsy specimens...
September 2017: Chonnam Medical Journal
https://www.readbyqxmd.com/read/28974842/evaluation-of-prognostic-factors-and-determinants-in-surgical-sperm-retrieval-procedures-in-azoospermic-patients
#13
Hajrudin Spahovic, Ümit Göktolga, Dzelaludin Junuzovic, Cihan Göktaş, Admir Rama
INTRODUCTION: Main categorisation of azospermic is in two grups: nonobstructive azoospermia (NOA) and obstructive azoospermia (OA). We had evaluation of prognostic factors and determinants in sperm retrieval procedures in azoospermic patients. METHODS: Retrospective evaluation observed 21 selected patients with NOA and OA azoospermia, after that complete history, physical examination with ultarsound volume of testis and hormonal profile. Hormonal profile included: follicle stimulating hormone (FSH), luteinizing hormone (LH), testosteron (T) and prolactin (P) serum levels...
August 2017: Medical Archives
https://www.readbyqxmd.com/read/28865540/testicular-sperm-retrieval-for-intracytoplasmic-sperm-injection-in-non-azoospermic-men-when-should-we-pull-the-trigger
#14
EDITORIAL
Peter Jeffry Stahl
No abstract text is available yet for this article.
September 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28865536/varicocelectomy-before-assisted-reproductive-technology-are-outcomes-improved
#15
REVIEW
Taylor P Kohn, Jaden R Kohn, Alexander W Pastuszak
Clinical varicoceles have been associated with impaired semen parameters and male-factor infertility. Varicocele repair can improve live birth rates for men with clinical varicocele. Varicocelectomy is often combined with assisted reproductive techniques (ART) such as intrauterine insemination (IUI), in vitro fertilization (IVF), or intracytoplasmic sperm injection (ICSI). Here we review the literature examining varicocelectomy before ART to evaluate whether improved pregnancy outcomes are realized. Although insufficient evidence exists to determine if correcting a varicocele improves IUI outcomes, a clinical benefit is observed when correcting a clinical varicocele in oligospermic and nonobstructed azoospermic men before IVF/ICSI...
September 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28853108/differences-in-poly-adp-ribose-polymerase1-parp1-and-proliferative-cell-nuclear-antigen-pcna-immunoreactivity-in-patients-who-experienced-successful-and-unsuccessful-microdissection-testicular-sperm-extraction-procedures
#16
Süleyman Akarsu, Baris Büke, Seren Gülsen Gürgen, Serkan Akdemir, Funda Gode, Merve Biçer, Mustafa Agah Tekindal, Ahmet Zeki Isik
PURPOSE: The aim of this study is to evaluate expression of deoxyribonucleic acid (DNA) synthesis and repair markers in testicular tissues of azoospermic men in whom sperm retrieval could and could not be achieved as a result of microdissection testicular sperm extraction (micro-TESE) procedure. MATERIALS AND METHODS: In this prospective cohort study, testicular tissues were retrieved from 60 Non-obstructive Azoospermia (NOA) patients who underwent micro-TESE procedure...
August 29, 2017: Urology Journal
https://www.readbyqxmd.com/read/28836400/the-expression-of-tlr2-and-tlr3-in-sertoli-cells-of-azoospermic-patients
#17
Mohammad Reza Lakpour, Morteza Koruji, Abdolhossein Shahverdi, Samaneh Aghajanpour, Majid Rajabian Naghandar, Mohammad Ali Sadighi Gilani, Marjan Sabbaghian, Reza Aflatoonian
OBJECTIVES: Toll-like receptors (TLRs) on Sertoli cells are thought to have essential roles in sperm protection. This study was conducted to investigate the expression of TLR2 and TLR3 in Sertoli cells of men with azoospermia. MATERIALS AND METHODS: In this experimental study, testicular biopsies were taken from ten azoospermic men. Following enzymatic dissociation, the samples were moved to lectin coated petri dishes. After a few passages, all cells were cultivated and Seroli cells were sorted by flow cytometry...
October 2017: Cell Journal
https://www.readbyqxmd.com/read/28835195/molecular-microdeletion-analysis-of-infertile-men-with-karyotypic-y-chromosome-abnormalities
#18
Yuan Pan, Hong-Guo Zhang, Q I Xi, Han Zhang, Rui-Xue Wang, Lei-Lei Li, Rui-Zhi Liu
Objectives To investigate azoospermic factor (AZF) microdeletions in infertile men from northeastern China with karyotypic Y chromosome abnormalities. Methods G-banding of metaphase chromosomes and karyotype analysis were performed in all infertile male patients. Genomic DNA was isolated and used to analyze classical AZF microdeletions by PCR. The regions and sequence-tagged sites of AZFa (SY86, SY84), AZFb (SY127, SY134, SY143), and AZFc (SY152, SY254, SY255, SY157) were sequenced by multiplex PCR. Results A total of 190 Y chromosome abnormality carriers were found, of whom 35 had AZF microdeletions...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28799634/effectiveness-of-highly-purified-urofollitropin-treatment-in-patients-with-idiopathic-azoospermia-before-testicular-sperm-extraction
#19
Andrea Cocci, Gianmartin Cito, Giorgio I Russo, Marco Falcone, Marco Capece, Massimiliano Timpano, Pier Andrea Della Camera, Simone Morselli, Giovanni Tasso, Girolamo Morelli, Giuseppe Morgia, Andrea Minervini, Sergio Serni, Marco Carini, Alessandro Natali, Mauro Gacci
INTRODUCTION: Recent evidences demonstrated that male factor alone is responsible for about 30% cases of infertility. Human follicle-stimulating hormone (hFSH) has been introduced to increase sperm concentration, spermatogonial population, or both natural or assisted pregnancy rates (PRs) in oligozoospermic subjects with normal concentrations of gonadotropins. METHODS: Fifty infertile men affected by idiopathic azoospermia were enrolled in this study, after undergoing medical history, physical and clinical examination, baseline semen parameters and hormonal plasma concentrations...
August 7, 2017: Urologia
https://www.readbyqxmd.com/read/28778097/progress-and-future-prospect-of-in-vitro-spermatogenesis
#20
REVIEW
Fahar Ibtisham, Jiang Wu, Mei Xiao, Lilong An, Zachary Banker, Aamir Nawab, Yi Zhao, Guanghui Li
Infertility has become a major health issue in the world. It affects the social life of couples and of all infertility cases; approximately 40-50% is due to "male factor" infertility. Male infertility could be due to genetic factors, environment or due to gonadotoxic treatment. Developments in reproductive biotechnology have made it possible to rescue fertility and uphold biological fatherhood. In vitro production of haploid male germ cell is a powerful tool, not only for the treatment of infertility including oligozoospermic or azoospermic patient, but also for the fertility preservation in pre-pubertal boys whose gonadal function is threatened by gonadotoxic therapies...
July 27, 2017: Oncotarget
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