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Stem cell use in eye surgery

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https://www.readbyqxmd.com/read/27881591/vernal-keratoconjunctivitis-culmination-of-management-using-immunosuppression-surgical-and-prosthetic-therapy-over-quarter-century
#1
Shilpa Das, Anand S Pasari, Virender S Sangwan
A 22-year-old male patient presented in 1988 with active vernal keratoconjunctivitis. He was treated with topical mast cell stabilisers and corticosteroids. Chronic inflammation despite topical treatment necessitated oral immunosuppressants. Active disease came under control with this; however, the patient gradually developed limbal stem cell deficiency. He underwent bilateral pannus resection with amniotic membrane transplantation that resulted in improved ocular surface. In 2007, patient was found to have significant bilateral posterior subcapsular cataracts and underwent bilateral cataract surgery with intraocular lens implantation with good visual outcome...
November 23, 2016: BMJ Case Reports
https://www.readbyqxmd.com/read/27800245/chemotherapy-in-retinoblastoma-current-approaches
#2
REVIEW
Özge Yanık, Kaan Gündüz, Kıvılcım Yavuz, Nurdan Taçyıldız, Emel Ünal
Retinoblastoma (RB) is the most common childhood malignant intraocular tumor. Although enucleation and external beam radiotherapy have been historically used, today the most commonly used eye-sparing approach is chemotherapy. Chemotherapy can be used in both intraocular and extraocular RB cases. Chemotherapeutic agents may be applied in different ways, including systemic, subconjunctival, intra-arterial and intravitreal routes. The main purposes of application of systemic therapy are to reduce the tumor size for local treatment (chemoreduction), or to reduce the risk of metastasis after enucleation surgery (adjuvant therapy)...
December 2015: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/27504323/simple-limbal-epithelial-transplantation-in-acid-injury-and-severe-dry-eye
#3
Sudesh Kumar Arya, Anubha Bhatti, Amit Raj, Ravi Kant Bamotra
The epithelial cells of cornea constantly undergo renewal and regeneration and the stem cells responsible for renewal resides within basal epithelium at the limbus in palisades of Vogt. Simple limbal epithelial transplantation (SLET) is a simplified technique for limbal stem cell deficiency and it combines the benefits of both conjunctival limbal autografting and cultivated limbal epithelial transplantation by being single-stage and utilizing minimal donor tissue. We will be discussing two cases of ocular surface disorder which were managed successfully by new technique simple limbal epithelial transplantation...
June 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27335025/subretinal-implantation-of-a-monolayer-of-human-embryonic-stem-cell-derived-retinal-pigment-epithelium-a-feasibility-and-safety-study-in-yucat%C3%A3-n-minipigs
#4
Michael J Koss, Paulo Falabella, Francisco R Stefanini, Marcel Pfister, Biju B Thomas, Amir H Kashani, Rodrigo Brant, Danhong Zhu, Dennis O Clegg, David R Hinton, Mark S Humayun
PURPOSE: A subretinal implant termed CPCB-RPE1 is currently being developed to surgically replace dystrophic RPE in patients with dry age-related macular degeneration (AMD) and severe vision loss. CPCB-RPE1 is composed of a terminally differentiated, polarized human embryonic stem cell-derived RPE (hESC-RPE) monolayer pre-grown on a biocompatible, mesh-supported submicron parylene C membrane. The objective of the present delivery study was to assess the feasibility and 1-month safety of CPCB-RPE1 implantation in Yucatán minipigs, whose eyes are similar to human eyes in size and gross retinal anatomy...
August 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/27233037/survival-and-functionality-of-hesc-derived-retinal-pigment-epithelium-cells-cultured-as-a-monolayer-on-polymer-substrates-transplanted-in-rcs-rats
#5
Biju B Thomas, Danhong Zhu, Li Zhang, Padmaja B Thomas, Yuntao Hu, Hossein Nazari, Francisco Stefanini, Paulo Falabella, Dennis O Clegg, David R Hinton, Mark S Humayun
PURPOSE: To determine the safety, survival, and functionality of human embryonic stem cell-derived RPE (hESC-RPE) cells seeded on a polymeric substrate (rCPCB-RPE1 implant) and implanted into the subretinal (SR) space of Royal College of Surgeons (RCS) rats. METHODS: Monolayers of hESC-RPE cells cultured on parylene membrane were transplanted into the SR space of 4-week-old RCS rats. Group 1 (n = 46) received vitronectin-coated parylene membrane without cells (rMSPM+VN), group 2 (n = 59) received rCPCB-RPE1 implants, and group 3 (n = 13) served as the control group...
May 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27224077/assessment-of-surgical-outcomes-of-limbal-transplantation-using-simple-limbal-epithelial-transplantation-technique-in-patients-with-total-unilateral-limbal-deficiency
#6
Ana Gabriela Queiroz, Martina Maria Oiticica Barbosa, Myrna Serapião Santos, Telma Pereira Barreiro, José Álvaro Pereira Gomes
This study aimed to evaluate the effectiveness of the novel simple limbal epithelial transplantation (SLET) technique, which reduces the risk of iatrogenic limbal stem cell deficiency in the donor eye. Four patients with total unilateral limbal stem cell deficiency received a limbal graft, measuring 4 mm × 2 mm, from the contralateral healthy eye in a single surgical procedure. The graft was divided into 10-20 pieces and distributed on the corneal surface. At 6-month follow-up, a completely avascular corneal epithelial surface was obtained in two patients, and there was improvement in visual acuity in one patient...
April 2016: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/27206262/applications-of-stem-cell-biology-to-oculoplastic-surgery
#7
Michael G Daniel, Albert Y Wu
PURPOSE OF REVIEW: The review examines the utility of stem cell biology in ophthalmology and oculoplastic surgery. RECENT FINDINGS: The applicability of stem cell biology varies across a range of different subfields within ophthalmology and oculoplastic surgery. Resident stem cells have been identified in the lacrimal gland, corneal limbus, orbital fat, and muscles of the eye, and can potentially be applied for in-vitro cell and organ cultures with the intent of disease modeling and transplants...
September 2016: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/27164758/-retinal-cell-therapy-using-ips-cells
#8
Masayo Takahashi
Progress in basic research, starting with the work on neural stem cells in the middle 1990's to embryonic stem (ES) cells and induced pluripotent stem (iPS) cells at present, will lead the cell therapy (regenerative medicine) of various organs, including the central nervous system to a big medical field in the future. The author's group transplanted iPS cell-derived retinal pigment epithelial (RPE) cell sheets to the eye of a patient with exudative age-related macular degeneration (AMD) in 2014 as a clinical research...
March 2016: Nippon Ganka Gakkai Zasshi
https://www.readbyqxmd.com/read/27065374/an-innovative-surgical-technique-for-subretinal-transplantation-of-human-embryonic-stem-cell-derived-retinal-pigmented-epithelium-in-yucatan-mini-pigs-preliminary-results
#9
Rodrigo A Brant Fernandes, Michael Janusz Koss, Paulo Falabella, Francisco Rosa Stefanini, Mauricio Maia, Bruno Diniz, Ramiro Ribeiro, Yuntao Hu, David Hinton, Dennis O Clegg, Gerald Chader, Mark S Humayun
BACKGROUND AND OBJECTIVE: To develop a safe and efficient surgical procedure for subretinal implantation into porcine eyes of a human embryonic stem cell-derived retinal pigmented epithelium (hESC-RPE) monolayer seeded onto a Parylene-C scaffold. This implant is referred to as CPCB-RPE1. MATERIALS AND METHODS: Ultrathin Parylene-C scaffolds were seeded with hESC-RPE and surgically implanted into the subretinal space of Yucatan mini pigs (n = 8). The surgery consisted of pars plana vitrectomy, induction of a limited retinal detachment, and peripheral retinotomy for insertion of the monolayer using a novel tissue injector, followed by silicone oil tamponade injection, laser photocoagulation around the retinotomy site, and inferior iridectomy...
April 1, 2016: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/27032020/in-situ-corneal-cross-linking-for-recurrent-corneal-melting-after-boston-type-1-keratoprosthesis
#10
Gábor Tóth, Franziska Bucher, Sebastian Siebelmann, Björn Bachmann, Manuel Hermann, Nóra Szentmáry, Zoltán Zsolt Nagy, Claus Cursiefen
PURPOSE: To present a new treatment modality for recurrent corneal melting in a patient with a Boston type I keratoprosthesis (B-KPro) including in situ corneal cross-linking (CXL) and lamellar keratoplasty (LKP) as combined treatment. METHODS: Case report. RESULTS: Our report concerns a 27-year-old man whose case history involved a severe chemical burn of his left eye. After failed penetrating keratoplasty and limbal stem cell transplantation, the patient underwent B-KPro implantation...
June 2016: Cornea
https://www.readbyqxmd.com/read/26783982/optical-coherence-tomography-imaging-of-the-palisades-of-vogt-to-assist-clinical-evaluation-and-surgical-planning-in-a-case-of-limbal-stem-cell-deficiency
#11
Ladan Espandar, Jessica F Steele, Kira L Lathrop
PURPOSE: To describe the use of volumetric optical coherence tomography (OCT) imaging to assist evaluation of a patient referred for autologous limbal stem-cell transplant. METHODS: This is a case report of a 50-year-old patient presenting with unilateral limbal stem-cell deficiency who was referred for autologous limbal stem-cell transplant. The presence of Salzmann nodules in the donor eye raised questions about the efficacy of transplantation, prompting examination of both eyes using volumetric OCT imaging to determine whether there were palisades of Vogt (POV) present...
January 16, 2016: Eye & Contact Lens
https://www.readbyqxmd.com/read/26675143/derivation-of-epithelial-like-cells-from-eyelid-fat-derived-stem-cells-in-thermosensitive-hydrogel
#12
Saeed Heidari Keshel, Maryam Rostampour, Golbahar Khosropour, Atefehsadat Bandbon B, Alireza Baradaran-Rafii, Esmaeil Biazar
Injectable hydrogel is one of the great interests for tissue engineering and cell encapsulation. In the study, the thermosensitive chitosan/gelatin/β-glycerol phosphate (C/G/GP) disodium salt hydrogels were designed and investigated by different analyses. The eye fat-derived stem cells were used to evaluate the biocompatibility of hydrogels based on their phenotypic profile, viability, proliferation, and attachment ability. The results show that the sol/gel transition temperature of the C/G/GP hydrogel was in the range of 31...
2016: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/26555593/infectious-keratitis-in-limbal-stem-cell-deficiency-stevens-johnson-syndrome-versus-chemical-burn
#13
Byeong Soo Kang, Mee Kum Kim, Won Ryang Wee, Joo Youn Oh
PURPOSE: To investigate the incidence, clinical and microbiological characteristics, risk factors, and therapeutic outcome of infectious keratitis in patients with limbal stem cell deficiency (LSCD) related to Stevens-Johnson syndrome (SJS) and corneal chemical burn. METHODS: Medical records of 90 eyes of 59 patients who were diagnosed with LSCD resulting from SJS (52 eyes of 29 patients) or corneal chemical burn (38 eyes of 30 patients) were reviewed. RESULTS: Infectious keratitis developed in 35% of LSCD patients with SJS (18 eyes, 14 patients) and in 18% of those with chemical burn (7 eyes, 7 patients)...
January 2016: Cornea
https://www.readbyqxmd.com/read/26299533/limbal-stem-cell-transplantation-for-soft-contact-lens-wear-related-limbal-stem-cell-deficiency
#14
Carl Shen, Clara C Chan, Edward J Holland
PURPOSE: To describe the outcomes of limbal stem cell transplantation in eyes with limbal stem cell deficiency related to soft contact lens wear. DESIGN: Retrospective interventional case series. METHODS: Institutional database search at the Cincinnati Eye Institute revealed 9 patients (14 eyes) who underwent limbal stem cell transplantation with systemic immunosuppression for soft contact lens wear-related limbal stem cell deficiency. Outcome measures included patient demographics, symptoms, best-corrected visual acuity, ocular surface stability, adverse events, and additional surgeries required...
December 2015: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/26068024/minimally-invasive-technique-for-injection-into-rat-optic-nerve
#15
Kateryna Raykova, Melina V Jones, Hwa Huang, Paul F Hoffman, Michael Levy
The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central nervous system in a minimally-invasive surgery without bone removal. This technique describes an approach to visualization and direct injection of the optic nerve following minor fascial dissection from the orbital ridge, using a conjunctival traction suture to gently pull the eye down and out. Representative examples of an injected optic nerve show successful injection of dyed beads...
2015: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/25945538/impression-cytology-in-the-evaluation-of-ocular-surface-tumors-review-article
#16
REVIEW
Jeison de Nadai Barros, Simone Ribeiro Araújo de Almeida, Marcia Serva Lowen, Marcelo Carvalho da Cunha, José Álvaro Pereira Gomes
Impression cytology (IC) has been widely used as a method for evaluating the ocular surface and superficial cells layers in the diagnosis and follow-up after treatment of several ocular surface tumors of both epithelial and melanocytic origin. Information regarding this can be found in the English-language literature since 1992. Using either cellulose acetate or Biopore membranes for specimen collection, a high correlation has been found between IC and tissue histology. Compared with exfoliative cytology with spatula, IC is less traumatic to the patient's eye, provides a precise location of the area being studied, and allows accurate observation of the cells the way they exist in vivo...
March 2015: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/25678766/ocular-surface-disease-in-posttrabeculectomy-mitomycin-c-patients
#17
Janice Lam, Tina T Wong, Louis Tong
BACKGROUND: Our aim was to describe the demographics, risk factors, clinical signs, severity, and outcome of ocular surface disease (OSD) in 12 patients who had undergone trabeculectomy augmented with mitomycin C (MMC). METHODS: Twelve glaucoma patients were referred to the Dry Eye Clinic (Singapore National Eye Centre) for further management of clinically significant OSD. RESULTS: Of the 15 eyes from 12 patients, 14 were treated with MMC and one with 5-fluorouracil...
2015: Clinical Ophthalmology
https://www.readbyqxmd.com/read/25603439/autologous-serum-eye-drops-for-the-treatment-of-ocular-surface-disease
#18
REVIEW
Amir A Azari, Christopher J Rapuano
OBJECTIVE: To examine the evidence for the role of autologous serum eye drops (ASD) in disease of the ocular surface. METHODS: A search of the literature published through May 2014 using PubMed, the ISI Web of Knowledge database, and the Cochrane Library was performed. Qualified articles were selected after review of titles, abstracts, and references. RESULTS: There was a significant improvement in either symptoms or some of the clinical examination findings, including tear breakup time, corneal staining, Schirmer values, and impression cytology in eyes with persistent corneal epithelial defect, graft-versus-host disease, Sjögren- and non-Sjögren-related dry eye disease, limbal stem-cell deficiency, recurrent corneal erosion, superior limbic keratoconjunctivitis, and postrefractive surgery...
May 2015: Eye & Contact Lens
https://www.readbyqxmd.com/read/25568842/use-of-umbilical-cord-serum-in-ophthalmology
#19
REVIEW
Kyung Chul Yoon
Among blood preparations, serum has been topically used in the management of various ocular diseases in ophthalmology. Like peripheral blood serum, umbilical cord blood serum contains a high concentration of essential tear components, growth factors, neurotrophic factors, vitamin A, fibronectin, prealbumin, and oil. Umbilical cord serum can provide basic nutrients for epithelial renewal and can facilitate the proliferation, migration, and differentiation of the ocular surface epithelium. Eye drops made from umbilical cord serum have been applied to treat various ocular surface diseases, including severe dry eye with or without Sjögren's syndrome, ocular complications in graft-versus-host disease, persistent epithelial defects, neurotrophic keratopathy, recurrent corneal erosions, ocular chemical burn, and surface problems after corneal refractive surgery...
December 2014: Chonnam Medical Journal
https://www.readbyqxmd.com/read/25558620/-stem-cell-therapy-of-the-eye
#20
REVIEW
Heli Skottman, E Hannu Uusitalo
The structure of the eye and the currently available methods of ophthalmic surgery enable the development work on stem cell transplantations and their clinical implementation. For example the frequency of occurrence of age-related macular degeneration will increase significantly with the ageing population. Stem cell transplantations are therefore expected to be of considerable significance in the future treatment of ocular diseases. Clearly the farthest advance has been achieved with treatments developed for diseases of the cornea and the retina...
2014: Duodecim; Lääketieteellinen Aikakauskirja
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