Kathryn C Davidson, Minkyung Sung, Karl D Brown, Julian Contet, Serena Belluschi, Regan Hamel, Aida Moreno-Moral, Rodrigo L Dos Santos, Julian Gough, Jose M Polo, Mark Daniell, Geraint J Parfitt
The corneal epithelium acts as a barrier to pathogens entering the eye; corneal epithelial cells are continuously renewed by uni-potent, quiescent limbal stem cells (LSCs) located at the limbus, where the cornea transitions to conjunctiva. There has yet to be a consensus on LSC markers and their transcriptome profile is not fully understood, which may be due to using cadaveric tissue without an intact stem cell niche for transcriptomics. In this study, we addressed this problem by using single nuclei RNA sequencing (snRNAseq) on healthy human limbal tissue that was immediately snap-frozen after excision from patients undergoing cataract surgery...
March 21, 2024: Scientific Reports