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Artem Pastukhov, Natalia Krisanova, Vitalii Maksymenko, Tatiana Borisova
BACKGROUND: Both deep and profound hypothermia are effectively applied in cardiac surgery of the aortic arch, when the reduction of cerebral circulation facilitates operations, and for the prevention of ischemic stroke consequences. Neurochemical discrimination of the effects of deep and profound hypothermia (27 and 17 °C, respectively) on non-pathological and pathological ischemia-related mechanisms of presynaptic glutamate transport with its potential contribution to predictive, preventive and personalized medicine (PPPM) was performed...
2016: EPMA Journal
Sylwia Szotek, Joanna Dawidowicz, Brian Eyden, Natalia Matysiak, Aleksander Czogalla, Grzegorz Dudzik, Anna Leśniewicz, Krzysztof Maksymowicz
Fascia lata is an important element of the fascial system, which forms the continuum of connective tissue throughout the body. This deep fascia envelops the entire thigh and hip area and its main function is to transmit mechanical forces generated by the musculoskeletal system of the lower extremities. Fascia lata is also known as a useful and easily harvested graft material. Despite its crucial role in lower extremity biomechanics and wide-ranging applications in plastic and reconstructive surgery, both the structure of fascia lata and particularly the cells populating this tissue are relatively unexplored and therefore poorly understood...
November 2016: Ultrastructural Pathology
Mathieu Lemaitre, Paul Monsarrat, Vincent Blasco-Baque, Pascale Loubières, Rémy Burcelin, Louis Casteilla, Valérie Planat-Bénard, Philippe Kémoun
: : Current treatment of periodontitis is still associated with a high degree of variability in clinical outcomes. Recent advances in regenerative medicine by mesenchymal cells, including adipose stromal cells (ASC) have paved the way to improved periodontal regeneration (PD) but little is known about the biological processes involved. Here, we aimed to use syngeneic ASCs for periodontal regeneration in a new, relevant, bacteria-induced periodontitis model in mice. Periodontal defects were induced in female C57BL6/J mice by oral gavage with periodontal pathogens...
September 16, 2016: Stem Cells Translational Medicine
D Figueroa, R Martínez, R Calvo, M Scheu, M Gallegos, A Vaisman, C Martínez, A González
INTRODUCTION: For successful anterior cruciate ligament (ACL) reconstruction, revascularisation and histological maturation are necessary, as their failure can cause graft rupture. PURPOSE: The purpose of this study was to describe differences in the histological maturation of early failed plasty (less than 12 months after surgery) and late failed plasty (more than 12 months after surgery) in patients with re-rupture after ACL reconstruction with hamstring tendons...
November 2016: Revista Española de Cirugía Ortopédica y Traumatología
Rafael Mamprin Stopiglia, Ubirajara Ferreira, Daniel Gustavo Faundes, Carlos Alberto Petta
INTRODUCTION: Endometriosis is a disease with causes still unclear, affecting approximately 15% of women of reproductive age, and in 1%-2% of whom it may involve the urinary tract. The bladder is the organ most frequently affected by endometriosis, observed around 85% of the cases. In such cases, the most effective treatment is partial cystectomy, especially via videolaparoscopy. Study Objective, Design, Size and Duration: In order to identify and delimit the extent of the intravesical endometriosis lesion, to determine the resection limits, as well as to perform an optimal reconstruction of the organ aiming for its maximum preservation, we performed a cystoscopy simultaneously with the surgery, employing a modified light-tolight technique in 25 consecutive patients, from September 2006 to May 2012...
September 1, 2016: International Braz J Urol: Official Journal of the Brazilian Society of Urology
Christian Ineichen, Heide Baumann-Vogel, Markus Christen
During the last 25 years, more than 100,000 patients have been treated with Deep Brain Stimulation (DBS). While human clinical and animal preclinical research has shed light on the complex brain-signaling disturbances that underpin e.g., Parkinson's disease (PD), less information is available when it comes to complex psychosocial changes following DBS interventions. In this contribution, we propose to more thoroughly investigate complex personality-related changes following deep brain stimulation through refined and reliable instruments in order to help patients and their relatives in the post-surgery phase...
September 7, 2016: Brain Sciences
Wen-Ya Qiu, Li-Bin Zheng, Fei Pan, Bei-Bei Wang, Yu-Feng Yao
BACKGROUND: Reis-Bücklers corneal dystrophy (RBCD) was consistently reported as a corneal dystrophy only affected Bowman's layer and superficial corneal stroma, and superficial keratectomy was a recommendation surgery for treatment in literatures. The study reported new histopathological and ultrastructural findings in RBCD caused by the Arg124Leu mutation of transforming growth factor induced (TGFBI) gene in a four-generation Chinese pedigree. METHODS: Subjects including eight patients and seven unaffected family members received slit-lamp biomicroscopy and photography...
September 2, 2016: BMC Ophthalmology
Dapeng Hou, Beibei Liu, Juan Zhang, Qiushi Wang, Wei Zheng
BACKGROUND While mild and moderate sedation have been widely used to reduce sudden agitation in intracerebral hemorrhage (ICH) patients after surgery, agitation is still a frequent problem, which may cause postoperative blood pressure fluctuation. The present study aimed to evaluate the efficacy and safety of short-course deep sedation for the treatment of ICH after surgery. MATERIAL AND METHODS A total of 41 ICH patients who received surgery, including traditional craniotomy hematoma removal and decompressive craniectomy, were including in this non-randomized control study...
July 28, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Sarwat Ahmad, Keli Turner, Paulesh Shah, Jose Diaz
Mucosal discoloration of an intestinal stoma may indicate self-limited venous congestion or necrosis necessitating operative revision. A common bedside technique to assess stoma viability is the "test tube test". A clear tube is inserted into the stoma and a hand-held light is used to assess the color of the stoma. A technique (video-assisted test tube test [VATTT]) developed by the authors utilizes a standard video bronchoscope inserted into a clear plastic blood collection tube to visually inspect and assess the mucosa...
July 2016: Ostomy/wound Management
Virgínia Dias Uzêda-E-Silva, Tania Tavares Rodriguez, Isadora Almeida Rios Rocha, Flávia Calo Aquino Xavier, Jean Nunes Dos Santos, Patrícia Ramos Cury, Luciana Maria Pedreira Ramalho
The aim of this study was to evaluate the influence of laser photobiomodulation in cutaneous healing of rats under a hyperlipidic diet. Forty-eight Wistar Albinus rats, weaned, received standard diet (SD) or hyperlipidic diet (HD) for 20 weeks. The groups were divided into SD rats and HD rats, SD-irradiated rats (LSD), and HD-irradiated rats (LHD). Standard cutaneous wound (1 cm(2)) was created on the dorsum of each rat. The irradiation started immediately after surgery and every 48 h for 7 or 14 days (λ660 nm, 40 mW, 6 J/cm(2), ϕ 0,04 cm(2), CW), when they were killed under deep anesthesia...
September 2016: Lasers in Medical Science
Georgia Ramantani, Louis Maillard, Laurent Koessler
Ever since the implementation of invasive EEG recordings in the clinical setting, it has been perceived that a considerable proportion of epileptic discharges present at a cortical level are missed by routine scalp EEG recordings. Several in vitro, in vivo, and simulation studies have been performed in the past decades aiming to clarify the interrelations of cortical sources with their scalp and invasive EEG correlates. The amplitude ratio of cortical potentials to their scalp EEG correlates, the extent of the cortical area involved in the discharge, as well as the localization of the cortical source and its geometry have been each independently linked to the recording of the cortical discharge with scalp electrodes...
October 2016: Seizure: the Journal of the British Epilepsy Association
Peter Lin, Zhongnan Fang, Jia Liu, Jin Hyung Lee
The investigation of the functional connectivity of precise neural circuits across the entire intact brain can be achieved through optogenetic functional magnetic resonance imaging (ofMRI), which is a novel technique that combines the relatively high spatial resolution of high-field fMRI with the precision of optogenetic stimulation. Fiber optics that enable delivery of specific wavelengths of light deep into the brain in vivo are implanted into regions of interest in order to specifically stimulate targeted cell types that have been genetically induced to express light-sensitive trans-membrane conductance channels, called opsins...
2016: Journal of Visualized Experiments: JoVE
Yanmei Yang, Jing Mu, Bengang Xing
Among the various types of diseases, cancer remains one of the most leading causes of mortality that people are always suffering from and fighting with. So far, the effective cancer treatment demands accurate medical diagnosis, precise surgery, expensive medicine administration, which leads to a significant burden on patients, their families, and the whole national healthcare system around the world. In order to increase the therapeutic efficiency and minimize side effects in cancer treatment, various kinds of stimuli-responsive drug delivery systems and bioimaging platforms have been extensively developed within the past decades...
April 20, 2016: Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
Hyun Jin Choi, Tae-Joong Kim, Yoo-Young Lee, Jeong-Won Lee, Byoung-Gie Kim, Duk-Soo Bae
OBJECTIVE: Indocyanine green with near-infrared fluorescence imaging (NIR-ICG) is a new tracer modality in the limelight used for lymphatic mapping. The advantage of this method is to provide real-time image during surgery. To use ICG for image guided lymph node dissection, a surgeon needs to know initial appearing time and duration. METHODS: A 52-year-old woman undertook surgery diagnosed with endometrial cancer. She had no past medical history and her body mass index was 25...
May 2016: Journal of Gynecologic Oncology
Julia Tünsmeyer, Klaus Hopster, Sabine B Kästner
OBJECTIVE: To investigate the use of the Narcotrend electroencephalogram (EEG) monitor for the assessment of anesthetic depth in horses undergoing xylazine balanced isoflurane anesthesia. STUDY DESIGN: Blinded experimental study. ANIMALS: Seven healthy warm-blooded horses, aged 10.6 ± 5.9 years, weighing 535 ± 55 kg. METHODS: Horses were anesthetized for a terminal surgical trial with xylazine, thiopentone, and guaiphenesin for induction and isoflurane and xylazine continuous rate infusion for maintenance...
2016: Frontiers in Veterinary Science
Si-Wei You, Mats Hellström, Margaret A Pollett, Chrisna LeVaillant, Colette Moses, Paul J Rigby, Marissa Penrose, Jennifer Rodger, Alan R Harvey
Peripheral nerve (PN) grafts can be used to bridge tissue defects in the CNS. Using a PN-to-optic nerve (ON) graft model, we combined gene therapy with pharmacotherapy to promote the long-distance regeneration of injured adult retinal ganglion cells (RGCs). Autologous sciatic nerve was sutured onto the transected ON and the distal end immediately inserted into contralateral superior colliculus (SC). Control rats received intraocular injections of saline or adeno-associated virus (AAV) encoding GFP. In experimental groups, three bi-cistronic AAV vectors encoding ciliary neurotrophic factor (CNTF) were injected into different regions of the grafted eye...
May 2016: Experimental Neurology
Li-Hua Zou, Jin-Ping Liu, Hao Zhang, Shu-Bin Wu, Bing-Yang Ji
BACKGROUND: Antegrade selective cerebral perfusion (ASCP) is regarded to perform cerebral protection during the thoracic aorta surgery as an adjunctive technique to deep hypothermic circulatory arrest (DHCA). However, brain metabolism profile after ASCP has not been systematically investigated by metabolomics technology. METHODS: To clarify the metabolomics profiling of ASCP, 12 New Zealand white rabbits were randomly assigned into 60 min DHCA with (DHCA+ASCP [DA] group, n = 6) and without ( DHCA [D] group, n = 6) ASCP according to the random number table...
March 20, 2016: Chinese Medical Journal
Yur-Ren Kuo, Chien-Chang Chen, Yen-Chou Chen, Ming-Chung Yeh, Pao-Yuan Lin, Chen-Hsiang Lee, Jui-Kun Chang, Yi-Chun Lin, Shun-Chen Huang, Yuan-Cheng Chiang, Nien-Mu Chiu, Yu Lee, Yu-Chi Huang, Jiun-Lung Liang, Re-Wen Wu, Ka-Kit Siu, Kuan-Chih Chung, Min-Hsien Chiang, Cheng-Chung Pan, Fu-Chan Wei
PURPOSE: Hand transplantations have been initiated and have been encouraged by promising results for more than 1 decade. The aim of this study was to present the first case of hand transplantation performed in Taiwan. MATERIALS AND METHODS: On September 3, 2014, we transplanted the left distal forearm and hand of a brain-dead managed 37 years to a man aged 45 years who had traumatic amputation of the distal third of his right forearm 30 years ago. The total ischemic time during the transplantation was 6 hours and 45 minutes...
February 2016: Annals of Plastic Surgery
Alfonso Iovieno, Sonia N Yeung, Yoav Nahum, Joshua Teichman, Eugenio Lipari, Massimo Busin, Luigi Fontana
PURPOSE: To analyze corneal stromal lamellae orientation in normal subjects and patients with various corneal conditions using a polarimetric interferometer. METHODS: Thirty-two healthy control subjects (59 eyes) and 16 patients (22 eyes) with corneal conditions or postkeratoplasty were included in the study. All patients were imaged using the interferometer and slit lamp photography. The interferometer produces an orthogonal cross-like image of stromal lamellae by illuminating the cornea in phase-light polarization angle...
April 2016: Cornea
Xing Liu, Jing Wang, Baoguo Wang, Ying Hua Wang, Qinglei Teng, Jiaqing Yan, Shuangyan Wang, You Wan
BACKGROUND: Transcutaneous acupoint electrical stimulation (TAES) as a needleless acupuncture has the same effect like traditional manual acupuncture. The combination of TAES and anesthesia has been proved valid in enhancing the anesthetic effects but its mechanisms are still not clear. METHODS: In this study, we investigated the effect of TAES on anesthesia with an electroencephalogram (EEG) oscillation analysis on surgery patients anesthetized with propofol, a widely-used anesthetic in clinical practice...
2016: BMC Complementary and Alternative Medicine
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