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https://www.readbyqxmd.com/read/29679546/lithium-chloride-licl-induced-autophagy-and-downregulated-expression-of-transforming-growth-factor-%C3%AE-induced-protein-tgfbi-in-granular-corneal-dystrophy
#1
Danyao Nie, Yun Peng, Ming Li, Xinhua Liu, Meilan Zhu, Lin Ye
This study evaluated whether lithium chloride (LiCl) prevents cytoplasmic accumulation of mutant-transforming growth factor β-induced protein (Mut-TGFBI) in granular corneal dystrophy (GCD) via activation of the autophagy pathway. Levels of TGFBI and microtubule-associated protein 1A/1B-light chain 3 (LC3) in 3 GCD patients and healthy controls were analyzed by immunohistochemistry (IHC) staining and Western blot. Primary corneal fibroblasts were isolated and transfected with wild type or mutant type TGFBI over-expressed vectors, and then treated with LiCl and/or autophagy inhibitor 3-methyladenine (3-MA)...
April 18, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29679460/limbal-niche-cells-are-a-potent-resource-of-adult-mesenchymal-progenitors
#2
REVIEW
Ping Guo, Hong Sun, Yuan Zhang, Sean Tighe, Shuangling Chen, Chen-Wei Su, Yongsong Liu, Hongxia Zhao, Min Hu, Yingting Zhu
Limbal niche cells located in the limbal Palisades of Vogt are mesenchymal stem cells that reside next to limbal basal epithelial cells. Limbal niche cells are progenitors that express embryonic stem cell markers such as Nanog, Nestin, Oct4, Rex1, Sox2 and SSEA4, mesenchymal cell markers such as CD73, CD90 and CD105, and angiogenesis markers such as Flk-1, CD31, CD34, VWF, PDGFRβ and α-SMA, but negative for CD45. In addition, the stemness of limbal niche cells can be maintained during their cell culture in a three-dimension environment...
April 20, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29679087/optical-and-tectonic-corneal-transplant-outcomes-in-a-tertiary-hospital-in-singapore-within-the-singapore-corneal-transplant-registry
#3
Dawn Ho, Charmaine Chai, Hazel Lin, Hla Myint Htoon, Anna Marie Tan, Ray Manotosh, Donald Tan
INTRODUCTION: This study aimed to describe and compare corneal graft survival and optical outcomes following deep anterior lamellar keratoplasty (DALK) and Descemet's stripping automated endothelial keratoplasty (DSAEK) with penetrating keratoplasty (PK), and to document tectonic success of patch grafts. MATERIALS AND METHODS: This was a retrospective, non-randomised, comparative and descriptive cohort study. A total of 139 eyes that underwent primary keratoplasty between 2000 and 2016 were included, and the following data was extracted: demographics, clinical diagnosis and primary indication, pre- and intraoperative risk factors, postkeratoplasty outcomes, and complications...
March 2018: Annals of the Academy of Medicine, Singapore
https://www.readbyqxmd.com/read/29677363/the-effect-of-denatured-lysozyme-on-human-corneal-epithelial-cells
#4
David J McCanna, Sarah Oh, Junghee Seo, Chantal Coles-Brennan, Zohra Fadli, Lakshman N Subbaraman, Lyndon W Jones
Purpose: During contact lens wear, the amount of lysozyme deposited on contact lenses varies depending on the lens material. The binding of lysozyme to some contact lens materials may result in a conformational change that denatures the protein to an inactive form. This investigation evaluated the effect that denatured lysozyme has on human corneal epithelial cells (HCECs) by measuring cell viability and the release of inflammatory cytokines. Methods: HCECs were exposed to lysozyme that was denatured to various activity levels...
April 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29677361/alterations-of-murine-subbasal-corneal-nerves-after-environmental-dry-eye-stress
#5
Cem Simsek, Takashi Kojima, Murat Dogru, Kazuo Tsubota
Purpose: To investigate the morphologic changes in the corneal subbasal nerve (CSN) plexus in wild-type mice after exposure to environmental dry eye stress (EDES) using in vivo confocal microscopy (IVCM). Methods: We examined 22 eyes of 8-week-old wild-type male mice (Balb/c, n = 11). The mice were exposed to an air fan inside a small compartment 5 hours/day for 3 days (EDES). Aqueous tear secretion and corneal epithelial damage were assessed. The CSNs were investigated by laser-scanning IVCM...
April 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29677354/retinoic-acid-maintains-function-of-neural-crest-derived-ocular-and-craniofacial-structures-in-adult-zebrafish
#6
Bahaar Chawla, William Swain, Antionette L Williams, Brenda L Bohnsack
Purpose: Retinoic acid (RA) is required for embryonic formation of the anterior segment of the eye and craniofacial structures. The present study further investigated the role of RA in maintaining the function of these neural crest-derived structures in adult zebrafish. Methods: Morphology and histology were analyzed by using live imaging, methylacrylate sections, and TUNEL assay. Functional analysis of vision and aqueous humor outflow were assayed with real-time imaging...
April 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29677349/repeat-associated-non-atg-ran-translation-in-fuchs-endothelial-corneal-dystrophy
#7
Elisabetta Soragni, Lina Petrosyan, Tommy A Rinkoski, Eric D Wieben, Keith H Baratz, Michael P Fautsch, Joel M Gottesfeld
Purpose: The strongest genetic association with Fuchs' endothelial corneal dystrophy (FECD) is the presence of an intronic (CTG·CAG)n trinucleotide repeat (TNR) expansion in the transcription factor 4 (TCF4) gene. Repeat-associated non-ATG (RAN) translation, an unconventional protein translation mechanism that does not require an initiating ATG, has been described in many TNR expansion diseases, including myotonic dystrophy type 1 (DM1). Given the similarities between DM1 and FECD, we wished to determine whether RAN translation occurs in FECD...
April 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29677348/the-relationship-of-lid-wiper-epitheliopathy-to-ocular-surface-signs-and-symptoms
#8
Wing Li, Thao N Yeh, Tiana Leung, Tiffany Yuen, Mariel Lerma, Meng C Lin
Purpose: There has been interest in determining whether lid wiper epitheliopathy (LWE) plays a key role in causing ocular discomfort. Conflicting reports have made it difficult to discern whether LWE is more prevalent in certain populations, what characteristics are associated with its severity, and what its role is in symptomology. This cross-sectional study on a large and diverse population attempts to answer these questions. Methods: Subjects were asked to complete questionnaires related to dry eye and to ocular discomfort...
April 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29677103/ocular-delivery-system-for-propranolol-hydrochloride-based-on-nanostructured-lipid-carrier
#9
Behzad Sharif Makhmal Zadeh, Hassan Niro, Fakher Rahim, Golbarg Esfahani
One drawback of traditional forms of medical ocular dosage is drug dilution by tear; moreover, drugs are rapidly drained away from pre-corneal cavity by tear flow and lacrimo-nasal drainage. Prolonging contact time with different strategies and mucoadhesive vehicles will help to continuously deliver drugs to the eyes. For this study, we prepared and evaluated the effects of a nanostructure lipid carrier (NLC) on propranolol hydrochloride as a hydrophilic drug model for rabbit corneal permeation. Propranolol hydrochloride NLC was prepared using cold homogenization...
April 20, 2018: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/29677004/new-surgical-modality-for-management-of-corneal-perforation-using-bowman-membrane
#10
Dharamveer Singh Choudhary, Nikhil Agrawal
PURPOSE: To describe a new surgical modality, "Bowman membrane tuck-in," for management of small to medium sized corneal perforation of a noninfectious etiology. METHODS: Two patients with corneal perforation of a noninfectious etiology and less than 4 mm in size underwent Bowman membrane tuck-in, a new surgical procedure. In this technique, Bowman membrane harvested from the donor cornea was tucked inside the stromal pocket of host tissue, which was prepared by 360-degree anterior stromal lamellar dissection...
April 19, 2018: Cornea
https://www.readbyqxmd.com/read/29677003/identification-of-a-novel-tcf4-isoform-in-the-human-corneal-endothelium
#11
Allen O Eghrari, Shivakumar Vasanth, Briana C Gapsis, Henry Bison, Ula Jurkunas, S Amer Riazuddin, John D Gottsch
PURPOSE: Alternative splice isoforms of TCF4, a gene implicated in Fuchs corneal dystrophy, have been identified in multiple human tissues outside of the eye. The aim of this study was to identify the transcriptional profile of TCF4 in the corneal endothelium. METHODS: We extracted RNA from the donor corneal endothelium and performed rapid amplification of cDNA ends. We tested the expression pattern of 1 newly identified isoform (7b) in a panel of cDNA derived from multiple human tissues and included cDNA from corneal endothelial (CE) and retinal pigment epithelial cell lines...
April 19, 2018: Cornea
https://www.readbyqxmd.com/read/29676322/corneal-fibroma-an-uncommon-stromal-tumor
#12
Akshay Gopinathan Nair, Hemal Kenia, Indumati Gopinathan, Siddharth V Mehta, Vinod C Mehta
A 56-year-old male patient presented with a slow-growing, elevated, smooth, white corneal mass. The mass was excised by performing an alcohol-assisted keratoepitheliectomy and sent for histopathological examination. Subepithelially, closely packed spindle cells in "feather-stitched" or storiform pattern were seen. Immunohistochemically, the cells stained negatively for CD-34 and S-100 and focal positivity was seen for vimentin. Based on the morphology and immunochemical staining, a diagnosis of corneal fibroma was made...
May 2018: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29676313/deep-anterior-lamellar-keratoplasty-or-penetrating-keratoplasty-in-lattice-corneal-dystrophy
#13
Ritu Arora
No abstract text is available yet for this article.
May 2018: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29676312/outcomes-of-keratoplasty-in-lattice-corneal-dystrophy-in-a-large-cohort-of-indian-eyes
#14
Ashik Mohamed, Sunita Chaurasia, Muralidhar Ramappa, Somasheila I Murthy, Prashant Garg
Purpose: The purpose of this study is to evaluate the outcomes of keratoplasty for lattice corneal dystrophy (LCD) performed at a tertiary eye care center. Methods: A retrospective review of medical records of those patients who were clinically diagnosed to have LCD (72 eyes of 57 patients) and underwent either penetrating keratoplasty (PK, 58 eyes of 46 patients) or deep anterior lamellar keratoplasty (DALK, 14 eyes of 13 patients) between the years 1987 and 2014 was performed...
May 2018: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29676302/keratoprosthesis-current-global-scenario-and-a-broad-indian-perspective
#15
REVIEW
Geetha Iyer, Bhaskar Srinivasan, Shweta Agarwal, Deepti Talele, Ekta Rishi, Pukhraj Rishi, Sripriya Krishnamurthy, Lingam Vijaya, Nirmala Subramanian, Shanmugasundaram Somasundaram
Keratoprosthesis (Kpro) forms the last resort for bilateral end-stage corneal blindness. The Boston Type 1 and 2 Kpros, the modified osteo-odonto Kpro and the osteo-Kpro are the more frequently and commonly performed Kpros, and this review attempts to compile the current data available on these Kpros worldwide from large single-center studies and compare the indications and outcomes with Kpros in the Indian scenario. Although the indications have significantly expanded over the years and the complications have reduced with modifications in design and postoperative regimen, these are procedures that require an exclusive setup, and a commitment toward long-term follow-up and post-Kpro care...
May 2018: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29676012/further-audiovestibular-characterization-of-dfnb77-caused-by-deleterious-variants-in-loxhd1-and-investigation-into-the-involvement-of-fuchs-corneal-dystrophy
#16
M Wesdorp, V Schreur, A J Beynon, J Oostrik, J M van de Kamp, M W Elting, M-J H van den Boogaard, I Feenstra, R J C Admiraal, H P M Kunst, C B Hoyng, H Kremer, H G Yntema, R J E Pennings, M Schraders
This study focuses on further characterization of the audiovestibular phenotype and on genotype-phenotype correlations of DFNB77, an autosomal recessive type of hearing impairment (HI). DFNB77 is associated with disease-causing variants in LOXHD1, and is genetically and phenotypically highly heterogeneous. Heterozygous deleterious missense variants in LOXHD1 have been associated with late-onset Fuchs corneal dystrophy (FCD). However, up to now screening for FCD of heterozygous carriers in DFNB77 families has not been reported...
April 19, 2018: Clinical Genetics
https://www.readbyqxmd.com/read/29675844/delivery-of-therapeutics-for-deep-seated-ocular-conditions-status-quo
#17
REVIEW
Hubert Nguyen, Shawn Eng, Thanh Ngo, Crispin R Dass
OBJECTIVES: There is a need for research into designing effective pharmaceutical systems for delivering therapeutic drugs to the posterior of the eye for glaucoma-related pathology, macular degeneration, diabetic retinopathy, macular oedema, retinitis and choroiditis. Conventionally, eye drops have been extensively utilised for topical drug delivery to the anterior segment of the eye, but are less effective for delivery of therapeutics to the back of the eye due to significant barriers hampering drug penetration into the target intraocular tissue...
April 19, 2018: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/29675563/trans-epithelial-corneal-collagen-cross-linking-with-iontophoresis-for-progressive-keratoconus
#18
Mohammed Ali Abu Ameerh, Muawyah Dawoud Al Bdour, Maha Al-Till, Muhannad Al Faouri
PURPOSE: To evaluate the efficacy of trans-epithelial corneal collagen cross-linking (CXL) with Iontophoresis among patients with progressive keratoconus. METHODS: It is a prospective interventional study, which is based on 41 eyes of 23 patients, suffering from progressive keratoconus and treated with trans-epithelial corneal cross-linking, using iontophoresis with ETDA and trometamol-enriched riboflavin 5 phosphates 0.1% hypotonic solution (Ricrolin+, Soot Italia SpA, Italy)...
April 19, 2018: International Ophthalmology
https://www.readbyqxmd.com/read/29675396/comment-on-post-photorefractive-keratectomy-corneal-ectasia
#19
David O'Brart
No abstract text is available yet for this article.
2018: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29675394/cryopreserved-limbal-lamellar-keratoplasty-for-peripheral-corneal-and-limbal-reconstruction
#20
Hua-Tao Xie, Jing Li, Yang Liu, Dong-Ling Jiang, Rui-Fen Shen, Ming-Chang Zhang
This study aimed to evaluate the outcomes and described the recovery process of cryopreserved limbal lamellar keratoplasty (CLLK) for peripheral corneal and limbal diseases. Thirteen eyes of 12 patients with a mean age of 41±23.9y were included. The average follow-up was 12.1±5.6mo. Stable ocular surface was achieved in all eyes at last follow-up. Epithelialization originated from both recipient and graft in 9 eyes. We conclude that CLLK compensates for the shortage of donor corneas and cryopreserved limbal grafts provide epithelialization sources in ocular surface reconstruction...
2018: International Journal of Ophthalmology
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