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https://www.readbyqxmd.com/read/29458167/autosomal-dominant-deficiency-of-the-interleukin-17f-in-recurrent-aphthous-stomatitis-possible-novel-mutation-in-a-new-entity
#1
Alireza Zare Bidoki, Ahmad Massoud, Shamsolmoulouk Najafi, Mahsa Mohammadzadeh, Nima Rezaei
Recurrent Aphthous Stomatitis (RAS) is a common oral inflammatory disease with unknown pathogenesis. Although the immune system alterations could be involved in predisposition of individuals to oral candidiasis, precise etiologies of RAS have not been understood yet. A recent study showed that autosomal dominant IL17F deficiency could cause chronic mucocutaneous candidiasis. Considering the inflammatory nature of interleukin (IL)-17F and RAS, this study was performed to check any disease-associated mutation in a number of patients with RAS...
February 16, 2018: Gene
https://www.readbyqxmd.com/read/29458130/impact-of-rna-polymerase-i-inhibitor-cx-5461-on-viral-kinase-dependent-and-independent-cytomegalovirus-replication
#2
Kristen N Westdorp, Scott S Terhune
Human cytomegalovirus (HCMV) infections cause congenital birth defects and disease in immunosuppressed individuals. Antiviral compounds can control infection yet their use is restricted due to concerns of toxicity and the emergence of drug resistant strains. We have evaluated the impact of an RNA Polymerase I (Pol I) inhibitor, CX-5461 on HCMV replication. CX-5461 inhibits Pol I-mediated ribosomal DNA transcription by binding G-quadruplex DNA structures and also activates cellular stress response pathways. The addition of CX-5461 at both early and late stages of the HCMV infection inhibited viral DNA synthesis and virus production...
February 16, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29457757/structural-variability-of-dna-containing-mg-pyrophosphate-microparticles-optimized-conditions-to-produce-particles-with-desired-size-and-morphology
#3
Vasily N Danilevich, Andrey L Mulyukin, Andrey V Machulin, Vladimir V Sorokin, Sergey A Kozlov
Our previous studies demonstrated the formation of structurally diverse DNA-containing microparticles (DNA MPs) in PCR with Mg-pyrophosphate (MgPPi) as the structure-forming component. These DNA MPs were referred to major structural types: microdisks (2D MPs) with nanometer thickness and 3D MPs with sophisticated morphology and constructed from intersecting disks and their segments. Little is known about factors that influence both the morphology and size of DNA MPs, and the present study was aimed at fulfilling this gap...
February 19, 2018: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29457584/immunological-stability-of-clostridium-difficile-toxins-in-clinical-specimens
#4
Donna M Schora, Lance R Peterson, Elena A Usacheva
OBJECTIVE The impact of storage on stability and detection of Clostridium difficile toxins in feces is poorly understood. The objective of this study was to investigate the immunological stability of C. difficile toxins in clinical stool specimens under different storage conditions by evaluating this stability using toxin detection by enzyme immunoassay (EIA). METHODS Stool specimens positive for C. difficile infection (CDI) by quantitative polymerase chain reaction (qPCR) were used for EIA testing with the C...
February 19, 2018: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/29456985/evaluation-of-mir-21-inhibition-and-its-impact-on-cancer-susceptibility-candidate-2-long-noncoding-rna-in-colorectal-cancer-cell-line
#5
Miganoosh Simonian, Mohammadreza Sharifi, Reza Nedaeinia, Meysam Mosallaie, Sharifeh Khosravi, Amir Avan, Majid Ghayour-Mobarhan, Hadi Bagheri, Rasoul Salehi
Background: Both microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) have been shown to have a critical role in the regulation of cellular processes such as cell growth and apoptosis, as well as cancer progression and metastasis. lncRNAs are aberrantly expressed in many diseases including cancer. Although it is well known that miRNAs can target a large number of protein-coding genes, little is known whether miRNAs can also target lncRNAs. In the present study, we determine whether miR-21 can regulate lncRNA cancer susceptibility candidate 2 (CASC2) in colorectal cancer...
2018: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29456981/prevalence-of-ctx-m-and-tem-%C3%AE-lactamases-in-klebsiella-pneumoniae-isolates-from-patients-with-urinary-tract-infection-al-zahra-hospital-isfahan-iran
#6
Nafiseh Maleki, Zahra Tahanasab, Sina Mobasherizadeh, Aliakbar Rezaei, Jamshid Faghri
Background: Extended-spectrum β-lactamase (ESBL)-producing is a significant resistant mechanism to β-lactams in Enterobacteriaceae , especially in Klebsiella pneumoniae . The main objectives of this study were to genetically characterize urinary clinical isolates of K. pneumoniae through the investigating of blaTEM , blaCTX-M and using molecular typing by Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) method. We also determined the frequency of antibiotic resistance of K...
2018: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29456976/respiratory-viruses-in-febrile-neutropenic-patients-with-respiratory-symptoms
#7
Mohsen Meidani, Seyed Alireza Mirmohammad Sadeghi
Background: Respiratory infections are a frequent cause of fever in neutropenic patients, whereas respiratory viral infections are not frequently considered as a diagnosis, which causes high morbidity and mortality in these patients. Materials and Methods: This prospective study was performed on 36 patients with neutropenia who admitted to hospital were eligible for inclusion with fever (single temperature of >38.3°C or a sustained temperature of >38°C for more than 1 h), upper and lower respiratory symptoms...
2018: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29456893/the-effect-of-supragingival-glycine-air-polishing-on-periodontitis-during-maintenance-therapy-a-randomized-controlled-trial
#8
Hongye Lu, Lu He, Yibing Zhao, Huanxin Meng
Background: Glycine air polishing has been proved to be safe, comfortable and time-saving. Whether it could substitute ultrasonic scaling to remove dental plaque biofilm during periodontal maintenance remains unclear. The purposes of this study were to evaluate the effect of supragingival glycine air polishing (SGAP) on the subgingival periodontal pathogens during maintenance therapy and to check the association of periodontal pathogens and clinical parameters. Methods: Twenty-three chronic periodontitis patients during their maintenance therapy were enrolled in the 12-week study...
2018: PeerJ
https://www.readbyqxmd.com/read/29456867/heterologous-and-endogenous-u6-snrna-promoters-enable-crispr-cas9-mediated-genome-editing-in-aspergillus-niger
#9
Xiaomei Zheng, Ping Zheng, Jibin Sun, Zhang Kun, Yanhe Ma
Background: U6 promoters have been used for single guide RNA (sgRNA) transcription in the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas9) genome editing system. However, no available U6 promoters have been identified in Aspergillus niger, which is an important industrial platform for organic acid and protein production. Two CRISPR/Cas9 systems established in A. niger have recourse to the RNA polymerase II promoter or in vitro transcription for sgRNA synthesis, but these approaches generally increase cloning efforts and genetic manipulation...
2018: Fungal Biology and Biotechnology
https://www.readbyqxmd.com/read/29456819/comparison-of-the-efficacy-of-piascledine-and-transforming-growth-factor-%C3%AE-1-on-chondrogenic-differentiation-of-human-adipose-derived-stem-cells-in-fibrin-and-fibrin-alginate-scaffolds
#10
Batool Hashemibeni, Ali Valiani, Mojtaba Esmaeli, Mohammad Kazemi, Maryam Aliakbari, Farhad Golshan Iranpour
Objectives: The aim of this study was to compare the chondrogenic induction potential of Piascledine and TGF-β1 on adipose-derived stem cells (ADSCs) in fibrin and fibrin-alginate scaffolds. Materials and Methods: Human subcutaneous adipose tissues were harvested from three patients who were scheduled to undergo liposuction. Isolated ADSCs were proliferated in a culture medium. Then, the cells were seeded in fibrin or fibrin-alginate scaffolds and cultured for 14 days in a chondrogenic medium containing Piascledine, TGF-β1, or both...
February 2018: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29456814/ascorbic-acid-augments-colony-spreading-by-reducing-biofilm-formation-of-methicillin-resistant-staphylococcus-aureus
#11
Zulfiqar Ali Mirani, Muhammad Naseem Khan, Anila Siddiqui, Fouzia Khan, Mubashir Aziz, Shagufta Naz, Ayaz Ahmed, Seema Ismat Khan
Objectives: Staphylococcus aureus is a Gram-positive pathogen, well known for its resistance and versatile lifestyle. Under unfavourable conditions, it adapts biofilm mode of growth. For staphylococcal biofilm formation, production of extracellular polymeric substances (EPS) is a pre-requisite, which is regulated by ica operon-encoded enzymes. This study was designed to know the impact of ascorbic acid on biofilm formation and colony spreading processes of S. aureus and MRSA. Materials and Methods: The isolates of methicillin-resistant S...
February 2018: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29456809/hypericin-photodynamic-therapy-inhibits-proliferation-and-induces-apoptosis-in-human-rheumatoid-arthritis-fibroblast-like-synoviocytes-cell-line-mh7a
#12
Kun Zhang, Shan Gao, JiaYi Guo, GuoHua Ni, Zhe Chen, Feng Li, XiaoLei Zhu, YongBing Wen, YanXing Guo
Objectives: To elucidate the effects and potential mechanisms of hypericin-photodynamic therapy (HYP-PDT) for treating the human rheumatoid arthritis (RA) fibroblast-like synoviocyte (FLS) MH7A cell-line. Materials and Methods: MH7A cells were subjected to HYP-PDT intervention and apoptosis was evaluated via MTT, nuclear staining, and flowcytometry analyses. Intracellular reactive oxygen species (ROS) were measured with the fluorescent probe 2'7'-dichlorofluorescein diacetate (DCFH-DA)...
February 2018: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29456742/mir302a-inhibits-the-proliferation-of-esophageal-cancer-cells-through-the-mapk-and-pi3k-akt-signaling-pathways
#13
Daokui Xia, Shumei Tian, Zhen Chen, Wenchao Qin, Quan Liu
MicroRNAs (miRNAs/miRs) are involved in the regulation of various types of cancer, either as oncogenes or tumor suppressors. miR302a has been reported that it could suppress tumor cell proliferation by inhibiting Akt in prostate cancer. The present study examined the effect of miR302a on proliferation and invasion in esophageal cancer cell lines. The expression levels of miR302a in esophageal cancer cell lines was determined by reverse transcription-polymerase chain reaction. Subsequently, miR302a mimics were transfected into esophageal cancer cells, and cell viability and invasion were assessed by MTT and Transwell assays...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29456732/two-protein-coding-genes-act-as-a-novel-clinical-signature-to-predict-prognosis-in-patients-with-ovarian-serous-cystadenocarcinoma
#14
Jue Zhang, Meng Xu, Han Gao, Jin-Chen Guo, Yu-Lin Guo, Miao Zou, Xu-Feng Wu
Ovarian cancer is the seventh most common type of cancer and the eighth most common cause of cancer-associated mortality among women. A number of studies have hypothesized that the expression status of certain genes may be used to predict prognosis in ovarian cancer. In the present study, the RNA expression data from next-generation sequencing and the clinical information of 413 patients from The Cancer Genome Atlas dataset was downloaded to identify the association between gene-expression level and the survival time of the patients with ovarian serous cystadenocarcinoma...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29456730/the-regulation-and-function-of-microrna-377-rassf8-signaling-axis-in-gastric-cancer
#15
Xiaobo Bo, Yusheng Chen, Weizhong Sheng, Yuda Gong, Haiyu Wang, Weidong Gao, Bo Zhang
Gastric cancer is a major cause of cancer-associated mortality worldwide. The aberrant expression of microRNA (miRNA) is involved in tumorigenesis. Ras proteins transfer information from the extracellular environment to internal cellular compartments and are essential in numerous signal transduction pathways. To investigate the regulation, function and clinical significance of the miRNA377/Ras association domain family (RASSF) 8 signaling axis in gastric cancer, reverse transcription-quantitative polymerase chain reaction, immunohistochemistry, cell counting kit-8, western blotting, and Transwell assays were used...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29456728/values-of-mir-34a-and-mir-218-expression-in-the-diagnosis-of-cervical-cancer-and-the-prediction-of-prognosis
#16
Ping Wang, Guanyun Zhai, Yunyan Bai
The expression levels of microribonucleic acid 34a (miR-34a) and miR-218 in the serum and tumor tissues of patients with cervical cancer were investigated to explore their relationship with the diagnosis of cervical cancer. Fifty patients with cervical cancer were selected, and 30 normal patients were selected as the control group. The expression levels of miR-34a and miR-218 were detected by quantitative real-time polymerase chain reaction. The relationship between the expression of miR-34a and miR-218 was analyzed using Pearson's correlation coefficient, and the receiver operating characteristic (ROC) curve was plotted for the analysis of values of the expression of miR-34a and miR-218...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29456724/long-noncoding-rna-blacat1-promotes-cell-proliferation-and-invasion-in-human-cervical-cancer
#17
Dan Shan, Yumin Shang, Tongxiu Hu
Cervical cancer is one of the leading causes of mortality in females worldwide. Predisposition to distant metastasis has reduced the prognosis of this malignancy, thus the identification of a novel agent for metastatic cervical cancer is required. Long noncoding RNAs (LncRNAs) have been reported to serve significant roles in human tumorigenesis. The present study aimed to investigate the effects of a newly discovered LncRNA bladder cancer associated transcript 1 (non-protein coding) (BLACAT1) on cell proliferation and metastasis in cervical cancer...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29456723/mir-96-promotes-breast-cancer-metastasis-by-suppressing-mtss1
#18
Wei Xie, Feng Sun, Lin Chen, Xinjian Cao
Novel, non-invasive biomarkers with high sensitivity and specificity are critical for breast cancer treatment, and prognosis. MicroRNA (miR)-96 has been demonstrated to be highly expressed in several solid malignancies, including breast cancer. However, its expression and function in the metastasis and prognosis of breast cancer have not been fully explored, and its regulation mechanisms remain unclear. In the present study, the serum miR-96 expression in healthy controls, benign and malignant breast cancer types was compared by using reverse transcription-quantitative polymerase chain reaction...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29456720/mir-494-inhibits-cervical-cancer-cell-proliferation-through-upregulation-of-socs6-expression
#19
Lei Cheng, Beihua Kong, Ying Zhao, Jie Jiang
It is unclear how microRNA (miR)-494 inhibits the proliferation of cervical cancer cells by altering the expression of SOCS6. Therefore, the present study aimed to investigate the molecular mechanism underlying miR-494 regulation of suppressor of cytokine signaling 6 (SOCS6) in human cervical cancer samples and the human cervical cancer HeLa cell line. The expression of miR-494 was determined using reverse transcription-quantitative polymerase chain reaction. In addition, TargetScan was used to predict miR-494 target genes and the luciferase reporter assay was used to determine whether SOCS6 was a direct target of miR-494...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29456719/estrogen-receptor-%C3%AE-promoter-methylation-is-a-biomarker-for-outcome-prediction-of-cisplatin-resistance-in-triple-negative-breast-cancer
#20
Junnan Xu, Tao Sun, Xiangyu Guo, Yan Wang, Mingxi Jing
A number of previous studies have indicated the presence of a link between estrogen receptor-α (ERα) methylation and triple-negative breast cancer (TNBC). However, the association between ERα methylation and drug resistance during the treatment of TNBC remains unclear. Methylation-specific polymerase chain reaction was used to investigate the methylation of ERα in the genomic DNA of 35 patients with TNBC who were defined as cisplatin-based chemotherapy-resistant using chemosensitivity testing. Survival probabilities by covariates were assessed using Kaplan-Meier estimator survival analysis and Cox's proportional hazards models, adjusting for age, menopausal status, tumor size, lymph node metastasis and ERα promoter DNA methylation...
March 2018: Oncology Letters
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