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https://www.readbyqxmd.com/read/28231660/-effect-of-telbivudine-on-infants-born-to-hbsag-positive-mothers-with-non-hypo-response-to-hepatitis-b-vaccine-during-their-second-and-third-trimesters-of-pregnancy
#1
X X Xu, B Wang, X F Wang, H X Wen, F Zhang, Z Q Yang, H Y Hao, T Wang, X H Shi, Z D Fu, B Wang, S P Wang
Objective: To explore the effect of telbivudine treatment in a prevention program on infants born to HBsAg-positive mothers with non-/hypo-responsiveness to hepatitis B vaccine. Methods: A retrospective cohort study with a total of 321 HBsAg-positive pregnant women and their infants enrolled, was conducted. The mothers were recruited from the Third People' s Hospital of Taiyuan, from July 2011 to January 2013. According to the situation of telbivudine intake in second and third trimesters of pregnancy, the participants were divided into two groups: with telbivudine-treated or as control...
February 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28231507/the-phytochelatin-synthase-gene-in-date-palm-phoenix-dactylifera-l-phylogeny-evolution-and-expression
#2
Chaâbene Zayneb, Rekik Hakim Imen, Kriaa Walid, C Douglas Grubb, Khemakhem Bassem, Vandenbulcke Franck, Mejdoub Hafedh, Elleuch Amine
We studied date palm phytochelatin synthase type I (PdPCS1), which catalyzes the cytosolic synthesis of phytochelatins (PCs), a heavy metal binding protein, in plant cells. The gene encoding PdPCS1 (Pdpcs) consists of 8 exons and 7 introns and encodes a protein of 528 amino acids. PCs gene history was studied using Notung phylogeny. During evolution, gene loss from several lineages was predicted including Proteobacteria, Bilateria and Brassicaceae. In addition, eleven gene duplication events appeared toward interior nodes of the reconciled tree and four gene duplication events appeared toward the external nodes...
February 20, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28231382/accurate-measurement-of-transgene-copy-number-in-crop-plants-using-droplet-digital-pcr
#3
Ray Collier, Kasturi Dasgupta, Yan-Ping Xing, Bryan Tarape Hernandez, Min Shao, Dominica Rohozinski, Emma Kovak, Jeanie Lin, Maria Luiza P de Oliveira, Ed Stover, Kent F McCue, Frank G Harmon, Ann Blechl, James G Thomson, Roger Thilmony
Genetic transformation is a powerful means for the improvement of crop plants, but requires labor and resource intensive methods. An efficient method for identifying single copy transgene insertion events from a population of independent transgenic lines is desirable. Currently transgene copy number is estimated by either Southern blot hybridization analyses or quantitative polymerase chain reaction (qPCR) experiments. Southern hybridization is a convincing and reliable method, but it also is expensive, time-consuming and often requires a large amount of genomic DNA and radioactively labeled probes...
February 23, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28231234/notes-from-the-field-francisella-tularensis-type-b-infection-from-a-fish-hook-injury-minnesota-2016
#4
Tory Whitten, Jenna Bjork, Dave Neitzel, Kirk Smith, Maureen Sullivan, Joni Scheftel
On June 27, 2016, the Minnesota Department of Health (MDH) Public Health Laboratory (PHL) was notified of a suspected Francisella tularensis isolate cultured at a hospital laboratory. The isolate was confirmed as F. tularensis type B at MDH PHL by reverse transcription-polymerase chain reaction, culture, and direct fluorescent antibody testing. Francisella tularensis subspecies tularensis (type A) and holarctica (type B) bacteria are the causative agents of tularemia.
February 24, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/28231073/gene-expression-profiles-suggest-iron-transport-pathway-in-the-lactating-human-epithelial-cell
#5
Chenxi Cai, Peter Eck, James K Friel
OBJECTIVES: The molecular background of iron excretion into breast milk has not been determined in humans. We determined the expression of known iron transporters in mRNA extracted from human milk fat globules to deduce which known transporters are responsible for iron excretion into human milk. METHODS: The expression of iron transporters in mRNA from human milk fat globules and mouse mammary epithelial cell lines was determined by quantitative real-time polymerase chain reaction...
March 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28230826/modifying-effects-of-5-azacytidine-on-metal-containing-proteins-profile-in-guerin-carcinoma-with-different-sensitivity-to-cytostatics
#6
V F Chekhun, Y V Lozovska, L A Naleskina, T V Borikun, A P Burlaka, I N Todor, D V Demash, T M Yalovenko, T V Zadvornyi, A O Pavlova, D M Storchay, N Yu Lukianova
AIM: To assess the influence of the treatment with 5-azacytidine (5-aza) on the profile of metal-containing proteins and factors of their regulation in Guerin carcinoma cells in vivo. MATERIALS AND METHODS: The study was conducted on Wistar rats transplanted with wild-type Guerin carcinoma (Guerin/WT) and its strains resistant to cisplatin (Guerin/CP) or doxorubicin (Guerin/Dox). Animals were distributed in 6 groups treated with 5-aza and control animals without treatment...
December 2016: Experimental Oncology
https://www.readbyqxmd.com/read/28230823/polymorphism-of-dna-repair-gene-xpd-lys751gln-and-chromosome-aberrations-in-lymphocytes-of-thyroid-cancer-patients-exposed-to-ionizing-radiation-due-to-the-chornobyl-accident
#7
V M Shkarupa, O Y Mishcheniuk, S O Henyk-Berezovska, V O Palamarchuk, S V Klymenko
: The aim of this work was to analyze the relationship between polymorphisms of DNA repair gene XPD Lys751Gln and frequency and spectrum of chromosome aberrations in the culture of peripheral blood lymphocytes of thyroid cancer (TC) patients having been exposed to ionizing radiation due to the Chornobyl accident. MATERIALS AND METHODS: XPD Lys751Gln polymorphisms were detected by polymerase chain reaction in 102 TC patients including 38 patients exposed to ionizing radiation due to Chornobyl disaster (Chornobyl recovery workers, evacuees, and the residents of contaminated areas), 64 patients without history of ionizing radiation exposure and 45 healthy residents of Ukraine as control group...
December 2016: Experimental Oncology
https://www.readbyqxmd.com/read/28230820/the-distribution-of-tp53-gene-polymorphisms-in-chronic-lymphocytic-leukemia-patients-sufferers-of-chornobyl-nuclear-power-plant-accident
#8
N I Bilous, I V Abramenko, A A Chumak, I S Dyagil, Z V Martina
: Previous analyses in a cohort of Chornobyl cleanup workers revealed significantly increased radiation-related risk for all leukemia types, including chronic lymphocytic leukemia (CLL). Numerous investigations emphasized the significance of genetic susceptibility to the radiation carcinogenesis. The aim of the work was to study the distribution of TP53 single nucleotide polymorphisms (SNPs) in CLL patients exposed to ionizing radiation (IR) due to Chornobyl nuclear power plant accident and estimate their impact on disease development...
December 2016: Experimental Oncology
https://www.readbyqxmd.com/read/28230817/common%C3%A2-chemical%C3%A2-inductors%C3%A2-of%C3%A2-replication%C3%A2-stress-%C3%A2-focus%C3%A2-on%C3%A2-cell-based%C3%A2-studies
#9
REVIEW
Eva Vesela, Katarina Chroma, Zsofia Turi, Martin Mistrik
DNA replication is a highly demanding process regarding the energy and material supply and must be precisely regulated, involving multiple cellular feedbacks. The slowing down or stalling of DNA synthesis and/or replication forks is referred to as replication stress (RS). Owing to the complexity and requirements of replication, a plethora of factors may interfere and challenge the genome stability, cell survival or affect the whole organism. This review outlines chemical compounds that are known inducers of RS and commonly used in laboratory research...
February 21, 2017: Biomolecules
https://www.readbyqxmd.com/read/28230813/characterization-of-carbonic-anhydrase-9-in-the-alimentary-canal-of-aedes-aegypti-and-its-relationship-to-homologous-mosquito-carbonic-anhydrases
#10
Daniel P Dixon, Leslie Van Ekeris, Paul J Linser
In the mosquito midgut, luminal pH regulation and cellular ion transport processes are important for the digestion of food and maintenance of cellular homeostasis. pH regulation in the mosquito gut is affected by the vectorial movement of the principal ions including bicarbonate/carbonate and protons. As in all metazoans, mosquitoes employ the product of aerobic metabolism carbon dioxide in its bicarbonate/carbonate form as one of the major buffers of cellular and extracellular pH. The conversion of metabolic carbon dioxide to bicarbonate/carbonate is accomplished by a family of enzymes encoded by the carbonic anhydrase gene family...
February 21, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28230286/identification-of-nontuberculous-mycobacteria-using-multilocous-sequence-analysis-of-16s-rrna-hsp65-and-rpob
#11
Si Hyun Kim, Jeong Hwan Shin
BACKGROUND: The isolation of nontuberculous mycobacteria (NTM) from clinical specimens has increased, and they now are considered significant opportunistic pathogens. The aims of this study were to develop a database and interpretive criteria for identifying individual species. In addition, using clinical isolates, we evaluated the clinical usefulness of 16S rRNA, hsp65, and rpoB as target genes for this method. METHODS: The sequences of NTM for 16S rRNA, hsp65, and rpoB were collected from GenBank and checked by manual inspection...
February 23, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28230172/evidence-for-critical-role-of-lymphocyte-cytosolic-protein-1-in-oral-cancer
#12
Nao Koide, Atsushi Kasamatsu, Yosuke Endo-Sakamoto, Sho Ishida, Toshihiro Shimizu, Yasushi Kimura, Isao Miyamoto, Shusaku Yoshimura, Masashi Shiiba, Hideki Tanzawa, Katsuhiro Uzawa
Lymphocyte cytosolic protein 1 (LCP1), a member of actin-binding protein of the plastin family, has been identified in several malignant tumors of non-hematopoietic sites, such as the colon, prostate, and breast. However, little is known about the roles of LCP1 in oral squamous cell carcinomas (OSCCs). This present study sought to clarify the clinical relevance of LCP1 in OSCCs and investigate possible clinical applications for treating OSCCs by regulating LCP1 expression. We found up-regulation of LCP1in OSCCs compared with normal counterparts using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunoblotting, and immunohistochemistry (P < 0...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28230059/synergistic-effects-of-dimethyloxallyl-glycine-and-recombinant-human-bone-morphogenetic-protein-2-on-repair-of-critical-sized-bone-defects-in-rats
#13
Xin Qi, Yang Liu, Zhen-Yu Ding, Jia-Qing Cao, Jing-Huan Huang, Jie-Yuan Zhang, Wei-Tao Jia, Jing Wang, Chang-Sheng Liu, Xiao-Lin Li
In bone remodeling, osteogenesis is closely coupled to angiogenesis. Bone tissue engineering using multifunctional bioactive materials is a promising technique which has the ability to simultaneously stimulate osteogenesis and angiogenesis for repair of bone defects. We developed mesoporous bioactive glass (MBG)-doped poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) composite scaffolds as delivery vehicle. Two bioactive molecules, dimethyloxalylglycine (DMOG), a small-molecule angiogenic drug, and recombinant human bone morphogenetic protein-2 (rhBMP-2), an osteoinductive growth factor, were co-incorporated into the scaffold...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28229992/increased-5-hydroxymethylcytosine-and-ten-eleven-translocation-protein-expression-in-ultraviolet-b-irradiated-hacat-cells
#14
Dan Wang, Jin-Hua Huang, Qing-Hai Zeng, Can Gu, Shu Ding, Jian-Yun Lu, Jing Chen, Sheng-Bo Yang
BACKGROUND: DNA hydroxymethylation refers to a chemical modification process in which 5-methylcytosine (5mC) is catalyzed to 5- hydroxymethylcytosine (5hmC) by ten-eleven translocation (TET) family proteins. Recent studies have revealed that aberrant TETs expression or 5hmC level may play important roles in the occurrence and development of various pathological and physiological processes including cancer and aging. This study aimed to explore the relation between aberrant DNA hydroxymethylation with skin photoaging and to investigate the levels of TETs, 5mC, and 5hmC expression 24 h after 40 mJ/cm2 and 80 mJ/cm2 doses of ultraviolet B (UVB) irradiation to HaCaT cells...
2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28229988/endometrial-microrna-signature-during-the-window-of-implantation-changed-in-patients-with-repeated-implantation-failure
#15
Cheng Shi, Huan Shen, Li-Juan Fan, Jing Guan, Xin-Bang Zheng, Xi Chen, Rong Liang, Xiao-Wei Zhang, Qing-Hua Cui, Kun-Kun Sun, Zhu-Ran Zhao, Hong-Jing Han
BACKGROUND: At present, a diagnostic tool with high specificity for impaired endometrial receptivity, which may lead to implantation failure, remains to be developed. We aimed to assess the different endometrial microRNA (miRNA) signatures for impaired endometrial receptivity by microarray analysis. METHODS: A total of 12 repeated implantation failure (RIF) patients and 10 infertile patients, who conceived and delivered after one embryo transfer attempt, were recruited as RIF and control groups, respectively...
2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28229921/protective-effect-of-a-polysaccharide-from-anoectochilus-roxburghii-against-carbon-tetrachloride-induced-acute-liver-injury-in-mice
#16
Biyu Zeng, Minghua Su, Qingxi Chen, Qiang Chang, Wei Wang, Huihua Li
ETHNOPHARMACOLOGICAL RELEVANCE: Anoectochilus roxburghii (Wall.) Lindl. is traditionally used for the treatment of various types of chronic and acute hepatitis in China. Considering that Anoectochilus roxburghii polysaccharide (ARPT) is the main constituent of Anoectochilus roxburghii, the present study was designed to investigate the hepatoprotective effect of ARPT and its possible mechanism in carbon tetrachloride (CCl4)-induced mice. MATERIAL AND METHODS: The hepatoprotective activity of ARPT (150, 300 and 500 mg/kg) were investigated on CCl4-induced acute liver damage in mice...
February 13, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28229900/interleukin-6-572c-g-polymorphism-is-associated-with-serum-interleukin-6-levels-and-risk-of-idiopathic-pulmonary-arterial-hypertension
#17
Ming Fang, Yueye Huang, Yuan Zhang, Zhongping Ning, Luoning Zhu, Xinming Li
Interleukin 6 (IL-6) is a multifunctional proinflammatory cytokine that is elevated in patients with pulmonary arterial hypertension (PAH). Single nucleotide polymorphisms in the promoter region of IL-6 have been reported to transcriptional regulate the expression of IL-6. The aim of the present study is to investigate the roles of two common polymorphisms (-572C/G [rs1800796] and -6331T/C [rs10499563]) of IL-6 in idiopathic PAH (IPAH). A total of 338 IPAH patients and 352 age- and gender-matched healthy controls were enrolled...
February 3, 2017: Journal of the American Society of Hypertension: JASH
https://www.readbyqxmd.com/read/28229498/bax-and-bcl-2-are-involved-in-the-apoptosis-induced-by-local-testicular-heating-in-the-boar-testis
#18
H Xi, X Fan, Z Zhang, Y Liang, Q Li, J He
The aim of this study was to determine whether the effect of Bax and Bcl-2 on the apoptosis of germ cells is caused by local testicular heating (42°C, 1 hr) in boar testis. The testes of three boars were exposed to 42°C for 1 hr. Three other boars were assigned as control (no heat treatment). After 6 hr of heat treatment, all boars were castrated and the testes were harvested. Immunohistochemical results showed that a redistribution of Bax was caused by heat stress, and Bcl-2 was expressed in the cytoplasm and nucleus...
February 23, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28229454/analysis-of-a-fully-penetrant-spinocerebellar-ataxia-type-8-brazilian-family
#19
V P Cintra, C M Lourenço, M M V Rocha, P J Tomaselli, W Marques
Spinocerebellar ataxia type 8 (SCA8) is a progressive neurological disorder caused by the expanded repeat CTA/CTG of two overlapping genes, ATXN8OS and ATXN8, expressed bidirectionally. Normal alleles have 15-50 repeats, and pathogenic alleles range from 71 to 1300 repeats. The disorder is relatively rare, accounting for about 2%-5% of the autosomal dominant forms of hereditary ataxia worldwide. However, the prevalence of disease-causing ATXN8OS/ATXN8 expansions is higher than the disease because of the reduced penetrance of the expanded allele...
February 22, 2017: Acta Neurologica Scandinavica
https://www.readbyqxmd.com/read/28229452/expression-levels-of-transcription-factor-pu-1-and-interleukin-9-in-atopic-dermatitis-and-their-relation-to-disease-severity-and-eruption-types
#20
Ashraf M Hamza, Salma S Omar, Reham A H Abo El-Wafa, Marwa J Elatrash
BACKGROUND: The role of immunological factors in atopic dermatitis (AD) pathogenesis is well established. T-helper (TH) cells are central in AD pathogenesis. A relatively new subset of T cells, Th9 cells, was shown to be involved in the development of allergic asthma and allergic rhinitis, while its role in AD is still to be investigated. This study aimed to measure gene expression levels of interleukin-9 (IL-9) and PU.1, and to examine relationships with disease severity, serum IgE, and eruption types in AD patients...
February 22, 2017: International Journal of Dermatology
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