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https://www.readbyqxmd.com/read/29935420/inducement-of-a-spontaneously-wrinkled-polydimethylsiloxane-surface-and-its-potential-as-a-cell-culture-substrate
#1
Da Som Kim, Ho Won Lee, Jong Hyun Lee, Hyuck Gi Kwon, Sang Wook Lee, Seung Jin Han, Ok Chan Jeong
Spontaneous wrinkling of a polydimethylsiloxane (PDMS) surface was induced by repeated thermal shrinkage of liquid PDMS coated onto a cured PDMS layer. We investigated and evaluated the potential of the resulting surface as a cell culture substrate by monitoring the viability, spreading area, and proliferation rate of MG-63 cells cultured on native, wrinkled, and poly-L-lysine (PLL)-coated PDMS surfaces. Cells seeded on the wrinkled and PLL-coated PDMS surfaces spread and adhered better than those on native surfaces...
June 18, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29935331/prevalence-of-colistin-resistance-gene-mcr-1-in-colistin-resistant-escherichia-coli-and-klebsiella-pneumoniae-isolated-from-humans-in-thailand
#2
Warawan Eiamphungporn, Sakda Yainoy, Chakornpat Jumderm, Rachanis Tan-Arsuwongkul, Surapee Tiengrim, Visanu Thamlikitkul
OBJECTIVES: Colistin has historically been considered a last-line therapeutic option against multidrug-resistant (MDR) Gram-negative bacterial infections. However, chromosomally-encoded and plasmid-mediated colistin resistance is increasingly being reported worldwide. The spread of the plasmid-borne colistin resistance gene mcr-1 is of great concern since it can be transferred among bacteria. The aim of this study was to investigate the prevalence of colistin resistance gene mcr-1 in E...
June 20, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29935272/microrna-profiles-in-serum-samples-from-patients-with-stable-cirrhosis-and-mirna-21-as-a-predictor-of-transplant-free-survival
#3
Alex Evangelista do Amaral, Michele Patrícia Rode, Julia Cisilotto, Telma Erotides da Silva, Josiane Fischer, Camila Matiollo, Elayne Cristina de Morais Rateke, Janaína Luz Narciso-Schiavon, Leonardo Lucca Schiavon, Tânia Beatriz Creczynski-Pasa
MicroRNAs (miRNAs) have remarkable potential as diagnostic and prognostic markers because of their roles in disease pathogenesis. miRNAs can be released into the bloodstream, where they are sufficiently stable to be detected noninvasively. Here, we prospectively evaluated serum levels of miR-21, miR-34a, miR-122, miR-181b, and miR-885-5p in patients with stable cirrhosis. Total RNA was extracted from the sera of patients with cirrhosis and healthy individuals, and the expression levels of the target miRNAs were analyzed by reverse transcription-quantitative polymerase chain reaction...
June 20, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29935100/genetic-polymorphisms-of-the-hepatic-pathways-of-fatty-liver-disease-after-living-donor-liver-transplantation
#4
King-Wah Chiu, Shigeru Goto, Toshiaki Nakano, Tsung-Hui Hu, Ding-Wei Chen, Kuang-Tzu Huang, Li-Wen Hsu, Chao-Long Chen
BACKGROUND & AIMS: Fatty liver disease (FLD) is an important complication associated with liver transplantation, and the cytochrome P-450 system of the donor liver may be involved in its pathogenesis. To explore the effects of the CYP27A1, CYP27B1, CYP2R1, and vitamin D receptor (VDR) pathways on vitamin D maintenance after living donor liver transplantation (LDLT), we investigated the interplay between serum 25(OH)D and common variants in 60 paired donors and recipients who underwent LDLT...
June 23, 2018: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/29935069/il-1%C3%A9-c376a-transversion-variant-and-risk-of-idiopathic-male-infertility-in-iranian-men-a-genetic-association-study
#5
Tayyebeh Zamani-Badi, Mohammad Karimian, Abolfazl Azami Tameh, Hossein Nikzad
BACHGROUND: IL-1α produced by Sertoli cells is considered to act as a growth factor for spermatogonia. In this study, we investigated the association of the C376A polymorphism in IL-1α with male infertility in men referring to the Kashan IVF Center. MATERIALS AND METHODS: In this case-control study, 2 ml of blood was collected from 230 fertile and 230 infertile men. After DNA extraction, the C376A variant was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)...
June 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29935068/hypothalamic-kiss1-gpr54-gene-expressions-and-luteinizing-hormone-plasma-secretion-in-morphine-treated-male-rats
#6
Homayoun Khazali, Fariba Mahmoudi, Mahyar Janahmadi
BACHGROUND: The inhibitory effects of morphine and the stimulatory influence of kisspeptin signaling have been demonstrated on gonadotropin releasing hormone (GnRH)/luteinizing hormone (LH) release. Hypothalamic kisspeptin is involved in relaying the environmental and metabolic information to reproductive axis. In the present study, the role of kisspeptin/ GPR54 signaling system was investigated on relaying the inhibitory influences of morphine on LH hormone secretion. MATERIALS AND METHODS: In this experimental study, 55 wistar male rats weighing 230-250 g were sub-grouped in 11 groups (in each group n=5) receiving saline, kisspeptin (1 nmol), peptide234 (P234, 1 nmol), morphine (5 mg/kg), naloxone (2 mg/kg), kisspeptin/P234, morphine/naloxone, kisspeptin/morphine, kisspeptin/naloxone, P234/morphine or P234/naloxone respectively...
June 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29935063/prevalence-of-chlamydia-trachomatis-in-pregnant-iranian-women-a-systematic-review-and-meta-analysis
#7
Milad Azami, G Holamreza Badfar, Akram Mansouri, Mohammad Hossein Yekta Kooshali, Wesam Kooti, Zeinab Tardeh, Ali Soleymani, S Hamsi Abbasalizadeh
Several studies have been conducted regarding the prevalence of Chlamydia trachomatis, Mycoplasma hominis, and Ureaplasma urealyticum in pregnant Iranian women. However, it is necessary to combine the previous results to present a general assessment. We conducted the present study based on systematic review and meta-analysis studies according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). We searched the national and international online databases of MagIran, IranMedex, SID, MedLib, IranDoc, Scopus, PubMed, ISI Web of Knowledge, and Google Scholar search engine for certain MeSH keywords until June 16, 2017...
June 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29935018/a-novel-long-noncoding-rna-lncrna-ll22nc03-n64e9-1-promotes-the-proliferation-of-lung-cancer-cells-and-is-a-potential-prognostic-molecular-biomarker-for-lung-cancer
#8
Hongyu Jing, Xinglong Qu, Lingyun Liu, Huan Xia
BACKGROUND The aim of this study was to investigate the expression of a novel long noncoding RNA (lncRNA), LL22NC03-N64E9.1, and its effect on the phenotype of lung cancer cells and tissues using The Cancer Genome Atlas (TCGA) RNA sequencing data and other publicly available profiling data. MATERIAL AND METHODS The lung cancer dataset GSE30219 was downloaded from the Gene Expression Omnibus (GEO) repository. Differentially expressed lncRNA, LL22NC03-N64E9.1, in 48 lung cancer tissue samples and adjacent normal lung tissues, normal lung cell lines BEAS-2B and A549, and lung cancer cell lines, H1703, and H292, were detected by quantitative reverse transcription polymerase chain reaction (PCR) (qRT-PCR)...
June 23, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29934934/gene-profiling-involved-in-fate-determination-of-salivary-gland-type-in-mouse-embryogenesis
#9
Nirpesh Adhikari, Sanjiv Neupane, Jiyeon Roh, Yam Prasad Aryal, Eui-Seon Lee, Jae-Kwang Jung, Hitoshi Yamamoto, Youngkyun Lee, Wern-Joo Sohn, Jae-Young Kim, Ji-Youn Kim
Salivary gland (SG) development involves dynamic epithelial-mesenchymal interactions resulting in the formation of highly branched epithelial structures that produce and secrete saliva. The SG epithelium differentiates into saliva-producing terminal buds, i.e., acini, and transporting ducts. Most studies on the salivary gland have focused on branching morphogenesis; however, acinar cell differentiation underlying the determination of serous or mucous salivary glands is unclear. The objective of this study was to identify the mesenchymal signaling molecules involved in the epithelial differentiation of the salivary gland type as serous or mucous...
June 22, 2018: Genes & Genomics
https://www.readbyqxmd.com/read/29934882/molecular-and-immnune-diagnosis-further-testing-for-human-strongyloidiasis
#10
Larissa R Bosqui, Priscilla D Marques, Gessica B de Melo, Maria do Rosário F Gonçalves-Pires, Fernanda M Malta, Wander R Pavanelli, Ivete Conchon-Costa, Julia M Costa-Cruz, Fabiana M Paula, Idessania N Costa
INTRODUCTION: Detection of Strongyloides stercoralis larvae is particularly challenging because only a small number of larvae are released into the feces, regardless of infection stage. OBJECTIVE: Our objective was to apply conventional polymerase chain reaction (PCR) to the detection of S. stercoralis DNA in feces samples to evaluate its performance in samples of patients with strongyloidiasis and compare results with those of immunodiagnosis. METHODS: Stool, serum, and saliva samples were collected from each individual (n = 48) at the clinic hospital of the State University of Londrina, Brazil, for parasitological, immunological, and molecular tests...
June 21, 2018: Molecular Diagnosis & Therapy
https://www.readbyqxmd.com/read/29934881/streptococcus-mutans-in-umbilical-cord-blood-peripheral-blood-and-saliva-from-healthy-mothers
#11
Marcelly Milhomem Mendes, Camilla Beatriz da Silva, Denise Bertulucci Rocha Rodrigues, Barbara Rocha Rodrigues, Vinicius Rangel Geraldo-Martins, Virginia Paes Leme Ferriani, Virmondes Rodrigues, Ruchele Dias Nogueira
The aims of this study were to analyze the presence of Streptococcus mutans (SM)-DNA in cord blood (CB), maternal peripheral blood (PB), and maternal saliva (SA) and compare with data collected in health surveys. Sixty-four healthy women with pregnancies to term and without complications attending for elective cesarean section in the Clinical Hospital of Ribeirao Preto, Sao Paulo were included. Samples of PB and unstimulated SA were obtained on the day of hospitalization and samples of CB were collected after the delivery section...
June 22, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29934812/differential-expression-of-primary-pair-rule-genes-during-bidirectional-regeneration-in-perionyx-excavatus
#12
Yun-Sang Yu, Jin-Se Kim, Brenda Irene Medina Jiménez, Tae-Wuk Kim, Sung-Jin Cho
The earthworm Perionyx excavatus is a species highly capable of bidirectional regeneration. Pair-rule genes are thought to have an ancestral function in arthropod segmentation. However, orthologs in annelids (i.e. Capitella teleta) do not exhibit segmental expression in the ectoderm or mesoderm. Their role in regeneration is currently unclear. Here, we report the expression profile of primary pair-rule genes (Pex-EvxA, Pex-EvxB, Pex-RuntA, Pex-RuntB, Pex-Hes1A, Pex-Hes1B, Pex-Hes4A, and Pex-Hey) found in P...
July 2018: Genes & Genomics
https://www.readbyqxmd.com/read/29934690/knockdown-of-microrna-21-promotes-neurological-recovery-after-acute-spinal-cord-injury
#13
Wei Xie, Shang-You Yang, Qianqian Zhang, Yadong Zhou, Yi Wang, Ronghan Liu, Wenzhao Wang, Jixue Shi, Bin Ning, Tanghong Jia
To assess the therapeutic effects of microRNA-21 (miR-21) knockdown (KD) for acute thoracic spinal cord contusion using a mouse model. Forty C57/BL6 mice were randomly divided into four groups: mice in the sham-operated (Sham) group received surgical procedure without spinal cord contusion; the spinal cord injury (SCI) group mice underwent spinal cord contusion without treatment; mice in the miR-21 KD group underwent spinal cord contusion followed by a single dose subdural injection of miR-21 KD vectors (1 × 107 TU); and the negative control (NC) group mice were given subdural injection of comparable amount of NC vectors (1 × 107 TU) after spinal cord contusion...
June 22, 2018: Neurochemical Research
https://www.readbyqxmd.com/read/29934515/localization-and-density-of-porphyromonas-gingivalis-and-tannerella-forsythia-in-gingival-and-subgingival-granulation-tissues-affected-by-chronic-or-aggressive-periodontitis
#14
G Amodini Rajakaruna, Mariko Negi, Keisuke Uchida, Masaki Sekine, Asuka Furukawa, Takashi Ito, Daisuke Kobayashi, Yoshimi Suzuki, Takumi Akashi, Makoto Umeda, Walter Meinzer, Yuichi Izumi, Yoshinobu Eishi
Porphyromonas gingivalis and Tannerella forsythia have been thought to be associated with periodontitis; however comprehensive histopathological localization of bacteria in affected human periodontal tissues is not well documented. In the present study, we examined formalin-fixed paraffin-embedded gingival and subgingival granulation tissues from 71 patients with chronic periodontitis and 11 patients with aggressive periodontitis, using immunohistochemistry with novel monoclonal antibodies specific to P. gingivalis or T...
June 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29934504/quantitative-assessment-of-individual-populations-within-polymicrobial-biofilms
#15
Susana Patrícia Lopes, Nuno Filipe Azevedo, Maria Olívia Pereira
Selecting appropriate tools providing reliable quantitative measures of individual populations in biofilms is critical as we now recognize their true polymicrobial and heterogeneous nature. Here, plate count, quantitative real-time polymerase chain reaction (q-PCR) and peptide nucleic acid probe-fluorescence in situ hybridization (PNA-FISH) were employed to quantitate cystic fibrosis multispecies biofilms. Growth of Pseudomonas aeruginosa, Inquilinus limosus and Dolosigranulum pigrum was assessed in dual- and triple-species consortia under oxygen and antibiotic stress...
June 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29934271/intermediate-length-cag-repeat-in-atxn2-is-associated-with-increased-risk-for-amyotrophic-lateral-sclerosis-in-brazilian-patients
#16
Helen Maia Tavares de Andrade, Vívian Pedigone Cintra, Milena de Albuquerque, Camila Callegari Piccinin, Luciana Cardoso Bonadia, Rafael Esteves Duarte Couteiro, Daniel Sabino de Oliveira, Rinaldo Claudino, Marcos Vinicius Magno Gonçalves, Mario Emilio Teixeira Dourado, Leonardo Cruz de Souza, Antônio Lúcio Teixeira, Laura de Godoy Rousseff Prado, Vitor Tumas, Acary Souza Bulle Oliveira, Anamarli Nucci, Iscia Lopes-Cendes, Wilson Marques, Marcondes C França
Intermediate-length cytosine-adenine-guanine nucleotide repeat expansions in the ATXN2 gene (which encodes for the protein Ataxin-2) have been linked to increased risk for amyotrophic lateral sclerosis (ALS) in different populations. There is no such study in the Brazilian population, which has a mixed ethnic background. We have thus selected 459 patients with ALS (372 Sporadic ALS and 87 Familial ALS) and 468 control subjects from 6 Brazilian centers to investigate this point. We performed polymerase chain reaction to determine the length of the ATXN2 alleles...
May 8, 2018: Neurobiology of Aging
https://www.readbyqxmd.com/read/29934114/genotypes-and-phenotypes-in-20-chinese-patients-with-type-2-gaucher-disease
#17
Lulu Kang, Yu Wang, Xiaolan Gao, Wenjuan Qiu, Jun Ye, Lianshu Han, Xuefan Gu, Huiwen Zhang
BACKGROUND: Gaucher disease (GD) is one of the most common lysosomal storage diseases resulting from a deficiency of glucocerebrosidase. Three main types have been described, with type 2 being the most rare and severe form. Here we investigated the clinical symptoms and mutation spectrum in 20 unrelated type 2 GD patients. METHOD: The diagnosis of GD was based on the acid β-glucocerebrosidase (GBA) enzyme activity and direct sequencing of the GBA gene. GBA activity was measured in leukocytes and the GBA gene was amplified by a polymerase chain reaction (PCR)...
June 19, 2018: Brain & Development
https://www.readbyqxmd.com/read/29934103/personalising-treatment-for-high-grade-serous-ovarian-carcinoma
#18
E Cojocaru, C A Parkinson, J D Brenton
Ovarian cancer is a heterogeneous group of cancers that differ by cell of origin and genomic features. High-grade serous ovarian cancer (HGSOC) is the commonest histiotype and is characterized by extreme genomic complexity and dysregulation of DNA damage repair pathways, particularly homologous recombination deficiency. New insights from molecular profiling into homologous recombination deficiency now offers the credible possibility of personalizing treatment choices for women with HGSOC using poly(ADP-ribose) polymerase inhibitor (PARP) therapy...
June 19, 2018: Clinical Oncology: a Journal of the Royal College of Radiologists
https://www.readbyqxmd.com/read/29933737/-genotyping-of-echinococcus-granulosus-isolates-by-sequencing-of-mitochondrial-cytochrome-c-oxidase-subunit-1-cox1-gene-in-aydin
#19
Aylin Oral Babaoğlu, Aykut Soyder, Erdoğan Malatyalı, Sema Ertuğ, Hatice Ertabaklar
Cyst hydatid (CH) is a zoonotic infection that is characterized by the development of metacestode form of Echinococcus granulosus primarily in liver of humans and ruminants. With a worldwide distribution, the infection is still considered as an important parasitic disease that threatens the public health in Turkey as in the other developing countries. Morphological and biological features of parasite fail to discriminate isolates for typing so molecular methods should be used for this purpose. Recently, a total of eleven genotypes of E...
April 2018: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29933733/-rotavirus-prevalence-and-genotype-distribution-in-children-with-acute-gastroenteritis-in-adana-province
#20
Özlem Özgür Gündeşlioğlu, Emine Kocabaş, Zeliha Haytoğlu, Gülperi Timurtaş Dayar, Merve Kılıç Çil, Rıza Durmaz
Rotavirus is the most common agent of acute gastroenteritis in children under five years of age and still a major cause of mortality and morbidity mainly in devoloping countries. The aim of this study was to determine the circulating rotavirus genotypes in children less than five years of age with acute gastroenteritis in Adana province, before the introduction of the rotavirus vaccine into the national immunization programme. Fecal specimens were collected from children enrolled between December 2012 and November 2016 and rotavirus antigen was searched by a rapid immunochromatographic test method...
April 2018: Mikrobiyoloji Bülteni
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