Ana-Mishel Spiroski, Ian R McCracken, Adrian Thomson, Marlene Magalhaes-Pinto, Mukesh K Lalwani, Kathryn J Newton, Eileen Miller, Cecile Bénézech, Patrick Hadoke, Mairi Brittan, Joanne C Mountford, Abdelaziz Beqqali, Gillian A Gray, Andrew H Baker
Background: Mechanisms contributing to tissue remodeling of the infarcted heart following cell-based therapy remain elusive. While cell-based interventions have the potential to influence the cardiac healing process, there is little direct evidence of preservation of functional myocardium. Aim: The aim of the study was to investigate tissue remodeling in the infarcted heart following human embryonic stem cell-derived endothelial cell product (hESC-ECP) therapy. Materials and methods: Following coronary artery ligation (CAL) to induce cardiac ischemia, we investigated infarct size at 1 day post-injection in media-injected controls (CALM, n = 11), hESC-ECP-injected mice (CALC, n = 10), and dead hESC-ECP-injected mice (CALD, n = 6); echocardiography-based functional outcomes 14 days post-injection in experimental (CALM, n = 13; CALC, n = 17) and SHAM surgical mice ( n = 4); and mature infarct size (CALM and CALC, both n = 6)...
2022: Frontiers in Cardiovascular Medicine