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https://www.readbyqxmd.com/read/28550199/comprehensive-approach-for-identifying-the-t-cell-subset-origin-of-cd3-and-cd28-antibody-activated-chimeric-antigen-receptor-modified-t-cells
#1
Michael Schmueck-Henneresse, Bilal Omer, Thomas Shum, Haruko Tashiro, Maksim Mamonkin, Natalia Lapteva, Sandhya Sharma, Lisa Rollins, Gianpietro Dotti, Petra Reinke, Hans-Dieter Volk, Cliona M Rooney
The outcome of therapy with chimeric Ag receptor (CAR)-modified T cells is strongly influenced by the subset origin of the infused T cells. However, because polyclonally activated T cells acquire a largely CD45RO(+)CCR7(-) effector memory phenotype after expansion, regardless of subset origin, it is impossible to know which subsets contribute to the final T cell product. To determine the contribution of naive T cell, memory stem T cell, central memory T cell, effector memory T cell, and terminally differentiated effector T cell populations to the CD3 and CD28-activated CAR-modified T cells that we use for therapy, we followed the fate and function of individually sorted CAR-modified T cell subsets after activation with CD3 and CD28 Abs (CD3/28), transduction and culture alone, or after reconstitution into the relevant subset-depleted population...
May 26, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28549260/identification-of-oral-cavity-biofilm-forming-bacteria-and-determination-of-their-growth-inhibition-by-acacia-arabica-tamarix-aphylla-l-and-melia-azedarach-l-medicinal-plants
#2
Muhammad Khalid, Danial Hassani, Muhammad Bilal, Zahid Ali Butt, Muhammad Hamayun, Ayaz Ahmad, Danfeng Huang, Anwar Hussain
OBJECTIVE: Biofilms are complex, multi-species bacterial communities that colonize the oral cavity in the form of plaque and are known to cause dental caries and periodontal disease. Present study demonstrated the potential of three selected medicinal plants against isolated and identified dental biofilm forming strains. METHODS: Pathogenic bacteria from dental biofilms were isolated, cultured, identified by phylogenetic analysis using PCR-based 16S ribosomal RNA (or 16S rRNA) nucleotide sequences and were analyzed for their biofilm forming capability...
May 18, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28546894/needs-and-challenges-for-assessing-the-environmental-impacts-of-engineered-nanomaterials-enms
#3
REVIEW
Michelle Romero-Franco, Hilary A Godwin, Muhammad Bilal, Yoram Cohen
The potential environmental impact of nanomaterials is a critical concern and the ability to assess these potential impacts is top priority for the progress of sustainable nanotechnology. Risk assessment tools are needed to enable decision makers to rapidly assess the potential risks that may be imposed by engineered nanomaterials (ENMs), particularly when confronted by the reality of limited hazard or exposure data. In this review, we examine a range of available risk assessment frameworks considering the contexts in which different stakeholders may need to assess the potential environmental impacts of ENMs...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28546088/extracorporeal-shock-wave-lithotripsy-management-of-renal-stones-in-children-does-anesthesia-affect-the-treatment-outcomes-on-an-age-based-manner
#4
Murat Tuncer, Banu Çevik, Bilal Eryıldırım, Gökhan Faydacı, Kemal Sarıca
OBJECTIVE: To evaluate beneficial effect of anaesthesia on extracorporeal shock wave lithotripsy (SWL) in children older than nine years. PATIENTS AND METHODS: A total of 61 children (aging between 9-17 years) treated with SWL for renal stones were included. Depending on the use of general anesthesia, children were divided into two groups; (Group 1, n=27) Cases treated with SWL under anesthesia and (Group 2, n=34) SWL without anesthesia. Patients in both groups were comparatively evaluated with respect to treatment related (stone size, stone laterality, stone location, mean number of sessions, mean number of shock waves applied, residual fragments size, stone free rate (SFR), clinically significant residual fragment complications and need of additional intervention) parameters...
May 22, 2017: Urology
https://www.readbyqxmd.com/read/28545151/provenance-based-data-integrity-checking-and-verification-in-cloud-environments
#5
Muhammad Imran, Helmut Hlavacs, Inam Ul Haq, Bilal Jan, Fakhri Alam Khan, Awais Ahmad
Cloud computing is a recent tendency in IT that moves computing and data away from desktop and hand-held devices into large scale processing hubs and data centers respectively. It has been proposed as an effective solution for data outsourcing and on demand computing to control the rising cost of IT setups and management in enterprises. However, with Cloud platforms user's data is moved into remotely located storages such that users lose control over their data. This unique feature of the Cloud is facing many security and privacy challenges which need to be clearly understood and resolved...
2017: PloS One
https://www.readbyqxmd.com/read/28542353/identification-of-cow-and-buffalo-milk-based-on-beta-carotene-and-vitamin-a-concentration-using-fluorescence-spectroscopy
#6
Rahat Ullah, Saranjam Khan, Hina Ali, Muhammad Bilal, Muhammad Saleem
The current study presents the application of fluorescence spectroscopy for the identification of cow and buffalo milk based on β-carotene and vitamin-A which is of prime importance from the nutritional point of view. All samples were collected from healthy animals of different breeds at the time of lactation in the vicinity of Islamabad, Pakistan. Cow and buffalo milk shows differences at fluorescence emission appeared at band position 382 nm, 440 nm, 505 nm and 525 nm both in classical geometry (right angle) setup as well as front face fluorescence setup...
2017: PloS One
https://www.readbyqxmd.com/read/28540554/understanding-brassinosteroid-regulated-mechanisms-to-improve-stress-tolerance-in-plants-a-critical-review
#7
Fahim Nawaz, Muhammad Naeem, Bilal Zulfiqar, Asim Akram, Muhammad Yasin Ashraf, Muhammad Raheel, Rana Nauman Shabbir, Rai Altaf Hussain, Irfan Anwar, Muhammad Aurangzaib
Brassinosteroids (BRs) are steroidal plant hormones involved in regulation of physiological and molecular processes to ameliorate various biotic and abiotic stresses. Exogenous application of BRs to improve stress tolerance in plants has recently become a high research priority. Several studies have revealed the involvement of these steroidal hormones in upregulation of stress-related defense genes and their cross talk with other metabolic pathways. This is likely to stimulate research on many unanswered questions regarding their role in enhancing the ability of plants to tolerate adverse environmental conditions...
May 24, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28536726/anaemia-with-laugier-hunziker-syndrome-a-diagnostic-dilemma
#8
Muhammad Bilal Abid, Paras Mughal, Muhammad Abbas Abid
No abstract text is available yet for this article.
May 2017: Singapore Medical Journal
https://www.readbyqxmd.com/read/28536224/impella-percutaneous-left-ventricular-assist-device-for-severe-acute-ischaemic-mitral-regurgitation-as-a-bridge-to-surgery
#9
Bilal Jalil, Karim El-Kersh, Jarrod Frizzell, Shozab Ahmed
Ischaemic papillary muscle rupture causing acute severe mitral regurgitation (MR) has a dramatic presentation and a very high mortality. Emergent surgical repair improves outcomes, which necessitates robust preoperative stabilisation. Here we discuss a patient with cardiogenic shock with an acute severe MR that was deemed very high risk for emergent valve replacement due to haemodynamic instability and respiratory failure. A percutaneous left ventricular assist device Impella 2.5 (Abiomed, Danvers, MA) drastically improved clinical status, and the patient underwent a successful surgical mitral valve replacement soon after placement of the temporary assist device...
May 22, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28535199/genotype-phenotype-correlation-in-patients-with-isovaleric-acidemia-comparative-structural-modelling-and-computational-analysis-of-novel-variants
#10
Osama K Zaki, George Priya Doss C, Salsabil A Ali, Ghadeer G Murad, Shaima A Elashi, Maryam Sa Ebnou, D Thirumal Kumar, Ola Khalifa, Radwa Gamal, Heba S A El Abd, Bilal N Nasr, Hatem Zayed
Isovaleric acidemia (IVA) is an autosomal recessive inborn error of leucine metabolism. It is caused by a deficiency in the mitochondrial isovaleryl-CoA dehydrogenase (IVD) enzyme. In this study, we investigated eight patients with IVA. The patients diagnoses were confirmed by urinary organic acid analysis and the blood C5-Carnitine value. A molecular genetic analysis of the IVD gene revealed nine different variants: five were missense variants (c.1193G>A; p. R398Q, c.1207T>A; p. Y403N, c.872C>T; p...
May 23, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28528940/macromolecular-agents-with-antimicrobial-potentialities-a-drive-to-combat-antimicrobial-resistance
#11
REVIEW
Muhammad Bilal, Tahir Rasheed, Hafiz M N Iqbal, Hongbo Hu, Wei Wang, Xuehong Zhang
In recent years, the antimicrobial resistance (AMR) or multidrug resistance (MDR) has become a serious health concern and major challenging issue, worldwide. After decades of negligence, the AMR has now captured global attention. The increasing number of antibiotic-resistant strains has threatened the achievements of science and medicine since it inactivates conventional antimicrobial therapeutics. Scientists are trying to respond to AMR/MDR threat by exploring innovative platforms and new therapeutic strategies to tackle infections from these resistant strains and bypass treatment limitations related to these pathologies...
May 19, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28528290/modelling-lung-cancer-mortality-rates-from-smoking-prevalence-fill-in-the-gap
#12
Juan Carlos Martín-Sánchez, Usama Bilal, Ramon Clèries, Cristina Lidón-Moyano, Marcela Fu, Luís González-de Paz, Manuel Franco, Esteve Fernandez, Jose M Martínez-Sánchez
BACKGROUND: The objective of this study is to estimate the gap between smoking prevalence and lung cancer mortality and provide predictions of lung cancer mortality based on previous smoking prevalence. MATERIALS AND METHODS: We used data from the Spanish National Health Surveys (2003, 2006 and 2011) to obtain information about tobacco use and data from the Spanish National Statistics Institute to obtain cancer mortality rates from 1980 to 2013. We calculated the cross-correlation among the historical series of smoking prevalence and lung cancer mortality rate (LCMR) to estimate the most likely time gap between both series...
May 18, 2017: Cancer Epidemiology
https://www.readbyqxmd.com/read/28527973/molecular-analysis-of-group-a-rotaviruses-detected-in-hospitalized-children-from-rawalpindi-pakistan-during-2014
#13
Massab Umair, Bilal Haider Abbasi, Nadia Nisar, Muhammad Masroor Alam, Salmaan Sharif, Shahzad Shaukat, Muhammad Suleman Rana, Adnan Khurshid, Ghulam Mujtaba, Uzma Bashir Aamir, Syed Sohail Zahoor Zaidi
As part of strategy to control diarrheal diseases, World Health Organization (WHO) recommends to include rotavirus vaccines in national immunization programs. Sentinel surveillance networks have been established to monitor rotavirus disease burden and genotype distribution in both pre and post vaccine era in many countries. Unfortunately due to lack of proper surveillance programs, data on rotavirus disease burden and genotype distribution from Pakistan is scarce. We investigated 502 stool samples from children (<5years) hospitalized due to gastroenteritis in Rawalpindi, Pakistan during 2014 for the presence of group A rotavirus (RVA) and its genotypic diversity...
May 17, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28526660/magnetic-nanoformulations-for-prostate-cancer
#14
Pallabita Chowdhury, Allison M Roberts, Sheema Khan, Bilal B Hafeez, Subhash C Chauhan, Meena Jaggi, Murali M Yallapu
Magnetic nanoparticles (MNPs) play a vital role for improved imaging applications. Recently, a number of studies demonstrate MNPs can be applied for targeted delivery, sustained release of therapeutics, and hyperthermia. Based on stable particle size and shape, biocompatibility, and inherent contrast enhancement characteristics, MNPs have been encouraged for pre-clinical studies and human use. As a theranostic platform development, MNPs need to balance both delivery and imaging aspects. Thus, this review provides significant insight and advances in the theranostic role of MNPs through the documentation of unique magnetic nanoparticles used in prostate cancer, their interaction with prostate cancer cells, in vivo fate, targeting, and biodistribution...
May 16, 2017: Drug Discovery Today
https://www.readbyqxmd.com/read/28526021/current-status-challenges-and-the-way-forward-for-clinical-pharmacy-service-in-ethiopian-public-hospitals
#15
Arebu Issa Bilal, Zelalem Tilahun, Gebremedhin Beedemariam Gebretekle, Belete Ayalneh, Bisrat Hailemeskel, Ephrem Engidawork
BACKGROUND: Clinical pharmacy service has evolved steadily over the past few decades and is now contributing to the 'patient care journey' at all stages. It is improving the safety and effectiveness of medicines and has made a significant contribution to the avoidance of medication errors. In Ethiopia, clinical pharmacy service is in its initial phase, being started in July 2013. This study therefore aimed at assessing the status, challenges and way forward of clinical pharmacy service in the country...
May 19, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28523052/efficacy-of-brow-suspension-with-autogenous-fascia-lata-in-simple-congenital-ptosis
#16
Farhan Ali, Muhammad Saim Khan, Muhammad Sharjeel, Zaheer Ud Din, Bilal Murtaza, Asfandyar Khan
OBJECTIVE: To assess the mean change in interpalpebral fissure height and marginal reflex distance after brow suspension with autogenous fascia lata sling in patients of ptosis. METHODS: This was a Quasi experimental study conducted at Department of Ophthalmology, Mayo Hospital, King Edwards Medical University Lahore, from Jan 2013 to June 2016. Included were the patients who had unilateral or bilateral ptosis with poor levator function (< 5 mm). Informed consent was obtained from all patients after explaining about the research project...
March 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28523037/frequency-of-dyslipidemia-in-patients-with-lupus-nephritis
#17
Saba Sajjad, Sumaira Farman, Muhammad Ahmed Saeed, Nighat Mir Ahmad, Bilal Azeem Butt
OBJECTIVE: To determine the frequency of dyslipidemia in patients with lupus nephritis and its association with the degree of proteinuria. METHODS: This cross-sectional analytic study included 65 patients who fulfilled the ACR (American College of Rheumatology) criteria for SLE and had renal involvement, presenting to the Division of Rheumatology, Fatima Memorial Hospital (FMH), and Lahore from 21(st) Sep 2016 to 20(th) Dec 2016. After 12 hours overnight fast their blood samples were assessed for total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL)...
March 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28523031/myocardial-protection-with-glucose-insulin-potassium-infusion-during-adult-cardiac-surgery
#18
Suhail Ahmad, Rana Altaf Ahmad, Bilal Ahsan Qureshi, Mirza Ahmad Raza Baig
BACKGROUND & OBJECTIVE: Recent meta-analysis reports have called for more randomized trials to evaluate the effectiveness of GIK solution in patients of cardiac surgery. So this study was conducted to evaluate the effectiveness of Glucose-insulin-potassium (GIK) solutions in non-diabetic patients undergoing coronary artery bypass grafting. METHODS: A total number of one hundred and sixty (160) patients were randomized into two equal groups; GIK Group and non-GIK group...
March 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28522177/re-lower-urinary-tract-symptoms-and-incident-falls-in-community-dwelling-older-men-the-concord-health-and-ageing-in-men-project
#19
Dominique Thomas, Bilal Chughtai
No abstract text is available yet for this article.
May 15, 2017: European Urology
https://www.readbyqxmd.com/read/28516112/hemorrhagic-cystitis-in-a-liver-transplant-recipient-secondary-to-bk-virus
#20
Faisal Kamal, Bilal Ali, Matthew Barnes, Sehrish Kamal, Satheesh Nair, Humberto Gonzalez
The association between BK virus infection and hemorrhagic cystitis (HC) in hematopoietic stem cell transplant (HSCT) recipients is well established. However, BK virus-associated HC has not been described in liver transplant (LT) recipients. We present a case of BK virus-associated HC in a LT recipient. Our patient presented with worsening liver function tests 2 years after transplantation and was found to have acute cellular rejection. He was treated with increased immunosuppression and subsequently developed hematuria...
2017: ACG Case Reports Journal
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