Roberto Toni, Fulvio Barbaro, Giusy Di Conza, Nicoletta Zini, Giulia Remaggi, Lisa Elviri, Giulia Spaletta, Enrico Quarantini, Marco Quarantini, Salvatore Mosca, Silvio Caravelli, Massimiliano Mosca, Francesca Ravanetti, Simone Sprio, Anna Tampieri
We engineered an in vitro model of bioartificial 3D bone organoid consistent with an anatomical and vascular microenvironment common to mammalian flat and short bones. To achieve this, we chose the decellularized-decalcified matrix of the adult male rat scapula, implemented with the reconstruction of its intrinsic vessels, obtained through an original intravascular perfusion with polylevolactic (PLLA), followed by coating of the PLLA-fabricated vascularization with rat tail collagen. As a result, the 3D bone and vascular geometry of the native bone cortical and cancellous compartments was reproduced, and the rat tail collagen-PLLA biomaterial could in vitro act as a surrogate of the perivascular extracellular matrix (ECM) around the wall of the biomaterial-reconstituted cancellous vessels...
October 29, 2023: Journal of Biomedical Materials Research. Part B, Applied Biomaterials