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Perfusion decellularization

Jun Pan, Sheng Yan, Jun-Jie Gao, Ying-Ying Wang, Zhong-Jie Lu, Chen-Wei Cui, Yao-Hui Zhang, Yan Wang, Xue-Qin Meng, Lin Zhou, Hai-Yang Xie, Jessica Zheng, Ming H Zheng, Shu-Sen Zheng
Organ decellularization is emerging as a promising regenerative medicine approach as it is able to provide an acellular, three-dimensional biological scaffold material that can be seeded with living cells for organ reengineering. However this application is currently limited to donor-derived decellularized organs for reengineering in vitro and no study has been conducted for re-engineering the decellularized organ in vivo. We developed a novel technique of a single liver lobe decellularization in vivo in live animals...
October 5, 2016: International Journal of Biochemistry & Cell Biology
Lindsey Dew, William R English, Chuh K Chong, Sheila MacNeil
One of the main challenges currently faced by tissue engineers is the loss of tissues post implantation due to delayed neovascularization. Several strategies are under investigation to create vascularized tissue but none have yet overcome this problem. In this study we produced a decellularized natural vascular scaffold from rat intestine to use as an in vitro platform for neovascularization studies for tissue engineered constructs. Decellularization resulted in almost complete (97%) removal of nuclei and DNA, while collagen, glycosaminoglycans and laminin content was preserved...
September 27, 2016: Tissue Engineering. Part A
Sarah E Gilpin, Jonathan M Charest, Xi Ren, Luis F Tapias, Tong Wu, Daniele Evangelista-Leite, Douglas J Mathisen, Harald C Ott
Bio-engineered organs for transplantation may ultimately provide a personalized solution for end-stage organ failure, without the risk of rejection. Building upon the process of whole organ perfusion decellularization, we aimed to develop novel, translational methods for the recellularization and regeneration of transplantable lung constructs. We first isolated a proliferative KRT5(+)TP63(+) basal epithelial stem cell population from human lung tissue and demonstrated expansion capacity in conventional 2D culture...
November 2016: Biomaterials
Franziska Mußbach, Utz Settmacher, Olaf Dirsch, Chichi Xie, Uta Dahmen
BACKGROUND: Organ engineering is a new innovative strategy to cope with two problems: the need for physiological models for pharmacological research and donor organs for transplantation. A functional scaffold is generated from explanted organs by removing all cells (decellularization) by perfusing the organ with ionic or nonionic detergents via the vascular system. Subsequently the acellular scaffold is reseeded with organ-specific cells (repopulation) to generate a functional organ. SUMMARY: This review gives an overview of the state of the art describing the decellularization process, the subsequent quality assessment, the repopulation techniques and the functional assessment...
July 27, 2016: European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
Shu-Wei Xiao, Peng-Chao Wang, Wei-Jun Fu, Zhong-Xin Wang, Gang Li, Xu Zhang
As the endoscopic technique is widely used in the diagnosis and treatment of diseases, the incidence of ureteral injuries increases annually. The classical surgical therapies are not always satisfactory. With the constant development of the tissue engineering technology in the field of urinary reconstruction, the ureteral reconstruction has become possible technology. In this study, a novel perfusion-decellularized protocol, which combined a perfusion system with the commonly used physical and chemical methods, was used to prepare the decellularized ureters for ureteral reconstruction and the urinary tract-derived cells (UDCs) were seeded on the surface of the perfusion-decellularized ureters (PDUs) in order to observe the cells survival, adhesion, proliferation and distribution...
July 9, 2016: Journal of Bioscience and Bioengineering
Yusuke Katsuki, Hiroshi Yagi, Teru Okitsu, Minoru Kitago, Kazuki Tajima, Yoshie Kadota, Taizo Hibi, Yuta Abe, Masahiro Shinoda, Osamu Itano, Shoji Takeuchi, Yuko Kitagawa
OBJECTIVES: Because therapeutic options for severe diabetes are currently limited, there is a continuing need for new therapeutic strategies, especially in the field of regenerative medicine. Collaborative efforts across the fields of tissue engineering technology and islet biology may be able to create functionally engineered islets capable of restoring endocrine function in patients with insulin-dependent diabetes. METHODS: This engineered scaffold was seeded with isolated primary porcine islets via the pancreatic duct using a multi-step infusion technique...
September 2016: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
Pedro L Sánchez, M Eugenia Fernández-Santos, M Angeles Espinosa, M Angeles González-Nicolas, Judith R Acebes, Salvatore Costanza, Isabel Moscoso, Hugo Rodríguez, Julio García, Jesús Romero, Stefan M Kren, Javier Bermejo, Raquel Yotti, Candelas Pérez Del Villar, Ricardo Sanz-Ruiz, Jaime Elizaga, Doris A Taylor, Francisco Fernández-Avilés
Perfusion decellularization of cadaveric hearts removes cells and generates a cell-free extracellular matrix scaffold containing acellular vascular conduits, which are theoretically sufficient to perfuse and support tissue-engineered heart constructs. This article contains additional data of our experience decellularizing and testing structural integrity and composition of a large series of human hearts, "Acellular human heart matrix: a critical step toward whole heat grafts" (Sanchez et al., 2015) [1]. Here we provide the information about the heart decellularization technique, the valve competence evaluation of the decellularized scaffolds, the integrity evaluation of epicardial and myocardial coronary circulation, the pressure volume measurements, the primers used to assess cardiac muscle gene expression and, the characteristics of donors, donor hearts, scaffolds and perfusion decellularization process...
September 2016: Data in Brief
Sarindr Bhumiratana, Jonathan C Bernhard, David M Alfi, Keith Yeager, Ryan E Eton, Jonathan Bova, Forum Shah, Jeffrey M Gimble, Mandi J Lopez, Sidney B Eisig, Gordana Vunjak-Novakovic
Facial deformities require precise reconstruction of the appearance and function of the original tissue. The current standard of care-the use of bone harvested from another region in the body-has major limitations, including pain and comorbidities associated with surgery. We have engineered one of the most geometrically complex facial bones by using autologous stromal/stem cells, native bovine bone matrix, and a perfusion bioreactor for the growth and transport of living grafts, without bone morphogenetic proteins...
June 15, 2016: Science Translational Medicine
Zhiyi Wang, Zhibin Wang, Qing Yu, Haitao Xi, Jie Weng, Xiaohong Du, Daqing Chen, Jianshe Ma, Jin Mei, Chan Chen
Decellularization processes may variably distort or degrade extracellular matrix (ECM) structure. In this study, two perfusion routes (PR) were tested on SD rat lung samples. One decellularization protocol, PR1, was perfused through the pulmonary artery. The other decellularization protocol, PR2, was perfused through the trachea. Both decellularization protocols were used by the same detergent-based (sodium dodecyl sulphate and Triton X-100) with different flow continuous perfusion. There was no visible difference in vessel architecture between PR1- and PR2-decellularized scaffold...
October 2016: Journal of Biomedical Materials Research. Part A
Fabian Koenig, Jang-Sun Lee, Bassil Akra, Trixi Hollweck, Erich Wintermantel, Christian Hagl, Nikolaus Thierfelder
Transcatheter aortic valve implantation (TAVI) is a fast-growing, exciting field of invasive therapy. During the last years many innovations significantly improved this technique. However, the prostheses are still associated with drawbacks. The aim of this study was to create cell-seeded biohybrid aortic valves (BAVs) as an ideal implant by combination of assets of biological and artificial materials. Furthermore, the influence of TAVI procedure on tissue-engineered BAV was investigated. BAV (n=6) were designed with decellularized homograft cusps and polyurethane walls...
August 2016: Artificial Organs
Florian Groeber, Lisa Engelhardt, Julia Lange, Szymon Kurdyn, Freia F Schmid, Christoph Rücker, Stephan Mielke, Heike Walles, Jan Hansmann
Tissue-engineered skin equivalents mimic key aspects of the human skin, and can thus be employed as wound coverage for large skin defects or as in vitro test systems as an alternative to animal models. However, current skin equivalents lack a functional vasculature limiting clinical and research applications. This study demonstrates the generation of a vascularized skin equivalent with a perfused vascular network by combining a biological vascularized scaffold (BioVaSc) based on a decellularized segment of a porcine jejunum and a tailored bioreactor system...
May 15, 2016: ALTEX
Sharon Geerts, Sinan Ozer, Maria Jaramillo, Martin L Yarmush, Basak E Uygun
Whole liver engineering holds the promise to create transplantable liver grafts that may serve as substitutes for donor organs, addressing the donor shortage in liver transplantation. While decellularization and recellularization of livers in animal models have been successfully achieved, scale up to human livers has been slow. There are a number of donor human livers that are discarded because they are not found suitable for transplantation, but are available for engineering liver grafts. These livers are rejected due to a variety of reasons, which in turn may affect the decellularization outcome...
July 2016: Tissue Engineering. Part C, Methods
Junxi Xiang, Xinglong Zheng, Peng Liu, Lifei Yang, Dinghui Dong, Wanquan Wu, Xuemin Liu, Jianhui Li, Yi Lv
BACKGROUND AND AIMS: Decellularized liver matrix (DLM) hold great potential for reconstructing functional hepatic-like tissue (HLT) based on reseeding of hepatocytes or stem cells, but the shortage of liver donors is still an obstacle for potential application. Therefore, an appropriate alternative scaffold is needed to expande the donor pool. In this study, we explored the effectiveness of decellularized spleen matrix (DSM) for culturing of bone marrow mesenchymal stem cells (BMSCs), and promoting differentiation into hepatic-like cells...
May 9, 2016: Organogenesis
Jian Zhang, Wen Yue Cheng, Zhi Qian Hu, Neill J Turner, Li Zhang, Qiang Wang, Stephen F Badylak
In this dataset, we particularly depicted the harvest and perfusion decellularization of porcine rectus abdominis (RA), accompanied with displaying of the retained vascular trees within the perfusion-decellularized skeletal muscle matrix (pM-ECM) using vascular corrosion casting. In addition, several important tips for successful pM-ECM preparation were emphasized, which including using anatomically isolated skeletal muscle as tissue source with all main feeding and draining vessels perfused, preserving the internal microcirculation availability, aseptic technique and pyrogen free in all steps, sequential perfusion via artery or vein, and longtime washing after decellularization...
June 2016: Data in Brief
Robert Ramm, Heiner Niemann, Björn Petersen, Axel Haverich, Andres Hilfiker
Pre-clinical and clinical data have unequivocally demonstrated the usefulness of decellularized heart valve (HV) matrices implanted for HV replacement therapy. However, human donor valves applicable for decellularization are in short supply, which prompts the search for suitable alternatives, such as porcine grafts. Since decellularization might be insufficient to remove all xenoantigens, we analysed the interaction of human preformed antibodies with decellularized porcine HV in vitro to assess potential immune reactions upon implantation...
July 2016: Basic Research in Cardiology
Kamal Hany Hussein, Kyung-Mee Park, Kyung-Sun Kang, Heung-Myong Woo
UNLABELLED: Whole organ decellularization is a cell removal process that creates a natural extracellular matrix for use in transplantation. A lack of an intact endothelial layer in the vascular network of decellularized organs results in blood clotting even with anti-coagulation treatment. Furthermore, shear stress caused by blood flow may affect reseeded parenchymal cells. We hypothesized that a heparin-gelatin mixture (HG) can act as an antithrombotic coating reagent and induce attachment and migration of endothelial cells (ECs) on vascular wall surfaces within decellularized livers, with subsequent parenchymal cell function enhancement...
July 1, 2016: Acta Biomaterialia
Sarah E Gilpin, Jonathan M Charest, Xi Ren, Harald C Ott
Whole lung extracellular matrix scaffolds can be created by perfusion of cadaveric organs with decellularizing detergents, providing a platform for organ regeneration. Lung epithelial engineering must address both the proximal airway cells that function to metabolize toxins and aid mucociliary clearance and the distal pneumocytes that facilitate gas exchange. Engineered pulmonary vasculature must support in vivo blood perfusion with low resistance and intact barrier function and be antithrombotic. Repopulating the native lung matrix with sufficient cell numbers in appropriate anatomic locations is required to enable organ function...
May 2016: Thoracic Surgery Clinics
Nima Momtahan, Tayyebeh Panahi, Nafiseh Poornejad, Michael G Stewart, Brady R Vance, Jeremy A Struk, Arthur A Castleton, Beverly L Roeder, Sivaprasad Sukavaneshvar, Alonzo D Cook
Developing patient-specific transplantable organs is a promising response to the increasing need of more effective therapies for patients with organ failure. Advances in tissue engineering strategies have demonstrated favorable results, including the use of decellularized hearts as scaffolds for cardiac engineering; however, there is a need to establish methods to characterize the cytotoxicity and blood compatibility of cardiac extracellular matrix (cECM) scaffolds created by decellularization. In this study, porcine hearts were decellularized in an automated perfusion apparatus utilizing sodium dodecyl sulfate (SDS) detergent...
May 2016: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Jessica L Ungerleider, Todd D Johnson, Melissa J Hernandez, Dean I Elhag, Rebecca L Braden, Monika Dzieciatkowska, Kent G Osborn, Kirk C Hansen, Ehtisham Mahmud, Karen L Christman
OBJECTIVE: This study aimed to examine acellular extracellular matrix based hydrogels as potential therapies for treating peripheral artery disease (PAD). We tested the efficacy of using a tissue specific injectable hydrogel, derived from decellularized porcine skeletal muscle (SKM), compared to a new human umbilical cord derived matrix (hUC) hydrogel, which could have greater potential for tissue regeneration because of its young tissue source age. BACKGROUND: The prevalence of PAD is increasing and can lead to critical limb ischemia (CLI) with potential limb amputation...
January 2016: JACC. Basic to Translational Science
Micha Sam Brickman Raredon, Kevin A Rocco, Ciprian P Gheorghe, Amogh Sivarapatna, Mahboobe Ghaedi, Jenna L Balestrini, Thomas L Raredon, Elizabeth A Calle, Laura E Niklason
Decellularized organs are now established as promising scaffolds for whole-organ regeneration. For this work to reach therapeutic practice, techniques and apparatus are necessary for doing human-scale clinically applicable organ cultures. We have designed and constructed a bioreactor system capable of accommodating whole human or porcine lungs, and we describe in this study relevant technical details, means of assembly and operation, and validation. The reactor has an artificial diaphragm that mimics the conditions found in the chest cavity in vivo, driving hydraulically regulated negative pressure ventilation and custom-built pulsatile perfusion apparatus capable of driving pressure-regulated or volume-regulated vascular flow...
2016: BioResearch Open Access
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