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Broncho alveolar lavage

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https://www.readbyqxmd.com/read/28724562/the-role-of-macrophages-in-interstitial-lung-diseases-number-3-in-the-series-pathology-for-the-clinician-edited-by-peter-dorfm%C3%A3-ller-and-alberto-cavazza
#1
Giulio Rossi, Alberto Cavazza, Paolo Spagnolo, Salvatore Bellafiore, Elisabetta Kuhn, Pierpaolo Carassai, Laura Caramanico, Gloria Montanari, Gaia Cappiello, Alessandro Andreani, Francesca Bono, Nazarena Nannini
The finding of collections of macrophages/histiocytes in lung biopsy and bronchoalveolar lavage is relatively common in routine practice. This morphological feature in itself is pathological, but the exact clinical significance and underlying disease should be evaluated together with clinical data, functional respiratory and laboratory tests and imaging studies.Morphological characteristics of macrophages and their distribution along the different pulmonary structures should be examined carefully by pathologists...
September 30, 2017: European Respiratory Review: An Official Journal of the European Respiratory Society
https://www.readbyqxmd.com/read/28716387/respiratory-virus-prior-to-hematopoietic-cell-transplantation-is-associated-with-allo-immune-mediated-lung-syndromes
#2
Birgitta Versluys, Marc Bierings, Jean Luc Murk, Tom Wolfs, Caroline Lindemans, Kors Vd Ent, Jaap Jan Boelens
BACKGROUND: Allo-immune mediated lung syndromes (allo-LS) are life-threatening complications after hematopoietic cell transplantation (HCT). Respiratory virus (RV) has been suggested to play a role in the pathogenesis. OBJECTIVE: We studied the relation between RV-DNA/RNA detection in upper/lower airway before HCT and the occurrence of allo-LS. METHODS: We retrospectively analyzed all HCT recipients between 2004 and 2014, in whom Real-Time PCR for RV was done from Nasal-Pharyngeal-Aspirates (NPA) and Broncho-Alveolar- Lavage (BAL) before HCT...
July 14, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28715731/metabolomics-analysis-reveals-that-benzo-a-pyrene-a-component-of-pm2-5-promotes-pulmonary-injury-by-modifying-lipid-metabolism-in-a-phospholipase-a2-dependent-manner-in-vivo-and-in-vitro
#3
Song-Yang Zhang, Danqing Shao, Huiying Liu, Juan Feng, Baihuan Feng, Xiaoming Song, Qian Zhao, Ming Chu, Changtao Jiang, Wei Huang, Xian Wang
Particulate matter with an aerodynamic diameter less than 2.5μM (PM2.5) is one of the major environmental pollutants in China. In this study, we carried out a metabolomics profile study on PM2.5-induced inflammation. PM2.5 from Beijing, China, was collected and given to rats through intra-tracheal instillation in vivo. Acute pulmonary injury were observed by pulmonary function assessment and H.E. staining. The lipid metabolic profile was also altered with increased phospholipid and sphingolipid metabolites in broncho-alveolar lavage fluid (BALF) after PM2...
July 4, 2017: Redox Biology
https://www.readbyqxmd.com/read/28711528/comparison-of-five-methods-for-isolation-of-dna-from-mycoplasma-cynos
#4
Aslı Sakmanoglu, Zafer Sayin, Uckun Sait Ucan, Yasemin Pinarkara, Ali Uslu, Osman Erganis
Extraction of DNA from Mycoplasma cultured on agar medium is difficult because the plasticity of these microorganisms enables agar penetration. This eventually causes cell loss during harvesting of colonies from the agar surface. Here, we used the GenElute™ gel extraction kit, which is usually used to purify polymerase chain reaction products, for extracting DNA from Mycoplasma. We compared the DNA extraction efficiency of the GenElute™ gel extraction kit from Mycoplasma cynos cultured in agar medium with four other DNA extraction methods...
July 12, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28683724/first-report-of-cavitary-pneumonia-due-to-community-acquired-acinetobacter-pittii-study-of-virulence-and-overview-of-pathogenesis-and-treatment
#5
Romaric Larcher, Alix Pantel, Erik Arnaud, Albert Sotto, Jean-Philippe Lavigne
BACKGROUND: Acinetobacter pittii is a nosocomial pathogen rarely involved in community-acquired infections. We report for the first time that A. pittii can be responsible for cavitary community-acquired pneumonia and study its virulence, and discuss its pathogenesis and treatment options. CASE PRESENTATION: A 45-year-old woman with a history of smoking and systemic lupus was admitted to Nimes University Hospital (France) with coughing and sputum lasting for three weeks...
July 6, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28586022/small-protein-a-and-phospholipase-d-immunization-serves-a-protective-role-in-a-mouse-pneumonia-model-of-acinetobacter%C3%A2-baumannii-infection
#6
Haitao Li, Hongyi Tan, Yongbin Hu, Pinhua Pan, Xiaoli Su, Chengpin Hu
Acinetobacter baumannii is an important pathogen that primarily causes hospital-acquired pneumonia. The present study sought to investigate whether small protein A (SmpA) and phospholipase D (PLD) are potential candidates for protective immunity against infection with A. baumannii. Mice immunized with the fusion proteins histidine (His)‑SmpA and His‑PLD exhibited a specific immunoglobulin G response. In a pneumonia model, active and passive immunization against SmpA and PLD protected mice from A. baumannii infection...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28575809/lutein-mitigates-cyclophosphamide-induced-lung-and-liver-injury-via-nf-%C3%AE%C2%BAb-mapk-dependent-mechanism
#7
Amal A El-Kholy, Mohamed A Elkablawy, Dina S El-Agamy
This study targeted to test the potential protective role of lutein against lung and liver damage associated with cyclophosphamide (CP) administration. Lutein was given orally for 5days at two different doses both before and after CP injection. Results have shown that CP administration caused marked pulmonary and hepatic injurious effects in mice. Lung damage was evident through increased lung wet/dry ratio, elevated inflammatory cells infiltration into the pulmonary tissues, increased total protein content and lactate dehydrogenase (LDH) activity in the broncho-alveolar lavage fluid...
August 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28562365/role-of-the-inflammasome-in-chronic-obstructive-pulmonary-disease-copd
#8
Chiara Colarusso, Michela Terlizzi, Antonio Molino, Aldo Pinto, Rosalinda Sorrentino
Inflammation is central to the development of chronic obstructive pulmonary disease (COPD), a pulmonary disorder characterized by chronic bronchitis, chronic airway obstruction, emphysema, associated to progressive and irreversible decline of lung function. Emerging genetic and pharmacological evidence suggests that IL-1-like cytokines are highly detected in the sputum and broncho-alveolar lavage (BAL) of COPD patients, implying the involvement of the multiprotein complex inflammasome. So far, scientific evidence has focused on nucleotide-binding oligomerization domain-like receptors protein 3 (NLRP3) inflammasome, a specialized inflammatory signaling platform that governs the maturation and secretion of IL-1-like cytokines through the regulation of caspase-1-dependent proteolytic processing...
May 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/28523329/respiratory-bronchiolitis-associated-interstitial-lung-disease-an-unexpected-form-of-idiopathic-interstitial-pneumonia-in-a-young-male
#9
Claudia Lucia Toma, Elena Danteş, Diana Gabriela Leonte, Ariadna Petronela Fildan
Cigarette smoking is the most frequently encountered risk factor for chronic obstructive pulmonary disease and lung cancer. The latest American Thoracic Society÷European Respiratory Society classification of idiopathic interstitial pneumonia includes two entities related to smoking habits: respiratory bronchiolitis-associated interstitial lung disease and desquamative interstitial pneumonia. The new approach to diagnosis is to combine pathological pattern with clinical and radiological data. Lung biopsy is no longer considered the "gold standard" for diagnosis, but as a part of the diagnosis, which shall be set only after the pulmonologist, radiologist and pathologist reviewed all clinical, imaging and pathological aspects...
2017: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/28504568/lactobacillus-rhamnosus-crl1505-nasal-administration-improves-recovery-of-t-cell-mediated-immunity-against-pneumococcal-infection-in-malnourished-mice
#10
N Barbieri, M Herrera, S Salva, J Villena, S Alvarez
Immunobiotic lactic acid bacteria have become an interesting alternative for the prevention of respiratory infections. Previously, we demonstrated that the nasal administration of Lactobacillus rhamnosus CRL1505, during repletion of malnourished mice, resulted in diminished susceptibility to the challenge with the respiratory pathogen Streptococcus pneumoniae. Considering the known alterations induced by malnutrition on T lymphocytes and the importance of this cell population on the protection against respiratory pathogens, we aimed to study the effect of L...
May 15, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28498407/respiratory-syncytial-virus-infection-accelerates-lung-fibrosis-through-the-unfolded-protein-response-in-a-bleomycin-induced-pulmonary-fibrosis-animal-model
#11
Lina Wang, Wei Cheng, Zhimin Zhang
Emerging evidence has demonstrated that endoplasmic reticulum stress (ER) is involved in the pathogenesis of idiopathic pulmonary fibrosis, however, the underlying mechanism remains unclear. Viral infection often triggers a hyperinflammatory response by an expansion of the ER. The present study was designed to observe the role of respiratory syncytial virus infection (RSV)‑induced ER stress on lung fibrosis. In order to determine the role of ER stress on the onset and progression of pulmonary fibrosis, mice received an intratracheal combined injection of RSV and bleomycin on day 0...
July 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28484089/xenogeneic-human-umbilical-cord-derived-mesenchymal-stem-cells-reduce-mortality-in-rats-with-acute-respiratory-distress-syndrome-complicated-by-sepsis
#12
Fan-Yen Lee, Kuan-Hung Chen, Christopher Glenn Wallace, Pei-Hsun Sung, Jiunn-Jye Sheu, Sheng-Ying Chung, Yung-Lung Chen, Hung-I Lu, Sheung-Fat Ko, Cheuk-Kwan Sun, Hsin-Ju Chiang, Hsueh-Wen Chang, Mel S Lee, Hon-Kan Yip
This study tested the hypothesis that xenogeneic human umbilical cord-derived mesenchymal stem cell (HUCDMSC) therapy would improve survival rates in rats with acute respiratory distress-syndrome (ARDS, induction by 48 h inhalation of 100% oxygen) and sepsis-syndrome (SS, induction by cecal-ligation and puncture) (ARDS-SS). Adult-male Sprague-Dawley rats were categorized into group 1 (sham-controls), group 2 (ARDS-SS), group 3 [ARDS-SS+HUCDMSC (1.2 ×106 cells administered 1 h after SS-induction)], and group 4 [ARDS-SS+HUCDMSC (1...
July 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28476111/single-use-and-conventional-bronchoscopes-for-broncho-alveolar-lavage-bal-in-research-a-comparative-study-nct-02515591
#13
Seher Raza Zaidi, Andrea M Collins, Elena Mitsi, Jesús Reiné, Kayleigh Davies, Angela D Wright, Jessica Owugha, Richard Fitzgerald, Amitava Ganguli, Stephen B Gordon, Daniela Mulari Ferreira, Jamie Rylance
BACKGROUND: Broncho alveolar lavage (BAL) is widely used for investigative research to study innate, cellular and humoral immune responses, and in early phase drug trials. Conventional (multiple use) flexible bronchoscopes have time and monetary costs associated with cleaning, and carries a small risk of cross infection. Single use bronchoscopes may provide an alternative, but have not been evaluated in this context. METHODS: Healthy volunteers underwent bronchoscopy at a day-case clinical research unit using the Ambu® aScope(TM) single-use flexible intubation bronchoscope...
May 5, 2017: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/28471870/the-effect-of-immunosuppression-on-airway-integrity
#14
Hannelore Bellon, Elly Vandermeulen, Stijn E Verleden, Tobias Heigl, Hanne Vriens, Elise Lammertyn, Lieve Verlinden, Thomas Vanhove, Mieke Verstuyf, Peter Hoet, Robin Vos, Geert M Verleden, Bart M Vanaudenaerde
BACKGROUND: Insults to the airway epithelium play a key role in constrictive bronchiolitis following lung transplantation (LTx), the typical hallmark of chronic rejection. Our hypothesis is that immunosuppressives might affect airway integrity. METHODS: A biculture of lung bronchial epithelial cells (16HBE) and lung microvascular endothelial cells (HLMVEC) was exposed to immunosuppressives (serum through levels) for 24h or 4 days. Cytotoxicity, trans-epithelial electrical resistance (TEER) and permeability was measured after exposure to monotherapies and combination therapies...
May 4, 2017: Transplantation
https://www.readbyqxmd.com/read/28415591/the-purinergic-receptor-subtype-p2y2-mediates-chemotaxis-of-neutrophils-and-fibroblasts-in-fibrotic-lung-disease
#15
Tobias Müller, Susanne Fay, Rodolfo Paula Vieira, Harry Karmouty-Quintana, Sanja Cicko, Korcan Ayata, Gernot Zissel, Torsten Goldmann, Giuseppe Lungarella, Davide Ferrari, Francesco Di Virgilio, Bernard Robaye, Jean-Marie Boeynaems, Michael R Blackburn, Marco Idzko
Idiopathic pulmonary fibrosis (IPF) is a devastating disease with few available treatment options. Recently, the involvement of purinergic receptor subtypes in the pathogenesis of different lung diseases has been demonstrated. Here we investigated the role of the purinergic receptor subtype P2Y2 in the context of fibrotic lung diseases.The concentration of different nucleotides was measured in the broncho-alveolar lavage (BAL) fluid derived from IPF patients and animals with bleomycin-induced pulmonary fibrosis...
May 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28412654/nigella-sativa-a-traditional-tunisian-herbal-medicine-attenuates-bleomycin-induced-pulmonary-fibrosis-in-a-rat-model
#16
Anouar Abidi, Alexandre Robbe, Nadia Kourda, Saloua Ben Khamsa, Alexandre Legrand
The present study investigated the effects of Nigella sativa oil (NSO) on bleomycin (BLM)-induced lung fibrosis in rats. The rat model of pulmonary fibrosis (PF) was established by intratracheal instillation of BLM, and the effect of 1ml/kg oral NSO treatment once daily observed. The effect of NSO was studied over a period of 50daysusing (1)H RMN analysis on the urine and broncho alveolar lavage fluid (Balf) of the rats. Histopathological (inflammation and fibrosis) and immunohistochemical (TGF-β1 density) changes were evaluated...
June 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28400838/hemohim-a-herbal-preparation-alleviates-airway-inflammation-caused-by-cigarette-smoke-and-lipopolysaccharide
#17
Na-Rae Shin, Sung-Ho Kim, Je-Won Ko, Sung-Hyeuk Park, In-Chul Lee, Jung-Min Ryu, Jong-Choon Kim, In-Sik Shin
HemoHIM, herbal preparation has designed for immune system recovery. We investigated the anti-inflammatory effect of HemoHIM on cigarette smoke (CS) and lipopolysaccharide (LPS) induced chronic obstructive pulmonary disease (COPD) mouse model. To induce COPD, C57BL/6 mice were exposed to CS for 1 h per day (eight cigarettes per day) for 4 weeks and intranasally received LPS on day 26. HemoHIM was administrated to mice at a dose of 50 or 100 mg/kg 1h before CS exposure. HemoHIM reduced the inflammatory cell count and levels of tumor necrosis factor receptor (TNF)-α, interleukin (IL)-6 and IL-1β in the broncho-alveolar lavage fluid (BALF) induced by CS+LPS exposure...
March 2017: Laboratory Animal Research
https://www.readbyqxmd.com/read/28380065/potential-use-of-local-and-systemic-humoral-immune-response-parameters-to-forecast-mycoplasma-hyopneumoniae-associated-lung-lesions
#18
Beatriz Garcia-Morante, Joaquim Segalés, Lorenzo Fraile, Gemma Llardén, Teresa Coll, Marina Sibila
Immunopathological events are key for the development of enzootic pneumonia (EP), which is macroscopically observed as cranioventral pulmonary consolidation (CVPC). This study aimed to investigate the putative association between the humoral immune response against Mycoplasma hyopneumoniae (M. hyopneumoniae) and prevalence and extension of CVPC in 1) experimentally infected pigs, 2) slaughtered pigs and 3) sequentially necropsied pigs in a longitudinal study. CVPC was scored by means of the European Pharmacopoeia recommended methodology...
2017: PloS One
https://www.readbyqxmd.com/read/28370571/investigation-of-inflammatory-and-allergic-responses-to-common-mold-species-results-from-in-vitro-experiments-from-a-mouse-model-of-asthma-and-from-a-group-of-asthmatic-patients
#19
Muriel Vincent, Pauline Percier, Sofie De Prins, Kris Huygen, Georges Potemberg, Eric Muraille, Marta Romano, Olivier Michel, Olivier Denis
Most studies on molds focus on A. alternata and A. fumigatus. Here we report on inflammatory and allergenic properties of more typical indoor species A. versicolor, P. chrysogenum, C. cladosporioïdes and C. sphaerospermum that were compared to A. alternata and A. fumigatus. In a mouse model, after intranasal instillation, A. alternaria, A. versicolor and C. sphaerospermum induced the early recruitment of neutrophils and the strong expression of inflammatory markers in the broncho-alveolar lavages fluids. A...
April 2, 2017: Indoor Air
https://www.readbyqxmd.com/read/28348765/progressive-disseminated-histoplasmosis-mimicking-a-flare-of-systemic-lupus-erythematosus-a-european-case-report
#20
M L M van Doorn-Schepens, E J Peters, R M van Vugt, J I van der Spoel, K van Dijk
INTRODUCTION: Diagnosing progressive disseminated histoplasmosis (PDH) in patients with systemic lupus erythematosus (SLE) is diagnostically challenging. Since PDH is lethal when untreated, awareness of this infection in patients with SLE is of utmost importance. To the best of our knowledge, this is the first description of a case of PDH in a patient with SLE in Europe. CASE PRESENTATION: A 56-year-old woman of Surinamese descent with a history of SLE, presented with fever and polyarthritis...
August 2016: JMM Case Reports
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