keyword
MENU ▼
Read by QxMD icon Read
search

Broncho alveolar lavage

keyword
https://www.readbyqxmd.com/read/29449421/increased-t-helper-17-1-cells-in-sarcoidosis-mediastinal-lymph-nodes
#1
Caroline E Broos, Laura L Koth, Menno van Nimwegen, Johannes C C M In't Veen, Sandra M J Paulissen, Jan Piet van Hamburg, Jouke T Annema, Roxane Heller-Baan, Alex Kleinjan, Henk C Hoogsteden, Marlies S Wijsenbeek, Rudi W Hendriks, Bernt van den Blink, Mirjam Kool
Objective: The lung-draining mediastinal lymph nodes (MLN) are currently widely used to diagnose sarcoidosis. We previously reported that T helper 17.1 (Th17.1) cells are responsible for the exaggerated IFN-γ production in sarcoidosis lungs. In this study, we aimed to investigate (i) whether Th17.1 cells are also increased in the MLN of sarcoidosis patients, and (ii) whether frequencies of the Th17.1 cells at diagnosis may correlate with disease progression. Methods: MLN cells from treatment-naive pulmonary sarcoidosis patients (n=17) and healthy controls (n=22) and peripheral blood (PB) mononuclear cells (n=34) and broncho-alveolar lavage fluid (BALF) (n=36) from sarcoidosis patients were examined for CD4 + T cell subset proportions, using flow cytometry...
February 15, 2018: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/29405435/immediate-post-operative-broncho-alveolar-lavage-il-6-and-il-8-are-associated-with-early-outcomes-after-lung-transplantation
#2
Stijn E Verleden, An Martens, Sofie Ordies, Arne P Neyrinck, Dirk E Van Raemdonck, Geert M Verleden, Bart M Vanaudenaerde, Robin Vos
INTRODUCTION: Previous studies demonstrated that increased cytokine and chemokine levels, either shortly before or after lung transplantation, were associated with post-transplant outcome. However, small patient cohorts were mostly used, focusing on one molecule and one outcome. In a large single-center cohort, we investigated the predictive value of immediate post-operative bronchoalveolar lavage (BAL) expression of IL-6 and IL-8 on multiple key outcomes, including PGD, CLAD, graft survival, as well as several secondary outcomes...
February 6, 2018: Clinical Transplantation
https://www.readbyqxmd.com/read/29395568/-pulmonary-granuloma-in-an-immunodepressed-patient
#3
F Gadby, L Quaesaet, C Eveilleau, A Payet-Charneau, F Couturaud, C Tromeur
INTRODUCTION: Pulmonary alveolar echinococcosis is a rare but potentially severe condition. CASE REPORT: We report the case of a 50-year-old woman suffering from pulmonary alveolar echinococcosis who had had a renal transplant for polycystic liver and kidney disease. A lung opacity was identified radiologically in May 2013. Both broncho-alveolar lavage and bronchial biopsy were uninformative. In January 2014, a follow up CT-scan showed the opacity to be enlarging...
January 27, 2018: Revue des Maladies Respiratoires
https://www.readbyqxmd.com/read/29379004/pneumomediastinum-and-pneumothorax-associated-with-herpes-simplex-virus-hsv-pneumonia
#4
Fermín López-Rivera, Xavier Colón Rivera, Hernán A González Monroig, Juan Garcia Puebla
BACKGROUND Pneumonia is one of the most common causes of death from infectious disease in the United States (US). Although most cases of community-acquired pneumonia (CAP) are secondary to bacterial infection, up to one-third of cases are secondary to viral infection, most commonly due to rhinovirus and influenza virus. Pneumonia due to herpes simplex virus (HSV) is rare, and there is limited knowledge of the pathogenesis and clinical complications. This report is of a fatal case of HSV pneumonia associated with bilateral pneumothorax and pneumomediastinum...
January 30, 2018: American Journal of Case Reports
https://www.readbyqxmd.com/read/29368146/irritant-induced-asthma-to-hypochlorite-in-mice-due-to-impairment-of-the-airway-barrier
#5
Sofie Van Den Broucke, Lore Pollaris, Greetje Vande Velde, Erik Verbeken, Benoit Nemery, Jeroen Vanoirbeek, Peter Hoet
Inhalation of commonly present irritants, such as chlorine and chlorine derivatives, can cause adverse respiratory effects, including irritant-induced asthma (IIA). We hypothesize that due to airway barrier impairment, exposure to hypochlorite (ClO-) can result in airway hypersensitivity. C57Bl/6 mice received an intra-peritoneal (i.p.) injection of the airway damaging agent naphthalene (NA, 200 mg/kg body weight) or vehicle (mineral oil, MO). In vivo micro-computed tomography (CT) images of the lungs were acquired before and at regular time points after the i...
January 24, 2018: Archives of Toxicology
https://www.readbyqxmd.com/read/29350567/pretreatment-of-ferulic-acid-attenuates-inflammation-and-oxidative-stress-in-a-rat-model-of-lipopolysaccharide-induced-acute-respiratory-distress-syndrome
#6
Sheng Zhang, Pengyu Wang, Pengxin Zhao, Dong Wang, Yanwei Zhang, Junhui Wang, Lixia Chen, Wenping Guo, Hui Gao, Yalou Jiao
Acute respiratory distress syndrome (ARDS) is a fatal clinical condition that can be caused by pulmonary and non-pulmonary diseases. Oxidative stress and inflammation play key roles in the development of ARDS. In this study, we investigated whether ferulic acid (FA), an anti-oxidant, was beneficial for prophylaxis of ARDS. We established an ARDS rat model using lipopolysaccharide (LPS) administration. Lung injury was assessed by lung wet/dry ratio and broncho-alveolar lavage fluid (BALF) analysis. Hematoxylin and eosin staining was performed to evaluate the histological changes of the lungs...
January 2018: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/29318623/subcutaneous-immunotherapy-with-purified-der-p1-and-2-suppresses-type-2-immunity-in-a-murine-asthma-model
#7
Laura Hesse, Nienke van Ieperen, Corine Habraken, Arjen H Petersen, Silvia Korn, Tim Smilda, Betty Goedewaagen, Marcel H Ruiters, Adrianus C van der Graaf, Martijn C Nawijn
Allergen-specific immunotherapy can induce long-term suppression of allergic symptoms, reduce medication use and prevent exacerbations of allergic rhinitis and asthma. Current treatment is based on crude allergen extracts, which contain immunostimulatory components such as β-glucans, chitins and endotoxin. Use of purified or recombinant allergens might therefore increase efficacy of treatment. Here, we test application of purified natural group 1 and 2 allergens from Dermatophagoides pteronyssinus (Der p) for subcutaneous immunotherapy (SCIT) treatment in a house dust mite (HDM) driven mouse model of allergic asthma...
January 10, 2018: Allergy
https://www.readbyqxmd.com/read/29311084/intrapulmonary-pharmacokinetics-of-relebactam-a-novel-%C3%AE-lactamase-inhibitor-dosed-in-combination-with-imipenem-cilastatin-in-healthy-subjects
#8
Matthew L Rizk, Elizabeth G Rhee, Patricia A Jumes, Mark H Gotfried, Tian Zhao, Eric Mangin, Sheng Bi, Cynthia M Chavez-Eng, Zufei Zhang, Joan R Butterton
This Phase I study assessed the intrapulmonary pharmacokinetic profiles of relebactam (MK-7655), a novel ß-lactamase inhibitor, and imipenem. Sixteen healthy subjects received 250 mg relebactam with 500 mg imipenem/cilastatin, given IV every 6 hours for 5 doses, and were randomized to bronchoscopy/broncho-alveolar lavage at 0.5, 1, 1.5, or 3 hours after the last dose (4 subjects per time point). Both drugs penetrated the epithelial lining fluid (ELF) to a similar degree, with profiles similar in shape to the corresponding plasma profiles and apparent terminal half-lives in plasma and ELF, respectively, of 1...
January 8, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29286940/sustained-lung-inflation-at-birth-for-preterm-infants-at-risk-of-respiratory-distress-syndrome-the-proper-pressure-and-duration
#9
N Abd El-Fattah, N Nasef, M F Al-Harrass, Mohammed Khashaba
BACKGROUND: Variations exist among the administered pressure and duration of sustained lung inflation (SLI) in the delivery room (DR). We aimed to evaluate the appropriate pressure and duration needed for SLI in preterm infants with respiratory distress syndrome. METHODS: We prospectively randomized 100 preterm (<32 weeks) infants to receive either conventional therapy of continuous positive airway pressure (CPAP) at 5 cm H2O, or four groups of CPAP plus a single maneuver of SLI at four regimens based on administered pressures and durations; P20D20 (Pressure of 20 cm H2O for a duration of 20 seconds), P20D10 (20 cm H2O for 10 seconds), P15D20 (15 cm H2O for 20 seconds), and P15D10 (15 cm H2O for 10 seconds) using a T-piece ventilator...
2017: Journal of Neonatal-perinatal Medicine
https://www.readbyqxmd.com/read/29284419/serotype-distribution-and-antibiotic-resistance-of-streptococcus-pneumoniae-isolates-from-17-chinese-cities-from-2011-to-2016
#10
Chunjiang Zhao, Zongbo Li, Feifei Zhang, Xiaobing Zhang, Ping Ji, Ji Zeng, Bijie Hu, Zhidong Hu, Kang Liao, Hongli Sun, Rong Zhang, Bin Cao, Chao Zhuo, Wei Jia, Yaning Mei, Yunzhuo Chu, Xuesong Xu, Qing Yang, Yan Jin, Quan Fu, Xiuli Xu, Hongling Li, Lijun Wang, Yuxing Ni, Hongjie Liang, Hui Wang
BACKGROUND: Streptococcus pneumoniae, the leading pathogen of bacterial infections in infants and the elderly, is responsible for pneumococcal diseases with severe morbidity and mortality. Emergence of drug-resistant strains presented new challenges for treatment and prevention. Vaccination has proven to be an effective means of preventing pneumococcal infection worldwide. Detailed epidemiological information of antibiotic susceptibilities and serotype distribution will be of great help to the management of pneumococcal infections...
December 29, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29281663/circulating-and-broncho-alveolar-interleukin-6-in-relation-to-body-temperature-in-an-experimental-model-of-bovine-chlamydia-psittaci-infection
#11
Annette Prohl, Carola H Ostermann, Christoph D Rummel, Joachim Roth, Petra Reinhold
In rodent models of experimentally induced fever, the important role of interleukin-6 (IL-6) as a circulating endogenous pyrogen is well established. Studies employing larger animal species and real infections are scarce. Therefore, we assessed bioactive IL-6 in peripheral blood and in broncho-alveolar lavage fluid (BALF) of calves after intra-bronchial inoculation with vital Chlamydia psittaci (Cp), with inactivated Cp, or with BGM cells. Only calves inoculated with vital Cp developed fever (peak at 2-3 days after challenge) and significantly increased IL-6 activity...
2017: PloS One
https://www.readbyqxmd.com/read/29248586/improving-the-diagnosis-of-invasive-aspergillosis-by-the-detection-of-aspergillus-in-broncho-alveolar-lavage-fluid-comparison-of-non-culture-based-assays
#12
Hélène Guegan, Florence Robert-Gangneux, Christophe Camus, Sorya Belaz, Tony Marchand, Marion Baldeyrou, Jean-Pierre Gangneux
OBJECTIVES: This study aimed to evaluate new tools to diagnose invasive aspergillosis (IA) directly from broncho-alveolar lavage (BAL) samples. METHODS: All consecutive patients with suspected IA who underwent bronchoscopy with BAL were prospectively included. Mycological culture and ELISA detection of galactomannan (GM) were performed on BAL. Two in-house and two marketed PCR assays were used on BAL DNA extracts to detect Aspergillus species. Susceptibility testing was performed after culture; marketed PCR assays detected mutations in the CYP51A gene associated to resistance...
December 14, 2017: Journal of Infection
https://www.readbyqxmd.com/read/29231967/therapeutic-potential-of-porcine-bronchoalveolar-fluid-derived-mesenchymal-stromal-cells-in-a-pig-model-of-lps-induced-ali
#13
Mahesh Khatri, Levi Arthur Richardson
In this study, we isolated mesenchymal stromal (stem) cells (MSCs) from broncho-alveolar lavage fluid (BAL) of 2-6 week-old commercial pigs. BAL-MSCs displayed fibroblastic morphology and possessed self-renewal properties. Similar to bone-marrow MSCs, BAL-MSCs expressed mesenchymal markers and both cell types lacked the expression of hematopoetic markers. BAL-MSCs, when cultured in differentiation induction media, differentiated into adipocytes, osteocytes and chondrocytes. Next, we examined if BAL-MSCs have the ability to treat lipopolysaccharide (LPS)-induced acute lung injury (ALI) in a pig model...
December 12, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29231883/silver-nanoparticles-in-the-lung-toxic-effects-and-focal-accumulation-of-silver-in-remote-organs
#14
Martin Wiemann, Antje Vennemann, Franziska Blaske, Michael Sperling, Uwe Karst
The distribution of silver (Ag) into remote organs secondary to the application of Ag nanoparticles (Ag-NP) to the lung is still incompletely understood and was investigated in the rat with imaging methods. Dose-finding experiments were carried out with 50 nm- or 200 nm-sized polyvinyl pyrrolidine (PVP)-coated Ag-NP using alveolar macrophages in vitro and female rats, which received Ag-NP via intratracheal instillation. In the main study, we administered 37.5-300 µg per rat lung of the more toxic Ag50-PVP and assessed the broncho-alveolar lavage fluid (BALF) for inflammatory cells, total protein and fibronectin after three and 21 days...
December 12, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29223534/systemic-recovery-and-therapeutic-effects-of-transplanted-allogenic-and-xenogenic-mesenchymal-stromal-cells-in-a-rat-blunt-chest-trauma-model
#15
Elisa Maria Amann, Markus Thomas Rojewski, Sinja Rodi, Daniel Fürst, Jörg Fiedler, Annette Palmer, Sonja Braumüller, Markus Huber-Lang, Hubert Schrezenmeier, Rolf Erwin Brenner
BACKGROUND: Effective therapy of Acute Lung Injury (ALI) is still a major scientific and clinical problem. To define novel therapeutic strategies for sequelae of blunt chest trauma (TxT) like ALI/Acute Respiratory Distress Syndrome, we have investigated the immunomodulatory and regenerative effects of a single dose of ex vivo expanded human or rat mesenchymal stromal cells (hMSCs/rMSCs) with or without priming, immediately after the induction of TxT in Wistar rats. METHODS: We analyzed the histological score of lung injury, the cell count of the broncho alveolar lavage fluid (BAL), the change in local and systemic cytokine level and the recovery of the administered cells 24 h and 5 days post trauma...
December 6, 2017: Cytotherapy
https://www.readbyqxmd.com/read/29204871/bone-marrow-mesenchymal-stem-cells-combat-lipopolysaccharide-induced-sepsis-in-rats-via-amendment-of-p38-mapk-signaling-cascade
#16
Omnia S Zaki, Marwa M Safar, Afaf A Ain-Shoka, Laila A Rashed
Sepsis is a systemic inflammatory disorder which often occurs during extremely stressful conditions such as trauma, burn, shock, and infection. This study investigated the curative effects of bone marrow-derived mesenchymal stem cells (BM-MSCs) against hepatic, renal, and pulmonary responses caused by a single administration of lipopolysaccharide (LPS) (10 mg/kg, i.p) in rats. Treatment with BM-MSCs (5 × 105 in 0.1 ml PBS, i.p.) 3 h after LPS antagonized the LPS-induced increment of the liver enzymes (ALT, AST) and kidney functions (BUN, sCr)...
December 4, 2017: Inflammation
https://www.readbyqxmd.com/read/29174976/deposition-pattern-of-aerosolized-legionella-using-an-ex-vivo-human-porcine-respiratory-model
#17
Sophie Perinel, Valérie Forest, Mathilde Landraud, Jérémie Pourchez, Françoise Girardot, Serge Riffard, Magalie Stauffert, Jean-Michel Vergnon, Séverine Allegra
Legionella are bacteria responsible for severe lung pathologies. However how they enter and are deposited within the respiratory tract remains poorly documented. Data using animal testing led to the establishment of mathematical models allowing the estimation of aerosol dispersion risks. But direct extrapolation to humans is questionable and experimental models more physiologically representative of the inhalation route are welcome. The aim of this study was to develop a model as close as possible to the human anatomy and physiology allowing determining the deposition pattern of aerosolized Legionella while limiting in vivo experiments...
November 20, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/29172825/metal-load-assessment-in-patient-pulmonary-lavages-towards-a-comprehensive-mineralogical-analysis-including-the-nano-sized-fraction
#18
Valérie Forest, Jean-Michel Vergnon, Cyril Guibert, Dimitrios Bitounis, Lara Leclerc, Gwendoline Sarry, Jérémie Pourchez
Mineralogical analyses of clinical samples have been proved useful to identify causal relationship between exposure to airborne particles and pulmonary diseases. The most striking example is asbestosis where the assessment of asbestos bodies in patient lung samples has allowed defining values specific of pathologies. However, this type of analyses only considers the micro-sized fraction of the particles, neglecting the specific impact of nano-sized particles which have been otherwise shown to be reactive and able to induce biological effects...
November 25, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/29153063/-investigation-of-various-virulence-factors-of-klebsiella-pneumoniae-strains-isolated-from-nosocomial-infections
#19
Halit Kuş, Uğur Arslan, Hatice Türk Dağı, Duygu Fındık
Klebsiella pneumoniae is an opportunistic pathogen that commonly affects immunosuppressed patients and causes nosocomial infections. K.pneumoniae has a variety of virulence factors, especially capsule polysaccharide, hypermucoviscosity (HV), fimbriae, toxins and determinants for iron acquisition. The aim of this study was to detect the virulence factors in K.pneumoniae strains isolated from nosocomial infections in two years. Fifty three K.pneumoniae strains isolated from the samples of patients with nosocomial infections in the Medical Microbiology Laboratory of Selcuk University Faculty of Medicine Hospital between 2011 and 2013 were included in the study...
October 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29140132/mediation-of-the-single-walled-carbon-nanotubes-induced-pulmonary-fibrogenic-response-by-osteopontin-and-tgf-%C3%AE-1
#20
Timur O Khaliullin, Elena R Kisin, Ashley R Murray, Naveena Yanamala, Michael R Shurin, Dmitriy W Gutkin, Liliya M Fatkhutdinova, Valerian E Kagan, Anna A Shvedova
PURPOSE OF THE STUDY: A number of in vivo studies have shown that pulmonary exposure to carbon nanotubes (CNTs) may lead to an acute local inflammatory response, pulmonary fibrosis, and granulomatous lesions. Among the factors that play direct roles in initiation and progression of fibrotic processes are epithelial-mesenchymal transition and myofibroblasts recruitment/differentiation, both mediated by transforming growth factor-β1 (TGF-β1). Yet, other contributors to TGF-β1 associated signaling, such as osteopontin (OPN) has not been fully investigated...
October 2017: Experimental Lung Research
keyword
keyword
54984
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"