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https://www.readbyqxmd.com/read/29245144/the-myorobot-a-novel-automated-biomechatronics-system-to-assess-voltage-ca2-biosensors-and-active-passive-biomechanics-in-muscle-and-biomaterials
#1
M Haug, B Reischl, G Prölß, C Pollmann, T Buckert, C Keidel, S Schürmann, M Hock, S Rupitsch, M Heckel, T Pöschel, T Scheibel, C Haynl, L Kiriaev, S I Head, O Friedrich
We engineered an automated biomechatronics system, MyoRobot, for robust objective and versatile assessment of muscle or polymer materials (bio-)mechanics. It covers multiple levels of muscle biosensor assessment, e.g. membrane voltage or contractile apparatus Ca2+ ion responses (force resolution 1µN, 0-10mN for the given sensor; [Ca2+] range ~ 100nM-25µM). It replaces previously tedious manual protocols to obtain exhaustive information on active/passive biomechanical properties across various morphological tissue levels...
December 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29244891/the-effects-of-experimental-temporalis-muscle-pain-on-jaw-muscle-electromyographic-activity-during-jaw-movements-and-relationships-with-some-psychological-variables
#2
Tantry Maulina, Magda Amhamed, Terry Whittle, John Gal, Rahena Akhter, Greg M Murray
AIMS: To determine if the effects of experimental temporalis muscle pain on jaw muscle activity vary with the jaw task performed, jaw displacement magnitude, participant being studied, and with psychological measures. METHODS: Jaw movement was tracked, and electromyographic (EMG) activity was recorded from the masseter and anterior temporalis and digastric muscles in 14 asymptomatic participants during standardized opening/closing jaw movement, free chewing, and standardized chewing tasks...
December 15, 2017: Journal of Oral & Facial Pain and Headache
https://www.readbyqxmd.com/read/29243276/the-influence-of-shc-proteins-and-high-fat-diet-on-energy-metabolism-of-mice
#3
W A Baldassini, J J Ramsey, K Hagopian, D P D Lanna
The purpose of this study was to determine if Shc proteins influence the metabolic response to acute (7 days) feeding of a high-fat diet (HFD). To this end, whole animal energy expenditure (EE) and substrate oxidation were measured in the Shc knockout (ShcKO) and wild-type (WT) mice fed a control or HFD. The activities of enzymes of glycolysis, the citric acid cycle, electron transport chain (ETC), and β-oxidation were also investigated in liver and skeletal muscle of ShcKO and WT animals. The study showed that ShcKO increases (P < ...
December 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29242624/plant-derived-compounds-strigolactone-gr24-and-pinosylvin-activate-sirt1-and-enhance-glucose-uptake-in-rat-skeletal-muscle-cells
#4
Shalem Modi, Nagendra Yaluri, Tarja Kokkola, Markku Laakso
Insulin resistance is a characteristic finding in hyperglycaemia and type 2 diabetes. SIRT1 is a NAD+ dependent deacetylase that plays a central role in glucose homeostasis and energy metabolism. SIRT1 activators, including plant polyphenols such as resveratrol, improve insulin sensitivity in skeletal muscle tissue. We hypothesised that the novel plant-derived compounds, strigolactone and pinosylvin, beneficially enhance SIRT1 function, insulin signalling, glucose uptake, and mitochondrial biogenesis in skeletal muscle cells...
December 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29242277/endoplasmic-reticulum-chaperone-grp78-regulates-macrophage-function-and-insulin-resistance-in-diet-induced-obesity
#5
Jong Hun Kim, Eunjung Lee, Randall H Friedline, Sujin Suk, Dae Young Jung, Sezin Dagdeviren, Xiaodi Hu, Kunikazu Inashima, Hye Lim Noh, Jung Yeon Kwon, Aya Nambu, Jun R Huh, Myoung Sook Han, Roger J Davis, Amy S Lee, Ki Won Lee, Jason K Kim
Obesity-mediated inflammation is a major cause of insulin resistance, and macrophages play an important role in this process. The 78-kDa glucose-regulated protein (GRP78) is a major endoplasmic reticulum chaperone that modulates unfolded protein response (UPR), and mice with GRP78 heterozygosity were resistant to diet-induced obesity. Here, we show that mice with macrophage-selective ablation of GRP78 (Lyz-GRP78-/-) are protected from skeletal muscle insulin resistance without changes in obesity compared with wild-type mice after 9 wk of high-fat diet...
December 14, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29241172/neurophysiological-monitoring-and-awake-craniotomy-for-resection-of-intracranial-gliomas
#6
Taiichi Saito, Manabu Tamura, Mikhail F Chernov, Soko Ikuta, Yoshihiro Muragaki, Takashi Maruyama
Aggressive resection of intracranial gliomas has a positive impact on patients' prognosis, but is associated with a risk of neurological complications. For preservation of brain functions and avoidance of major postoperative morbidity various methods of intraoperative neurophysiological monitoring have been introduced into clinical practice. At present, somatosensory evoked potentials (SSEP), motor evoked potentials (MEP), visual evoked potentials (VEP), brainstem auditory evoked potentials (BAEP), and electrocorticography (ECoG) are used routinely during neurosurgical procedures...
2018: Progress in Neurological Surgery
https://www.readbyqxmd.com/read/29240948/early-cyclical-neuromuscular-electrical-stimulation-improves-strength-and-trophism-by-akt-pathway-signaling-in-partially-paralyzed-biceps-muscle-after-spinal-cord-injury-in-rats
#7
Gabriel R de Freitas, Caroline C do Espírito Santo, Nicolas A M M de Machado-Pereira, Franciane Bobinski, Adair R S Dos Santos, Jocemar Ilha
Background: Electrical stimulation is often used to treat weakness in people with spinal cord injury (SCI), however its efficacy to increase strength and trophism are weak and the mechanisms underlying the therapeutic benefits are unknown. Objective: The purpose of the study was to analyze the effects of neuromuscular electrical stimulation (NMES) on muscle function, trophism and the Akt pathway signaling involved in muscular plasticity after incomplete-SCI in rats...
December 12, 2017: Physical Therapy
https://www.readbyqxmd.com/read/29239825/palpebral-portion-of-the-orbicularis-oculi-muscle-to-repetitive-nerve-stimulation-testing-a-potential-assessment-indicator-in-patients-with-generalized-myasthenia-gravis
#8
Chong Yan, Jie Song, Song Pang, Fangfang Yi, Jianying Xi, Lei Zhou, Ding Ding, Weifeng Wang, Kai Qiao, Chongbo Zhao
Repetitive nerve stimulation (RNS) is a valuable diagnostic method for myasthenia gravis (MG). However, its association with clinical severity was scarcely studied. We reviewed medical records and retrospectively enrolled 121 generalized MG patients. Sensitivity of different muscles to RNS and clinical scoring systems was evaluated. RNS testing revealed facial muscles have the highest positive rate, followed by proximal muscles and distal muscles, with the palpebral portion of the orbicularis oculi muscle most sensitive...
November 24, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/29238372/transplantation-of-rat-mesenchymal-stem-cells-overexpressing-hypoxia-inducible-factor-2%C3%AE-improves-blood-perfusion-and-arteriogenesis-in-a-rat-hindlimb-ischemia-model
#9
Weifeng Lu, Xiaoli Chen, Yi Si, Shichai Hong, Zhengyu Shi, Weiguo Fu
Mesenchymal stem cells (MSCs) have been increasingly tested in cell-based therapy to treat numerous diseases. Genetic modification to improve MSC behavior may enhance posttransplantation outcome. This study aims to test the potential therapeutic benefits of rat bone marrow MSCs overexpressing hypoxia-inducible factor 2α (rMSCsHIF-2α ) in a rat hindlimb ischemia model. PBS, rMSCs, or rMSCsHIF-2α were injected into rat ischemic hindlimb. Compared with the injection of PBS or rMSCs, transplantation of rMSCsHIF-2α significantly improved blood perfusion, increased the number of vessel branches in the muscle of the ischemic hindlimb, and improved the foot mobility of the ischemic hindlimb (all P < 0...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29238211/interdependency-between-mechanical-parameters-and-afferent-nerve-discharge-in-remodeled-diabetic-goto-kakizaki-rat-intestine
#10
Jingbo Zhao, Jian Yang, Donghua Liao, Hans Gregersen
Background: Gastrointestinal disorders are very common in diabetic patients, but the pathogenesis is still not well understood. Peripheral afferent nerves may be involved due to the complex regulation of gastrointestinal function by the enteric nervous system. Objective: We aimed to characterize the stimulus-response function of afferent fibers innervating the jejunum in the Goto-Kakizaki (GK) type 2 diabetic rat model. A key question is whether changes in afferent firing arise from remodeled tissue or from adaptive afferent processes...
2017: Clinical and Experimental Gastroenterology
https://www.readbyqxmd.com/read/29236890/phrenic-nerve-conduction-studies-normative-data-and-technical-aspects
#11
Analucia Abreu Maranhão, Sonia Regina da Silva Carvalho, Marcelo Ribeiro Caetano, Alexandre Hofke Alamy, Eduardo Mesquita Peixoto, Pedro Del Esporte Peçanha Filgueiras
OBJECTIVE: The aim of the present study was to define normative data of phrenic nerve conduction parameters of a healthy population. METHODS: Phrenic nerve conduction studies were performed in 27 healthy volunteers. RESULTS: The normative limits for expiratory phrenic nerve compound muscle action potential were: amplitude (0.47 mv - 0.83 mv), latency (5.74 ms - 7.10 ms), area (6.20 ms/mv - 7.20 ms/mv) and duration (18.30 ms - 20.96 ms). Inspiratory normative limits were: amplitude (0...
December 2017: Arquivos de Neuro-psiquiatria
https://www.readbyqxmd.com/read/29236301/synergistic-effect-of-carnosine-on-browning-of-adipose-tissue-in-exercised-obese-rats-a-focus-on-circulating-irisin-levels
#12
Mona F Schaalan, Basma K Ramadan, Azza H Abd Elwahab
BACKGROUND: The recent appreciation of the energy burning capacity of brown adipose tissue turns it to an attractive target as anti-obesity therapy. OBJECTIVE: to evaluate the effect of L-carnosine on browning of white adipose tissue in exercised obese rats. METHODS: Sixty adult male Wistar albino rats between 7-8 weeks-old weighing 130-150 g were allocated into six groups;(i) normal control rats fed normal diet; (ii) high fat diet (HFD)-induced obese rats, (iii) normal control rats fed normal diet and injected with L-carnosine (250mg/kg), (iv) HFD-rats injected with L-carnosine (250mg/kg),(v): HFD-rats subjected to exercise training; (vi): HFD- rats subjected to exercise training and L-carnosine together...
December 13, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29235416/skeletal-muscle-cell-contraction-reduces-a-novel-myokine-chemokine-c-x-c-motif-ligand-10-cxcl10-potential-roles-in-exercise-regulated-angiogenesis
#13
Yuri Ishiuchi, Hitoshi Sato, Kazuki Tsujimura, Hideo Kawaguchi, Takashi Matsuwaki, Keitaro Yamanouchi, Masugi Nishihara, Taku Nedachi
Accumulating evidence indicates that skeletal muscle secrets proteins referred to as myokines and that exercise contributes to their regulation. In this study, we propose that chemokine (C-X-C motif) ligand 10 (CXCL10) functions as a novel myokine. Initially, we stimulated differentiated C2C12 myotubes with or without electrical pulse stimulation (EPS) to identify novel myokines. Cytokine array analysis revealed that CXCL10 secretion was significantly reduced by EPS, which was further confirmed by enzyme-linked immunosorbent assay and quantitative polymerase chain reaction analysis...
December 13, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29234703/lean-and-obese-zucker-rat-extensor-digitorum-longus-muscle-high-frequency-electrical-stimulation-hfes-data-regulation-of-p70s6kinase-associated-proteins
#14
Kevin M Rice, Anjaiah Katta, Nandini D P K Manne, Ravikumar Arvapalli, Gautam K Ginjupalli, Miaozong Wu, Shinichi Asano, Eric R Blough
Anaerobic exercise has been advocated as a prescribed treatment for the management of diabetes: however, alterations in exercise-induced signaling remain largely unexplored in the diabetic muscle. Here, we compare the basal and the in situ contraction-induced phosphorylation of the AKT, GSK3beta, mTor, p70s6K, Pten, and Shp2 in the lean and obese (fa/fa) Zucker rat Extensor Digitorum Longus (EDL) muscle following a single bout of contractile stimuli. This article represents data associated with prior publications from our lab (Katta et al...
February 2018: Data in Brief
https://www.readbyqxmd.com/read/29234702/high-frequency-electrical-stimulation-hfes-data-lean-and-obese-zucker-rat-tibialis-anterior-muscle-regulation-of-glycogen-synthase-kinase-3-beta-gsk3b-associated-proteins
#15
Gautam K Ginjupalli, Kevin M Rice, Anjaiah Katta, Nandini D P K Manne, Ravikumar Arvapalli, Miaozong Wu, Shinichi Asano, Eric R Blough
Anaerobic exercise has been advocated as a prescribed treatment for the management of diabetes: however, alterations in exercise-induced signaling remain largely unexplored in the diabetic muscle. Here, we compare the basal and the in situ contraction-induced phosphorylation of the AMPK, GSK3beta, and Shp2 in the lean and obese (fa/fa) Zucker rat tibialis anterior (TA) muscle following a single bout of contractile stimuli. This article represents data associated with prior publications from our lab (Katta et al...
February 2018: Data in Brief
https://www.readbyqxmd.com/read/29234693/lean-and-obese-zucker-rat-extensor-digitorum-longus-muscle-high-frequency-electrical-stimulation-hfes-data-regulation-of-mapks-associated-proteins
#16
Kevin M Rice, Anjaiah Katta, Nandini D P K Manne, Ravikumar Arvapalli, Gautam K Ginjupalli, Miaozong Wu, Shinichi Asano, Eric R Blough
Anaerobic exercise has been advocated as a prescribed treatment for the management of diabetes: however, alterations in exercise-induced signaling remain largely unexplored in the diabetic muscle. Here, we compare the basal and the in situ contraction-induced phosphorylation of the mitogen-activated protein kinases (MAPKs) ERK 1/2, p38, and JNK in the lean and obese (fa/fa) Zucker rat extensor digitorum longus (EDL) muscle following a single bout of contractile stimuli. This article represents data associated with prior publications from our (Katta et al...
February 2018: Data in Brief
https://www.readbyqxmd.com/read/29234691/diabetic-zucker-rat-tibialis-anterior-muscle-high-frequency-electrical-stimulation-hfes-data-regulation-of-mapks-associated-proteins
#17
Gautam K Ginjupalli, Kevin M Rice, Anjaiah Katta, Nandini D P K Manne, Ravikumar Arvapalli, Miaozong Wu, Shinichi Asano, Eric R Blough
Anaerobic exercise has been advocated as a prescribed treatment for the management of diabetes: however, alterations in exercise-induced signaling remain largely unexplored in the diabetic muscle. Here, we compare the basal and the in situ contraction-induced phosphorylation of the mitogen-activated protein kinases (MAPKs) ERK 1/2, p38, and JNK in the lean and obese (fa/fa) Zucker rat tibialus anterior (TA) muscle following a single bout of contractile stimuli. This article represents data associated with prior publications from our lab (Katta et al...
February 2018: Data in Brief
https://www.readbyqxmd.com/read/29234690/the-dataset-describes-phenotypic-changes-induced-by-cholesterol-loading-in-smooth-muscle-cells-isolated-from-the-aortae-of-c57bl-6-mice
#18
Silvia Castiglioni, Matteo Monti, Giuditta Ainis Buscherini, Lorenzo Arnaboldi, Monica Canavesi, Alberto Corsini, Stefano Bellosta
The data presented in this article is related to the research article entitled "ABCA1 and HDL3 are Required to Modulate Smooth Muscle Cells Phenotypic Switch after Cholesterol Loading" (Castiglioni et al., 2017) [1]. This data describes the characterization of the phenotypic changes induced by cholesterol loading in smooth muscle cells (SMCs) isolated from the aortae of C57BL/6 mice. Upon cholesterol loading, there is a significant and concentration-dependent decrease in the expression of Acta2 and a parallel increase in Mac-2, and ATP binding cassette (ABC) transporters Abca1 and Abcg1...
February 2018: Data in Brief
https://www.readbyqxmd.com/read/29233823/large-cardiac-muscle-patches-engineered-from-human-induced-pluripotent-stem-cell-derived-cardiac-cells-improve-recovery-from-myocardial-infarction-in-swine
#19
Ling Gao, Zachery R Gregorich, Wuqiang Zhu, Saidulu Mattapally, Yasin Oduk, Xi Lou, Ramaswamy Kannappan, Anton V Borovjagin, Gregory P Walcott, Andrew E Pollard, Vladimir G Fast, Xinyang Hu, Steven G Lloyd, Ying Ge, Jianyi Zhang
Background -Here, we generated human cardiac muscle patches (hCMPs) of clinically relevant dimensions (4 cm × 2 cm × 1.25 mm) by suspending cardiomyocytes, smooth-muscle cells, and endothelial cells that had been differentiated from human induced-pluripotent stem cells (hiPSCs) in a fibrin scaffold and then culturing the construct on a dynamic (rocking) platform. Methods -In vitro assessments of hCMPs suggest maturation in response to dynamic culture stimulation. In vivo assessments were conducted in a porcine model of myocardial infarction (MI)...
December 12, 2017: Circulation
https://www.readbyqxmd.com/read/29233625/the-clinical-use-of-neuromuscular-electrical-stimulation-for-neuromuscular-rehabilitation-what-are-we-overlooking
#20
Nicola A Maffiuletti, Julien Gondin, Nicolas Place, Jennifer Stevens-Lapsley, Isabelle Vivodtzev, Marco A Minetto
The clinical success of neuromuscular electrical stimulation (NMES) for neuromuscular rehabilitation is greatly compromised by the poor consideration of different physiological and methodological issues that are not always obvious for the clinicians. Therefore, the aim of this narrative review is to re-examine some of these fundamental aspects of NMES using a tripartite model perspective. First, we contend that NMES does not actually bypass the central nervous system but results in a multitude of neurally-mediated responses that contribute substantially to force generation and may engender neural adaptations...
December 9, 2017: Archives of Physical Medicine and Rehabilitation
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