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Juliana Brum Moraes, Michael Maes, Chutima Roomruangwong, Kamila Landucci Bonifacio, Decio Sabbatini Barbosa, Heber Odebrecht Vargas, George Anderson, Marta Kubera, Andre F Carvalho, Sandra Odebrecht Vargas Nunes
Early life trauma (ELT) may increase the risk towards bipolar disorder (BD) and major depression (MDD), disorders associated with activated neuro-oxidative and neuro-nitrosative stress (O&NS) pathways. It has remained elusive whether ELTs are associated with O&NS and which ELTs are associated with distinct affective disorder phenotypes. This case-control study examined patients with BD (n = 68) and MDD (n = 37) and healthy controls (n = 66). The Child Trauma Questionnaire (CTQ) was used to assess specific ELT...
March 14, 2018: Metabolic Brain Disease
Taemoon Song, Ji Eun Park, Young Keun Chung
[Rh(COD)Cl]2/xantphos/Cs2CO3 efficiently catalyzes the reductive N-alkylation of aryl nitro compounds with alcohols by a borrowing-hydrogen strategy to afford the corresponding imine products in good to excellent yield. In the absence of xantphos, the [Rh(COD)Cl]2/Cs2CO3 catalytic system behaves as an effective catalyst for the dehydrogenative cou-pling of alcohols to esters, with nitrobenzene as hydrogen-acceptor. The reactivity of the rhodium catalytic system can be easily manipulated to selectively afford the imine or ester...
March 14, 2018: Journal of Organic Chemistry
L V Tat'yanenko, N A Sanina, O V Dobrokhotova, A I Kotelnikov, N S Goryachev, I I Pihteleva, G I Kozub, T A Kondrateva
The effect of iron nitrosyl complexes, NO donors, of a general formula [Fe2 (L)2 (NO)4 ] with functional sulfur-containing ligands (L-3-nitro-phenol-2-yl, 4-nitro-phenol-2-yl, or 1-methyl-tetrazol-5-yl) on the activity of sarcoplasmic reticulum Ca2+ -ATPase and cyclic guanosine monophosphate phosphodiesterase (cGMP PDE) was studied. The test complexes uncoupled the hydrolytic and transport functions of Ca2+ - ATPase, thus disturbing the balance of Ca2+ ions in cells, which may affect the formation of thrombi and adhesion of metastatic cells to the endothelium of capillaries...
January 2018: Doklady. Biochemistry and Biophysics
Kon-Ichi Arai, Tsutomu Yasukawa, Aki Kato, Ayae Kubota, Hideaki Usui, Noriaki Takase, Soichiro Kuwayama, Yuichiro Ogura
AIMS: Anti-vascular endothelial growth factor agents effectively treat age-related macular degeneration and myopic choroidal neovascularization (CNV). Tissue plasminogen activator (tPA), a fibrinolytic compound, is used as an adjuvant to displace submacular hemorrhage and to treat type 2 CNV. The purpose of this study was to investigate in in vitro and in vivo experiments the antiangiogenic impact of tPA itself. METHODS: The impact of tPA on the proliferation of human umbilical vein endothelial cells (HUVECs) was assessed by an XTT assay [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide]...
March 13, 2018: Ophthalmic Research
M Tavares-Silva, D Ferreira, S Cardoso, A R Raimundo, J Barbosa-Breda, A Leite-Moreira, A Rocha-Sousa
Urocortin 2 (UCN2) is a peptide related to corticotropin-releasing factor, capable of activating CRF-R2. Among its multisystemic effects, it has actions in all 3 muscle subtypes. This study's aim was to determine its potential role in two of the intrinsic eye muscle kinetics. Strips of iris sphincter (rabbit) and ciliary (bovine) muscles were dissected and mounted in isometric force-transducer systems filled with aerated-solutions. Contraction was elicited using carbachol (10(-6) M for iris sphincter, 10(-5) M for ciliary muscle), prior to all testing substances...
March 12, 2018: Physiological Research
Scott K Ferguson, Julie W Harral, David I Pak, Katherine M Redinius, Kurt R Stenmark, Dominik J Schaer, Paul W Buehler, David C Irwin
Free hemoglobin (Hb) associated with hemolysis extravasates into vascular tissue and depletes nitric oxide (NO), which leads to impaired vascular function and could impair skeletal muscle metabolic control during exercise. We tested the hypothesis that: 1) free Hb would extravasate into skeletal muscle tissue, reducing the contracting skeletal muscle O2 delivery/O2 utilization ratio (microvascular PO2 , PO2 mv) to a similar extent as that observed following NO synthase (NOS) blockade, and 2) that the Hb scavenging protein haptoglobin (Hp) would prevent Hb extravasation and inhibit these skeletal muscle tissue effects...
March 8, 2018: Nitric Oxide: Biology and Chemistry
Zhongjian Cheng, Xinggui Shen, Xiaohua Jiang, Huimin Shan, Maria Cimini, Pu Fang, Yong Ji, Joon Young Park, Konstantinos Drosatos, Xiaofeng Yang, Christopher G Kevil, Raj Kishore, Hong Wang
Insufficient hydrogen sulfide (H2 S) has been implicated in Type 2 diabetic mellitus (T2DM) and hyperhomocysteinemia (HHcy)-related cardiovascular complications. We investigated the role of H2 S in T2DM and HHcy-induced endothelial dysfunction in small mesenteric artery (SMA) of db/db mice fed a high methionine (HM) diet. HM diet (8 weeks) induced HHcy in both T2DM db/db mice and non-diabetic db/+ mice (total plasma Hcy: 48.4 and 31.3 µM, respectively), and aggravated the impaired endothelium-derived hyperpolarization factor (EDHF)-induced endothelium-dependent relaxation to acetylcholine (ACh), determined by the presence of eNOS inhibitor N(ω)-nitro-L-arginine methyl ester (L-NAME) and prostacyclin (PGI2 ) inhibitor indomethacin (INDO), in SMA from db/db mice but not that from db/+ mice...
February 14, 2018: Redox Biology
Archana Singh, Deepti Jain, Chetna Tyagi, Sujata Singh, Sumit Kumar, Indrakant K Singh
Plants are endowed with an innate immune system, which enables them to protect themselves from pest and pathogen. The participation of pathogenesis-related (PR) proteins is one of the most crucial events of inducible plant defense response. Herein, we report the characterization of CaHaPR-4, a Helicoverpa-inducible class II PR-4 protein from chickpea. Bioinformatic analysis of CaHaPR-4 protein indicated the presence of a signal peptide, barwin domain but it lacks the chitin-binding site/hevein domain. The recombinant CaHaPR-4 is bestowed with RNase and bivalent ion-dependent DNase activity...
March 7, 2018: International Journal of Biological Macromolecules
Cássio S Meira, José Maurício Dos Santos Filho, Caroline C Sousa, Pâmela S Anjos, Jéssica V Cerqueira, Humberto A Dias Neto, Rafael G da Silveira, Helena M Russo, Jean-Luc Wolfender, Emerson F Queiroz, Diogo R M Moreira, Milena B P Soares
4-(Nitrophenyl)hydrazone derivatives of N-acylhydrazone were synthesized and screened for suppress lymphocyte proliferation and nitrite inhibition in macrophages. Compared to an unsubstituted N-acylhydrazone, active compounds were identified within initial series when hydroxyl, chloride and nitro substituents were employed. Structure-activity relationship was further developed by varying the position of these substituents as well as attaching structurally-related substituents. Changing substituent position revealed a more promising compound series of anti-inflammatory agents...
February 25, 2018: Bioorganic & Medicinal Chemistry
Tinkara Mastnak, Aleksandra Lobnik, Gerhard J Mohr, Matejka Turel
The synthesis of two new azobenzene dyes, namely CR-528 and CR-555, and their spectral properties in ethanol solution are described. The recognition of sulfur-containing analytes (2-mercaptoethanol (2-ME), sodium hydrosulfide (NaHS)), and biogenic amines (spermine, spermidine, ethanolamine) bestowed significant spectral changes with color changes from pink/purple to pale yellow/orange-yellow. The nitro acceptor group in the dicyanovinyl reactive dye contributes to higher sensitivity and lower detected analyte concentrations...
March 8, 2018: Sensors
Francisco J Corpas
Peroxisome organelles have a versatile metabolism whose enzymatic content can be modulated by physiological and environment-dependent cellular conditions. They are characterized by a highly active nitro-oxidative metabolism and basic elements (H2 O2 and nitric oxide (NO)) with signaling properties. However, new elements have increased our understanding of the connections between peroxisomes and other cellular compartments. Furthermore, the presence of calcium (Ca2+ ) intensifies communication between different signaling molecules and the relationship of Ca2+ itself with NO and H2 O2 ...
March 8, 2018: Plant Signaling & Behavior
Piyush K Jain, Simon H Friedman
There is a need for methods to chemically incorporate photocleavable labels into synthetic and biologically sourced nucleic acids in a chemically defined and reversible manner. We have previously demonstrated that the light cleaved diazo-DMNPE group (diazo di-methoxy nitro phenyl ethyl) has a remarkable regiospecificity for modifying terminally phosphorylated siRNA. Building on this observation, we have identified conditions by which a new diazo-DMNPE reagent (diazo-DMNPE-azide or DDA) is able to singly modify any nucleic acid (RNA, DNA, single stranded, double stranded, 3' or 5' phosphate)...
March 7, 2018: Chembiochem: a European Journal of Chemical Biology
Carla Blum-Johnston, Richard B Thorpe, Chelsea Wee, Raechel Opsahl, Monica Romero, Samuel Murray, Alexander Brunelle, Quintin Blood, Rachael Wilson, Arlin B Blood, Lubo Zhang, Lawrence D Longo, William J Pearce, Sean M Wilson
Bradykinin-induced activation of the pulmonary endothelium triggers a rise in intracellular Ca2+ , which activates nitric oxide (NO)-dependent vasorelaxation. Chronic hypoxia is commonly associated with increased pulmonary vascular tone, which can cause pulmonary hypertension in responsive individuals. In the present study, we tested the hypothesis that long-term high altitude hypoxia (LTH) diminishes bradykinin-induced Ca2+ signals and inhibits eNOS, PGI2 and BKCa channels in sheep, which are moderately responsive to LTH, resulting in decreased pulmonary arterial vasorelaxation...
March 7, 2018: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Daniel Hirshberg, Daniel Sharon, Michal Afri, Ronit Lavi, Aryeh Frimer, Noa Metoki, Noam Eliaz, Won-Jin Kwak, Yang-Kook Sun, Doron Aurbach
We have developed a new effective in-operando methodology for investigating oxygen reduction reactions (ORR) and their mechanisms in non-aqueous solutions, based on UV-Vis spectroscopy in conjunction with various electrochemi-cal techniques. We can follow in-situ the formation and presence of superoxide moieties during ORR as a function of the solvent, cations, anions and additives in solution. Thus, using in-operando UV-Vis spectroscopy, we found evidence for the formation of superoxide radical anions during oxygen reduction in LiTFSI/diglyme electrolyte solutions...
March 7, 2018: ACS Applied Materials & Interfaces
Jun Du, Mahesh S Kodikara, Graeme J Moxey, Mahbod Morshedi, Adam Barlow, Cristóbal Quintana, Genmiao Wang, Robert Stranger, Chi Zhang, Marie P Cifuentes, Mark G Humphrey
1-Nitronaphthalenyl-4-alkynyl and 9-nitroanthracenyl-10-alkynyl complexes [M](C[triple bond, length as m-dash]C-4-C10 H6 -1-NO2 ) ([M] = trans-[RuCl(dppe)2 ] (6b), trans-[RuCl(dppm)2 ] (7b), Ru(PPh3 )2 (η5 -C5 H5 ) (8b), Ni(PPh3 )(η5 -C5 H5 ) (9b), Au(PPh3 ) (10b)) and [M](C[triple bond, length as m-dash]C-10-C14 H8 -9-NO2 ) ([M] = trans-[RuCl(dppe)2 ] (6c), trans-[RuCl(dppm)2 ] (7c), Ru(PPh3 )2 (η5 -C5 H5 ) (8c), Ni(PPh3 )(η5 -C5 H5 ) (9c), Au(PPh3 ) (10c)) were synthesized and their identities were confirmed by single-crystal X-ray diffraction studies...
March 7, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Tracy Bell, Magali Araujo, Zaiming Luo, James Tomlinson, James Leiper, William J Welch, Christopher S Wilcox
BACKGROUND: Nitric oxide prevents hypertension yet paradoxically reduces proximal tubule Na+ reabsorption. Nitric oxide synthase is inhibited by asymmetric dimethylarginine (ADMA) that is metabolized by dimethylarginine dimethylaminohydrolase (DDAH) whose type 1 isoform is expressed abundantly in the PT. HYPOTHESIS: That ADMA generated by DDAH-1 fluid reabsorbtion (Jv) by the proximal tubule. METHODS: S2 segments of the PT were perfused in vivo to assess Jv...
March 7, 2018: American Journal of Physiology. Renal Physiology
Ammar Oumaima, Ajina Tesnim, Haouas Zohra, Sallem Amira, Zidi Ines, Chakroun Sana, Grissa Intissar, Ezzi Lobna, Jlali Ali, Mehdi Meriem
DNA fragmentation can be deleterious on spermatozoon morphology but the pathogenesis of teratozoospermia associated with DNA breaks is not fully understood, even if oxidative attacks and defects in chromatin maturation are hypothesized. Therefore, this study is one of the first to clarify on the underlying hypothesizes behind such observations. The objectives of our study were to assess the role of oxidative attacks in DNA damage pathogenesis in ejaculated spermatozoa from patients with isolated teratozoospermia...
March 5, 2018: Environmental Science and Pollution Research International
Masakazu Kotoda, Tadahiko Ishiyama, Kazuha Mitsui, Sohei Hishiyama, Takashi Matsukawa
PURPOSE: Nicorandil has dual properties and acts as a nitric oxide donor and an ATP-sensitive potassium (KATP ) channel opener. Considering its pharmacological profile, nicorandil might exert protective effects on the brain as well as on the heart. The purpose of this study was to directly evaluate the effect of nicorandil on cerebral blood flow (CBF) in mice using a transcranial Doppler method. METHODS: Under general anesthesia, the nicorandil groups received a single-bolus intraperitoneal injection of the respective doses of nicorandil (1, 5, or 10 mg/kg), while the control group received vehicle only...
March 5, 2018: Journal of Anesthesia
Ido Azuri, Anna Hirsch, Anthony M Reilly, Alexandre Tkatchenko, Shai Kendler, Oded Hod, Leeor Kronik
We present a computational analysis of the terahertz spectra of the monoclinic and the orthorhombic polymorphs of 2,4,6-trinitrotoluene. Very good agreement with experimental data is found when using density functional theory that includes Tkatchenko-Scheffler pair-wise dispersion interactions. Furthermore, we show that for these polymorphs the theoretical results are only weakly affected by many-body dispersion contributions. The absence of dispersion interactions, however, causes sizable shifts in vibrational frequencies and directly affects the spatial character of the vibrational modes...
2018: Beilstein Journal of Organic Chemistry
Safak Bulut, Wendy L Queen
The formation of C-N bonds with aryl amines is one of the most widely studied reactions in organic chemistry. Despite this, it is still highly challenging often requiring expensive, precious metal-based catalysts. Here we report an easy catalyst-free methodology for constructing C-N bonds. The method, which proceeds via the in situ formation of closed ring amidinium ions, allows the preparation of a series of symmetrical and/or unsymmetrical aryl diamines in notably high yields (82-98 %) and purity and with a variety of different substituents...
March 5, 2018: Journal of Organic Chemistry
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