Read by QxMD icon Read

Sperm mix

E Malama, Y Zeron, F Janett, M Siuda, Z Roth, H Bollwein
Seasonal fluctuations of climate are considered a major factor affecting spermatogenesis and semen quality in the bovine. Our study aimed to investigate the effect of season on functional parameters of frozen-thawed bovine semen using computer-assisted sperm analysis (CASA) and flow cytometry. For this purpose, 86 ejaculates were collected from five mature Holstein-Friesian bulls kept under subtropical conditions during summer (August to September; n = 43) and winter (December to January; n = 43) months. Semen was diluted with a Tris-egg yolk-based extender and frozen at -196 °C...
August 25, 2016: Theriogenology
Lidia Mínguez-Alarcón, Oleg Sergeyev, Jane S Burns, Paige L Williams, Mary M Lee, Susan A Korrick, Luidmila Smigulina, Boris Revich, Russ Hauser
BACKGROUND: Exposures to endocrine disrupting chemicals during critical phases of testicular development may be related to poorer semen parameters. However, few studies have assessed the association between childhood organochlorine (OC) exposure and adult semen parameters. OBJECTIVE: We examined whether peripubertal serum OC concentrations are associated with semen parameters among young Russian men. METHODS: From 2003 to 2005, 516 boys were enrolled at age 8-9 years and followed for up to ten years...
October 7, 2016: Environmental Health Perspectives
Andrea Hesser, Christa Darr, Kris Gonzales, Heather Power, Tawny Scanlan, James Thompson, Charles Love, Bruce Christensen, Stuart Meyers
Semen quality in dogs has not been assessed in a longitudinal study that includes endpoints of female fertility and pregnancy. Although use of artificial insemination with chilled semen is increasingly used in canine reproduction, the resultant level of predictability and odds of fertile matings for dogs is still not fully understood. This research provides, for the first time, comprehensive semen evaluation in a large population of dogs in which fertility has been tracked. Duplicate ejaculates were obtained from 39 Labrador retriever males of the Guide Dogs for the Blind (San Rafael, CA, USA) breeding program...
August 20, 2016: Theriogenology
Pilar Santolaria, Alfredo Pauciullo, Miguel A Silvestre, Sandra Vicente-Fiel, Leyre Villanova, Alain Pinton, Juan Viruel, Ester Sales, Jesús L Yániz
This study was designed to determine the ability of computer-assisted sperm morphometry analysis (CASA-Morph) with fluorescence to discriminate between spermatozoa carrying different sex chromosomes from the nuclear morphometrics generated and different statistical procedures in the bovine species. The study was divided into two experiments. The first was to study the morphometric differences between X- and Y-chromosome-bearing spermatozoa (SX and SY, respectively). Spermatozoa from eight bulls were processed to assess simultaneously the sex chromosome by FISH and sperm morphometry by fluorescence-based CASA-Morph...
September 13, 2016: Asian Journal of Andrology
David J Sharkey, Kelton P Tremellen, Nancy E Briggs, Gustaaf A Dekker, Sarah A Robertson
STUDY QUESTION: Do seminal plasma transforming growth factor-β (TGFB) cytokines vary within individuals over time, and does this relate to sperm parameters, age or prior abstinence? SUMMARY ANSWER: Activin A and follistatin, and to a lesser extent TGFB1, TGFB2 and TGFB3, vary within individuals over time, in association with duration of abstinence. WHAT IS ALREADY KNOWN: Seminal plasma TGFB cytokines can influence sperm function and reproductive success through interactions with the female reproductive tract after coitus...
October 2016: Human Reproduction
Meritxell Jodar, Ada Soler-Ventura, Rafael Oliva
: Semen is a complex body fluid containing an admixture of spermatozoa suspended in secretions from the testes and epididymis which are mixed at the time of ejaculation with secretions from other accessory sex glands such as the prostate and seminal vesicles. High-throughput technologies have revealed that, contrary to idea that sperm cells are simply a silent delivery vehicle of the male genome to the oocyte, the sperm cells in fact provide both a specific epigenetically marked DNA together with a complex population of proteins and RNAs crucial for embryogenesis...
August 26, 2016: Journal of Proteomics
Feiby L Nassan, Brent A Coull, Niels E Skakkebaek, Michelle A Williams, Ramace Dadd, Lidia Mínguez-Alarcón, Stephen A Krawetz, Elizabeth J Hait, Joshua R Korzenik, Alan C Moss, Jennifer B Ford, Russ Hauser
BACKGROUND: Phthalates are widely used chemicals with ubiquitous exposure. Dibutyl-phthalate (DBP), a male reproductive toxicant in animals, is understudied in humans. Some mesalamine medications used to treat inflammatory bowel disease (IBD) have DBP in their coating, whereas other mesalamine formulations do not. OBJECTIVES: Taking advantage of differences in mesalamine formulations, we investigated whether high-DBP exposure from mesalamine medications was associated with decreased semen parameters...
October 2016: Environment International
W Maalouf, W Maalouf, B Campbell, K Jayaprakasan
OBJECTIVE: To evaluate the effect of ethnicity of women on the outcome of in vitro fertilisation (IVF) or intracytoplasmic sperm injection (ICSI) treatment. DESIGN: Observational cohort study. SETTING: UK National Database. POPULATION: Data from 2000 to 2010 involving 38 709 women undergoing their first IVF/ICSI cycle were analysed. METHODS: Anonymous data were obtained from the Human Fertilization and Embryology Authority (HFEA), the statutory regulator of IVF and ICSI treatment in the UK...
August 19, 2016: BJOG: An International Journal of Obstetrics and Gynaecology
Yunusa A Wada, Sonnie J Oniye, Peter I Rekwot, Oluyinka O Okubanjo
AIM: The study was conducted to evaluate the pathological effects of trypanosomosis on the testes, gonadal, and epididymal sperm reserves of Yankasa rams for 98 days. MATERIALS AND METHODS: A total of 16 Yankasa rams, aged between 24 and 30 months and weighed between 22 and 25 kg, were acclimatized for a period of 2-months in a clean fly proof house and were adequately fed and given water ad-libitum. Of the 16 rams, 12 that were clinically fit for the experiment at the end of the acclimatization period were randomly divided into four groups: Groups I, II, III, and IV, each having 3 rams...
July 2016: Veterinary World
Yu Chang, Ding-Hui Dai, Yuan Li, Yan Zhang, Ming Zhang, Guang-Bin Zhou, Chang-Jun Zeng
Mammalian spermatozoa gradually mature and acquire fertility during the transition from the testis to the caput and cauda epididymis, after which they are stored at the tail of the epididymis and the ampulla of vas deferens. During ejaculation, mixing of spermatozoa with the secretions of accessory sex glands leads to their dilution and changes in their function. Although remarkable progress has been made toward the understanding of changes in spermatozoa biochemistry and function before and after ejaculation, it is unknown whether microRNAs (miRNAs) are involved in regulating the function of spermatozoa during the transition between the cauda epididymis and ejaculation...
July 21, 2016: Theriogenology
Kristin M Wall, William Kilembe, Bellington Vwalika, Preeti Ravindhran, Naw Htee Khu, Ilene Brill, Elwyn Chomba, Brent A Johnson, Lisa B Haddad, Amanda Tichacek, Susan Allen
BACKGROUND: Evidence on the association between female-to-male human immunodeficiency virus (HIV) transmission risk and hormonal contraception is sparse and conflicting. METHODS: Heterosexual HIV-discordant couples from Lusaka, Zambia, were followed longitudinally at 3 month-intervals from 1994 to 2012. The impact of hormonal contraception on time to HIV transmission from HIV-positive women to their HIV-negative male partners (M-F+) was evaluated. RESULTS: Among 1601 M-F+ couples, 171 genetically linked HIV transmissions occurred in men over 3216 couple-years (5...
October 1, 2016: Journal of Infectious Diseases
Analía Risso, Francisco Javier Pellegrino, Alejandro Enrique Relling, Yanina Corrada
BACKGROUND: Manipulating the dietary fatty acid (FA) content can alter FA profiles of reproductive tissues. Numerous researchers have evaluated the effect of fish oil (FO) supplementation on reproductive characteristics in domestic animals, but reliable information concerning dietary FO effects on semen quality and testosterone concentrations in dogs has not been reported. Therefore, this study evaluated the effects of dietary FO on semen quality and serum testosterone concentrations in dogs...
July 2016: International Journal of Fertility & Sterility
Yi-chun Zheng, Jia-ying Liang, Peng Du, Guo-zhu Mo, Li-hu Wang, Feng-hua Liu
OBJECTIVE: To investigate the influence of different methods of semen preservation and processing on sperm DNA integrity. METHODS: We collected semen samples from 100 normozoospermic male volunteers and, following homogeneous mixing, preserved them by means of snap freezing, slow freezing, or at the room temperature for 4 and 24 hours. Meanwhile we processed the semen by washing, swim-up, and density gradient centrifugation (DGC). Then we obtained the sperm DNA fragmentation index (DFI) by sperm chromatin dispersion test and measured total sperm motility and DFI after cultured for 24 hours following processing...
May 2016: Zhonghua Nan Ke Xue, National Journal of Andrology
T T Dong, Q Yu, X R Qing, X L Ma, W W Dong, J Shi, H G Li
Cell-free seminal RNA (cfs-RNA) is mixed transcripts derived from male reproductive organs, and is potential biomarker for the research and diagnosis of male reproductive-related diseases. However, some clinical factors, including age, asymptomatic Ureaplasma urealyticum (UU) infection, scrotal heat stress, abstinence period, and the storage condition of semen samples, may interfere with sperm parameters and the measurement of seminal biomarkers. Accordingly, this study was designed to evaluate the effect of above clinical factors on the measurement of cfs-RNA, aiming to lay a foundation for its research use and potential clinical application...
July 1, 2016: Andrology
Maryam Bahmanzadeh, Aliasghar Vahidinia, Shayesteh Mehdinejadiani, Saeed Shokri, Zohreh Alizadeh
OBJECTIVE: Diabetes mellitus (DM) is known to cause many systemic complications as well as male infertility. Astaxanthin (ASTX) is a powerful antioxidant that is involved in a variety of biologically active processes, including those with anti-diabetes effects. The present study investigates the effect of ASTX on the spermatozoa function in streptozotocin (STZ)-induced diabetic rats. METHODS: We divided 30 adult rats into three groups (10 rats per group), with a control group that received corn oil mixed with chow...
June 2016: Clinical and Experimental Reproductive Medicine
Luke Simon, Armand Zini, Alina Dyachenko, Antonio Ciampi, Douglas T Carrell
Sperm DNA damage is prevalent among infertile men and is known to influence natural reproduction. However, the impact of sperm DNA damage on assisted reproduction outcomes remains controversial. Here, we conducted a meta-analysis of studies on sperm DNA damage (assessed by SCSA, TUNEL, SCD, or Comet assay) and clinical pregnancy after IVF and/or ICSI treatment from MEDLINE, EMBASE, and PUBMED database searches for this analysis. We identified 41 articles (with a total of 56 studies) including 16 IVF studies, 24 ICSI studies, and 16 mixed (IVF + ICSI) studies...
June 24, 2016: Asian Journal of Andrology
Christa R Darr, Dickson D Varner, Sheila Teague, Gino A Cortopassi, Sandipan Datta, Stuart A Meyers
Stallion sperm rely primarily on oxidative phosphorylation for production of ATP used in sperm motility and metabolism. The objective of the study was to identify which substrates included in Biggers, Whitten, and Whittingham (BWW) media are key to optimal mitochondrial function through measurements of sperm motility parameters, mitochondrial oxygen consumption, and cellular reactive oxygen species (ROS) production. It was expected that mitochondrial substrates, pyruvate and lactate, would support sperm motility and mitochondrial function better than the glycolytic substrate, glucose, due to direct utilization within the mitochondria...
August 2016: Biology of Reproduction
Manoharanehru Branavan, Ruth E Mackay, Pascal Craw, Angel Naveenathayalan, Jeremy C Ahern, Tulasi Sivanesan, Chris Hudson, Thomas Stead, Jessica Kremer, Neha Garg, Mark Baker, Syed T Sadiq, Wamadeva Balachandran
This paper presents the design of a modular point of care test platform that integrates a proprietary sample collection device directly with a microfluidic cartridge. Cell lysis, within the cartridge, is conducted using a chemical method and nucleic acid purification is done on an activated cellulose membrane. The microfluidic device incorporates passive mixing of the lysis-binding buffers and sample using a serpentine channel. Results have shown extraction efficiencies for this new membrane of 69% and 57% compared to the commercial Qiagen extraction method of 85% and 59...
August 2016: Medical Engineering & Physics
Luísa Camacho, Sherry M Lewis, Michelle M Vanlandingham, Beth E Juliar, Greg R Olson, Ralph E Patton, Gonçalo Gamboa da Costa, Kellie Woodling, Estatira Sepehr, Matthew S Bryant, Daniel R Doerge, Mallikarjuna S Basavarajappa, Robert P Felton, K Barry Delclos
Diet is an important variable in toxicology. There are mixed reports on the impact of soy components on energy utilization, fat deposition, and reproductive parameters. Three generations of CD-1 mice were fed irradiated natural ingredient diets with varying levels of soy (NIH-41, 5K96, or 5008/5001), purified irradiated AIN-93 diet, or the AIN-93 formulation modified with ethanol-washed soy protein concentrate (SPC) or SPC with isoflavones (SPC-IF). NIH-41 was the control for pairwise comparisons. Minimal differences were observed among natural ingredient diet groups...
August 2016: Food and Chemical Toxicology
Lu Zhang, Mei Ding, Hao Pang, Jiaxin Xing, Jinfeng Xuan, Chunhong Wang, Ziqing Lin, Song Han, Kewei Liang, Chunmei Li, Jun Yao, Baojie Wang
The identification of individuals in a mixture of two semen samples usually involves an analysis of autosomal and Y chromosomal short tandem repeats (STR) which can exclude unrelated individuals but cannot achieve the purpose of individual identification. In sperm cells, there are multiple copies of mitochondrial DNAs (mtDNA) which exhibit genetic polymorphisms in different matrilineal-related individuals. Single-cell capture technology can be applied to obtain some single sperm cells in a mixed semen sample, then polymerase chain reaction can be employed to amplify the mtDNA hypervariable region I (HVR I) from each cell...
August 2016: Electrophoresis
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"