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https://www.readbyqxmd.com/read/29352487/disaccharide-combinations-and-the-expression-of-enolase3-and-plasma-membrane-ca2-atpase-isoform-in-sturgeon-sperm-cryopreservation
#1
M D Xi, P Li, H Du, X M Qiao, Z G Liu, Q W Wei
Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary. Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality. We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus. We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A...
January 19, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29331832/investigations-on-a-cryopreservation-protocol-for-long-term-storage-of-psittacine-spermatozoa-using-cockatiel-semen-as-an-example
#2
H Schneider, D Fischer, K Failing, C Ehling, S Meinecke-Tillmann, A Wehrend, M Lierz
The aim of the present study was the establishment of an effective protocol for cryopreservation of psittacine semen. Therefore, pooled semen samples of 30 cockatiels (Nymphicus hollandicus) were diluted with modified Lake diluent (1:4), partitioned into four equal parts. Three portions were mixed with three cryoprotectants (dimethylacetamide, dimethyl sulfoxide, glycerol) in 4%, 8% and 12% final concentration, respectively, whereas the 4rth part served as control. Altogether, 96 incremental diluted semen samples were obtained for investigation...
December 16, 2017: Theriogenology
https://www.readbyqxmd.com/read/29320852/exposures-to-atmospheric-pm10-and-pm10-2-5-affect-male-semen-quality-results-of-marhcs-study
#3
Niya Zhou, Changtan Jiang, Qing Chen, Huan Yang, Xiaogang Wang, Peng Zou, Lei Sun, Jiaojiao Liu, Ling Li, Lianbing Li, Linping Huang, Hongqiang Chen, Lin Ao, Ziyuan Zhou, Jinyi Liu, Zhihong Cui, Jia Cao
Studies have shown that the effects of ambient particulate matter (PM) may be related to particle's size. However, results on the relationships between different PM and reproductive health are controversial. To explore the impacts of various PM fractions on male reproductive health, a total of 796 eligible subjects recruited in 2013 baseline investigation. In addition, there were 656 (82.4%) and 568 (71.3%) subjects participated follow-up surveys in 2014 and 2015, respectively. We used multivariable regression analysis and mixed-effect model to investigate the associations between air pollutants PM10, PM10-2...
January 11, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29304256/dynamics-ultrastructure-and-gene-expression-of-human-in-vitro-organized-testis-cells-from-testicular-sperm-extraction-biopsies
#4
Kathrein von Kopylow, Wolfgang Schulze, Andrea Salzbrunn, Matthias Schaks, Elke Schäfer, Beate Roth, Stefan Schlatt, Andrej-Nikolai Spiess
STUDY QUESTION: Is it possible to induce in-vitro reorganization of primary human testis cells from testicular sperm extraction (TESE) biopsies, maintain their long-term cultivation in a 2D system and identify cellular compositions? SUMMARY ANSWER: In-vitro reorganization of primary human testis cells from TESE biopsies and their long-term cultivation on uncoated cell culture dishes is feasible and the cellular compositions can be uncovered through gene expression and microscopic analyses...
January 3, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29244565/do-sperm-really-compete-and-do-eggs-ever-have-a-choice-adult-distribution-and-gamete-mixing-influence-sexual-selection-sexual-conflict-and-the-evolution-of-gamete-recognition-proteins-in-the-sea
#5
Don R Levitan
The evolution of gametic compatibility and the effectiveness of compatibility, within and across species, depend on whether sperm from different males directly compete for an egg and whether eggs ever have a choice. Direct sperm competition and egg choice depend on whether sperm from different males arrive at an egg in the brief interval between first sperm contact and fertilization. Although this process may be relevant for all sexually reproducing organisms, it is most easily examined in aquatic external fertilizers...
January 2018: American Naturalist
https://www.readbyqxmd.com/read/29241080/paternal-urinary-concentrations-of-organophosphate-flame-retardant-metabolites-fertility-measures-and-pregnancy-outcomes-among-couples-undergoing-in-vitro-fertilization
#6
Courtney C Carignan, Lidia Mínguez-Alarcón, Paige L Williams, John D Meeker, Heather M Stapleton, Craig M Butt, Thomas L Toth, Jennifer B Ford, Russ Hauser
BACKGROUND: Use of organophosphate flame retardants (PFRs) has increased over the past decade following the phase out of some brominated flame retardants, leading to increased human exposure. We recently reported that increasing maternal PFR exposure is associated with poorer pregnancy outcomes among women from a fertility clinic. Because a small epidemiologic study previously reported an inverse association between male PFR exposures and sperm motility, we sought to examine associations of paternal urinary concentrations of PFR metabolites and their partner's pregnancy outcomes...
December 11, 2017: Environment International
https://www.readbyqxmd.com/read/29238561/precopulatory-but-not-postcopulatory-male-reproductive-traits-diverge-in-response-to-mating-system-manipulation-in-drosophila-melanogaster
#7
Kristina U Wensing, Mareike Koppik, Claudia Fricke
Competition between males creates potential for pre- and postcopulatory sexual selection and conflict. Theory predicts that males facing risk of sperm competition should evolve traits to secure their reproductive success. If those traits are costly to females, the evolution of such traits may also increase conflict between the sexes. Conversely, under the absence of sperm competition, one expectation is for selection on male competitive traits to relax thereby also relaxing sexual conflict. Experimental evolution studies are a powerful tool to test this expectation...
December 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/29226264/generation-of-viable-progeny-from-dead-brooders-of-endangered-catfish-clarias-magur-hamilton-1822
#8
Sullip Kumar Majhi, Santosh Kumar
The obligatory air-breathing catfish Clarias magur is a prime candidate for aquaculture owing to its unique taste, high growth rate, and hardy nature. However, recently the IUCN has listed the species under the endangered category because the population has critically declined in the wild. The sexually mature C. magur brooders are often collected from their natural habitats for seed production in captivity. In many cases, the brooder dies due to handling injuries or confinement stress. In this study, we demonstrated that viable progeny could be generated from freshly dead sexually mature C...
October 2017: Heliyon
https://www.readbyqxmd.com/read/29221680/paradorippe-granulata-a-crab-with-external-fertilization-and-a-novel-type-of-sperm-storage-organ-challenges-prevalent-ideas-on-the-evolution-of-reproduction-in-eubrachyura-crustacea-brachyura-dorippidae
#9
Juliane Vehof, Gerhard Scholtz, Carola Becker
Two fundamentally different sperm storage organs occur in Brachyura. The probably paraphyletic podotremes show intersegmental spermathecae, which are distant from oviducts and coxal gonopores. Hence, fertilization is external. In contrast to this, the seminal receptacles of Eubrachyura are directly connected with the ovaries. Thus, at least initial fertilization is internal. This pattern has been interpreted as an apomorphy of Eubrachyura. To test this hypothesis, we studied the morphology of the reproductive organs of Paradorippe granulata, a representative of the putatively early diverging eubrachyuran lineage Dorippoidea...
December 5, 2017: Arthropod Structure & Development
https://www.readbyqxmd.com/read/29174895/a-peptide-signaling-system-that-rapidly-enforces-paternity-in-the-aedes-aegypti-mosquito
#10
Laura B Duvall, Nipun S Basrur, Henrik Molina, Conor J McMeniman, Leslie B Vosshall
Female Aedes aegypti mosquitoes typically mate only once with one male in their lifetime, a behavior known as "monandry" [1]. This single mating event provisions the female with sufficient sperm to fertilize the >500 eggs she will produce during her ∼4- to 6-week lifespan in the laboratory [2]. Successful mating induces lifetime refractoriness to subsequent insemination by other males, enforcing the paternity of the first male [3-5]. Ae. aegypti mate in flight near human hosts [6], and females become refractory to remating within seconds [1, 3, 4], suggesting the existence of a rapid mechanism to prevent female remating...
December 4, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/29172721/anogenital-distance-and-variability-in-semen-parameters
#11
José J López-Espín, Consuelo Pérez-Palazón, Ana B Maldonado-Cárceles, José D Román-Arias, Jaime Mendiola, Alberto M Torres-Cantero
The purpose of this study was to analyze whether the anogenital distance (AGD) was associated with variability in semen parameters. Semen parameters analyzed following the WHO guidelines and sperm DNA fragmentation were evaluated in 160 semen samples obtained over a period of a year from 16 healthy male volunteers. Two types of AGD measurements from the anus to the rear base of the scrotum (AGDAS) and to the cephalic insertion of the penis (AGDAP) were taken in each individual. The association between AGDs and semen parameters were studied using three statistical tools: a) general coefficient of variation (CV) and intra-individual coefficient of variation (CVi), b) general linear models for repeated measures, and c) mixed model fixed effects panel data...
November 27, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29155263/identification-and-characterisation-of-the-epididymal-proteins-in-the-lizard-eutropis-carinata-reptilia-squamata-schneider-1801
#12
R Medini, M Bhagya, S Samson
Lizards are seasonal breeders. Cyclic reproductive nature makes lizard as a useful model for the study of the reproductively active protein secretions in the epididymis. During breeding season, the epididymides of the lizard secret proteins that mixes with the spermatozoa and create a favourable environment for sperm maturation. In this spectrum, the aim of this study is to identify and characterize proteins which are present in the lumen of the epididymis of the lizard, E. carinata during the active phase of reproduction...
November 16, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29152252/effect-of-fish-oil-and-vitamin-e-on-sperm-lipid-peroxidation-in-dogs
#13
Analía Lorena Risso, Francisco J Pellegrino, Yanina Corrada, Mónica Marmunti, Mariana Gavazza, Alejandro Palacios, Alejandro E Relling
The objective was to evaluate the effects of dietary fish oil (FO) and vitamin E (VE) supplementation on sperm sensitivity to lipid peroxidation (LP) in dogs. Using an incomplete replicate 3 × 3 Latin square design, five dogs were allocated into three groups. One of the squares was incomplete and had two dogs that were used with three treatments. The dogs were assigned to three different treatments, fed a control diet of balanced commercial food (control group; CG), control diet supplemented with 54 mg FO/kg body weight(0·75) per d (FO group; FG) and FO plus 400 mg VE per d (FO and VE group; FEG) for 60 d...
2017: Journal of Nutritional Science
https://www.readbyqxmd.com/read/29128698/seminal-plasma-antioxidants-are-directly-involved-in-boar-sperm-cryotolerance
#14
Junwei Li, Isabel Barranco, Asta Tvarijonaviciute, Manuel F Molina, Emilio A Martinez, Heriberto Rodriguez-Martinez, Inmaculada Parrilla, Jordi Roca
Boar ejaculates are ejected in fractions with a specific composition in terms of sperm numbers and seminal plasma (SP), which is reflected in the varying sperm cryotolerance observed among different fractions. As boar sperm are particularly sensitive to oxidative stress, this study evaluated the role of SP antioxidants in the observed differences in sperm cryotolerance among ejaculate fractions. Ten ejaculates from five boars were manually collected in fractions: the first 10 mL of the sperm-rich fraction (SRF), the rest of the SRF and the post-SRF...
October 31, 2017: Theriogenology
https://www.readbyqxmd.com/read/29102416/hair-mercury-hg-levels-fish-consumption-and-semen-parameters-among-men-attending-a-fertility-center
#15
Lidia Mínguez-Alarcón, Myriam C Afeiche, Paige L Williams, Mariel Arvizu, Cigdem Tanrikut, Chitra J Amarasiriwardena, Jennifer B Ford, Russ Hauser, Jorge E Chavarro
General population exposure to methylmercury (MeHg), the most common organic mercury compound found in the environment, occurs primarily through the consumption of contaminated fish and shellfish. Due to limited studies and lack of consideration of effect modification by fish consumption, it remains uncertain if exposure to mercury affects semen parameters. Thus, we investigated whether hair Hg levels, a biomarker of mercury exposure, were associated with semen parameters among men attending an academic fertility center, and whether this relationship was modified by intake of fish...
October 28, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/29077936/urinary-concentrations-of-3-diethylcarbamoyl-benzoic-acid-dcba-a-major-metabolite-of-n-n-diethyl-m-toluamide-deet-and-semen-parameters-among-men-attending-a-fertility-center
#16
Thalia R Segal, Lidia Mínguez-Alarcón, Yu-Han Chiu, Paige L Williams, Feiby L Nassan, Ramace Dadd, María Ospina, Antonia M Calafat, Russ Hauser
STUDY QUESTION: Are specific gravity (SG)-adjusted urinary concentrations of 3-(diethylcarbamoyl)benzoic acid (DCBA) associated with semen parameters among men attending an academic fertility center? SUMMARY ANSWER: Our study did not demonstrate any association between SG-adjusted urinary DCBA concentrations and semen parameters among men attending an academic fertility center. WHAT IS KNOWN ALREADY: N,N-Diethyl-m-toluamide (DEET) is the most common active ingredient in consumer insect repellents...
October 25, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29073898/sexual-conflict-explains-the-extraordinary-diversity-of-mechanisms-regulating-mitochondrial-inheritance
#17
Arunas L Radzvilavicius, Nick Lane, Andrew Pomiankowski
BACKGROUND: Mitochondria are predominantly inherited from the maternal gamete, even in unicellular organisms. Yet an extraordinary array of mechanisms enforce uniparental inheritance, which implies shifting selection pressures and multiple origins. RESULTS: We consider how this high turnover in mechanisms controlling uniparental inheritance arises using a novel evolutionary model in which control of mitochondrial transmission occurs either during spermatogenesis (by paternal nuclear genes) or at/after fertilization (by maternal nuclear genes)...
October 26, 2017: BMC Biology
https://www.readbyqxmd.com/read/29035837/testicular-cytology-by-fine-needle-aspiration-in-domestic-cats
#18
D P Leme, E Visacre, V B Castro, M D Lopes
In cases where semen collection in tom-cats is not possible, FNA of testes is the alternative to evaluate sperm production. Although this technique for the diagnosis of fertility problems has been well discussed in other mammals (men, dogs, stallions), data for domestic cats are limited. Therefore, the aim of this study was to verify the reliability of FNA using needles of small diameters (22G and 29G) in testes of domestic cats of different ages to assess the spermatogenesis status and to present description of germ cells and Sertoli cells for cytological examinations...
October 9, 2017: Theriogenology
https://www.readbyqxmd.com/read/29021707/pectinase-treated-panax-ginseng-protects-against-chronic-intermittent-heat-stress-induced-testicular-damage-by-modulating-hormonal-and-spermatogenesis-related-molecular-expression-in-rats
#19
Spandana Rajendra Kopalli, Kyu-Min Cha, Sang-Ho Lee, Ji-Hoon Ryu, Seock-Yeon Hwang, Min-Sik Jeong, Jong-Hwan Sung, Si-Kwan Kim
BACKGROUND: Elevated testicular temperature disrupts spermatogenesis and causes infertility. In the present study, the protective effect of enzymatically biotransformed Panax ginseng Meyer by pectinase (GINST) against chronic intermittent heat stress-induced testicular damage in rats was investigated. METHODS: Male Sprague-Dawley rats (4 wk old, 60-70 g) were divided into four groups: normal control (NC), heat-stress control (HC), heat-stress plus GINST-100 mg/kg (HG100), and heat-stress plus GINST-200 mg/kg (HG200) treatment groups...
October 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28937752/inverse-association-between-ambient-sulfur-dioxide-exposure-and-semen-quality-in-wuhan-china
#20
Yuewei Liu, Yun Zhou, Jixuan Ma, Wei Bao, Jingjing Li, Ting Zhou, Xiuqing Cui, Zhe Peng, Hai Zhang, Min Feng, Yuan Yuan, Yuanqi Chen, Xiji Huang, Yonggang Li, Yonggang Duan, Tingming Shi, Lei Jin, Li Wu
Evidence concerning the association between ambient gaseous air pollutant exposures and semen quality is sparse, and findings in previous studies remain largely inconsistent. We enrolled 1759 men and performed 2184 semen examinations at a large reproductive medical center in Wuhan, China, between 2013 and 2015. Inverse distance weighting interpolation was performed to estimate individual exposures to SO2, NO2, CO, and O3 during the entire period (lag 0-90 days) and key periods (lag 0-9, 10-14, 70-90 days) of sperm development...
November 7, 2017: Environmental Science & Technology
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