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https://www.readbyqxmd.com/read/28078758/periconceptional-maternal-high-fish-and-olive-oil-low-meat-dietary-pattern-is-associated-with-increased-embryonic-growth-the-rotterdam-periconceptional-cohort-predict-study
#1
Francesca Parisi, Melek Rousian, Nicole A Huijgen, Anton H J Koning, Sten P Willemsen, Jeanne H M de Vries, Irene Cetin, Eric A P Steegers, Régine P M Steegers-Theunissen
OBJECTIVE: To investigate associations between periconceptional maternal dietary patterns and first trimester embryonic growth. METHODS: 228 women with singleton ongoing pregnancies were enrolled in a prospective periconceptional cohort study, comprising of 135 strictly dated spontaneous pregnancies and 93 pregnancies achieved after in vitro fertilization or intracytoplasmatic sperm injection (IVF/ICSI pregnancies). All women underwent longitudinal transvaginal three-dimensional ultrasound (3D-US) scans from 6(+0) up to 13(+0) weeks of gestation...
January 12, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28028016/multiple-and-asymmetrical-origin-of-polyploid-dog-rose-hybrids-rosa-l-sect-caninae-dc-ser-involving-unreduced-gametes
#2
V Herklotz, C M Ritz
BACKGROUND AND AIMS: Polyploidy and hybridization are important factors for generating diversity in plants. The species-rich dog roses (Rosa sect. Caninae) originated by allopolyploidy and are characterized by unbalanced meiosis producing polyploid egg cells (usually 4x) and haploid sperm cells (1x). In extant natural stands species hybridize spontaneously, but the extent of natural hybridization is unknown. The aim of the study was to document the frequency of reciprocal hybridization between the subsections Rubigineae and Caninae with special reference to the contribution of unreduced egg cells (5x) producing 6x offspring after fertilization with reduced (1x) sperm cells...
December 27, 2016: Annals of Botany
https://www.readbyqxmd.com/read/28012719/cysteamine-supplementation-revealed-detrimental-effect-on-cryosurvival-of-buffalo-sperm-based-on-computer-assisted-semen-analysis-and-oxidative-parameters
#3
Dheer Singh Swami, Pradeep Kumar, R K Malik, Monika Saini, Dharmendra Kumar, M H Jan
The aim of this study was to investigate the effect of addition of cysteamine to the semen extender on post-thaw semen quality. A total of 30 ejaculates were collected from six bulls. Each ejaculate was divided into five equal parts and diluted to final concentration of 80 million sperms/mL using Optixcell(®)(IMV, France) semen extender supplemented with different concentrations of cysteamine (0, 0.75, 1.25, 2.5 and 5mM) and cryopreserved. In the frozen-thawed samples, the VAP, VSL, VCL ALH and sperm motility of control samples was greater (P<0...
December 13, 2016: Animal Reproduction Science
https://www.readbyqxmd.com/read/27939508/time-lapse-morphokinetic-assessment-has-low-to-moderate-ability-to-predict-euploidy-when-patient-and-ovarian-stimulation-related-factors-are-taken-into-account-with-the-use-of-clustered-data-analysis
#4
Sezcan Mumusoglu, Irem Yarali, Gurkan Bozdag, Pınar Ozdemir, Mehtap Polat, Lale Karakoc Sokmensuer, Hakan Yarali
OBJECTIVE: To study whether time-lapse morphokinetic (TLM) assessment predicts ploidy status when patient- and ovarian stimulation-related factors are taken into account. DESIGN: Retrospective cohort study. SETTING: Private IVF clinic. PATIENT(S): In total, 103 consecutive patients (415 blastocysts) were included. All embryos were individually cultured in a time-lapse incubator from intracytoplasmic sperm injection up to trophectoderm biopsy...
December 6, 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27932246/denaturing-action-of-adjuvant-affects-specificity-of-polyclonal-antibodies
#5
K V Barinova, E V Khomyakova, M L Kuravsky, E V Schmalhausen, V I Muronetz
Influence of the immunization procedure on the specificity of the produced antibodies towards different conformations of the antigen was investigated. It was demonstrated that intravenous immunization of a rabbit with an adjuvant-free solution of recombinant sperm-specific glyceraldehyde-3-phosphate dehydrogenase (dN-GAPDS) resulted in production of antibodies recognizing only native conformation of dN-GAPDS and exhibiting no cross-reaction with somatic isoenzyme of glyceraldehyde-3-phosphate dehydrogenase...
December 5, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27888150/coenzyme-q10-intake-from-food-and-semen-parameters-in-a-subfertile-population
#6
Bruno C Tiseo, Audrey J Gaskins, Russ Hauser, Jorge E Chavarro, Cigdem Tanrikut
OBJECTIVE: To assess the association between coenzyme Q10 (CoQ10) intake from food sources and semen quality. We assessed this association in a prospective cohort of men attending a fertility clinic. CoQ10 supplementation has been associated with improvements in semen parameters. However, the impact of CoQ10 intake from food sources on semen quality has not been investigated. MATERIALS AND METHODS: Subfertile couples seeking fertility evaluation at the Massachusetts General Hospital Fertility Center were invited to participate in an ongoing study of environmental factors and fertility...
November 22, 2016: Urology
https://www.readbyqxmd.com/read/27876951/prevalence-of-dermanyssus-and-ornithonyssus-species-of-mites-in-poultry-farms-of-vikarabad-area-of-hyderabad
#7
G S Sreenivasa Murthy, Rasmita Panda
The common blood feeder mites of poultry are from the genera Dermanyssus and Ornithonyssus. Their presence are problematic for the producers either through potential direct effects on weight gain, egg production or sperm production in roosters or as nuisance pests on workers. They also cause anaemia in birds and play a vector role for several human and animal diseases. Five poultry farm buildings of Vikarabad area of Rangareddy district were visited. Samples were collected from a variety of sites, including beneath feed troughs, inside cage fittings and fastening clips, under egg conveyer belts and under manure belts...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27866723/association-between-ambient-particulate-matter-exposure-and-semen-quality-in-wuhan-china
#8
Li Wu, Lei Jin, Tingming Shi, Bing Zhang, Yun Zhou, Ting Zhou, Wei Bao, Hua Xiang, Yao Zuo, Guanlian Li, Cheng Wang, Yonggang Duan, Zhe Peng, Xiji Huang, Hai Zhang, Tian Xu, Yonggang Li, Xinyun Pan, Ying Xia, Xun Gong, Weihong Chen, Yuewei Liu
BACKGROUND: Health effects of exposure to particulate matter (PM) on male reproductive health remain unclear. Only a limited number of studies have investigated the effects of PM2.5 or PM10 exposure on semen quality, and the results were largely inconsistent. OBJECTIVES: To quantitatively assess the exposure-response association between PM exposure and semen quality in Chinese men who were exposed to a wide concentration range of PM. METHODS: We investigated 1759 men from Wuhan, China, who were partners of women undergoing assisted reproductive technology procedures, and had semen examined at least once between 2013 and 2015...
November 17, 2016: Environment International
https://www.readbyqxmd.com/read/27865449/characterizing-nuclear-and-mitochondrial-dna-in-spent-embryo%C3%A2-culture-media-genetic-contamination-identified
#9
Elizabeth R Hammond, Brent C McGillivray, Sophie M Wicker, John C Peek, Andrew N Shelling, Peter Stone, Larry W Chamley, Lynsey M Cree
OBJECTIVE: To characterize nuclear and mitochondrial DNA (mtDNA) in spent culture media from normally developing blastocysts to determine whether it could be used for noninvasive genetic assessment. DESIGN: Prospective embryo cohort study. SETTING: Academic center and private in vitro fertilization (IVF) clinic. PATIENT(S): Seventy patients undergoing intracytoplasmic sperm injection (ICSI) and 227 blastocysts. INTERVENTION(S): Culture media assessment, artificial blastocoele fluid collapse and DNA analysis using digital polymerase chain reaction (dPCR), long-range PCR, quantitative PCR (qPCR), and DNA fingerprinting...
January 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/27857155/fluorescence-and-magnetic-activated-cell-sorting-strategies-to-separate-spermatozoa-involving-plural-contributors-from-biological-mixtures-for-human-identification
#10
Yan Xu, Jianhui Xie, Ronghua Chen, Yu Cao, Yuan Ping, Qingwen Xu, Wei Hu, Dan Wu, Lihua Gu, Huaigu Zhou, Xin Chen, Ziqin Zhao, Jiang Zhong, Rui Li
No effective method has been developed to distinguish sperm cells originating from different men in multi-suspect sexual assault cases. Here we combined MACS and FACS to isolate single donor sperm cells from forensic mixture samples including female vaginal epithelial cells and sperm cells from multiple contributors. Sperms from vaginal swab were isolated by MACS using FITC-conjugated A kinase anchor protein 3 (AKAP3) antibody; target individual sperm cells involving two or three donors were separated by FACS using FITC-labeled blood group A/B antigen antibody...
November 18, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27809479/designing-micro-and-nanoswimmers-for-specific-applications
#11
Jaideep Katuri, Xing Ma, Morgan M Stanton, Samuel Sánchez
Self-propelled colloids have emerged as a new class of active matter over the past decade. These are micrometer sized colloidal objects that transduce free energy from their surroundings and convert it to directed motion. The self-propelled colloids are in many ways, the synthetic analogues of biological self-propelled units such as algae or bacteria. Although they are propelled by very different mechanisms, biological swimmers are typically powered by flagellar motion and synthetic swimmers are driven by local chemical reactions, they share a number of common features with respect to swimming behavior...
November 3, 2016: Accounts of Chemical Research
https://www.readbyqxmd.com/read/27800030/fertility-preservation-in-males-with-cancer-16-year-monocentric-experience-of-sperm-banking-and-post-thaw-reproductive-outcomes
#12
Raffaella Depalo, Doriana Falagario, Paola Masciandaro, Claudia Nardelli, Margherita Patrizia Vacca, Pasquale Capuano, Giorgina Specchia, Michele Battaglia
BACKGROUND: Anticancer treatments can impair male fertility. Cryopreservation of semen is an efficient procedure for fertility preservation. The aim of this study was to evaluate pre-freeze semen parameters among the various types of cancer, post-thaw sperm viability and reproductive outcome of samples used for assisted reproductive treatment (ART). METHODS: This study included 721 men with cancer that had their semen cryopreserved in our bank in 1999-2015. Semen analysis and cryopreservation were performed before the start of antineoplastic treatment, according to the World Health Organization recommendations, European Commission and Italian law...
November 2016: Therapeutic Advances in Medical Oncology
https://www.readbyqxmd.com/read/27720270/use-of-computer-assisted-sperm-analysis-and-flow-cytometry-to-detect-seasonal-variations-of-bovine-semen-quality
#13
E Malama, Y Zeron, F Janett, M Siuda, Z Roth, H Bollwein
Seasonal fluctuations of climate are considered a major factor affecting spermatogenesis and semen quality in the bovine. Our study aimed to investigate the effect of season on functional parameters of frozen-thawed bovine semen using computer-assisted sperm analysis (CASA) and flow cytometry. For this purpose, 86 ejaculates were collected from five mature Holstein-Friesian bulls kept under subtropical conditions during summer (August to September; n = 43) and winter (December to January; n = 43) months. Semen was diluted with a Tris-egg yolk-based extender and frozen at -196 °C...
January 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/27713107/a-longitudinal-study-of-peripubertal-serum-organochlorine-concentrations-and-semen-parameters-in-young-men-the-russian-children-s-study
#14
Lidia Mínguez-Alarcón, Oleg Sergeyev, Jane S Burns, Paige L Williams, Mary M Lee, Susan A Korrick, Luidmila Smigulina, Boris Revich, Russ Hauser
BACKGROUND: Exposures to endocrine disrupting chemicals during critical phases of testicular development may be related to poorer semen parameters. However, few studies have assessed the association between childhood organochlorine (OC) exposure and adult semen parameters. OBJECTIVE: We examined whether peripubertal serum OC concentrations are associated with semen parameters among young Russian men. METHODS: From 2003 to 2005, 516 boys were enrolled at age 8-9 years and followed for up to ten years...
October 7, 2016: Environmental Health Perspectives
https://www.readbyqxmd.com/read/27658745/semen-evaluation-and-fertility-assessment-in-a-purebred-dog%C3%A2-breeding-facility
#15
Andrea Hesser, Christa Darr, Kris Gonzales, Heather Power, Tawny Scanlan, James Thompson, Charles Love, Bruce Christensen, Stuart Meyers
Semen quality in dogs has not been assessed in a longitudinal study that includes endpoints of female fertility and pregnancy. Although use of artificial insemination with chilled semen is increasingly used in canine reproduction, the resultant level of predictability and odds of fertile matings for dogs is still not fully understood. This research provides, for the first time, comprehensive semen evaluation in a large population of dogs in which fertility has been tracked. Duplicate ejaculates were obtained from 39 Labrador retriever males of the Guide Dogs for the Blind (San Rafael, CA, USA) breeding program...
January 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/27624989/computer-assisted-sperm-morphometry-fluorescence-based-analysis-has-potential-to-determine-progeny-sex
#16
Pilar Santolaria, Alfredo Pauciullo, Miguel A Silvestre, Sandra Vicente-Fiel, Leyre Villanova, Alain Pinton, Juan Viruel, Ester Sales, Jesús L Yániz
This study was designed to determine the ability of computer-assisted sperm morphometry analysis (CASA-Morph) with fluorescence to discriminate between spermatozoa carrying different sex chromosomes from the nuclear morphometrics generated and different statistical procedures in the bovine species. The study was divided into two experiments. The first was to study the morphometric differences between X- and Y-chromosome-bearing spermatozoa (SX and SY, respectively). Spermatozoa from eight bulls were processed to assess simultaneously the sex chromosome by FISH and sperm morphometry by fluorescence-based CASA-Morph...
November 2016: Asian Journal of Andrology
https://www.readbyqxmd.com/read/27609985/seminal-plasma-transforming-growth-factor-%C3%AE-activin-a-and-follistatin-fluctuate-within-men-over-time
#17
David J Sharkey, Kelton P Tremellen, Nancy E Briggs, Gustaaf A Dekker, Sarah A Robertson
STUDY QUESTION: Do seminal plasma transforming growth factor-β (TGFB) cytokines vary within individuals over time, and does this relate to sperm parameters, age or prior abstinence? SUMMARY ANSWER: Activin A and follistatin, and to a lesser extent TGFB1, TGFB2 and TGFB3, vary within individuals over time, in association with duration of abstinence. WHAT IS ALREADY KNOWN: Seminal plasma TGFB cytokines can influence sperm function and reproductive success through interactions with the female reproductive tract after coitus...
October 2016: Human Reproduction
https://www.readbyqxmd.com/read/27576136/semen-proteomics-and-male-infertility
#18
Meritxell Jodar, Ada Soler-Ventura, Rafael Oliva
: Semen is a complex body fluid containing an admixture of spermatozoa suspended in secretions from the testes and epididymis which are mixed at the time of ejaculation with secretions from other accessory sex glands such as the prostate and seminal vesicles. High-throughput technologies have revealed that, contrary to idea that sperm cells are simply a silent delivery vehicle of the male genome to the oocyte, the sperm cells in fact provide both a specific epigenetically marked DNA together with a complex population of proteins and RNAs crucial for embryogenesis...
August 26, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27575365/a-crossover-crossback-prospective-study-of-dibutyl-phthalate-exposure-from-mesalamine-medications-and-semen-quality-in-men-with-inflammatory-bowel-disease
#19
Feiby L Nassan, Brent A Coull, Niels E Skakkebaek, Michelle A Williams, Ramace Dadd, Lidia Mínguez-Alarcón, Stephen A Krawetz, Elizabeth J Hait, Joshua R Korzenik, Alan C Moss, Jennifer B Ford, Russ Hauser
BACKGROUND: Phthalates are widely used chemicals with ubiquitous exposure. Dibutyl-phthalate (DBP), a male reproductive toxicant in animals, is understudied in humans. Some mesalamine medications used to treat inflammatory bowel disease (IBD) have DBP in their coating, whereas other mesalamine formulations do not. OBJECTIVES: Taking advantage of differences in mesalamine formulations, we investigated whether high-DBP exposure from mesalamine medications was associated with decreased semen parameters...
October 2016: Environment International
https://www.readbyqxmd.com/read/27539893/effect-of-ethnicity-on-live-birth-rates-after-in-vitro-fertilisation-intracytoplasmic-sperm-injection-treatment-analysis-of-uk-national-database
#20
W Maalouf, W Maalouf, B Campbell, K Jayaprakasan
OBJECTIVE: To evaluate the effect of ethnicity of women on the outcome of in vitro fertilisation (IVF) or intracytoplasmic sperm injection (ICSI) treatment. DESIGN: Observational cohort study. SETTING: UK National Database. POPULATION: Data from 2000 to 2010 involving 38 709 women undergoing their first IVF/ICSI cycle were analysed. METHODS: Anonymous data were obtained from the Human Fertilization and Embryology Authority (HFEA), the statutory regulator of IVF and ICSI treatment in the UK...
August 19, 2016: BJOG: An International Journal of Obstetrics and Gynaecology
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