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https://www.readbyqxmd.com/read/29232835/expression-profile-of-genes-regulating-steroid-biosynthesis-and-metabolism-in-human-ovarian-granulosa-cells-a-primary-culture-approach
#1
Wiesława Kranc, Maciej Brązert, Katarzyna Ożegowska, Mariusz J Nawrocki, Joanna Budna, Piotr Celichowski, Marta Dyszkiewicz-Konwińska, Maurycy Jankowski, Michal Jeseta, Leszek Pawelczyk, Małgorzata Bruska, Michał Nowicki, Maciej Zabel, Bartosz Kempisty
Because of the deep involvement of granulosa cells in the processes surrounding the cycles of menstruation and reproduction, there is a great need for a deeper understanding of the ways in which they function during the various stages of those cycles. One of the main ways in which the granulosa cells influence the numerous sex associated processes is hormonal interaction. Expression of steroid sex hormones influences a range of both primary and secondary sexual characteristics, as well as regulate the processes of oogenesis, folliculogenesis, ovulation, and pregnancy...
December 9, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29231996/molecular-mechanisms-of-tributyltin-induced-alterations-in-cholesterol-homeostasis-and-steroidogenesis-in-hamster-testis-in-vivo-and-in-vitro-studies
#2
V Kanimozhi, K Palanivel, M A Akbarsha, B Kadalmani
Tributyltins (TBT) are ubiquitous and persistent environmental contaminants that disturb normal endocrine function including gonadal function in humans and marine organisms. TBT was administered through oral route to male Syrian hamsters at daily doses of 50, 100 and 150 ppm/kg for 65 days. Changes in testis morphology, immunohistochemistry of iNOS, 3β-HSD and 17β-HSD, cholesterol transport receptor, nuclear receptors, and transcription factors were analyzed. TBT treatment affected each of these parameters to significant levels in a dose-dependent manner compared to vehicle treated control...
December 12, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29229458/the-effects-of-irrigants-on-the-survival-of-human-stem-cells-of-the-apical-papilla-including-endocyn
#3
Mark B Scott, Gregory S Zilinski, Timothy C Kirkpatrick, Van T Himel, Kent A Sabey, Thomas E Lallier
INTRODUCTION: Endocyn, a pH-neutral solution of hypochlorous acid and hypochlorite has been developed for use as an endodontic irrigant. The purpose of this study was to evaluate the effect of Endocyn on human periodontal ligament (PDL) fibroblasts, rat osteosarcoma cells (UMR-106), and stem cells of the apical papilla (SCAP) compared with other commonly used endodontic irrigants. METHODS: To determine cytotoxicity, cells were exposed to various concentrations of Endocyn, 6% sodium hypochlorite (NaOCl), 17% EDTA, and 2% chlorhexidine for 10 minutes, 1 hour, or 24 hours...
December 8, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/29217199/efficient-generation-of-transgene-and-feeder-free-induced-pluripotent-stem-cells-from-human-dental-mesenchymal-stem-cells-and-their-chemically-defined-differentiation-into-cardiomyocytes
#4
Xiaobing Tan, Qingli Dai, Tao Guo, Jingshu Xu, Qingyuan Dai
Advance in stem cell research resulted in several processes to generate induced pluripotent stem cells (iPSCs) from adult somatic cells. In our previous study, the reprogramming of iPSCs from human dental mesenchymal stem cells (MSCs) including SCAP and DPSCs, has been reported. Herein, safe iPSCs were reprogrammed from SCAP and DPSCs using non-integrating RNA virus vector, which is an RNA virus carrying no risk of altering host genome. DPSCs- and SCAP-derived iPSCs exhibited the characteristics of the classical morphology with human embryonic stem cells (hESCs) without integration of foreign genes, indicating the potential of their clinical application...
December 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29206289/contiguous-verrucous-proliferations-in-syringocystadenoma-papilliferum-a-retrospective-analysis-with-additional-evaluation-via-mutation-specific-braf-v600e-immunohistochemistry
#5
B J Friedman, J Sahu, C C Solomides, D M Connolly, J B Lee
Syringocystadenoma papilliferum (SCAP) is a benign adnexal neoplasm with apocrine differentiation.1,2 which may be derived from pluripotent cells.3 The lesion may develop within a pre-existing nevus sebaceus, but the majority of cases arise sporadically.3 Congenital lesions in a Blaschkoid distribution have also been reported, and these cases are thought to represent genetic mosaicism.4,5,6 The tumor has a varied clinical appearance, but typically presents as a raised warty, hairless plaque or nodule.3.
December 5, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/29148982/a-dentin-derived-hydrogel-bioink-for-3d-printing-of-cell-laden-scaffolds-for-regenerative-dentistry
#6
Avathamsa Athirasala, Anthony Tahayeri, Greeshma Thrivikraman, Cristiane Miranda Franca, Nelson Monteiro, Victor Tran, Jack Ferracane, Luiz Bertassoni
Recent studies in tissue engineering have adopted extracellular matrix (ECM) derived scaffolds as natural and cytocompatible microenvironments for tissue regeneration. The dentin matrix, specifically, has been shown to be associated with a host of soluble and insoluble signaling molecules that can promote odontogenesis. Here, we have developed a novel bioink, blending printable alginate (3% w/v) hydrogels with the soluble and insoluble fractions of the dentin matrix. We have optimized the printing parameters and the concentrations of the individual components of the bioink for print accuracy, cell viability and odontogenic potential...
November 17, 2017: Biofabrication
https://www.readbyqxmd.com/read/29139614/human-dental-stem-cell-derived-transgene-free-ipscs-generate-functional-neurons-via-embryoid-body-mediated-and-direct-induction-methods
#7
Ikbale El Ayachi, Jun Zhang, Xiao-Ying Zou, Zongdong Yu, Wei Wei, Kristen M S O'Connell, George T-J Huang
Induced pluripotent stem cells (iPSCs) give rise to neural stem/progenitor cells (NSCs), serving as a good source for neural regeneration. Here, we established transgene-free (TF) iPSCs from dental stem cells (DSCs) and determined their capacity to differentiate into functional neurons in vitro. Generated TF iPSCs from stem cells of apical papilla (SCAP) and dental pulp stem cells (DPSCs) underwent two methods -- embryoid body (EB)-mediated and direct induction, to guide TF-DSC iPSCs along with H9 or H9 Syn-GFP (human embryonic stem cells) into functional neurons in vitro...
November 15, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29131204/voltage-dependent-admittance-spectroscopy-for-the-detection-of-near-interface-defect-states-for-thin-film-solar-cells
#8
Thomas Paul Weiss, Shiro Nishiwaki, Benjamin Bissig, Stephan Buecheler, Ayodhya N Tiwari
Recently recorded efficiencies of Cu(In,Ga)Se2 based solar cells were mainly achieved by surface treatment of the absorber that modifies the buffer-absorber interface region. However, only little is known about the electronic properties within this region. In this manuscript voltage dependent admittance spectroscopy is applied to low temperature grown Cu(In,Ga)Se2 based solar cells to detect near interface defect states in the absorber. Under non-equilibrium conditions even defect states close to the interface may cross the Fermi level and hence are detectable using capacitance based measurement methods, in contrast to the case of zero bias conditions...
November 22, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29118330/molecular-spectrum-of-secretome-regulates-the-relative-hepatogenic-potential-of-mesenchymal-stem-cells-from-bone-marrow-and-dental-tissue
#9
Ajay Kumar, Vinod Kumar, Vidya Rattan, Vivekananda Jha, Arnab Pal, Shalmoli Bhattacharyya
Liver regeneration is a spontaneous process that occurs after liver injury, but acute liver failure is a complex and fatal disease which is difficult to treat. Cell-based therapies are promising alternative therapeutic approach for liver failure and different cell sources have been tested in this regard. We investigated the comparative hepatogenic potential of human bone marrow stem cells (BMSC) with stem cells derived from human dental pulp (DPSC), apical papilla (SCAP) and follicle (DFSC) during this study...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29100611/saving-the-birds-oliver-l-austin-s-collaboration-with-japanese-scientists-in-revising-wildlife-policies-in-us-occupied-japan-1946-1950
#10
Annika A Culver
In postwar Tokyo, ornithologist Oliver L. Austin's leadership of the Wildlife Branch of the Natural Resources Section (NRS) for the Supreme Commander of the Allied Powers (SCAP) serves as an intriguing lens into the reconstruction of Japanese conservation activities. His experiences as a scientist working on wildlife policies in US-occupied Korea (1945-1946) and Japan (1946-1949) illuminate the war's impact on individuals and their environment. Austin collaborated closely with elite Japanese colleagues, despite their ruined laboratories, burnt collections, inadequate shelter, and despair...
October 31, 2017: Endeavour
https://www.readbyqxmd.com/read/29097707/gp73-regulates-hepatic-steatosis-by-enhancing-scap-srebps-interaction
#11
Xiaoli Yang, Feixiang Wu, Jiankang Chen, Cui Wang, Yongjie Zhu, Feng Li, Qinfang Hao, Cuijuan Duan, Li Wang, Xueping Ma, Deyong Zou, Li Luo, Yiwen Zhao, Kai Guan, Yuan Cao, Pingping Zhang, Pengyu Zhou, Shengli Ma, Zhifeng Yan, Jia Li, Yanhong Zhang, Congwen Wei, Hui Zhong
Elevated Golgi phosphoprotein 2 (GP73, also known as GOLPH2 or GOLM1) expression in serum and liver, which can be induced by viral infection and cytokine treatments, is intimately connected with liver disease, including acute hepatitis, cirrhosis and hepatocellular carcinoma (HCC). However, its pathogenic roles in hepatic diseases have never been clarified in detail. Here, we showed that the upregulated GP73 is indispensable for SREBPs activation and lipogenesis. Notably, GP73 overexpression enhanced SCAP-SREBPs binding and its Golgi trafficking even under cholesterol sufficiency...
November 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29068315/inhibition-of-cholesterol-biosynthesis-through-rnf145-dependent-ubiquitination-of-scap
#12
Li Zhang, Prashant Rajbhandari, Christina Priest, Jaspreet Sandhu, Xiaohui Wu, Ryan Temel, Antonio Castrillo, Thomas Q de Aguiar Vallim, Tamer Sallam, Peter Tontonoz
Cholesterol homeostasis is maintained through concerted action of the SREBPs and LXRs. Here, we report that RNF145, a previously uncharacterized ER membrane ubiquitin ligase, participates in crosstalk between these critical signaling pathways. RNF145 expression is induced in response to LXR activation and high-cholesterol diet feeding. Transduction of RNF145 into mouse liver inhibits the expression of genes involved in cholesterol biosynthesis and reduces plasma cholesterol levels. Conversely, acute suppression of RNF145 via shRNA-mediated knockdown, or chronic inactivation of RNF145 by genetic deletion, potentiates the expression of cholesterol biosynthetic genes and increases cholesterol levels both in liver and plasma...
October 25, 2017: ELife
https://www.readbyqxmd.com/read/29053036/eyelid-syringocystadenoma-papilliferum-a-novel-presentation-with-major-review
#13
Michael C Tseng, Bijal Amin, Anne Barmettler
A major review of the literature of syringocystadenoma papilliferum's (SCAP's) presentation and management is presented. In addition, a case report of this unique diagnosis presenting as a corneal abrasion in a 66-year-old-male is included. This benign adnexal tumor of the apocrine glands is most commonly found in the face and neck. When found on the eyelids, these lesions are commonly misdiagnosed as basal cell carcinoma or cysts. Diagnosis is made based on histopathology. Treatment is complete excision of the lesion and this has a low recurrence rate...
October 20, 2017: Orbit
https://www.readbyqxmd.com/read/29018483/the-angiogenic-potential-of-dpscs-and-scaps-in-an-in-vivo-model-of-dental-pulp-regeneration
#14
Petra Hilkens, Annelies Bronckaers, Jessica Ratajczak, Pascal Gervois, Esther Wolfs, Ivo Lambrichts
Adequate vascularization, a restricting factor for the survival of engineered tissues, is often promoted by the addition of stem cells or the appropriate angiogenic growth factors. In this study, human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAPs) were applied in an in vivo model of dental pulp regeneration in order to compare their regenerative potential and confirm their previously demonstrated paracrine angiogenic properties. 3D-printed hydroxyapatite scaffolds containing DPSCs and/or SCAPs were subcutaneously transplanted into immunocompromised mice...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28986711/blockade-of-lgr4-inhibits-proliferation-and-odonto-osteogenic-differentiation-of-stem-cells-from-apical-papillae
#15
Meng Zhou, Shuyu Guo, Lichan Yuan, Yuxin Zhang, Mengnan Zhang, Huimin Chen, Mengting Lu, Jianrong Yang, Junqing Ma
During tooth root development, stem cells from apical papillae (SCAPs) are indispensable, and their abilities of proliferation, migration and odontoblast differentiation are linked to root formation. Leucine-rich repeat-containing GPCR 4 (LGR4) modulates the biological processes of proliferation and differentiation in multiple stem cells. In this study, we showed that LGR4 is expressed in all odontoblast cell lineage cells and Hertwig's epithelial root sheath (HERS) during the mouse root formation in vivo. In vitro we determined that LGR4 is involved in the Wnt/β-catenin signaling pathway regulating proliferation and odonto/osteogenic differentiation of SCAPs...
December 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28974767/the-neurotrophic-effects-of-different-human-dental-mesenchymal-stem-cells
#16
Mallappa K Kolar, Vinay N Itte, Paul J Kingham, Lev N Novikov, Mikael Wiberg, Peyman Kelk
The current gold standard treatment for peripheral nerve injury is nerve grafting but this has disadvantages such as donor site morbidity. New techniques focus on replacing these grafts with nerve conduits enhanced with growth factors and/or various cell types such as mesenchymal stem cells (MSCs). Dental-MSCs (D-MSCs) including stem cells obtained from apical papilla (SCAP), dental pulp stem cells (DPSC), and periodontal ligament stem cells (PDLSC) are potential sources of MSCs for nerve repair. Here we present the characterization of various D-MSCs from the same human donors for peripheral nerve regeneration...
October 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28947064/impact-of-pre-incubation-time-of-silk-fibroin-scaffolds-in-culture-medium-on-cell-proliferation-and-attachment
#17
Mehdi Amirikia, Seyed Mohammad Ali Shariatzadeh, Seyed Gholam Ali Jorsaraei, Malek Soleimani Mehranjani
Cell behaviours such as proliferation and attachment can be affected by the length of pre-incubation period of the scaffolds in the culture medium for long term. The aim of this study was to investigate the long term pre-incubation of 3D silk fibroin scaffolds in complete culture medium on cell attachment and proliferation. After the preparation of silk fibroin scaffolds by the technique of freeze drying, the scaffolds were pre-incubated in complete culture medium for 2 d, 6 d or 10 d before apical papilla stem cells (SCAP) seeding...
September 20, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28901252/the-origin-and-identification-of-mesenchymal-stem-cells-in-teeth-from-odontogenic-to-non-odontogenic
#18
Dixin Cui, Hongyu Li, Mian Wan, Yiran Peng, Xin Xu, Xuedong Zhou, Liwei Zheng
Mesenchymal stem cells (MSCs) in teeth have been exploited as vital seed cells for stem cell-based dental medicine. To date, several mesenchymal stem cell populations originated from odontogenic tissue have been isolated and characterized by their expression of MSC surface markers and capacity of multi-lineage differentiation, including dental pulp stem cells (DPSCs), stem cells from human exfoliated deciduous teeth (SHED), stem cells from apical papilla (SCAP) and so on. However, their identity in vivo remains elusive, which hinders further understanding of their application in stem cell-based tooth regeneration...
September 13, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28894386/oxidative-stress-in-patients-with-endodontic-pathologies
#19
Veiko Vengerfeldt, Reet Mändar, Mare Saag, Anneli Piir, Tiiu Kullisaar
BACKGROUND: Apical periodontitis (AP) is an inflammatory disease affecting periradicular tissues. It is a widespread condition but its etiopathogenetic mechanisms have not been completely elucidated and the current treatment options are not always successful. PURPOSE: To compare oxidative stress (OxS) levels in the saliva and the endodontium (root canal [RC] contents) in patients with different endodontic pathologies and in endodontically healthy subjects. PATIENTS AND METHODS: The study group of this comparison study included 22 subjects with primary chronic apical periodontitis (pCAP), 26 with posttreatment or secondary chronic apical periodontitis (sCAP), eight with acute periapical abscess, 13 with irreversible pulpitis, and 17 healthy controls...
2017: Journal of Pain Research
https://www.readbyqxmd.com/read/28883496/overexpression-of-insig-2-inhibits-atypical-antipsychotic-induced-adipogenic-differentiation-and-lipid-biosynthesis-in-adipose-derived-stem-cells
#20
Chien-Chih Chen, Li-Wen Hsu, Kuang-Tzu Huang, Shigeru Goto, Chao-Long Chen, Toshiaki Nakano
Atypical antipsychotics (AAPs) are considered to possess superior efficacy for treating both the positive and negative symptoms of schizophrenia; however, AAP use often causes excessive weight gain and metabolic abnormalities. Recently, several reports have demonstrated that AAPs activate sterol regulatory element-binding protein (SREBP). SREBP, SREBP cleavage-activating protein (SCAP) and insulin-induced gene (Insig) regulate downstream cholesterol and fatty acid biosynthesis. In this study, we explored the effects of clozapine, olanzapine and risperidone on SREBP signaling and downstream lipid biosynthesis genes in the early events of adipogenic differentiation in adipose-derived stem cells (ASCs)...
September 7, 2017: Scientific Reports
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