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https://www.readbyqxmd.com/read/28229417/a-simplified-and-systematic-method-to-isolate-culture-and-characterize-multiple-types-of-human-dental-stem-cells-from-a-single-tooth
#1
Mohammed Bakkar, Younan Liu, Dongdong Fang, Camille Stegen, Xinyun Su, Murali Ramamoorthi, Li-Chieh Lin, Takako Kawasaki, Nicholas Makhoul, Huan Pham, Yoshinori Sumita, Simon D Tran
This chapter describes a simplified method that allows the systematic isolation of multiple types of dental stem cells such as dental pulp stem cells (DPSC), periodontal ligament stem cells (PDLSC), and stem cells of the apical papilla (SCAP) from a single tooth. Of specific interest is the modified laboratory approach to harvest/retrieve the dental pulp tissue by minimizing trauma to DPSC by continuous irrigation, reduction of frictional heat from the bur rotation, and reduction of the bur contact time with the dentin...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28228593/er-protein-scap-inhibits-dengue-virus-ns2b3-protease-by-suppressing-its-k27-linked-polyubiquitylation
#2
Heng Liu, Lele Zhang, Jin Sun, Wei Chen, Senlin Li, Qiang Wang, Huansha Yu, Zanxian Xia, Xia Jin, Chen Wang
Dengue viruses (DENVs) are an emerging threat to global public health. The NS2B3 protease complex of DENV has recently been shown to cleave antiviral protein STING and thereby subverting the innate immune signaling to facilitate virus replication. Whether host cells have mechanism to counteract this virus-mediated immunosuppression is unclear. We discovered that the K27-linked poly-ubiquitination of NS3 protein facilitates its recruitment of NS2B and the formation of NS2B3, and consequently the enhanced cleavage of STING...
February 22, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28219188/-change-of-prognosis-and-pathogens-in-adult-patients-with-severe-community-acquired-pneumonia-during-different-periods
#3
L Zhang, P Ning, F Rong, Z C Gao
Objective: To compare the change of prognosis and pathogens of adult patients with severe community-acquired pneumonia (CAP) requiring admission to Respiratory Intensive Care Unit (RICU) during different period, and estimate the risk factors of severe CAP. Methods: The clinical data of 186 patients with severe CAP admission to RICU was collected and assessed the trend in outcomes of these patients during three time periods: Period Ⅰ (January 1 2000 to October 31 2006), Period Ⅱ (November 1 2006 to December 31 2010), and Period Ⅲ (January 1 2011 to December 31 2015), analyzed the risk factors by Cox Proportional Hazards regression, and compared the pathogens detection...
February 7, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28207658/association-between-paraspinal-muscle-morphology-clinical-symptoms-and-functional-status-in-patients-with-degenerative-cervical-myelopathy
#4
Maryse Fortin, Octavian Dobrescu, Matthew Courtemanche, Carolyn J Sparrey, Carlo Santaguida, Michael G Fehlings, Michael H Weber
STUDY DESIGN: A cross-sectional study. OBJECTIVE: The aim of this study was to assess fatty infiltration and asymmetry of the multifidus (MF), semispinalis cervicis (SCer), semispinalis capitis (SCap), and splenius capitis (SPL) muscles in patients with degenerative cervical myelopathy (DCM), and evaluate their correlations with clinical symptoms and functional scores. SUMMARY OF BACKGROUND DATA: Cervical muscle alterations have been reported in patients with chronic neck pain, but the assessment of cervical muscle morphology has been overlooked in patients with DCM...
February 15, 2017: Spine
https://www.readbyqxmd.com/read/28196330/decellularized-human-dental-pulp-as-a-scaffold-for-regenerative-endodontics
#5
J S Song, K Takimoto, M Jeon, J Vadakekalam, N B Ruparel, A Diogenes
Teeth undergo postnatal organogenesis relatively late in life and only complete full maturation a few years after the crown first erupts in the oral cavity. At this stage, development can be arrested if the tooth organ is damaged by either trauma or caries. Regenerative endodontic procedures (REPs) are a treatment alternative to conventional root canal treatment for immature teeth. These procedures rely on the transfer of apically positioned stem cells, including stem cells of the apical papilla (SCAP), into the root canal system...
February 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28190588/survival-of-the-apical-papilla-and-its-resident-stem-cells-in-a-case-of-advanced-pulpal-necrosis-and-apical-periodontitis
#6
Vanessa Chrepa, Brandon Pitcher, Michael A Henry, Anibal Diogenes
INTRODUCTION: Apical papilla represents a source of an enriched mesenchymal stem cell (MSC) population (stem cells of the apical papilla [SCAPs]) that modulates root development and may participate in regenerative endodontic procedures in immature teeth with pulp necrosis. The characteristics and phenotype of this tissue in the presence of inflammation are largely unknown. The purpose of this study was to characterize a human apical papilla sample that was isolated from an immature tooth with pulp necrosis and apical periodontitis...
February 9, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28168742/astrocyte-lipid-metabolism-is-critical-for-synapse-development-and-function-in-vivo
#7
Anne-Lieke F van Deijk, Nutabi Camargo, Jaap Timmerman, Tim Heistek, Jos F Brouwers, Floriana Mogavero, Huibert D Mansvelder, August B Smit, Mark H G Verheijen
The brain is considered to be autonomous in lipid synthesis with astrocytes producing lipids far more efficiently than neurons. Accordingly, it is generally assumed that astrocyte-derived lipids are taken up by neurons to support synapse formation and function. Initial confirmation of this assumption has been obtained in cell cultures, but whether astrocyte-derived lipids support synapses in vivo is not known. Here, we address this issue and determined the role of astrocyte lipid metabolism in hippocampal synapse formation and function in vivo...
February 7, 2017: Glia
https://www.readbyqxmd.com/read/28164259/five-year-mortality-in-patients-treated-for-severe-community-acquired-pneumonia-a-retrospective-study
#8
H Lenz, G O Norby, V Dahl, T E Ranheim, R E Haagensen
BACKGROUND: The mortality rate in patients with severe community-acquired pneumonia (SCAP) is high. We investigated the 5-year mortality rate and causes of death in a patient population treated for SCAP in our intensive care unit (ICU), and compared the mortality rate in patients with or without chronic obstructive pulmonary disease (COPD) as comorbidity. METHODS: This retrospective study, which covers a period of 10 years, included patients aged > 18 years admitted to our ICU with SCAP as primary diagnosis and in need of mechanical ventilation for more than 24 h...
February 5, 2017: Acta Anaesthesiologica Scandinavica
https://www.readbyqxmd.com/read/28145066/analysis-of-gene-expression-profiles-between-apical-papilla-tissues-stem-cells-from-apical-papilla-and-cell-sheet-to-identify-the-key-modulators-in-mscs-niche
#9
Shu Diao, Xiao Lin, Liping Wang, Rui Dong, Juan Du, Dongmei Yang, Zhipeng Fan
OBJECTIVES: The microenvironmental niche plays the key role for maintaining the cell functions. The stem cells from apical papilla (SCAPs) are important for tooth development and regeneration. However, there is limited knowledge about the key factors in niche for maintaining the function of SCAPs. In this study, we analyse the gene expression profiles between apical papilla tissues, SCAPs and SCAPs cell sheet to identify the key genes in SCAPs niche. MATERIALS AND METHODS: Microarray assays and bioinformatic analysis were performed to screen the differential genes between apical papilla tissues and SCAPs, and SCAPs and SCAPs cell sheet...
February 1, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28132894/vitamin-d-metabolite-25-hydroxyvitamin-d-regulates-lipid-metabolism-by-inducing-degradation-of-srebp-scap
#10
Lisa Asano, Mizuki Watanabe, Yuta Ryoden, Kousuke Usuda, Takuya Yamaguchi, Bilon Khambu, Megumi Takashima, Shin-Ichi Sato, Juro Sakai, Kazuo Nagasawa, Motonari Uesugi
Sterol regulatory element-binding proteins (SREBPs) are transcription factors that control lipid homeostasis. SREBP activation is regulated by a negative feedback loop in which sterols bind to SREBP cleavage-activating protein (SCAP), an escort protein essential for SREBP activation, or to insulin-induced genes (Insigs) (endoplasmic reticulum [ER] anchor proteins), sequestering the SREBP-SCAP-Insig complex in the ER. We screened a chemical library of endogenous molecules and identified 25-hydroxyvitamin D (25OHD) as an inhibitor of SREBP activation...
January 23, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28123479/three-single-nucleotide-polymorphisms-associated-with-type-2-diabetes-mellitus-in-a-chinese-population
#11
Meijun Chen, Xuelong Zhang, Qingxiao Fang, Tongtong Wang, Tingting Li, Hong Qiao
An Indian study recently observed three new loci: rs9552911 in the SGCG, rs1593304 near PLXNA4 and rs4858889 in SCAP associated with type 2 diabetes mellitus (T2DM) in a south Asian population. The present study aimed to validate these findings in a Chinese population. We genotyped the above three single-nucleotide polymorphisms (SNPs), rs9552911, rs1593304, and rs4858889, in a group of 1,972 Chinese individuals, comprising of 966 type 2 diabetic patients and 976 controls. Anthropometric variables and biochemical traits were measured in all the participants...
January 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28122233/feedback-loop-regulation-of-scap-srebp-1-by-mir-29-modulates-egfr-signaling-driven-glioblastoma-growth
#12
Peng Ru, Peng Hu, Feng Geng, Xiaokui Mo, Chunming Cheng, Ji Young Yoo, Xiang Cheng, Xiaoning Wu, Jeffrey Yunhua Guo, Ichiro Nakano, Etienne Lefai, Balveen Kaur, Arnab Chakravarti, Deliang Guo
No abstract text is available yet for this article.
January 24, 2017: Cell Reports
https://www.readbyqxmd.com/read/28116873/the-scap-srebp-pathway-a-mediator-of-hepatic-steatosis
#13
REVIEW
Young Ah Moon
Nonalcoholic fatty liver disease (NAFLD) is strongly associated with insulin resistance, obesity, and dyslipidemia. NAFLD encompasses a wide range of states from the simple accumulation of triglycerides in the hepatocytes to serious states accompanied by inflammation and fibrosis in the liver. De novo lipogenesis has been shown to be a significant factor in the development of hepatic steatosis in insulin-resistant states. Sterol regulatory element binding protein-1c (SREBP-1c) is the main transcription factor that mediates the activation of lipogenesis, and SREBP cleavage activating protein (SCAP) is required for the activation of SREBPs...
January 19, 2017: Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28064055/the-antioxidative-and-hepatoprotective-effects-comparison-of-chinese-angelica-polysaccharide-cap-and-selenizing-cap-scap-in-ccl4-induced-hepatic-injury-mice
#14
Zhenzhen Gao, Chao Zhang, Weijun Tian, Kuanhui Liu, Ranran Hou, Chanjuan Yue, Yi Wu, Deyun Wang, Jiaguo Liu, Yuanliang Hu, Ying Yang
Chinese angelica polysaccharides (CAP) and selenizing CAP (sCAP) were prepared and identified through FTIR and SEM observation. Their antioxidant activities in vitro and hepatoprotective effects in vivo were compared by free radical-scavenging tests or with CCl4-induced hepatic injury model mice. The results showed that for DPPH radical, superoxide anion and hydroxyl radical, the scavenging capabilities of sCAP were significantly stronger than those of CAP. In hepatic injury model mice, sCAP could significantly reduce ALT, AST and ALP contents and raised TP content in serum, significantly reduce MDA and ROS contents and raised SOD and T-AOC activities in liver homogenate in comparison with CAP; obviously relieve the pathological changes of liver and significantly inhibit the expressions of p-ERK, p-JNK and p-p38 protein as compared with those in model control group...
April 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28041686/evaluation-of-a-commercially-available-hyaluronic-acid-hydrogel-restylane-as-injectable-scaffold-for-dental-pulp-regeneration-an-in%C3%A2-vitro-evaluation
#15
Vanessa Chrepa, Obadah Austah, Anibal Diogenes
INTRODUCTION: Regenerative endodontic procedures (REPs) are viable alternatives for treating immature teeth, yet these procedures do not predictably lead to pulp-dentin regeneration. A true bioengineering approach for dental pulp regeneration requires the incorporation of a scaffold conducive with the regeneration of the pulp-dentin complex. Several materials have been proposed as scaffolds for REPs; nonetheless, the majority are not eligible for immediate clinical chairside use. Thus, the aim of this study was to evaluate Restylane, a Food and Drug Administration-approved hyaluronic acid-based gel, as possible scaffold for REPs...
December 29, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/28033156/tubulopapillary-cystic-adenoma-with-apocrine-differentiation-a-unifying-concept-for-syringocystadenoma-papilliferum-apocrine-gland-cyst-and-tubular-papillary-adenoma
#16
Shin-Ichi Ansai, Takashi Anan, Takaya Fukumoto, Hidehisa Saeki
Syringocystadenoma papilliferum (SCAP), apocrine gland cyst (AGC, also called apocrine hidrocystoma or apocrine cystadenoma), and tubular papillary adenoma (TPA) with apocrine differentiation are defined as proliferations of apocrine epithelium with myoepithelial cells. At Sapporo Dermatopathology Institute, we retrieved 308 benign neoplastic lesions diagnosed as SCAP, AGC, or TPA and combinations of these entities. Among the 308 lesions, 202 (66%) exhibited features of only one type, of which 144 (47%) were AGC, 39 (13%) were TPA, and 19 (6%) were SCAP...
December 28, 2016: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/28027595/inhibition-of-the-srebp-pathway-suppresses-hepatocellular-carcinoma-through-repressing-inflammation
#17
Na Li, Zhang-Sen Zhou, Yang Shen, Jie Xu, Hong-Hua Miao, Ying Xiong, Feng Xu, Bo-Liang Li, Jie Luo, Bao-Liang Song
: Obesity is a critical risk factor for hepatocellular carcinoma (HCC). However, it remains unknown whether inhibition of de novo lipid biosynthesis can suppress HCC. In this study, we blocked the sterol regulatory element binding protein (SREBP) pathway, one of the key determinants of lipid homeostasis, by ablating gp78 or SCAP in hepatocytes as well as by administering a chemical compound called betulin. We found that either genetically or pharmacologically inhibiting the SREBP pathway dramatically reduced diethylnitrosamine (DEN)-induced HCC progression through down-regulating tumor promoting cytokines including IL-6, TNF-α and IL-1β...
December 27, 2016: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28003358/heat-shock-protein-90-modulates-lipid-homeostasis-by-regulating-the-stability-and-function-of-sterol-regulatory-element-binding-protein-srebp-and-srebp-cleavage-activating-protein
#18
Yen-Chou Kuan, Tsutomu Hashidume, Takahiro Shibata, Koji Uchida, Makoto Shimizu, Jun Inoue, Ryuichiro Sato
Sterol regulatory element-binding proteins (SREBPs) are the key transcription factors that modulate lipid biosynthesis. SREBPs are synthesized as endoplasmic reticulum-bound precursors that require proteolytic activation in the Golgi apparatus. The stability and maturation of precursor SREBPs depend on their binding to SREBP cleavage-activating protein (SCAP), which escorts the SCAP-SREBP complex to the Golgi apparatus. In this study, we identified heat shock protein (HSP) 90 as a novel SREBP regulator that binds to and stabilizes SCAP-SREBP...
February 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27939739/characterization-of-a-vascular-endothelial-growth-factor-loaded-bioresorbable-delivery-system-for-pulp-regeneration
#19
Mamatha Yadlapati, Claudia Biguetti, Franco Cavalla, Francisco Nieves, Christopher Bessey, Pedram Bohluli, Gustavo Pompermaier Garlet, Ariadne Letra, Walid D Fakhouri, Renato Menezes Silva
INTRODUCTION: Vascular endothelial growth factor (VEGF) is a signal protein that stimulates angiogenesis and vasculogenesis and has been used in tissue regeneration and pulp regeneration experimental models. The purpose of this study was to develop a delivery system composed of a biodegradable fiber and controlled release of VEGF to promote cell viability and secure an adequate blood supply for the survival of human stem cells of the apical papilla (SCAP) favoring endodontic regenerative procedures...
January 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/27918898/roles-of-l-type-calcium-channels-cav1-2-and-the-distal-c-terminus-dct-in-differentiation-and-mineralization-of-rat-dental-apical-papilla-stem-cells-rscaps
#20
Qianqian Gao, Jianping Ge, Yanqin Ju, Changsheng Li, Jian Gao, Meisheng Wu, Shouliang Zhao
OBJECTIVE: Voltage-gated inward Ca(2+) currents (ICa) are triggered by cell depolarization and commonly produce transient increases in the cytoplasmic free Ca(2+) concentration. The CaV1.2 distal C-terminus is susceptible to proteolytic cleavage, which yields a truncated CaV1.2 subunit and a cleaved C-terminal fragment (CCt or DCT). Stem cells from the apical papilla (SCAPs) has a capacity for differentiation into the odontoblastic-like cells in vitro and dentin forming in vivo, which makes SCAPs advantages in tissue engineering and regenerative endodontic...
February 2017: Archives of Oral Biology
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