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https://www.readbyqxmd.com/read/28822568/effects-of-lipopolysaccharide-on-the-proliferation-and-osteogenic-differentiation-of-stem-cells-from-the-apical-papilla
#1
Veera Lertchirakarn, Panuroot Aguilar
INTRODUCTION: Stem cells from the apical papilla (SCAPs) were suggested as the stem cell source in regenerative endodontic procedures. However, bone and/or cementum-like structure were observed in root canals. Lipopolysaccharide (LPS) in infected root canals might alter SCAPs' osteogenic differentiation pattern. The objectives of this study were to investigate the effects of LPS on SCAPs' proliferation and osteogenic differentiation. METHODS: The mesenchymal stem cell characteristics of SCAPs were confirmed...
August 16, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28794794/sfrp2-enhances-the-osteogenic-differentiation-of-apical-papilla-stem-cells-by-antagonizing-the-canonical-wnt-pathway
#2
Luyuan Jin, Yu Cao, Guoxia Yu, Jinsong Wang, Xiao Lin, Lihua Ge, Juan Du, Liping Wang, Shu Diao, Xiaomeng Lian, Songlin Wang, Rui Dong, Zhaochen Shan
BACKGROUND: Exploring the molecular mechanisms underlying directed differentiation is helpful in the development of clinical applications of mesenchymal stem cells (MSCs). Our previous study on dental tissue-derived MSCs demonstrated that secreted frizzled-related protein 2 (SFRP2), a Wnt inhibitor, could enhance osteogenic differentiation in stem cells from the apical papilla (SCAPs). However, how SFRP2 promotes osteogenic differentiation of dental tissue-derived MSCs remains unclear...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28757246/evaluation-of-mesenchymal-stem-cell-modulation-of-trigeminal-neuronal-responses-to-cold
#3
Michael A Eskander, Koyo Takimoto, Anibal Diogenes
Tissue engineering protocols, such as regenerative endodontic procedures (REPs), comprise biologically based procedures designed to restore normal physiologic function. For REPs, the goal is reconstitution of the pulp-dentin complex by delivering mesenchymal stem cells (MSCs), including the stem cells of the apical papilla (SCAP) into a root canal system. Many patients regain cold sensitivity after REPs, but the mechanism is not understood. We hypothesized that SCAP modulate nociceptive function through a paracrine mechanism that activates cold-sensitive ion channels in neurons...
July 27, 2017: Neuroscience
https://www.readbyqxmd.com/read/28736205/developmental-and-extrahepatic-physiological-functions-of-srebp-pathway-genes-in-mice
#4
REVIEW
Luke J Engelking, Mary Jo Cantoria, Yanchao Xu, Guosheng Liang
Sterol regulatory element-binding proteins (SREBPs), master transcriptional regulators of cholesterol and fatty acid synthesis, have been found to contribute to a diverse array of cellular processes. In this review, we focus on genetically engineered mice in which the activities of six components of the SREBP gene pathway, namely SREBP-1, SREBP-2, Scap, Insig-1, Insig-2, or Site-1 protease have been altered through gene knockout or transgenic approaches. In addition to the expected impacts on lipid metabolism, manipulation of these genes in mice is found to affect a wide array of developmental and physiologic processes ranging from interferon signaling in macrophages to synaptic transmission in the brain...
July 20, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28734036/-epicatechin-regulates-blood-lipids-and-attenuates-hepatic-steatosis-in-rats-fed-high-fat-diet-%C3%A2
#5
Hui Cheng, Na Xu, Wenxia Zhao, Jingjing Su, Mengru Liang, Zhongwen Xie, Xianli Wu, Qinglin Li
SCOPE: (-)-Epicatechin (EC) is a natural flavanol monomer found in cocoa, green tea and a variety of other plant foods. In this study, effects of EC on blood lipids and hepatic steatosis, and the underlying mechanisms were investigated. METHODS AND RESULTS: A hyperlipidemic rat model was induced by high-fat, high-cholesterol diet. EC was then administrated to the animals by gavage at doses of 10 mg/kg, 20 mg/kg, 40 mg/kg body weight (BW) for 12 weeks. Simvastatin was included as a positive control...
July 21, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28725049/loss-of-calreticulin-uncovers-a-critical-role-for-calcium-in-regulating-cellular-lipid-homeostasis
#6
Wen-An Wang, Wen-Xin Liu, Serpen Durnaoglu, Sun-Kyung Lee, Jihong Lian, Richard Lehner, Joohong Ahnn, Luis B Agellon, Marek Michalak
A direct link between Ca(2+) and lipid homeostasis has not been definitively demonstrated. In this study, we show that manipulation of ER Ca(2+) causes the re-distribution of a portion of the intracellular unesterified cholesterol to a pool that is not available to the SCAP-SREBP complex. The SREBP processing pathway in ER Ca(2+) depleted cells remained fully functional and responsive to changes in cellular cholesterol status but differed unexpectedly in basal activity. These findings establish the role of Ca(2+) in determining the reference set-point for controlling cellular lipid homeostasis...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28675011/-characterization-of-micrornas-profiles-of-induced-pluripotent-stem-cells-reprogrammed-from-human-dental-pulp-stem-cells-and-stem-cells-from-apical-papilla
#7
Tan Xiaobing, Dai Qingyuan
OBJECTIVE: To compare characterization of microRNAs (miRNAs) expression profiles of induced pluripotent stem cells (iPSCs) reprogrammed from human dental pulp stem cells (DPSCs) and stem cells from apical papilla (SCAP) and screen-specific microRNA. METHODS: Human DPSCs and SCAP were reprogrammed into iPSCs using a Sendai virus vector. Total RNA of human DPSCs-iPSCs and SCAP-iPSCs were extracted. miRNAs were labeled and hybridized. Slides were scanned, and images were imported into GenePix Pro 6...
June 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28664184/microrna-29-mediates-a-novel-negative-feedback-loop-to-regulate-scap-srebp-1-and-lipid-metabolism
#8
Peng Ru, Deliang Guo
The membrane-bound transcription factors, SREBPs (sterol regulatory element-binding proteins), play a central role in regulating lipid metabolism. The transcriptional activation of SREBPs requires the key protein SCAP (SREBP-cleavage activating protein) to translocate their precursors from the endoplasmic reticulum to the Golgi for subsequent proteolytic activation, a process tightly regulated by a cholesterol-mediated negative feedback loop. Our previous work showed that the SCAP/SREBP-1 pathway is significantly upregulated in human glioblastoma (GBM), the most deadly brain cancer, and that glucose-mediated N-glycosylation of SCAP is a prerequisite step for SCAP/SREBP trafficking...
2017: RNA & Disease
https://www.readbyqxmd.com/read/28659153/advanced-glycation-end-products-ages-increase-renal-lipid-accumulation-a-pathogenic-factor-of-diabetic-nephropathy-dn
#9
Yang Yuan, Hong Sun, Zilin Sun
BACKGROUND: Advanced glycation end products (AGEs) are pathogenic factors of diabetic nephropathy (DN), causing renal damage in various ways. The aim of this study is to investigate the ectopic lipid accumulation caused by AGEs in human renal tubular epithelial cell line (HK-2) cells and the kidney of type 2 diabetic rats. METHODS: In vivo study, diabetes was induced in male Sprague-Dawley rats through intraperitoneal injection of high-fat/high-sucrose diet and low-dose streptozocin (STZ)...
June 28, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28649865/dental-stem-cells-recent-progresses-in-tissue-engineering-and-regenerative-medicine
#10
João Botelho, Maria Alzira Cavacas, Vanessa Machado, José João Mendes
Since the disclosure of adult mesenchymal stem cells (MSCs) there have been an intense investigation on the characteristics of these cells and their potentialities. Dental Stem Cells (DSCs) are MSC-like populations with self-renewal capacity and multidifferentiation potential. Currently, there are five main DSCs, dental pulp stem cells (DPSCs), stem cells from exfoliated deciduous teeth (SHED), stem cells from apical papilla (SCAP), periodontal ligament stem cells (PDLSCs), and Dental Follicle Precursor Cells (DFPCs)...
June 24, 2017: Annals of Medicine
https://www.readbyqxmd.com/read/28636235/stem-cell-based-tooth-and-periodontal-regeneration
#11
Lei Hu, Yi Liu, Songlin Wang
Currently regeneration of tooth and periodontal damage still remains great challenge. Stem cell-based tissue engineering raised novel therapeutic strategies for tooth and periodontal repair. Stem cells for tooth and periodontal regeneration include dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs), stem cells from the dental apical papilla (SCAPs), and stem cells from human exfoliated deciduous teeth (SHEDs), dental follicle stem cells (DFSCs), dental epithelial stem cells (DESCs), bone marrow mesenchymal stem cells (BMMSCs), adipose-derived stem cells (ADSCs), embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs)...
June 21, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28605223/homeobox-c10-inhibits-the-osteogenic-differentiation-potential-of-mesenchymal-stem-cells
#12
Guoqing Li, Nannan Han, Haoqing Yang, Liping Wang, Xiao Lin, Shu Diao, Juan Du, Rui Dong, Songlin Wang, Zhipeng Fan
PURPOSE: Mesenchymal stem cells (MSCs) are a reliable cell source for tissue regeneration. However, the molecular mechanisms underlying the directed differentiation of MSCs remain unclear which impedes potential clinical applications. Recent studies have discovered that HOX genes are involved in the differentiation regulation of MSCs and bone formation. In this study, we investigate the HOXC10 function in the osteogenic differentiation potential of MSCs. MATERIALS AND METHODS: Stem cells from apical papilla (SCAPs) and adipose-derived stem cells (ADSCs) were used in this study...
June 12, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28578888/comparative-evaluation-of-chemotactic-factor-effect-on-migration-and-differentiation-of-stem-cells-of-the-apical-papilla
#13
Sara Fayazi, Koyo Takimoto, Anibal Diogenes
INTRODUCTION: Cell homing strategies could potentially be used in regenerative endodontic procedures (REPs) to promote the progressive coronal migration of stem cells, including stem cells of the apical papilla (SCAPs), along with formation of a new vascular network without the need for intentional apical trauma and intracanal bleeding. Although many chemotactic factors have been investigated for different mesenchymal stem cells, their effect on SCAP migration and differentiation is not fully understood...
June 1, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28549068/oligodendroglial-myelination-requires-astrocyte-derived-lipids
#14
Nutabi Camargo, Andrea Goudriaan, Anne-Lieke F van Deijk, Willem M Otte, Jos F Brouwers, Hans Lodder, David H Gutmann, Klaus-Armin Nave, Rick M Dijkhuizen, Huibert D Mansvelder, Roman Chrast, August B Smit, Mark H G Verheijen
In the vertebrate nervous system, myelination of axons for rapid impulse propagation requires the synthesis of large amounts of lipids and proteins by oligodendrocytes and Schwann cells. Myelin membranes are thought to be cell-autonomously assembled by these axon-associated glial cells. Here, we report the surprising finding that in normal brain development, a substantial fraction of the lipids incorporated into central nervous system (CNS) myelin are contributed by astrocytes. The oligodendrocyte-specific inactivation of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP), an essential coactivator of the transcription factor SREBP and thus of lipid biosynthesis, resulted in significantly retarded CNS myelination; however, myelin appeared normal at 3 months of age...
May 2017: PLoS Biology
https://www.readbyqxmd.com/read/28542487/characterization-of-micrornas-expression-profiles-in-human-dental-derived-pluripotent-stem-cells
#15
Xiaobing Tan, Qingyuan Dai
Induced pluripotent stem cells (iPSCs) technology provides a powerful means to generate and regenerate unlimited pluripotent stem cells directly from body tissue cells. Stem cells from apical papilla (SCAP) and Dental pulp stem cells (DPSCs) are present in 'cell-rich zones' within the dental pulp region, which are capable of regenerating pulp and dentin tissues in vivo. In this study, we investigated the difference of miRNAs expression in SCAPs and DPSCs before and after the reprogramming. Using miRNA microarray, 134 and 265 differentially expressed miRNAs in DPSCs- and SCAP-iPSCs were up-regulated compared to these before reprogramming...
2017: PloS One
https://www.readbyqxmd.com/read/28542467/genetic-variations-in-sterol-regulatory-element-binding-protein-cleavage-activating-protein-scap-are-associated-with-blood-pressure-in-overweight-obese-chinese-children
#16
Yi-De Yang, Jie-Yun Song, Shuo Wang, Fang-Hong Liu, Yi-Ning Zhang, Xiao-Rui Shang, Hai-Jun Wang, Jun Ma
OBJECTIVE: Previous studies demonstrated a role of variations in sterol regulatory element binding protein (SREBP) cleavage-activating protein (SCAP) in obesity and blood lipids. But the associations between SCAP polymorphisms and blood pressure (BP) are not clear. This study aimed to investigate the relationship between genetic variations in SCAP and BP phenotypes in a Chinese pediatric population. METHODS: A case-control study on 702 high blood pressure (HBP) children and 1319 controls was conducted to explore the correlation between single nucleotide polymorphism markers (rs12487736 and rs12490383) of SCAP and BP phenotypes...
2017: PloS One
https://www.readbyqxmd.com/read/28521874/sirna-mediated-inhibition-of-srebp-cleavage-activating-protein-reduces-dyslipidemia-in-spontaneously-dysmetabolic-rhesus-monkeys
#17
Beth Ann Murphy, Marija Tadin-Strapps, Kristian Jensen, Robin Mogg, Andy Liaw, Kithsiri Herath, Gowri Bhat, David G McLaren, Stephen F Previs, Shirly Pinto
BACKGROUND: SREBP cleavage-activating protein (SCAP) is a cholesterol binding endoplasmic reticulum (ER) membrane protein that is required to activate SREBP transcription factors. SREBPs regulate genes involved in lipid biosynthesis. They also influence lipid clearance by modulating the expression of LDL receptor (LDLR) and proprotein convertase subtilisin/kexin type 9 (PCSK9) genes. Inhibiting SCAP decreases circulating PCSK9, triglycerides (TG), and LDL-cholesterol (LDL-C), both in vitro and in vivo...
June 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28502963/a-case-of-syringocystadenoma-papilliferum-with-tubular-papillary-adenoma-of-the-chest
#18
Keigo Ito, Shin-Ichi Ansai, Hidehisa Saeki
We report a case of syringocystadenoma papilliferum (SCAP) combined with tubular papillary adenoma (TPA) arising on the chest of a 45-year-old Japanese woman. Histopathological examination revealed the characteristic findings of SCAP in the superficial part of the lesion and those of TPA in the deeper part. We reviewed the English literature about this combination. SCAP and TPA have the same cellular components, but show differences of the general structure. The combination of these two neoplasms is more frequent than expected by most dermatopathologists or pathologists...
2017: Journal of Nippon Medical School, Nippon Ika Daigaku Zasshi
https://www.readbyqxmd.com/read/28502716/neuropeptides-predicted-from-the-transcriptome-analysis-of-the-gray-garden-slug-deroceras-reticulatum
#19
Seung-Joon Ahn, Ruth Martin, Sujaya Rao, Man-Yeon Choi
The gray garden slug, Deroceras reticulatum (Gastropoda: Pulmonata), is one of the most common terrestrial molluscs. Research for this slug has focused mainly on its ecology, biology, and management due to the severe damage it causes on a wide range of vegetables and field crops. However, little is known about neuropeptides and hormonal signalings. This study, therefore, aimed to establish the transcriptome of D. reticulatum and to identify a comprehensive repertoire of neuropeptides in this slug. Illumina high-throughput sequencing of the whole body transcriptome of D...
May 11, 2017: Peptides
https://www.readbyqxmd.com/read/28491104/effect-of-tetramethylpyrazine-on-atherosclerosis-and-scap-srebp-1c-signaling-pathway-in-apoe-mice-fed-with-a-high-fat-diet
#20
Ying Zhang, Pan Ren, Qunfu Kang, Weihong Liu, Sinai Li, Ping Li, Hongxu Liu, Juju Shang, Lei Zhang, Yanbing Gong, Mingxue Zhou
Lipid metabolism dysregulation plays a crucial role in the occurrence of atherosclerosis (As). SCAP/SREBP signaling is the main pathway for regulating lipid metabolism. Tetramethylpyrazine (TMP), a Traditional Chinese Medicine (TCM) for treating angina pectoris, has antiatherosclerotic effects and ameliorates blood lipids disturbance. However, its precise mechanism remains unclear. This study investigated the mechanism of TMP in ameliorating As in mice model. After six weeks of high-fat diet, 30 ApoE(-/-) mice were randomized (n = 10) and treated with Lipitor, TMP, or distilled water for six weeks...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
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