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https://www.readbyqxmd.com/read/28416302/effect-of-bacterial-biofilm-on-the-osteogenic-differentiation-of-stem-cells-of-apical-papilla
#1
Lakshmi Vishwanat, Rose Duong, Koyo Takimoto, Linda Phillips, Claudia O Espitia, Anibal Diogenes, Shivani B Ruparel, David Kolodrubetz, Nikita B Ruparel
INTRODUCTION: Although clinical success in regenerative endodontics is substantially high, histological success is limited to finding bone/cementum-like tissue instead of dentin within the canal space. The aims of this study were to investigate (1) the effect of bacterial biofilm on osteogenic gene expression in stem cells of the apical papilla (SCAP) and (2) the effect of bacterial antigens on the functional differentiation of SCAP into a mineralizing phenotype. METHODS: Using an ex vivo organotypic root canal model and an American Association of Endontists-recommended regenerative endodontic procedures, we evaluated SCAP differentiation in the presence and absence of an Enterococcus faecalis biofilm...
April 14, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28416161/cellular-senescence-a-translational-perspective
#2
REVIEW
James L Kirkland, Tamara Tchkonia
Cellular senescence entails essentially irreversible replicative arrest, apoptosis resistance, and frequently acquisition of a pro-inflammatory, tissue-destructive senescence-associated secretory phenotype (SASP). Senescent cells accumulate in various tissues with aging and at sites of pathogenesis in many chronic diseases and conditions. The SASP can contribute to senescence-related inflammation, metabolic dysregulation, stem cell dysfunction, aging phenotypes, chronic diseases, geriatric syndromes, and loss of resilience...
April 12, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28377508/cholesterol-induced-conformation-changes-in-the-sterol-sensing-domain-of-the-scap-protein-suggest-feedback-mechanism-to-control-cholesterol-synthesis
#3
Yansong Gao, Yulian Zhou, Joseph L Goldstein, Michael S Brown, Arun Radhakrishnan
Scap is a polytopic protein of endoplasmic reticulum (ER) membranes that transports sterol regulatory element-binding proteins to the Golgi complex for proteolytic activation. Cholesterol accumulation in ER membranes prevents Scap transport and decreases cholesterol synthesis. Previously, we provided evidence that cholesterol inhibition is initiated when cholesterol binds to loop 1 of Scap, which projects into the ER lumen. Within cells this binding causes loop 1 to dissociate from loop 7, another luminal Scap loop...
April 3, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28365680/pro-inflammatory-cytokine-tnf-%C3%AE-attenuates-bmp9-induced-osteo-odontoblastic-differentiation-of-the-stem-cells-of-dental-apical-papilla-scaps
#4
Feilong Wang, Ying Jiang, Xia Huang, Qiao Liu, Yan Zhang, Wenping Luo, Fugui Zhang, Pengfei Zhou, Juhong Lin, Hongmei Zhang
BACKGROUND/AIMS: Periapical periodontitis is a common oral disease caused by bacterial invasion of the tooth pulp, which usually leads to local release of pro-inflammatory cytokines and osteolytic lesion. This study is intended to examine the effect of TNF-α on BMP9-induced osteogenic differentiation of the stem cells of dental apical papilla (SCAPs). METHODS: Rat model of periapical periodontitis was established. TNF-α expression was assessed. Osteogenic markers and ectopic bone formation in iSCAPs were analyzed upon BMP9 and TNF-α treatment...
March 31, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28320569/a-new-method-to-predict-hospital-mortality-in-severe-community-acquired-pneumonia
#5
Xin Wang, Jianlong Jiao, Rongwei Wei, Yongli Feng, Xiuqin Ma, Yuan Li, Yue Du
BACKGROUND & AIMS: The aim of this study is to develop a new method that is able to accurately predict the 28day hospital mortality in patients with severe community acquired pneumonia (SCAP) at an early stage. METHODS: We selected 37,348 SCAP patients in ICU from 173 hospitals during 2011.1-2013.12. The predictive factors for 28day hospital mortality were evaluated retrospectively. All cases underwent intensive care, blood routine, blood biochemical tests and arterial blood gas analysis...
March 17, 2017: European Journal of Internal Medicine
https://www.readbyqxmd.com/read/28284693/the-effects-of-cell-death-inducing-dna-fragmentation-factor-%C3%AE-like-effector-c-cidec-on-milk-lipid-synthesis-in-mammary-glands-of-dairy-cows
#6
Yang Yang, Ye Lin, Xiaoyu Duan, He Lv, Weinan Xing, Qingzhang Li, Xuejun Gao, Xiaoming Hou
Adequate lipid synthesis by the mammary gland during lactation is essential for the survival of mammalian offspring. Cell death-inducing DNA fragmentation factor-α-like effector C (CIDEC) is a lipid droplet-associated protein and functions to promote lipid accumulation and inhibit lipolysis in mice and human adipocytes. However, the function of CIDEC in regulation of milk lipid synthesis in dairy cow mammary gland remains largely unknown. In this study, 6 multiparous Holstein cows (parity = 3) in early lactation were allocated to high-fat milk (milk yield 33...
May 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28244619/sfrp2-enhanced-the-adipogenic-and-neuronal-differentiation-potentials-of-stem-cells-from-apical-papilla
#7
Xiao Lin, Rui Dong, Shu Diao, Guoxia Yu, Liping Wang, Jun Li, Zhipeng Fan
Dental tissue-derived mesenchymal stem cells (MSCs) are easily obtained and considered as a favorable cell source for tissue engineering, but the regulation of direct differentiation is unknown, which restricts their application. The present study investigated the effect of SFRP2, a Wnt signaling modulator, on MSC differentiation using stem cells from apical papilla (SCAPs). The cells were cultured in specific inducing medium for adipogenic, neurogenic, or chondrogenic differentiation. Over-expression of SFRP2 via retroviral infection enhanced the adipogenic and neurogenic differentiation of SCAPs...
May 2017: Cell Biology International
https://www.readbyqxmd.com/read/28229417/a-simplified-and-systematic-method-to-isolate-culture-and-characterize-multiple-types-of-human-dental-stem-cells-from-a-single-tooth
#8
Mohammed Bakkar, Younan Liu, Dongdong Fang, Camille Stegen, Xinyun Su, Murali Ramamoorthi, Li-Chieh Lin, Takako Kawasaki, Nicholas Makhoul, Huan Pham, Yoshinori Sumita, Simon D Tran
This chapter describes a simplified method that allows the systematic isolation of multiple types of dental stem cells such as dental pulp stem cells (DPSC), periodontal ligament stem cells (PDLSC), and stem cells of the apical papilla (SCAP) from a single tooth. Of specific interest is the modified laboratory approach to harvest/retrieve the dental pulp tissue by minimizing trauma to DPSC by continuous irrigation, reduction of frictional heat from the bur rotation, and reduction of the bur contact time with the dentin...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28228593/er-protein-scap-inhibits-dengue-virus-ns2b3-protease-by-suppressing-its-k27-linked-polyubiquitylation
#9
Heng Liu, Lele Zhang, Jin Sun, Wei Chen, Senlin Li, Qiang Wang, Huansha Yu, Zanxian Xia, Xia Jin, Chen Wang
Dengue viruses (DENVs) are an emerging threat to global public health. The NS2B3 protease complex of DENV has recently been shown to cleave antiviral protein STING and thereby subverting the innate immune signaling to facilitate virus replication. Whether host cells have mechanism to counteract this virus-mediated immunosuppression is unclear. We discovered that the K27-linked poly-ubiquitination of NS3 protein facilitates its recruitment of NS2B and the formation of NS2B3, and consequently the enhanced cleavage of STING...
February 22, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28219188/-change-of-prognosis-and-pathogens-in-adult-patients-with-severe-community-acquired-pneumonia-during-different-periods
#10
L Zhang, P Ning, F Rong, Z C Gao
Objective: To compare the change of prognosis and pathogens of adult patients with severe community-acquired pneumonia (CAP) requiring admission to Respiratory Intensive Care Unit (RICU) during different period, and estimate the risk factors of severe CAP. Methods: The clinical data of 186 patients with severe CAP admission to RICU was collected and assessed the trend in outcomes of these patients during three time periods: Period Ⅰ (January 1 2000 to October 31 2006), Period Ⅱ (November 1 2006 to December 31 2010), and Period Ⅲ (January 1 2011 to December 31 2015), analyzed the risk factors by Cox Proportional Hazards regression, and compared the pathogens detection...
February 7, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28207658/association-between-paraspinal-muscle-morphology-clinical-symptoms-and-functional-status-in-patients-with-degenerative-cervical-myelopathy
#11
Maryse Fortin, Octavian Dobrescu, Matthew Courtemanche, Carolyn J Sparrey, Carlo Santaguida, Michael G Fehlings, Michael H Weber
STUDY DESIGN: A cross-sectional study. OBJECTIVE: The aim of this study was to assess fatty infiltration and asymmetry of the multifidus (MF), semispinalis cervicis (SCer), semispinalis capitis (SCap), and splenius capitis (SPL) muscles in patients with degenerative cervical myelopathy (DCM), and evaluate their correlations with clinical symptoms and functional scores. SUMMARY OF BACKGROUND DATA: Cervical muscle alterations have been reported in patients with chronic neck pain, but the assessment of cervical muscle morphology has been overlooked in patients with DCM...
February 15, 2017: Spine
https://www.readbyqxmd.com/read/28196330/decellularized-human-dental-pulp-as-a-scaffold-for-regenerative-endodontics
#12
J S Song, K Takimoto, M Jeon, J Vadakekalam, N B Ruparel, A Diogenes
Teeth undergo postnatal organogenesis relatively late in life and only complete full maturation a few years after the crown first erupts in the oral cavity. At this stage, development can be arrested if the tooth organ is damaged by either trauma or caries. Regenerative endodontic procedures (REPs) are a treatment alternative to conventional root canal treatment for immature teeth. These procedures rely on the transfer of apically positioned stem cells, including stem cells of the apical papilla (SCAP), into the root canal system...
February 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28190588/survival-of-the-apical-papilla-and-its-resident-stem-cells-in-a-case-of-advanced-pulpal-necrosis-and-apical-periodontitis
#13
Vanessa Chrepa, Brandon Pitcher, Michael A Henry, Anibal Diogenes
INTRODUCTION: Apical papilla represents a source of an enriched mesenchymal stem cell (MSC) population (stem cells of the apical papilla [SCAPs]) that modulates root development and may participate in regenerative endodontic procedures in immature teeth with pulp necrosis. The characteristics and phenotype of this tissue in the presence of inflammation are largely unknown. The purpose of this study was to characterize a human apical papilla sample that was isolated from an immature tooth with pulp necrosis and apical periodontitis...
April 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28168742/astrocyte-lipid-metabolism-is-critical-for-synapse-development-and-function-in-vivo
#14
Anne-Lieke F van Deijk, Nutabi Camargo, Jaap Timmerman, Tim Heistek, Jos F Brouwers, Floriana Mogavero, Huibert D Mansvelder, August B Smit, Mark H G Verheijen
The brain is considered to be autonomous in lipid synthesis with astrocytes producing lipids far more efficiently than neurons. Accordingly, it is generally assumed that astrocyte-derived lipids are taken up by neurons to support synapse formation and function. Initial confirmation of this assumption has been obtained in cell cultures, but whether astrocyte-derived lipids support synapses in vivo is not known. Here, we address this issue and determined the role of astrocyte lipid metabolism in hippocampal synapse formation and function in vivo...
February 7, 2017: Glia
https://www.readbyqxmd.com/read/28164259/five-year-mortality-in-patients-treated-for-severe-community-acquired-pneumonia-a-retrospective-study
#15
H Lenz, G O Norby, V Dahl, T E Ranheim, R E Haagensen
BACKGROUND: The mortality rate in patients with severe community-acquired pneumonia (SCAP) is high. We investigated the 5-year mortality rate and causes of death in a patient population treated for SCAP in our intensive care unit (ICU), and compared the mortality rate in patients with or without chronic obstructive pulmonary disease (COPD) as comorbidity. METHODS: This retrospective study, which covers a period of 10 years, included patients aged > 18 years admitted to our ICU with SCAP as primary diagnosis and in need of mechanical ventilation for more than 24 h...
April 2017: Acta Anaesthesiologica Scandinavica
https://www.readbyqxmd.com/read/28145066/analysis-of-gene-expression-profiles-between-apical-papilla-tissues-stem-cells-from-apical-papilla-and-cell-sheet-to-identify-the-key-modulators-in-mscs-niche
#16
Shu Diao, Xiao Lin, Liping Wang, Rui Dong, Juan Du, Dongmei Yang, Zhipeng Fan
OBJECTIVES: The microenvironmental niche plays the key role for maintaining the cell functions. The stem cells from apical papilla (SCAPs) are important for tooth development and regeneration. However, there is limited knowledge about the key factors in niche for maintaining the function of SCAPs. In this study, we analyse the gene expression profiles between apical papilla tissues, SCAPs and SCAPs cell sheet to identify the key genes in SCAPs niche. MATERIALS AND METHODS: Microarray assays and bioinformatic analysis were performed to screen the differential genes between apical papilla tissues and SCAPs, and SCAPs and SCAPs cell sheet...
February 1, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28132894/vitamin-d-metabolite-25-hydroxyvitamin-d-regulates-lipid-metabolism-by-inducing-degradation-of-srebp-scap
#17
Lisa Asano, Mizuki Watanabe, Yuta Ryoden, Kousuke Usuda, Takuya Yamaguchi, Bilon Khambu, Megumi Takashima, Shin-Ichi Sato, Juro Sakai, Kazuo Nagasawa, Motonari Uesugi
Sterol regulatory element-binding proteins (SREBPs) are transcription factors that control lipid homeostasis. SREBP activation is regulated by a negative feedback loop in which sterols bind to SREBP cleavage-activating protein (SCAP), an escort protein essential for SREBP activation, or to insulin-induced genes (Insigs) (endoplasmic reticulum [ER] anchor proteins), sequestering the SREBP-SCAP-Insig complex in the ER. We screened a chemical library of endogenous molecules and identified 25-hydroxyvitamin D (25OHD) as an inhibitor of SREBP activation...
January 23, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28123479/three-single-nucleotide-polymorphisms-associated-with-type-2-diabetes-mellitus-in-a-chinese-population
#18
Meijun Chen, Xuelong Zhang, Qingxiao Fang, Tongtong Wang, Tingting Li, Hong Qiao
An Indian study recently observed three new loci: rs9552911 in the SGCG, rs1593304 near PLXNA4 and rs4858889 in SCAP associated with type 2 diabetes mellitus (T2DM) in a south Asian population. The present study aimed to validate these findings in a Chinese population. We genotyped the above three single-nucleotide polymorphisms (SNPs), rs9552911, rs1593304, and rs4858889, in a group of 1,972 Chinese individuals, comprising of 966 type 2 diabetic patients and 976 controls. Anthropometric variables and biochemical traits were measured in all the participants...
January 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28122233/feedback-loop-regulation-of-scap-srebp-1-by-mir-29-modulates-egfr-signaling-driven-glioblastoma-growth
#19
Peng Ru, Peng Hu, Feng Geng, Xiaokui Mo, Chunming Cheng, Ji Young Yoo, Xiang Cheng, Xiaoning Wu, Jeffrey Yunhua Guo, Ichiro Nakano, Etienne Lefai, Balveen Kaur, Arnab Chakravarti, Deliang Guo
No abstract text is available yet for this article.
January 24, 2017: Cell Reports
https://www.readbyqxmd.com/read/28116873/the-scap-srebp-pathway-a-mediator-of-hepatic-steatosis
#20
REVIEW
Young Ah Moon
Nonalcoholic fatty liver disease (NAFLD) is strongly associated with insulin resistance, obesity, and dyslipidemia. NAFLD encompasses a wide range of states from the simple accumulation of triglycerides in the hepatocytes to serious states accompanied by inflammation and fibrosis in the liver. De novo lipogenesis has been shown to be a significant factor in the development of hepatic steatosis in insulin-resistant states. Sterol regulatory element binding protein-1c (SREBP-1c) is the main transcription factor that mediates the activation of lipogenesis, and SREBP cleavage activating protein (SCAP) is required for the activation of SREBPs...
March 2017: Endocrinology and Metabolism
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