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https://www.readbyqxmd.com/read/27918898/roles-of-l-type-calcium-channels-cav1-2-and-the-distal-c-terminus-dct-in-differentiation-and-mineralization-of-rat-dental-apical-papilla-stem-cells-rscaps
#1
Qianqian Gao, Jianping Ge, Yanqin Ju, Changsheng Li, Jian Gao, Meisheng Wu, Shouliang Zhao
OBJECTIVE: Voltage-gated inward Ca(2+) currents (ICa) are triggered by cell depolarization and commonly produce transient increases in the cytoplasmic free Ca(2+) concentration. The CaV1.2 distal C-terminus is susceptible to proteolytic cleavage, which yields a truncated CaV1.2 subunit and a cleaved C-terminal fragment (CCt or DCT). Stem cells from the apical papilla (SCAPs) has a capacity for differentiation into the odontoblastic-like cells in vitro and dentin forming in vivo, which makes SCAPs advantages in tissue engineering and regenerative endodontic...
November 4, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27911384/analysis-of-scap-n-glycosylation-and-trafficking-in-human-cells
#2
Chunming Cheng, Jeffrey Yunhua Guo, Feng Geng, Xiaoning Wu, Xiang Cheng, Qiyue Li, Deliang Guo
Elevated lipogenesis is a common characteristic of cancer and metabolic diseases. Sterol regulatory element-binding proteins (SREBPs), a family of membrane-bound transcription factors controlling the expression of genes important for the synthesis of cholesterol, fatty acids and phospholipids, are frequently upregulated in these diseases. In the process of SREBP nuclear translocation, SREBP-cleavage activating protein (SCAP) plays a central role in the trafficking of SREBP from the endoplasmic reticulum (ER) to the Golgi and in subsequent proteolysis activation...
November 8, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27885915/effect-of-an-snp-in-scap-gene-on-lipid-lowering-response-to-rosuvastatin-in-indian-patients-with-metabolic-syndrome
#3
Misbahuddin M Rafeeq, Farida Ahmad, Syed Z Rahman, Sheelu S Siddiqi, Shazi Shakeel
AIM: Statins treat dyslipidemia associated with metabolic syndrome. Genetic factors contribute to variable response. Sterol regulatory element-binding factors cleavage-activating protein (SCAP) pathway regulates lipid homeostasis, so effect of SNP in SCAP gene on rosuvastatin response was studied. MATERIALS & METHODS: Metabolic syndrome patients with low-density lipoprotein-cholesterol ≥130 mg/dl, were prescribed rosuvastatin 5 mg for 3 months. Lipids were measured initially and finally, and genotyping done...
November 25, 2016: Pharmacogenomics
https://www.readbyqxmd.com/read/27866836/physiological-suppression-of-lipotoxic-liver-damage%C3%A2-by-complementary-actions-of-hdac3-and%C3%A2-scap-srebp
#4
Romeo Papazyan, Zheng Sun, Yong Hoon Kim, Paul M Titchenell, David A Hill, Wenyun Lu, Manashree Damle, Min Wan, Yuxiang Zhang, Erika R Briggs, Joshua D Rabinowitz, Mitchell A Lazar
Liver fat accumulation precedes non-alcoholic steatohepatitis, an increasing cause of end-stage liver disease. Histone deacetylase 3 (HDAC3) is required for hepatic triglyceride homeostasis, and sterol regulatory element binding protein (SREBP) regulates the lipogenic response to feeding, but the crosstalk between these pathways is unknown. Here we show that inactivation of SREBP by hepatic deletion of SREBP cleavage activating protein (SCAP) abrogates the increase in lipogenesis caused by loss of HDAC3, but fatty acid oxidation remains defective...
November 10, 2016: Cell Metabolism
https://www.readbyqxmd.com/read/27833148/igf-1-igf-1r-hsa-let-7c-axis-regulates-the-committed-differentiation-of-stem-cells-from-apical-papilla
#5
Shu Ma, Genxia Liu, Lin Jin, Xiyao Pang, Yanqiu Wang, Zilu Wang, Yan Yu, Jinhua Yu
Insulin-like growth factor-1 (IGF-1) and its receptor IGF-1R play a paramount role in tooth/bone formation while hsa-let-7c actively participates in the osteogenic differentiation of mesenchymal stem cells. However, the interaction between IGF-1/IGF-1R and hsa-let-7c on the committed differentiation of stem cells from apical papilla (SCAPs) remains unclear. In this study, human SCAPs were isolated and treated with IGF-1 and hsa-let-7c over/low-expression viruses. The odonto/osteogenic differentiation of these stem cells and the involvement of mitogen-activated protein kinase (MAPK) pathway were subsequently investigated...
November 11, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27818690/stem-cells-of-dental-origin-current-research-trends-and-key-milestones-towards-clinical-application
#6
REVIEW
Athina Bakopoulou, Imad About
Dental Mesenchymal Stem Cells (MSCs), including Dental Pulp Stem Cells (DPSCs), Stem Cells from Human Exfoliated Deciduous teeth (SHED), and Stem Cells From Apical Papilla (SCAP), have been extensively studied using highly sophisticated in vitro and in vivo systems, yielding substantially improved understanding of their intriguing biological properties. Their capacity to reconstitute various dental and nondental tissues and the inherent angiogenic, neurogenic, and immunomodulatory properties of their secretome have been a subject of meticulous and costly research by various groups over the past decade...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27816546/anhydroicaritin-improves-diet-induced-obesity-and-hyperlipidemia-and-alleviates-insulin-resistance-by-suppressing-srebps-activation
#7
Zu-Guo Zheng, Ya-Ping Zhou, Xin Zhang, Pyone Myat Thu, Zhi-Shen Xie, Chong Lu, Tao Pang, Bin Xue, Da-Qian Xu, Yan Chen, Xiao-Wei Chen, Hui-Jun Li, Xiaojun Xu
SREBPs play important roles in the regulation of lipid metabolism, and are closely related to the occurrence and development of many metabolic diseases. Small molecular inhibitors of SERBPs are important tools in developing efficient treatment of metabolic diseases. However, there are no listing drug targeting SREBPs. Therefore, there is an urgent need to develop highly specific small molecules that inhibit SREBPs. In this study, using a hepatocyte-based high-throughput screening, we identified anhydroicaritin (AHI) as a novel inhibitor of SREBPs...
December 15, 2016: Biochemical Pharmacology
https://www.readbyqxmd.com/read/27811944/complex-structure-of-the-fission-yeast-srebp-scap-binding-domains-reveals-an-oligomeric-organization
#8
Xin Gong, Hongwu Qian, Wei Shao, Jingxian Li, Jianping Wu, Jun-Jie Liu, Wenqi Li, Hong-Wei Wang, Peter Espenshade, Nieng Yan
Sterol regulatory element-binding protein (SREBP) transcription factors are master regulators of cellular lipid homeostasis in mammals and oxygen-responsive regulators of hypoxic adaptation in fungi. SREBP C-terminus binds to the WD40 domain of SREBP cleavage-activating protein (SCAP), which confers sterol regulation by controlling the ER-to-Golgi transport of the SREBP-SCAP complex and access to the activating proteases in the Golgi. Here, we biochemically and structurally show that the carboxyl terminal domains (CTD) of Sre1 and Scp1, the fission yeast SREBP and SCAP, form a functional 4:4 oligomer and Sre1-CTD forms a dimer of dimers...
November 2016: Cell Research
https://www.readbyqxmd.com/read/27790258/evaluation-of-cytotoxic-effects-of-various-endodontic-irrigation-solutions-on-the-survival-of-stem-cell-of-human-apical-papilla
#9
Narges Farhad Mollashahi, Eshaghali Saberi, Hamed Karkehabadi
INTRODUCTION: Root canal disinfection is an important step in regenerative endodontic treatments. An ideal irrigating solution must have high antimicrobial activity and minimum cytotoxicity. This study sought to assess the effect of some irrigating solutions on stem cells from the human apical papilla (SCAP) after different periods of exposure. METHODS AND MATERIALS: Stem cells were isolated from immature, impacted mandibular third molars, transferred to 24-well plates, randomly divided into 6 experimental groups and exposed to BioPure MTAD Cleanser, QMix, 17% EDTA, 2% chlorhexidine (CHX), 5...
2016: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/27775170/engineering-three-dimensional-micro-nerve-tissue-using-postnatal-stem-cells-from-human-dental-apical-papilla
#10
Byung-Chul Kim, Sung-Min Jun, So Yeon Kim, Yong-Dae Kwon, Sung Chul Choe, Eun-Chul Kim, Jae-Hyung Lee, Jinseok Kim, Jun-Kyo Francis Suh, Yu-Shik Hwang
The in vitro generation of cell-based three dimensional (3D) nerve tissue is an attractive subject to improve graft survival and integration into host tissue for neural tissue regeneration or to model biological events in stem cell differentiation. Although 3D organotypic culture strategies are well established for 3D nerve tissue formation of pluripotent stem cells to study underlying biology in nerve development, cell-based nerve tissues have not been developed using human postnatal stem cells with therapeutic potential...
October 24, 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27761094/syringocystadenoma-papilliferum-of-the-scalp-arising-from-a-nevus-sebaceous
#11
Mathangi Chandramouli, Digvijay Sarma, Kapil Tejaswy, Gabriel Rodrigues
Syringocystadenoma papilliferum (SCAP) is a rare, benign tumour with varied presentations that arises from the apocrine glands and at times can lead to a diagnostic dilemma. A 53-year-old male presented with a 40-year history of a scalp nodule and a recent 2-year change in its character, and a sanguinopurulent discharge. Examination revealed a 4 cm × 2 cm firm nodule upon left side of the scalp vertex, actively discharging pus and blood. No regional lymph nodes were noticeably palpable. Incision biopsy performed elsewhere showed evidence of a squamous cell carcinoma-in-situ...
July 2016: Journal of Cutaneous and Aesthetic Surgery
https://www.readbyqxmd.com/read/27716996/the-transcription-factor-camp-response-element-binding-protein-enhances-the-odonto-osteogenic-differentiation-of-stem-cells-from-the-apical-papilla
#12
Shengzhe S, Yongna Z, Song L, Yan L, Jing Z
AIM: To investigate the role of cAMP response element-binding protein (CREB) in the regulation of odonto/osteogenic differentiation of stem cells from the apical papilla (SCAPs). METHODOLOGY: SCAPs were obtained from human impacted third molars (n=15). Isolated SCAPs were transfected with CREB overexpressing/silenced lentivirus. Transfected cells were stained with alizarin red to investigate mineralized nodule formation. The expression of the mineralization-related genes alkaline phosphatase (ALP), collagen type I (Col I), runt-related transcription factor 2 (RUNX2), osterix (OSX), and osteocalcin (OCN), was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR)...
October 7, 2016: International Endodontic Journal
https://www.readbyqxmd.com/read/27707816/dose-dependent-effects-of-sirna-mediated-inhibition-of-scap-on-pcsk9-ldlr-and-plasma-lipids-in-mouse-and-rhesus-monkey
#13
Kristian K Jensen, Marija Tadin-Strapps, Sheng-Ping Wang, James Hubert, Yanqing Kan, Yong Ma, David G McLaren, Stephen F Previs, Kithsiri B Herath, Ablatt Mahsut, Andy Liaw, Shubing Wang, Steven J Stout, CarolAnn Keohan, Gail Forrest, David Coelho, Satya Yendluri, Stephanie Williams, Martin Koser, Steven Bartz, Karen O Akinsanya, Shirly Pinto
SREBP cleavage-activating protein (SCAP) is a key protein in the regulation of lipid metabolism and a potential target for treatment of dyslipidemia. SCAP is required for activation of the transcription factors SREBP-1 and -2. SREBPs regulate the expression of genes involved in fatty acid and cholesterol biosynthesis, and LDL-C clearance through the regulation of LDL receptor (LDLR) and PCSK9 expression. To further test the potential of SCAP as a novel target for treatment of dyslipidemia, we used siRNAs to inhibit hepatic SCAP expression and assess the effect on PCSK9, LDLR, and lipids in mice and rhesus monkeys...
December 2016: Journal of Lipid Research
https://www.readbyqxmd.com/read/27705004/crp-il-6-il-10-single-nucleotide-polymorphisms-correlate-with-the-susceptibility-and-severity-of-community-acquired-pneumonia
#14
Shou-Chu Chou, How-Wen Ko, Ying-Chin Lin
OBJECTIVE: To explore the associations of single-nucleotide polymorphisms (SNPs) of the C-reactive protein (CRP), interleukin 6 (IL-6) and IL-10 genes with susceptibility and severity of community-acquired pneumonia (CAP). MATERIALS AND METHODS: Two hundred seventy-nine CAP patients were selected for the case group and 156 healthy individuals for the control group. Polymerase chain reaction-restriction fragment length polymorphism was performed for genotyping of CRP +1059G/C (rs1800947) and +1846C/T (rs1205), IL-6 -597G/A (rs1800797) and -572C/G (rs1800796), and IL-10 -592C/A (rs1800872) and -1082G/A (rs1800896)...
October 5, 2016: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/27687066/four-novel-polymorphisms-of-buffalo-insig2-gene-are-associated-with-milk-production-traits-in-chinese-buffaloes
#15
Tingxian Deng, Chunying Pang, Xiaoya Ma, Xingrong Lu, Anqin Duan, Peng Zhu, Xianwei Liang
Insulin-induced genes (INSIGs), including INSIG1 and INSIG2, are important mediators that play a pivotal role in the lipid metabolism and could cause the retention of the SCAP/SREBP complex. Therefore, the objective of this study is to detect the single nucleotide polymorphisms (SNPs) of buffalo INSIG2 gene and evaluate their associations with milk production traits in Chinese buffaloes. A total of four SNPs (g.621272A > G, g.621364A > C, g.632543G > A, and g.632684C > T) were identified using DNA pooled sequencing, and the SNP genotyping for the identified SNPs was performed by using Matrix-assisted laser desorption/ionization time of flight mass spectrometry method from 264 individuals...
September 27, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27671201/identification-of-the-c-terminal-domain-of-daxx-acts-as-a-potential-regulator-of-intracellular-cholesterol-synthesis-in-hepg2-cells
#16
Shaowei Sun, Juan Wen, Fei Qiu, Yufang Yin, Guina Xu, Tianping Li, Juan Nie, Guozuo Xiong, Caiping Zhang, Duangfang Liao, Jianxiong Chen, Qinhui Tuo
Daxx is a highly conserved nuclear transcriptional factor, which has been implicated in many nuclear processes including transcription and cell cycle regulation. Our previous study demonstrated Daxx also plays a role in regulation of intracellular cholesterol content. Daxx contains several domains that are essential for interaction with a growing number of proteins. To delineate the underlying mechanism of hypocholesterolemic activity of Daxx, we constructed a set of plasmids which can be used to overexpress different fragments of Daxx and transfected to HepG2 cells...
September 23, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27648074/the-regulatory-effects-of-long-noncoding-rna-ancr-on-dental-tissue-derived-stem-cells
#17
Qian Jia, Xiaolin Chen, Wenkai Jiang, Wei Wang, Bin Guo, Longxing Ni
Long noncoding RNAs (lncRNA) have been recognized as important regulators in diverse biological processes, such as transcriptional regulation, stem cell proliferation, and differentiation. Previous study has demonstrated that lncRNA-ANCR (antidifferentiation ncRNA) plays a key role in regulating the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). However, little is known about the role of ANCR in regulating other types of dental tissue-derived stem cells (DTSCs) behaviours (including proliferation and multiple-potential of differentiation)...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27641545/depletion-of-shank2-inhibited-the-osteo-dentinogenic-differentiation-potentials-of-stem-cells-from-apical-papilla
#18
Lijia Guo, Luyuan Jin, Juan Du, Chunmei Zhang, Zhipeng Fan, Songlin Wang
The aim of this study was to investigate the biological function of SHANK2 on the osteo/dentinogenic differentiation potentials of human stem cells from apical papilla (SCAPs). Real-time RT-PCR was used to detect the expression of SHANK2 in human mesenchymal stem cells (MSCs). Small hairpin RNA (shRNA) was used to knockdown the SHANK2 in SCAPs. The knockdown efficiency was determined by real-time RT-PCR and Western Blot. The in vitro osteo/dentinogenic differentiation potentials of SCAPs were investigated using ALP staining, ALP activity, alizarin red staining, quantitative calcium, the expression levels of DSPP, DMP1, RUNX2 and OSX...
September 19, 2016: Histology and Histopathology
https://www.readbyqxmd.com/read/27636889/extracellular-matrix-derived-hydrogels-for-dental-stem-cell-delivery
#19
Aiswarya Viswanath, Julie Vanacker, Loïc Germain, Julian G Leprince, Anibal Diogenes, Kevin M Shakesheff, Lisa J White, Anne des Rieux
Decellularized mammalian extracellular matrices (ECM) have been widely accepted as an ideal substrate for repair and remodelling of numerous tissues in clinical and pre-clinical studies. Recent studies have demonstrated the ability of ECM scaffolds derived from site-specific homologous tissues to direct cell differentiation. The present study investigated the suitability of hydrogels derived from different source tissues: bone, spinal cord and dentine, as suitable carriers to deliver human apical papilla derived mesenchymal stem cells (SCAP) for spinal cord regeneration...
January 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/27613575/comparison-of-stem-cell-behaviors-between-indigenous-high-and-low-cd24-percentage-expressing-cells-of-stem-cells-from-apical-papilla-scaps
#20
Panuroot Aguilar, Veera Lertchirakarn
CD24 was suggested as a marker to SCAPs and has been reported for a decade. CD24 has been shown to involve stem cell activities such as self-renewal, proliferation and differentiation. However, the percentage variations of CD24 positive cells were reported among the studies. It is possible that this variation may affect these SCAPs behaviors. In this study, the variation was confirmed. To elucidate the influence of CD24 positive cells quantity on SCAPs stem cell behaviors, the 3 cell lines with the most maximum and the least numbers of CD24 positive cells (High-CD24 and Low-CD24 group) were selected to study...
October 2016: Tissue & Cell
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