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https://www.readbyqxmd.com/read/28599789/microrna-126-participates-in-lipid-metabolism-in-mammary-epithelial-cells
#1
Meiqiang Chu, Yong Zhao, Yanni Feng, Hongfu Zhang, Jing Liu, Ming Cheng, Lan Li, Wei Shen, Hongfang Cao, Qiang Li, Lingjiang Min
Lipids are a major component of milk and are important for infant growth and development. MicroRNA-126 (miR-126) has previously been observed in mammary glands and adipocytes and is known to be involved in lipid metabolism during the process of atherosclerosis. However, it remains unknown whether miR-126 also participates in lipid metabolism in mammary luminal epithelial cells (MECs). In the current investigation, miR-126-3p inhibition stimulated lipid synthesis in MECs in part through increasing levels of the lipid synthesis enzymes FASN, ACSL1, and Insig1...
June 6, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28527797/mir-26b-promoter-analysis-reveals-regulatory-mechanisms-by-lipid-related-transcription-factors-in-goat-mammary-epithelial-cells
#2
Hui Wang, Jun Luo, Qiuya He, Dawei Yao, Jiao Wu, Juan J Loor
MicroRNA (miRNA) regulate protein abundance and control diverse aspects of cellular processes and biological functions associated with lipid metabolism. MiR-26b and its host gene CTDSP1 regulate triacylglycerol synthesis by synergistically suppressing the insulin-induced gene 1 (INSIG1); however, the direct regulators of miR-26b expression remain unknown. In the present study, we characterized the activity of a novel putative promoter region in miR-26b. Results revealed that promoter activity and miR-26b expression are dynamically regulated by different transcription factors including peroxisome proliferator-activated receptor gamma (PPARG), sterol regulatory element binding transcription factor 1 (SREBF1), and liver X receptor α (LXRα)...
July 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28235404/progesterone-signalling-in-broiler-skeletal-muscle-is-associated-with-divergent-feed-efficiency
#3
Walter Bottje, Byung-Whi Kong, Antonio Reverter, Ashley J Waardenberg, Kentu Lassiter, Nicholas J Hudson
BACKGROUND: We contrast the pectoralis muscle transcriptomes of broilers selected from within a single genetic line expressing divergent feed efficiency (FE) in an effort to improve our understanding of the mechanistic basis of FE. RESULTS: Application of a virtual muscle model to gene expression data pointed to a coordinated reduction in slow twitch muscle isoforms of the contractile apparatus (MYH15, TPM3, MYOZ2, TNNI1, MYL2, MYOM3, CSRP3, TNNT2), consistent with diminishment in associated slow machinery (myoglobin and phospholamban) in the high FE animals...
February 24, 2017: BMC Systems Biology
https://www.readbyqxmd.com/read/28207535/an-investigation-of-obesity-susceptibility-genes-in-northern-han-chinese-by-targeted-resequencing
#4
Yili Wu, Weijing Wang, Wenjie Jiang, Jie Yao, Dongfeng Zhang
Our earlier genome-wide linkage study of body mass index (BMI) showed strong signals from 7q36.3 and 8q21.13. This case-control study set to investigate 2 genomic regions which may harbor variants contributed to development of obesity.We employed targeted resequencing technology to detect single nucleotide polymorphisms (SNPs) in 7q36.3 and 8q21.13 from 16 individuals with obesity. These were compared with 504 East Asians in the 1000 Genomes Project as a reference panel. Linkage disequilibrium (LD) block analysis was performed for the significant SNPs located near the same gene...
February 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28146316/mir-92a-enhances-recombinant-protein-productivity-in-cho-cells-by-increasing-intracellular-cholesterol-levels
#5
Wan Ping Loh, Yuansheng Yang, Kong Peng Lam
MicroRNAs (miRNAs) have emerged as promising targets for engineering of CHO cell factories to enhance recombinant protein productivity. Manipulation of miRNA levels in CHO cells have been shown to improve product yield by increasing proliferation and specific productivity (qP), resisting apoptosis and enhancing oxidative metabolism. The authors previously demonstrated that over-expressing miR-92a results in increases in qP and titer of CHO-IgG cells. However, the mechanisms by which miR-92a enhances qP in CHO cells are still uninvestigated...
April 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/27918728/cholesterol-metabolism-in-rabbit-blastocysts-under-maternal-diabetes
#6
S Mareike Pendzialek, Maria Schindler, Torsten Plösch, Jacqueline Gürke, Elisa Haucke, Stefanie Hecht, Bernd Fischer, Anne Navarrete Santos
In the rabbit reproductive model, maternal experimentally induced insulin-dependent diabetes mellitus (expIDD) leads to accumulation of lipid droplets in blastocysts. Cholesterol metabolism is a likely candidate to explain such metabolic changes. Therefore, in the present study we analysed maternal and embryonic cholesterol concentrations and expression of related genes in vivo (diabetic model) and in vitro (embryo culture in hyperglycaemic medium). In pregnant expIDD rabbits, the serum composition of lipoprotein subfractions was changed, with a decrease in high-density lipoprotein cholesterol and an increase in very low-density lipoprotein cholesterol; in uterine fluid, total cholesterol concentrations were elevated...
December 6, 2016: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/27699590/high-fructose-diet-is-as-detrimental-as-high-fat-diet-in-the-induction-of-insulin-resistance-and-diabetes-mediated-by-hepatic-pancreatic-endoplasmic-reticulum-er-stress
#7
M Balakumar, L Raji, D Prabhu, C Sathishkumar, P Prabu, V Mohan, M Balasubramanyam
In the context of high human consumption of fructose diets, there is an imperative need to understand how dietary fructose intake influence cellular and molecular mechanisms and thereby affect β-cell dysfunction and insulin resistance. While evidence exists for a relationship between high-fat-induced insulin resistance and metabolic disorders, there is lack of studies in relation to high-fructose diet. Therefore, we attempted to study the effect of different diets viz., high-fat diet (HFD), high-fructose diet (HFS), and a combination (HFS + HFD) diet on glucose homeostasis and insulin sensitivity in male Wistar rats compared to control animals fed with normal pellet diet...
December 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/27687066/four-novel-polymorphisms-of-buffalo-insig2-gene-are-associated-with-milk-production-traits-in-chinese-buffaloes
#8
Tingxian Deng, Chunying Pang, Xiaoya Ma, Xingrong Lu, Anqin Duan, Peng Zhu, Xianwei Liang
Insulin-induced genes (INSIGs), including INSIG1 and INSIG2, are important mediators that play a pivotal role in the lipid metabolism and could cause the retention of the SCAP/SREBP complex. Therefore, the objective of this study is to detect the single nucleotide polymorphisms (SNPs) of buffalo INSIG2 gene and evaluate their associations with milk production traits in Chinese buffaloes. A total of four SNPs (g.621272A > G, g.621364A > C, g.632543G > A, and g.632684C > T) were identified using DNA pooled sequencing, and the SNP genotyping for the identified SNPs was performed by using Matrix-assisted laser desorption/ionization time of flight mass spectrometry method from 264 individuals...
September 27, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27640649/the-mir-33-gene-is-identified-in-a-marine-teleost-a-potential-role-in-regulation-of-lc-pufa-biosynthesis-in-siganus-canaliculatus
#9
Qinghao Zhang, Cuihong You, Shuqi Wang, Yewei Dong, Óscar Monroig, Douglas R Tocher, Yuanyou Li
As the first marine teleost demonstrated to have the ability to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFA) from C18 PUFA precursors, rabbitfish Siganus canaliculatus provides a good model for studying the regulatory mechanisms of LC-PUFA biosynthesis in teleosts. Here the potential roles of miR-33 in such regulation were investigated. The miR-33 gene was identified within intron 16 of the gene encoding sterol regulatory element-binding protein 1 (Srebp1), an activator of LC-PUFA biosynthesis...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27497905/isolation-of-rna-from-milk-somatic-cells-as-an-alternative-to-biopsies-of-mammary-tissue-for-nutrigenomic-studies-in-dairy-ewes
#10
P G Toral, G Hervás, A Suárez-Vega, J J Arranz, P Frutos
Nutrigenomic studies of mammary lipogenesis in ruminants often rely on the use of mammary tissue (MT) collected either by biopsy or at slaughter. However, isolating RNA from milk would be a useful and cost-effective technique that may avoid distress to the animal and facilitate the collection of samples in time series experiments. This assay was therefore conducted to test the hypothesis that RNA extracted from milk somatic cells (MSC) in dairy sheep would be a feasible alternative to the performance of MT biopsies for nutrigenomic analyses...
October 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27493725/essential-amino-acid-ratios-and-mtor-affect-lipogenic-gene-networks-and-mirna-expression-in-bovine-mammary-epithelial-cells
#11
Shanshan Li, Afshin Hosseini, Marina Danes, Carolina Jacometo, Jianxin Liu, Juan J Loor
BACKGROUND: The objective of this study was to study how changing the ratio of Lys to Thr, Lys to His, and Lys to Val affects the expression of lipogenic genes and microRNA (miRNA) in bovine mammary epithelial cells. RESULTS: Triplicate cultures with the respective "optimal" amino acid (AA) ratio (OPAA = Lys:Met 2.9:1; Thr:Phe 1.05:1; Lys:Thr 1.8:1; Lys:His 2.38:1; Lys:Val 1.23:1) plus rapamycin (OPAARMC; positive control), OPAA, Lys:Thr 2.1:1 (LT2.1), Lys:Thr 1...
2016: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/27477848/genome-wide-transcriptome-induced-by-nickel-in-human-monocytes
#12
Lina Gölz, Benedikt C Buerfent, Andrea Hofmann, Heiko Rühl, Nadine Fricker, Wiebke Stamminger, Johannes Oldenburg, James Deschner, Achim Hoerauf, Markus M Nöthen, Johannes Schumacher, Marc P Hübner, Andreas Jäger
UNLABELLED: Nickel-containing alloys are frequently used in the biomedical field, although, owing to corrosive processes metal ion leaching is inevitable. Due to nickel ion (Ni(2+)) leaching several adverse effects are described in the literature. However, only a few studies evaluated the genetic profile of Ni(2+) in human cells which is of great importance since nickel-induced effects differ between humans and mice as a result of species-specific receptor variability. Thus, we investigated gene expression induced by Ni(2+)in human monocytes using a transcriptome-wide approach determining new target genes implicated in nickel-induced pathologies...
October 1, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27474983/fish-oil-induced-milk-fat-depression-and-associated-downregulation-of-mammary-lipogenic-genes-in-dairy-ewes
#13
RANDOMIZED CONTROLLED TRIAL
D Carreño, G Hervás, P G Toral, T Castro-Carrera, P Frutos
Several studies in dairy cows have shown a relationship between milk fat depression (MFD) and alterations caused in lipogenic gene expression by dietary nutrients. However, information on small ruminants is not only scarce but also inconsistent. Therefore, this experiment was conducted in dairy ewes to study the effect of a diet known to induce MFD on milk fatty acid (FA) composition and mRNA abundance of key candidate genes involved in mammary lipogenesis. Twelve lactating Assaf ewes (on average 63d in milk) were randomly assigned to 2 treatments consisting of a total mixed ration based on alfalfa hay and concentrates (50:50), supplemented with 0 (control) or 17g of fish oil/kg of diet dry matter (FO)...
October 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27448180/mir-145-regulates-lipogenesis-in-goat-mammary-cells-via-targeting-insig1-and-epigenetic-regulation-of-lipid-related-genes
#14
Hui Wang, Huaiping Shi, Jun Luo, Yongqing Yi, Dawei Yao, Xueying Zhang, Gongzhen Ma, Juan J Loor
MicroRNAs (miRNAs) are noncoding RNA molecules that regulate gene expression at the post-transcriptional level to cause translational repression or degradation of targets. The profiles of miRNAs across stages of lactation in small ruminant species such as dairy goats is unknown. A small RNA library was constructed using tissue samples from mammary gland of Saanen dairy goats harvested at mid-lactation followed by sequencing via Solexa technology. A total of 796 conserved miRNAs, 263 new miRNAs, and 821 pre-miRNAs were uncovered...
May 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27198006/statin-induced-expression-change-of-insig1-in-lymphoblastoid-cell-lines-correlates-with-plasma-triglyceride-statin-response-in-a-sex-specific-manner
#15
E Theusch, K Kim, K Stevens, J D Smith, Y-D I Chen, J I Rotter, D A Nickerson, M W Medina
No abstract text is available yet for this article.
2016: Pharmacogenomics Journal
https://www.readbyqxmd.com/read/27173213/amfr-gene-silencing-inhibits-the-differentiation-of-porcine-preadipocytes
#16
C Z Chen, Y N Zhu, M L Chai, L S Dai, Y Gao, H Jiang, L J Zhang, Y Ding, S Y Liu, Q Y Li, W F Lu, J B Zhang
Our study clarifies the role of the autocrine motility factor receptor (AMFR) gene in porcine preadipocyte differentiation. AMFR-siRNA was transfected into porcine preadipocytes and the preadipocytes were induced to differentiation. Subsequently, qRT-PCR was conducted to examine changes in mRNA expression of a series of genes in porcine preadipocytes, including AMFR, sterol-regulatory element-binding protein-1a (SREBP1a), SREBP2, insulin-induced gene 1 (Insig1), and Insig2. Expression changes in the mRNA of genes regulating adipocyte differentiation were also analyzed using qRT-PCR, including peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), and Kruppel-like factor 2 (KLF2)...
April 7, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27023521/knock-down-of-endogenous-bornavirus-like-nucleoprotein-1-inhibits-cell-growth-and-induces-apoptosis-in-human-oligodendroglia-cells
#17
Peng He, Lin Sun, Dan Zhu, Hong Zhang, Liang Zhang, Yujie Guo, Siwen Liu, Jingjing Zhou, Xiaoyan Xu, Peng Xie
Endogenous bornavirus-like nucleoprotein elements (EBLNs) have been discovered in the genomes of various animals including humans, whose functions have been seldom studied. To explore the biological functions of human EBLNs, we constructed a lentiviral vector expressing a short-hairpin RNA against human EBLN1, which successfully inhibited EBLN1 expression by above 80% in infected human oligodendroglia cells (OL cells). We found that EBLN1 silencing suppressed cell proliferation, induced G2/M phase arrest, and promoted apoptosis in OL cells...
March 24, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27008801/cyclosporin-a-affects-the-proliferation-process-in-normal-human-dermal-fibroblasts
#18
Grazyna Janikowska, Tomasz Janikowsk, Alina Pyka, Adam Wilczok, Urszula Mazurek
Cyclosporin A is an immunosuppressant drug that is used not only in solid transplant rejection, but also in moderate and severe forms of psoriasis, pyoderma, lupus or arthritis. Serious side effects of the drug such as skin cancer or gingival hyperplasia probably start with the latent proliferation process. Little is known about the influence of cyclosporin A on molecular signaling in epidermal tissue. Thus, the aim of this study was to estimate the influence of cyclosporin A on the process of proliferation in normal human dermal fibroblasts...
January 2016: Acta Poloniae Pharmaceutica
https://www.readbyqxmd.com/read/27002347/microrna-26a-b-and-their-host-genes-synergistically-regulate-triacylglycerol-synthesis-by-targeting-the-insig1-gene
#19
Hui Wang, Jun Luo, Tianying Zhang, Huibin Tian, Yue Ma, Huifen Xu, Dawei Yao, Juan J Loor
The microRNA-26 (miR-26) family is known to control adipogenesis in non-ruminants. The genomic loci of miR-26a and miR-26b have been localized in the introns of genes encoding for the proteins of the C-terminal domain RNA polymerase II polypeptide A small phosphatase (CTDSP) family. Insulin-induced gene 1 (INSIG1) encodes a protein with a key role in the regulation of lipogenesis in rodent liver. In the present study, we investigated the synergistic function of the miR-26 family and their host genes in goat mammary epithelial cells (GMEC)...
May 3, 2016: RNA Biology
https://www.readbyqxmd.com/read/26945853/rna-binding-protein-hud-reduces-triglyceride-production-in-pancreatic-%C3%AE-cells-by-enhancing-the-expression-of-insulin-induced-gene-1
#20
Chongtae Kim, Heejin Lee, Hoin Kang, Jung Jae Shin, Hyosun Tak, Wook Kim, Myriam Gorospe, Eun Kyung Lee
Although triglyceride (TG) accumulation in the pancreas leads to β-cell dysfunction and raises the chance to develop metabolic disorders such as type 2 diabetes (T2DM), the molecular mechanisms whereby intracellular TG levels are regulated in pancreatic β cells have not been fully elucidated. Here, we present evidence that the RNA-binding protein HuD regulates TG production in pancreatic β cells. Mouse insulinoma βTC6 cells stably expressing a small hairpin RNA targeting HuD (shHuD) (βTC6-shHuD) contained higher TG levels compared to control cells...
April 2016: Biochimica et Biophysica Acta
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