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Limbal deficiency

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https://www.readbyqxmd.com/read/29283122/results-of-simple-limbal-epithelial-transplantation-in-unilateral-ocular-surface-burn
#1
Nidhi Gupta, Jagdish Joshi, Javed Hussain Farooqui, Umang Mathur
PURPOSE: This study aimed to report the long-term outcomes of autologous Simple Limbal Epithelial Transplantation (SLET) performed for unilateral limbal stem cell deficiency (LSCD) following chemical burn at a tertiary eye center in North India. METHODS: This was a single-center prospective interventional case series of patients who developed unilateral LSCD after suffering from ocular surface burns and who underwent SLET between October 2012 and May 2016 with a follow-up period of at least 6 months...
January 2018: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29238604/novel-pax6-mutation-reported-in-an-aniridia-patient
#2
Andrew Winegarner, Yoshinori Oie, Satoshi Kawasaki, Nozomi Nishida, Kohji Nishida
An aniridia patient was found to have a novel PAX6 mutation. A genetic duplication within PAX6, which caused a frameshift mutation, ultimately created a nonsense stop codon and premature truncation of the protein. Consequently, the patient presented with a clouded cornea as a result of partial limbal stem cell deficiency, foveal hypoplasia, nystagmus and a pale, cupped optic disc caused by glaucoma.
2017: Human Genome Variation
https://www.readbyqxmd.com/read/29230251/establishment-of-novel-limbus-derived-highly-proliferative-abcg2-abcb5-limbal-epithelial-stem-cell-cultures
#3
Eung Kweon Kim, Ga-Hyun Lee, Boram Lee, Yong-Sun Maeng
Homeostasis and regeneration of corneal epithelia are sustained by limbal epithelial stem cells (LESCs); thus, an LESC deficiency is a major cause of blindness worldwide. Despite the generally promising results of cultivated LESC transplantation, it has been limited by variations in long-term success rates, the use of xenogeneic and undefined culture components, and a scarcity of donor tissues. In this study, we identified the culture conditions required to expand LESCs in vitro and established human limbus-derived highly proliferative ABCG2+/ABCB5+ double-positive LESCs...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29226476/differences-in-the-activity-of-endogenous-bmp-signalling-impact-on-the-ability-of-induced-pluripotent-stem-cells-to-differentiate-to-corneal-epithelial-like-cells
#4
Taty Anna Kamarudin, Sanja Bojic, Joseph Collin, Min Yu, Sameer Alharthi, Harley Buck, Alex Shortt, Lyle Armstrong, Francisco C Figueiredo, Majlinda Lako
Cornea is a clear outermost layer of the eye which enables transmission of light onto the retina. The transparent corneal epithelium is regenerated by limbal stem cells (LSCs), whose loss/dysfunction results in limbal stem cell deficiency (LSCD). Ex vivo expansion of autologous LSCs obtained from patient's healthy eye followed by transplantation onto the LSCs damaged/deficient eye, has provided a successful treatment for unilateral LSCD. This however is not applicable to patient with total bilateral LSCD, where LSCs are lost/damaged from both eyes...
December 11, 2017: Stem Cells
https://www.readbyqxmd.com/read/29217022/focal-limbal-stem-cell-deficiency-associated-with-soft-contact-lens-wear
#5
Karolien Termote, Steven Schendel, Gregory Moloney, Simon P Holland, Alex P Lange
OBJECTIVE: The aim of this study is to summarize the clinical characteristics of patients with contact lens-associated focal limbal stem cell deficiency (FLSCD) from a tertiary corneal referral centre. DESIGN: Retrospective, observational case series in a tertiary care centre. METHODS: Patients with contact lens-associated FLCSD were identified in our database. Clinical data were retrieved by chart review. A questionnaire asking for contact lens brand, type, cleaning solution, and duration of contact lens wear was sent to the patients with telephone follow-up...
December 2017: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
https://www.readbyqxmd.com/read/29210839/cataract-surgery-in-ocular-surface-diseases-clinical-challenges-and-outcomes
#6
REVIEW
Virender S Sangwan, Swati Gupta, Shilpa Das
PURPOSE OF REVIEW: Cataracts are a significant cause of blindness and visual impairment worldwide. With the recent advances in cataract surgery techniques, outcomes have improved significantly in uncomplicated cases. This article discusses the challenges and outcomes of cataract surgery in complex cases targeting eyes with ocular surface diseases like Stevens-Johnson syndrome, ocular cicatricial pemphigoid, Mooren's ulcer, vernal keratoconjunctivitis and limbal stem cell deficiency. RECENT FINDINGS: Ocular surface diseases are commonly associated with corneal scarring and vascularization, conjunctival inflammation, symblepharon and forniceal shortening...
January 2018: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/29198663/determination-of-the-culture-time-point-to-induce-corneal-epithelial-differentiation-in-induced-pluripotent-stem-cells
#7
R A Martínez García de la Torre, N Nieto-Nicolau, A Morales-Pastor, R P Casaroli-Marano
BACKGROUND: Limbal stem cells (LSC) are progenitor cells in the ocular surface that renew the corneal epithelium. Limbal stem cell deficiency usually induces blindness through the loss of corneal transparency, and bilateral cases do not an accurate treatment because of the lack of an autologous source of stem cells. METHODS: Induced pluripotent stem cells (iPSC) are promising for use in cell therapy because of their autologous origin and the capability to differentiate into corneal epithelial cells...
December 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/29172876/limbal-stem-cell-transplantation-and-complications
#8
Jia Yin, Ula Jurkunas
Corneal epithelial stem cells are adult somatic stem cells located at the limbus and represent the ultimate source of transparent corneal epithelium. When these limbal stem cells become dysfunctional or deficient, limbal stem cell deficiency (LSCD) develops. LSCD is a major cause of corneal scarring and is particularly prevalent in chemical and thermal burns of the ocular surface. LSCD leads to conjunctivalization of the corneal surface, neovascularization, recurrent or persistent epithelial defects, ocular surface inflammation, and scarring that, in turn, lead to decreased vision, pain, and impaired quality of life...
November 27, 2017: Seminars in Ophthalmology
https://www.readbyqxmd.com/read/29141906/keratolimbal-allograft-for-limbal-stem-cell-deficiency-after-severe-corneal-chemical-injury-a-systematic-review
#9
Swapna S Shanbhag, Hajirah N Saeed, Eleftherios I Paschalis, James Chodosh
PURPOSE: To review the published literature on outcomes of keratolimbal allograft (KLAL) for the surgical treatment of limbal stem cell deficiency (LSCD) and corneal blindness after severe corneal chemical injury. METHODS: Literature searches were conducted in the following electronic databases: MEDLINE, EMBASE, Science Citation Index, CINAHL, LILACS and the Cochrane Library. Standard systematic review methodology was applied. The main outcome measure was the proportion of eyes with best-corrected visual acuity (BCVA) ≥20/200 at last follow-up...
November 15, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29133636/diagnosis-of-limbal-stem-cell-deficiency-based-on-corneal-epithelial-thickness-measured-on-anterior-segment-optical-coherence-tomography
#10
COMPARATIVE STUDY
Amit Mehtani, Mahesh Chandra Agarwal, Sushant Sharma, Santosh Chaudhary
PURPOSE: The purpose of this study is to investigate the epithelial thickness in the cornea and limbus in limbal stem cell deficiency (LSCD) using anterior segment optical coherence tomography (AS-OCT). METHODS: This was a cross-sectional, comparative study. OCT images of 30 eyes of 19 patients with LSCD collected by AS-OCT were scanned. Corneal epithelial thickness was recorded at the central cornea and the superior, nasal, inferior, and temporal limbus. Measurment of the same region of 30 normal eyes served as control...
November 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29133179/stage-related-central-corneal-epithelial-transformation-in-congenital-aniridia-associated-keratopathy
#11
Neil Lagali, Bogumil Wowra, Dariusz Dobrowolski, Tor Paaske Utheim, Per Fagerholm, Edward Wylegala
PURPOSE: To relate central corneal epithelial phenotype to degree of keratopathy in a limbal stem cell deficient population. METHODS: 37 patients (67 eyes) with aniridia-associated keratopathy (AAK) underwent corneal examination including slit lamp biomicroscopy to determine the Grade of AAK, Cochet-Bonnet esthesiometry, and in vivo confocal microscopy (IVCM) to assess morphology of the central corneal epithelium and subepithelial region. RESULTS: AAK Grade ranged from 1 (limbal involvement only) to 4 (total conjunctivalisation), with progression from Grade 1 occurring after the age of 20...
November 10, 2017: Ocular Surface
https://www.readbyqxmd.com/read/29127331/divergent-effects-of-wnt-%C3%AE-catenin-signaling-modifiers-on-the-preservation-of-human-limbal-epithelial-progenitors-according-to-culture-condition
#12
Hyun Jung Lee, J Mario Wolosin, So-Hyang Chung
Wnt signaling plays an important role in the regulation of self-renewal in stem cells. Here we investigated the effect of CHIR99021, the primary transducer of the Wnt signaling canonical pathway, and IWP2, a wide action Wnt signal blocker, on the growth and differentiation of the limbal epithelial progenitor cells when these cells are cultured in two different, common culture approaches, outgrowth from limbal biopsy explants and isolated cell seeded in low calcium medium. Consistent with their expected effects, irrespective of the culture system, IWP2 decreased total β-catenin while CHIR99021 increased it in nuclear localization...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29116027/poly-ethylene-glycol-modified-silk-fibroin-membrane-as-a-carrier-for-limbal-epithelial-stem-cell-transplantation-in-a-rabbit-lscd-model
#13
Yijian Li, Yuli Yang, Lei Yang, Yuxiao Zeng, Xiaowei Gao, Haiwei Xu
BACKGROUND: Limbal epithelial stem cells (LESCs) play important roles in corneal epithelial homeostasis and regeneration, and damage to the limbus will lead to limbal stem cell deficiency (LSCD), with conjunctivalization and even visual impairment. Cultured LESCs have been used for ocular surface reconstruction, and silk fibroin (SF) membranes have shown potential as a substrate for LESC cultivation. Both culture methods and the carriers of LESCs affect outcomes following LESC transplantation...
November 7, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29113917/the-diagnosis-of-limbal-stem-cell-deficiency
#14
REVIEW
Qihua Le, Jianjiang Xu, Sophie X Deng
Limbal stem cells (LSCs) maintain the normal homeostasis and wound healing of corneal epithelium. Limbal stem cell deficiency (LSCD) is a pathologic condition that results from the dysfunction and/or an insufficient quantity of LSCs. The diagnosis of LSCD has been made mainly based on medical history and clinical signs, which often are not specific to LSCD. Methods to stage the severity of LSCD have been lacking. With the application of newly developed ocular imaging modalities and molecular methods as diagnostic tools, standardized quantitative criteria for the staging of LSCD can be established...
November 4, 2017: Ocular Surface
https://www.readbyqxmd.com/read/29109898/limbal-stem-cell-deficiency-and-treatment-with-stem-cell-transplantation
#15
REVIEW
Özlem Barut Selver, Ayşe Yağcı, Sait Eğrilmez, Mehmet Gürdal, Melis Palamar, Türker Çavuşoğlu, Utku Ateş, Ali Veral, Çağrı Güven, Jose Mario Wolosin
The cornea is the outermost tissue of the eye and it must be transparent for the maintenance of good visual function. The superficial epithelium of the cornea, which is renewed continuously by corneal stem cells, plays a critical role in the permanence of this transparency. These stem cells are localized at the cornea-conjunctival transition zone, referred to as the limbus. When this zone is affected/destroyed, limbal stem cell deficiency ensues. Loss of limbal stem cell function allows colonization of the corneal surface by conjunctival epithelium...
October 2017: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/29107055/native-and-synthetic-scaffolds-for-limbal-epithelial-stem-cell-transplantation
#16
REVIEW
Kim N Nguyen, Samantha Bobba, Alexander Richardson, Mijeong Park, Stephanie L Watson, Denis Wakefield, Nick Di Girolamo
Limbal stem cell deficiency (LSCD) is a complex blinding disease of the cornea, which cannot be treated with conventional corneal transplants. Instead, a stem cell (SC) graft is required to replenish the limbal epithelial stem cell (LESC) reservoir, which is ultimately responsible for regenerating the corneal epithelium. Current therapies utilize limbal tissue biopsies that harbor LESCs as well as tissue culture expanded cells. Typically, this tissue is placed on a scaffold that supports the formation of corneal epithelial cell sheets, which are then transferred to diseased eyes...
November 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29105366/limbal-stem-cells-identity-developmental-origin-and-therapeutic-potential
#17
REVIEW
Gabriel Gonzalez, Yuzuru Sasamoto, Bruce R Ksander, Markus H Frank, Natasha Y Frank
The cornea is our window to the world and our vision is critically dependent on corneal clarity and integrity. Its epithelium represents one of the most rapidly regenerating mammalian tissues, undergoing full-turnover over the course of approximately 1-2 weeks. This robust and efficient regenerative capacity is dependent on the function of stem cells residing in the limbus, a structure marking the border between the cornea and the conjunctiva. Limbal stem cells (LSC) represent a quiescent cell population with proliferative capacity residing in the basal epithelial layer of the limbus within a cellular niche...
November 3, 2017: Wiley Interdisciplinary Reviews. Developmental Biology
https://www.readbyqxmd.com/read/29104070/the-role-of-amniotic-membrane-extract-eye-drop-ameed-in-in-vivo-cultivation-of-limbal-stem-cells
#18
Alireza Baradaran-Rafii, Niloufar Shayan Asl, Marzieh Ebrahimi, Sayena Jabbehdari, Shahram Bamdad, Danial Roshandel, Medi Eslani, Maryam Momeni
BACKGROUND: Limbal stem cell transplantation (LSCT) is the definitive treatment for total limbal stem cell deficiency (LSCD). This study evaluates the anatomical and visual outcomes of a surgical technique supplemented by amniotic membrane extract eye drop (AMEED) for in vivo cultivation of limbal stem cells (LSCs). METHODS: One small limbal block (2 × 1 mm) harvested from the contralateral healthy eye was transferred to the diseased eye, which had been already covered by cryopreserved amniotic membrane (N = 20)...
November 2, 2017: Ocular Surface
https://www.readbyqxmd.com/read/29099734/combined-conjunctival-limbal-autografts-and-living-related-conjunctival-limbal-allografts-for-severe-unilateral-ocular-surface-failure
#19
Albert Y Cheung, Enrica Sarnicola, Amit Govil, Edward J Holland
PURPOSE: To describe the technique and present 2 cases of a combined conjunctival limbal autograft (CLAU) and living-related conjunctival limbal allograft (lr-CLAL) procedure for treatment of severe unilateral ocular surface failure. METHODS: Interventional case series of 2 eyes of 2 patients sustaining severe thermal/chemical injuries from firework explosions. They both underwent the combined CLAU/lr-CLAL procedure followed by penetrating keratoplasty. Systemic immunosuppression consisted of oral tacrolimus and mycophenolate mofetil...
December 2017: Cornea
https://www.readbyqxmd.com/read/29099638/type-1-boston-keratoprosthesis-for-limbal-stem-cell-deficiency-in-epidermolysis-bullosa
#20
N Geetha Sravani, Ashik Mohamed, Virender S Sangwan
No abstract text is available yet for this article.
November 3, 2017: Ocular Immunology and Inflammation
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