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Limbal deficiency

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https://www.readbyqxmd.com/read/28816624/comparison-of-two-rabbit-models-with-deficiency-of-corneal-epithelium-and-limbal-stem-cells-established-by-different-methods
#1
Hening Zhang, Shaochun Lin, Min Zhang, Qijiong Li, Weihua Li, Wencong Wang, Minglei Zhao, Yaojue Xie, Zhiquan Li, Mian Huang, Zhichong Wang, Xiulan Zhang, Bing Huang
Limbal stem cell defect model is an important animal model that provides a basis for the study of ocular surface diseases. The rabbit cornea is of moderate size and is widely used in such studies as an experimental animal model. At present, the main modeling methods are alkali burns, and corneal limbus girdling and corneal epithelium doctoring. Each method has its own characteristics. In this study, we observed rabbit models with severe ocular surface defect established by the two methods and changes after amniotic membrane transplantation...
August 14, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28813511/differentiation-of-human-embryonic-stem-cells-into-corneal-epithelial-progenitor-cells-under-defined-conditions
#2
Canwei Zhang, Liqun Du, Kunpeng Pang, Xinyi Wu
The development of cell-based therapies using stem cells represents a significant breakthrough in the treatment of limbal stem cell deficiency (LSCD). The aim of this study was to develop a novel protocol to differentiate human embryonic stem cells (hESCs) into corneal epithelial progenitor cells (CEPCs), with similar features to primary cultured human limbal stem cells (LSCs), using a medium composed of DMEM/F12 and defined keratinocyte serum-free medium (KSFM) (1:1) under different carbon dioxide (CO2) levels in culture...
2017: PloS One
https://www.readbyqxmd.com/read/28758321/therapeutic-effect-of-human-adipose-tissue-derived-mesenchymal-stem-cells-in-experimental-corneal-failure-due-to-limbal-stem-cell-niche-damage
#3
Sara Galindo, José M Herreras, Marina López-Paniagua, Esther Rey, Ana de la Mata, María Plata-Cordero, Margarita Calonge, Teresa Nieto-Miguel
Limbal stem cells are responsible for the continuous renewal of the corneal epithelium. The destruction or dysfunction of these stem cells or their niche induces limbal stem cell deficiency (LSCD) leading to visual loss, chronic pain, and inflammation of the ocular surface. To restore the ocular surface in cases of bilateral LSCD, an extraocular source of stem cells is needed to avoid dependence on allogeneic limbal stem cells that are difficult to obtain, isolate, and culture. The aim of this work was to test the tolerance and the efficacy of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) to regenerate the ocular surface in two experimental models of LSCD that closely resemble the different severity grades of the human pathology...
July 31, 2017: Stem Cells
https://www.readbyqxmd.com/read/28715398/a-non-invasive-way-to-isolate-and-phenotype-cells-from-the-conjunctiva
#4
Tanima Bose, Aihua Hou, Ryan Lee, Louis Tong, K George Chandy
Traditionally, ocular surface cytology is studied with techniques such as spatula technology and brush technology. The problem with these techniques is that they may induce traumatic lesions on the surface of the eye, which can progress to scarring, eyelid deformity, limbal stem cell deficiency and in some cases, cause great discomfort to the subject. To avoid these clinical problems, impression cytology (IC) was developed to diagnose dry eye disease and later neoplasia, atopic disease, vernal keratoconjunctivitis and keratoconjunctivitis sicca...
July 5, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28700649/autophagy-mediates-cell-cycle-response-by-regulating-nucleocytoplasmic-transport-of-pax6-in-limbal-stem-cells-under-ultraviolet-a-stress
#5
Maria Laggner, Andreas Pollreisz, Gerald Schmidinger, Ursula Schmidt-Erfurth, Ying-Ting Chen
Limbal stem cells (LSC) account for homeostasis and regeneration of corneal epithelium. Solar ultraviolet A (UVA) is the major source causing oxidative damage in the ocular surface. Autophagy, a lysosomal degradation mechanism, is essential for physiologic function and stress defense of stem cells. PAX6, a master transcription factor governing corneal homeostasis by regulating cell cycle and cell fate of LSC, responds to oxidative stress by nucleocytoplasmic shuttling. Impaired autophagy and deregulated PAX6 have been reported in oxidative stress-related ocular surface disorders...
2017: PloS One
https://www.readbyqxmd.com/read/28700252/modified-allogenic-simple-limbal-epithelial-transplantation-followed-by-keratoplasty-as-treatment-for-total-limbal-stem-cell-deficiency
#6
Alfonso Vasquez-Perez, Mayank A Nanavaty
PURPOSE: To describe a modified allogenic simple limbal epithelial transplant (SLET) technique with large donor tissue explants followed by keratoplasty. METHODS: A 69-year-old with conjunctival melanoma on her left eye developed total limbal stem cell deficiency (LSCD) after multiple cycles of topical mitomycin. She also had herpes stromal keratitis while on treatment with mitomycin. She underwent modified allogenic SLET with large donor limbal tissue explants glued on the cornea directly and the amniotic membrane placed over the limbal explants...
July 12, 2017: Ocular Immunology and Inflammation
https://www.readbyqxmd.com/read/28665475/autologous-cultivated-limbal-stem-cell-transplantation-after-failed-previous-limbal-graft
#7
Karl A Knutsson, Stanislav Matuska, Paolo Rama
PURPOSE: To describe a case of unilateral limbal stem cell deficiency (LSCD) with previously failed autologous graft, resolved by ocular surface reconstruction using cultured autologous limbal stem cells from the contralateral eye. CASE REPORT: A 35-year-old patient presented to our clinic with LSCD due to a unilateral alkali burn. The patient had received a previous limbal graft from the contralateral eye that had failed to impede corneal conjunctivalization. We decided to repeat limbal stem cell transplantation using an ex vivo cultivation procedure to reduce the risk of tissue harvesting on the healthy fellow eye...
June 20, 2017: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/28644241/long-term-ocular-surface-stability-in-conjunctival-limbal-autograft-donor-eyes
#8
Albert Y Cheung, Enrica Sarnicola, Edward J Holland
PURPOSE: To investigate the incidence of limbal stem cell deficiency (LSCD) in donor eyes after conjunctival limbal autograft (CLAU). METHODS: An observational retrospective review was performed on all patients who underwent CLAU alone, combined keratolimbal allograft with CLAU ("Modified Cincinnati Procedure"), or combined living-related conjunctival limbal allograft (lr-CLAL) with CLAU having ≥6 months of follow-up after surgery. The outcome measures were best-corrected visual acuity (BCVA) and ocular surface status...
June 21, 2017: Cornea
https://www.readbyqxmd.com/read/28644237/outcomes-of-keratoplasty-after-ocular-surface-stem-cell-transplantation
#9
Lorena Sepsakos, Albert Y Cheung, Edward J Holland
PURPOSE: To investigate the long-term outcomes of penetrating keratoplasty (PK) and deep anterior lamellar keratoplasty (DALK) after ocular surface stem cell transplantation (OSST). METHODS: An observational retrospective review was performed on all patients who underwent OSST followed by either PK or DALK with a minimum of 6-month follow-up. RESULTS: One hundred two eyes fulfilled the inclusion criteria. The mean time to keratoplasty after OSST was 19 months, and the mean follow-up was 57 months after keratoplasty...
June 21, 2017: Cornea
https://www.readbyqxmd.com/read/28614157/probable-donor-derived-cytomegalovirus-disease-after-keratolimbal-allograft-transplantation
#10
Albert Y Cheung, Amit Govil, Scott R Friedstrom, Edward J Holland
PURPOSE: To report a case of probable donor-derived cytomegalovirus (CMV) infection after keratolimbal allograft (KLAL) transplantation. METHODS: Observational case report. RESULTS: A 41-year-old man with a history of aniridic keratopathy and limbal stem cell deficiency underwent KLAL in his right eye. Preoperatively, he was negative for CMV IgG and IgM. Postoperatively, he was maintained on tacrolimus and mycophenolate mofetil for systemic immunosuppression; he was also on prophylactic valganciclovir (for CMV) and trimethoprim/sulfamethoxazole (for pneumocystis pneumonia) for 1 month...
August 2017: Cornea
https://www.readbyqxmd.com/read/28586929/-keratoprosthesis-with-biological-haptic-the-d%C3%A3-sseldorf-offenburg-experience-with-osteo-odonto-and-tibia-keratoprosthesis
#11
Gerd Geerling, Konrad Hille
In severe ocular surface disease with limbal stem cell deficiency associated with severe dry eye, penetrating keratoplasty is likely to fail. In these cases, and provided the posterior segment is normal, keratoprosthesis surgery (KPro) can achieve visual rehabilitation. In KPros with an autologous biological haptic, a bone-tooth-complex (osteo-odonto-keratoprothesis, OOKPro) or a piece of tibial cortex (tibia-keratoprosthesis, TKPro) is used to carry a PMMA cylinder. This construct can successfully be implanted even in severely dry eyes and in those with autoimmune disease, in which other KPro designs frequently fail...
June 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/28582373/ocular-surface-changes-after-sulfur-mustard-exposure-in-rabbits-monitored-by-impression-cytology
#12
Shlomit Dachir, Hila Gutman, Ariel Gore, Liat Cohen, Maayan Cohen, Adina Amir, Vered Horwitz, Tamar Kadar
PURPOSE: Ocular injuries after exposure to sulfur mustard (SM) are characterized by acute corneal erosion and inflammation of the anterior segment that may be followed by delayed corneal neovascularization and epithelial defects, associated with limbal stem cell deficiency in part of the exposed eyes. This study aimed to further clarify the mechanism of the late injury by monitoring SM-induced cytological alterations in the ocular surface, in relation to the clinical symptoms, using impression cytology (IC)...
June 2, 2017: Cornea
https://www.readbyqxmd.com/read/28575918/-expansion-and-transplantation-of-limbal-stem-cells-for-corneal-surface-regeneration
#13
Adrian Gericke, Joanna Wasielica-Poslednik, Marion Zimmermann, Aytan Musayeva
In humans, regeneration of the corneal epithelium is regulated by the stem cell reservoir of the limbus. After extensive limbal damage, e.g., by inflammation, thermal burn or chemical injury, limbal stem cell deficiency leading to vascularization and opacification of the cornea and resulting in vision loss, may develop. Several techniques of autologous and allogenic stem cell transplantation have been established. The limbus can be restored by transplantation of whole limbal grafts, small limbal biopsies or by ex vivo-expanded limbal cells...
June 2, 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/28567305/characterization-of-ex-vivo-expanded-oral-mucosal-epithelium-cells-on-acellular-porcine-corneal-stroma-for-ocular-surface-reconstruction
#14
Jia-Song Wang, Hua-Tao Xie, Ming-Chang Zhang
PURPOSE: To ex vivo expand oral mucosal epithelium cells (OMECs) on acellular porcine corneal stroma (APCS) without using feeder cells and serum and to compare the morphologic and phenotypic characteristics of cultured oral cells on APCS to those of cells on deluded human amniotic membrane (HAM). METHODS: SD rat oral mucosal biopsies were cultured on APCS and HAM. Reverse-transcription polymerase chain reaction (RT-PCR) and immunohistochemistry were used to analyze the characterization of stem cells and epithelial differentiation of the outgrowth products...
2017: Journal of Ophthalmology
https://www.readbyqxmd.com/read/28538612/scleral-contact-lenses-in-an-academic-oculoplastics-clinic-epidemiology-and-emerging-considerations
#15
Harinder S Chahal, Marcela Estrada, Christine W Sindt, Jacob A Boehme, Mark A Greiner, Jeffrey A Nerad, Keith D Carter, Richard C Allen, Erin M Shriver
PURPOSE: To describe the role and efficacy of scleral contact lenses (SCLs) in the treatment of progressive keratopathy in patients who have undergone periocular surgical procedures, to investigate the financial impact of these surgical interventions, and to demonstrate the role of oculoplastic surgery in improving scleral contact lens fit. METHODS: A retrospective medical record review was performed to identify patients who both received SCLs and were examined by the oculoplastics service at the University of Iowa between January 1990 and December 2015...
May 22, 2017: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28528957/proteasomes-in-corneal-epithelial-cells-and-cultured-autologous-oral-mucosal-epithelial-cell-sheet-caomecs-graft-used-for-the-ocular-surface-regeneration
#16
Fawzia Bardag-Gorce, Richard Hoft, Imara Meepe, Julio Garcia, Kumar Tiger, Andrew Wood, Amanda Laporte, Derek Pan, Andrew Makalinao, Robert Niihara, Joan Oliva, Arjie Florentino, Amber M Gorce, Jeremy Stark, Daileen Cortez, Samuel W French, Yutaka Niihara
PURPOSE: This study focuses on characterizing proteasomes in corneal epithelial cells (CEC) and in cultured autologous oral mucosal epithelial cell sheets (CAOMECS) used to regenerate the ocular surface. METHODS: Limbal stem cell deficiency (LSCD) was surgically induced in rabbit corneas. CAOMECS was engineered and grafted onto corneas with LSCD to regenerate the ocular surface. RESULTS: LSCD caused an increase in inflammatory cells in the ocular surface, an increase in the formation of immunoproteasomes (IPR), and a decrease in the formation of constitutive proteasome (CPR)...
May 18, 2017: Ocular Surface
https://www.readbyqxmd.com/read/28510772/prevalence-incidence-and-risk-factors-for-the-development-of-glaucoma-in-patients-with-aniridia
#17
Shantha Balekudaru, Nandhini Sankaranarayanan, Sumita Agarkar
PURPOSE: To assess the prevalence, incidence, and risk factors for the development of glaucoma in patients with aniridia. METHODS: Retrospective analysis of case records of patients diagnosed as having congenital aniridia between January 1986 and December 2011 was performed. Patients with a follow-up of more than 12 months were included. RESULTS: Ninety-one patients (180 eyes) with the diagnosis of aniridia were identified from the case records...
May 15, 2017: Journal of Pediatric Ophthalmology and Strabismus
https://www.readbyqxmd.com/read/28484851/-long-term-outcome-of-keratoprosthesis-with-biological-support
#18
K Hille
BACKGROUND: In severe Sicca syndrome and limbal stem cell deficiency, vision can be restored by an artificial optical implant into the cornea (keratoprosthesis, KPro). After multiple efforts to achieve long-lasting corneal fixation of such an optical implant, two methods have become established: KPro with biological support, such as osteo-odonto-keratoprosthesis (OOKP) made from the root of patient's own tooth or osteo-keratoprosthesis (OKP), made from cortical bone of the patient's tibia; and the Boston KPro...
May 8, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/28465692/safety-of-cultivated-limbal-epithelial-stem-cell-transplantation-for-human-corneal-regeneration
#19
REVIEW
J Behaegel, S Ní Dhubhghaill, C Koppen, N Zakaria
Ex vivo cultivated limbal stem cell transplantation is a promising technique for the treatment of limbal stem cell deficiency. While the results of the clinical trials have been extensively reported since the introduction of the technique in 1997, little has been reported regarding the potential health risks associated with production processes and transplantation techniques. Culture procedures require the use of animal and/or human-derived products, which carry the potential of introducing toxic or infectious agents through contamination with known or unknown additives...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28440250/outcomes-of-deep-anterior-lamellar-keratoplasty-following-autologous-simple-limbal-epithelial-transplant-in-pediatric-unilateral-severe-chemical-injury
#20
Divya Singh, M Vanathi, Chanchal Gupta, Noopur Gupta, Radhika Tandon
AIMS: To evaluate outcomes of deep anterior lamellar keratoplasty (DALK) in pediatric eyes with unilateral severe chemical injury which have undergone autologous simple limbal epithelial transplant (SLET). SETTINGS AND DESIGN: The study design was a retrospective case series. MATERIALS AND METHODS: This retrospective, case series of all children <16 years of age that have undergone DALK following autologous SLET procedure in unilateral severe chemical injury evaluates the outcomes and complications in the setting of a tertiary care center in North India...
March 2017: Indian Journal of Ophthalmology
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