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Limbal deficiency

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https://www.readbyqxmd.com/read/28715398/a-non-invasive-way-to-isolate-and-phenotype-cells-from-the-conjunctiva
#1
Tanima Bose, Aihua Hou, Ryan Lee, Louis Tong, K George Chandy
Traditionally, ocular surface cytology is studied with techniques such as spatula technology and brush technology. The problem with these techniques is that they may induce traumatic lesions on the surface of the eye, which can progress to scarring, eyelid deformity, limbal stem cell deficiency and in some cases, cause great discomfort to the subject. To avoid these clinical problems, impression cytology (IC) was developed to diagnose dry eye disease and later neoplasia, atopic disease, vernal keratoconjunctivitis and keratoconjunctivitis sicca...
July 5, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28700649/autophagy-mediates-cell-cycle-response-by-regulating-nucleocytoplasmic-transport-of-pax6-in-limbal-stem-cells-under-ultraviolet-a-stress
#2
Maria Laggner, Andreas Pollreisz, Gerald Schmidinger, Ursula Schmidt-Erfurth, Ying-Ting Chen
Limbal stem cells (LSC) account for homeostasis and regeneration of corneal epithelium. Solar ultraviolet A (UVA) is the major source causing oxidative damage in the ocular surface. Autophagy, a lysosomal degradation mechanism, is essential for physiologic function and stress defense of stem cells. PAX6, a master transcription factor governing corneal homeostasis by regulating cell cycle and cell fate of LSC, responds to oxidative stress by nucleocytoplasmic shuttling. Impaired autophagy and deregulated PAX6 have been reported in oxidative stress-related ocular surface disorders...
2017: PloS One
https://www.readbyqxmd.com/read/28700252/modified-allogenic-simple-limbal-epithelial-transplantation-followed-by-keratoplasty-as-treatment-for-total-limbal-stem-cell-deficiency
#3
Alfonso Vasquez-Perez, Mayank A Nanavaty
PURPOSE: To describe a modified allogenic simple limbal epithelial transplant (SLET) technique with large donor tissue explants followed by keratoplasty. METHODS: A 69-year-old with conjunctival melanoma on her left eye developed total limbal stem cell deficiency (LSCD) after multiple cycles of topical mitomycin. She also had herpes stromal keratitis while on treatment with mitomycin. She underwent modified allogenic SLET with large donor limbal tissue explants glued on the cornea directly and the amniotic membrane placed over the limbal explants...
July 12, 2017: Ocular Immunology and Inflammation
https://www.readbyqxmd.com/read/28665475/autologous-cultivated-limbal-stem-cell-transplantation-after-failed-previous-limbal-graft
#4
Karl A Knutsson, Stanislav Matuska, Paolo Rama
PURPOSE: To describe a case of unilateral limbal stem cell deficiency (LSCD) with previously failed autologous graft, resolved by ocular surface reconstruction using cultured autologous limbal stem cells from the contralateral eye. CASE REPORT: A 35-year-old patient presented to our clinic with LSCD due to a unilateral alkali burn. The patient had received a previous limbal graft from the contralateral eye that had failed to impede corneal conjunctivalization. We decided to repeat limbal stem cell transplantation using an ex vivo cultivation procedure to reduce the risk of tissue harvesting on the healthy fellow eye...
June 20, 2017: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/28644241/long-term-ocular-surface-stability-in-conjunctival-limbal-autograft-donor-eyes
#5
Albert Y Cheung, Enrica Sarnicola, Edward J Holland
PURPOSE: To investigate the incidence of limbal stem cell deficiency (LSCD) in donor eyes after conjunctival limbal autograft (CLAU). METHODS: An observational retrospective review was performed on all patients who underwent CLAU alone, combined keratolimbal allograft with CLAU ("Modified Cincinnati Procedure"), or combined living-related conjunctival limbal allograft (lr-CLAL) with CLAU having ≥6 months of follow-up after surgery. The outcome measures were best-corrected visual acuity (BCVA) and ocular surface status...
June 21, 2017: Cornea
https://www.readbyqxmd.com/read/28644237/outcomes-of-keratoplasty-after-ocular-surface-stem-cell-transplantation
#6
Lorena Sepsakos, Albert Y Cheung, Edward J Holland
PURPOSE: To investigate the long-term outcomes of penetrating keratoplasty (PK) and deep anterior lamellar keratoplasty (DALK) after ocular surface stem cell transplantation (OSST). METHODS: An observational retrospective review was performed on all patients who underwent OSST followed by either PK or DALK with a minimum of 6-month follow-up. RESULTS: One hundred two eyes fulfilled the inclusion criteria. The mean time to keratoplasty after OSST was 19 months, and the mean follow-up was 57 months after keratoplasty...
June 21, 2017: Cornea
https://www.readbyqxmd.com/read/28614157/probable-donor-derived-cytomegalovirus-disease-after-keratolimbal-allograft-transplantation
#7
Albert Y Cheung, Amit Govil, Scott R Friedstrom, Edward J Holland
PURPOSE: To report a case of probable donor-derived cytomegalovirus (CMV) infection after keratolimbal allograft (KLAL) transplantation. METHODS: Observational case report. RESULTS: A 41-year-old man with a history of aniridic keratopathy and limbal stem cell deficiency underwent KLAL in his right eye. Preoperatively, he was negative for CMV IgG and IgM. Postoperatively, he was maintained on tacrolimus and mycophenolate mofetil for systemic immunosuppression; he was also on prophylactic valganciclovir (for CMV) and trimethoprim/sulfamethoxazole (for pneumocystis pneumonia) for 1 month...
August 2017: Cornea
https://www.readbyqxmd.com/read/28586929/-keratoprosthesis-with-biological-haptic-the-d%C3%A3-sseldorf-offenburg-experience-with-osteo-odonto-and-tibia-keratoprosthesis
#8
Gerd Geerling, Konrad Hille
In severe ocular surface disease with limbal stem cell deficiency associated with severe dry eye, penetrating keratoplasty is likely to fail. In these cases, and provided the posterior segment is normal, keratoprosthesis surgery (KPro) can achieve visual rehabilitation. In KPros with an autologous biological haptic, a bone-tooth-complex (osteo-odonto-keratoprothesis, OOKPro) or a piece of tibial cortex (tibia-keratoprosthesis, TKPro) is used to carry a PMMA cylinder. This construct can successfully be implanted even in severely dry eyes and in those with autoimmune disease, in which other KPro designs frequently fail...
June 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/28582373/ocular-surface-changes-after-sulfur-mustard-exposure-in-rabbits-monitored-by-impression-cytology
#9
Shlomit Dachir, Hila Gutman, Ariel Gore, Liat Cohen, Maayan Cohen, Adina Amir, Vered Horwitz, Tamar Kadar
PURPOSE: Ocular injuries after exposure to sulfur mustard (SM) are characterized by acute corneal erosion and inflammation of the anterior segment that may be followed by delayed corneal neovascularization and epithelial defects, associated with limbal stem cell deficiency in part of the exposed eyes. This study aimed to further clarify the mechanism of the late injury by monitoring SM-induced cytological alterations in the ocular surface, in relation to the clinical symptoms, using impression cytology (IC)...
June 2, 2017: Cornea
https://www.readbyqxmd.com/read/28575918/-expansion-and-transplantation-of-limbal-stem-cells-for-corneal-surface-regeneration
#10
Adrian Gericke, Joanna Wasielica-Poslednik, Marion Zimmermann, Aytan Musayeva
In humans, regeneration of the corneal epithelium is regulated by the stem cell reservoir of the limbus. After extensive limbal damage, e.g., by inflammation, thermal burn or chemical injury, limbal stem cell deficiency leading to vascularization and opacification of the cornea and resulting in vision loss, may develop. Several techniques of autologous and allogenic stem cell transplantation have been established. The limbus can be restored by transplantation of whole limbal grafts, small limbal biopsies or by ex vivo-expanded limbal cells...
June 2, 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/28567305/characterization-of-ex-vivo-expanded-oral-mucosal-epithelium-cells-on-acellular-porcine-corneal-stroma-for-ocular-surface-reconstruction
#11
Jia-Song Wang, Hua-Tao Xie, Ming-Chang Zhang
PURPOSE: To ex vivo expand oral mucosal epithelium cells (OMECs) on acellular porcine corneal stroma (APCS) without using feeder cells and serum and to compare the morphologic and phenotypic characteristics of cultured oral cells on APCS to those of cells on deluded human amniotic membrane (HAM). METHODS: SD rat oral mucosal biopsies were cultured on APCS and HAM. Reverse-transcription polymerase chain reaction (RT-PCR) and immunohistochemistry were used to analyze the characterization of stem cells and epithelial differentiation of the outgrowth products...
2017: Journal of Ophthalmology
https://www.readbyqxmd.com/read/28538612/scleral-contact-lenses-in-an-academic-oculoplastics-clinic-epidemiology-and-emerging-considerations
#12
Harinder S Chahal, Marcela Estrada, Christine W Sindt, Jacob A Boehme, Mark A Greiner, Jeffrey A Nerad, Keith D Carter, Richard C Allen, Erin M Shriver
PURPOSE: To describe the role and efficacy of scleral contact lenses (SCLs) in the treatment of progressive keratopathy in patients who have undergone periocular surgical procedures, to investigate the financial impact of these surgical interventions, and to demonstrate the role of oculoplastic surgery in improving scleral contact lens fit. METHODS: A retrospective medical record review was performed to identify patients who both received SCLs and were examined by the oculoplastics service at the University of Iowa between January 1990 and December 2015...
May 22, 2017: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28528957/proteasomes-in-corneal-epithelial-cells-and-cultured-autologous-oral-mucosal-epithelial-cell-sheet-caomecs-graft-used-for-the-ocular-surface-regeneration
#13
Fawzia Bardag-Gorce, Richard Hoft, Imara Meepe, Julio Garcia, Kumar Tiger, Andrew Wood, Amanda Laporte, Derek Pan, Andrew Makalinao, Robert Niihara, Joan Oliva, Arjie Florentino, Amber M Gorce, Jeremy Stark, Daileen Cortez, Samuel W French, Yutaka Niihara
PURPOSE: This study focuses on characterizing proteasomes in corneal epithelial cells (CEC) and in cultured autologous oral mucosal epithelial cell sheets (CAOMECS) used to regenerate the ocular surface. METHODS: Limbal stem cell deficiency (LSCD) was surgically induced in rabbit corneas. CAOMECS was engineered and grafted onto corneas with LSCD to regenerate the ocular surface. RESULTS: LSCD caused an increase in inflammatory cells in the ocular surface, an increase in the formation of immunoproteasomes (IPR), and a decrease in the formation of constitutive proteasome (CPR)...
May 18, 2017: Ocular Surface
https://www.readbyqxmd.com/read/28510772/prevalence-incidence-and-risk-factors-for-the-development-of-glaucoma-in-patients-with-aniridia
#14
Shantha Balekudaru, Nandhini Sankaranarayanan, Sumita Agarkar
PURPOSE: To assess the prevalence, incidence, and risk factors for the development of glaucoma in patients with aniridia. METHODS: Retrospective analysis of case records of patients diagnosed as having congenital aniridia between January 1986 and December 2011 was performed. Patients with a follow-up of more than 12 months were included. RESULTS: Ninety-one patients (180 eyes) with the diagnosis of aniridia were identified from the case records...
May 15, 2017: Journal of Pediatric Ophthalmology and Strabismus
https://www.readbyqxmd.com/read/28484851/-long-term-outcome-of-keratoprosthesis-with-biological-support
#15
K Hille
BACKGROUND: In severe Sicca syndrome and limbal stem cell deficiency, vision can be restored by an artificial optical implant into the cornea (keratoprosthesis, KPro). After multiple efforts to achieve long-lasting corneal fixation of such an optical implant, two methods have become established: KPro with biological support, such as osteo-odonto-keratoprosthesis (OOKP) made from the root of patient's own tooth or osteo-keratoprosthesis (OKP), made from cortical bone of the patient's tibia; and the Boston KPro...
May 8, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/28465692/safety-of-cultivated-limbal-epithelial-stem-cell-transplantation-for-human-corneal-regeneration
#16
REVIEW
J Behaegel, S Ní Dhubhghaill, C Koppen, N Zakaria
Ex vivo cultivated limbal stem cell transplantation is a promising technique for the treatment of limbal stem cell deficiency. While the results of the clinical trials have been extensively reported since the introduction of the technique in 1997, little has been reported regarding the potential health risks associated with production processes and transplantation techniques. Culture procedures require the use of animal and/or human-derived products, which carry the potential of introducing toxic or infectious agents through contamination with known or unknown additives...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28440250/outcomes-of-deep-anterior-lamellar-keratoplasty-following-autologous-simple-limbal-epithelial-transplant-in-pediatric-unilateral-severe-chemical-injury
#17
Divya Singh, M Vanathi, Chanchal Gupta, Noopur Gupta, Radhika Tandon
AIMS: To evaluate outcomes of deep anterior lamellar keratoplasty (DALK) in pediatric eyes with unilateral severe chemical injury which have undergone autologous simple limbal epithelial transplant (SLET). SETTINGS AND DESIGN: The study design was a retrospective case series. MATERIALS AND METHODS: This retrospective, case series of all children <16 years of age that have undergone DALK following autologous SLET procedure in unilateral severe chemical injury evaluates the outcomes and complications in the setting of a tertiary care center in North India...
March 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28426442/amniotic-membrane-use-for-management-of-corneal-limbal-stem-cell-deficiency
#18
REVIEW
Alfonso L Sabater, Victor L Perez
PURPOSE OF REVIEW: The current article reviews the most recent surgical techniques for management of corneal limbal stem cell deficiency (LSCD) using amniotic membrane tissue. RECENT FINDINGS: Early successes with amniotic membrane transplantation (AMT) for the treatment of ocular surface disorders have encouraged clinicians to investigate new applications. The use of AMT as a temporary patch in emergency cases in which LSCD may develop has considerably improved the prognosis of these patients...
July 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28426441/diagnosis-of-corneal-limbal-stem-cell-deficiency
#19
REVIEW
Kyeong Hwan Kim, Shahzad I Mian
PURPOSE OF REVIEW: A state of limbal stem cell deficiency (LSCD) can be secondary to a number of etiologies, resulting in either a reduction in the total number of limbal stem cells or an abnormality in stem cell function. Initially, the epithelium becomes irregular and hazy; however, this condition may progress to persistent corneal epithelial defects, stromal scarring, ulceration, and even perforation. Since LSCD secondary to a variety of etiologies may be reversible, and various factors are prognostic of disease progression, timely diagnosis is important...
July 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28406800/simple-limbal-epithelial-transplantation
#20
REVIEW
Virender S Sangwan, John A H Sharp
PURPOSE OF REVIEW: Simple limbal epithelial transplant (SLET) is a technique for addressing limbal stem cell deficiency. Limbal tissue from a donor eye, typically the patient's fellow healthy eye, is transplanted onto an amniotic membrane attached to the surface of the diseased eye. SLET was developed to address limitations of other techniques, specifically the technical difficulty of ex-vivo expansion of cells required in some techniques and the larger amount of valuable limbal tissue harvested in techniques not relying on ex-vivo expansion...
July 2017: Current Opinion in Ophthalmology
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