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Amniotic membran

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https://www.readbyqxmd.com/read/28637100/women-s-expectations-and-experiences-of-rupture-of-membranes-and-views-of-the-potential-use-of-reagent-pads-for-detecting-amniotic-fluid
#1
Helen Spiby, Sara Borrelli, Anita J Hughes
AIMS: To explore first time mothers' expectations and experiences regarding rupture of membranes at term and their views on the potential use of reagent pads that detect amniotic fluid. BACKGROUND: There is little information available on women's experiences of spontaneous rupture of membranes, or interest in using methods to confirm rupture of membranes (e.g. reagent pads). DESIGN: Descriptive qualitative study, using focus groups and telephone interviews with women during pregnancy and after the birth of their first baby...
June 21, 2017: Journal of Advanced Nursing
https://www.readbyqxmd.com/read/28622703/qualitative-determination-of-human-chorionic-gonadotropin-in-vaginal-washings-for-the-early-diagnosis-of-premature-rupture-of-fetal-membranes
#2
Camila Buziquia Dartibale, Nelson Shozo Uchimura, Luiz Nery, Angelita Polato Schumeish, Liza Yurie Teruya Uchimura, Rosangela Getirana Santana, Taqueco Teruya Uchimura
Purpose This study aimed to evaluate and validate the qualitative human chorionic gonadotropin β subunit (β-hCG) test of the vaginal fluid washings of pregnant women with premature rupture of fetal membranes (PROM). Methods Cross-sectional study of pregnant women between gestational weeks 24 and 39 who underwent consultations in one of our institutions. They were divided into two groups: group A (pregnant women clinically diagnosed with PROM) and group B (pregnant women without loss of amniotic liquid)...
June 16, 2017: Revista Brasileira de Ginecologia e Obstetrícia
https://www.readbyqxmd.com/read/28621502/amniotic-membrane-stimulates-cell-migration-by-modulating-transforming-growth-factor-%C3%AE-signaling
#3
Catalina Ruiz-Cañada, Ángel Bernabé-García, Sergio Liarte, Carmen Luisa Insausti, Diego Angosto, José María Moraleda, Gregorio Castellanos, Francisco José Nicolás
Keratinocyte migration is a mandatory aspect of wound-healing. We have previously shown that Amniotic Membrane (AM) applied to chronic wounds assists healing through a process resulting in overexpression of c-Jun at the wound's leading edge. Also, we have demonstrated that amniotic membrane modifies the genetic program induced by TGF-ß in chronic wounds. In this paper, we used a scratch assay of Mv1Lu and HaCaT cells to examine the influence of AM application on the underlying signaling during scratch closure...
June 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28618920/castor-oil-for-induction-of-labour-a-retrospective-study
#4
Isabella Neri, Giulia Dante, Lucrezia Pignatti, Chiara Salvioli, Fabio Facchinetti
PURPOSE: The aim of this study is to investigate the safety and efficacy of castor oil to induce labour. MATERIALS AND METHODS: A retrospective observational case control study was conducted over five years. Castor oil was proposed to women referred to the Birth Centre (Castor Oil group (COG)). They were compared to women who chose to be followed by the traditional doctor-led unit (control group (CG)). Castor oil was administered in a 60 ml single dose in 200 ml of warm water...
June 15, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28616359/bioactive-antimicrobial-peptides-as-therapeutics-for-corneal-wounds-and-infections
#5
REVIEW
Gina L Griffith, Anne Kasus-Jacobi, H Anne Pereira
Significance: More than 2 million eye injuries and infections occur each year in the United States that leave civilians and military members with reduced or complete vision loss due to the lack of effective therapeutics. Severe ocular injuries and infections occur in varied settings including the home, workplace, and battlefields. In this review, we discuss the potential of developing antimicrobial peptides (AMPs) as therapeutics for the treatment of corneal wounds and infections for which the current treatment options are inadequate...
June 1, 2017: Advances in Wound Care
https://www.readbyqxmd.com/read/28611933/monochorionic-triamniotic-triplet-pregnancy-complicated-by-twin-reversed-arterial-perfusion-sequence-case-report-and-literature-review
#6
Pingshan Pan, Guoyang Luo, Lu Tang, Jeanne D Rolle, Yuqin Qin, Quan Zeng, Jiangting Wei, Yuanfang Chen, Hongwei Wei
Background  Monochorionic-triamniotic pregnancies are rare and fraught with complications. Case  A case of monochorionic-triamniotic triplet pregnancy complicated by twin reversed arterial perfusion (TRAP) sequence is presented. The patient declined termination or selective fetal reduction. Triplet C was acardiac. At 24 weeks, triplet B developed polyhydramnios. At 30 weeks, polyhydramnios was seen in all three amniotic sacs, but without signs of fetal hydrops and with normal Doppler velocimetry measurements in the umbilical artery, middle cerebral artery, and ductus arteriosus of triplets A and B...
April 2017: American Journal of Perinatology Reports
https://www.readbyqxmd.com/read/28605672/intercellular-transfer-of-organelles-during-body-pigmentation
#7
REVIEW
Ryosuke Tadokoro, Yoshiko Takahashi
The intercellular transfer of the melanin-producing organelle, called melanosome, from melanocytes to adjacent keratinocytes, is largely responsible for the coat colors and skin pigmentation of amniotes (birds, reptiles, and mammals). Although several hypotheses of melanin-transfer were proposed mainly by in vitro studies and electron microscopies, how the melanosome transfer takes place in the actual skin remained unclear. With advances in technologies of gene manipulations and high-resolution microscopy that allow direct visualization of plasma membrane, we are beginning to understand the amazing behaviors and dynamics of melanocytes...
June 9, 2017: Current Opinion in Genetics & Development
https://www.readbyqxmd.com/read/28602048/injected-corticosteroids-for-treating-plantar-heel-pain-in-adults
#8
REVIEW
Judy A David, Venkatesan Sankarapandian, Prince Rh Christopher, Ahana Chatterjee, Ashish S Macaden
BACKGROUND: Plantar heel pain, commonly resulting from plantar fasciitis, often results in significant morbidity. Treatment options include nonsteroidal anti-inflammatory drugs (NSAIDs), orthoses, physical therapy, physical agents (e.g. extracorporeal shock wave therapy (ESWT), laser) and invasive procedures including steroid injections. OBJECTIVES: To assess the effects (benefits and harms) of injected corticosteroids for treating plantar heel pain in adults. SEARCH METHODS: We searched the Cochrane Bone, Joint and Muscle Trauma Group Specialised Register, the Cochrane Central Register of Controlled Trials (the Cochrane Library), MEDLINE, Embase, CINAHL, clinical trials registries and conference proceedings...
June 11, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28599393/hepar-uterinum-a-history-of-ideas-on-fetal-nutrition
#9
Michael Obladen
The means of fetal nutrition has been debated for over two millennia, with the controversy of oral versus parenteral nutrition already in the Corpus Hippocraticum. In 1587 Aranzio rejected connections between maternal and fetal blood vessels, and coined the term "hepar uterinum" for the placenta. From the 16th to the 18th century, a fervent debate focused on the type and extent of connection between maternal and fetal vessels, which was finally settled by Hunter's injection experiment in 1774. But up to the middle of the 19th century, an important nutritive function was attributed to amniotic fluid...
May 24, 2017: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/28591956/-effects-of-human-acellular-amniotic-membrane-on-postsurgical-recovery-of-nail-beds
#10
Si-Liang Xue, Gui-Gui Chen, Xi Lü, Rong Mei
OBJECTIVES: To determine the effects of human acellular amniotic membrane(HAAM) on postsurgical recovery of nail beds. METHODS: Patients undergoing nail surgery were randomly divided into two groups: 19 in the experimental and 19 in the control groups. Patients in the experimental group were given HAAM before the wounds were covered with vaseline gauze the next day after surgery; whereas those in the control group received vaseline gauze dressing only. Ratings of pain were recorded when 1, 4, 8, 12, 24, 36, 48 and 72 h after the dressing was changed...
July 2016: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28588850/case-report-of-myometrial-window-following-fetoscopic-treatment-of-twin-twin-transfusion-syndrome-indications-of-underlying-collagen-vascular-disease
#11
Michael V Zaretsky, David K Manchester, Henry L Galan, Nicholas J Behrendt, Ahmed I Marwan, Kenneth W Liechty, Timothy M Crombleholme
Intraperitoneal amniotic fluid leak is a known complication of fetoscopic procedures that usually resolves spontaneously with expectant management. Intraperitoneal amniotic fluid leak may persist after fetoscopic procedures due to a myometrial window as well as to persistent chorioamniotic membrane disruption, which may be amenable to surgical repair.
June 2017: Clinical Case Reports
https://www.readbyqxmd.com/read/28586928/-biomaterials-or-donor-tissue-what-is-the-future-of-tissue-engenieering-for-cornea-reconstruction
#12
Björn O Bachmann, Stefan Schrader
For the replacement of corneal tissue, corneal grafts or amniotic membrane are still used as a standard material. Since this is biological tissue, there is only a limited standardization regarding preparation, tissue properties and behaviour after transplantation. In addition, there is a risk of disease transmission, and the availability of both human corneas and amniotic membrane is insufficient in many regions of the world, which is why alternative biomaterials have been explored for many years now. Among the natural biomaterials, materials based on collagen or keratin provide characteristics that make them good candidates for corneal tissue replacement...
June 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/28570820/human-milk-oligosaccharides-exhibit-antimicrobial-and-anti-biofilm-properties-against-group-b-streptococcus
#13
Dorothy L Ackerman, Ryan S Doster, Jörn-Hendrik Weitkamp, David Aronoff, Jennifer A Gaddy, Steven D Townsend
Streptococcus agalactiae (Group B Streptococcus, GBS) is a Gram-positive bacterial pathogen that causes invasive infections of both children and adults. During pregnancy, GBS is a significant cause of infection of the fetal membranes (chorioamnionitis), which can lead to intra-amniotic infection, preterm birth, stillbirth, and neonatal sepsis. Recently, breastfeeding has been thought to represent a potential mode of GBS transmission from mother to newborn, which might increase the risk for late-onset sepsis...
June 1, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28567305/characterization-of-ex-vivo-expanded-oral-mucosal-epithelium-cells-on-acellular-porcine-corneal-stroma-for-ocular-surface-reconstruction
#14
Jia-Song Wang, Hua-Tao Xie, Ming-Chang Zhang
PURPOSE: To ex vivo expand oral mucosal epithelium cells (OMECs) on acellular porcine corneal stroma (APCS) without using feeder cells and serum and to compare the morphologic and phenotypic characteristics of cultured oral cells on APCS to those of cells on deluded human amniotic membrane (HAM). METHODS: SD rat oral mucosal biopsies were cultured on APCS and HAM. Reverse-transcription polymerase chain reaction (RT-PCR) and immunohistochemistry were used to analyze the characterization of stem cells and epithelial differentiation of the outgrowth products...
2017: Journal of Ophthalmology
https://www.readbyqxmd.com/read/28559354/rna-seq-reveals-conservation-of-function-among-the-yolk-sacs-of-human-mouse-and-chicken
#15
Tereza Cindrova-Davies, Eric Jauniaux, Michael G Elliot, Sungsam Gong, Graham J Burton, D Stephen Charnock-Jones
The yolk sac is phylogenetically the oldest of the extraembryonic membranes. The human embryo retains a yolk sac, which goes through primary and secondary phases of development, but its importance is controversial. Although it is known to synthesize proteins, its transport functions are widely considered vestigial. Here, we report RNA-sequencing (RNA-seq) data for the human and murine yolk sacs and compare those data with data for the chicken. We also relate the human RNA-seq data to proteomic data for the coelomic fluid bathing the yolk sac...
May 30, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28557902/cryopreserved-amniotic-membrane-improves-clinical-outcomes-following-microdiscectomy
#16
D Greg Anderson, Victor Popov, Andrew L Raines, Julie O'Connell
STUDY DESIGN: Prospective, randomized controlled trial. OBJECTIVE: To compare pain, physical/mental functional recovery and recurrent herniation for patients following lumbar microdiscectomy with and without the use of a cryopreserved amniotic tissue graft. SUMMARY OF BACKGROUND DATA: Although microdiscectomy procedures are routinely successful for patients with lumbar radiculopathy due to herniated disc disease, residual low back pain, and recurrent herniation remain unsolved clinical problems...
May 26, 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/28553162/bladder-reconstruction-with-human-amniotic-membrane-in-a-xenograft-rat-model-a-preclinical-study
#17
Dimitri Barski, Holger Gerullis, Thorsten Ecke, Jin Yang, Gabriella Varga, Mihaly Boros, Isabel Pintelon, Jean-Pierre Timmermans, Thomas Otto
Background: Human amniotic membranes (HAMs) are assumed to have a number of unique characteristics including durability, hypoallergenic and anti-inflammatory properties. Materials and Methods: Multilayer HAMs from caesarian sections were applied to repair defined bladder defects in male Sprague-Dawley rats. The animals were sacrificed at 7, 21 and 42 days after implantation. Bladder volume capacity after grafting was measured. Histological analyses were performed to asses a number of parameters including HAM degradation, inflammatory reaction, graft rejection and smooth muscle ingrowth...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28550448/towards-xeno-free-cultures-of-human-limbal-stem-cells-for-ocular-surface-reconstruction
#18
Zala Lužnik, Claudia Breda, Vanessa Barbaro, Stefano Ferrari, Angelo Migliorati, Enzo Di Iorio, Barbara Ferrari, Carlo Griffoni, Andrea Grassetto, Hossein Mostafa Elbadawy, Marina Bertolin
Isolated limbal epithelial stem cells (LESCs) were cultured with or without a 3T3 murine fibroblast feeder-layer (FL) in 4 different culture media on culture plates or on denuded human amniotic membrane (AM) support and fibrin gel support: (1) control medium supplemented with fetal bovine serum; (2) control medium supplemented with the synthetic serum "XerumFree™ XF205" (XF); (3) CnT-20 medium supplemented with "XerumFree™ XF205" (CnT-XF) and (4) CnT-20 medium supplemented with human AB serum (CnT-AB). The three xenogeneic media were compared to standard condition (control + FL) and parameters assessed included cell morphology, proliferative potential, number of passages, assessment of clonogenic and abortive colonies, life span, ∆Np63α expression and epithelial morphology on AM...
May 26, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28548553/human-amniotic-membrane-is-not-suitable-for-the-grafting-of-colon-lesions-and-prevention-of-adhesions-in-a-xenograft-rat-model
#19
Dimitri Barski, Holger Gerullis, Thorsten Ecke, Gabriella Varga, Mihaly Boros, Isabel Pintelon, Jean-Pierre Timmermans, Thomas Otto
INTRODUCTION: New biological materials are needed for specific applications in reconstructive bowel surgery and for the prevention of adhesion formation. Amniotic membranes (AMs) are assumed to have a number of unique characteristics that enhance the ingrowth of the surrounding tissue. The aim of the present study was to provide proof of these qualities in a xenograft model. MATERIALS AND METHODS: A multilayer human AM (HAM) was applied to repair defined colon wall defects in Sprague-Dawley rats (n = 18)...
May 1, 2017: Surgical Innovation
https://www.readbyqxmd.com/read/28544462/biomimetic-macroporous-pcl-scaffolds-for-ex-vivo-expansion-of-cord-blood-derived-cd34-cells-with-feeder-cells-support
#20
Xiuwei Pan, Qiong Sun, Yuanhao Zhang, Haibo Cai, Yun Gao, Yongjia Shen, Weian Zhang
Ex vivo expansion of hematopoietic stem cells (HSCs) with most current methods can hardly satisfy clinical application requirement. While in vivo, HSCs efficiently self-renew in niche where they interact with 3D extracellular matrix and stromal cells. Therefore, co-cultures of CD34(+) cells and mesenchyme stem cells derived from human amniotic membrane (hAMSCs) on the basis of biomimetic macroporous three-dimensional (3D) poly(ε-caprolactone) (PCL) scaffolds are developed, where scaffolds and hAMSCs are applied to mimic structural and cellular microenvironment of HSCs...
May 24, 2017: Macromolecular Bioscience
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