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Amniotic membran

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https://www.readbyqxmd.com/read/29208979/induction-of-antimicrobial-peptides-secretion-by-il-1%C3%AE-enhances-human%C3%A2-amniotic-membrane-for-regenerative-medicine
#1
Fatemeh A Tehrani, Khashayar Modaresifar, Sara Azizian, Hassan Niknejad
Due to antibacterial characteristic, amnion has been frequently used in different clinical situations. Developing an in vitro method to augment endogenous antibacterial ingredient of amniotic epithelial and mesenchymal stem cells is desirable for a higher efficacy of this promising biomaterial. In this study, epithelial or mesenchymal side dependent effect of amniotic membrane (AM) on antibacterial activity against some laboratory and clinical isolated strains was investigated by modified disk diffusion method and colony count assay...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29204944/comparison-of-culture-media-indicates-a-role-for-autologous-serum-in-enhancing-phenotypic-preservation-of-rabbit-limbal-stem-cells-in-explant-culture
#2
Mehmet Gürdal, Özlem Barut Selver, Kemal Baysal, İsmet Durak
In this study, we aimed to compare the effects of six different cell culture media and autologous serum (AS) on the phenotypic characteristics of rabbit limbal epithelial stem cells (LESC) cultivated on porous polyethylene terephthalate (PET) membranes. Limbal explants from rabbit corneas were grown on PET membrane inserts in five different media: DMEM-F12 with fetal bovine serum (FBS) (DMEM-F12-FBS), with pluripotin (DMEM-F12-pluripotin) and with autologous serum (DMEM-F12-AS), Epilife, Keratinocyte Serum Free Medium (KSFM) and Defined-Keratinocyte Serum Free Medium...
December 4, 2017: Cytotechnology
https://www.readbyqxmd.com/read/29204836/fetal-membranes-derived-stem-cells-microenvironment
#3
Phelipe Oliveira Favaron, Maria Angelica Miglino
Recently, the regenerative medicine has been trying to congregate different areas such as tissue engineering and cellular therapy, in order to offer effective treatments to overcome several human and veterinary medical problems. In this regard, fetal membranes have been proposed as a powerful source for obtainment of multipotent stem cells with low immunogenicity, anti-inflammatory properties and nontumorigenicity properties for the treatment of several diseases, including replacing cells lost due to tissue injuries or degenerative diseases...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29203902/amniotic-fluid-stem-cell-derived-vesicles-protect-from-vegf-induced-endothelial-damage
#4
S Sedrakyan, V Villani, S Da Sacco, N Tripuraneni, S Porta, A Achena, M Lavarreda-Pearce, A Petrosyan, H Soloyan, R E De Filippo, B Bussolati, L Perin
Injection of amniotic fluid stem cells (AFSC) delays the course of progression of renal fibrosis in animals with Alport Syndrome, enhancing kidney function and improving survival. The mechanisms responsible for these protective outcomes are still largely unknown. Here, we showed that vascular endothelial growth factor (VEGF) signaling within the glomeruli of Alport mice is strongly elevated early on in the disease, causing glomerular endothelial cell damage. Intraventricular injected AFSC that homed within the glomeruli showed strong modulation of the VEGF activity, particularly in glomerular endothelial cells...
December 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29197837/bilateral-ocular-surface-squamous-neoplasia-with-bilateral-periocular-basal-cell-carcinoma-in-a-case-of-xeroderma-pigmentosum
#5
Rohit Agarwal, Bhavna Chawla, Mohamed Ibrahime Asif, Amar Pujari
Xeroderma pigmentosum (XP) is an autosomal recessive disorder associated with multiple oculocutaneous manifestations.We discuss a unique case of XP having bilateral ocular surface squamous neoplasia (OSSN) and periocular basal cell carcinoma. In the right eye, a large OSSN mass involving the ocular surface extensively along with intraocular invasion was noted, whereas in the left eye, the tumour mass was involving the limbus, and extending up to three clock hours. Because of extensive disease in the right eye, orbital exenteration was performed, and for the left eye, a wide excision of the mass with triple freeze thaw cryo application to the margins followed by amniotic membrane grafting was done...
December 2, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/29194396/aquaporins-during-pregnancy-their-function-and-significance
#6
REVIEW
Eszter Ducza, Adrienn Csányi, Róbert Gáspár
Water is the major component of cells and tissues, and the movement of water across the cell membrane is a fundamental property of life. Until the discovery of the first water channel, aquaporin, it was long assumed that the transport of water was due to simple diffusion through the lipid bilayer membrane that encloses cells. Aquaporin (AQP) molecules were first discovered in the human uterus in 1994, and since then several studies have investigated these channels in the female reproductive system. The expressions of AQPs have been proven in the reproductive system...
December 1, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29193858/exophiala-phaeomuriformis-keratitis-in-a-subarctic-climate-region-a-case-report
#7
André Vicente, Fátima Pedrosa Domellöf, Berit Byström
PURPOSE: To report a case of Exophiala phaeomuriformis mycotic keratitis in a patient from a subarctic climate region. Dematiaceous fungi (black yeasts) have been gaining importance as corneal keratitis and ulcer causative agents in certain regions, but no cases have been described in Scandinavia. METHODS: Case report of a patient with a persistent corneal erosion that eventually presented a brown-pigmented infiltrate. The patient had a history of several months of topical therapy comprising medication for glaucoma, corticosteroids and antibiotics...
November 29, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/29193454/amniotic-fluid-pentraxins-potential-early-markers-for-identifying-intra-amniotic-inflammatory-complications-in-preterm-pre-labor-rupture-of-membranes
#8
Ivana Musilova, Ctirad Andrys, Jan Krejsek, Marcela Drahosova, Barbora Zednikova, Lenka Pliskova, Helena Zemlickova, Bo Jacobsson, Marian Kacerovsky
In this study, pentraxin 3 (PTX3), C-reactive protein (CRP), and serum amyloid P component (SAP) concentrations in the amniotic fluid of women with preterm pre-labor rupture of membranes (PPROM) were evaluated based on evidence of microbial invasion of the amniotic cavity (MIAC), intra-amniotic inflammation (IAI), and microbial-associated IAI. A total of 149 women with PPROM were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis. Amniotic fluid PTX3, SAP, and CRP concentrations were assessed using enzyme-linked immunosorbent assay...
November 28, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29190624/extreme-surgical-maneuvers-in-fungal-endophthalmitis
#9
María Isabel Relimpio-López, María Gessa-Sorroche, Antonio Manuel Garrido-Hermosilla, Concepción Díaz-Ruiz, Jesús Montero-Iruzubieta, Jaime Etxebarría-Ecenarro, Diego Ruiz-Casas, Enrique Rodríguez-de-la-Rúa-Franch
PURPOSE: To present the different evolution of 2 cases of endophthalmitis caused by Fusarium solani, an aggressive filamentous fungus, depending on the medical and surgical treatment performed. METHODS: We present 2 cases of endophthalmitis caused by Fusarium solani. Topical, intrastromal, intravitreal, and systemic antifungal treatment (natamycin, voriconazole, amphotericin B) failed in both cases. Corneal perforation took place in one of them, being unsuccessfully treated with cyanoacrylate and several amniotic membrane transplants...
November 30, 2017: Ophthalmologica. Journal International D'ophtalmologie
https://www.readbyqxmd.com/read/29188068/32-week-premature-rupture-of-membranes-caused-by-oropharyngeal-microbiota
#10
Alberto Hidalgo-Chicharro, Raquel Abad-Torreblanca, José María Navarro-Marí, José Gutiérrez-Fernández
Introduction. Preterm premature rupture of membranes (PPROM) usually has a multifactorial etiology that is often unknown, although the most frequently reported cause is infection by group B Streptococcus. Therefore, the etiology of PPROM, although probably infectious, remains unknown in most cases. This case describes a PPROM caused by infection from oropharyngeal microbiota. Case presentation. We report the case of a 26-yr-old pregnant woman. The gestational age was 32 weeks+5 days. Examinations in the emergency department revealed the release of clear amniotic fluid and a closed multiparous cervix with a length of 22 mm...
October 2017: JMM Case Reports
https://www.readbyqxmd.com/read/29186106/late-preterm-prelabor-rupture-of-fetal-membranes-fetal-inflammatory-response-and-neonatal-outcome
#11
Ivana Musilova, Ctirad Andrys, Marcela Drahosova, Barbora Zednikova, Helena Hornychova, Lenka Pliskova, Helena Zemlickova, Bo Jacobsson, Marian Kacerovsky
OBJECTIVES: To characterize the influence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI) on the intensity of the fetal inflammatory response and the association between the presence of the fetal inflammatory response syndrome (FIRS) and short-term neonatal morbidity in the preterm prelabor rupture of membranes (PPROM) between the gestational ages of 34 and 37 weeks. METHODS: One hundred and fifty-nine women were included in the study...
November 28, 2017: Pediatric Research
https://www.readbyqxmd.com/read/29185951/intraamniotic-infection-and-the-term-neonate
#12
Deborah A Raines, Ashley Wagner, Alexander Salinas
Intraamniotic infection (IAI) may occur when the amniotic membranes are ruptured >18 hours prior to birth. The term neonate is at risk for early onset sepsis. This article describes the pathophysiology of IAI and the role of the mother-baby nurse during the anticipated birth and the ongoing assessment of the neonate.
November 1, 2017: Neonatal Network: NN
https://www.readbyqxmd.com/read/29184795/amniotic-therapeutic-biomaterials-in-urology-current-and-future-applications
#13
REVIEW
Siam Oottamasathien, James M Hotaling, James R Craig, Jeremy B Myers, William O Brant
To examine the rationale and applications of amniotic tissue augmentation in urological surgery. Published literature in English-language was reviewed for basic science and clinical use of amniotic or amnion-chorionic tissue in genitourinary tissues. Basic science and animal studies support the likely benefit of clinical applications of amnion-derived tissues in a variety of urologic interventions. The broad number of properties found in amniotic membrane, coupled with its immunologically privileged status presents a number of future applications in the urological surgical realm...
October 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/29178347/tissuepatch-is-biocompatible-and-seals-iatrogenic-membrane-defects-in-a-rabbit-model
#14
Alexander C Engels, Luc Joyeux, Johannes Van der Merwe, Julio Jimenez, Savitree Prapanus, David W Barrett, Che Connon, Tina T Chowdhury, Anna L David, Jan Deprest
OBJECTIVE: To evaluate novel sealing techniques for their biocompatibility and sealing capacity of iatrogenic fetal membrane defects in a pregnant rabbit model. METHOD: At day 23 of gestation (term=d31), a standardized fetoscopy was performed through a 14G cannula. The resulting fetal membrane defect was closed with either condensed collagen, collagen with fibrinogen, Tissuepatch®, Duraseal®, or a conventional collagen plug (Lyostypt®) as reference. At d30 the fetuses were harvested and full thickness fetal membrane samples were analyzed...
November 25, 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/29178172/anti-adhesion-therapy-following-operative-hysteroscopy-for-treatment-of-female-subfertility
#15
REVIEW
Jan Bosteels, Steven Weyers, Thomas M D'Hooghe, Helen Torrance, Frank J Broekmans, Su Jen Chua, Ben Willem J Mol
BACKGROUND: Observational evidence suggests a potential benefit with several anti-adhesion therapies in women undergoing operative hysteroscopy (e.g. insertion of an intrauterine device or balloon, hormonal treatment, barrier gels or human amniotic membrane grafting) for decreasing intrauterine adhesions (IUAs). OBJECTIVES: To assess the effectiveness of anti-adhesion therapies versus placebo, no treatment or any other anti-adhesion therapy, following operative hysteroscopy for treatment of female subfertility...
November 27, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29177171/grafting-with-cryopreserved-amniotic-membrane-versus-conservative-wound-care-in-treatment-of-pressure-ulcers-a-randomized-clinical-trial
#16
Mehdi Dehghani, Negar Azarpira, Vahid Mohammad Karimi, Hamid Mossayebi, Elaheh Esfandiari
Objective: To compare the healing process of pressure ulcers treated with cryopreserved human amniotic membrane allograft and routine pressure ulcer care in our hospital. Methods: From January 2012 to December 2013, in a prospective randomized clinical trial (IRCT201612041335N2), 24 patients with second and third stage of pressure ulcers were enrolled in this study. All patients needed split-thickness skin grafts for pressure ulcer-wound coverage. Selected patients had symmetric ulcers on both upper and lower extremities...
October 2017: Bulletin of Emergency and Trauma
https://www.readbyqxmd.com/read/29176767/h1n1-influenza-virus-infection-results-in-adverse-pregnancy-outcomes-by-disrupting-tissue-specific-hormonal-regulation
#17
Elizabeth Q Littauer, E Stein Esser, Olivia Q Antao, Elena V Vassilieva, Richard W Compans, Ioanna Skountzou
Increased susceptibility to influenza virus infection during pregnancy has been attributed to immunological changes occurring before and during gestation in order to "tolerate" the developing fetus. These systemic changes are most often characterized by a suppression of cell-mediated immunity and elevation of humoral immune responses referred to as the Th1-Th2 shift. However, the underlying mechanisms which increase pregnant mothers' risk following influenza virus infection have not been fully elucidated. We used pregnant BALB/c mice during mid- to late gestation to determine the impact of a sub-lethal infection with A/Brisbane/59/07 H1N1 seasonal influenza virus on completion of gestation...
November 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29176451/comparison-of-pterygium-recurrence-rates-among-different-races-and-ethnicities-after-primary-pterygium-excision-by-surgeons-in-training
#18
Giovanni Campagna, Matthew Adams, Li Wang, Sumitra Khandelwal, Zaina Al-Mohtaseb
PURPOSE: To evaluate the impact of race and ethnicity, surgical technique, and level of surgeon training on recurrence rates after primary pterygium excision. METHODS: A retrospective review of patients who underwent primary pterygium excision at our academic institution was performed. The surgical technique-conjunctival autografting (CAG) or amniotic membrane grafting (AMG)-was chosen at the attending surgeon's discretion, and all surgeries were performed by surgeons in training...
November 22, 2017: Cornea
https://www.readbyqxmd.com/read/29171370/-ocular-involvement-in-stevens-johnson-syndrome-treatment-with-amniotic-membrane-transplantation-in-the-acute-phase
#19
S H Kardaun, K Huei Liem, R Stoutenbeek
Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are both part of a spectrum of serious mucocutaneous disorders, most often caused by drugs, with a high morbidity and mortality. In the acute stage, serious skin and mucocutaneous lesions with painful blistering, erosions and systemic involvement present the main focus of attention. The severity of skin manifestations in the acute stage, however, does not necessarily correlate with that of the mucosal lesions. Most feared are long-term sequelae, especially chronic eye involvement, which can be highly disabling...
2017: Nederlands Tijdschrift Voor Geneeskunde
https://www.readbyqxmd.com/read/29167357/how-fish-eggs-are-preadapted-for-the-evolution-of-matrotrophy
#20
Keenan R Morrison, Vyvian Ngo, Richard A Cardullo, David N Reznick
Teleost fishes evolved livebearing via egg retention 14 times. Matrotrophy has evolved within 12 of those lineages. By contrast, squamate reptiles evolved livebearing over 115 times, but only two to four of those lineages are known to have evolved matrotrophy. Is the discrepancy between these organisms in the probability of this transition caused by differences in their eggs? We show that the eggs of oviparous species in the superorder Atherinomorpha can acquire small organic molecules from their surrounding environment against a concentration gradient via mechanisms of active transport...
November 29, 2017: Proceedings. Biological Sciences
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