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Amniotic membran

Chareenun Chirapapaisan, Pinnita Prabhasawat, Sabong Srivannaboon, Vatookarn Roongpoovapatr, Piyada Chitsuthipakorn
Purpose: We report a rare case of ocular injury due to potassium permanganate (KMnO4 ) granules in a child. Methods: This is a retrospective case report. Results: A 2-year-old boy was transferred to our emergency room with severe pain in his right eye, inflamed eyelids, and brownish stains on his fingers. Chemical injury was suspected. Copious eye irrigation was immediately performed. Diffuse brownish splotches were then observed at the inferior bulbar conjunctiva...
January 2018: Case Reports in Ophthalmology
Tomoko Sato, Koji Sugioka, Aya Kodama-Takahashi, Masahiko Fukuda, Hiroshi Mishima, Yoshikazu Shimomura
Purpose: To report a case of neurotrophic keratopathy associated with nasopharyngeal carcinoma. Case Report: A 59-year-old man who had been diagnosed with a nasopharyngeal carcinoma was referred to the authors because of visual disturbance and pain in his right eye. Slit-lamp examination revealed a corneal epithelial defect and corneal stromal edema surrounding the epithelial defect area in his right eye. Magnetic resonance imaging showed a mass in his cavernous sinus, which was identified as nasopharyngeal carcinoma (NPC)...
January 2018: Case Reports in Ophthalmology
Yi-Chen Sun, Jason P Kam, Tueng T Shen
Objective: Appropriate management of nontraumatic acute corneal perforation is always a challenge even with the many advances in surgical materials and techniques. We reported the outcomes of a case series of acute corneal perforation repair using a newly modified conjunctival flap with amniotic membrane transplant (AMT), fibrin glue, and a bandage soft contact lens (BCL). Materials and Methods: A total of 13 consecutive referral cases with nontraumatic acute corneal perforation at the University of Washington were reviewed...
January 2018: Ci Ji Yi Xue za Zhi, Tzu-chi Medical Journal
Hui Luo, Ailan Xie, Ying Hua, Jing Wang, Yanyun Liu, Xueqiong Zhu
OBJECTIVE: To explore the role of aquaporin 1 (AQP1) in regulation of amniotic fluid volume and composition. To investigate the effects of AQP1 gene knockout on expression of other aquaporin water channels (AQP3, AQP8 and AQP9) in placentas and fetal membranes. METHODS: Mice were sacrificed at 9.5, 13.5 and 16.5 gestational day (GD). Amniotic fluid volume, osmolality and composition, fetal membranes, placental and fetal weights as well as placenta areas were recorded in AQP1 homozygote conceptus group, heterozygote conceptus group and wild-type group, respectively...
April 4, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Aurélien Tidu, Djida Ghoubay-Benallaoua, Claire Teulon, Sophie Asnacios, Kate Grieve, François Portier, Marie-Claire Schanne-Klein, Vincent Borderie, Gervaise Mosser
This study aimed at controlling both the organization and the transparency of dense collagen scaffolds making use of the lyotropic mesogen properties of collagen. Cholesteric or plywood-like liquid crystal phases were achieved using mixtures of acetic and hydrochloric acids as solvents. The critical pH at which the switch between the two phases occurred was around pH = 3. The use of the two acids led to fibrillated collagen I scaffolds, whose visual aspect ranged from opaque to transparent. Rheological investigations showed that viscoelastic properties of the plywood-like solutions were optimized for molding due to faster recovery...
April 6, 2018: Biomaterials Science
Malina Wagner, Peter Walter, Sabine Salla, Sandra Johnen, Niklas Plange, Stephan Rütten, Tamme W Goecke, Matthias Fuest
PURPOSE: Amniotic membrane (AM) is an essential tool in ocular surface reconstruction. In this study, we analyzed the differential effects of glycerol and straight storage at - 80 °C for up to 6 months on the structural, biological, and mechanical properties of amniotic membrane (AM). METHODS: Human placentae of 11 different subjects were analyzed. AMs were stored at - 80 °C, either with a 1:1 mixture of Dulbecco's modified Eagle medium and glycerol (glycerol) or without any medium or additives (straight)...
April 5, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
Young-Eun Cho, Byoung-Joon Song, Mohammed Akbar, Moon-Chang Baek
Extracellular vesicles (EVs) are small membranous vesicles originating from various cells and tissues, including the liver parenchymal hepatocytes and nonparenchymal cells such as Kupffer and stellate cells. Recently, the pathophysiological role of EVs, such as exosomes and microvesicles, has been increasingly recognized based on intercellular communications. These EVs travel through the circulating blood and interact with specific cells and then deliver their cargos such as nucleic acids and proteins into recipient cells...
April 3, 2018: Pharmacology & Therapeutics
A B Araújo, J M Furlan, G D Salton, T Schmalfuss, L M Röhsig, L M R Silla, E P Passos, A H Paz
OBJECTIVE: To compare four enzymatic protocols for mesenchymal stem cells (MSCs) isolation from amniotic (A-MSC) and chorionic (C-MSC) membranes, umbilical cord (UC-MSC) and placental decidua (D-MSC) in order to define a robust, practical and low-cost protocol for each tissue. RESULTS: A-MSCs and UC-MSCs could be isolated from all samples using trypsin/collagenase-based protocols; C-MSCs could be isolated from all samples with collagenase- and trypsin/collagenase-based protocols; D-MSCs were isolated from all samples exclusively with a collagenase-based protocol...
April 4, 2018: Biotechnology Letters
Huanhuan Liu, Zhengbing Zhou, Hui Lin, Juan Wu, Brian Ginn, Ji Suk Choi, Xuesong Jiang, Liam Chung, Jennifer H Elisseeff, Samuel Yiu, Hai-Quan Mao
Severe damage to ocular surface can result in limbal stem cell (LSC) deficiency, which contributes to loss of corneal clarity, potential vision loss, chronic pain, photophobia, and keratoplasty failure. Human amniotic membrane (AM) is the most effective substrate for LSC transplantation to treat patients with LSC deficiency. However, the widespread use of the AM in the clinic remains a challenge due to the high cost for preserving freshly prepared AMs and the weak mechanical strength of lyophilized AM. Here we developed a novel composite membrane consisting of an electrospun bioabsorbable polymer fiber mesh bonded to a decellularized AM (dAM) sheet through interfacial conjugation...
April 3, 2018: ACS Applied Materials & Interfaces
Ahmed M Maged, Ali M El-Semary, Heba M Marie, Doaa S Belal, Ayman Hany, Mohammad A Taymour, Eman F Omran, Sahar M Y Elbaradie, Mohamed A Kamal Mohamed
OBJECTIVE: To test the hypothesis that there is a higher rate of unsuccessful induction of labor (IOL) in post-term obese pregnant women compared to non-obese ones. METHODS: In this prospective cohort study, 144 obese (BMI > 30) and 144 non-obese (BMI < 29.9) post-term (> 41 weeks) pregnant women were recruited. IOL was done by misoprostol or amniotomy and oxytocin infusion according to the Bishop score. Comparison of percentage of failed IOL in both groups (primary outcome) was performed by the Chi-test...
April 2, 2018: Archives of Gynecology and Obstetrics
Jing Li, Xiaohui Luo, Hongmin Ke, Lingyi Liang
Atopic keratoconjunctivitis (AKC) is the most severe type of allergic conjunctivitis and may eventually lead to blindness. Although AKC is reported to be more prevalent in adults, we report a child with AKC whose clinical characteristics were not inconsistent with those typically seen in adult patients with AKC, and who was refractory to traditional topical anti-inflammatory and immunosuppressant therapies. An 11-year-old boy presented with a 3-month history of ocular redness and itching and decreased vision for a week in both eyes...
April 2018: Pediatrics
Soheil Koushaei, Mohammad Hassan Samandari, Sayed Mohammad Razavi, Ahad Khoshzaban, Shahriar Adibi, Payam Varedi
The purpose of this article was to evaluate the bone induction effects of an amnion membrane protected graft compared with collagen membrane protected graft in repair of tibial bony defects in dogs. This study was performed using the tibial bone of dogs. After the removal of periostium, similar holes were made with a 16 mm trephine drill (38 holes in total). For the study group ten holes were covered by absorbable collagen and 16 holes by amniotic membrane. In control group 12 holes were made and were covered by the overlying soft tissue...
April 2, 2018: Journal of Oral Implantology
Tejsu Malla, Jing Jiang, Kai Hu
AIM: To compare long-term outcome of primary and recurrent pterygium surgery with three different techniques: combined conjunctival autograft and overlay amniotic membrane transplantation (CAT with AMT), conjunctival autograft transplantation (CAT) alone and amniotic membrane transplantation (AMT) alone. METHODS: In this retrospective study, 142 eyes of 142 pterygium patients (104 primary, 38 recurrent) who underwent CAT (group A), AMT (group B) or CAT with AMT (group C) respectively following surgical excision were reviewed and compared based on the recurrences and post-operative complications...
2018: International Journal of Ophthalmology
Matthias Becker, Janita A Maring, Maria Schneider, Aarón X Herrera Martin, Martina Seifert, Oliver Klein, Thorsten Braun, Volkmar Falk, Christof Stamm
There is a growing need for scaffold material with tissue-specific bioactivity for use in regenerative medicine, tissue engineering, and for surgical repair of structural defects. We developed a novel composite biomaterial by processing human cardiac extracellular matrix (ECM) into a hydrogel and combining it with cell-free amniotic membrane via a dry-coating procedure. Cardiac biocompatibility and immunogenicity were tested in vitro using human cardiac fibroblasts, epicardial progenitor cells, murine HL-1 cells, and human immune cells derived from buffy coat...
March 29, 2018: International Journal of Molecular Sciences
G Chisari, E M Chisari, A M Borzì, A Grasso, C G Chisari
INTRODUCTION: Skin lesions can be defined as lesions that result in loss of tissues and their joints, and often this cutaneous skin process is a primary or secondary consequence of the structural changes in the skin itself. Subjects with peripheral arteripathies that develop chronic skin lesions in the lower extremities of the Western world are constantly increasing. We conducted a study on the etiologic incidence of chronic skin lesions in peripheral arterial disease CSLpa subjects in the lower limbs compared to subjects with chronic skin lesions CSL (controls)...
March 2018: La Clinica Terapeutica
Cecilia Y Cheung, Debra F Anderson, Marion Rouzaire, Loïc Blanchon, Vincent Sapin, Robert A Brace
Vascular endothelial growth factor (VEGF) has been proposed as an important regulator of amniotic fluid absorption across the amnion into the fetal vasculature on the surface of the placenta. However, the activators of VEGF expression and action in the amnion have not been identified. Using the pregnant sheep model, we aimed to investigate the presence of the retinoic acid (RA) pathway in ovine amnion and to determine its effect on VEGF expression. Further, we explored relationships between RA receptors and VEGF and tested the hypothesis that RA modulates intramembranous absorption (IMA) through induction of amnion VEGF in sheep fetuses subjected to altered IMA rates...
January 1, 2018: Reproductive Sciences
Peter Trosan, Ingrida Smeringaiova, Kristyna Brejchova, Jan Bednar, Oldrich Benada, Olga Kofronova, Katerina Jirsova
The human amniotic membrane (HAM) is widely used for its wound healing effect in clinical practice, as a feeder for the cell cultivation, or a source of cells to be used in cell therapy. The aim of this study was to find effective and safe enzymatic HAM de-epithelialization method leading to harvesting of both denuded undamaged HAM and viable human amniotic epithelial cells (hAECs). The efficiency of de-epithelialization using TrypLE Express, trypsin/ ethylenediaminetetraacetic (EDTA), and thermolysin was monitored by hematoxylin and eosin staining and by the measurement of DNA concentration...
2018: PloS One
Paula Laranjeira, Marta Duque, Martin Vojtek, Maria J Inácio, Isabel Silva, Ana C Mamede, Mafalda Laranjo, Susana Pedreiro, Maria J Carvalho, Paulo Moura, Ana M Abrantes, Cláudio J Maia, Pedro Domingues, Rosário Domingues, António Martinho, Maria F Botelho, Hélder Trindade, Artur Paiva
The discovery of the immunoregulatory potential of human amniotic membrane (hAM) propelled several studies focusing on its application for the treatment of immunological disorders. However, there is little information regarding the effects of hAM on distinct activation and differentiation stages of immune cells. Here, we aim to investigate the effect of human amniotic membrane extract (hAME) on the pattern of cytokine production by T cells, monocytes and myeloid dendritic cells (mDCs). For this purpose, peripheral blood mononuclear cells (PBMCs) from eight healthy individuals were stimulated in vitro in the presence or absence of hAME...
March 26, 2018: Cell and Tissue Research
Barbara Bolis, Paola Scarpa, Alessandro Rota, Tiziana Vitiello, Maria Cristina Veronesi
In order to define the normal composition of canine amniotic fluid and to detect differences between surviving and non-surviving newborn puppies, the present study determined the uric acid, glucose, lactate and creatinine concentrations and the lactate to creatinine ratio in amniotic fluids collected during elective Caesarean section from small-sized purebred bitches. The possible relationship between newborn survival and the studied parameters, as well as the effects of maternal parity, fetal gender and Apgar score were assessed...
March 2018: Acta Veterinaria Hungarica
Melis Palamar, Banu Yaman, Taner Akalın, Ayse Yağcı
Objectives: To investigate the long-term efficacy and results of surgical management of conjunctival melanoma reconstructed with amniotic membrane transplantation. Materials and Methods: Conjunctival melanoma in 10 patients (5 female, 5 male) was totally excised with adjunctive cryotherapy to the surgical margins, corneal epitheliectomy with absolute alcohol in cases of corneal involvement, lamellar sclerectomy in cases with episcleral involvement, and ocular surface grafting with cryopreserved amniotic membrane...
February 2018: Turkish Journal of Ophthalmology
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