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Amniotic membran

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https://www.readbyqxmd.com/read/28317126/pharmacokinetics-of-ceftiofur-sodium-in-equine-pregnancy
#1
M L Macpherson, S Giguère, M A Pozor, E Runcan, T W Vickroy, S A Benson, M H T Troedsson, J N Hatzel, J Larson, E Vanden Berg, A A Kelleman, L C Sanchez, M M LeBlanc
Eleven pregnant pony mares (D270-326) were administered ceftiofur sodium intramuscularly at 2.2 mg/kg (n = 6) or 4.4 mg/kg (n = 5), once daily. Plasma was obtained prior to ceftiofur administration and at 0.5, 1, 2, 4, 8, 12, and 24 hr after administration. Eight pony mares were re-enrolled in the study at least 3 days from expected foaling to ensure steady-state concentrations of drug at the time of foaling. Mares were administered ceftiofur sodium (4.4 mg/kg, IM) daily until foaling. Parturition was induced using oxytocin 1 hr after ceftiofur sodium administration...
March 19, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28316944/human-amnion-membrane-potential-applications-in-oral-and-periodontal-field
#2
REVIEW
Ranjana Mohan, Aashima Bajaj, Mohan Gundappa
Human amniotic membrane (HAM) is derived from the fetal membranes which consist of the inner amniotic membrane made of single layer of amnion cells fixed to collagen-rich mesenchyme attached to chorion. HAM has low immunogenicity, anti-inflammatory properties and their cells can be isolated without the sacrifice of human embryos. Amniotic membrane has biological properties which are important for the experimental and clinical applications in managing patients of various medical specialties. Abundant, natural and wonderful biomembrane not only protects the foetus but also has various clinical applications in the field of dermatology, ophthalmology, ENT surgery, orthopedics and dental surgery...
January 2017: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/28316190/-allogeneic-cultured-limbal-epithelial-transplantation-a-clinical-observation-of-its-treatment-effects-on-large-conjunctival-lesions
#3
J Cheng, J Y Wang, H Y Duan, S Wang, L Cong, L X Xie
Objective: To introduce the surgical methods and investigate the treatment effects of conjunctival lesion resection combined with transplantation of allogeneic limbal epithelia cultured in vitro for treatment of varied large conjunctival lesions. Methods: A retrospective analysis of case data of the patients receiving transplantation of allogeneic limbal epithelia cultured in vitro for large conjunctival lesions at Qingdao Eye Hospital from January 2013 to December 2015 was conducted. Fresh donor corneal limbal epithelial tissues were obtained for cellular digestion and isolation...
March 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28300874/amniotic-membrane-as-an-option-for-treatment-of-acute-achilles-tendon-injury-in-rats
#4
Mariana de Castro Nicodemo, Lia Renó das Neves, Josafá Carvalho Aguiar, Flaviane de Souza Brito, Isabelle Ferreira, Luciana Barros Sant'Anna, Leandro José Raniero, Rodrigo Álvaro Lopes Martins, Paulo Roxo Barja, Emilia Angela Lo Schiavo Arisawa
PURPOSE: To evaluate the effect of human amniotic membrane (hAM) fragment on inflammatory response, proliferation of fibroblast and organization of collagen fibers in injured tendon. METHODS: Sixty rats were divided into 3 groups: C - surgical procedures without tendon lesion and with simulation of hAM application; I - surgical procedures, tendon injury and simulation of hAM application; T - surgical procedures, tendon injury and hAM application. These groups were subdivided into four experimental times (3, 7, 14 and 28 days)...
February 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28293501/management-of-a-complex-excoriation-disorder-induced-wound-with-a-viable-cryopreserved-placental-membrane
#5
Michael A Bain, Jennifer Vincent
Excoriation disorder (ED), also known as dermatotillomania, is a condition characterized by repeated "skin picking" that leads to the formation of skin lesions. Because of the similarity of its symptoms to obsessive compulsive disorder, ED is classified as a subcategory of obsessive compulsive disorder by Diagnostic and Statistical Manual of Mental Disorders Fifth Edition. Although the majority of the self-inflicted wounds are not clinically significant, many wounds lead to social and occupational dysfunction by becoming infected, chronic, and life threatening...
December 2016: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/28289611/comparison-of-characteristics-of-human-amniotic-membrane-and-human-adipose-tissue-derived-mesenchymal-stem-cells
#6
Khadijeh Dizaji Asl, Hajar Shafaei, Jafar Soleimani Rad, Hojjat Ollah Nozad
BACKGROUND: Mesenchymal stem cells (MSCs) are ideal candidates for treatment of diseases. Amniotic membranes are an inexpensive source of MSCs (AM-MSC) without any donor site morbidity in cell therapy. Adipose tissue derived stem cells (ASCs) are also suitable cells for cell therapy. There is discrepancy in CD271 expression among MSCs from different sources. In this study, the characteristics of AM-MSC and ASCs and CD271 expression were compared. METHODS: Adult adipose tissue samples were obtained from patients undergoing elective surgical procedure, and samples of amniotic membrane were collected immediately after caesarean operation...
January 2017: World Journal of Plastic Surgery
https://www.readbyqxmd.com/read/28288269/topical-cryopreserved-amniotic-membrane-and-umbilical-cord-eye-drops-promote-re-epithelialization-in-a-murine-corneal-abrasion-model
#7
Sean Tighe, Hamid-Reza Moein, Lorraine Chua, Anny Cheng, Pedram Hamrah, Scheffer C G Tseng
Purpose: To evaluate morselized amniotic membrane and umbilical cord (AMUC) eye drops in promoting corneal re-epithelialization. Methods: Following a 2-mm diameter central epithelial wound in one eye of 48 normal C57BL/6 mouse corneas, 10 μL of saline with (n = 24) or without (n = 24) AMUC was applied three times a day for 6 days. The corneal epithelial defect was measured using 0.1% fluorescein, while corneal epithelial regularity was measured by assessment of a reflected light off the corneal surface...
March 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28286810/epidemiology-and-related-risk-factors-of-preterm-labor-as-an-obstetrics-emergency
#8
Ali Asghar Halimi Asl, Saeed Safari, Mohsen Parvareshi Hamrah
INTRODUCTION: Preterm birth is still a major health problem throughout the world, which results in 75% of neonatal mortality. Preterm labor not only inflicts financial and emotional distress, it may also lead to permanent disability. The present study was conducted to determine the related risk factors and preventive measures of preterm labor. METHODS: This retrospective cross-sectional study assessed all preterm labors, as well as an equal number of term labors, during seven years, at an educational hospital...
2017: Emergency (Tehran, Iran)
https://www.readbyqxmd.com/read/28286306/a-controlled-study-of-the-role-of-cryopreserved-amniotic-membrane-transplant-during-strabismus-reoperations
#9
Rehab Rashad Kassem, Ahmed Mostafa Kamal, Randa Mohamed Abdel-Moneim El-Mofty, Hala Mostafa Elhilali
PURPOSE: To evaluate the effects of using cryopreserved human amniotic membrane (AM) transplant during strabismus reoperations. METHODS: A total of 30 patients with persistent strabismus were included in this controlled, prospective study. Patients were divided into two groups of 15 patients each. The AM group underwent strabismus reoperation with wrapping of the muscles with cryopreserved amniotic membrane. Controls underwent strabismus reoperation without an AM wrap...
March 9, 2017: Journal of AAPOS: the Official Publication of the American Association for Pediatric Ophthalmology and Strabismus
https://www.readbyqxmd.com/read/28286051/vasa-previa-with-an-intact-amniotic-membrane
#10
Shinya Matsuzaki, Masayuki Endo, Tadashi Kimura
This extremely rare image shows the positional relationship of the cord vessel, amniotic membrane, and cervical os during pregnancy.
March 9, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28278303/clinical-significance-of-histologic-chorioamnionitis-with-a-negative-amniotic-fluid-culture-in-patients-with-preterm-labor-and-premature-membrane-rupture
#11
Jeong Woo Park, Kyo Hoon Park, Eun Young Jung
OBJECTIVE: To evaluate the effect of histological chorioamnionitis (HCA) with a negative amniotic fluid (AF) culture on adverse pregnancy and neonatal outcomes and inflammatory status in the AF compartment in women with preterm labor or preterm premature rupture of membranes (PPROM). METHODS: This is a retrospective cohort study of 153 women diagnosed as having a preterm labor or PPROM (20-34 weeks) who delivered singleton gestations within 48 hours of amniocentesis...
2017: PloS One
https://www.readbyqxmd.com/read/28277919/cervical-fluid-interleukin-6-and-intra-amniotic-complications-of-preterm-prelabor-rupture-of-membranes
#12
Ivana Musilova, Ctirad Andrys, Marcela Drahosova, Ondrej Soucek, Lenka Pliskova, Bo Jacobsson, Marian Kacerovsky
OBJECTIVE: To determine if cervical fluid interleukin (IL)-6 concentrations in women with preterm prelabor rupture of membranes (PPROM) allows identification of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). METHODS: One hundred forty-four women with singleton pregnancies complicated by PPROM were included in this prospective cohort study. Cervical and amniotic fluids were collected at the time of admission and concentrations of IL-6 were measured using an ELISA and point-of-care test, respectively...
February 21, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28267444/a-multicenter-prospective-study-of-neonatal-outcomes-at-less-than-32-weeks-associated-with-indications-for-maternal-admission-and-delivery
#13
Thomas J Garite, C Andrew Combs, Kimberly Maurel, Anita Das, Kevin Huls, Richard Porreco, Dale Reisner, George Lu, Melissa Bush, Bruce Morris, April Bleich
BACKGROUND: Counseling for patients with impending premature delivery has been traditionally based primarily on the projected gestational age at delivery. There are limited data regarding how the indications for the preterm birth affect the neonatal outcome and whether this issue should be taken into account in decisions regarding management and patient counseling. OBJECTIVE: We performed a prospective study of pregnancies resulting in premature delivery at less than 32 weeks to determine the influence of both the Indications for Admission and their associated Indications for Delivery on neonatal mortality and complications of prematurity...
March 3, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28259958/comparison-of-the-proliferation-migration-and-angiogenic-properties-of-human-amniotic-epithelial-and-mesenchymal-stem-cells-and-their-effects-on-endothelial-cells
#14
Qianqian Wu, Tao Fang, Hongxin Lang, Min Chen, Ping Shi, Guoxian Qi, Xining Pang
In vivo studies have shown that amnion-produced growth factors participate in many diseases that involve angiogenesis, re-epithelialization and immunomodulation. Although human amniotic epithelial cells (hAECs) and human amniotic mesenchymal stem cells (hAMSCs) can be obtained from amniotic membranes, there is little information regarding their biological differences. The aim of the present study was to isolate and characterize cells from human amnions, to investigate the biological potential and behavior of these cells on the function of endothelial cells in vivo and in vitro and to examine variations in the expression profile of growth factors in different human amnion-derived cell types...
February 20, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28258575/aquaporins-in-fetal-development
#15
Nora Martínez, Alicia E Damiano
Water homeostasis during fetal development is of crucial physiologic importance. The successful formation and development of the placenta is critical to maintain normal fetal growth and homeostasis. The expression of several aquaporins (AQPs ) was found from blastocyst stages to term placenta and fetal membranes. Therefore, AQPs are proposed to play important roles in normal pregnancy, fetal growth, and homeostasis of amniotic fluid volume, and water handling in other organs. However, the functional importance of AQPs in fetal development remains to be elucidated...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28257385/in-vitro-evaluation-of-the-drug-reservoir-function-of-human-amniotic-membrane-using-moxifloxacin-as-a-model-drug
#16
Madhavi Latha Yelchuri, Bhagyashree Madhavi, Nilam Gohil, Hitha Sara Sajeev, Namperumalsamy Venkatesh Prajna, Senthilkumari Srinivasan
PURPOSE: To evaluate the in vitro, extended drug reservoir function of human amniotic membrane (HAM) of different thicknesses impregnated with moxifloxacin. METHODS: HAM buttons (12 mm) were soaked with freshly prepared 0.5% wt/vol topical moxifloxacin at different soaking time intervals: 3 hours (group I), 6 hours (group II), 12 hours (group III), 24 hours (group IV), and 48 hours (group V). They were then transferred into 1 mL of fresh simulated tear fluid (pH-7...
March 1, 2017: Cornea
https://www.readbyqxmd.com/read/28255771/amniotic-membrane-in-ophthalmology-properties-preparation-storage-and-indications-for-grafting-a-review
#17
REVIEW
Katerina Jirsova, Gary L A Jones
The use of amniotic membrane in ophthalmic surgery and other surgical procedures in the fields of dermatology, plastic surgery, genitourinary medicine and otolaryngology is on the increase. Furthermore, amniotic membrane and its epithelial and mesenchymal cells have broad use in regenerative medicine and hold great promise in anticancer treatment. Amniotic membrane is a rich source of biologically active factors and as such, promotes healing and acts as an effective material for wound dressing. Amniotic membrane supports epithelialization and exhibits anti-fibrotic, anti-inflammatory, anti-angiogenic and anti-microbial features...
March 2, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28252047/inhibition-of-proliferation-and-epithelial-mesenchymal-transition-in-retinal-pigment-epithelial-cells-by-heavy-chain-hyaluronan-pentraxin-3
#18
Hua He, Ajay E Kuriyan, Chen-Wei Su, Megha Mahabole, Yuan Zhang, Ying-Ting Zhu, Harry W Flynn, Jean-Marie Parel, Scheffer C G Tseng
Proliferative vitreoretinopathy (PVR) is mediated by proliferation and epithelial mesenchymal transition (EMT) of retinal pigment epithelium (RPE). Because heavy chain-hyaluronic acid/pentraxin 3 (HC-HA/PTX3) purified from human amniotic membrane exerts anti-inflammatory and anti-scarring actions, we hypothesized that HC-HA/PTX3 could inhibit these PVR-related processes in vitro. In this study, we first optimized an ARPE-19 cell culture model to mimic PVR by defining cell density, growth factors, and cultivation time...
March 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28239777/-limbal-epithelial-stem-cell-transplantation-current-state-and-perspectives
#19
D Meller, H Thomasen
Homeostasis of the corneal surface is maintained by epithelial stem cells localized in the limbus. Multiple intrinsic factors or external injuries can destroy the delicate microenvironment of limbal epithelial stem cells causing a state which is termed limbal stem cell deficiency (LSCD). In such cases, re-epithelialization of the cornea is drastically impeded and conjunctival epithelium starts to extend beyond the limbus and to invade the corneal surface. In partial LSCD, a superficial keratectomy combined with an amniotic membrane is advised and helpful to restore an intact, healthy ocular surface...
February 26, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/28237988/transabdominal-amniocentesis-is-a-feasible-and-safe-procedure-in-preterm-prelabor-rupture-of-membranes
#20
Ivana Musilova, Tomas Bestvina, Jaroslav Stranik, Martin Stepan, Bo Jacobsson, Marian Kacerovsky
OBJECTIVES: To determine the feasibility and the complication rate of amniocentesis in a large cohort of women with preterm prelabor rupture of membranes (PPROM). METHODS: A retrospective cohort study was conducted in all women with singleton gestation complicated by PPROM at between 24+0 and 36+6 weeks admitted to the Department of Obstetrics and Gynecology, University Hospital Hradec Kralove, Czech Republic between May 2008 and July 2016. Amniocentesis was offered as a part of a routine protocol of PPROM for the detection of microbial invasion of the amniotic cavity and intra-amniotic inflammation...
February 25, 2017: Fetal Diagnosis and Therapy
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