Kyubin Seo, Subin Cho, Hyogeun Shin, Aeri Shin, Ju-Hyun Lee, June Hoan Kim, Boram Lee, Hwanseok Jang, Youngju Kim, Hyo Min Cho, Yongdoo Park, Hee Youn Kim, Taeseob Lee, Woong-Yang Park, Yong Jun Kim, Esther Yang, Dongho Geum, Hyun Kim, Il-Joo Cho, Sanghyuk Lee, Jae Ryun Ryu, Woong Sun
Axis formation and related spatial patterning are initiated by symmetry breaking during development. A geometrically confined culture of human pluripotent stem cells (hPSCs) mimics symmetry breaking and cell patterning. Using this, polarized spinal cord organoids (pSCOs) with a self-organized dorsoventral (DV) organization are generated. The application of caudalization signals promoted regionalized cell differentiation along the radial axis and protrusion morphogenesis in confined hPSC colonies. These detached colonies grew into extended spinal cord-like organoids, which established self-ordered DV patterning along the long axis through the spontaneous expression of polarized DV patterning morphogens...
May 12, 2023: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)