keyword
MENU ▼
Read by QxMD icon Read
search

microcontact printing

keyword
https://www.readbyqxmd.com/read/29144124/ionic-liquid-microstrips-impregnated-with-magnetic-nano-stirrers-for-sensitive-gas-sensors
#1
Richard Gondosiswanto, David Brynn Hibbert, Yu Fang, Chuan Zhao
Ionic liquids have been regarded as promising electrolytes as substitutes for volatile aqueous or organic solvents for electrochemical gas sensors. However ionic liquids are viscous and the slow diffusion of gas molecules leads to poor sensitivity and sluggish response times. Herein, we describe a strategy using an array of microstrips of ionic liquid containing magnetic nanoparticles as nano-stirrers for enhanced mass transport and gas sensing. Magnetic CoFe2O4 nanoparticles are synthesized and dispersed in a hydrophobic ionic liquid [BMP][Ntf2]...
November 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29140078/molecular-recognition-and-immobilization-of-ligand-conjugated-redox-responsive-polymer-nanocontainers
#2
Wilke De Vries, Matthias Tesch, Armido Studer, Bart Jan Ravoo
We present the preparation of ligand conjugated redox-responsive polymer nanocontainers by the supramolecular decoration of cyclodextrin vesicles with a thin redox-cleavable polymer shell that displays molecular recognition units on its surface. Two widely different recognition motifs (mannose-Concanavalin A and biotin-streptavidin) are compared and the impact of ligand density is studied using aggregation assays, dynamic light scattering, and a fluorometric quantification of ligands. Microcontact printing is used to prepare streptavidin-patterned surfaces and the specific immobilization of biotin conjugated nanocontainers is demonstrated...
November 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29120467/electrophoretic-cytometry-of-adherent-cells
#3
Elaine J Su, Amy E Herr
Cell-matrix and cell-cell interactions influence intracellular signalling and play an important role in physiologic and pathologic processes. Detachment of cells from the surrounding microenvironment alters intracellular signalling. Here, we demonstrate and characterise an integrated microfluidic device to culture single and clustered cells in tuneable microenvironments and then directly analyse the lysate of each cell in situ, thereby eliminating the need to detach cells prior to analysis. First, we utilise microcontact printing to pattern cells in confined geometries...
November 9, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28893070/astrocyte-spreading-and-migration-on-aggrecan-laminin-dot-gradients
#4
Tony W Hsiao, Patrick A Tresco, Vladimir Hlady
The surface concentration gradient of two extracellular matrix (ECM) macromolecules was developed to study the migratory and morphological responses of astrocytes to molecular cues typically found in the central nervous system injury environment. The gradient, prepared using microcontact printing, was composed of randomly positioned micrometer-sized dots of aggrecan (AGG) printed on a substrate uniformly coated with laminin (LN). AGG dots were printed in an increasing number along the 1000 μm long and 50 μm wide gradient area which had on each end either a full surface coverage of AGG or LN...
September 11, 2017: Biointerphases
https://www.readbyqxmd.com/read/28842903/microcontact-printed-hydrogel-microwell-arrays-for-clonal-muscle-stem-cell-cultures
#5
Victor M Aguilar, Benjamin D Cosgrove
Adult muscle stem cells (also called satellite cells) are an anatomically defined population of cells that are essential for muscle regeneration. In aging and dystrophic diseases, muscle stem cells (MuSCs) exhibit functional and molecular heterogeneity; therefore, single-cell assay technologies are critical to illuminate the mechanisms of pathological stem cell dysfunction. Here, we describe the process of generating mechanically tunable hydrogels with micro-scale well features ("microwells") using micro-contact printing for clonal muscle stem cell culture assays...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28776977/diffractive-protein-gratings-as-optically-active-transducers-for-high-throughput-label-free-immunosensing
#6
Miquel Avella-Oliver, Javier Carrascosa, Rosa Puchades, Ángel Maquieira
A novel label-free biosensing approach based on bioreceptor networks patterned as diffractive gratings (biogratings) has been developed. Nanogrooved structures were used as optically active scaffolds for producing arrays of functional BSA biogratings on low energy surfaces by a water-assisted variant of microcontact printing. An analytical scanner, comprising a LightScribe compact disk drive, was developed to measure the diffraction patterns of these biogratings, thus allowing biointeractions to be quantitatively sensed in a multiplex and label-free fashion by means of diffraction efficiency changes...
September 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28766344/cell-shaping-micropatterns-for-quantitative-super-resolution-microscopy-imaging-of-membrane-mechanosensing-proteins
#7
Anthony Fernandez, Markville Bautista, Ramunas Stanciauskas, Taerin Chung, Fabien Pinaud
Patterning cells on microcontact-printed substrates is a powerful approach to control cell morphology and introduce specific mechanical cues on a cell's molecular organization. Although global changes in cellular architectures caused by micropatterns can easily be probed with diffraction-limited optical microscopy, studying molecular reorganizations at the nanoscale demands micropatterned substrates that accommodate the optical requirements of single molecule microscopy techniques. Here, we developed a simple micropatterning strategy that provides control of cellular architectures and is optimized for nanometer accuracy single molecule tracking and three-dimensional super-resolution imaging of plasma and nuclear membrane proteins in cells...
August 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28760620/microcontact-printing-of-polydopamine-on-thermally-expandable-hydrogels-for-controlled-cell-adhesion-and-delivery-of-geometrically-defined-microtissues
#8
Yu Bin Lee, Se-Jeong Kim, Eum Mi Kim, Hayeon Byun, Hyung-Kwan Chang, Jungyul Park, Yu Suk Choi, Heungsoo Shin
Scaffold-free harvest of microtissue with a defined structure has received a great deal of interest in cell-based assay and regenerative medicine. In this study, we developed thermally expandable hydrogels with spatially controlled cell adhesive patterns for rapid harvest of geometrically controlled microtissue. We patterned polydopamine (PD) on to the hydrogel via microcontact printing (μCP), in linear shapes with widths of 50, 100 and 200μm. The hydrogels facilitated formation of spatially controlled strip-like microtissue of human dermal fibroblasts (HDFBs)...
July 28, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28733449/mitochondrial-function-in-engineered-cardiac-tissues-is-regulated-by-extracellular-matrix-elasticity-and-tissue-alignment
#9
Davi M Lyra-Leite, Allen M Andres, Andrew P Petersen, Nethika R Ariyasinghe, Nathan Cho, Jezell A Lee, Roberta A Gottlieb, Megan L McCain
Mitochondria in cardiac myocytes are critical for generating ATP to meet the high metabolic demands associated with sarcomere shortening. Distinct remodeling of mitochondrial structure and function occur in cardiac myocytes in both developmental and pathological settings. However, the factors that underlie these changes are poorly understood. Because remodeling of tissue architecture and extracellular matrix (ECM) elasticity are also hallmarks of ventricular development and disease, we hypothesize that these environmental factors regulate mitochondrial function in cardiac myocytes...
October 1, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28650173/polymer-pen-lithography-with-lipids-for-large-area-gradient-patterns
#10
Ravi Kumar, Ainhoa Urtizberea, Souvik Ghosh, Uwe Bog, Quinn Rainer, Steven Lenhert, Harald Fuchs, Michael Hirtz
Gradient patterns comprising bioactive compounds over comparably (in regard to a cell size) large areas are key for many applications in the biomedical sector, in particular, for cell screening assays, guidance, and migration experiments. Polymer pen lithography (PPL) as an inherent highly parallel and large area technique has a great potential to serve in the fabrication of such patterns. We present strategies for the printing of functional phospholipid patterns via PPL that provide tunable feature size and feature density gradients over surface areas of several square millimeters...
July 6, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28627756/microcontact-peeling-a-cell-micropatterning-technique-for-circumventing-direct-adsorption-of-proteins-to-hydrophobic-pdms
#11
Sho Yokoyama, Tsubasa S Matsui, Shinji Deguchi
Microcontact printing (μCPr) is one of the most popular techniques used for cell micropatterning. In conventional μCPr, a polydimethylsiloxane (PDMS) stamp with microfeatures is used to adsorb extracellular matrix (ECM) proteins onto the featured surface and transfer them onto particular areas of a cell culture substrate. However, some types of functional proteins other than ECM have been reported to denature upon direct adsorption to hydrophobic PDMS. Here we describe a detailed protocol of an alternative technique--microcontact peeling (μCPe)--that allows for cell micropatterning while circumventing the step of adsorbing proteins to bare PDMS...
June 19, 2017: Current Protocols in Cell Biology
https://www.readbyqxmd.com/read/28590253/when-lithography-meets-self-assembly-a-review-of-recent-advances-in-the-directed-assembly-of-complex-metal-nanostructures-on-planar-and-textured-surfaces
#12
Robert Hughes, Eredzhep Menumerov, Svetlana Neretina
One of the foremost challenges in nanofabrication is the establishment of a processing science that integrates wafer-based materials, techniques, and devices with the extraordinary physicochemical properties accessible when materials are reduced to nanoscale dimensions. Such a merger would allow for exacting controls on nanostructure positioning, promote cooperative phenomenon between adjacent nanostructures and/or substrate materials, and allow for electrical contact to individual or groups of nanostructures...
June 7, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28540951/on-surface-o-glycosylation-by-catalytic-microcontact-printing
#13
Moritz Buhl, Serena Traboni, Martin Körsgen, Sebastian Lamping, Heinrich F Arlinghaus, Bart Jan Ravoo
The generation of carbohydrate patterns on surfaces enables a wide range of analytical and diagnostic applications and efficient methods for carbohydrate immobilization are crucial for this purpose. We report on surface O-glycosylation by catalytic printing as a novel, effective method for the covalent immobilization of carbohydrates in micropatterns. Beside the verification of surface functionalization, the suitability of the generated surface for ligand protein interactions was demonstrated.
June 6, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28513733/polylactic-acid-nano-and-microchamber-arrays-for-encapsulation-of-small-hydrophilic-molecules-featuring-drug-release-via-high-intensity-focused-ultrasound
#14
Meiyu Gai, Johannes Frueh, Tianyi Tao, Arseniy V Petrov, Vladimir V Petrov, Evgeniy V Shesterikov, Sergei I Tverdokhlebov, Gleb B Sukhorukov
Long term encapsulation combined with spatiotemporal release for a precisely defined quantity of small hydrophilic molecules on demand remains a challenge in various fields ranging from medical drug delivery, controlled release of catalysts to industrial anti-corrosion systems. Free-standing individually sealed polylactic acid (PLA) nano- and microchamber arrays were produced by one-step dip-coating a PDMS stamp into PLA solution for 5 s followed by drying under ambient conditions. The wall thickness of these hydrophobic nano-microchambers is tunable from 150 nm to 7 μm by varying the PLA solution concentration...
June 1, 2017: Nanoscale
https://www.readbyqxmd.com/read/28488588/leaf-inspired-microcontact-printing-vascular-patterns
#15
Lian Wong, John Pegan, Basia Gabela-Zuniga, Michelle Khine, Kara E McCloskey
The vascularization of tissue grafts is critical for maintaining viability of the cells within a transplanted graft. A number of strategies are currently being investigated including very promising microfluidics systems. Here, we explored the potential for generating a vasculature-patterned endothelial cells (EC) that could be integrated into distinct layers between sheets of primary cells. Bioinspired from the leaf veins, we generated a reverse mold with a fractal vascular-branching pattern that models the unique spatial arrangement over multiple length scales that precisely mimic branching vasculature...
May 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28452456/polylactic-acid-sealed-polyelectrolyte-multilayer-microchambers-for-entrapment-of-salts-and-small-hydrophilic-molecules-precipitates
#16
Meiyu Gai, Johannes Frueh, Valeriya L Kudryavtseva, Alexey M Yashchenok, Gleb B Sukhorukov
Efficient depot systems for entrapment and storage of small water-soluble molecules are of high demand for wide variety of applications ranging from implant based drug delivery in medicine and catalysis in chemical processes to anticorrosive systems in industry where surface-mediated active component delivery is required on a time and site specific manner. This work reports the fabrication of individually sealed hollow-structured polyelectrolyte multilayer (PEM) microchamber arrays based on layer-by-layer self-assembly as scaffolds and microcontact printing...
May 5, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28430279/microcontact-printing-with-aminosilanes-creating-biomolecule-micro-and-nanoarrays-for-multiplexed-microfluidic-bioassays
#17
Shivani Sathish, Sébastien G Ricoult, Kazumi Toda-Peters, Amy Q Shen
Microfluidic systems integrated with protein and DNA micro- and nanoarrays have been the most sought-after technologies to satisfy the growing demand for high-throughput disease diagnostics. As the sensitivity of these systems relies on the bio-functionalities of the patterned recognition biomolecules, the primary concern has been to develop simple technologies that enable biomolecule immobilization within microfluidic devices whilst preserving bio-functionalities. To address this concern, we introduce a two-step patterning approach to create micro- and nanoarrays of biomolecules within microfluidic devices...
April 21, 2017: Analyst
https://www.readbyqxmd.com/read/28406198/topological-defects-in-epithelia-govern-cell-death-and-extrusion
#18
LETTER
Thuan Beng Saw, Amin Doostmohammadi, Vincent Nier, Leyla Kocgozlu, Sumesh Thampi, Yusuke Toyama, Philippe Marcq, Chwee Teck Lim, Julia M Yeomans, Benoit Ladoux
Epithelial tissues (epithelia) remove excess cells through extrusion, preventing the accumulation of unnecessary or pathological cells. The extrusion process can be triggered by apoptotic signalling, oncogenic transformation and overcrowding of cells. Despite the important linkage of cell extrusion to developmental, homeostatic and pathological processes such as cancer metastasis, its underlying mechanism and connections to the intrinsic mechanics of the epithelium are largely unexplored. We approach this problem by modelling the epithelium as an active nematic liquid crystal (that has a long range directional order), and comparing numerical simulations to strain rate and stress measurements within monolayers of MDCK (Madin Darby canine kidney) cells...
April 12, 2017: Nature
https://www.readbyqxmd.com/read/28380036/simple-agarose-micro-confinement-array-and-machine-learning-based-classification-for-analyzing-the-patterned-differentiation-of-mesenchymal-stem-cells
#19
Nobuyuki Tanaka, Tadahiro Yamashita, Asako Sato, Viola Vogel, Yo Tanaka
The geometrical confinement of small cell colonies gives differential cues to cells sitting at the periphery versus the core. To utilize this effect, for example to create spatially graded differentiation patterns of human mesenchymal stem cells (hMSCs) in vitro or to investigate underpinning mechanisms, the confinement needs to be robust for extended time periods. To create highly repeatable micro-fabricated structures for cellular patterning and high-throughput data mining, we employed here a simple casting method to fabricate more than 800 adhesive patches confined by agarose micro-walls...
2017: PloS One
https://www.readbyqxmd.com/read/28285531/copper-contamination-of-self-assembled-organic-monolayer-modified-silicon-surfaces-following-a-click-reaction-characterized-with-laps-and-spim
#20
Fan Wu, De-Wen Zhang, Jian Wang, Michael Watkinson, Steffi Krause
A copper(I)-catalyzed azide alkyne cycloaddition (CuAAC) reaction combined with microcontact printing was used successfully to pattern alkyne-terminated self-assembled organic monolayer-modified silicon surfaces. Despite the absence of a copper peak in X-ray photoelectron spectra, copper contamination was found and visualized using light-addressable potentiometric sensors (LAPS) and scanning photo-induced impedance microscopy (SPIM) after the "click"-modified silicon surfaces were rinsed with hydrochloric acid (HCl) solution, which was frequently used to remove copper residues in the past...
March 24, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
keyword
keyword
54875
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"