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https://www.readbyqxmd.com/read/29334733/surface-functionalization-with-carboxylic-acids-by-photochemical-microcontact-printing-and-tetrazole-chemistry
#1
Christoph Buten, Sebastian Lamping, Martin Körsgen, Heinrich F Arlinghaus, Craig Jamieson, Bart Jan Ravoo
In this paper we show that carboxylic acid functionalized molecules can be patterned by photochemical microcontact printing on tetrazole-terminated self-assembled monolayers. Upon irradiation, tetrazoles eliminate nitrogen to form highly reactive nitrile imines, which can be ligated with several different nucleophiles, carboxylic acids being most reactive. As a proof of concept, we immobilized trifluoroacetic acid to monitor the reaction with X-ray photoelectron spectroscopy (XPS). Moreover, we also immobilized peptides and fabricated carbohydrate-lectin as well as biotin-streptavidin microarrays using this method...
January 16, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29304548/protein-substrate-adhesion-in-microcontact-printing-regulates-cell-behaviors
#2
Shuhuan Hu, Ting-Hsuan Chen, Yanhua Zhao, Zuankai Wang, Raymond H W Lam
Microcontact printing (μCP) is widely used to create patterns of biomolecules essential for studies of cell mechanics, migration, and tissue engineering. However, different types of μCPs may create micropatterns with varied protein-substrate adhesion, which may change cell behaviors and pose uncertainty in result interpretation. Here, we characterize two μCP methods for coating extracellular matrix (ECM) proteins (stamp-off and covalent-bond) and demonstrate for the first time the important role of protein-substrate adhesion in determining cell behaviors...
January 5, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29298336/controlling-cell-shape-on-hydrogels-using-lift-off-protein-patterning
#3
Jens Moeller, Aleksandra K Denisin, Joo Yong Sim, Robin E Wilson, Alexandre J S Ribeiro, Beth L Pruitt
Polyacrylamide gels functionalized with extracellular matrix proteins are commonly used as cell culture platforms to evaluate the combined effects of extracellular matrix composition, cell geometry and substrate rigidity on cell physiology. For this purpose, protein transfer onto the surface of polyacrylamide hydrogels must result in geometrically well-resolved micropatterns with homogeneous protein distribution. Yet the outcomes of micropatterning methods have not been pairwise evaluated against these criteria...
2018: PloS One
https://www.readbyqxmd.com/read/29236477/plasma-assisted-large-scale-nanoassembly-of-metal-insulator-bioplasmonic-mushrooms
#4
Nikhil Bhalla, Shivani Sathish, Casey James Galvin, Robert Anthony Campbell, Abhishek Sinha, Amy Q Shen
Large-scale plasmonic substrates consisting of metal-insulator nanostructures coated with a bio-recognition layer can be exploited for enhanced label-free sensing, by utilizing the principle of localized surface plasmon resonance (LSPR). Most often the uniformity and thickness of the bio-recognition layer determines the sensitivity of plasmonic resonances as the inherent LSPR sensitivity of nanomaterials is limited to 10-20 nm from the surface. However, due to time-consuming nanofabrication processes, there is limited work on both the development of large-scale plasmonic materials, and the subsequent surface functionalizing with bio-recognition layers...
December 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29225769/fabrication-of-low-cost-micropatterned-polydimethyl-siloxane-scaffolds-to-organise-cells-in-a-variety-of-two-dimensioanl-biomimetic-arrangements-for-lab-on-chip-culture-platforms
#5
Lidia Escutia-Guadarrama, Genaro Vázquez-Victorio, David Martínez-Pastor, Brenda Nieto-Rivera, Marcela Sosa-Garrocho, Marina Macías-Silva, Mathieu Hautefeuille
We present the rapid-prototyping of type I collagen micropatterns on poly-dimethylsiloxane substrates for the biomimetic confinement of cells using the combination of a surface oxidation treatment and 3-aminopropyl triethoxysilane silanisation followed by glutaraldehyde crosslinking. The aim of surface treatment is to stabilise microcontact printing transfer of this natural extracellular matrix protein that usually wears out easily from poly-dimethylsiloxane, which is not suitable for biomimetic cell culture platforms and lab-on-chip applications...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29221371/two-step-nanoscale-approach-for-well-defined-complex-alkanethiol-films-on-au-surfaces
#6
Shova Neupane, Patricia Losada-Pérez, Sebastien Vivegnis, Zineb Mekhalif, Joseph Delhalle, Asif Bashir, Frank Uwe U Renner
Controlling the molecular organization of organic self-assembled monolayers (SAM) is of utmost importance in nanotechnology, molecular electronics, and surface science. Here we propose two well-differentiated approaches, double printing based on microcontact printing (µ-cp) and molecular back-filling adsorption, to produce complex alkanethiol films. The resulting films on model Au surfaces were characterized by atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements...
December 8, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29144124/ionic-liquid-microstrips-impregnated-with-magnetic-nano-stirrers-for-sensitive-gas-sensors
#7
Richard Gondosiswanto, David Brynn Hibbert, Yu Fang, Chuan Zhao
Ionic liquids have been regarded as promising electrolytes as substitutes for volatile aqueous or organic solvents for electrochemical gas sensors. However ionic liquids are viscous and the slow diffusion of gas molecules leads to poor sensitivity and sluggish response times. Herein, we describe a strategy using an array of microstrips of ionic liquid containing magnetic nanoparticles as nano-stirrers for enhanced mass transport and gas sensing. Magnetic CoFe2O4 nanoparticles are synthesized and dispersed in a hydrophobic ionic liquid [BMP][Ntf2]...
November 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29140078/molecular-recognition-and-immobilization-of-ligand-conjugated-redox-responsive-polymer-nanocontainers
#8
Wilke De Vries, Matthias Tesch, Armido Studer, Bart Jan Ravoo
We present the preparation of ligand conjugated redox-responsive polymer nanocontainers by the supramolecular decoration of cyclodextrin vesicles with a thin redox-cleavable polymer shell that displays molecular recognition units on its surface. Two widely different recognition motifs (mannose-Concanavalin A and biotin-streptavidin) are compared and the impact of ligand density is studied using aggregation assays, dynamic light scattering, and a fluorometric quantification of ligands. Microcontact printing is used to prepare streptavidin-patterned surfaces and the specific immobilization of biotin conjugated nanocontainers is demonstrated...
November 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29120467/electrophoretic-cytometry-of-adherent-cells
#9
Elaine J Su, Amy E Herr
Cell-matrix and cell-cell interactions influence intracellular signalling and play an important role in physiologic and pathologic processes. Detachment of cells from the surrounding microenvironment alters intracellular signalling. Here, we demonstrate and characterise an integrated microfluidic device to culture single and clustered cells in tuneable microenvironments and then directly analyse the lysate of each cell in situ, thereby eliminating the need to detach cells prior to analysis. First, we utilise microcontact printing to pattern cells in confined geometries...
December 5, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28893070/astrocyte-spreading-and-migration-on-aggrecan-laminin-dot-gradients
#10
Tony W Hsiao, Patrick A Tresco, Vladimir Hlady
The surface concentration gradient of two extracellular matrix (ECM) macromolecules was developed to study the migratory and morphological responses of astrocytes to molecular cues typically found in the central nervous system injury environment. The gradient, prepared using microcontact printing, was composed of randomly positioned micrometer-sized dots of aggrecan (AGG) printed on a substrate uniformly coated with laminin (LN). AGG dots were printed in an increasing number along the 1000 μm long and 50 μm wide gradient area which had on each end either a full surface coverage of AGG or LN...
September 11, 2017: Biointerphases
https://www.readbyqxmd.com/read/28842903/microcontact-printed-hydrogel-microwell-arrays-for-clonal-muscle-stem-cell-cultures
#11
Victor M Aguilar, Benjamin D Cosgrove
Adult muscle stem cells (also called satellite cells) are an anatomically defined population of cells that are essential for muscle regeneration. In aging and dystrophic diseases, muscle stem cells (MuSCs) exhibit functional and molecular heterogeneity; therefore, single-cell assay technologies are critical to illuminate the mechanisms of pathological stem cell dysfunction. Here, we describe the process of generating mechanically tunable hydrogels with micro-scale well features ("microwells") using micro-contact printing for clonal muscle stem cell culture assays...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28776977/diffractive-protein-gratings-as-optically-active-transducers-for-high-throughput-label-free-immunosensing
#12
Miquel Avella-Oliver, Javier Carrascosa, Rosa Puchades, Ángel Maquieira
A novel label-free biosensing approach based on bioreceptor networks patterned as diffractive gratings (biogratings) has been developed. Nanogrooved structures were used as optically active scaffolds for producing arrays of functional BSA biogratings on low energy surfaces by a water-assisted variant of microcontact printing. An analytical scanner, comprising a LightScribe compact disk drive, was developed to measure the diffraction patterns of these biogratings, thus allowing biointeractions to be quantitatively sensed in a multiplex and label-free fashion by means of diffraction efficiency changes...
September 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28766344/cell-shaping-micropatterns-for-quantitative-super-resolution-microscopy-imaging-of-membrane-mechanosensing-proteins
#13
Anthony Fernandez, Markville Bautista, Ramunas Stanciauskas, Taerin Chung, Fabien Pinaud
Patterning cells on microcontact-printed substrates is a powerful approach to control cell morphology and introduce specific mechanical cues on a cell's molecular organization. Although global changes in cellular architectures caused by micropatterns can easily be probed with diffraction-limited optical microscopy, studying molecular reorganizations at the nanoscale demands micropatterned substrates that accommodate the optical requirements of single molecule microscopy techniques. Here, we developed a simple micropatterning strategy that provides control of cellular architectures and is optimized for nanometer accuracy single molecule tracking and three-dimensional super-resolution imaging of plasma and nuclear membrane proteins in cells...
August 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28760620/microcontact-printing-of-polydopamine-on-thermally-expandable-hydrogels-for-controlled-cell-adhesion-and-delivery-of-geometrically-defined-microtissues
#14
Yu Bin Lee, Se-Jeong Kim, Eum Mi Kim, Hayeon Byun, Hyung-Kwan Chang, Jungyul Park, Yu Suk Choi, Heungsoo Shin
Scaffold-free harvest of microtissue with a defined structure has received a great deal of interest in cell-based assay and regenerative medicine. In this study, we developed thermally expandable hydrogels with spatially controlled cell adhesive patterns for rapid harvest of geometrically controlled microtissue. We patterned polydopamine (PD) on to the hydrogel via microcontact printing (μCP), in linear shapes with widths of 50, 100 and 200μm. The hydrogels facilitated formation of spatially controlled strip-like microtissue of human dermal fibroblasts (HDFBs)...
July 28, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28733449/mitochondrial-function-in-engineered-cardiac-tissues-is-regulated-by-extracellular-matrix-elasticity-and-tissue-alignment
#15
Davi M Lyra-Leite, Allen M Andres, Andrew P Petersen, Nethika R Ariyasinghe, Nathan Cho, Jezell A Lee, Roberta A Gottlieb, Megan L McCain
Mitochondria in cardiac myocytes are critical for generating ATP to meet the high metabolic demands associated with sarcomere shortening. Distinct remodeling of mitochondrial structure and function occur in cardiac myocytes in both developmental and pathological settings. However, the factors that underlie these changes are poorly understood. Because remodeling of tissue architecture and extracellular matrix (ECM) elasticity are also hallmarks of ventricular development and disease, we hypothesize that these environmental factors regulate mitochondrial function in cardiac myocytes...
October 1, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28650173/polymer-pen-lithography-with-lipids-for-large-area-gradient-patterns
#16
Ravi Kumar, Ainhoa Urtizberea, Souvik Ghosh, Uwe Bog, Quinn Rainer, Steven Lenhert, Harald Fuchs, Michael Hirtz
Gradient patterns comprising bioactive compounds over comparably (in regard to a cell size) large areas are key for many applications in the biomedical sector, in particular, for cell screening assays, guidance, and migration experiments. Polymer pen lithography (PPL) as an inherent highly parallel and large area technique has a great potential to serve in the fabrication of such patterns. We present strategies for the printing of functional phospholipid patterns via PPL that provide tunable feature size and feature density gradients over surface areas of several square millimeters...
July 6, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28627756/microcontact-peeling-a-cell-micropatterning-technique-for-circumventing-direct-adsorption-of-proteins-to-hydrophobic-pdms
#17
Sho Yokoyama, Tsubasa S Matsui, Shinji Deguchi
Microcontact printing (μCPr) is one of the most popular techniques used for cell micropatterning. In conventional μCPr, a polydimethylsiloxane (PDMS) stamp with microfeatures is used to adsorb extracellular matrix (ECM) proteins onto the featured surface and transfer them onto particular areas of a cell culture substrate. However, some types of functional proteins other than ECM have been reported to denature upon direct adsorption to hydrophobic PDMS. Here we describe a detailed protocol of an alternative technique--microcontact peeling (μCPe)--that allows for cell micropatterning while circumventing the step of adsorbing proteins to bare PDMS...
June 19, 2017: Current Protocols in Cell Biology
https://www.readbyqxmd.com/read/28590253/when-lithography-meets-self-assembly-a-review-of-recent-advances-in-the-directed-assembly-of-complex-metal-nanostructures-on-planar-and-textured-surfaces
#18
Robert Hughes, Eredzhep Menumerov, Svetlana Neretina
One of the foremost challenges in nanofabrication is the establishment of a processing science that integrates wafer-based materials, techniques, and devices with the extraordinary physicochemical properties accessible when materials are reduced to nanoscale dimensions. Such a merger would allow for exacting controls on nanostructure positioning, promote cooperative phenomenon between adjacent nanostructures and/or substrate materials, and allow for electrical contact to individual or groups of nanostructures...
June 7, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28540951/on-surface-o-glycosylation-by-catalytic-microcontact-printing
#19
Moritz Buhl, Serena Traboni, Martin Körsgen, Sebastian Lamping, Heinrich F Arlinghaus, Bart Jan Ravoo
The generation of carbohydrate patterns on surfaces enables a wide range of analytical and diagnostic applications and efficient methods for carbohydrate immobilization are crucial for this purpose. We report on surface O-glycosylation by catalytic printing as a novel, effective method for the covalent immobilization of carbohydrates in micropatterns. Beside the verification of surface functionalization, the suitability of the generated surface for ligand protein interactions was demonstrated.
June 6, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28513733/polylactic-acid-nano-and-microchamber-arrays-for-encapsulation-of-small-hydrophilic-molecules-featuring-drug-release-via-high-intensity-focused-ultrasound
#20
Meiyu Gai, Johannes Frueh, Tianyi Tao, Arseniy V Petrov, Vladimir V Petrov, Evgeniy V Shesterikov, Sergei I Tverdokhlebov, Gleb B Sukhorukov
Long term encapsulation combined with spatiotemporal release for a precisely defined quantity of small hydrophilic molecules on demand remains a challenge in various fields ranging from medical drug delivery, controlled release of catalysts to industrial anti-corrosion systems. Free-standing individually sealed polylactic acid (PLA) nano- and microchamber arrays were produced by one-step dip-coating a PDMS stamp into PLA solution for 5 s followed by drying under ambient conditions. The wall thickness of these hydrophobic nano-microchambers is tunable from 150 nm to 7 μm by varying the PLA solution concentration...
June 1, 2017: Nanoscale
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