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https://www.readbyqxmd.com/read/28045220/long-term-stable-poly-acrylamide-brush-modified-transparent-microwells-for-cell-attachment-studies-in-3d
#1
Inga Lilge, Siyu Jiang, Holger Schönherr
The fabrication of 3D cell microenvironments exploiting versatile, long-term stable passivating poly(acryl amide) brushes in a microwell format and the study of the behavior of fibroblast and pancreatic tumor cells in wells of systematically varied shape and size is reported. The microwells, which are obtained by combining micromolding in capillaries with microcontact printing of initiator monolayers for subsequent surface-initiated polymerization of acrylamide and controlled functionalization with fibronectin (FN), expose cell adhesive areas inside the wells and protein and cell resistant brushes on the topside plateaus...
January 3, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28044285/capillary-driven-microfluidic-chips-for-miniaturized-immunoassays-patterning-capture-antibodies-using-microcontact-printing-and-dry-film-resists
#2
Yuksel Temiz, Robert D Lovchik, Emmanuel Delamarche
The miniaturization of immunoassays using microfluidic devices is attractive for many applications, but an important challenge remains the patterning of capture antibodies (cAbs) on the surface of microfluidic structures. Here, we describe how to pattern cAbs on planar poly(dimethylsiloxane) (PDMS) stamps and how to microcontact print the cAbs on a dry-film resist (DFR). DFRs are new types of photoresists having excellent chemical resistance and good mechanical, adhesive, and optical properties. Instead of being liquid photoresists, DFRs are thin layers that are easy to handle, cut, photo-pattern, and laminate over surfaces...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27991750/fabrication-of-patterned-thermoresponsive-microgel-strips-on-cell-adherent-background-and-their-application-for-cell-sheet-recovery
#3
Yongqing Xia, Ying Tang, Han Wu, Jing Zhang, Zongyi Li, Fang Pan, Shengjie Wang, Xiaojuan Wang, Hai Xu, Jian Ren Lu
Interfaces between materials and cells play a critical role in cell biomedical applications. Here, a simple, robust, and cost-effective method is developed to fabricate patterned thermoresponsive poly(N-isopropylacrylamide-co-styrene) microgel strips on a polyethyleneimine-precoated, non-thermoresponsive cell-adherent glass coverslip. The aim is to investigate whether cell sheets could be harvested from these cell-adherent surfaces patterned with thermoresponsive strips comprised of the microgels. We hypothesize that if the cell-to-cell interaction is strong enough to retain the whole cell sheet from disintegration, the cell segments growing on the thermoresponsive strips may drag the cell segments growing on the cell-adherent gaps to detach, ending with a whole freestanding and transferable cell sheet...
January 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27982514/stretchable-substrates-for-the-assembly-of-polymeric-microstructures
#4
T P Vinod, Jay M Taylor, Abhiteja Konda, Stephen A Morin
The directed assembly of micro-/nanoscale objects relies on physical or chemical processes to generate structures that are not possible via self-assembly alone. A relatively unexplored strategy in directed assembly is the "active" manipulation of building blocks through deformations of elastomeric substrates. This manuscript reports a method which uses macroscopic mechanical deformations of chemically modified silicone films to realize the rational assembly of microscopic polymer structures. Specifically, polystyrene microparticles are deposited onto polydimethylsiloxane substrates using microcontact-printing where, through a process that involved stretching/relaxing the substrates and bonding of the particles, they are elaborated into microstructures of various sizes, shapes, symmetries, periodicities, and functionalities...
December 16, 2016: Small
https://www.readbyqxmd.com/read/27830831/patterned-microstructure-fabrication-polyelectrolyte-complexes-vs-polyelectrolyte-multilayers
#5
Meiyu Gai, Johannes Frueh, Valeriya L Kudryavtseva, Rui Mao, Maxim V Kiryukhin, Gleb B Sukhorukov
Polyelectrolyte complexes (PEC) are formed by mixing the solutions of oppositely charged polyelectrolytes, which were hitherto deemed "impossible" to process, since they are infusible and brittle when dry. Here, we describe the process of fabricating free-standing micro-patterned PEC films containing array of hollow or filled microchambers by one-step casting with small applied pressure and a PDMS mould. These structures are compared with polyelectrolyte multilayers (PEM) thin films having array of hollow microchambers produced from a layer-by-layer self-assembly of the same polyelectrolytes on the same PDMS moulds...
November 10, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27797131/preparation-of-functional-alternating-polymer-brushes-and-their-orthogonal-surface-modification-through-microcontact-printing
#6
Moritz Buhl, Matthias Tesch, Sebastian Lamping, Johanna Moratz, Armido Studer, Bart Jan Ravoo
This paper reports microcontact printing (μCP) to immobilize an alkoxyamine initiator (regulator) on glass and silicon substrates and subsequent surface-initiated alternating nitroxide-mediated copolymerization (siNMP) of hexafluoroisopropyl acrylate (HFIPA) and 7-octenylvinyl ether (OVE). The resulting patterned polymer brushes are analyzed by using atomic force microscopy (AFM). In addition, site-specific post-functionalization of the alternating polymer brushes by applying two orthogonal surface reactions is achieved with thiols and amines through μCP...
October 31, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27782398/microcontact-printing-of-thiol-functionalized-ionic-liquid-microarrays-for-membrane-less-and-spill-less-gas-sensors
#7
Richard Gondosiswanto, Christian A Gunawan, David B Hibbert, Jason B Harper, Chuan Zhao
Lab-on-a-chip systems have gained significant interest for both chemical synthesis and assays at the micro-to-nanoscale with a unique set of benefits. However, solvent volatility represents one of the major hurdles to the reliability and reproducibility of the lab-on-a-chip devices for large-scale applications. Here we demonstrate a strategy of combining nonvolatile and functionalized ionic liquids with microcontact printing for fabrication of "wall-less" microreactors and microfluidics with high reproducibility and high throughput...
November 16, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27700106/micropatterning-of-phase-segregated-supported-lipid-bilayers-and-binary-lipid-phases-through-polymer-stencil-lift-off
#8
Yujie Zhu, Jose M Moran-Mirabal
Supported lipid bilayers (SLBs) provide an excellent model system for studying structural and functional characteristics of biomembranes. Patterning model membranes on solid supports has elicited much interest because lipid bilayer arrays at cellular or sub-cellular scales provide attractive platforms for reconstituting tissue-like conditions for cell culture, and for creating simplified physiological environments to study biological processes. Phase-segregated SLB patterns can be especially useful for such studies, as the selective functionalization of the lipid phases with different lipids, receptors or proteins can be achieved to mimic the key features of plasma membrane...
October 4, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27681742/automated-and-multiplexed-soft-lithography-for-the-production-of-low-density-dna-microarrays
#9
Julie Fredonnet, Julie Foncy, Jean-Christophe Cau, Childérick Séverac, Jean Marie François, Emmanuelle Trévisiol
Microarrays are established research tools for genotyping, expression profiling, or molecular diagnostics in which DNA molecules are precisely addressed to the surface of a solid support. This study assesses the fabrication of low-density oligonucleotide arrays using an automated microcontact printing device, the InnoStamp 40(®). This automate allows a multiplexed deposition of oligoprobes on a functionalized surface by the use of a MacroStamp(TM) bearing 64 individual pillars each mounted with 50 circular micropatterns (spots) of 160 µm diameter at 320 µm pitch...
2016: Microarrays
https://www.readbyqxmd.com/read/27611362/microcontact-printing-in-bioanalysis-where-are-we-and-where-shall-we-be
#10
Rituraj Dubey, Ravi Bhushan
No abstract text is available yet for this article.
October 2016: Bioanalysis
https://www.readbyqxmd.com/read/27511952/human-neuron-cultures-micropatterning-facilitates-the-long-term-growth-and-analysis-of-ipsc-derived-individual-human-neurons-and-neuronal-networks-adv-healthcare-mater-15-2016
#11
Lena F Burbulla, Kristin G Beaumont, Milan Mrksich, Dimitri Krainc
Dimitri Krainc, Milan Mrksich, and co-workers demonstrate the utility of microcontact printing technology for culturing of human neurons in defined patterns over extended periods of time on page 1894. This approach facilitates studies of neuronal development, cellular trafficking, and related mechanisms that require assessment of individual neurons and neuronal networks.
August 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27379230/signal-propagation-between-neuronal-populations-controlled-by-micropatterning
#12
Jonas Albers, Andreas Offenhäusser
The central nervous system consists of an unfathomable number of functional networks enabling highly sophisticated information processing. Guided neuronal growth with a well-defined connectivity and accompanying polarity is essential for the formation of these networks. To investigate how two-dimensional protein patterns influence neuronal outgrowth with respect to connectivity and functional polarity between adjacent populations of neurons, a microstructured model system was established. Exclusive cell growth on patterned substrates was achieved by transferring a mixture of poly-l-lysine and laminin to a cell-repellent glass surface by microcontact printing...
2016: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/27351136/heparin-micropatterning-onto-fouling-release-perfluoropolyether-based-polymers-via-photobiotin-activation
#13
Caterina Credi, Carmela De Marco, Elena Molena, Mateu Pla Roca, Josep Samitier Martí, Joana Marques, Xavier Fernàndez-Busquets, Marinella Levi, Stefano Turri
A simple method for constructing versatile ordered biotin/avidin arrays on UV-curable perfluoropolyethers (PFPEs) is presented. The goal is the realization of a versatile platform where any biotinylated biological ligands can be further linked to the underlying biotin/avidin array. To this end, microcontact arrayer and microcontact printing technologies were developed for photobiotin direct printing on PFPEs. As attested by fluorescence images, we demonstrate that this photoactive form of biotin is capable of grafting onto PFPEs surfaces during irradiation...
October 1, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27350122/prolonged-culture-of-aligned-skeletal-myotubes-on-micromolded-gelatin-hydrogels
#14
Archana Bettadapur, Gio C Suh, Nicholas A Geisse, Evelyn R Wang, Clara Hua, Holly A Huber, Alyssa A Viscio, Joon Young Kim, Julie B Strickland, Megan L McCain
In vitro models of skeletal muscle are critically needed to elucidate disease mechanisms, identify therapeutic targets, and test drugs pre-clinically. However, culturing skeletal muscle has been challenging due to myotube delamination from synthetic culture substrates approximately one week after initiating differentiation from myoblasts. In this study, we successfully maintained aligned skeletal myotubes differentiated from C2C12 mouse skeletal myoblasts for three weeks by utilizing micromolded (μmolded) gelatin hydrogels as culture substrates, which we thoroughly characterized using atomic force microscopy (AFM)...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27157272/direct-and-reversible-immobilization-and-microcontact-printing-of-functional-proteins-on-glass-using-a-genetically-appended-silica-binding-tag
#15
Brandon L Coyle, François Baneyx
Fusion of disulfide-constrained or linear versions of the Car9 dodecapeptide to model fluorescent proteins support their on-contact and oriented immobilization onto unmodified glass. Bound proteins can be released and the surface regenerated by incubation with l-lysine. This noncovalent chemistry enables rapid and reversibe microcontact printing of tagged proteins and speeds up the production of bicontinuous protein patterns.
May 19, 2016: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/27151936/facile-and-high-throughput-synthesis-of-functional-microparticles-with-quick-response-codes
#16
Lisa Marie S Ramirez, Muhan He, Shay Mailloux, Justin George, Jun Wang
Encoded microparticles are high demand in multiplexed assays and labeling. However, the current methods for the synthesis and coding of microparticles either lack robustness and reliability, or possess limited coding capacity. Here, a massive coding of dissociated elements (MiCODE) technology based on innovation of a chemically reactive off-stoichimetry thiol-allyl photocurable polymer and standard lithography to produce a large number of quick response (QR) code microparticles is introduced. The coding process is performed by photobleaching the QR code patterns on microparticles when fluorophores are incorporated into the prepolymer formulation...
June 2016: Small
https://www.readbyqxmd.com/read/27122113/field-effect-transistors-ultrathin-mxene-micropattern-based-field-effect-transistor-for-probing-neural-activity-adv-mater-17-2016
#17
Bingzhe Xu, Minshen Zhu, Wencong Zhang, Xu Zhen, Zengxia Pei, Qi Xue, Chunyi Zhi, Peng Shi
A field-effect transistor (FET) based on ultrathin Ti3 C2 -MXene micropatterns is developed by C. Zhi, P. Shi, and co-workers, as described on page 3333. The FET can be utilized for label-free probing of small molecules in typical biological environments, e.g., for fast detection of action potentials in primary neurons. This device is produced with a microcontact printing technique, harnessing the unique advantages for easy fabrication.
May 2016: Advanced Materials
https://www.readbyqxmd.com/read/27121296/compressive-broad-band-hyperspectral-sum-frequency-generation-microscopy-to-study-functionalized-surfaces
#18
Desheng Zheng, Liyang Lu, Yun Li, Kevin F Kelly, Steven Baldelli
A broad-band sum frequency generation microscope has been developed for the study of molecular monolayers on surfaces. Because sum frequency generation is a vibrational spectroscopy based on a second-order optical process, it is uniquely sensitive to detecting a molecule's vibrational fingerprints specifically at interfaces. In this microscope, a structured illumination beam generated by a spatial light modulator is used to irradiate the sample with a series of sparsifying pseudorandom patterns. The spectra associated with each pattern are then input into a reconstruction algorithm to compressively recover the full hyperspectral image cube...
May 19, 2016: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/27098655/patterning-liquids-on-inkjet-imprinted-surfaces-with-highly-adhesive-superhydrophobicity
#19
Bin Bao, Jiazhen Sun, Meng Gao, Xingye Zhang, Lei Jiang, Yanlin Song
The rapidly increasing research interest in microfluidics, microreactors and solution-processable fabrication technologies requires the development of patterning techniques to obtain large-scale functional liquid arrays. To achieve this objective, photolithography, microcontact printing and mask-based UV irradiation have been utilized to physically or chemically pattern surfaces into templates where ordered arrays of liquid materials are constructed. However, these methods require elaborately fabricated templates or expensive vacuum-deposited masks that restrict their practical applications...
May 5, 2016: Nanoscale
https://www.readbyqxmd.com/read/27035488/synaptic-compartmentalization-by-micropatterned-masking-of-a-surface-adhesive-cue-in-cultured-neurons
#20
Jae Ryun Ryu, Min Jee Jang, Youhwa Jo, Sunghoon Joo, Do Hoon Lee, Byung Yang Lee, Yoonkey Nam, Woong Sun
Functions of neuronal circuit are fundamentally modulated by its quality and quantity of connections. Assessment of synapse, the basic unit for a neuronal connection, is labor-intensive and time-consuming in conventional culture systems, due to the small size and the spatially random distribution. In the present study, we propose a novel 'synapse compartmentalization' culture system, in which synapses are concentrated at controlled locations. We fabricated a negative dot array pattern by coating the entire surface with poly-l-lysine (PLL) and subsequent microcontact printing of 1) substrates which mask positive charge of PLL (Fc, BSA and laminin), or 2) a chemorepulsive protein (Semaphorin 3F-Fc)...
June 2016: Biomaterials
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