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microcontact printing

Meiyu Gai, Johannes Frueh, Valeriya L Kudryavtseva, Rui Mao, Maxim V Kiryukhin, Gleb B Sukhorukov
Polyelectrolyte complexes (PEC) are formed by mixing the solutions of oppositely charged polyelectrolytes, which were hitherto deemed "impossible" to process, since they are infusible and brittle when dry. Here, we describe the process of fabricating free-standing micro-patterned PEC films containing array of hollow or filled microchambers by one-step casting with small applied pressure and a PDMS mould. These structures are compared with polyelectrolyte multilayers (PEM) thin films having array of hollow microchambers produced from a layer-by-layer self-assembly of the same polyelectrolytes on the same PDMS moulds...
November 10, 2016: Scientific Reports
Moritz Buhl, Matthias Tesch, Sebastian Lamping, Johanna Moratz, Armido Studer, Bart Jan Ravoo
This paper reports microcontact printing (μCP) to immobilize an alkoxyamine initiator (regulator) on glass and silicon substrates and subsequent surface-initiated alternating nitroxide-mediated copolymerization (siNMP) of hexafluoroisopropyl acrylate (HFIPA) and 7-octenylvinyl ether (OVE). The resulting patterned polymer brushes are analyzed by using atomic force microscopy (AFM). In addition, site-specific post-functionalization of the alternating polymer brushes by applying two orthogonal surface reactions is achieved with thiols and amines through μCP...
October 31, 2016: Chemistry: a European Journal
Richard Gondosiswanto, Christian A Gunawan, David Brynn Hibbert, Jason Brian Harper, Chuan Zhao
Solvent volatility represents one of the major hurdles to the reliability and reproducibility of the lab-on-a-chip systems for large scale applications. Here we demonstrate a strategy of combining non-volatile and functionalized ionic liquids with microcontact printing for fabrication of "wall-less" microreactors with high reproducibility and high throughput. A range of thiol-functionalized ionic liquids have been synthesized and used as inks for microcontact printing of ionic liquid microdroplets arrays onto gold chips...
October 26, 2016: ACS Applied Materials & Interfaces
Yujie Zhu, Jose M Moran-Mirabal
Supported lipid bilayers (SLBs) provide an excellent model system for studying structural and functional characteristics of biomembranes. Patterning model membranes on solid supports has elicited much interest because lipid bilayer arrays at cellular or sub-cellular scales provide attractive platforms for reconstituting tissue-like conditions for cell culture, and for creating simplified physiological environments to study biological processes. Phase-segregated SLB patterns can be especially useful for such studies, as the selective functionalization of the lipid phases with different lipids, receptors or proteins can be achieved to mimic the key features of plasma membrane...
October 4, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
Julie Fredonnet, Julie Foncy, Jean-Christophe Cau, Childérick Séverac, Jean Marie François, Emmanuelle Trévisiol
Microarrays are established research tools for genotyping, expression profiling, or molecular diagnostics in which DNA molecules are precisely addressed to the surface of a solid support. This study assesses the fabrication of low-density oligonucleotide arrays using an automated microcontact printing device, the InnoStamp 40(®). This automate allows a multiplexed deposition of oligoprobes on a functionalized surface by the use of a MacroStamp(TM) bearing 64 individual pillars each mounted with 50 circular micropatterns (spots) of 160 µm diameter at 320 µm pitch...
2016: Microarrays
Rituraj Dubey, Ravi Bhushan
No abstract text is available yet for this article.
October 2016: Bioanalysis
Lena F Burbulla, Kristin G Beaumont, Milan Mrksich, Dimitri Krainc
Dimitri Krainc, Milan Mrksich, and co-workers demonstrate the utility of microcontact printing technology for culturing of human neurons in defined patterns over extended periods of time on page 1894. This approach facilitates studies of neuronal development, cellular trafficking, and related mechanisms that require assessment of individual neurons and neuronal networks.
August 2016: Advanced Healthcare Materials
Jonas Albers, Andreas Offenhäusser
The central nervous system consists of an unfathomable number of functional networks enabling highly sophisticated information processing. Guided neuronal growth with a well-defined connectivity and accompanying polarity is essential for the formation of these networks. To investigate how two-dimensional protein patterns influence neuronal outgrowth with respect to connectivity and functional polarity between adjacent populations of neurons, a microstructured model system was established. Exclusive cell growth on patterned substrates was achieved by transferring a mixture of poly-l-lysine and laminin to a cell-repellent glass surface by microcontact printing...
2016: Frontiers in Bioengineering and Biotechnology
Caterina Credi, Carmela De Marco, Elena Molena, Mateu Pla Roca, Josep Samitier Martí, Joana Marques, Xavier Fernàndez-Busquets, Marinella Levi, Stefano Turri
A simple method for constructing versatile ordered biotin/avidin arrays on UV-curable perfluoropolyethers (PFPEs) is presented. The goal is the realization of a versatile platform where any biotinylated biological ligands can be further linked to the underlying biotin/avidin array. To this end, microcontact arrayer and microcontact printing technologies were developed for photobiotin direct printing on PFPEs. As attested by fluorescence images, we demonstrate that this photoactive form of biotin is capable of grafting onto PFPEs surfaces during irradiation...
October 1, 2016: Colloids and Surfaces. B, Biointerfaces
Archana Bettadapur, Gio C Suh, Nicholas A Geisse, Evelyn R Wang, Clara Hua, Holly A Huber, Alyssa A Viscio, Joon Young Kim, Julie B Strickland, Megan L McCain
In vitro models of skeletal muscle are critically needed to elucidate disease mechanisms, identify therapeutic targets, and test drugs pre-clinically. However, culturing skeletal muscle has been challenging due to myotube delamination from synthetic culture substrates approximately one week after initiating differentiation from myoblasts. In this study, we successfully maintained aligned skeletal myotubes differentiated from C2C12 mouse skeletal myoblasts for three weeks by utilizing micromolded (μmolded) gelatin hydrogels as culture substrates, which we thoroughly characterized using atomic force microscopy (AFM)...
2016: Scientific Reports
Brandon L Coyle, François Baneyx
Fusion of disulfide-constrained or linear versions of the Car9 dodecapeptide to model fluorescent proteins support their on-contact and oriented immobilization onto unmodified glass. Bound proteins can be released and the surface regenerated by incubation with l-lysine. This noncovalent chemistry enables rapid and reversibe microcontact printing of tagged proteins and speeds up the production of bicontinuous protein patterns.
May 19, 2016: Chemical Communications: Chem Comm
Lisa Marie S Ramirez, Muhan He, Shay Mailloux, Justin George, Jun Wang
Encoded microparticles are high demand in multiplexed assays and labeling. However, the current methods for the synthesis and coding of microparticles either lack robustness and reliability, or possess limited coding capacity. Here, a massive coding of dissociated elements (MiCODE) technology based on innovation of a chemically reactive off-stoichimetry thiol-allyl photocurable polymer and standard lithography to produce a large number of quick response (QR) code microparticles is introduced. The coding process is performed by photobleaching the QR code patterns on microparticles when fluorophores are incorporated into the prepolymer formulation...
June 2016: Small
Bingzhe Xu, Minshen Zhu, Wencong Zhang, Xu Zhen, Zengxia Pei, Qi Xue, Chunyi Zhi, Peng Shi
A field-effect transistor (FET) based on ultrathin Ti3 C2 -MXene micropatterns is developed by C. Zhi, P. Shi, and co-workers, as described on page 3333. The FET can be utilized for label-free probing of small molecules in typical biological environments, e.g., for fast detection of action potentials in primary neurons. This device is produced with a microcontact printing technique, harnessing the unique advantages for easy fabrication.
May 2016: Advanced Materials
Desheng Zheng, Liyang Lu, Yun Li, Kevin F Kelly, Steven Baldelli
A broad-band sum frequency generation microscope has been developed for the study of molecular monolayers on surfaces. Because sum frequency generation is a vibrational spectroscopy based on a second-order optical process, it is uniquely sensitive to detecting a molecule's vibrational fingerprints specifically at interfaces. In this microscope, a structured illumination beam generated by a spatial light modulator is used to irradiate the sample with a series of sparsifying pseudorandom patterns. The spectra associated with each pattern are then input into a reconstruction algorithm to compressively recover the full hyperspectral image cube...
May 19, 2016: Journal of Physical Chemistry Letters
Bin Bao, Jiazhen Sun, Meng Gao, Xingye Zhang, Lei Jiang, Yanlin Song
The rapidly increasing research interest in microfluidics, microreactors and solution-processable fabrication technologies requires the development of patterning techniques to obtain large-scale functional liquid arrays. To achieve this objective, photolithography, microcontact printing and mask-based UV irradiation have been utilized to physically or chemically pattern surfaces into templates where ordered arrays of liquid materials are constructed. However, these methods require elaborately fabricated templates or expensive vacuum-deposited masks that restrict their practical applications...
May 5, 2016: Nanoscale
Jae Ryun Ryu, Min Jee Jang, Youhwa Jo, Sunghoon Joo, Do Hoon Lee, Byung Yang Lee, Yoonkey Nam, Woong Sun
Functions of neuronal circuit are fundamentally modulated by its quality and quantity of connections. Assessment of synapse, the basic unit for a neuronal connection, is labor-intensive and time-consuming in conventional culture systems, due to the small size and the spatially random distribution. In the present study, we propose a novel 'synapse compartmentalization' culture system, in which synapses are concentrated at controlled locations. We fabricated a negative dot array pattern by coating the entire surface with poly-l-lysine (PLL) and subsequent microcontact printing of 1) substrates which mask positive charge of PLL (Fc, BSA and laminin), or 2) a chemorepulsive protein (Semaphorin 3F-Fc)...
June 2016: Biomaterials
Rossella Castagna, Alessandro Bertucci, Eko Adi Prasetyanto, Marco Monticelli, Dario Valter Conca, Matteo Massetti, Parikshit Pratim Sharma, Francesco Damin, Marcella Chiari, Luisa De Cola, Riccardo Bertacco
High-performing hybridization platforms fabricated by reactive microcontact printing of DNA probes are presented. Multishaped PDMS molds are used to covalently bind oligonucleotides over a functional copolymer (DMA-NAS-MAPS) surface. Printed structures with minimum width of about 1.5 μm, spaced by 10 μm, are demonstrated, with edge corrugation lower than 300 nm. The quantification of the immobilized surface probes via fluorescence imaging gives a remarkable concentration of 3.3 × 10(3) oligonucleotides/μm(2), almost totally active when used as probes in DNA-DNA hybridization assays...
April 5, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
Maria Girrbach, Ina Rink, Tatjana Ladnorg, Carlos Azucena, Stefan Heißler, Tamás Haraszti, Ute Schepers, Katja Schmitz
The attachment of neutrophils to the endothelial surface and their migration towards the site of inflammation following chemokine gradients play an essential role in the innate immune response. Chemokines adhere to glycosaminoglycans on the endothelial surface to be detected by leukocytes and trigger their movement along surface- bound gradients in a process called haptotaxis. In assays to systematically study the response of leukocytes to surface-bound compounds both the spatial arrangement of the compound as well as the mode of immobilization need to be controlled...
June 1, 2016: Colloids and Surfaces. B, Biointerfaces
Shihao Liu, Wenbo Liu, Wenyu Ji, Jing Yu, Wei Zhang, Letian Zhang, Wenfa Xie
Recent breakthroughs in quantum dot light-emitting devices (QD-LEDs) show their promise in the development of next-generation displays. However, the QD-LED with conventional ITO-based bottom emission structure is difficult to realize the high aperture ratio, electricfield-independent emission and flexible full-color displays. Hence, we demonstrate top-emitting QD-LEDs with dry microcontact printing quantum dot films. The top-emitting structure is proved to be able to accelerate the excitons radiative transition rate, then contributing to stable electroluminescent efficiency with a very low roll-off, and preventing spectra from shifting and broadening with the electric field increases...
2016: Scientific Reports
Bingzhe Xu, Minshen Zhu, Wencong Zhang, Xu Zhen, Zengxia Pei, Qi Xue, Chunyi Zhi, Peng Shi
A field-effect transistor (FET) based on ultrathin Ti3 C2 -MXene micropatterns is developed and utilized as a highly sensitive biosensor. The device is produced with the microcontact printing technique, making use of its unique advantages for easy fabrication. Using the MXene-FET device, label-free probing of small molecules in typical biological environments and fast detection of action potentials in primary neurons is demonstrated.
May 2016: Advanced Materials
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