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Keping Chen

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https://www.readbyqxmd.com/read/27701142/caspase-1-from-the-silkworm-bombyx-mori-is-involved-in-bombyx-mori-nucleopolyhedrovirus-infection
#1
Qiang Wang, Xiaoli Ju, Liang Chen, Keping Chen
Caspase-1 is one of the effector caspases in mammals that plays a central role in apoptosis. However, the lepidopteran caspase-1, especially the Bombyx mori caspase-1 (Bm-caspase-1), has not been investigated in detail. In this study, Bm-caspase-1 was identified from an expressed sequence tag database in B. mori by BLAST search. The open reading frame of Bm-caspase-1 contained 879 nucleotides and encoded 293 amino acids with a predicted molecular mass of 33 kDa. Bm-caspase-1 contained two consensus amino acid motifs of caspase cleavage sites, DEGDA and TETDG...
October 4, 2016: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/27692381/protein-determination-using-graphene-oxide-aptamer-modified-gold-nanoparticles-in-combination-with-tween-80
#2
Li Gao, Qin Li, Raoqi Li, Zebin Deng, Brendan Brady, Ni Xia, Guimin Chen, Yang Zhou, Hengchuan Xia, Keping Chen, Haixia Shi
Recently, graphene oxide (GO) has shown superiority for disease detection arising from its unique physical and chemical properties. However, proteins adsorbed on the surface of GO prevent sensitivity improvement in fluorescence-based detection methods. In this paper, a label-free method based on aptamer modified gold nanoparticles (GNPs) combined with Tween 80 was shown to solve this problem using the detection of thrombin as an example. An aptamer was designed and bound to thrombin by changing its conformation...
October 19, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27610940/high-incidence-of-ventricular-arrhythmias-in-patients-with-left-ventricular-enlargement-and-moderate-left-ventricular-dysfunction
#3
Shuang Zhao, Keping Chen, Yangang Su, Wei Hua, Jiefu Yang, Silin Chen, Zhaoguang Liang, Wei Xu, Shu Zhang
BACKGROUND: Left ventricular ejection fraction (LVEF) ≤35% is the cutoff value used to identify patients at high risk of sudden cardiac death. However, whether patients who have moderate left ventricular dysfunction and left ventricular enlargement (LVE) have a high risk of ventricular arrhythmias (VAs) and cardiac death is unclear. HYPOTHESIS: Patients with moderate left ventricular dysfunction and LVE may have a high risk of VAs. METHODS: This study retrospectively analyzed the data of 853 patients who have an implantable cardioverter-defibrillator/cardiac resynchronization therapy cardioverter-defibrillator equipped with home monitoring...
September 9, 2016: Clinical Cardiology
https://www.readbyqxmd.com/read/27513293/microrna-let-7b-inhibits-keratinocyte-migration-in-cutaneous-wound-healing-by-targeting-igf2bp2
#4
Yan Wu, Julia Li Zhong, Ning Hou, Yaolan Sun, Benting Ma, Muhammad Farrukh Nisar, Yan Teng, Zhaoli Tan, Keping Chen, Youliang Wang, Xiao Yang
Wound healing is a complex process involves proliferation and migration of keratinocyte for closure of epidermal injuries. A member of microRNA family let-7b, have been expressed in mammalian skin but its exact role in keratinocyte migration is still not in knowledge. Here we showed that let-7b regulates keratinocyte migration by targeting the insulin-like growth factor IGF2BP2. Overexpression of let-7b led to reduced HaCaT cell migration while knockdown of let-7b resulted in enhanced migration. Furthermore, let-7b was decreased during wound healing in wild type mice, which led us to construct the transgenic mice with overexpression of let-7b in skin...
August 11, 2016: Experimental Dermatology
https://www.readbyqxmd.com/read/27501329/nuclear-gsk3%C3%AE-promotes-tumorigenesis-by-phosphorylating-kdm1a-and-inducing-its-deubiquitylation-by-usp22
#5
Aidong Zhou, Kangyu Lin, Sicong Zhang, Yaohui Chen, Nu Zhang, Jianfei Xue, Zhongyong Wang, Kenneth D Aldape, Keping Xie, James R Woodgett, Suyun Huang
Emerging evidence has shown that GSK3β plays oncogenic roles in multiple tumour types; however, the underlying mechanisms remain largely unknown. Here, we show that nuclear GSK3β is responsible for the accumulation of the histone demethylase KDM1A and critically regulates histone H3K4 methylation during tumorigenesis. GSK3β phosphorylates KDM1A Ser683 upon priming phosphorylation of KDM1A Ser687 by CK1α. Phosphorylation of KDM1A induces its binding with and deubiquitylation by USP22, leading to KDM1A stabilization...
September 2016: Nature Cell Biology
https://www.readbyqxmd.com/read/27245166/mir-130a-regulates-neurite-outgrowth-and-dendritic-spine-density-by-targeting-mecp2
#6
Yunjia Zhang, Mengmeng Chen, Zilong Qiu, Keping Hu, Warren McGee, Xiaoping Chen, Jianghong Liu, Li Zhu, Jane Y Wu
MicroRNAs (miRNAs) are critical for both development and function of the central nervous system. Significant evidence suggests that abnormal expression of miRNAs is associated with neurodevelopmental disorders. MeCP2 protein is an epigenetic regulator repressing or activating gene transcription by binding to methylated DNA. Both loss-of-function and gain-of-function mutations in the MECP2 gene lead to neurodevelopmental disorders such as Rett syndrome, autism and MECP2 duplication syndrome. In this study, we demonstrate that miR-130a inhibits neurite outgrowth and reduces dendritic spine density as well as dendritic complexity...
July 2016: Protein & Cell
https://www.readbyqxmd.com/read/27093342/heparin-binding-epidermal-growth-factor-like-growth-factor-as-a-potent-target-for-breast-cancer-therapy
#7
Caixia Lian, Lingling Ruan, Dongsheng Shang, Yanfang Wu, Yongjin Lu, Peng Lü, Yuhua Yang, Yajun Wei, Xiaojing Dong, Dewan Ren, Keping Chen, Hanqing Liu, Zhigang Tu
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) belongs to the EGF family and exhibits its activity after binding to its receptors in autocrine, paracrine, and juxtacrine interactions. HB-EGF plays important roles in several biological and pathological processes, such as wound healing, blastocyst implantation, atherosclerosis, and heart development. Clinical studies have shown that HB-EGF is closely correlated with tumorigenesis, metastasis, and drug resistance in breast cancer. Specifically, targeted inhibition of HB-EGF improves the therapeutic efficacy and suppresses the tumor progression...
April 2016: Cancer Biotherapy & Radiopharmaceuticals
https://www.readbyqxmd.com/read/26979609/suppression-of-bm-caspase-1-expression-in-bmn-cells-enhances-recombinant-protein-production-in-a-baculovirus-expression-vector-system
#8
Qiang Wang, Yang Zhou, Keping Chen, Xiaoli Ju
Baculovirus expression vector systems (BEVSs) have been widely used for recombinant protein production. Many studies have tried to improve the recombinant protein production by either modification of BEVSs vectors or by cell line selection. In this study, using a modified shRNA vector, we established a stable Bombyx mori cell line that significantly inhibits expression of caspase-1 after selection. We further compared cell proliferation and viability between caspase-1-suppressed cells and control cells, and found that there is no significant difference in these stable cell lines...
May 2016: Molecular Biotechnology
https://www.readbyqxmd.com/read/26883101/synergic-interaction-between-amyloid-precursor-protein-and-neural-cell-adhesion-molecule-promotes-neurite-outgrowth
#9
Keping Chen, Huixia Lu, Tianli Gao, Xiulei Xue, Chunling Wang, Fengqin Miao
Alzheimer's disease (AD) is one of the most common neurodegenerative diseases worldwide. The main features of AD are the pathological changes of density and distribution of intracellular neurofibrillary tangles (NFT) and extracellular amyloid plaques. The processing of amyloid beta precursor protein (APP) to β-amyloid peptide (Aβ) is one of the critical events in the pathogenesis of AD. In this study, we evaluated the role of the interaction of neural cell adhesion molecule (NCAM) and APP in neurite outgrowth using two different experimental systems: PC12E2 cells and hippocampal neurons that were isolated from wild type, APP knock-in and APP knock-out mice...
March 22, 2016: Oncotarget
https://www.readbyqxmd.com/read/26842120/the-role-of-variability-in-night-time-mean-heart-rate-on-the-prediction-of-ventricular-arrhythmias-and-all-cause-mortality-in-implantable-cardioverter-defibrillator-patients
#10
Shuang Zhao, Keping Chen, Yangang Su, Wei Hua, Jielin Pu, Hui Li, Yan Dai, Min Tang, Xiaohan Fan, Yunzi Zhao, Xiaolin Zheng, Chi Cai, Zeyi Li, Shu Zhang
AIMS: This study was to use implantable cardioverter defibrillator (ICD) home monitoring (HM) feature to evaluate the role of mean night-time heart rate (MNHR) in the occurrence of ventricular arrhythmias (VAs) and mortality. METHODS AND RESULTS: This study retrospectively analysed clinical and ICD device data in 318 ICD patients. Data of the first 30-day MNHR (recorded 02:00-06:00 am) by HM were collected. The average and standard deviation of 30-day MNHR (AVHR and SDHR, respectively) were then determined in each patient...
October 2015: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/26830889/multiple-leadless-pacemakers-implanted-in-the-right-ventricle-of-swine
#11
Keping Chen, Xiaolin Zheng, Yan Dai, Hao Wang, Yue Tang, Tingyu Lan, Jinping Zhang, Yi Tian, Baojie Zhang, Xiaohong Zhou, Matthew Bonner, Shu Zhang
AIMS: The miniaturized leadless pacemaker has recently emerged as a bradycardia therapy in humans, and many patients may need at least two pacemakers in their lifetime. Thus, the present study assessed the effect of two leadless pacemakers in the right ventricle (RV) on cardiac function in a swine model. METHODS AND RESULTS: Fourteen mini pigs were chronically studied. Each animal received two sequential leadless pacemakers in the RV with 1 month between two separate implantations...
January 31, 2016: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/26698122/differential-expression-of-hepatic-genes-of-the-greater-horseshoe-bat-rhinolophus-ferrumequinum-between-the-summer-active-and-winter-torpid-states
#12
COMPARATIVE STUDY
Yanhong Xiao, Yonghua Wu, Keping Sun, Hui Wang, Bing Zhang, Shuhui Song, Zhenglin Du, Tinglei Jiang, Limin Shi, Lei Wang, Aiqing Lin, Xinke Yue, Chenji Li, Tingting Chen, Jiang Feng
Hibernation is one type of torpor, a hypometabolic state in heterothermic mammals, which can be used as an energy-conservation strategy in response to harsh environments, e.g. limited food resource. The liver, in particular, plays a crucial role in adaptive metabolic adjustment during hibernation. Studies on ground squirrels and bears reveal that many genes involved in metabolism are differentially expressed during hibernation. Especially, the genes involved in carbohydrate catabolism are down-regulated during hibernation, while genes responsible for lipid β-oxidation are up-regulated...
2015: PloS One
https://www.readbyqxmd.com/read/26686834/regulation-of-bmbdv-ns1-by-phosphorylation-impact-of-mutagenesis-at-consensus-phosphorylation-sites-on-atpase-activity-and-cytopathic-effects
#13
Guohui Li, Mangmang Li, Wu Xu, Qian Zhou, Zhaoyang Hu, Qi Tang, Keping Chen, Qin Yao
Bombyx mori bidensovirus (BmBDV) is a single-stranded DNA virus belonging to the Bidensovirus genus, Bidnaviridae family. Previous studies showed that parvovirus nonstructural protein 1 (NS1) contains endonuclease, helicase, and ATPase activities and that these activities are regulated by serine/threonine phosphorylation. We have reported that residue Thr-184 site of BmBDV NS1 is phosphorylated, and that residues of Thr-181 and Thr-191 are potentially phosphorylated. However, whether phosphorylation affects BmBDV NS1 activities remains unclear...
January 2016: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/26594239/lignin-degradation-in-corn-stalk-by-combined-method-of-h2o2-hydrolysis-and-aspergillus-oryzae-cgmcc5992-liquid-state-fermentation
#14
Zhicai Zhang, Lili Xia, Feng Wang, Peng Lv, Maxiaoqi Zhu, Jinhua Li, Keping Chen
BACKGROUND: Lignin peroxidase (LiP) is the primary enzyme responsible for lignin degradation. In our previous work, in order to shorten the pretreatment time and increase the lignin degradation, we have pretreated the corn stalk (CS) using a combination of Aspergillus oryzae CGMCC 5992 solid-state fermentation and H2O2 treatment. RESULTS: In the present study, one-factor-at-a-time design and response surface design were applied to optimize the nutritional constituents for LiP production in liquid-state fermentation by A...
2015: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/26505809/an-investigation-and-evaluation-on-species-and-characteristics-of-pathogenic-microorganisms-in-chinese-local-hospital-settings
#15
Liang Chen, Xiaoli Lü, Weiping Cao, Chunxia Zhang, Rongfa Xu, Xu Meng, Keping Chen
There are currently great concerns about the level of bacterial contamination in hospitals, as well as resistance to antimicrobial agents. The species and characteristics of microbes in Chinese hospitals are closely related to healthcare safety and the prevention and control of infections. However, data on the exposure of patients to microbes in Chinese hospitals are limited. The present study investigated the genera of microorganisms in Chinese hospitals. We evaluated their characteristics, such as antibiotic susceptibility, tolerance to disinfectants, and toxicity, using silkworms (Bombyx mori) as an animal model...
December 2015: Microbial Pathogenesis
https://www.readbyqxmd.com/read/26474303/molecular-and-physiological-characterization-of-two-novel-multirepeat-%C3%AE-thymosins-from-silkworm-bombyx-mori
#16
Shangshang Ma, Zhiqiong Kang, Peng Lü, Yanhua Yang, Qin Yao, Hengchuan Xia, Keping Chen
β-thymosin plays important roles in the development of the lymphatic system and the central nervous system in vertebrates. However, its role and function in invertebrates remain much less explored. Here, we firstly isolated a gene encoding β-thymosin in silkworm (Bombyx mori L.). Interestingly, this gene encodes two polypeptides, named as BmTHY1 and BmTHY2, via two different modes of RNA splicing. The recombinant proteins fused with an N-term GST tag were over-expressed in Escherichia coli (E. coli) and further purified to near homogenity to prepare mouse antibodies...
2015: PloS One
https://www.readbyqxmd.com/read/26456472/closely-related-taxa-influence-woody-species-discrimination-via-dna-barcoding-evidence-from-global-forest-dynamics-plots
#17
Nancai Pei, David L Erickson, Bufeng Chen, Xuejun Ge, Xiangcheng Mi, Nathan G Swenson, Jin-Long Zhang, Frank A Jones, Chun-Lin Huang, Wanhui Ye, Zhanqing Hao, Chang-Fu Hsieh, Shawn Lum, Norman A Bourg, John D Parker, Jess K Zimmerman, William J McShea, Ida C Lopez, I-Fang Sun, Stuart J Davies, Keping Ma, W John Kress
To determine how well DNA barcodes from the chloroplast region perform in forest dynamics plots (FDPs) from global CTFS-ForestGEO network, we analyzed DNA barcoding sequences of 1277 plant species from a wide phylogenetic range (3 FDPs in tropics, 5 in subtropics and 5 in temperate zone) and compared the rates of species discrimination (RSD). We quantified RSD by two DNA barcode combinations (rbcL + matK and rbcL + matK + trnH-psbA) using a monophyly-based method (GARLI). We defined two indexes of closely-related taxa (Gm/Gt and S/G ratios) and correlated these ratios with RSD...
2015: Scientific Reports
https://www.readbyqxmd.com/read/26411436/prediction-of-ventricular-arrhythmia-events-in-ischemic-heart-disease-patients-with-implantable-cardioverter-defibrillators
#18
Tianjie Feng, Shu Zhang, Keping Chen, Wei Hua, Xiaoqing Ren
The aim of the study was to exam the prediction of ventricular arrhythmia events in ischemic heart disease patients with implantable cardioverter-defibrillators (ICD). A total of 123 consecutive patients confirmed ischemia heart disease with ICD were examined. After device implantation, the occurrence of appropriate ICD therapy was noted. Patients were divided into two groups according to the ventricular arrhythmia occurrence. Patients with ventricular arrhythmia occurrence had a significantly great incidence of atrial fibrillation history compare to the no-ventricular arrhythmia occurrence group (8 vs...
October 2015: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/26318029/expression-and-display-of-a-novel-thermostable-esterase-from-clostridium-thermocellum-on-the-surface-of-bacillus-subtilis-using-the-cotb-anchor-protein
#19
Huayou Chen, Tianxi Zhang, Jinru Jia, Ake Vastermark, Rui Tian, Zhong Ni, Zhi Chen, Keping Chen, Shengli Yang
Esterases expressed in microbial hosts are commercially valuable, but their applications are limited due to high costs of production and harsh industrial processes involved. In this study, the esterase-DSM (from Clostridium thermocellum) was expressed and successfully displayed on the spore surface, and the spore-associated esterase was confirmed by western blot analysis and activity measurements. The optimal temperature and pH of spore surface-displayed DSM was 60 and 8.5 °C, respectively. It also demonstrates a broad temperature and pH optimum in the range of 50-70, 7-9...
November 2015: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/26091630/necroptotic-cells-release-find-me-signal-and-are-engulfed-without-proinflammatory-cytokine-production
#20
Qiang Wang, Xiaoli Ju, Yang Zhou, Keping Chen
Necroptosis is a form of caspase-independent programmed cell death which is mediated by the RIP1-RIP3 complex. Although phagocytosis of apoptotic cells has been extensively investigated, how necroptotic cells are engulfed has remained elusive. Here, we investigated how necroptotic cells attracted and were engulfed by macrophages. We found that necroptotic cells induced the migration of THP-1 cells in a transwell migration assay. Further analysis showed that ATP released from necroptotic cells acted as a find-me signal that induced the migration of THP-1 cells...
November 2015: In Vitro Cellular & Developmental Biology. Animal
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