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https://www.readbyqxmd.com/read/28711247/ratio-of-dietary-rumen-degradable-protein-to-rumen-undegradable-protein-affects-nitrogen-partitioning-but-does-not-affect-the-bovine-milk-proteome-produced-by-mid-lactation-holstein-dairy-cows
#1
R Tacoma, J Fields, D B Ebenstein, Y-W Lam, S L Greenwood
Little is known about the bovine milk proteome or whether it can be affected by diet. The objective of this study was to determine if the dietary rumen degradable protein (RDP):rumen undegradable protein (RUP) ratio could alter the bovine milk proteome. Six Holstein cows (parity: 2.5 ± 0.8) in mid lactation were blocked by days in milk (80 ± 43 d in milk) and milk yield (57.5 ± 6.0 kg) and randomly assigned to treatment groups. The experiment was conducted as a double-crossover design consisting of three 21-d periods...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28710878/6-mercaptopurine-modifies-cerebrospinal-fluid-t-cell-abnormalities-in-pediatric-opsoclonus-myoclonus-as-steroid-sparer
#2
Michael R Pranzatelli, Elizabeth D Tate, Tyler J Allison
The purpose was to evaluate the capacity of 6-mercaptopurine (6-MP), a known immunosuppressant, to normalize cerebrospinal fluid (CSF) lymphocyte frequencies in opsoclonus-myoclonus syndrome (OMS) and function as a steroid sparer. CSF and blood lymphocytes were immunophenotyped in 11 children with OMS (without CSF B cell expansion), using a comprehensive panel of cell surface adhesion, activation, and maturation markers by flow cytometry, and referenced to 18 pediatric controls. Drug metabolites, lymphocyte counts, and liver function tests were used clinically to monitoring therapeutic range and toxicity...
July 15, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28705994/wearable-brain-imaging-with-multi-modal-physiological-recording
#3
Gary E Strangman, Vladimir Ivkovic, Quan Zhang
The brain is a central component of cognitive and physical human performance. Measures including functional brain activation, cerebral perfusion, cerebral oxygenation, evoked electrical responses, and resting hemodynamic and electrical activity are all related to, or can predict health status or performance decrements. However, measuring brain physiology typically requires large, stationary machines that are not suitable for mobile or self-monitoring. Moreover, when individuals are ambulatory, systemic physiological fluctuations-e...
July 13, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28702019/functional-resilience-and-response-to-a-dietary-additive-kefir-in-models-of-foregut-and-hindgut-microbial-fermentation-in-vitro
#4
Gabriel de la Fuente, Eleanor Jones, Shann Jones, Charles J Newbold
Stability in gut ecosystems is an important area of study that impacts on the use of additives and is related with several pathologies. Kefir is a fermented milk drink made with a consortium of yeast and bacteria as a fermentation starter, of which the use as additive in companion and livestock animals has increased in the last few years. To investigate the effect of kefir milk on foregut and hindgut digestive systems, an in vitro approach was followed. Either rumen fluid or horse fecal contents were used as a microbial inoculate and the inclusion of kefir (fresh, autoclaved, or pasteurized) was tested...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28692957/passive-wearable-skin-patch-sensor-measures-limb-hemodynamics-based-on-electromagnetic-resonance
#5
Kim Cluff, Ryan A Becker, Balakumar Jayakumar, Kiyun Han, Ernie Condon, Kenneth Dudley, George Szatkowski, Iraklis I Pipinos, Ryan Z Amick, Jeremy Patterson
OBJECTIVE: The objectives of this study were to design and develop an open circuit electromagnetic resonant skin patch sensor, characterize the fluid volume and resonant frequency relationship and investigate the sensor's ability to measure limb hemodynamics and pulse volume waveform features. METHODS: The skin patch was designed from an open circuit electromagnetic resonant sensor comprised of a single baseline trace of copper configured into a square planar spiral which had a self-resonating response when excited by an external radio frequency (RF) sweep...
July 4, 2017: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28686319/magnetic-resonance-thermometry-of-flowing-blood
#6
Chinthaka C Heyn, Jonathan Bishop, Kyle Duffin, Wayne Lee, Jun Dazai, Shoshana Spring, Brian J Nieman, John G Sled
Blood temperature is a key determinant of tissue temperature and can be altered under normal physiological states, such as exercise, in diseases such as stroke or iatrogenically in therapies which modulate tissue temperature, such as therapeutic hypothermia. Currently available methods for the measurement of arterial and venous temperatures are invasive and, for small animal models, are impractical. Here, we present a methodology for the measurement of intravascular and tissue temperature by magnetic resonance imaging (MRI) using the lanthanide agent TmDOTMA(-) (DOTMA, tetramethyl-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid; Tm, thulium)...
July 7, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28682837/closed-loop-and-decision-assist-guided-fluid-therapy-of-human-hemorrhage
#7
Gabriel Hundeshagen, George C Kramer, Nicole Ribeiro Marques, Michael G Salter, Aristides K Koutrouvelis, Husong Li, Daneshvari R Solanki, Alexander Indrikovs, Roger Seeton, Sheryl N Henkel, Michael P Kinsky
OBJECTIVES: We sought to evaluate the efficacy, efficiency, and physiologic consequences of automated, endpoint-directed resuscitation systems and compare them to formula-based bolus resuscitation. DESIGN: Experimental human hemorrhage and resuscitation. SETTING: Clinical Research Laboratory. SUBJECTS: Healthy volunteers. INTERVENTIONS: Subjects (n = 7) were subjected to hemorrhage and underwent a randomized fluid resuscitation scheme on separate visits 1) formula-based bolus resuscitation; 2) semiautonomous (decision assist) fluid administration; and 3) fully autonomous (closed loop) resuscitation...
July 4, 2017: Critical Care Medicine
https://www.readbyqxmd.com/read/28680389/post-mortem-validation-of-mri-identified-veins-on-the-surface-of-the-cerebral-cortex-as-potential-landmarks-for-neurosurgery
#8
Günther Grabner, Thomas Haider, Mark Glassner, Alexander Rauscher, Hannes Traxler, Siegfried Trattnig, Simon D Robinson
Background and Objective: Image-guided neurosurgery uses information from a wide spectrum of methods to inform the neurosurgeon's judgement about which tissue to resect and which to spare. Imaging data are registered to the patient's skull so that they correspond to the intraoperative macro- and microscopic view. The correspondence between imaging and optical systems breaks down during surgery, however, as a result of cerebro-spinal fluid drain age, tissue resection, and gravity-based brain shift. In this work we investigate whether a map of surface veins, automatically segmented from MRI, could serve as additional reference system...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28676955/a-novel-approach-for-acoustic-estimation-of-neck-fluid-volume-between-men-and-women
#9
Mehrnaz Shokrollahi, Frank Rudzicz, Daniel Vena, T Douglas Bradley, Azadeh Yadollahi
Obstructive Sleep apnea can be caused by fluid shift from the legs to the neck that narrows the upper airway (UA) and contributes to changes in tracheal sound. Tracheal sound is generated from the turbulent airflow in the pharynx and respiratory airways and it has recently been used to estimate increases in neck fluid volume (NFV). However, tracheal sound is also highly variable among people, especially across the sexes. In this paper, a novel method is proposed to select tracheal sound features towards estimating NFV in men and women separately...
July 5, 2017: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/28676821/intraoperative-imaging-modalities-and-compensation-for-brain-shift-in-tumor-resection-surgery
#10
REVIEW
Siming Bayer, Andreas Maier, Martin Ostermeier, Rebecca Fahrig
Intraoperative brain shift during neurosurgical procedures is a well-known phenomenon caused by gravity, tissue manipulation, tumor size, loss of cerebrospinal fluid (CSF), and use of medication. For the use of image-guided systems, this phenomenon greatly affects the accuracy of the guidance. During the last several decades, researchers have investigated how to overcome this problem. The purpose of this paper is to present a review of publications concerning different aspects of intraoperative brain shift especially in a tumor resection surgery such as intraoperative imaging systems, quantification, measurement, modeling, and registration techniques...
2017: International Journal of Biomedical Imaging
https://www.readbyqxmd.com/read/28675062/lung-regeneration-using-amniotic-fluid-mesenchymal-stem-cells
#11
Alireza Azargoon, Babak Negahdari
Respiratory diseases, such as chronic obstructive pulmonary disease (COPD), pulmonary hypertension and lung fibrosis, are yet a major challenge in the world and they result in irreversible structural lung damage. Lung transplantation as the only therapeutic option face some major challenges like graft rejection and cancer, arising as a result of immunosuppression. A low survival rate faced by lung transplantation patients is presently limited to approximately 5 years. Lungs shortage therefore calls for a mechanism that would increase the availability of suitable organs for transplantation...
July 4, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28674514/aspects-on-the-physiological-and-biochemical-foundations-of-neurocritical-care
#12
REVIEW
Carl-Henrik Nordström, Lars-Owe Koskinen, Magnus Olivecrona
Neurocritical care (NCC) is a branch of intensive care medicine characterized by specific physiological and biochemical monitoring techniques necessary for identifying cerebral adverse events and for evaluating specific therapies. Information is primarily obtained from physiological variables related to intracranial pressure (ICP) and cerebral blood flow (CBF) and from physiological and biochemical variables related to cerebral energy metabolism. Non-surgical therapies developed for treating increased ICP are based on knowledge regarding transport of water across the intact and injured blood-brain barrier (BBB) and the regulation of CBF...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28668981/implicit-valuation-of-the-near-miss-is-dependent-on-outcome-context
#13
Parker J Banks, Matthew S Tata, Patrick J Bennett, Allison B Sekuler, Aaron J Gruber
Gambling studies have described a "near-miss effect" wherein the experience of almost winning increases gambling persistence. The near-miss has been proposed to inflate the value of preceding actions through its perceptual similarity to wins. We demonstrate here, however, that it acts as a conditioned stimulus to positively or negatively influence valuation, dependent on reward expectation and cognitive engagement. When subjects are asked to choose between two simulated slot machines, near-misses increase valuation of machines with a low payout rate, whereas they decrease valuation of high payout machines...
July 1, 2017: Journal of Gambling Studies
https://www.readbyqxmd.com/read/28666451/autologous-chondrocyte-implantation-derived-synovial-fluids-display-distinct-responder-and-non-responder-proteomic-profiles
#14
Charlotte H Hulme, Emma L Wilson, Mandy J Peffers, Sally Roberts, Deborah M Simpson, James B Richardson, Pete Gallacher, Karina T Wright
BACKGROUND: Autologous chondrocyte implantation (ACI) can be used in the treatment of focal cartilage injuries to prevent the onset of osteoarthritis (OA). However, we are yet to understand fully why some individuals do not respond well to this intervention. Identification of a reliable and accurate biomarker panel that can predict which patients are likely to respond well to ACI is needed in order to assign the patient to the most appropriate therapy. This study aimed to compare the baseline and mid-treatment proteomic profiles of synovial fluids (SFs) obtained from responders and non-responders to ACI...
June 30, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28664946/the-influence-of-polyanion-molecular-weight-on-polyelectrolyte-multilayers-at-surfaces-elasticity-and-susceptibility-to-saloplasticity-of-strongly-dissociated-synthetic-polymers-at-fluid-fluid-interfaces
#15
Ashley D Cramer, Wen-Fei Dong, Natalie L Benbow, Jessie L Webber, Marta Krasowska, David A Beattie, James K Ferri
We studied the interfacial mechanical properties of polyelectrolyte multilayer assemblies of poly(diallylamine hydrochloride) (PAH) and poly(4-styrenesulfonate)sodium salt (PSS) at the air-water interface using axisymmetric drop shape analysis (ADSA) during hydrostatic inflation as a function of aqueous salt concentration and two different polyanion molecular weights (Mw ∼ 13 and 70 kDa). Surface elastic moduli (Gs) ranged from 50 to 300 mN m(-1). Using the measured film thickness, the bulk moduli (G) ranged from 10 to 90 MPa consistent with elastomeric solids...
June 30, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28659721/mtdna-as-a-cancer-marker-a-finally-closed-chapter
#16
REVIEW
Elmar Kirches
Sequence alterations of the mitochondrial DNA (mtDNA) have been identified in many tu-mor types. Their nature is not entirely clear. Somatic mutation or shifts of heteroplasmic mtDNA vari-ants may play a role. These sequence alterations exhibit a sufficient frequency in all tumor types investi-gated thus far to justify their use as a tumor marker. This statement is supported by the high copy num-ber of mtDNA, which facilitates the detection of aberrant tumor-derived DNA in bodily fluids. This will be of special interest in tumors, which release a relatively high number of cells into bodily fluids, which are easily accessible, most strikingly in urinary bladder carcinoma...
June 2017: Current Genomics
https://www.readbyqxmd.com/read/28649624/spaceflight-induced-neuroplasticity-in-humans-as-measured-by-mri-what-do-we-know-so-far
#17
Angelique Van Ombergen, Steven Laureys, Stefan Sunaert, Elena Tomilovskaya, Paul M Parizel, Floris L Wuyts
Space travel poses an enormous challenge on the human body; microgravity, ionizing radiation, absence of circadian rhythm, confinement and isolation are just some of the features associated with it. Obviously, all of the latter can have an impact on human physiology and even induce detrimental changes. Some organ systems have been studied thoroughly under space conditions, however, not much is known on the functional and morphological effects of spaceflight on the human central nervous system. Previous studies have already shown that central nervous system changes occur during and after spaceflight in the form of neurovestibular problems, alterations in cognitive function and sensory perception, cephalic fluid shifts and psychological disturbances...
2017: NPJ Microgravity
https://www.readbyqxmd.com/read/28646608/fluid-filling-of-the-digestive-tract-for-improved-proton-resonance-frequency-shift-based-mr-thermometry-in-the-pancreas
#18
Cyril J Ferrer, Lambertus W Bartels, Marijn van Stralen, Baudouin Denis de Senneville, Chrit T W Moonen, Clemens Bos
PURPOSE: To demonstrate that fluid filling of the digestive tract improves the performance of respiratory motion-compensated proton resonance frequency shift (PRFS)-based magnetic resonance (MR) thermometry in the pancreas. MATERIALS AND METHODS: In seven volunteers (without heating), we evaluated PRFS thermometry in the pancreas with and without filling of the surrounding digestive tract. All data acquisition was performed at 1.5T, then all datasets were analyzed and compared with three different PRFS respiratory motion-compensated thermometry methods: gating, multibaseline, and referenceless...
June 24, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28638626/effects-of-liposomal-curcumin-on-five-opportunistic-bacterial-strains-found-in-the-equine-hindgut-preliminary-study
#19
S D Bland, E B Venable, J L McPherson, R L Atkinson
BACKGROUND: The horse intestinal tract is sensitive and contains a highly complex microbial population. A shift in the microbial population can lead to various issues such as inflammation and colic. The use of nutraceuticals in the equine industry is on the rise and curcumin is thought to possess antimicrobial properties that may help to minimize the proliferation of opportunistic bacteria. METHODS: Four cecally-cannulated horses were utilized to determine the optimal dose of liposomal-curcumin (LIPC) on reducing Streptococcus bovis/equinus complex (SBEC), Escherichia coli K-12, Escherichia coli general, Clostridium difficile, and Clostridium perfringens in the equine hindgut without adversely affecting cecal characteristics...
2017: Journal of Animal Science and Technology
https://www.readbyqxmd.com/read/28632265/3d-printed-conformal-microfluidics-for-isolation-and-profiling-of-biomarkers-from-whole-organs
#20
Manjot Singh, Yuxin Tong, Kelly Webster, Ellen Cesewski, Alexander P Haring, Sahil Laheri, Bill Carswell, Timothy J O'Brien, Charles H Aardema, Ryan S Senger, John L Robertson, Blake N Johnson
The ability to interface microfluidic devices with native complex biological architectures, such as whole organs, has the potential to shift the paradigm for the study and analysis of biological tissue. Here, we show 3D printing can be used to fabricate bio-inspired conformal microfluidic devices that directly interface with the surface of whole organs. Structured-light scanning techniques enabled the 3D topographical matching of microfluidic device geometry to porcine kidney anatomy. Our studies show molecular species are spontaneously transferred from the organ cortex to the conformal microfluidic device in the presence of fluid flow through the organ-conforming microchannel...
June 20, 2017: Lab on a Chip
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